Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005640

Physical Location & Seq

Genomic Coordinates Forward (+)

1474679 .. 1480586

Gene Structure 5908 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G04270.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2991 bp

ATGGATGTTACTGAAGTTCCATGGCGTCGTCTTCCTCAATTTTCCGTTTCTTCACGCGCCTCGTGGCTCGTTTCTTCTGGGTTTCCGCTTTCTTCATATATGTTCTCTCATGTTGAGCGTGGAAAGACGTTTAGGCTTACCTTGTGCTTTGGGGTCTCTCGTTTACGTCCAAGGTCTGCTATTGTAAGTGCACAACAAGAACAGCCACCTAGTCGTCTCAAAGGATTATGCGAGGTCGTTTGGATTGTGGAAGCTGATCTGGCAGCCAATGAACATCTCTACGTTACTGGAGATCCTTCTACATTAGGTAGCTGGGAGCCAGACTGCGCAATCTCAATGTATCCTACGGAAAATGATAATGAATGGGAAGCTAAAGTCAAGATTGCTTCTGGTGTAAATTTCAGGTATAATTATCTGTTAAAAGCTGGATATGGATCTTCATCGGATGTCATCTGGAGACCAGGGCCACAATTTTCGTTATCAGTTCCTTCCTCTGTCAATCAAGACAGAAAAATAATTATAAGGGATTCATGGATGAGTATGAGTATTTCCTCAAAATCACAAGAATCCTACGGATGGGGGTCTTGGATTGATGATGCTTACCTTTTTCCAAACTGTGTTACCCCAGCTCAAAGCGAAGATGAATGCACTTCCGCCGATAGTGCTATAGAAGTCCCGAGAACACATCTGAATGATAAGCAAGTAGGAGCTGAATCTTTCTTATGTGATGAGCTTGCAGCCTTTTCGTCAGAGAACTCAAACTTAAGTGCGTTATTTTCTGACAACTATCAGCCTATCGAGGAACCGTGGTTGATTCAAGAATCTATTACCTTACAACATGAAAGGAATATGCAGACTGATAGCGAACAAGATGTTGAAAGCTGTGATGACAATGAAAATAACTTAAATACCGATGAACAAAATCACCAGCTGACTGAGACTCTCTTACCAGATGGTGGATTTTTCCAATCAGAATCCATTGCTACTACGATACTGATCAATTCTTCTATATGTACGGTGCAAAGGATTGCTGTATTGGAAGGTGGAAAGCTGGTCGAGTTATTGTTGGAACCAGTTAAGACCAATGTGCAGTGTGATAGTGTTTACCTGGGCGTAATCACTAAGTTTGTGCCCCATATGGGTGGTGCATTTGTGAATATCGGAAGTGCTAGACATTCTTTCATGGACATTAAGTCAAACAGGGAACCATTCATATTCCCTCCATTTTGTGATGGATCGAAGAAGCAAGCAGCTGATGGTTCTCCGATCCTCTCTATGAATGACATTCCAGCTCCCCATGAAATCGAACATGCTTCCTATGACTTTGAAGCCAGTAGTTTATTAGACATTGATTCAAATGACCCTGGGGAATCTTTTCATGATGATGACGATGAGCATGAAAATGATGAATATCATGTCTCAGATCATCTAGCTGGACTAGTTAATGGAACTGTAGTTAACCATGGCGCTGTGGAAGTTGGATCTGAGAATGGCCATATTCCTATGGAGAGAGGACACTCTGCAGATTCTCTTGATTCGAACGCATCAGTTGCAAAAGCTTCTAAGGTGATGTCGTCTAAGGATAATAAGTGGATTCAAGTTCGGAAAGGAACCAAAATTATTGTGCAAGTTGTTAAAGAGGGCCTCGGTACGAAAGGTCCAACTCTTACTGCTTACCCGAAGCTGAGAAGTAGATTCTGGGTACTATTGACTCGTTGCAAACGAATTGGAGTCTCGAAAAAAATTTCGGGAGTTGAGAGAACCCGGTTAAAAGTTATAGCAAAAACATTGCAGCCTCAAGGTTTTGGCCTGACTGTAAGGACTGTAGCTGCTGGCCATTCTCTAGAAGAACTGCAGAAAGACTTAGATGGTCTGCTTTTAACCTGGAAGAACATTACAGATGAAGCAAAATCTGCAGCCCTTGCTGCAGATGAAGGTGTGGAAGGAGCCATTCCAGCGCTGCTACATAGAGCAATGGGTCAAACACTATCAGTTGTGCAGGACTATTTCAATGACAAGGTTGAGAAGATGGTGGTTGACTCTCCCAGGACATACCATGAGGTCACACACTACCTGCAGGATATGGCACCTGATCTTTGTAATCGAGTGGAATTGCATGATAAGGGTATCCCTCTTTTTGATTTATATGAAATTGAGGAGGAAATCGAAGGTATTCTTAGTAAAAGAGTTCCACTTTCCAATGGAGGTTCTTTGGTGATTGAACAAACTGAGGCGTTAGTCTCTATTGATGTGAACGGAGGACATGGAATGTTTGGTCAGGGTAATTCACAGGAGAAAGCTATTTTAGAAGTTAATCTTGCTGCTGCCAGACAAATTGCAAGGGAGATTCGGCTGAGAGATATTGGCGGTATCATTGTGGTAGATTTCATCGACATGGCAGATGAATCAAATAAGAGACTCGTTTATGAAGAGGTTAAGAAAGCCGTGGAGAGAGATAGGTCACTGGTAAAAGTATCAGAGCTGTCTAGACACGGACTTATGGAGATAACAAGAAAAAGGGTTCGGCCGAGTGTAACATTTATGATCAGTGAACCATGTTCTTGCTGTCACGCAACTGGTAGAGTGGAGGCACTGGAGACTACCTTTTCCAAAATTGAACAAGAGATTTGTCGGCAGCTTGCAAAGATGGAGAAAAGAGGAGACCTGGAAAACCCCAAATCGTGGCCAAGATTCATATTGCGGGTGGACAGTCATATGTCAAGCTTCTTGACTACGGGGAAGAGGACGCGGCTTGCAATCCTCAGTAGTTCCCTGAAAGTATGGATTCTCTTAAAGGTTGCTAGACATTTCACCAGAGGAACCTTTGAGGTTAAACCGTTCATGGATGAGAAGACAGTAAACGAAAGACAGCATCAAGTTGCCATTTCACTGCTCAAGAAAGCTGACGCCATAGCAGACTCCTCCGGCAAGAAGAAGCTCACTCTAATTCCAATTAAGAAGGAGAAGACCAGTGGTAAACAAAGGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010239 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0031425 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 RNase_E_G

Protein Analysis

996

Amino Acids

110.9

Weight (kDa)

5.59

Isoelectric Point (pI)

47.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	84 - 175	9.4e-18	Starch binding domain
RNase_E_G	PF10150	567 - 846	1.6e-86	Ribonuclease E/G family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270
fragaria_vesca	FvH4_6g40410
malus_domestica	MD09G1130200.v1.1 MD17G1105400.v1.1
prunus_persica	Prupe.3G194900_v2.0.a1 Prupe.3G194900_v2.0.a1
pyrus_communis	pycom09g05330 pycom17g10950 pycom17g10970
rosa_chinensis	RchiOBHm_Chr2g0155641
rosa_laevigata	RLG00000020850
rosa_multiflora	Rmu_ssc0000430.1_g000006
