MD09G1215300.v1.1

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005617
Physical Location & Seq
Reverse (-)
21260284 .. 21264623
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1215300.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGATGTTCCCTCTGCAGCACCTAATAGTGACACCCAAGGTGACCAATTTCCTTTGCTACTAGAGCAGATGGAAAGTCATAACAATTGTGTGCACGTAATTGATGTAACAAGGAATTATGATGCTTCATCAAGTACATCTAGTGATGACCAACGTCCTAGAGTGGACATGCCCCAGCATGAAGACAGACCATCAGCGAGTACACATGCCCCGACTTATCTAGGTGCATCATTTTCATCAAACAGATTAAATTCTAGGAATTCCTCATTTGTGAGGAGACGTGATGGGTATAGTCATCGCCGAAGAAGCCCTTTAAATTCAGGGTTATGGATTTCCGTTGAGCTTGTTGTCACTGTGAGTCAGATTACAGCATCTATTGTTGTTTTGTCACTTTCAACAAACGAAGATCCACAAGCCCCTTTGTTTGCATGGGTTGTGGGTTATGCATCAGGTTGTGTTGCAACACTTCCTGTTCTTTACTGGAGGTTCCGTAACCGCAACAGGGCTGCTGAGCAAGAATCCATTCAATCGCATCAAGGGTCTTCTGTAAGCAGTCCTGAACCAACGTCTTATACAGCTATTTCAATTACTCAAGCTTCAGATGAAGAGCACAATCGCACCTCCGAAACGGTTACAACTAACCCTCAAATTGGAGGACCCTTAAGTGCAAGAATTAATGGGTTAGTGGATCATTTCAAGATGGCCTTAGATTGCTTCTTTGCAGTCTGGTTTGTTGTAGGCAATGTGTGGATTTTTGGAAGTCACTCTTCTCCTTCTGATGCTCCTAAACTGTACAAGTTATGCATAGTGTTCCTTACCTTCAGCTGTATTGGATATGCAATGCCATTCATACTATGTGCAACAATTTGCTGCTGCCTACCTTGCATCATTTCTGTCCTCGGATTTCGAGAGGATCTTTCTCAGACTAGAGGAGCCACCGTGGAATCAATTAATGCTCTCCCGACGTACAAATTCAAGTTGAAGGAAAACGAGAGCAATGATGATCAGGAGGGCAACCTAGGTAGTGAATGTGGCCTCTTGGCTGCAGGGACAGAAAAGGAGCGTGAGATATCTGGAGAAGATGCGGTTTGTTGTATTTGCTTGGCAAAGTATGCAGATGACGATGAGCTACGGGAGCTACCATGTTTACATGTCTTTCACGTGGAGTGTGTTGACAAATGGTTGAAAATAAATGCATCGTGTCCCCTTTGCAAAAGTGAGGCCGGAGAGAGCAGCGGCGAGGCCAGGGACTCCAGCCAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

422

Amino Acids

46.24

Weight (kDa)

5.08

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 364 - 405 7.9e-14 Ring finger domain
zf-RING_11 PF17123 364 - 391 2.2e-08 RING-like zinc finger
zf-rbx1 PF12678 364 - 405 1.3e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 364 - 404 3.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 364 - 404 5.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 496, 1085, 1236
AclWI GGATC 3 cut(s) 401, 696, 921
AcsI RAATTY 4 cut(s) 250, 259, 316, 971
AcuI CTGAAG 2 cut(s) 582, 805
AcvI CACGTG 1 cut(s) 1162
AfaI GTAC 4 cut(s) 136, 202, 794, 968
AfiI CCNNNNNNNGG 2 cut(s) 502, 650
AflII CTTAAG 1 cut(s) 661
AflIII ACRYGT 1 cut(s) 1150
AgsI TTSAA 7 cut(s) 396, 527, 585, 697, 976, 982, 1186
AjiI CACGTC 1 cut(s) 281
AjnI CCWGG 1 cut(s) 1244
AluBI AGCT 6 cut(s) 343, 578, 596, 825, 1129, 1138
AluI AGCT 6 cut(s) 343, 578, 596, 825, 1129, 1138
Alw21I GWGCWC 2 cut(s) 96, 612
Alw26I GTCTC 1 cut(s) 271
