Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005640

Physical Location & Seq

Genomic Coordinates Reverse (-)

10032999 .. 10040336

Gene Structure 7338 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1130200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3150 bp

ATGGCTTTTCACTGCCCCTGCTCCACTGAAGCACTGGGGTCGCGGCGGCTCATGGGCGTCCCCGAATATCACGCCCGTCTTCGGCGCCACCACCACCTGCTCACGAAGCATACCCAGTTTCCCTCTTCTTCCTCTTGGCTTGTTCGGAGCTCCGTCAGCCTGAGGTTTTTGTCGTCGTATACATGCCATCACATACCTCTCGGAAATGCGTTCAGATTCACTCTTTGTATGGCAAAGCATGGTTCTTTCCTGAGTTCTCCTATGATGTCCATGGAGAAAGGAAAGTCAAGTGCTACTTCTAAAGGGATGTGCAAGATAGTCTGGACAATAGAGGCTGATCTACAAGCTGGCCAATTTCTATACATAACTGGGGATCCTACTGTTCTTGGCTGCTGGGAGCCGGAGATTGCTATACTAATGTCTCCCATGGAACATACAAACTTATGGAAGGCTGAAGTCAAGATTAATGGTGGTGTCAACTTCAAGTATAACTATTTCATAAAGACGGAAAGGTGGCCTTCATATGACATTACCTGGAGACCTGGACCTGAATTGTCTATATCTGTACCCTTACCTGTCAAACAAAGTGGAAAAATTATAGTGCGGGATTCATGGATGAGGACCGATACCACAATGTCTCCAAATAGTTCATGGGGTTCATGGATAGAGGAGGCATATCTTCCAATACAACCATTGTTCTCAGCTCCAGCTAGAGATGAATATGAGATTATGAAATACCTTAAAAGTGATTTGATAGAATCCAAGCCAGCCTTAAATCTTCCTATGGAGAAGCGCACGGTGTATGCTGATGGCAAACTTAACAATGGCTTAATTTCCAGTAGCGACATATTAGAGTTGAACCCATTCTTAAATGAACCAATGGAGGATAATGTGTACTCTGATTGCGACCGTATAGCAAATAGCAGTCAGAGGGGCTCAACTTCTAATAGTTTTTCAACTGAAAGACATTATCCTGTTGAGGAACCCTGGTTACTCCATTCACCTCTCTTCTGCCTTGTGTCTAAGGATAAGATGGGGTCTGATATGTCCAAAAAGAATGACAGCATAAAAGATGGTGCGGAAAATTTGGATGATCCAGAACATTTGTTGCCTGAAGAAAGGAATAACCTAATTTCGAAGGAACCTGTTTCTACCATTATACTGATTAATTCCTCTATATGTACCATGCAAAGGATAGCCCTACTAGAATATGGGAAATTGGTTGAGTTACTACTGGAACCAGTTAAAAGTACAGTGCAGTGTGATAGCGTGTATATAGGAGTGGTTTCGAAACTTGTACCCCATATGGGTGGTGCATTTGTAAATATTGGGAGTTCTAGACCCTCTCTAATGGACATTAAACAAAACAGAGAACCATTCATATTCCCTCCATTCCGAAGGACAAAGAAAAGTGAAGCTAACAACTATATGTTGGATGAGCATGTGACTGCATACGAAAATGAACGTGTGTCACTTGATTATGAAGCGACTGATGACGTCATTGAAATCAGCTCTCAGAATGATTATGTAAAATCAATGCACAATGATGATGACGATGATGAGCATGATATTGAGGATGAATTTGATGTTTCAGATGTTAAGGAAAACATGAATGGTGGTATACTCGATTATGGTGAAGTAGAAGCTGATTTTCTAGAAGGTGAAACTAGTGCTGTACCTGTTGCCATAAATGGTTCTAGCAATTCTCAAATGTCTCATCTCCAGAATAAGAAGGATACAAATACTGTGACTAATGAGAATAAGTGGGCCCGAGTTCAGAAAGGCACTAAAGTTATTGTACAAGTTGTCAAAGAGGGGCTGGGTTCAAAAGGTCCCACACTGACTGCTTATCCAAAATTAAAAAGCAGATTCTGGGTATTGTTAACTCGTTGTGATAGAATCGGAATCTCCAAAAAAATTGGCGGTGTTGAGCGTACACGATTAAAAGTCATAGCAAAAACCTTGCAACCTCTGGGTTTTGGTCTGACGGTAAGAACTGTTGCTGCTGGTCATTCTTTAGAGGAACTGCAAAAGGATTTGGAAGGCCTTGTTTCAACTTGGAAAAACATTACAGAACATGCAAAATCTGCAGCTCTTGCTGCAGATGAAGGTGTGGAAGGGGCCATTCCTGTCATTCTGCATCGGGCAATGGGTCAGACGCTCTCAGTTGTCCAGGATTATTTTAATGAGACGGTTGAAAAAATGGTGGTTGACTCTCCCAGGACATATCATGAGGTTAGCAATTACCTTCAGGAGATTGCCCCTGATCTCTGTGATCGAGTAGAGTTGTACAACAAAAGAGTTCCTCTTTTTGATGAATTTAACATAGAAGAAGAGATCAACAACATGCTCAGTAAAAGGGTTCCACTGGCTAATGGAGGTTCTTTAGTGATAGAGCAAACTGAGGCATTAGTCTCTATCGATGTGAATGGAGGACATGGGATGTTTGGTCATGGAAATTCACAGGAGAAAGCTATTTTAGAAGTCAACCTTGCAGCTGCTAAACAGATTGCAAGGGAGTTACGACTGAGAGATATTGGTGGCATAATCGTGGTAGATTTCATTGACATGGCTGATGAGTCAAACAAGAGATTGGTGTATGAAGAAGTTAAGAAGGCCGTGGAGAGAGACAGATCGATGGTTAAAGTCTCCGAACTGTCTAGGCATGGACTTATGGAAATAACAAGAAAGCGAGTACGACCTAGTGTGACATTTATGATTAGTGAACCGTGTGCTTGCTGTCATGCCACTGGGAGAGTAGAAGCATTAGAGACCTCCTTCTCCAAAATTGAACAAGAAATTTCTCGATTACTTGCAATGATGGAAGAGAGACCGGACCCGGGGAACCCCAAATCCTGGCCGAAGTTTATTCTGAGAGTTGATCATCACATGTGTGAGTACCTAACCTCTGGGAAAAGGACAAGACTTGCATTCTTGAGCAGTTCCCTCAAAGTCTGGATTCTGCTAAAGGTTGCTAGAGGTTTCACTAGAGGTGCATTTGAGCTGAAACCATTTGTGGATGAAAAGGGACACAAAGATCCTCCGCCATTAACGATTTCAATGTTACAATCTTCGGAAGGCAGAGCTAACAATTCTGGCAGAAAGGTGACCTTGTTTCCGATCAAAAAGTGGAAGACCGGGCGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010239 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0031425 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 RNase_E_G

Protein Analysis

1050

Amino Acids

118.04

Weight (kDa)

6.23

Isoelectric Point (pI)

49.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	105 - 191	6e-12	Starch binding domain
RNase_E_G	PF10150	620 - 899	2.2e-87	Ribonuclease E/G family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270
fragaria_vesca	FvH4_6g40410
malus_domestica	MD09G1130200.v1.1 MD17G1105400.v1.1
prunus_persica	Prupe.3G194900_v2.0.a1 Prupe.3G194900_v2.0.a1
pyrus_communis	pycom09g05330 pycom17g10950 pycom17g10970
rosa_chinensis	RchiOBHm_Chr2g0155641
rosa_laevigata	RLG00000020850
rosa_multiflora	Rmu_ssc0000430.1_g000006
rosa_roxburghii	Rroxscaffold_2G00093600
rosa_rugosa	Rorug02G0448700 Rorug02G0448700
rosa_samantha	Rh2AG514800 Rh2BG525700 Rh2CG499700 Rh2DG534600
rosa_wichuraiana	Rw2G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	105
AatII	GACGTC	1 cut(s)	1500
Acc36I	ACCTGC	1 cut(s)	105
AccB1I	GGYRCC	1 cut(s)	84