rosa_roxburghii	Rroxscaffold_2G00093600
rosa_rugosa	Rorug02G0448700 Rorug02G0448700
rosa_samantha	Rh2AG514800 Rh2BG525700 Rh2CG499700 Rh2DG534600
rosa_wichuraiana	Rw2G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	521
Acc16I	TGCGCA	1 cut(s)	328
Acc36I	ACCTGC	1 cut(s)	2082
AccB1I	GGYRCC	1 cut(s)	2086
AccB7I	CCANNNNNTGG	2 cut(s)	956, 2682
AccII	CGCG	2 cut(s)	57, 2749
AciI	CCGC	5 cut(s)	86, 654, 2367, 2701, 2749
AclWI	GGATC	5 cut(s)	287, 442, 1243, 1261, 1489
AcoI	YGGCCR	4 cut(s)	1492, 1834, 2525, 2684
AcsI	RAATTY	2 cut(s)	397, 1743
AcuI	CTGAAG	1 cut(s)	33
AcyI	GRCGYC	2 cut(s)	25, 2907
AfaI	GTAC	3 cut(s)	1015, 1651, 1704
AfeI	AGCGCT	1 cut(s)	1959
AfiI	CCNNNNNNNGG	4 cut(s)	576, 956, 1139, 2682
AflII	CTTAAG	1 cut(s)	763
AgsI	TTSAA	8 cut(s)	818, 878, 1328, 1356, 1598, 2011, 2222, 2618
AhdI	GACNNNNNGTC	1 cut(s)	1192
AhlI	ACTAGT	1 cut(s)	1438
AjnI	CCWGG	6 cut(s)	460, 1107, 1363, 1883, 2045, 2664
Alw21I	GWGCWC	1 cut(s)	193
Alw44I	GTGCAC	1 cut(s)	189
AlwI	GGATC	5 cut(s)	287, 442, 1243, 1261, 1489
Ama87I	CYCGRG	1 cut(s)	676
Aor51HI	AGCGCT	1 cut(s)	1959
AoxI	GGCC	7 cut(s)	464, 1492, 1642, 1807, 1834, 2525, 2684
ApaLI	GTGCAC	1 cut(s)	189
ApoI	RAATTY	2 cut(s)	397, 1743
Asp700I	GAANNNNTTC	2 cut(s)	1374, 2172
AspLEI	GCGC	4 cut(s)	59, 329, 1469, 1960
AspS9I	GGNCC	3 cut(s)	464, 1642, 1658
AsuC2I	CCSGG	1 cut(s)	1765
AsuHPI	GGTGA	4 cut(s)	917, 1579, 2227, 2803
AsuII	TTCGAA	1 cut(s)	1538
AvaI	CYCGRG	1 cut(s)	676
AvaII	GGWCC	1 cut(s)	1658
BaeGI	GKGCMC	2 cut(s)	193, 1134
BalI	TGGCCA	3 cut(s)	1494, 1836, 2686
BanI	GGYRCC	1 cut(s)	2086
BauI	CACGAG	1 cut(s)	61
BbsI	GAAGAC	3 cut(s)	23, 2858, 2972
Bbv12I	GWGCWC	1 cut(s)	193
BccI	CCATC	7 cut(s)	570, 947, 1226, 1250, 1862, 2023, 2641
BceAI	ACGGC	1 cut(s)	2429
BcgI	CGANNNNNNTGC	2 cut(s)	726, 760
BciT130I	CCWGG	6 cut(s)	462, 1109, 1365, 1885, 2047, 2666
BciVI	GTATCC	2 cut(s)	351, 2138
BclI	TGATCA	2 cut(s)	996, 2544
BcnI	CCSGG	1 cut(s)	1765
BcuI	ACTAGT	1 cut(s)	1438
BfaI	CTAG	7 cut(s)	210, 1170, 1430, 1439, 1844, 2487, 2802
BfmI	CTRYAG	8 cut(s)	666, 1452, 1521, 1824, 1853, 1914, 1926, 2075