Alw44I GTGCAC 1 cut(s) 92
AlwI GGATC 3 cut(s) 401, 696, 921
AoxI GGCC 4 cut(s) 702, 1033, 1221, 1242
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 6 cut(s) 17, 506, 870, 873, 1043, 1233
ApoI RAATTY 4 cut(s) 250, 259, 316, 971
AseI ATTAAT 2 cut(s) 675, 951
Asp700I GAANNNNTTC 1 cut(s) 522
AspA2I CCTAGG 1 cut(s) 1018
AspS9I GGNCC 1 cut(s) 656
AsuHPI GGTGA 1 cut(s) 53
AvaII GGWCC 1 cut(s) 656
AvrII CCTAGG 1 cut(s) 1018
BaeGI GKGCMC 1 cut(s) 96
BbrPI CACGTG 1 cut(s) 1162
BbsI GAAGAC 2 cut(s) 189, 534
Bbv12I GWGCWC 2 cut(s) 96, 612
BbvI GCAGC 6 cut(s) 29, 493, 857, 860, 1030, 1245
BccI CCATC 4 cut(s) 64, 199, 278, 694
BciT130I CCWGG 1 cut(s) 1246
BclI TGATCA 1 cut(s) 1003
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 7 cut(s) 62, 141, 159, 221, 255, 927, 1019
BfmI CTRYAG 2 cut(s) 15, 1044
BfrI CTTAAG 1 cut(s) 661
BisI GCNGC 7 cut(s) 18, 507, 871, 874, 1044, 1234, 1237
BlnI CCTAGG 1 cut(s) 1018
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 7 cut(s) 19, 508, 872, 875, 1045, 1235, 1238
Bme1390I CCNGG 1 cut(s) 1246
Bme18I GGWCC 1 cut(s) 656
BmgBI CACGTC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 3 cut(s) 488, 658, 934
BmrFI CCNGG 1 cut(s) 1246
BmsI GCATC 9 cut(s) 112, 236, 380, 455, 541, 769, 894, 1072, 1205
BoxI GACNNNNGTC 1 cut(s) 153
BpiI GAAGAC 2 cut(s) 189, 534
BpmI CTGGAG 3 cut(s) 502, 1095, 1237
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 576
BsaAI YACGTR 2 cut(s) 97, 1162
BsaJI CCNNGG 5 cut(s) 37, 898, 939, 1018, 1245
BsaXI ACNNNNNCTCC 4 cut(s) 475, 505, 924, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 650
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 3 cut(s) 748, 846, 1003
BseBI CCWGG 1 cut(s) 1246
BseDI CCNNGG 5 cut(s) 37, 898, 939, 1018, 1245
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 502, 650
BseMI GCAATG 3 cut(s) 748, 846, 1003
BseMII CTCAG 2 cut(s) 501, 935
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 2 cut(s) 289, 945
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 6 cut(s) 29, 493, 857, 860, 1030, 1245
BseYI CCCAGC 1 cut(s) 174
BshFI GGCC 4 cut(s) 704, 1035, 1223, 1244
BsiHKAI GWGCWC 2 cut(s) 96, 612
BsiSI CCGG 1 cut(s) 1224
BslFI GGGAC 3 cut(s) 1063, 1188, 1262
BslI CCNNNNNNNGG 2 cut(s) 502, 650
BsmAI GTCTC 1 cut(s) 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 3 cut(s) 1063, 1188, 1262
BsnI GGCC 4 cut(s) 704, 1035, 1223, 1244
Bsp1286I GDGCHC 2 cut(s) 96, 612
Bsp1407I TGTACA 1 cut(s) 792
Bsp143I GATC 4 cut(s) 406, 688, 913, 1003
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 3 cut(s) 496, 1085, 1236
