Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0005640

Physical Location & Seq

Genomic Coordinates Forward (+)

69437307 .. 69445125

Gene Structure 7819 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020850

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3096 bp

ATGGAGGCCGGGCGGCTCATGGACATTCCCGAACCTCATACTTGTCCTCGGCGCCACCGTACCCACTTCTCCTCTTCCTCTTGCCTCTCTCCTTCGCTTAGCGTCAGGTTTCTATCACCGTACATATACCATCACATACCCCTTGGAAATGTGTTCAGATTTTCTCTTTGTACAGCAAAGCAATGCTCTTTGCAAAGAGCTCCTATAAAGTCCATGGAGAAAGGCAAGTCAAATACTTCTCTTAAAGGAATGTGCAATATAGTTTGGACAATCGAGGCTGATCTGGAAGCTGGTCAGTTTTTGTACGTCACTGGGGACCTTTCAGCTCTTGGCTCCTGGGAACCAAAGAACGCTATACTAATGTCTCATACAGAACATACAAACTTATGGAAGGCTGAACTCGAGATTGCTGGTGGTGTAAATTTCAAGTATAACTATTTCACAAAGAGAGAAGTGTGGCCTTCATGTGACATTATTTGGAGACCTGGGCCTGAATTCTCTTTGTCAGTACCCCGAACTGTCAAAGGTGGAAAACTCGTGGTGAGGGACTCATGGATGAGGACTACAATGTCTCCTATTCATCCATGGGGCTCATTGATAGAAGAGACATATCTTCCAGTACAACCTTTATTCTCAGCTCCAGCGTCAGCTACAGATGAGGATGAGATTATGAAATACTTTAGAAGTGATATGTTAGAGTCACTGCCCATCTTAAGTATTCCCATGGAGAAGGGAATGTTAGACTCTGATAGTGAACTTACAGCAAATTCTACACAGAAGGGCTTTATTTCTCATAGTGACATAATAGACCCATTGTTAAGTGATCTTAGGAAGGATAGTGCGGACTCCGATGGTGACCTTGCAGTGAATCCTAGTCAGAGGGTTTCAATTTCTAATAGCTTCTCAATGGAAAGATATCAACCTGTTGAGGAGCCCTGGTTAGTCGAACCACCTCCCTTCTTTCTTGTATCCAAGGATATGAGGGGGTCTGATATGTCAGACAATGGCAACATAGAAGATGGCATCACAAATTTGGATGATACAGGAAACTCTTTGCCTGAAGAAAGCAATAACCTAATTCCCAAGGAACCTGTTTCTACCATTATACTGATTAACTCTTCTATATGTACTATGCAAAGGATAGCTCTACTGGAATATGGGAAACTGGTTGAGCTACTATTGGAACCTGTTAAAAGTACTGTGCAGTGTGATAGCGTTTATCTAGGGGTGGTTACAAAACTTGTCCCCCATATGGGTGGGGCATTTGTAAATATAGGGAATTCTAGACCATCTCTAATGGACATCAAACACAACAGGGAGCCTTTTATATTCCCTCCATTCCGAAGGACAAAGAAAACGGAAGCCAACAGTCATATGTTCGAAGAGCACATGACTGCAGATGAAAATGAACGCATGTCACTTGATTTCGAAGTGACTGATGACATCATGGAAATTAGCTCTCAGGATGATTTTGTAAAATCAATGCACAGTGACGATGAGGAGCATGAAATTGAGGATACATTTGATCTCTCAGATGTTAAGGAACACATGAATGGTAGTATACTTGATTATGGCAAAGTAGAAGCTGATTATCCAGAAGGTGAAACTAGTGCCATTCCTGTTGCCATAAATGGTTCTAGCAATTCTCAAATGTCTCACCCCCAAAATAAGAAGAATGGTGCAAATGCTGTCACTCATGAAAATAAGTGGGCCCGAGTTCAGAAGGGCACTAAAGTTGTTGTACAAGTTGTCAAAGAGGGGTTGGGTTCAAAAGGACCCACACTGACTGCTTATCCAAAATTAAAGAGCAGATTCTGGGTATTGATAACTCGTTGCGATAGAATTGGAATCTCCAAGAAAATCAGTGGTATTGAGCGTACACGATTGAAAGTCATTGCAAAAACTTTGCAGCCTCCAGGTTTTGGTCTGACTGTAAGGACTGTTGCTGCTGGCCATTCTTTAGAGGAACTGCAAAAAGATCTGGAAGGTTTGGTTTTAACTTGGAAGAATATCACGGAGCATGCAAAATCTGCAGCTCTTGCTGCAGATGAAGGTGTGGAAGGAGCTGTTCCTGTTATTCTGCATCGTGCAATGGGTCAGACGCTCTCAGTTGTGCAGGATTATTTTAATGAGACGGTTGAGAAAATGGTAGTTGACTCTCCAAGGACATATCATGAGGTTACCAATTACCTTCAGGAGATTGCCCCTGATCTTTGTGATCGAGTTGAGCTATACAGTAAAAGAATCCCTCTTTTTGATGAATTCAACATTGAAGAAGAGATCAACAATATGCTCAGTAAAAGGGTTCCACTTGCTAATGGAGGTTCTCTAGTGATTGAGCAAACTGAGGCATTAGTATCTGTTGATGTGAATGGGGGTCATGGGATGTTTGGTCAAGGAACCTCACAGGAGAAAGCTATTCTGGAAGTCAACCTTGCAGCTGCCAAACAGATTGCAAGGGAGTTACGACTGAGAGATATTGGGGGCATAATTGTGGTAGATTTCATTGATATGGCTGATGAATCAAATAAGAGATTGGTCTACGAAGAAGCTAAGAAGGCTGTTGAGAGAGATAGATCGATGGTTAAAGTCTCTGAACTGTCGAGGCATGGACTCATGGAAATAACGAGAAAGAGAGTACGACCTAGTGTGACATTTATGATTAGTGAACCATGTACTTGTTGTCATGCCACTGGGAGAGTAGAAGCCTTAGAGACCTCATTCTCCAAAATTGAACAAGAAATTTCTCGATTGCTGGCAATGATGGAACGGAAACCAGACCCAGAGAACCCAAAATCTTGGCCAAAGTTCATTCTGAGAGTTGACCATCACATGTGTGACTACTTAACTTCTGGGAAAAGGACAAGACTTGCATTGTTGAGTAGTTCCCTCAAAGCTTGGATTCTACTAAAGGTGGCTAGAGGTTTCACTAGGGGTGCATTTGAGGTAAAACCATTTACAGATGAAAAGGCACACAAAGATCTGCAGCAAGTCACCATTTCAATGATTCGACCTACAGAAGCCAGAAGAACTAACAATCCTGGAAAAAGGGTGACCCTATTTCCGGTAAAGAAATGGAAGGGTGGTGGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010239 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0031425 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 RNase_E_G

Protein Analysis

1032

Amino Acids

115.56

Weight (kDa)

5.98

Isoelectric Point (pI)

46.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	86 - 176	1.7e-14	Starch binding domain
RNase_E_G	PF10150	601 - 880	1.9e-86	Ribonuclease E/G family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270
fragaria_vesca	FvH4_6g40410
malus_domestica	MD09G1130200.v1.1 MD17G1105400.v1.1
prunus_persica	Prupe.3G194900_v2.0.a1 Prupe.3G194900_v2.0.a1
pyrus_communis	pycom09g05330 pycom17g10950 pycom17g10970
rosa_chinensis	RchiOBHm_Chr2g0155641
rosa_laevigata	RLG00000020850
rosa_multiflora	Rmu_ssc0000430.1_g000006
rosa_roxburghii	Rroxscaffold_2G00093600
rosa_rugosa	Rorug02G0448700 Rorug02G0448700
rosa_samantha	Rh2AG514800 Rh2BG525700 Rh2CG499700 Rh2DG534600
rosa_wichuraiana	Rw2G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	568
AccB1I	GGYRCC	1 cut(s)	51
AccB7I	CCANNNNNTGG	1 cut(s)	1922
