Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0005640

Physical Location & Seq

Genomic Coordinates Forward (+)

31950067 .. 31957872

Gene Structure 7806 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g40410.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGAGGCCGCGCGGCTCATGGACATTCCCGAACCTCATACTTGTCCTCGGCGCCACCGCACCCACTTCTCCTCTTCGCTCGGCGTTAGGTTTCTACCGCCGTACATATACCATCACATACCCCTTGGAAATGTGTTCAAATTCTCTCTTTGCACAGCGAAGCAATGTTCCATGCAGAGAGCCCCTATAAAGTCCATGGAGAAAGGAAAGTCAAATACTTCTCTTAAAGGAATGTGCAAAATAGTTTGGACAATCGAGGCTGATCTGGAAGCTGGTCAGTTTTTGTACTTAACTGGAGACCTTTCAGCTCTTGGCTCCTGGGAACCAGGGATCGCTATACCAATGTCTCATACAGAACATACAAACTTATGGCAGGCTGAACTCGAGATTGCTGGAGGTGTAAATTTCAAGTATAACTATTTCATAAAGAGAGAAGTGTGGCCTTCTTGTGATATTATTTGGAGACCTGGACCTGAATTGTCTTTGTCAGTACCCCTACCTGTCAAGGGTGGAAAAATTGTGGTGAGGGACTCATGGATGAGGACGACGATGTCTCCTATTTATCCATGGGGCTCATTGATAGAAGAGACATATTTTCCAATACAACCTTTATTCTCAGCTCCAGCTAGAGATGAGGATGAGATTATGAAGTACCTTAGAAGTGATATGTTAGAGTCAGGGCCCATCTTAAGTATTCCCATGGAGAAGGGGAAGTTAGACTCTGATAGTGAACTTACAACAAATTCTACACACAATGGCTTTATTTCTCATAGTGACATAATAGACCCATTGTTAAGTGATATTAGGAAGGATAGTGTGTACTCCGCTGATGACCTTACAGTGAATCCTAGTCAGAGGGTTTCAATTTCTAGTAGCTTGTCAACGGAAAGATATCAACTCGTTGAGGAGCCCTGGTTAGTTGAACCACGTAGCTTCTTTCTTGTGTCTGAGGATATGAATGAGTCTGATTTGTCAGCCAACGGCAACGTAGTAGATGGCATTACAAATTTGGACGATACGGGAAACTCTTTGACTGAAGAAAGCAATAACCTAATTCCCAAGGAACCTGTTTCTACCGTTATACTGATCAATTCATCTATATGTACTATGCAAAGGATAGCTCTACTGGAGCATGGGAAACTGGTTGAGCTACTATTGGAACCTGTTAAGAGTACTGTGCAGTGTGATAGCGTTTATCTAGGGGTGGTTACGAAACTTGTCCCTCATATGGGTGGCGCATTTGTAAACATAGGGAATTCTAGACCCTCTCTAATGGACATCAAACACAACAGAGAGCCTTTCATATTCCCTCCATTCCGAAGGACAAAGAAAACAGAAGCCAACAGTCATATGTTTGAAGAGCACATGACTGCAGATGAAAATGAACACATGTCACTTGATTTCGAAATGACTGATGACATCATAGAAATTAGCTCTCAGGACGATTATGTAAAATCACTGCACAGTGATGATGAGGAGCATGAAATTGAGGATGCATTTGATCTTTCAGATGATAAGGAACACATGAATGGTAGTATACTTGATTATGGCAAAGGAGAAGCTGATTATCCTGAAGGTGAAACTAGTGCTATTCCTGTTGCTATAAATGGTTCTAGTATTTCTCAAATGTCTCACCCACAAAATAAGAAGAATGATGCAAATACTGTCACTCATGAAAATAAGTGGGTGCAAGTTCAGAAGGGCACTAAAGTTGTTGTGCAAGTTGTCAAAGAAGGGTTGGGCTCAAAAGGACCCACACTGACTGCTTATCCAAAATTAAAGAGCAGATTCTGGATATTGATAACTCGTTGTGATAGAATTGGAATCTCCAAGAAAATCAGTGGTATTGAGCGTACACGGTTGAAAGTCATTGCAAAAACTCTGCAGCCTCCAGGTTTTGGTCTGACTGTAAGGACTGTTGCTGCTGGTCATTCTTTAGAGGAACTGCAAAAAGATCTGGAAGGTTTGGTTTCAACTTGGAAGAATATCACGGAGCATGCAAAATCTGCAGCTCTTGCTGCAGATGAAGGAGTGGAAGGAGCCGTCCCTGTTATTCTGCATCGTGCAATGGGTCAGACGCTCTCAGTTGTGCAGGATTATTTTAATGAGACGGTTGAGAAAATGGTAGTCGACTCTCCAAGGACATATCATGAGGTTACCAATTACCTTCAGGAGATTGCCCCAAATCTTTGTGATCGAGTTGAGCTTTTCAGTAAAAGAATCCCTCTTTTTGATGAATTTAACATCGAAGAAGAGATCAACAATATGCTCAGTAAAAGGGTTCCACTTGCTAATGGAGGTTCTCTAGTGATTGAGCAAACTGAGGCATTAGTATCTGTTGATGTGAATGGGGGTCATGGGATGTTTGGTCAAGGAACGTCACAGGAGAAAGCTATTCTGGAAGTCAACCTTGCAGCTGCCAAACAGATTGCAAGGGAGTTACGACTGAGAGATATTGGGGGCATAATTGTGGTAGATTTCATTGATATGGCTGATGAATCAAATAAGAGATTGGTCTACGAAGAAGCTAAGAAGGCTGTTGAGAGAGACAGGTCGATGGTTAAAGTCTCTGAACTGTCTAGGCATGGACTCATGGAAATAACAAGAAAGAGAGTACGACCTAGTGTAACATTTATGATTAGTGAACCATGTTCTTGTTGTCATGCCACTGGGAGAGTGGAAGCCTTAGAGACCTCGTTCTCCAAAATTGAACAAGAAATTTCTCGATTGCTGGCAATGAGGGAACAGAAACCAGACCCAGAGAACCCAAAATCTTGGCCAAAGTTCATTCTGAGAGTTGACCATCACATGTGTGACTACTTAACTTCTGGGAAAAGGACCAGGCTTGCATTGTTGAGTAGTTCCCTCAAAGCTTGGATTTTGTTAAAGGTTGCTAGAGGTTTCACCAGGGGTGCATTTGAGGTGAAACCATTTACAGATGAAAAGGCACACAAAGATCTGCAGCAGGTCACCATTTCGATGATTCGACCTAGAGAAGCCAGAAGAACTAACAATCCTGGAAAAAAGGTGACCCTATTTCCGGTGAAGAAATGGAAGGGTGGTGGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010239 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0031425 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 RNase_E_G

Protein Analysis

1024

Amino Acids

114.73

Weight (kDa)

5.9

Isoelectric Point (pI)

42.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	80 - 165	6.4e-12	Starch binding domain
RNase_E_G	PF10150	593 - 872	1.2e-85	Ribonuclease E/G family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270
fragaria_vesca	FvH4_6g40410
malus_domestica	MD09G1130200.v1.1 MD17G1105400.v1.1
prunus_persica	Prupe.3G194900_v2.0.a1 Prupe.3G194900_v2.0.a1
pyrus_communis	pycom09g05330 pycom17g10950 pycom17g10970
rosa_chinensis	RchiOBHm_Chr2g0155641
rosa_laevigata	RLG00000020850
rosa_multiflora	Rmu_ssc0000430.1_g000006
rosa_roxburghii	Rroxscaffold_2G00093600
rosa_rugosa	Rorug02G0448700 Rorug02G0448700
rosa_samantha	Rh2AG514800 Rh2BG525700 Rh2CG499700 Rh2DG534600
rosa_wichuraiana	Rw2G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	550
Acc36I	ACCTGC	1 cut(s)	2956
AccB1I	GGYRCC	1 cut(s)	51
AccB7I	CCANNNNNTGG	1 cut(s)	1898