BspANI GGCC 4 cut(s) 704, 1035, 1223, 1244
BspCNI CTCAG 2 cut(s) 502, 934
BspLI GGNNCC 3 cut(s) 488, 658, 934
BspMAI CTGCAG 2 cut(s) 19, 1048
BspPI GGATC 3 cut(s) 401, 696, 921
BspQI GCTCTTC 1 cut(s) 600
BspTI CTTAAG 1 cut(s) 661
BsrDI GCAATG 3 cut(s) 748, 846, 1003
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 5 cut(s) 37, 898, 939, 1018, 1245
BssMI GATC 4 cut(s) 406, 688, 913, 1003
BssT1I CCWWGG 2 cut(s) 37, 1018
Bst2UI CCWGG 1 cut(s) 1246
Bst4CI ACNGT 4 cut(s) 355, 631, 792, 940
Bst6I CTCTTC 2 cut(s) 600, 772
BstAFI CTTAAG 1 cut(s) 661
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstAUI TGTACA 1 cut(s) 792
BstBAI YACGTR 2 cut(s) 97, 1162
BstDEI CTNAG 3 cut(s) 510, 706, 921
BstDSI CCRYGG 1 cut(s) 939
BstEII GGTNACC 1 cut(s) 41
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 4 cut(s) 409, 691, 916, 1006
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 4 cut(s) 406, 688, 913, 1003
BstMWI GCNNNNNNNGC 5 cut(s) 64, 306, 882, 1112, 1135
BstNI CCWGG 1 cut(s) 1246
BstNSI RCATGY 3 cut(s) 172, 209, 1154
BstPAI GACNNNNGTC 1 cut(s) 153
BstPI GGTNACC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 1244
BstSFI CTRYAG 2 cut(s) 15, 1044
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 6 cut(s) 29, 493, 857, 860, 1030, 1245
BstV2I GAAGAC 2 cut(s) 189, 534
BstX2I RGATCY 2 cut(s) 406, 913
BstYI RGATCY 2 cut(s) 406, 913
BsuRI GGCC 4 cut(s) 704, 1035, 1223, 1244
BtgI CCRYGG 1 cut(s) 939
BtgZI GCGATG 1 cut(s) 281
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 351
Cfr13I GGNCC 1 cut(s) 656
Csp6I GTAC 4 cut(s) 135, 201, 793, 967
CviAII CATG 6 cut(s) 169, 179, 206, 429, 1143, 1151
CviQI GTAC 4 cut(s) 135, 201, 793, 967
DdeI CTNAG 3 cut(s) 510, 706, 921
DpnI GATC 4 cut(s) 408, 690, 915, 1005
DpnII GATC 4 cut(s) 406, 688, 913, 1003
DraI TTTAAA 1 cut(s) 315
Eam1104I CTCTTC 2 cut(s) 600, 772
EarI CTCTTC 2 cut(s) 600, 772
Eco130I CCWWGG 2 cut(s) 37, 1018
Eco32I GATATC 1 cut(s) 1071
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 2 cut(s) 582, 805
Eco72I CACGTG 1 cut(s) 1162
Eco91I GGTNACC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 1244
EcoRV GATATC 1 cut(s) 1071
EcoT14I CCWWGG 2 cut(s) 37, 1018
EcoT22I ATGCAT 3 cut(s) 448, 806, 1198
ErhI CCWWGG 2 cut(s) 37, 1018
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 6 cut(s) 172, 182, 209, 432, 1146, 1154
FalI AAGNNNNNCTT 6 cut(s) 295, 327, 689, 721, 751, 783
FaqI GGGAC 3 cut(s) 1063, 1188, 1262
FatI CATG 6 cut(s) 168, 178, 205, 428, 1142, 1150