BfoI	RGCGCY	2 cut(s)	1470, 1961
BfrI	CTTAAG	1 cut(s)	763
BfuAI	ACCTGC	1 cut(s)	2082
BfuI	GTATCC	2 cut(s)	351, 2138
Bme1390I	CCNGG	7 cut(s)	462, 1109, 1365, 1765, 1885, 2047, 2666
Bme18I	GGWCC	1 cut(s)	1658
BmeRI	GACNNNNNGTC	1 cut(s)	1192
BmeT110I	CYCGRG	1 cut(s)	676
BmgT120I	GGNCC	3 cut(s)	464, 1642, 1658
BmiI	GGNNCC	8 cut(s)	318, 804, 1071, 1206, 1612, 1948, 2088, 2821
BmrFI	CCNGG	7 cut(s)	462, 1109, 1365, 1765, 1885, 2047, 2666
BmsI	GCATC	3 cut(s)	586, 1553, 2881
BpiI	GAAGAC	3 cut(s)	23, 2858, 2972
BpmI	CTGGAG	3 cut(s)	309, 475, 2615
Bpu14I	TTCGAA	1 cut(s)	1538
BpuEI	CTTGAG	2 cut(s)	1782, 2879
BpuMI	CCSGG	1 cut(s)	1765
BsaBI	GATNNNNATC	4 cut(s)	433, 439, 995, 1410
BsaHI	GRCGYC	2 cut(s)	25, 2907
BsaI	GGTCTC	3 cut(s)	160, 451, 2655
Bsc4I	CCNNNNNNNGG	4 cut(s)	576, 956, 1139, 2682
Bse1I	ACTGG	7 cut(s)	292, 1073, 1332, 2469, 2581, 2598, 2970
Bse3DI	GCAATG	3 cut(s)	978, 1787, 1980
Bse8I	GATNNNNATC	4 cut(s)	433, 439, 995, 1410
BseBI	CCWGG	6 cut(s)	462, 1109, 1365, 1885, 2047, 2666
BseGI	GGATG	5 cut(s)	10, 451, 540, 581, 2851
BseJI	GATNNNNATC	4 cut(s)	433, 439, 995, 1410
BseLI	CCNNNNNNNGG	4 cut(s)	576, 956, 1139, 2682
BseMI	GCAATG	3 cut(s)	978, 1787, 1980
BseMII	CTCAG	7 cut(s)	927, 1434, 1476, 1676, 2220, 2345, 2776
BseNI	ACTGG	7 cut(s)	292, 1073, 1332, 2469, 2581, 2598, 2970
BseRI	GAGGAG	3 cut(s)	2171, 2673, 2911
BseSI	GKGCMC	2 cut(s)	193, 1134
BseX3I	CGGCCG	1 cut(s)	2525
BseYI	CCCAGC	2 cut(s)	312, 625
BsgI	GTGCAG	2 cut(s)	1109, 2018
Bsh1236I	CGCG	2 cut(s)	57, 2749
Bsh1285I	CGRYCG	1 cut(s)	2528
BshFI	GGCC	7 cut(s)	466, 1494, 1644, 1809, 1836, 2527, 2686
BshNI	GGYRCC	1 cut(s)	2086
BsiEI	CGRYCG	1 cut(s)	2528
BsiHKAI	GWGCWC	1 cut(s)	193
BsiHKCI	CYCGRG	1 cut(s)	676
BsiSI	CCGG	2 cut(s)	1765, 2925
BslFI	GGGAC	1 cut(s)	659
BslI	CCNNNNNNNGG	4 cut(s)	576, 956, 1139, 2682
BsmBI	CGTCTC	1 cut(s)	221
BsmFI	GGGAC	1 cut(s)	659
BsmI	GAATGC	1 cut(s)	650
BsnI	GGCC	7 cut(s)	466, 1494, 1644, 1809, 1836, 2527, 2686
Bso31I	GGTCTC	3 cut(s)	160, 451, 2655
BsoBI	CYCGRG	1 cut(s)	676
Bsp119I	TTCGAA	1 cut(s)	1538