AccI	GTMKAC	3 cut(s)	1537, 2130, 2517
AccII	CGCG	2 cut(s)	11, 13
AciI	CCGC	5 cut(s)	9, 13, 58, 98, 825
AclWI	GGATC	1 cut(s)	338
AcoI	YGGCCR	1 cut(s)	2777
AcsI	RAATTY	7 cut(s)	140, 403, 742, 1006, 1255, 2237, 2718
AcuI	CTGAAG	3 cut(s)	1056, 1593, 2153
AcyI	GRCGYC	1 cut(s)	52
AfaI	GTAC	9 cut(s)	104, 287, 492, 653, 821, 1105, 1174, 1855, 2616
AfiI	CCNNNNNNNGG	5 cut(s)	506, 1228, 1229, 1898, 3040
AflII	CTTAAG	1 cut(s)	688
AflIII	ACRYGT	2 cut(s)	1389, 2808
AgsI	TTSAA	8 cut(s)	139, 409, 864, 923, 1358, 1864, 1974, 2711
AhlI	ACTAGT	1 cut(s)	1583
AjnI	CCWGG	8 cut(s)	317, 325, 466, 911, 1891, 2840, 2906, 3016
AleI	CACNNNNGTG	1 cut(s)	2811
AloI	GAACNNNNNNTCC	2 cut(s)	2998, 3030
Alw21I	GWGCWC	1 cut(s)	1365
AlwI	GGATC	1 cut(s)	338
AlwNI	CAGNNNCTG	1 cut(s)	1791
Ama87I	CYCGRG	1 cut(s)	383
AoxI	GGCC	4 cut(s)	6, 440, 680, 2777
ApaI	GGGCCC	1 cut(s)	684
ApeKI	GCWGC	7 cut(s)	1885, 1922, 2009, 2018, 2414, 2417, 2962
ApoI	RAATTY	7 cut(s)	140, 403, 742, 1006, 1255, 2237, 2718
AspLEI	GCGC	3 cut(s)	13, 54, 1238
AspS9I	GGNCC	5 cut(s)	470, 680, 681, 1751, 2838
AsuHPI	GGTGA	8 cut(s)	535, 1589, 1625, 2896, 2935, 2962, 3040, 3055
AsuII	TTCGAA	1 cut(s)	1404
AvaI	CYCGRG	1 cut(s)	383
AvaII	GGWCC	3 cut(s)	470, 1751, 2838
BaeGI	GKGCMC	2 cut(s)	684, 1706
BalI	TGGCCA	1 cut(s)	2779
BanI	GGYRCC	1 cut(s)	51
BanII	GRGCYC	5 cut(s)	184, 575, 684, 912, 1745
Bbv12I	GWGCWC	1 cut(s)	1365
BbvI	GCAGC	7 cut(s)	1897, 1909, 2005, 2021, 2404, 2426, 2974
BccI	CCATC	5 cut(s)	120, 692, 989, 2551, 2811
BceAI	ACGGC	3 cut(s)	85, 997, 2027
BciT130I	CCWGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
BclI	TGATCA	1 cut(s)	1086
BcuI	ACTAGT	1 cut(s)	1583
BfmI	CTRYAG	5 cut(s)	1371, 1883, 2007, 2019, 2960
BfoI	RGCGCY	1 cut(s)	55
BfrI	CTTAAG	1 cut(s)	688
BfuAI	ACCTGC	1 cut(s)	2956
BglII	AGATCT	2 cut(s)	1954, 2956
BisI	GCNGC	9 cut(s)	9, 14, 1886, 1923, 2010, 2019, 2415, 2418, 2963
BlsI	GCNGC	9 cut(s)	10, 15, 1887, 1924, 2011, 2020, 2416, 2419, 2964
BmcAI	AGTACT	1 cut(s)	1174
Bme1390I	CCNGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
Bme18I	GGWCC	3 cut(s)	470, 1751, 2838
BmeT110I	CYCGRG	1 cut(s)	383
BmgT120I	GGNCC	5 cut(s)	470, 680, 681, 1751, 2838
BmrFI	CCNGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
BmrI	ACTGGG	1 cut(s)	2679
BmsI	GCATC	3 cut(s)	1483, 1645, 2068