AccB7I	CCANNNNNTGG	1 cut(s)	2856
AccI	GTMKAC	2 cut(s)	179, 1621
AccII	CGCG	1 cut(s)	43
AciI	CCGC	6 cut(s)	43, 46, 604, 1079, 1923, 3044
AclWI	GGATC	4 cut(s)	368, 381, 1088, 3032
AcoI	YGGCCR	2 cut(s)	349, 2858
AcsI	RAATTY	5 cut(s)	1084, 1580, 2318, 2458, 2799
AcuI	CTGAAG	4 cut(s)	48, 474, 1134, 2234
AcyI	GRCGYC	3 cut(s)	57, 85, 1497
AfiI	CCNNNNNNNGG	5 cut(s)	81, 1191, 1307, 2840, 2856
AflIII	ACRYGT	2 cut(s)	1465, 2889
AgsI	TTSAA	9 cut(s)	484, 859, 957, 1505, 1827, 2055, 2198, 2792, 3060
AhlI	ACTAGT	1 cut(s)	1667
AjnI	CCWGG	6 cut(s)	533, 541, 986, 2172, 2219, 2855
AleI	CACNNNNGTG	1 cut(s)	2892
AloI	GAACNNNNNNTCC	2 cut(s)	2837, 2869
Alw21I	GWGCWC	1 cut(s)	152
AlwI	GGATC	4 cut(s)	368, 381, 1088, 3032
AlwNI	CAGNNNCTG	1 cut(s)	1872
Ama87I	CYCGRG	2 cut(s)	1770, 2839
AoxI	GGCC	7 cut(s)	349, 515, 1767, 2044, 2121, 2616, 2858
ApaI	GGGCCC	1 cut(s)	1771
ApeKI	GCWGC	6 cut(s)	390, 2003, 2090, 2099, 2495, 2498
ApoI	RAATTY	5 cut(s)	1084, 1580, 2318, 2458, 2799
AseI	ATTAAT	2 cut(s)	465, 1167
Asp700I	GAANNNNTTC	1 cut(s)	863
AspLEI	GCGC	2 cut(s)	87, 795
AspS9I	GGNCC	7 cut(s)	545, 621, 1767, 1768, 1832, 2121, 2836
AsuC2I	CCSGG	3 cut(s)	2840, 2841, 3139
AsuHPI	GGTGA	4 cut(s)	993, 1646, 1673, 3118
AsuII	TTCGAA	2 cut(s)	1136, 1289
AvaI	CYCGRG	2 cut(s)	1770, 2839
AvaII	GGWCC	4 cut(s)	545, 621, 1832, 2836
AxyI	CCTNAGG	1 cut(s)	161
BaeGI	GKGCMC	1 cut(s)	1771
BalI	TGGCCA	1 cut(s)	351
BamHI	GGATCC	1 cut(s)	373
BanI	GGYRCC	1 cut(s)	84
BanII	GRGCYC	3 cut(s)	152, 938, 1771
BbsI	GAAGAC	2 cut(s)	71, 3140
Bbv12I	GWGCWC	1 cut(s)	152
BbvI	GCAGC	6 cut(s)	377, 1990, 2086, 2102, 2485, 2507
BccI	CCATC	6 cut(s)	195, 803, 1027, 1067, 2632, 2815
BceAI	ACGGC	1 cut(s)	2603
BcgI	CGANNNNNNTGC	2 cut(s)	21, 55
BciT130I	CCWGG	6 cut(s)	535, 543, 988, 2174, 2221, 2857
BciVI	GTATCC	1 cut(s)	1729
BclI	TGATCA	1 cut(s)	2881
BcnI	CCSGG	3 cut(s)	2840, 2841, 3139
BcuI	ACTAGT	1 cut(s)	1667
BfmI	CTRYAG	2 cut(s)	2088, 2100
BfoI	RGCGCY	1 cut(s)	88
BfuAI	ACCTGC	1 cut(s)	105
BfuI	GTATCC	1 cut(s)	1729
BisI	GCNGC	8 cut(s)	44, 47, 391, 2004, 2091, 2100, 2496, 2499
BlsI	GCNGC	8 cut(s)	45, 48, 392, 2005, 2092, 2101, 2497, 2500
Bme1390I	CCNGG	9 cut(s)	535, 543, 988, 2174, 2221, 2840, 2841, 2857, 3139
Bme18I	GGWCC	4 cut(s)	545, 621, 1832, 2836
BmeT110I	CYCGRG	2 cut(s)	1770, 2839