Bsp1286I	GDGCHC	2 cut(s)	193, 1134
Bsp19I	CCATGG	2 cut(s)	20, 1462
BspACI	CCGC	5 cut(s)	86, 654, 2367, 2701, 2749
BspANI	GGCC	7 cut(s)	466, 1494, 1644, 1809, 1836, 2527, 2686
BspCNI	CTCAG	7 cut(s)	928, 1433, 1477, 1677, 2221, 2346, 2775
BspFNI	CGCG	2 cut(s)	57, 2749
BspHI	TCATGA	1 cut(s)	1378
BspLI	GGNNCC	8 cut(s)	318, 804, 1071, 1206, 1612, 1948, 2088, 2821
BspMAI	CTGCAG	5 cut(s)	1525, 1857, 1918, 1930, 2079
BspMI	ACCTGC	1 cut(s)	2082
BspPI	GGATC	5 cut(s)	287, 442, 1243, 1261, 1489
BspT104I	TTCGAA	1 cut(s)	1538
BspT107I	GGYRCC	1 cut(s)	2086
BspTI	CTTAAG	1 cut(s)	763
BspTNI	GGTCTC	3 cut(s)	160, 451, 2655
BsrDI	GCAATG	3 cut(s)	978, 1787, 1980
BsrI	ACTGG	7 cut(s)	292, 1073, 1332, 2469, 2581, 2598, 2970
BssNI	GRCGYC	2 cut(s)	25, 2907
BssSI	CACGAG	1 cut(s)	61
BssT1I	CCWWGG	3 cut(s)	20, 170, 1462
Bst2BI	CACGAG	1 cut(s)	61
Bst2UI	CCWGG	6 cut(s)	462, 1109, 1365, 1885, 2047, 2666
Bst4CI	ACNGT	9 cut(s)	617, 807, 1018, 1453, 1816, 1825, 2711, 2838, 2857
Bst6I	CTCTTC	2 cut(s)	2424, 2735
BstACI	GRCGYC	2 cut(s)	25, 2907
BstAFI	CTTAAG	1 cut(s)	763
BstAPI	GCANNNNNTGC	3 cut(s)	1550, 1913, 1922
BstBI	TTCGAA	1 cut(s)	1538
BstC8I	GCNNGC	5 cut(s)	735, 1248, 1834, 2640, 2754
BstDSI	CCRYGG	4 cut(s)	20, 806, 1462, 2445
BstF5I	GGATG	5 cut(s)	10, 451, 540, 581, 2851
BstFNI	CGCG	2 cut(s)	57, 2749
BstH2I	RGCGCY	2 cut(s)	1470, 1961
BstHHI	GCGC	4 cut(s)	59, 329, 1469, 1960
BstMCI	CGRYCG	1 cut(s)	2528
BstMWI	GCNNNNNNNGC	7 cut(s)	260, 662, 1550, 1913, 1922, 1925, 1955
BstNI	CCWGG	6 cut(s)	462, 1109, 1365, 1885, 2047, 2666
BstNSI	RCATGY	1 cut(s)	1313
BstSCI	CCNGG	7 cut(s)	460, 1107, 1363, 1763, 1883, 2045, 2664
BstSFI	CTRYAG	8 cut(s)	666, 1452, 1521, 1824, 1853, 1914, 1926, 2075
BstSLI	GKGCMC	2 cut(s)	193, 1134
BstUI	CGCG	2 cut(s)	57, 2749
BstV2I	GAAGAC	3 cut(s)	23, 2858, 2972
BstX2I	RGATCY	3 cut(s)	292, 434, 1481
BstXI	CCANNNNNNTGG	1 cut(s)	1142
BstYI	RGATCY	3 cut(s)	292, 434, 1481
BstZI	CGGCCG	1 cut(s)	2525
BsuI	GTATCC	2 cut(s)	351, 2138
BsuRI	GGCC	7 cut(s)	466, 1494, 1644, 1809, 1836, 2527, 2686
BtgI	CCRYGG	4 cut(s)	20, 806, 1462, 2445