FbaI TGATCA 1 cut(s) 1003
Fnu4HI GCNGC 7 cut(s) 18, 507, 871, 874, 1044, 1234, 1237
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 7 cut(s) 18, 507, 871, 874, 1044, 1234, 1237
FspBI CTAG 7 cut(s) 62, 141, 159, 221, 255, 927, 1019
GluI GCNGC 7 cut(s) 18, 507, 871, 874, 1044, 1234, 1237
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 3 cut(s) 502, 1095, 1237
HaeIII GGCC 4 cut(s) 704, 1035, 1223, 1244
HapII CCGG 1 cut(s) 1224
Hin1II CATG 6 cut(s) 172, 182, 209, 432, 1146, 1154
HincII GTYRAC 1 cut(s) 1174
HindII GTYRAC 1 cut(s) 1174
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 4 cut(s) 358, 518, 944, 1250
HpaII CCGG 1 cut(s) 1224
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 5 cut(s) 94, 166, 203, 1148, 1174
Hpy188I TCNGA 6 cut(s) 363, 601, 625, 778, 902, 924
Hpy188III TCNNGA 6 cut(s) 557, 697, 908, 961, 1007, 1074
Hpy8I GTNNAC 5 cut(s) 94, 166, 203, 1148, 1174
Hpy99I CGWCG 1 cut(s) 967
HpyAV CCTTC 3 cut(s) 783, 829, 976
HpyCH4III ACNGT 4 cut(s) 355, 631, 792, 940
HpyCH4IV ACGT 6 cut(s) 96, 154, 280, 566, 965, 1161
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 306, 882, 1112, 1135
HpyF3I CTNAG 3 cut(s) 510, 706, 921
HpySE526I ACGT 6 cut(s) 96, 154, 280, 566, 965, 1161
Hsp92II CATG 6 cut(s) 172, 182, 209, 432, 1146, 1154
Ksp22I TGATCA 1 cut(s) 1003
Kzo9I GATC 4 cut(s) 406, 688, 913, 1003
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 4 cut(s) 787, 932, 1060, 1135
Lsp1109I GCAGC 6 cut(s) 29, 493, 857, 860, 1030, 1245
LweI GCATC 9 cut(s) 112, 236, 380, 455, 541, 769, 894, 1072, 1205
MaeI CTAG 7 cut(s) 62, 141, 159, 221, 255, 927, 1019
MaeII ACGT 6 cut(s) 96, 154, 280, 566, 965, 1161
MaeIII GTNAC 8 cut(s) 29, 41, 106, 349, 387, 491, 631, 761
MalI GATC 4 cut(s) 408, 690, 915, 1005
MboI GATC 4 cut(s) 406, 688, 913, 1003
MboII GAAGA 7 cut(s) 194, 315, 416, 534, 617, 759, 1091
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 2 cut(s) 406, 913
MhlI GDGCHC 2 cut(s) 96, 612
MlyI GAGTC 2 cut(s) 367, 1244
Mph1103I ATGCAT 3 cut(s) 448, 806, 1198
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 5 cut(s) 248, 314, 662, 675, 951
MspA1I CMGCKG 2 cut(s) 825, 1236
MspCI CTTAAG 1 cut(s) 661
MspI CCGG 1 cut(s) 1224
MspR9I CCNGG 1 cut(s) 1246
MunI CAATTG 1 cut(s) 85
MvaI CCWGG 1 cut(s) 1246
MwoI GCNNNNNNNGC 5 cut(s) 64, 306, 882, 1112, 1135
NdeII GATC 4 cut(s) 406, 688, 913, 1003
NlaIII CATG 6 cut(s) 172, 182, 209, 432, 1146, 1154
NlaIV GGNNCC 3 cut(s) 488, 658, 934
NmuCI GTSAC 5 cut(s) 29, 41, 349, 387, 761
NsiI ATGCAT 3 cut(s) 448, 806, 1198