BmgT120I	GGNCC	7 cut(s)	545, 621, 1767, 1768, 1832, 2121, 2836
BmrFI	CCNGG	9 cut(s)	535, 543, 988, 2174, 2221, 2840, 2841, 2857, 3139
BmrI	ACTGGG	4 cut(s)	44, 109, 378, 2760
BmsI	GCATC	1 cut(s)	2149
BmuI	ACTGGG	4 cut(s)	44, 109, 378, 2760
BpiI	GAAGAC	2 cut(s)	71, 3140
BpmI	CTGGAG	3 cut(s)	556, 690, 1706
Bpu14I	TTCGAA	2 cut(s)	1136, 1289
BpuEI	CTTGAG	1 cut(s)	2956
BpuMI	CCSGG	3 cut(s)	2840, 2841, 3139
Bsa29I	ATCGAT	2 cut(s)	2421, 2636
BsaHI	GRCGYC	3 cut(s)	57, 85, 1497
BsaI	GGTCTC	3 cut(s)	532, 2765, 2824
BsaJI	CCNNGG	7 cut(s)	270, 426, 986, 2219, 2619, 2839, 2840
BsaWI	WCCGGW	1 cut(s)	2833
BsaXI	ACNNNNNCTCC	2 cut(s)	622, 652
Bsc4I	CCNNNNNNNGG	5 cut(s)	81, 1191, 1307, 2840, 2856
Bse1I	ACTGG	8 cut(s)	39, 115, 373, 837, 1239, 1241, 2373, 2755
Bse21I	CCTNAGG	1 cut(s)	161
Bse3DI	GCAATG	2 cut(s)	2154, 2823
BseBI	CCWGG	6 cut(s)	535, 543, 988, 2174, 2221, 2857
BseCI	ATCGAT	2 cut(s)	2421, 2636
BseDI	CCNNGG	7 cut(s)	270, 426, 986, 2219, 2619, 2839, 2840
BseGI	GGATG	7 cut(s)	312, 621, 1096, 1441, 1582, 2448, 3025
BseLI	CCNNNNNNNGG	5 cut(s)	81, 1191, 1307, 2840, 2856
BseMI	GCAATG	2 cut(s)	2154, 2823
BseMII	CTCAG	9 cut(s)	152, 242, 714, 1529, 2178, 2365, 2394, 2519, 2864
BseNI	ACTGG	8 cut(s)	39, 115, 373, 837, 1239, 1241, 2373, 2755
BseRI	GAGGAG	1 cut(s)	683
BseSI	GKGCMC	1 cut(s)	1771
BseXI	GCAGC	6 cut(s)	377, 1990, 2086, 2102, 2485, 2507
BseYI	CCCAGC	2 cut(s)	393, 1819
BsgI	GTGCAG	1 cut(s)	1277
Bsh1236I	CGCG	1 cut(s)	43
Bsh1285I	CGRYCG	1 cut(s)	910
BshFI	GGCC	7 cut(s)	351, 517, 1769, 2046, 2123, 2618, 2860
BshNI	GGYRCC	1 cut(s)	84
BshVI	ATCGAT	2 cut(s)	2421, 2636
BsiEI	CGRYCG	1 cut(s)	910
BsiHKAI	GWGCWC	1 cut(s)	152
BsiHKCI	CYCGRG	2 cut(s)	1770, 2839
BsiSI	CCGG	4 cut(s)	401, 2834, 2840, 3138
BslFI	GGGAC	3 cut(s)	44, 1818, 3042
BslI	CCNNNNNNNGG	5 cut(s)	81, 1191, 1307, 2840, 2856
BsmBI	CGTCTC	1 cut(s)	2183
BsmFI	GGGAC	3 cut(s)	44, 1818, 3042
BsmI	GAATGC	1 cut(s)	2930
BsnI	GGCC	7 cut(s)	351, 517, 1769, 2046, 2123, 2618, 2860
Bso31I	GGTCTC	3 cut(s)	532, 2765, 2824
BsoBI	CYCGRG	2 cut(s)	1770, 2839
Bsp119I	TTCGAA	2 cut(s)	1136, 1289
Bsp120I	GGGCCC	1 cut(s)	1767
Bsp1286I	GDGCHC	3 cut(s)	152, 938, 1771
Bsp1407I	TGTACA	2 cut(s)	1798, 2289
Bsp19I	CCATGG	2 cut(s)	270, 426
BspACI	CCGC	6 cut(s)	43, 46, 604, 1079, 1923, 3044