BmuI	ACTGGG	1 cut(s)	2679
BpmI	CTGGAG	5 cut(s)	315, 414, 606, 1148, 1875
Bpu14I	TTCGAA	1 cut(s)	1404
BsaAI	YACGTR	1 cut(s)	929
BsaHI	GRCGYC	1 cut(s)	52
BsaI	GGTCTC	3 cut(s)	291, 457, 2684
BsaWI	WCCGGW	1 cut(s)	3040
BsaXI	ACNNNNNCTCC	4 cut(s)	538, 568, 899, 929
Bsc4I	CCNNNNNNNGG	5 cut(s)	506, 1228, 1229, 1898, 3040
Bse1I	ACTGG	4 cut(s)	298, 1131, 1146, 2674
Bse3DI	GCAATG	4 cut(s)	170, 1869, 2073, 2742
BseBI	CCWGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
BseGI	GGATG	4 cut(s)	543, 643, 1498, 2367
BseLI	CCNNNNNNNGG	5 cut(s)	506, 1228, 1229, 1898, 3040
BseMI	GCAATG	4 cut(s)	170, 1869, 2073, 2742
BseMII	CTCAG	8 cut(s)	630, 939, 1451, 2097, 2284, 2313, 2438, 2783
BseNI	ACTGG	4 cut(s)	298, 1131, 1146, 2674
BseRI	GAGGAG	3 cut(s)	61, 920, 1490
BseSI	GKGCMC	2 cut(s)	684, 1706
BseXI	GCAGC	7 cut(s)	1897, 1909, 2005, 2021, 2404, 2426, 2974
BsgI	GTGCAG	3 cut(s)	1199, 1445, 2111
Bsh1236I	CGCG	2 cut(s)	11, 13
BshFI	GGCC	4 cut(s)	8, 442, 682, 2779
BshNI	GGYRCC	1 cut(s)	51
BsiHKAI	GWGCWC	1 cut(s)	1365
BsiHKCI	CYCGRG	1 cut(s)	383
BsiSI	CCGG	1 cut(s)	3041
BslFI	GGGAC	3 cut(s)	542, 1205, 2030
BslI	CCNNNNNNNGG	5 cut(s)	506, 1228, 1229, 1898, 3040
BsmBI	CGTCTC	1 cut(s)	2102
BsmFI	GGGAC	3 cut(s)	542, 1205, 2030
BsnI	GGCC	4 cut(s)	8, 442, 682, 2779
Bso31I	GGTCTC	3 cut(s)	291, 457, 2684
BsoBI	CYCGRG	1 cut(s)	383
Bsp119I	TTCGAA	1 cut(s)	1404
Bsp120I	GGGCCC	1 cut(s)	680
Bsp1286I	GDGCHC	7 cut(s)	184, 575, 684, 912, 1365, 1706, 1745
Bsp143I	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
Bsp19I	CCATGG	3 cut(s)	195, 566, 699
BspACI	CCGC	5 cut(s)	9, 13, 58, 98, 825
BspANI	GGCC	4 cut(s)	8, 442, 682, 2779
BspCNI	CTCAG	8 cut(s)	629, 940, 1450, 2096, 2283, 2314, 2439, 2784
BspFNI	CGCG	2 cut(s)	11, 13
BspHI	TCATGA	2 cut(s)	1672, 2149
BspMAI	CTGCAG	5 cut(s)	1375, 1887, 2011, 2023, 2964
BspMI	ACCTGC	1 cut(s)	2956
BspPI	GGATC	1 cut(s)	338
BspQI	GCTCTTC	1 cut(s)	1353
BspT104I	TTCGAA	1 cut(s)	1404
BspT107I	GGYRCC	1 cut(s)	51
BspTI	CTTAAG	1 cut(s)	688
BspTNI	GGTCTC	3 cut(s)	291, 457, 2684
BsrDI	GCAATG	4 cut(s)	170, 1869, 2073, 2742
BsrI	ACTGG	4 cut(s)	298, 1131, 1146, 2674
BssMI	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
BssNAI	GTATAC	1 cut(s)	1538
BssNI	GRCGYC	1 cut(s)	52