AccI	GTMKAC	2 cut(s)	1561, 2541
AciI	CCGC	2 cut(s)	13, 842
AcoI	YGGCCR	2 cut(s)	1951, 2801
AcsI	RAATTY	7 cut(s)	421, 494, 766, 1030, 1279, 2261, 2742
AcuI	CTGAAG	2 cut(s)	1080, 2177
AcyI	GRCGYC	1 cut(s)	52
AfiI	CCNNNNNNNGG	4 cut(s)	1252, 1253, 1922, 3064
AflII	CTTAAG	1 cut(s)	712
AflIII	ACRYGT	1 cut(s)	2832
AgsI	TTSAA	8 cut(s)	427, 888, 1770, 1888, 2266, 2273, 2735, 3003
AhlI	ACTAGT	1 cut(s)	1607
AjnI	CCWGG	5 cut(s)	335, 484, 935, 1915, 3040
AleI	CACNNNNGTG	1 cut(s)	2835
AloI	GAACNNNNNNTCC	2 cut(s)	3022, 3054
Alw21I	GWGCWC	2 cut(s)	202, 1389
Alw26I	GTCTC	8 cut(s)	369, 475, 576, 599, 1659, 2126, 2596, 2708
AlwNI	CAGNNNCTG	1 cut(s)	1815
Ama87I	CYCGRG	2 cut(s)	401, 1713
AoxI	GGCC	6 cut(s)	6, 458, 488, 1710, 1951, 2801
ApaI	GGGCCC	1 cut(s)	1714
ApeKI	GCWGC	7 cut(s)	1909, 1946, 2033, 2042, 2438, 2441, 2986
ApoI	RAATTY	7 cut(s)	421, 494, 766, 1030, 1279, 2261, 2742
AspLEI	GCGC	1 cut(s)	54
AspS9I	GGNCC	5 cut(s)	316, 488, 1710, 1711, 1775
AsuC2I	CCSGG	1 cut(s)	10
AsuHPI	GGTGA	7 cut(s)	108, 553, 866, 1613, 1649, 2986, 3064
AsuII	TTCGAA	2 cut(s)	1380, 1428
AvaI	CYCGRG	2 cut(s)	401, 1713
AvaII	GGWCC	2 cut(s)	316, 1775
BaeGI	GKGCMC	2 cut(s)	1714, 1730
BalI	TGGCCA	2 cut(s)	1953, 2803
BanI	GGYRCC	1 cut(s)	51
BanII	GRGCYC	4 cut(s)	202, 593, 936, 1714
BauI	CACGAG	1 cut(s)	536
Bbv12I	GWGCWC	2 cut(s)	202, 1389
BbvI	GCAGC	7 cut(s)	1921, 1933, 2029, 2045, 2428, 2450, 2998
BccI	CCATC	8 cut(s)	138, 716, 845, 1013, 1297, 2575, 2758, 2835
BciT130I	CCWGG	5 cut(s)	337, 486, 937, 1917, 3042
BciVI	GTATCC	2 cut(s)	979, 1510
BcnI	CCSGG	1 cut(s)	10
BcoDI	GTCTC	8 cut(s)	369, 475, 576, 599, 1659, 2126, 2596, 2708
BcuI	ACTAGT	1 cut(s)	1607
BfaI	CTAG	9 cut(s)	873, 1223, 1284, 1608, 1638, 2330, 2646, 2919, 2931
BfmI	CTRYAG	6 cut(s)	651, 1395, 2031, 2043, 2984, 3015
BfoI	RGCGCY	1 cut(s)	55
BfrI	CTTAAG	1 cut(s)	712
BfuI	GTATCC	2 cut(s)	979, 1510
BglII	AGATCT	2 cut(s)	1978, 2980
BisI	GCNGC	8 cut(s)	14, 1910, 1947, 2034, 2043, 2439, 2442, 2987
BlpI	GCTNAGC	1 cut(s)	98
BlsI	GCNGC	8 cut(s)	15, 1911, 1948, 2035, 2044, 2440, 2443, 2988
BmcAI	AGTACT	1 cut(s)	1198
Bme1390I	CCNGG	6 cut(s)	10, 337, 486, 937, 1917, 3042
Bme18I	GGWCC	2 cut(s)	316, 1775
BmeT110I	CYCGRG	2 cut(s)	401, 1713
BmgT120I	GGNCC	5 cut(s)	316, 488, 1710, 1711, 1775