BtsCI	GGATG	5 cut(s)	10, 451, 540, 581, 2851
BtsI	GCAGTG	2 cut(s)	1097, 2888
BtsIMutI	CAGTG	6 cut(s)	1097, 2462, 2554, 2591, 2888, 2977
BveI	ACCTGC	1 cut(s)	2082
Cac8I	GCNNGC	5 cut(s)	735, 1248, 1834, 2640, 2754
CciI	TCATGA	1 cut(s)	1378
CfoI	GCGC	4 cut(s)	59, 329, 1469, 1960
Cfr13I	GGNCC	3 cut(s)	464, 1642, 1658
CseI	GACGC	3 cut(s)	14, 2755, 2915
Csp6I	GTAC	3 cut(s)	1014, 1650, 1703
CviQI	GTAC	3 cut(s)	1014, 1650, 1703
DriI	GACNNNNNGTC	1 cut(s)	1192
EaeI	YGGCCR	4 cut(s)	1492, 1834, 2525, 2684
EagI	CGGCCG	1 cut(s)	2525
Eam1104I	CTCTTC	2 cut(s)	2424, 2735
Eam1105I	GACNNNNNGTC	1 cut(s)	1192
EarI	CTCTTC	2 cut(s)	2424, 2735
EciI	GGCGGA	1 cut(s)	643
EclXI	CGGCCG	1 cut(s)	2525
Eco130I	CCWWGG	3 cut(s)	20, 170, 1462
Eco31I	GGTCTC	3 cut(s)	160, 451, 2655
Eco47I	GGWCC	1 cut(s)	1658
Eco47III	AGCGCT	1 cut(s)	1959
Eco52I	CGGCCG	1 cut(s)	2525
Eco57I	CTGAAG	1 cut(s)	33
Eco88I	CYCGRG	1 cut(s)	676
EcoO109I	RGGNCCY	1 cut(s)	1642
EcoRII	CCWGG	6 cut(s)	460, 1107, 1363, 1883, 2045, 2664
EcoT14I	CCWWGG	3 cut(s)	20, 170, 1462
ErhI	CCWWGG	3 cut(s)	20, 170, 1462
Esp3I	CGTCTC	1 cut(s)	221
FalI	AAGNNNNNCTT	4 cut(s)	2160, 2192, 2301, 2333
FaqI	GGGAC	1 cut(s)	659
FauI	CCCGC	1 cut(s)	2694
FauNDI	CATATG	2 cut(s)	1137, 2715
FbaI	TGATCA	2 cut(s)	996, 2544
FokI	GGATG	5 cut(s)	17, 458, 547, 588, 2858
FspBI	CTAG	7 cut(s)	210, 1170, 1430, 1439, 1844, 2487, 2802
FspI	TGCGCA	1 cut(s)	328
GlaI	GCGC	4 cut(s)	58, 328, 1468, 1959
GsaI	CCCAGC	2 cut(s)	316, 629
GsuI	CTGGAG	3 cut(s)	309, 475, 2615
HaeII	RGCGCY	2 cut(s)	1470, 1961
HaeIII	GGCC	7 cut(s)	466, 1494, 1644, 1809, 1836, 2527, 2686
HapII	CCGG	2 cut(s)	1765, 2925
HgaI	GACGC	3 cut(s)	14, 2755, 2915
HhaI	GCGC	4 cut(s)	59, 329, 1469, 1960
Hin1I	GRCGYC	2 cut(s)	25, 2907
Hin6I	GCGC	4 cut(s)	57, 327, 1467, 1958
HinP1I	GCGC	4 cut(s)	57, 327, 1467, 1958
HincII	GTYRAC	2 cut(s)	1459, 2038
HindII	GTYRAC	2 cut(s)	1459, 2038
HindIII	AAGCTT	2 cut(s)	1557, 2722
HpaI	GTTAAC	1 cut(s)	1459
HpaII	CCGG	2 cut(s)	1765, 2925
HphI	GGTGA	4 cut(s)	917, 1579, 2227, 2803
Hpy99I	CGWCG	1 cut(s)	30