BspANI	GGCC	7 cut(s)	351, 517, 1769, 2046, 2123, 2618, 2860
BspCNI	CTCAG	9 cut(s)	153, 243, 713, 1528, 2177, 2364, 2395, 2520, 2865
BspDI	ATCGAT	2 cut(s)	2421, 2636
BspFNI	CGCG	1 cut(s)	43
BspHI	TCATGA	1 cut(s)	2230
BspMAI	CTGCAG	2 cut(s)	2092, 2104
BspMI	ACCTGC	1 cut(s)	105
BspPI	GGATC	4 cut(s)	368, 381, 1088, 3032
BspT104I	TTCGAA	2 cut(s)	1136, 1289
BspT107I	GGYRCC	1 cut(s)	84
BspTNI	GGTCTC	3 cut(s)	532, 2765, 2824
BsrDI	GCAATG	2 cut(s)	2154, 2823
BsrGI	TGTACA	2 cut(s)	1798, 2289
BsrI	ACTGG	8 cut(s)	39, 115, 373, 837, 1239, 1241, 2373, 2755
BssECI	CCNNGG	7 cut(s)	270, 426, 986, 2219, 2619, 2839, 2840
BssNAI	GTATAC	2 cut(s)	180, 1622
BssNI	GRCGYC	3 cut(s)	57, 85, 1497
BssT1I	CCWWGG	2 cut(s)	270, 426
Bst1107I	GTATAC	2 cut(s)	180, 1622
Bst2UI	CCWGG	6 cut(s)	535, 543, 988, 2174, 2221, 2857
Bst6I	CTCTTC	4 cut(s)	130, 1013, 2328, 2820
BstACI	GRCGYC	3 cut(s)	57, 85, 1497
BstAPI	GCANNNNNTGC	2 cut(s)	2087, 2096
BstAUI	TGTACA	2 cut(s)	1798, 2289
BstBI	TTCGAA	2 cut(s)	1136, 1289
BstC8I	GCNNGC	3 cut(s)	349, 768, 2737
BstDSI	CCRYGG	3 cut(s)	270, 426, 2619
BstEII	GGTNACC	1 cut(s)	3106
BstF5I	GGATG	7 cut(s)	312, 621, 1096, 1441, 1582, 2448, 3025
BstFNI	CGCG	1 cut(s)	43
BstH2I	RGCGCY	1 cut(s)	88
BstHHI	GCGC	2 cut(s)	87, 795
BstMCI	CGRYCG	1 cut(s)	910
BstMWI	GCNNNNNNNGC	5 cut(s)	106, 156, 2087, 2096, 2099
BstNI	CCWGG	6 cut(s)	535, 543, 988, 2174, 2221, 2857
BstNSI	RCATGY	5 cut(s)	186, 1445, 2081, 2350, 2893
BstPI	GGTNACC	1 cut(s)	3106
BstSCI	CCNGG	9 cut(s)	533, 541, 986, 2172, 2219, 2838, 2839, 2855, 3137
BstSFI	CTRYAG	2 cut(s)	2088, 2100
BstSLI	GKGCMC	1 cut(s)	1771
BstUI	CGCG	1 cut(s)	43
BstV1I	GCAGC	6 cut(s)	377, 1990, 2086, 2102, 2485, 2507
BstV2I	GAAGAC	2 cut(s)	71, 3140
BstX2I	RGATCY	2 cut(s)	373, 3037
BstXI	CCANNNNNNTGG	1 cut(s)	1310
BstYI	RGATCY	2 cut(s)	373, 3037
BstZ17I	GTATAC	2 cut(s)	180, 1622
Bsu15I	ATCGAT	2 cut(s)	2421, 2636
Bsu36I	CCTNAGG	1 cut(s)	161
BsuI	GTATCC	1 cut(s)	1729
BsuRI	GGCC	7 cut(s)	351, 517, 1769, 2046, 2123, 2618, 2860
BsuTUI	ATCGAT	2 cut(s)	2421, 2636
BtgI	CCRYGG	3 cut(s)	270, 426, 2619
BtsCI	GGATG	7 cut(s)	312, 621, 1096, 1441, 1582, 2448, 3025
BtsI	GCAGTG	2 cut(s)	10, 1265
BtsIMutI	CAGTG	8 cut(s)	10, 24, 32, 1260, 1265, 1838, 2366, 2748
BveI	ACCTGC	1 cut(s)	105
Cac8I	GCNNGC	3 cut(s)	349, 768, 2737
CaiI	CAGNNNCTG	1 cut(s)	1872