BmrFI	CCNGG	6 cut(s)	10, 337, 486, 937, 1917, 3042
BmrI	ACTGGG	2 cut(s)	321, 2703
BmsI	GCATC	2 cut(s)	1032, 2092
BmuI	ACTGGG	2 cut(s)	321, 2703
BpmI	CTGGAG	2 cut(s)	624, 1899
Bpu1102I	GCTNAGC	1 cut(s)	98
Bpu14I	TTCGAA	2 cut(s)	1380, 1428
BpuMI	CCSGG	1 cut(s)	10
Bsa29I	ATCGAT	1 cut(s)	2579
BsaHI	GRCGYC	1 cut(s)	52
BsaI	GGTCTC	2 cut(s)	475, 2708
BsaWI	WCCGGW	1 cut(s)	3064
BsaXI	ACNNNNNCTCC	4 cut(s)	556, 586, 923, 953
Bsc4I	CCNNNNNNNGG	4 cut(s)	1252, 1253, 1922, 3064
Bse1I	ACTGG	5 cut(s)	316, 617, 1155, 1170, 2698
Bse3DI	GCAATG	4 cut(s)	188, 1893, 2097, 2766
BseBI	CCWGG	5 cut(s)	337, 486, 937, 1917, 3042
BseCI	ATCGAT	1 cut(s)	2579
BseGI	GGATG	6 cut(s)	561, 580, 667, 1042, 1471, 2391
BseLI	CCNNNNNNNGG	4 cut(s)	1252, 1253, 1922, 3064
BseMI	GCAATG	4 cut(s)	188, 1893, 2097, 2766
BseMII	CTCAG	8 cut(s)	648, 1475, 1545, 2121, 2308, 2337, 2462, 2807
BseNI	ACTGG	5 cut(s)	316, 617, 1155, 1170, 2698
BseRI	GAGGAG	3 cut(s)	61, 944, 1514
BseSI	GKGCMC	2 cut(s)	1714, 1730
BseXI	GCAGC	7 cut(s)	1921, 1933, 2029, 2045, 2428, 2450, 2998
BsgI	GTGCAG	2 cut(s)	1223, 2135
BshFI	GGCC	6 cut(s)	8, 460, 490, 1712, 1953, 2803
BshNI	GGYRCC	1 cut(s)	51
BshVI	ATCGAT	1 cut(s)	2579
BsiHKAI	GWGCWC	2 cut(s)	202, 1389
BsiHKCI	CYCGRG	2 cut(s)	401, 1713
BsiSI	CCGG	2 cut(s)	9, 3065
BslFI	GGGAC	3 cut(s)	329, 560, 1229
BslI	CCNNNNNNNGG	4 cut(s)	1252, 1253, 1922, 3064
BsmAI	GTCTC	8 cut(s)	369, 475, 576, 599, 1659, 2126, 2596, 2708
BsmBI	CGTCTC	1 cut(s)	2126
BsmFI	GGGAC	3 cut(s)	329, 560, 1229
BsnI	GGCC	6 cut(s)	8, 460, 490, 1712, 1953, 2803
Bso31I	GGTCTC	2 cut(s)	475, 2708
BsoBI	CYCGRG	2 cut(s)	401, 1713
Bsp119I	TTCGAA	2 cut(s)	1380, 1428
Bsp120I	GGGCCC	1 cut(s)	1710
Bsp1286I	GDGCHC	6 cut(s)	202, 593, 936, 1389, 1714, 1730
Bsp1407I	TGTACA	2 cut(s)	170, 1741
Bsp143I	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
Bsp1720I	GCTNAGC	1 cut(s)	98
Bsp19I	CCATGG	3 cut(s)	213, 584, 723
BspACI	CCGC	2 cut(s)	13, 842
BspANI	GGCC	6 cut(s)	8, 460, 490, 1712, 1953, 2803
BspCNI	CTCAG	8 cut(s)	647, 1474, 1544, 2120, 2307, 2338, 2463, 2808
BspDI	ATCGAT	1 cut(s)	2579
BspHI	TCATGA	2 cut(s)	1696, 2173
BspMAI	CTGCAG	4 cut(s)	1399, 2035, 2047, 2988
BspQI	GCTCTTC	1 cut(s)	1377
BspT104I	TTCGAA	2 cut(s)	1380, 1428
BspT107I	GGYRCC	1 cut(s)	51
BspTI	CTTAAG	1 cut(s)	712
