Rroxscaffold_2G00107090

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005617
Physical Location & Seq
Reverse (-)
30810263 .. 30815307
5045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00107090.1

Sequence Viewer

Length: 1275 bp
ATGGATGTTCCCTCTCCAGAACCACATAGCGACTCTCGAAGTGACCAATATCCTTTGCTACTAGAGCAGATGGAAAGCCCTAACAGTCATGTGCACATAATTGACGTAACGAGGAATGATGATACCTCATCAAGTTCATCTAATGATGATCAACTTCCTAGAGTGGACTTGCCTCAGCATGAAGATAGACTGTCACGTATTACACATGCCCCCACTTATCAGGCTGCTTCAACTTCGTCAAACAGATTAAGTGCTAGGAGTTCATCATTTATGAGGCGGGGTGATGGGTATAGTCGCCGCAGAAGAAGCCCATTGAATTCAGGGCTGTGGATTTCTGTGGAACTTCTTGTCACTATGAGTCAGATAATAGCATCTATCGTTGTTTTGTCATTGTCGACAAATGAAAATCCGCAAGCCCCTTTGTTCGCATGGATTGTGGGCTATGCATCTGGCTGTGTTGCAACACTACCTATTCTTTACTGGAGATTCCGTAACCGCAACAGGGGTGCTGAGCTAGAATCCACTCAATCTCATCAAGGGTCTTCTGAACGCAATCCTCCTGAACCAACTTCTTATACAGCTATTTCAATTACTCCAGCTTCAGACGAAGAAAGAAATCACACTACAGAAACAGTCATGAGAAACCCTCAAGTTGCAGAACCCTTAAGTGCAAGACTTAATGGGTTGGTGGATCATTTCAAGATGGCATTAGATTGTTTCTTCGCAGTCTGGTTTGTTGTGGGAAATGTGTGGATTTTTGGAGGTCACTCTTCTCCCTCTGATGCTCCTAAATTGTATAGGTTATGCATAGTGTTCCTCACCTTTAGCTGTATCGGATATGCCATGCCATTCATACTCTGTGCAACAATTTGTTGCTGCCTGCCGTGCATCATTTCTGTCCTGGGATTCCGAGAGGACCATGCTCAGACTAGAGGAGCCACCACAGAGTCAATTAATGCTCTGCCAACCTACAAATTCAGGCTGAAGAGAAATGGGATGAATGATGATCAGGAGAGTAACACCTCTGGTGAAGGAGGACTCTTGGCTGCAGGGACAGAGAAGGAGCGTGCAATATCAGAAGAAGATGCGGTTTGTAGTATTTGCCTGGCAAATTATTCAGATAATGATGAGGTAAGAGAATTAACATGTTCACATGTCTTTCATGTGGAGTGTGTTGACAAATGGTTGAAAATCAATGCCTCATGTCCCCTCTGTAAGTCGGAGGTTGGAGAGAGCAGCGGTACCACGCCGGGCAGAGACTCCAGCCAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

424

Amino Acids

46.88

Weight (kDa)