BssT1I	CCWWGG	6 cut(s)	124, 195, 566, 699, 1059, 2138
Bst1107I	GTATAC	1 cut(s)	1538
Bst2UI	CCWGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
Bst6I	CTCTTC	4 cut(s)	79, 579, 1353, 2247
BstACI	GRCGYC	1 cut(s)	52
BstAFI	CTTAAG	1 cut(s)	688
BstAPI	GCANNNNNTGC	2 cut(s)	2006, 2015
BstBAI	YACGTR	1 cut(s)	929
BstBI	TTCGAA	1 cut(s)	1404
BstC8I	GCNNGC	4 cut(s)	375, 1998, 2733, 2847
BstDSI	CCRYGG	3 cut(s)	195, 566, 699
BstEII	GGTNACC	3 cut(s)	2155, 2968, 3028
BstF5I	GGATG	4 cut(s)	543, 643, 1498, 2367
BstFNI	CGCG	2 cut(s)	11, 13
BstH2I	RGCGCY	1 cut(s)	55
BstHHI	GCGC	3 cut(s)	13, 54, 1238
BstKTI	GATC	8 cut(s)	265, 333, 1089, 1504, 1957, 2197, 2259, 2959
BstMBI	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
BstMWI	GCNNNNNNNGC	4 cut(s)	2006, 2015, 2018, 2534
BstNI	CCWGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
BstNSI	RCATGY	3 cut(s)	1393, 2000, 2812
BstPI	GGTNACC	3 cut(s)	2155, 2968, 3028
BstSCI	CCNGG	8 cut(s)	317, 325, 466, 911, 1891, 2840, 2906, 3016
BstSFI	CTRYAG	5 cut(s)	1371, 1883, 2007, 2019, 2960
BstSLI	GKGCMC	2 cut(s)	684, 1706
BstUI	CGCG	2 cut(s)	11, 13
BstV1I	GCAGC	7 cut(s)	1897, 1909, 2005, 2021, 2404, 2426, 2974
BstX2I	RGATCY	2 cut(s)	1954, 2956
BstXI	CCANNNNNNTGG	1 cut(s)	2775
BstYI	RGATCY	2 cut(s)	1954, 2956
BstZ17I	GTATAC	1 cut(s)	1538
BsuRI	GGCC	4 cut(s)	8, 442, 682, 2779
BtgI	CCRYGG	3 cut(s)	195, 566, 699
BtsCI	GGATG	4 cut(s)	543, 643, 1498, 2367
BtsI	GCAGTG	2 cut(s)	1187, 1457
BtsIMutI	CAGTG	7 cut(s)	846, 1187, 1457, 1471, 1757, 1846, 2667
BveI	ACCTGC	1 cut(s)	2956
Cac8I	GCNNGC	4 cut(s)	375, 1998, 2733, 2847
CaiI	CAGNNNCTG	1 cut(s)	1791
CciI	TCATGA	2 cut(s)	1672, 2149
CfoI	GCGC	3 cut(s)	13, 54, 1238
Cfr13I	GGNCC	5 cut(s)	470, 680, 681, 1751, 2838
CseI	GACGC	1 cut(s)	2086
Csp6I	GTAC	9 cut(s)	103, 286, 491, 652, 820, 1104, 1173, 1854, 2615
CviQI	GTAC	9 cut(s)	103, 286, 491, 652, 820, 1104, 1173, 1854, 2615
DinI	GGCGCC	1 cut(s)	53
DpnI	GATC	8 cut(s)	264, 332, 1088, 1503, 1956, 2196, 2258, 2958
DpnII	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
DrdI	GACNNNNNNGTC	1 cut(s)	550
DseDI	GACNNNNNNGTC	1 cut(s)	550
EaeI	YGGCCR	1 cut(s)	2777
Eam1104I	CTCTTC	4 cut(s)	79, 579, 1353, 2247
EarI	CTCTTC	4 cut(s)	79, 579, 1353, 2247
Eco130I	CCWWGG	6 cut(s)	124, 195, 566, 699, 1059, 2138