BspTNI	GGTCTC	2 cut(s)	475, 2708
BsrDI	GCAATG	4 cut(s)	188, 1893, 2097, 2766
BsrGI	TGTACA	2 cut(s)	170, 1741
BsrI	ACTGG	5 cut(s)	316, 617, 1155, 1170, 2698
BssMI	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
BssNAI	GTATAC	1 cut(s)	1562
BssNI	GRCGYC	1 cut(s)	52
BssSI	CACGAG	1 cut(s)	536
BssT1I	CCWWGG	7 cut(s)	142, 213, 584, 723, 972, 1083, 2162
Bst1107I	GTATAC	1 cut(s)	1562
Bst2BI	CACGAG	1 cut(s)	536
Bst2UI	CCWGG	5 cut(s)	337, 486, 937, 1917, 3042
Bst6I	CTCTTC	5 cut(s)	79, 597, 1123, 1377, 2271
BstACI	GRCGYC	1 cut(s)	52
BstAFI	CTTAAG	1 cut(s)	712
BstAPI	GCANNNNNTGC	2 cut(s)	2030, 2039
BstAUI	TGTACA	2 cut(s)	170, 1741
BstBI	TTCGAA	2 cut(s)	1380, 1428
BstC8I	GCNNGC	3 cut(s)	1951, 2022, 2757
BstDSI	CCRYGG	3 cut(s)	213, 584, 723
BstEII	GGTNACC	3 cut(s)	854, 2179, 3052
BstF5I	GGATG	6 cut(s)	561, 580, 667, 1042, 1471, 2391
BstH2I	RGCGCY	1 cut(s)	55
BstHHI	GCGC	1 cut(s)	54
BstKTI	GATC	9 cut(s)	283, 826, 1528, 1981, 2212, 2221, 2283, 2579, 2983
BstMAI	GTCTC	8 cut(s)	369, 475, 576, 599, 1659, 2126, 2596, 2708
BstMBI	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
BstMWI	GCNNNNNNNGC	4 cut(s)	2030, 2039, 2042, 2558
BstNI	CCWGG	5 cut(s)	337, 486, 937, 1917, 3042
BstNSI	RCATGY	3 cut(s)	1417, 2024, 2836
BstPI	GGTNACC	3 cut(s)	854, 2179, 3052
BstSCI	CCNGG	6 cut(s)	8, 335, 484, 935, 1915, 3040
BstSFI	CTRYAG	6 cut(s)	651, 1395, 2031, 2043, 2984, 3015
BstSLI	GKGCMC	2 cut(s)	1714, 1730
BstV1I	GCAGC	7 cut(s)	1921, 1933, 2029, 2045, 2428, 2450, 2998
BstX2I	RGATCY	2 cut(s)	1978, 2980
BstXI	CCANNNNNNTGG	2 cut(s)	1256, 2799
BstYI	RGATCY	2 cut(s)	1978, 2980
BstZ17I	GTATAC	1 cut(s)	1562
Bsu15I	ATCGAT	1 cut(s)	2579
BsuI	GTATCC	2 cut(s)	979, 1510
BsuRI	GGCC	6 cut(s)	8, 460, 490, 1712, 1953, 2803
BsuTUI	ATCGAT	1 cut(s)	2579
BtgI	CCRYGG	3 cut(s)	213, 584, 723
BtsCI	GGATG	6 cut(s)	561, 580, 667, 1042, 1471, 2391
BtsI	GCAGTG	3 cut(s)	701, 870, 1211
BtsIMutI	CAGTG	8 cut(s)	309, 701, 870, 1211, 1495, 1781, 1870, 2691
Cac8I	GCNNGC	3 cut(s)	1951, 2022, 2757
CaiI	CAGNNNCTG	1 cut(s)	1815
CciI	TCATGA	2 cut(s)	1696, 2173
CfoI	GCGC	1 cut(s)	54
Cfr13I	GGNCC	5 cut(s)	316, 488, 1710, 1711, 1775
ClaI	ATCGAT	1 cut(s)	2579
CseI	GACGC	3 cut(s)	91, 633, 2110
DinI	GGCGCC	1 cut(s)	53
DpnI	GATC	9 cut(s)	282, 825, 1527, 1980, 2211, 2220, 2282, 2578, 2982