CciI	TCATGA	1 cut(s)	2230
CfoI	GCGC	2 cut(s)	87, 795
Cfr13I	GGNCC	7 cut(s)	545, 621, 1767, 1768, 1832, 2121, 2836
Cfr9I	CCCGGG	1 cut(s)	2839
ClaI	ATCGAT	2 cut(s)	2421, 2636
CseI	GACGC	2 cut(s)	46, 2167
CspCI	CAANNNNNGTGG	2 cut(s)	568, 603
DinI	GGCGCC	1 cut(s)	86
EaeI	YGGCCR	2 cut(s)	349, 2858
Eam1104I	CTCTTC	4 cut(s)	130, 1013, 2328, 2820
EarI	CTCTTC	4 cut(s)	130, 1013, 2328, 2820
EciI	GGCGGA	1 cut(s)	3033
Ecl136II	GAGCTC	1 cut(s)	150
Eco130I	CCWWGG	2 cut(s)	270, 426
Eco147I	AGGCCT	1 cut(s)	2046
Eco24I	GRGCYC	3 cut(s)	152, 938, 1771
Eco31I	GGTCTC	3 cut(s)	532, 2765, 2824
Eco47I	GGWCC	4 cut(s)	545, 621, 1832, 2836
Eco53kI	GAGCTC	1 cut(s)	150
Eco57I	CTGAAG	4 cut(s)	48, 474, 1134, 2234
Eco81I	CCTNAGG	1 cut(s)	161
Eco88I	CYCGRG	2 cut(s)	1770, 2839
Eco91I	GGTNACC	1 cut(s)	3106
EcoICRI	GAGCTC	1 cut(s)	150
EcoO109I	RGGNCCY	1 cut(s)	1832
EcoO65I	GGTNACC	1 cut(s)	3106
EcoRII	CCWGG	6 cut(s)	533, 541, 986, 2172, 2219, 2855
EcoT14I	CCWWGG	2 cut(s)	270, 426
EcoT38I	GRGCYC	3 cut(s)	152, 938, 1771
EgeI	GGCGCC	1 cut(s)	86
EheI	GGCGCC	1 cut(s)	86
ErhI	CCWWGG	2 cut(s)	270, 426
Esp3I	CGTCTC	1 cut(s)	2183
FaqI	GGGAC	3 cut(s)	44, 1818, 3042
FauI	CCCGC	1 cut(s)	597
FauNDI	CATATG	2 cut(s)	523, 1305
FbaI	TGATCA	1 cut(s)	2881
FblI	GTMKAC	2 cut(s)	179, 1621
Fnu4HI	GCNGC	8 cut(s)	44, 47, 391, 2004, 2091, 2100, 2496, 2499
FokI	GGATG	7 cut(s)	319, 628, 1103, 1448, 1589, 2455, 3032
FriOI	GRGCYC	3 cut(s)	152, 938, 1771
Fsp4HI	GCNGC	8 cut(s)	44, 47, 391, 2004, 2091, 2100, 2496, 2499
GlaI	GCGC	2 cut(s)	86, 794
GluI	GCNGC	8 cut(s)	44, 47, 391, 2004, 2091, 2100, 2496, 2499
GsaI	CCCAGC	2 cut(s)	397, 1823
GsuI	CTGGAG	3 cut(s)	556, 690, 1706
HaeII	RGCGCY	1 cut(s)	88
HaeIII	GGCC	7 cut(s)	351, 517, 1769, 2046, 2123, 2618, 2860
HapII	CCGG	4 cut(s)	401, 2834, 2840, 3138
HgaI	GACGC	2 cut(s)	46, 2167
HhaI	GCGC	2 cut(s)	87, 795
Hin1I	GRCGYC	3 cut(s)	57, 85, 1497
Hin6I	GCGC	2 cut(s)	85, 793
HinP1I	GCGC	2 cut(s)	85, 793
HincII	GTYRAC	4 cut(s)	478, 1884, 2212, 2488
HindII	GTYRAC	4 cut(s)	478, 1884, 2212, 2488
HinfI	GANTC	9 cut(s)	216, 608, 758, 1869, 1899, 1905, 2213, 2579, 2959
HpaI	GTTAAC	1 cut(s)	1884
HpaII	CCGG	4 cut(s)	401, 2834, 2840, 3138
HphI	GGTGA	4 cut(s)	993, 1646, 1673, 3118
Hpy166II	GTNNAC	9 cut(s)	180, 478, 895, 1622, 1884, 1937, 2212, 2488, 2726