Eco24I	GRGCYC	5 cut(s)	184, 575, 684, 912, 1745
Eco31I	GGTCTC	3 cut(s)	291, 457, 2684
Eco32I	GATATC	1 cut(s)	893
Eco47I	GGWCC	3 cut(s)	470, 1751, 2838
Eco57I	CTGAAG	3 cut(s)	1056, 1593, 2153
Eco88I	CYCGRG	1 cut(s)	383
Eco91I	GGTNACC	3 cut(s)	2155, 2968, 3028
EcoO109I	RGGNCCY	2 cut(s)	680, 1751
EcoO65I	GGTNACC	3 cut(s)	2155, 2968, 3028
EcoRI	GAATTC	1 cut(s)	1255
EcoRII	CCWGG	8 cut(s)	317, 325, 466, 911, 1891, 2840, 2906, 3016
EcoRV	GATATC	1 cut(s)	893
EcoT14I	CCWWGG	6 cut(s)	124, 195, 566, 699, 1059, 2138
EcoT22I	ATGCAT	1 cut(s)	1498
EcoT38I	GRGCYC	5 cut(s)	184, 575, 684, 912, 1745
EgeI	GGCGCC	1 cut(s)	53
EheI	GGCGCC	1 cut(s)	53
ErhI	CCWWGG	6 cut(s)	124, 195, 566, 699, 1059, 2138
Esp3I	CGTCTC	1 cut(s)	2102
FalI	AAGNNNNNCTT	2 cut(s)	2394, 2426
FaqI	GGGAC	3 cut(s)	542, 1205, 2030
FauNDI	CATATG	2 cut(s)	1227, 1350
FbaI	TGATCA	1 cut(s)	1086
FblI	GTMKAC	3 cut(s)	1537, 2130, 2517
Fnu4HI	GCNGC	9 cut(s)	9, 14, 1886, 1923, 2010, 2019, 2415, 2418, 2963
FokI	GGATG	4 cut(s)	550, 650, 1505, 2374
FriOI	GRGCYC	5 cut(s)	184, 575, 684, 912, 1745
Fsp4HI	GCNGC	9 cut(s)	9, 14, 1886, 1923, 2010, 2019, 2415, 2418, 2963
GlaI	GCGC	3 cut(s)	12, 53, 1237
GluI	GCNGC	9 cut(s)	9, 14, 1886, 1923, 2010, 2019, 2415, 2418, 2963
GsuI	CTGGAG	5 cut(s)	315, 414, 606, 1148, 1875
HaeII	RGCGCY	1 cut(s)	55
HaeIII	GGCC	4 cut(s)	8, 442, 682, 2779
HapII	CCGG	1 cut(s)	3041
HgaI	GACGC	1 cut(s)	2086
HhaI	GCGC	3 cut(s)	13, 54, 1238
Hin1I	GRCGYC	1 cut(s)	52
Hin6I	GCGC	3 cut(s)	11, 52, 1236
HinP1I	GCGC	3 cut(s)	11, 52, 1236
HincII	GTYRAC	4 cut(s)	882, 2131, 2407, 2800
HindII	GTYRAC	4 cut(s)	882, 2131, 2407, 2800
HindIII	AAGCTT	1 cut(s)	2871
HpaII	CCGG	1 cut(s)	3041
HphI	GGTGA	8 cut(s)	535, 1589, 1625, 2896, 2935, 2962, 3040, 3055
Hpy99I	CGWCG	1 cut(s)	550
HpyCH4IV	ACGT	3 cut(s)	928, 987, 2378
HpyF10VI	GCNNNNNNNGC	4 cut(s)	2006, 2015, 2018, 2534
HpySE526I	ACGT	3 cut(s)	928, 987, 2378
Hsp92I	GRCGYC	1 cut(s)	52
HspAI	GCGC	3 cut(s)	11, 52, 1236
KasI	GGCGCC	1 cut(s)	51
Ksp22I	TGATCA	1 cut(s)	1086
Kzo9I	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
LguI	GCTCTTC	1 cut(s)	1353
LmnI	GCTCC	7 cut(s)	320, 625, 907, 1129, 1477, 1993, 2039
Lsp1109I	GCAGC	7 cut(s)	1897, 1909, 2005, 2021, 2404, 2426, 2974
LweI	GCATC	3 cut(s)	1483, 1645, 2068