DpnII	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
DrdI	GACNNNNNNGTC	1 cut(s)	568
DseDI	GACNNNNNNGTC	1 cut(s)	568
EaeI	YGGCCR	2 cut(s)	1951, 2801
Eam1104I	CTCTTC	5 cut(s)	79, 597, 1123, 1377, 2271
EarI	CTCTTC	5 cut(s)	79, 597, 1123, 1377, 2271
Ecl136II	GAGCTC	1 cut(s)	200
Eco130I	CCWWGG	7 cut(s)	142, 213, 584, 723, 972, 1083, 2162
Eco24I	GRGCYC	4 cut(s)	202, 593, 936, 1714
Eco31I	GGTCTC	2 cut(s)	475, 2708
Eco32I	GATATC	1 cut(s)	917
Eco47I	GGWCC	2 cut(s)	316, 1775
Eco53kI	GAGCTC	1 cut(s)	200
Eco57I	CTGAAG	2 cut(s)	1080, 2177
Eco88I	CYCGRG	2 cut(s)	401, 1713
Eco91I	GGTNACC	3 cut(s)	854, 2179, 3052
EcoICRI	GAGCTC	1 cut(s)	200
EcoO109I	RGGNCCY	2 cut(s)	316, 1775
EcoO65I	GGTNACC	3 cut(s)	854, 2179, 3052
EcoRI	GAATTC	3 cut(s)	494, 1279, 2261
EcoRII	CCWGG	5 cut(s)	335, 484, 935, 1915, 3040
EcoRV	GATATC	1 cut(s)	917
EcoT14I	CCWWGG	7 cut(s)	142, 213, 584, 723, 972, 1083, 2162
EcoT38I	GRGCYC	4 cut(s)	202, 593, 936, 1714
EgeI	GGCGCC	1 cut(s)	53
EheI	GGCGCC	1 cut(s)	53
ErhI	CCWWGG	7 cut(s)	142, 213, 584, 723, 972, 1083, 2162
Esp3I	CGTCTC	1 cut(s)	2126
FalI	AAGNNNNNCTT	2 cut(s)	2418, 2450
FaqI	GGGAC	3 cut(s)	329, 560, 1229
FauNDI	CATATG	2 cut(s)	1251, 1374
FblI	GTMKAC	2 cut(s)	1561, 2541
Fnu4HI	GCNGC	8 cut(s)	14, 1910, 1947, 2034, 2043, 2439, 2442, 2987
FokI	GGATG	6 cut(s)	567, 568, 674, 1049, 1478, 2398
FriOI	GRGCYC	4 cut(s)	202, 593, 936, 1714
Fsp4HI	GCNGC	8 cut(s)	14, 1910, 1947, 2034, 2043, 2439, 2442, 2987
FspBI	CTAG	9 cut(s)	873, 1223, 1284, 1608, 1638, 2330, 2646, 2919, 2931
GlaI	GCGC	1 cut(s)	53
GluI	GCNGC	8 cut(s)	14, 1910, 1947, 2034, 2043, 2439, 2442, 2987
GsuI	CTGGAG	2 cut(s)	624, 1899
HaeII	RGCGCY	1 cut(s)	55
HaeIII	GGCC	6 cut(s)	8, 460, 490, 1712, 1953, 2803
HapII	CCGG	2 cut(s)	9, 3065
HgaI	GACGC	3 cut(s)	91, 633, 2110
HhaI	GCGC	1 cut(s)	54
Hin1I	GRCGYC	1 cut(s)	52
Hin6I	GCGC	1 cut(s)	52
HinP1I	GCGC	1 cut(s)	52
HincII	GTYRAC	3 cut(s)	2155, 2431, 2824
HindII	GTYRAC	3 cut(s)	2155, 2431, 2824
HindIII	AAGCTT	1 cut(s)	2895
HpaII	CCGG	2 cut(s)	9, 3065
HphI	GGTGA	7 cut(s)	108, 553, 866, 1613, 1649, 2986, 3064
Hpy166II	GTNNAC	8 cut(s)	755, 1562, 1880, 2155, 2431, 2542, 2669, 2824
Hpy8I	GTNNAC	8 cut(s)	755, 1562, 1880, 2155, 2431, 2542, 2669, 2824
HpyCH4IV	ACGT	1 cut(s)	306