HpyAV	CCTTC	7 cut(s)	306, 498, 1034, 1928, 1937, 2162, 2953
HpyCH4III	ACNGT	9 cut(s)	617, 807, 1018, 1453, 1816, 1825, 2711, 2838, 2857
HpyCH4IV	ACGT	3 cut(s)	128, 166, 282
HpyF10VI	GCNNNNNNNGC	7 cut(s)	260, 662, 1550, 1913, 1922, 1925, 1955
HpySE526I	ACGT	3 cut(s)	128, 166, 282
Hsp92I	GRCGYC	2 cut(s)	25, 2907
HspAI	GCGC	4 cut(s)	57, 327, 1467, 1958
Ksp22I	TGATCA	2 cut(s)	996, 2544
KspAI	GTTAAC	1 cut(s)	1459
LmnI	GCTCC	4 cut(s)	316, 707, 1297, 1946
LweI	GCATC	3 cut(s)	586, 1553, 2881
MaeI	CTAG	7 cut(s)	210, 1170, 1430, 1439, 1844, 2487, 2802
MaeII	ACGT	3 cut(s)	128, 166, 282
MaeIII	GTNAC	7 cut(s)	7, 283, 619, 2062, 2460, 2533, 2567
MflI	RGATCY	3 cut(s)	292, 434, 1481
MhlI	GDGCHC	2 cut(s)	193, 1134
MlsI	TGGCCA	3 cut(s)	1494, 1836, 2686
MluNI	TGGCCA	3 cut(s)	1494, 1836, 2686
MlyI	GAGTC	6 cut(s)	934, 1705, 1740, 2033, 2412, 2912
MmeI	TCCRAC	3 cut(s)	1047, 1459, 1685
Mox20I	TGGCCA	3 cut(s)	1494, 1836, 2686
MroXI	GAANNNNTTC	2 cut(s)	1374, 2172
MscI	TGGCCA	3 cut(s)	1494, 1836, 2686
MslI	CAYNNNNRTG	5 cut(s)	690, 1140, 1383, 1401, 2393
Msp20I	TGGCCA	3 cut(s)	1494, 1836, 2686
MspA1I	CMGCKG	2 cut(s)	931, 1253
MspCI	CTTAAG	1 cut(s)	763
MspI	CCGG	2 cut(s)	1765, 2925
MspR9I	CCNGG	7 cut(s)	462, 1109, 1365, 1765, 1885, 2047, 2666
Mva1269I	GAATGC	1 cut(s)	650
MvaI	CCWGG	6 cut(s)	462, 1109, 1365, 1885, 2047, 2666
MvnI	CGCG	2 cut(s)	57, 2749
MwoI	GCNNNNNNNGC	7 cut(s)	260, 662, 1550, 1913, 1922, 1925, 1955
NciI	CCSGG	1 cut(s)	1765
NcoI	CCATGG	2 cut(s)	20, 1462
NdeI	CATATG	2 cut(s)	1137, 2715
NlaIV	GGNNCC	8 cut(s)	318, 804, 1071, 1206, 1612, 1948, 2088, 2821
NmeAIII	GCCGAG	1 cut(s)	2553
NmuCI	GTSAC	3 cut(s)	2062, 2460, 2567
NsbI	TGCGCA	1 cut(s)	328
NspI	RCATGY	1 cut(s)	1313
NspV	TTCGAA	1 cut(s)	1538
PagI	TCATGA	1 cut(s)	1378
PasI	CCCWGGG	1 cut(s)	1364
PcsI	WCGNNNNNNNCGW	1 cut(s)	1720
PctI	GAATGC	1 cut(s)	650
PdmI	GAANNNNTTC	2 cut(s)	1374, 2172
PflMI	CCANNNNNTGG	2 cut(s)	956, 2682
PleI	GAGTC	6 cut(s)	934, 1705, 1739, 2033, 2412, 2912
PpsI	GAGTC	6 cut(s)	934, 1705, 1739, 2033, 2412, 2912
PsiI	TTATAA	1 cut(s)	521
Psp6I	CCWGG	6 cut(s)	460, 1107, 1363, 1883, 2045, 2664