Hpy8I	GTNNAC	9 cut(s)	180, 478, 895, 1622, 1884, 1937, 2212, 2488, 2726
Hpy99I	CGWCG	1 cut(s)	178
HpyCH4IV	ACGT	2 cut(s)	1465, 1497
HpyF10VI	GCNNNNNNNGC	5 cut(s)	106, 156, 2087, 2096, 2099
HpySE526I	ACGT	2 cut(s)	1465, 1497
Hsp92I	GRCGYC	3 cut(s)	57, 85, 1497
HspAI	GCGC	2 cut(s)	85, 793
KasI	GGCGCC	1 cut(s)	84
Ksp22I	TGATCA	1 cut(s)	2881
KspAI	GTTAAC	1 cut(s)	1884
LmnI	GCTCC	5 cut(s)	26, 147, 155, 397, 709
Lsp1109I	GCAGC	6 cut(s)	377, 1990, 2086, 2102, 2485, 2507
LweI	GCATC	1 cut(s)	2149
MaeII	ACGT	2 cut(s)	1465, 1497
MaeIII	GTNAC	9 cut(s)	990, 1227, 1444, 1470, 1747, 2520, 2707, 3063, 3106
MflI	RGATCY	2 cut(s)	373, 3037
MhlI	GDGCHC	3 cut(s)	152, 938, 1771
MlsI	TGGCCA	1 cut(s)	351
MluNI	TGGCCA	1 cut(s)	351
Mly113I	GGCGCC	1 cut(s)	85
MlyI	GAGTC	2 cut(s)	2207, 2588
MmeI	TCCRAC	1 cut(s)	1413
Mox20I	TGGCCA	1 cut(s)	351
MroXI	GAANNNNTTC	1 cut(s)	863
MscI	TGGCCA	1 cut(s)	351
MslI	CAYNNNNRTG	5 cut(s)	1308, 1545, 2549, 2567, 2892
Msp20I	TGGCCA	1 cut(s)	351
MspA1I	CMGCKG	1 cut(s)	2498
MspI	CCGG	4 cut(s)	401, 2834, 2840, 3138
MspR9I	CCNGG	9 cut(s)	535, 543, 988, 2174, 2221, 2840, 2841, 2857, 3139
Mva1269I	GAATGC	1 cut(s)	2930
MvaI	CCWGG	6 cut(s)	535, 543, 988, 2174, 2221, 2857
MvnI	CGCG	1 cut(s)	43
MwoI	GCNNNNNNNGC	5 cut(s)	106, 156, 2087, 2096, 2099
NarI	GGCGCC	1 cut(s)	85
NciI	CCSGG	3 cut(s)	2840, 2841, 3139
NcoI	CCATGG	2 cut(s)	270, 426
NdeI	CATATG	2 cut(s)	523, 1305
NmuCI	GTSAC	5 cut(s)	1444, 1470, 1747, 2707, 3106
NspI	RCATGY	5 cut(s)	186, 1445, 2081, 2350, 2893
NspV	TTCGAA	2 cut(s)	1136, 1289
OliI	CACNNNNGTG	1 cut(s)	2892
PagI	TCATGA	1 cut(s)	2230
PaqCI	CACCTGC	1 cut(s)	105
PceI	AGGCCT	1 cut(s)	2046
PciI	ACATGT	1 cut(s)	2889
PctI	GAATGC	1 cut(s)	2930
PdmI	GAANNNNTTC	1 cut(s)	863
PfeI	GAWTC	7 cut(s)	216, 608, 758, 1869, 1899, 1905, 2959
PflMI	CCANNNNNTGG	1 cut(s)	2856
PfoI	TCCNGGA	1 cut(s)	2172
PkrI	GCNGC	8 cut(s)	45, 48, 392, 2005, 2092, 2101, 2497, 2500
PleI	GAGTC	2 cut(s)	2207, 2587
PluTI	GGCGCC	1 cut(s)	88
PpsI	GAGTC	2 cut(s)	2207, 2587
PpuMI	RGGWCCY	1 cut(s)	1832
PscI	ACATGT	1 cut(s)	2889
PshBI	ATTAAT	2 cut(s)	465, 1167
Psp124BI	GAGCTC	1 cut(s)	152
Psp5II	RGGWCCY	1 cut(s)	1832
Psp6I	CCWGG	6 cut(s)	533, 541, 986, 2172, 2219, 2855
PspEI	GGTNACC	1 cut(s)	3106
PspFI	CCCAGC	2 cut(s)	393, 1819
PspGI	CCWGG	6 cut(s)	533, 541, 986, 2172, 2219, 2855