5.42

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 362 - 406 1.4e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 365 - 406 1.2e-14 Ring finger domain
zf-RING_11 PF17123 365 - 392 2.4e-09 RING-like zinc finger
zf-C3HC4 PF00097 365 - 405 6.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 365 - 405 1.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 365 - 406 3.9e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1241
AccB1I GGYRCC 1 cut(s) 1241
AccI GTMKAC 1 cut(s) 395
AciI CCGC 6 cut(s) 277, 298, 410, 496, 1088, 1239
AclWI GGATC 1 cut(s) 699
AcsI RAATTY 2 cut(s) 316, 974
AcuI CTGAAG 2 cut(s) 585, 1004
AfaI GTAC 1 cut(s) 1243
AfiI CCNNNNNNNGG 1 cut(s) 502
AflII CTTAAG 1 cut(s) 664
AflIII ACRYGT 2 cut(s) 1145, 1153
AgsI TTSAA 5 cut(s) 231, 316, 588, 700, 1189
AjnI CCWGG 2 cut(s) 900, 1104
AluBI AGCT 4 cut(s) 514, 581, 599, 828
AluI AGCT 4 cut(s) 514, 581, 599, 828
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 1251
Alw44I GTGCAC 1 cut(s) 92
AlwI GGATC 1 cut(s) 699
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 4 cut(s) 224, 876, 1046, 1236
ApoI RAATTY 2 cut(s) 316, 974
AseI ATTAAT 1 cut(s) 954
Asp718I GGTACC 1 cut(s) 1241
AspS9I GGNCC 1 cut(s) 916
AsuC2I CCSGG 1 cut(s) 1251
AsuHPI GGTGA 3 cut(s) 293, 811, 1040
AvaII GGWCC 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 96
BanI GGYRCC 1 cut(s) 1241
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 4 cut(s) 211, 863, 1033, 1248
BccI CCATC 3 cut(s) 64, 278, 697
BceAI ACGGC 1 cut(s) 868
BciT130I CCWGG 2 cut(s) 902, 1106
BclI TGATCA 2 cut(s) 148, 1006
BcnI CCSGG 1 cut(s) 1251
BcoDI GTCTC 1 cut(s) 1251
BfaI CTAG 5 cut(s) 62, 159, 255, 515, 930
BfmI CTRYAG 2 cut(s) 624, 1047
BfrI CTTAAG 1 cut(s) 664
BisI GCNGC 5 cut(s) 225, 298, 877, 1047, 1237
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 5 cut(s) 226, 299, 878, 1048, 1238
Bme1390I CCNGG 3 cut(s) 902, 1106, 1251
Bme18I GGWCC 1 cut(s) 916
BmgT120I GGNCC 1 cut(s) 916
BmiI GGNNCC 2 cut(s) 937, 1243
BmrFI CCNGG 3 cut(s) 902, 1106, 1251
BmsI GCATC 5 cut(s) 380, 455, 772, 897, 1075
BpiI GAAGAC 1 cut(s) 534
BplI GAGNNNNNCTC 2 cut(s) 631, 663
BpmI CTGGAG 3 cut(s) 502, 579, 1246
Bpu10I CCTNAGC 1 cut(s) 174
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 633
BpuMI CCSGG 1 cut(s) 1251
BsaAI YACGTR 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 901
BsaXI ACNNNNNCTCC 2 cut(s) 475, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 485
BseBI CCWGG 2 cut(s) 902, 1106
BseDI CCNNGG 1 cut(s) 901
BseGI GGATG 2 cut(s) 10, 1002
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMII CTCAG 3 cut(s) 188, 501, 938
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 948
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 4 cut(s) 211, 863, 1033, 1248
BshNI GGYRCC 1 cut(s) 1241
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 1250
BslFI GGGAC 2 cut(s) 1066, 1191
BslI CCNNNNNNNGG 1 cut(s) 502