MaeII	ACGT	3 cut(s)	928, 987, 2378
MalI	GATC	8 cut(s)	264, 332, 1088, 1503, 1956, 2196, 2258, 2958
MboI	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
MflI	RGATCY	2 cut(s)	1954, 2956
MhlI	GDGCHC	7 cut(s)	184, 575, 684, 912, 1365, 1706, 1745
MlsI	TGGCCA	1 cut(s)	2779
MluNI	TGGCCA	1 cut(s)	2779
Mly113I	GGCGCC	1 cut(s)	52
MlyI	GAGTC	6 cut(s)	524, 683, 713, 971, 2126, 2583
Mox20I	TGGCCA	1 cut(s)	2779
Mph1103I	ATGCAT	1 cut(s)	1498
MscI	TGGCCA	1 cut(s)	2779
MslI	CAYNNNNRTG	8 cut(s)	1099, 1230, 1467, 1527, 2468, 2486, 2811, 2978
Msp20I	TGGCCA	1 cut(s)	2779
MspA1I	CMGCKG	2 cut(s)	827, 2417
MspCI	CTTAAG	1 cut(s)	688
MspI	CCGG	1 cut(s)	3041
MspR9I	CCNGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
MvaI	CCWGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
MvnI	CGCG	2 cut(s)	11, 13
MwoI	GCNNNNNNNGC	4 cut(s)	2006, 2015, 2018, 2534
NarI	GGCGCC	1 cut(s)	52
NcoI	CCATGG	3 cut(s)	195, 566, 699
NdeI	CATATG	2 cut(s)	1227, 1350
NdeII	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
NmeAIII	GCCGAG	2 cut(s)	28, 60
NmuCI	GTSAC	7 cut(s)	773, 1392, 1666, 2379, 2813, 2968, 3028
NsiI	ATGCAT	1 cut(s)	1498
NspI	RCATGY	3 cut(s)	1393, 2000, 2812
NspV	TTCGAA	1 cut(s)	1404
OliI	CACNNNNGTG	1 cut(s)	2811
PaeI	GCATGC	1 cut(s)	2000
PaeR7I	CTCGAG	1 cut(s)	383
PagI	TCATGA	2 cut(s)	1672, 2149
PciI	ACATGT	2 cut(s)	1389, 2808
PciSI	GCTCTTC	1 cut(s)	1353
PfeI	GAWTC	6 cut(s)	844, 1788, 1824, 2220, 2498, 2983
PflFI	GACNNNGTC	1 cut(s)	550
PflMI	CCANNNNNTGG	1 cut(s)	1898
PfoI	TCCNGGA	1 cut(s)	3016
PkrI	GCNGC	9 cut(s)	10, 15, 1887, 1924, 2011, 2020, 2416, 2419, 2964
PleI	GAGTC	6 cut(s)	524, 682, 713, 970, 2126, 2583
PluTI	GGCGCC	1 cut(s)	55
PpsI	GAGTC	6 cut(s)	524, 682, 713, 970, 2126, 2583
Ppu21I	YACGTR	1 cut(s)	929
PpuMI	RGGWCCY	1 cut(s)	1751
PscI	ACATGT	2 cut(s)	1389, 2808
Psp5II	RGGWCCY	1 cut(s)	1751
Psp6I	CCWGG	8 cut(s)	317, 325, 466, 911, 1891, 2840, 2906, 3016
PspEI	GGTNACC	3 cut(s)	2155, 2968, 3028
PspGI	CCWGG	8 cut(s)	317, 325, 466, 911, 1891, 2840, 2906, 3016
PspOMI	GGGCCC	1 cut(s)	680
PspPI	GGNCC	5 cut(s)	470, 680, 681, 1751, 2838
PspPPI	RGGWCCY	1 cut(s)	1751
PstI	CTGCAG	5 cut(s)	1375, 1887, 2011, 2023, 2964
PstNI	CAGNNNCTG	1 cut(s)	1791
PsuI	RGATCY	2 cut(s)	1954, 2956
PsyI	GACNNNGTC	1 cut(s)	550