PspOMI	GGGCCC	1 cut(s)	1767
PspPI	GGNCC	7 cut(s)	545, 621, 1767, 1768, 1832, 2121, 2836
PspPPI	RGGWCCY	1 cut(s)	1832
PsrI	GAACNNNNNNTAC	2 cut(s)	975, 1007
PstI	CTGCAG	2 cut(s)	2092, 2104
PstNI	CAGNNNCTG	1 cut(s)	1872
PsuI	RGATCY	2 cut(s)	373, 3037
PvuII	CAGCTG	1 cut(s)	2498
RseI	CAYNNNNRTG	5 cut(s)	1308, 1545, 2549, 2567, 2892
SacI	GAGCTC	1 cut(s)	152
SatI	GCNGC	8 cut(s)	44, 47, 391, 2004, 2091, 2100, 2496, 2499
Sau96I	GGNCC	7 cut(s)	545, 621, 1767, 1768, 1832, 2121, 2836
SchI	GAGTC	2 cut(s)	2207, 2588
ScrFI	CCNGG	9 cut(s)	535, 543, 988, 2174, 2221, 2840, 2841, 2857, 3139
SduI	GDGCHC	3 cut(s)	152, 938, 1771
SfaNI	GCATC	1 cut(s)	2149
SfcI	CTRYAG	2 cut(s)	2088, 2100
SfoI	GGCGCC	1 cut(s)	86
SfuI	TTCGAA	2 cut(s)	1136, 1289
SinI	GGWCC	4 cut(s)	545, 621, 1832, 2836
SmaI	CCCGGG	1 cut(s)	2841
SmiMI	CAYNNNNRTG	5 cut(s)	1308, 1545, 2549, 2567, 2892
SmlI	CTYRAG	1 cut(s)	2935
SmoI	CTYRAG	1 cut(s)	2935
SpeI	ACTAGT	1 cut(s)	1667
SseBI	AGGCCT	1 cut(s)	2046
SsiI	CCGC	6 cut(s)	43, 46, 604, 1079, 1923, 3044
SspDI	GGCGCC	1 cut(s)	84
SspI	AATATT	1 cut(s)	1327
SstI	GAGCTC	1 cut(s)	152
StuI	AGGCCT	1 cut(s)	2046
StyD4I	CCNGG	9 cut(s)	533, 541, 986, 2172, 2219, 2838, 2839, 2855, 3137
StyI	CCWWGG	2 cut(s)	270, 426
TaiI	ACGT	2 cut(s)	1468, 1500
TaqI	TCGA	7 cut(s)	1136, 1289, 1626, 2278, 2421, 2636, 2806
TaqII	GACCGA	1 cut(s)	638
TatI	WGTACW	4 cut(s)	894, 1250, 1798, 2289
TauI	GCSGC	2 cut(s)	46, 49
TfiI	GAWTC	7 cut(s)	216, 608, 758, 1869, 1899, 1905, 2959
TscAI	CASTG	8 cut(s)	17, 31, 39, 1260, 1265, 1845, 2373, 2755
TseFI	GTSAC	5 cut(s)	1444, 1470, 1747, 2707, 3106
TseI	GCWGC	6 cut(s)	390, 2003, 2090, 2099, 2495, 2498
Tsp45I	GTSAC	5 cut(s)	1444, 1470, 1747, 2707, 3106
TspGWI	ACGGA	2 cut(s)	142, 521
TspMI	CCCGGG	1 cut(s)	2839
TspRI	CASTG	8 cut(s)	17, 31, 39, 1260, 1265, 1845, 2373, 2755
Van91I	CCANNNNNTGG	1 cut(s)	2856
VpaK11BI	GGWCC	4 cut(s)	545, 621, 1832, 2836
VspI	ATTAAT	2 cut(s)	465, 1167
XapI	RAATTY	5 cut(s)	1084, 1580, 2318, 2458, 2799
XbaI	TCTAGA	2 cut(s)	1337, 1654
XceI	RCATGY	5 cut(s)	186, 1445, 2081, 2350, 2893
XcmI	CCANNNNNNNNNTGG	2 cut(s)	31, 648
XmaI	CCCGGG	1 cut(s)	2839
XmiI	GTMKAC	2 cut(s)	179, 1621
XmnI	GAANNNNTTC	1 cut(s)	863
ZraI	GACGTC	1 cut(s)	1498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.