HpyF10VI	GCNNNNNNNGC	4 cut(s)	2030, 2039, 2042, 2558
HpySE526I	ACGT	1 cut(s)	306
Hsp92I	GRCGYC	1 cut(s)	52
HspAI	GCGC	1 cut(s)	52
KasI	GGCGCC	1 cut(s)	51
Kzo9I	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
LguI	GCTCTTC	1 cut(s)	1377
LmnI	GCTCC	8 cut(s)	205, 338, 643, 931, 1318, 1501, 2017, 2063
Lsp1109I	GCAGC	7 cut(s)	1921, 1933, 2029, 2045, 2428, 2450, 2998
LweI	GCATC	2 cut(s)	1032, 2092
MaeI	CTAG	9 cut(s)	873, 1223, 1284, 1608, 1638, 2330, 2646, 2919, 2931
MaeII	ACGT	1 cut(s)	306
MalI	GATC	9 cut(s)	282, 825, 1527, 1980, 2211, 2220, 2282, 2578, 2982
MboI	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
MflI	RGATCY	2 cut(s)	1978, 2980
MhlI	GDGCHC	6 cut(s)	202, 593, 936, 1389, 1714, 1730
MlsI	TGGCCA	2 cut(s)	1953, 2803
MluNI	TGGCCA	2 cut(s)	1953, 2803
Mly113I	GGCGCC	1 cut(s)	52
MlyI	GAGTC	6 cut(s)	542, 707, 737, 839, 2150, 2607
Mox20I	TGGCCA	2 cut(s)	1953, 2803
MscI	TGGCCA	2 cut(s)	1953, 2803
MslI	CAYNNNNRTG	6 cut(s)	1254, 1551, 2492, 2510, 2835, 3002
Msp20I	TGGCCA	2 cut(s)	1953, 2803
MspA1I	CMGCKG	1 cut(s)	2441
MspCI	CTTAAG	1 cut(s)	712
MspI	CCGG	2 cut(s)	9, 3065
MspR9I	CCNGG	6 cut(s)	10, 337, 486, 937, 1917, 3042
MvaI	CCWGG	5 cut(s)	337, 486, 937, 1917, 3042
MwoI	GCNNNNNNNGC	4 cut(s)	2030, 2039, 2042, 2558
NarI	GGCGCC	1 cut(s)	52
NciI	CCSGG	1 cut(s)	10
NcoI	CCATGG	3 cut(s)	213, 584, 723
NdeI	CATATG	2 cut(s)	1251, 1374
NdeII	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
NmeAIII	GCCGAG	1 cut(s)	28
NspI	RCATGY	3 cut(s)	1417, 2024, 2836
NspV	TTCGAA	2 cut(s)	1380, 1428
OliI	CACNNNNGTG	1 cut(s)	2835
PaeI	GCATGC	1 cut(s)	2024
PaeR7I	CTCGAG	1 cut(s)	401
PagI	TCATGA	2 cut(s)	1696, 2173
PciI	ACATGT	1 cut(s)	2832
PciSI	GCTCTTC	1 cut(s)	1377
PcsI	WCGNNNNNNNCGW	1 cut(s)	55
PfeI	GAWTC	7 cut(s)	868, 1812, 1848, 2244, 2522, 2902, 3007
PflMI	CCANNNNNTGG	1 cut(s)	1922
PfoI	TCCNGGA	1 cut(s)	3040
PkrI	GCNGC	8 cut(s)	15, 1911, 1948, 2035, 2044, 2440, 2443, 2988
PleI	GAGTC	6 cut(s)	542, 706, 737, 839, 2150, 2607
PluTI	GGCGCC	1 cut(s)	55
PpsI	GAGTC	6 cut(s)	542, 706, 737, 839, 2150, 2607
PpuMI	RGGWCCY	2 cut(s)	316, 1775
PscI	ACATGT	1 cut(s)	2832
Psp124BI	GAGCTC	1 cut(s)	202
Psp5II	RGGWCCY	2 cut(s)	316, 1775
Psp6I	CCWGG	5 cut(s)	335, 484, 935, 1915, 3040
PspEI	GGTNACC	3 cut(s)	854, 2179, 3052