NspI RCATGY 3 cut(s) 172, 209, 1154
PciI ACATGT 1 cut(s) 1150
PciSI GCTCTTC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 518, 944
PkrI GCNGC 7 cut(s) 19, 508, 872, 875, 1045, 1235, 1238
PleI GAGTC 2 cut(s) 366, 1244
PmaCI CACGTG 1 cut(s) 1162
PmlI CACGTG 1 cut(s) 1162
PpsI GAGTC 2 cut(s) 366, 1244
Ppu21I YACGTR 2 cut(s) 97, 1162
PpuMI RGGWCCY 1 cut(s) 656
PscI ACATGT 1 cut(s) 1150
PshAI GACNNNNGTC 1 cut(s) 153
PshBI ATTAAT 2 cut(s) 675, 951
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 1 cut(s) 1244
PspCI CACGTG 1 cut(s) 1162
PspEI GGTNACC 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 1 cut(s) 1244
PspN4I GGNNCC 3 cut(s) 488, 658, 934
PspPI GGNCC 1 cut(s) 656
PspPPI RGGWCCY 1 cut(s) 656
PstI CTGCAG 2 cut(s) 19, 1048
PsuI RGATCY 2 cut(s) 406, 913
PvuII CAGCTG 1 cut(s) 825
RsaI GTAC 4 cut(s) 136, 202, 794, 968
RsaNI GTAC 4 cut(s) 135, 201, 793, 967
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 5 cut(s) 248, 314, 662, 675, 951
SatI GCNGC 7 cut(s) 18, 507, 871, 874, 1044, 1234, 1237
Sau3AI GATC 4 cut(s) 406, 688, 913, 1003
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 2 cut(s) 367, 1244
ScrFI CCNGG 1 cut(s) 1246
SduI GDGCHC 2 cut(s) 96, 612
SfaNI GCATC 9 cut(s) 112, 236, 380, 455, 541, 769, 894, 1072, 1205
SfcI CTRYAG 2 cut(s) 15, 1044
SinI GGWCC 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 591, 661
SmoI CTYRAG 2 cut(s) 591, 661
SsiI CCGC 3 cut(s) 496, 1085, 1236
SspMI CTAG 7 cut(s) 62, 141, 159, 221, 255, 927, 1019
StyD4I CCNGG 1 cut(s) 1244
StyI CCWWGG 2 cut(s) 37, 1018
TaaI ACNGT 4 cut(s) 355, 631, 792, 940
TaiI ACGT 6 cut(s) 99, 157, 283, 569, 968, 1164
TaqI TCGA 1 cut(s) 907
TatI WGTACW 3 cut(s) 134, 200, 792
TauI GCSGC 1 cut(s) 1239
TfiI GAWTC 2 cut(s) 518, 944
Tru1I TTAA 5 cut(s) 248, 314, 662, 675, 951
Tru9I TTAA 5 cut(s) 248, 314, 662, 675, 951
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 5 cut(s) 29, 41, 349, 387, 761
TseI GCWGC 6 cut(s) 17, 506, 870, 873, 1043, 1233
Tsp45I GTSAC 5 cut(s) 29, 41, 349, 387, 761
TspDTI ATGAA 5 cut(s) 117, 195, 225, 618, 838
TspGWI ACGGA 2 cut(s) 325, 479
TspRI CASTG 1 cut(s) 358
Vha464I CTTAAG 1 cut(s) 661
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 656
VspI ATTAAT 2 cut(s) 675, 951
XapI RAATTY 4 cut(s) 250, 259, 316, 971
XceI RCATGY 3 cut(s) 172, 209, 1154
XmaJI CCTAGG 1 cut(s) 1018
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 7 cut(s) 62, 141, 159, 221, 255, 927, 1019
Zsp2I ATGCAT 3 cut(s) 448, 806, 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.