BsmAI GTCTC 1 cut(s) 1251
BsmFI GGGAC 2 cut(s) 1066, 1191
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 148, 691, 1006
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 6 cut(s) 277, 298, 410, 496, 1088, 1239
BspCNI CTCAG 3 cut(s) 187, 502, 937
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 2 cut(s) 937, 1243
BspMAI CTGCAG 1 cut(s) 1051
BspPI GGATC 1 cut(s) 699
BspT107I GGYRCC 1 cut(s) 1241
BspTI CTTAAG 1 cut(s) 664
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 901
BssMI GATC 3 cut(s) 148, 691, 1006
Bst2UI CCWGG 2 cut(s) 902, 1106
Bst4CI ACNGT 3 cut(s) 86, 192, 634
Bst6I CTCTTC 2 cut(s) 775, 980
BstAFI CTTAAG 1 cut(s) 664
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 4 cut(s) 414, 881, 1068, 1268
BstDEI CTNAG 3 cut(s) 174, 510, 924
BstF5I GGATG 2 cut(s) 10, 1002
BstKTI GATC 3 cut(s) 151, 694, 1009
BstMAI GTCTC 1 cut(s) 1251
BstMBI GATC 3 cut(s) 148, 691, 1006
BstMWI GCNNNNNNNGC 3 cut(s) 64, 306, 885
BstNI CCWGG 2 cut(s) 902, 1106
BstNSI RCATGY 3 cut(s) 209, 1149, 1157
BstSCI CCNGG 3 cut(s) 900, 1104, 1249
BstSFI CTRYAG 2 cut(s) 624, 1047
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 211, 863, 1033, 1248
BstV2I GAAGAC 1 cut(s) 534
BtsCI GGATG 2 cut(s) 10, 1002
Cac8I GCNNGC 4 cut(s) 414, 881, 1068, 1268
CciI TCATGA 1 cut(s) 636
Cfr13I GGNCC 1 cut(s) 916
Csp6I GTAC 1 cut(s) 1242
CviQI GTAC 1 cut(s) 1242
DdeI CTNAG 3 cut(s) 174, 510, 924
DpnI GATC 3 cut(s) 150, 693, 1008
DpnII GATC 3 cut(s) 148, 691, 1006
Eam1104I CTCTTC 2 cut(s) 775, 980
EarI CTCTTC 2 cut(s) 775, 980
Eco47I GGWCC 1 cut(s) 916
Eco57I CTGAAG 2 cut(s) 585, 1004
EcoRI GAATTC 1 cut(s) 316
EcoRII CCWGG 2 cut(s) 900, 1104
EcoT22I ATGCAT 2 cut(s) 448, 809
FaqI GGGAC 2 cut(s) 1066, 1191
FauI CCCGC 1 cut(s) 270
FbaI TGATCA 2 cut(s) 148, 1006
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 5 cut(s) 225, 298, 877, 1047, 1237
FokI GGATG 2 cut(s) 17, 1009
Fsp4HI GCNGC 5 cut(s) 225, 298, 877, 1047, 1237
FspBI CTAG 5 cut(s) 62, 159, 255, 515, 930
GluI GCNGC 5 cut(s) 225, 298, 877, 1047, 1237
GsuI CTGGAG 3 cut(s) 502, 579, 1246
HapII CCGG 1 cut(s) 1250
HincII GTYRAC 2 cut(s) 396, 1177
HindII GTYRAC 2 cut(s) 396, 1177
HinfI GANTC 8 cut(s) 32, 358, 486, 518, 906, 947, 1038, 1259
HpaII CCGG 1 cut(s) 1250
HphI GGTGA 3 cut(s) 293, 811, 1040
Hpy166II GTNNAC 5 cut(s) 94, 166, 396, 1151, 1177
Hpy188III TCNNGA 6 cut(s) 17, 36, 560, 637, 700, 1010
Hpy8I GTNNAC 5 cut(s) 94, 166, 396, 1151, 1177
HpyAV CCTTC 2 cut(s) 1025, 1054
HpyCH4III ACNGT 3 cut(s) 86, 192, 634
HpyCH4IV ACGT 2 cut(s) 105, 196
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 306, 885
HpyF3I CTNAG 3 cut(s) 174, 510, 924
HpySE526I ACGT 2 cut(s) 105, 196
KpnI GGTACC 1 cut(s) 1245
Ksp22I TGATCA 2 cut(s) 148, 1006
Kzo9I GATC 3 cut(s) 148, 691, 1006
LmnI GCTCC 3 cut(s) 790, 935, 1063
Lsp1109I GCAGC 4 cut(s) 211, 863, 1033, 1248
LweI GCATC 5 cut(s) 380, 455, 772, 897, 1075
MaeI CTAG 5 cut(s) 62, 159, 255, 515, 930