PspFI	CCCAGC	2 cut(s)	312, 625
PspGI	CCWGG	6 cut(s)	460, 1107, 1363, 1883, 2045, 2664
PspN4I	GGNNCC	8 cut(s)	318, 804, 1071, 1206, 1612, 1948, 2088, 2821
PspPI	GGNCC	3 cut(s)	464, 1642, 1658
PstI	CTGCAG	5 cut(s)	1525, 1857, 1918, 1930, 2079
PsuI	RGATCY	3 cut(s)	292, 434, 1481
PvuII	CAGCTG	2 cut(s)	931, 1253
RsaI	GTAC	3 cut(s)	1015, 1651, 1704
RsaNI	GTAC	3 cut(s)	1014, 1650, 1703
RseI	CAYNNNNRTG	5 cut(s)	690, 1140, 1383, 1401, 2393
Sau96I	GGNCC	3 cut(s)	464, 1642, 1658
SbfI	CCTGCAGG	1 cut(s)	2079
SchI	GAGTC	6 cut(s)	934, 1705, 1740, 2033, 2412, 2912
ScrFI	CCNGG	7 cut(s)	462, 1109, 1365, 1765, 1885, 2047, 2666
SdaI	CCTGCAGG	1 cut(s)	2079
SduI	GDGCHC	2 cut(s)	193, 1134
SfaNI	GCATC	3 cut(s)	586, 1553, 2881
SfcI	CTRYAG	8 cut(s)	666, 1452, 1521, 1824, 1853, 1914, 1926, 2075
SfuI	TTCGAA	1 cut(s)	1538
SinI	GGWCC	1 cut(s)	1658
SmiMI	CAYNNNNRTG	5 cut(s)	690, 1140, 1383, 1401, 2393
SmlI	CTYRAG	3 cut(s)	763, 1797, 2894
SmoI	CTYRAG	3 cut(s)	763, 1797, 2894
SpeI	ACTAGT	1 cut(s)	1438
Sse8387I	CCTGCAGG	1 cut(s)	2079
SsiI	CCGC	5 cut(s)	86, 654, 2367, 2701, 2749
SspMI	CTAG	7 cut(s)	210, 1170, 1430, 1439, 1844, 2487, 2802
StyD4I	CCNGG	7 cut(s)	460, 1107, 1363, 1763, 1883, 2045, 2664
StyI	CCWWGG	3 cut(s)	20, 170, 1462
TaaI	ACNGT	9 cut(s)	617, 807, 1018, 1453, 1816, 1825, 2711, 2838, 2857
TaiI	ACGT	3 cut(s)	131, 169, 285
TaqI	TCGA	9 cut(s)	798, 1056, 1238, 1305, 1538, 1736, 2104, 2166, 2391
TauI	GCSGC	1 cut(s)	2752
TscAI	CASTG	6 cut(s)	1097, 2469, 2554, 2598, 2895, 2977
TseFI	GTSAC	3 cut(s)	2062, 2460, 2567
Tsp45I	GTSAC	3 cut(s)	2062, 2460, 2567
TspGWI	ACGGA	5 cut(s)	34, 362, 588, 2271, 2508
TspRI	CASTG	6 cut(s)	1097, 2469, 2554, 2598, 2895, 2977
Van91I	CCANNNNNTGG	2 cut(s)	956, 2682
Vha464I	CTTAAG	1 cut(s)	763
VneI	GTGCAC	1 cut(s)	189
VpaK11BI	GGWCC	1 cut(s)	1658
XapI	RAATTY	2 cut(s)	397, 1743
XbaI	TCTAGA	2 cut(s)	1843, 2486
XceI	RCATGY	1 cut(s)	1313
XmnI	GAANNNNTTC	2 cut(s)	1374, 2172
XspI	CTAG	7 cut(s)	210, 1170, 1430, 1439, 1844, 2487, 2802

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.