PspGI	CCWGG	5 cut(s)	335, 484, 935, 1915, 3040
PspOMI	GGGCCC	1 cut(s)	1710
PspPI	GGNCC	5 cut(s)	316, 488, 1710, 1711, 1775
PspPPI	RGGWCCY	2 cut(s)	316, 1775
PstI	CTGCAG	4 cut(s)	1399, 2035, 2047, 2988
PstNI	CAGNNNCTG	1 cut(s)	1815
PsuI	RGATCY	2 cut(s)	1978, 2980
PvuII	CAGCTG	1 cut(s)	2441
RseI	CAYNNNNRTG	6 cut(s)	1254, 1551, 2492, 2510, 2835, 3002
SacI	GAGCTC	1 cut(s)	202
SapI	GCTCTTC	1 cut(s)	1377
SatI	GCNGC	8 cut(s)	14, 1910, 1947, 2034, 2043, 2439, 2442, 2987
Sau3AI	GATC	9 cut(s)	280, 823, 1525, 1978, 2209, 2218, 2280, 2576, 2980
Sau96I	GGNCC	5 cut(s)	316, 488, 1710, 1711, 1775
ScaI	AGTACT	1 cut(s)	1198
SchI	GAGTC	6 cut(s)	542, 707, 737, 839, 2150, 2607
ScrFI	CCNGG	6 cut(s)	10, 337, 486, 937, 1917, 3042
SduI	GDGCHC	6 cut(s)	202, 593, 936, 1389, 1714, 1730
SfaNI	GCATC	2 cut(s)	1032, 2092
SfcI	CTRYAG	6 cut(s)	651, 1395, 2031, 2043, 2984, 3015
SfoI	GGCGCC	1 cut(s)	53
Sfr274I	CTCGAG	1 cut(s)	401
SfuI	TTCGAA	2 cut(s)	1380, 1428
SinI	GGWCC	2 cut(s)	316, 1775
SlaI	CTCGAG	1 cut(s)	401
SmiMI	CAYNNNNRTG	6 cut(s)	1254, 1551, 2492, 2510, 2835, 3002
SmlI	CTYRAG	2 cut(s)	401, 712
SmoI	CTYRAG	2 cut(s)	401, 712
SpeI	ACTAGT	1 cut(s)	1607
SphI	GCATGC	1 cut(s)	2024
SsiI	CCGC	2 cut(s)	13, 842
SspDI	GGCGCC	1 cut(s)	51
SspMI	CTAG	9 cut(s)	873, 1223, 1284, 1608, 1638, 2330, 2646, 2919, 2931
SstI	GAGCTC	1 cut(s)	202
StyD4I	CCNGG	6 cut(s)	8, 335, 484, 935, 1915, 3040
StyI	CCWWGG	7 cut(s)	142, 213, 584, 723, 972, 1083, 2162
TaiI	ACGT	1 cut(s)	309
TatI	WGTACW	6 cut(s)	170, 619, 1127, 1196, 1741, 2675
TauI	GCSGC	1 cut(s)	16
TfiI	GAWTC	7 cut(s)	868, 1812, 1848, 2244, 2522, 2902, 3007
TscAI	CASTG	8 cut(s)	316, 708, 870, 1211, 1495, 1788, 1870, 2698
TseI	GCWGC	7 cut(s)	1909, 1946, 2033, 2042, 2438, 2441, 2986
TspGWI	ACGGA	3 cut(s)	1373, 2030, 2785
TspRI	CASTG	8 cut(s)	316, 708, 870, 1211, 1495, 1788, 1870, 2698
Van91I	CCANNNNNTGG	1 cut(s)	1922
Vha464I	CTTAAG	1 cut(s)	712
VpaK11BI	GGWCC	2 cut(s)	316, 1775
XapI	RAATTY	7 cut(s)	421, 494, 766, 1030, 1279, 2261, 2742
XbaI	TCTAGA	1 cut(s)	1283
XceI	RCATGY	3 cut(s)	1417, 2024, 2836
XhoI	CTCGAG	1 cut(s)	401
XmiI	GTMKAC	2 cut(s)	1561, 2541
XspI	CTAG	9 cut(s)	873, 1223, 1284, 1608, 1638, 2330, 2646, 2919, 2931
ZrmI	AGTACT	1 cut(s)	1198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.