MaeII ACGT 2 cut(s) 105, 196
MaeIII GTNAC 7 cut(s) 41, 106, 192, 349, 491, 764, 1016
MalI GATC 3 cut(s) 150, 693, 1008
MboI GATC 3 cut(s) 148, 691, 1006
MboII GAAGA 9 cut(s) 194, 315, 534, 620, 712, 762, 997, 1091, 1094
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 9 cut(s) 99, 316, 588, 791, 867, 951, 974, 1111, 1139
MlyI GAGTC 5 cut(s) 26, 367, 956, 1032, 1253
MmeI TCCRAC 2 cut(s) 1200, 1207
Mph1103I ATGCAT 2 cut(s) 448, 809
MseI TTAA 5 cut(s) 248, 665, 678, 954, 1142
MspA1I CMGCKG 1 cut(s) 1239
MspCI CTTAAG 1 cut(s) 664
MspI CCGG 1 cut(s) 1250
MspR9I CCNGG 3 cut(s) 902, 1106, 1251
MvaI CCWGG 2 cut(s) 902, 1106
MwoI GCNNNNNNNGC 3 cut(s) 64, 306, 885
NciI CCSGG 1 cut(s) 1251
NdeII GATC 3 cut(s) 148, 691, 1006
NlaIV GGNNCC 2 cut(s) 937, 1243
NmuCI GTSAC 4 cut(s) 41, 192, 349, 764
NsiI ATGCAT 2 cut(s) 448, 809
NspI RCATGY 3 cut(s) 209, 1149, 1157
PagI TCATGA 1 cut(s) 636
PciI ACATGT 2 cut(s) 1145, 1153
PfeI GAWTC 3 cut(s) 486, 518, 906
PkrI GCNGC 5 cut(s) 226, 299, 878, 1048, 1238
PleI GAGTC 5 cut(s) 26, 366, 955, 1032, 1253
PpsI GAGTC 5 cut(s) 26, 366, 955, 1032, 1253
Ppu21I YACGTR 1 cut(s) 197
PscI ACATGT 2 cut(s) 1145, 1153
PshBI ATTAAT 1 cut(s) 954
Psp6I CCWGG 2 cut(s) 900, 1104
PspGI CCWGG 2 cut(s) 900, 1104
PspN4I GGNNCC 2 cut(s) 937, 1243
PspPI GGNCC 1 cut(s) 916
PstI CTGCAG 1 cut(s) 1051
RsaI GTAC 1 cut(s) 1243
RsaNI GTAC 1 cut(s) 1242
SalI GTCGAC 1 cut(s) 394
SaqAI TTAA 5 cut(s) 248, 665, 678, 954, 1142
SatI GCNGC 5 cut(s) 225, 298, 877, 1047, 1237
Sau3AI GATC 3 cut(s) 148, 691, 1006
Sau96I GGNCC 1 cut(s) 916
SchI GAGTC 5 cut(s) 26, 367, 956, 1032, 1253
ScrFI CCNGG 3 cut(s) 902, 1106, 1251
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 5 cut(s) 380, 455, 772, 897, 1075
SfcI CTRYAG 2 cut(s) 624, 1047
SinI GGWCC 1 cut(s) 916
SmlI CTYRAG 2 cut(s) 648, 664
SmoI CTYRAG 2 cut(s) 648, 664
Sse9I AATT 9 cut(s) 99, 316, 588, 791, 867, 951, 974, 1111, 1139
SsiI CCGC 6 cut(s) 277, 298, 410, 496, 1088, 1239
SspMI CTAG 5 cut(s) 62, 159, 255, 515, 930
StyD4I CCNGG 3 cut(s) 900, 1104, 1249
TaaI ACNGT 3 cut(s) 86, 192, 634
TaiI ACGT 2 cut(s) 108, 199
TaqI TCGA 2 cut(s) 37, 395
TasI AATT 9 cut(s) 99, 316, 588, 791, 867, 951, 974, 1111, 1139
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 3 cut(s) 486, 518, 906
Tru1I TTAA 5 cut(s) 248, 665, 678, 954, 1142
Tru9I TTAA 5 cut(s) 248, 665, 678, 954, 1142
TseFI GTSAC 4 cut(s) 41, 192, 349, 764
TseI GCWGC 4 cut(s) 224, 876, 1046, 1236
Tsp45I GTSAC 4 cut(s) 41, 192, 349, 764
TspDTI ATGAA 7 cut(s) 126, 195, 252, 417, 841, 1013, 1151
TspGWI ACGGA 1 cut(s) 479
Vha464I CTTAAG 1 cut(s) 664
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 916
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 2 cut(s) 316, 974
XceI RCATGY 3 cut(s) 209, 1149, 1157
XmiI GTMKAC 1 cut(s) 395
XspI CTAG 5 cut(s) 62, 159, 255, 515, 930
Zsp2I ATGCAT 2 cut(s) 448, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.