PvuII	CAGCTG	1 cut(s)	2417
RsaI	GTAC	9 cut(s)	104, 287, 492, 653, 821, 1105, 1174, 1855, 2616
RsaNI	GTAC	9 cut(s)	103, 286, 491, 652, 820, 1104, 1173, 1854, 2615
RseI	CAYNNNNRTG	8 cut(s)	1099, 1230, 1467, 1527, 2468, 2486, 2811, 2978
SalI	GTCGAC	1 cut(s)	2129
SapI	GCTCTTC	1 cut(s)	1353
SatI	GCNGC	9 cut(s)	9, 14, 1886, 1923, 2010, 2019, 2415, 2418, 2963
Sau3AI	GATC	8 cut(s)	262, 330, 1086, 1501, 1954, 2194, 2256, 2956
Sau96I	GGNCC	5 cut(s)	470, 680, 681, 1751, 2838
ScaI	AGTACT	1 cut(s)	1174
SchI	GAGTC	6 cut(s)	524, 683, 713, 971, 2126, 2583
ScrFI	CCNGG	8 cut(s)	319, 327, 468, 913, 1893, 2842, 2908, 3018
SduI	GDGCHC	7 cut(s)	184, 575, 684, 912, 1365, 1706, 1745
SfaNI	GCATC	3 cut(s)	1483, 1645, 2068
SfcI	CTRYAG	5 cut(s)	1371, 1883, 2007, 2019, 2960
SfoI	GGCGCC	1 cut(s)	53
Sfr274I	CTCGAG	1 cut(s)	383
SfuI	TTCGAA	1 cut(s)	1404
SinI	GGWCC	3 cut(s)	470, 1751, 2838
SlaI	CTCGAG	1 cut(s)	383
SmiMI	CAYNNNNRTG	8 cut(s)	1099, 1230, 1467, 1527, 2468, 2486, 2811, 2978
SmlI	CTYRAG	2 cut(s)	383, 688
SmoI	CTYRAG	2 cut(s)	383, 688
SpeI	ACTAGT	1 cut(s)	1583
SphI	GCATGC	1 cut(s)	2000
SsiI	CCGC	5 cut(s)	9, 13, 58, 98, 825
SspDI	GGCGCC	1 cut(s)	51
StyD4I	CCNGG	8 cut(s)	317, 325, 466, 911, 1891, 2840, 2906, 3016
StyI	CCWWGG	6 cut(s)	124, 195, 566, 699, 1059, 2138
TaiI	ACGT	3 cut(s)	931, 990, 2381
TatI	WGTACW	4 cut(s)	285, 819, 1103, 1172
TauI	GCSGC	2 cut(s)	11, 16
TfiI	GAWTC	6 cut(s)	844, 1788, 1824, 2220, 2498, 2983
TscAI	CASTG	7 cut(s)	846, 1187, 1464, 1471, 1764, 1846, 2674
TseFI	GTSAC	7 cut(s)	773, 1392, 1666, 2379, 2813, 2968, 3028
TseI	GCWGC	7 cut(s)	1885, 1922, 2009, 2018, 2414, 2417, 2962
Tsp45I	GTSAC	7 cut(s)	773, 1392, 1666, 2379, 2813, 2968, 3028
TspGWI	ACGGA	2 cut(s)	899, 2006
TspRI	CASTG	7 cut(s)	846, 1187, 1464, 1471, 1764, 1846, 2674
Tth111I	GACNNNGTC	1 cut(s)	550
Van91I	CCANNNNNTGG	1 cut(s)	1898
Vha464I	CTTAAG	1 cut(s)	688
VpaK11BI	GGWCC	3 cut(s)	470, 1751, 2838
XapI	RAATTY	7 cut(s)	140, 403, 742, 1006, 1255, 2237, 2718
XbaI	TCTAGA	1 cut(s)	1259
XceI	RCATGY	3 cut(s)	1393, 2000, 2812
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2674
XhoI	CTCGAG	1 cut(s)	383
XmiI	GTMKAC	3 cut(s)	1537, 2130, 2517
ZrmI	AGTACT	1 cut(s)	1174
Zsp2I	ATGCAT	1 cut(s)	1498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.