Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005640

Physical Location & Seq

Genomic Coordinates Forward (+)

68612868 .. 68620579

Gene Structure 7712 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G042300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3090 bp

ATGGAGGCCGGGCGGCTCATGGACATTCCCGAACCTCATACTTGTCCTCGGCGCCACCGCACCCTCTTCTCCTCTTCCTCTTGCCTCCCTCCTTCGCTTAGCGTCAGGTTTCTATCGCCGTACATATACCATCACATACCCCTTGGAAATGTGTTCAGATTTTCTCTTTGTACAGCAAAGCAATGCTCTTTGCAAAGAGCTCCTATAAAGTCCATGGAGAAAGGCAAGTCAAATACTTCTCTTAAAGGAATGTGCAATATAGTTTGGACAATCGAGGCTGATCTGGAAGCTGGTCAGTTTTTGTACGTCACTGGGGACCTTTCAGCTCTCGGCTCCTGGGAACCAGAGAACGCTATACTAATGTCTCATGCAGAACATACAAACTTATGGAAGGCTCAACTCGAGATTGCTGGTGGTGTAAATTTCAAGTATAACTATTTCATAAAGAGAGAAGTATGGCCTTCATGTGACATTATTTGGAGACCTGGACCTGAATTCTCTTTGTCAGTACCCCGACCAGTCAACGGTGGAAAACTCGTGGTGAGGGACTCATGGATGAGGACTACGATGTCTCCTATTCATCCATGGGGCTCATTGATAGAAGAGACATATTTTCCAATACAGCCTTTATTCTCAGCTCCAGCTACAGATGAGGATGAGATTATGAAATACCTTAGAAGTGATATGTTAGAGTCACTGCCCGTCTTAAGTATTCCCATGAAGAAGGGAATGTTAGACTCCGATAGTGAACTTACAGCAAATTCTACACAGAAGGGCTTTATTTCTCATAGTGACATAATAGACCCATTGTTAAGTGATCTTAGGAAGGATAGTGCGTACTCTGATGGTGACCTTGCAGTGAATCCTAGTCAGAGGGTTTCAATTTGTAATAGCTTCTCAACGGAAAGATATCAACCTGTTGAGGAGCCCTGGTTAGTCAAACCACCTCCCTTCTTTCTTGTATCCAAGGATATGAGGGGGTCTGATATGTCAGACAATGGCAACATAGAAGATGGCATTACAAATTTGGATGATACAGGAAACTCTTTGCCTGAAGAAAGCAATAACCTAATTCCCAGGGAACCTGTTTCTACCATTATACTGATTAACTCTTCTATATGTACCATGCAAAGGATAGCTCTACTGGAATATGGGAAACTGGTTGAGCTACTATTGGAACCTGTTAAAAGTACTGTGCAGTGTGATAGCGTTTATCTAGGGGTGGTTACAAAACTTGTCCCCCATATGGGTGGGGCATTTGTAAATATAGGGAATTCTAGACCATCTCTAATGGACATCAAACACAACAGGGAGCCTTTTATATTCCCTCCATTCCGAAGGACAAAGAAAATGGAAGCCAACAGTCATATGTTCGAAGAACACATGACTGCAGATGAAAATGAACGCATGTCACTTGATTTCGAAGTGACTGATGACATCATGGAAATTAGCTCTCAGGACGATTTTGTAAAATCAATGCACAGTGACGATGAGGAGCATGAAATTGAGGATGCATTTGATCTCTCAGATGTTAAGGAACACATGAATGGTAGCATACTTGATTATGGCAAAGTAGAAGCTGATTATCCAGAAGGTGAAACTAGTGCCATTCCTGTTGCCATCAATGGTTCTAGCAATTCTCAAATGTCTCACCCCCAAAATAAGAAGAATGGTGCAAATACTGTCACTCATGAAAATAAGTGGGCCCGAGTTCAGAAGGGCACTAAAGTTGTTGTACAAGTTGTCAAAGAGGGGCTGGGTTCAAAAGGACCCACACTGACTGCTTATCCAAAATTAAAGAGCAGATTCTGGATATTGATAACTCGTTGTGATAGAATTGGAATCTCCAAGAAAATCAGTGGTATTGAGCGTACACGATTGAAAGTCATTGCAAAAACTTTGCAGCCTCCAGGTTTTGGTCTGACTGTAAGGACTGTTGCTGCTGGCCATTCTTTAGAGGAACTGCAAAAAGATCTGGAAGGTTTGGTTTTAACTTGGAAGAATATCACGGAGCATGCAAAATCTGCAGCTCTTGCTGCAGATGAAGGTGTGGAAGGAGCCGTTCCTGTTATTCTGCATTGTGCAATGGGTCAGACGCTCTCAGTTGTGCAGGATTATTTTAATGAGACGGTTGAGAAAATGGTAGTTGACTCTCCAAGGACATATCATGAGGTTACCAATTACCTTCAGGAGATTGCCCCTGATCTTTGTGATCGAGTTGAGCTATACAGTAAAAGAATCCCTCTTTTTGATGAATTCAACATTGAAGAAGAGATCAACAATATGCTCAGTAAAAGGGTTCCACTTGCTAATGGAGGTTCTCTAGTGATTGAGCAAACTGAGGCATTAGTATCTGTTGATGTGAATGGGGGTCATGGGATGTTTGGTCAAGGAACCTCACAGGAGAAAGCTATTCTGGAAGTCAACCTTGCAGCTGCCAAACAGATTGCAAGGGAGTTACGACTGAGAGATATTGGGGGCATAATTGTGGTAGATTTCATTGATATGGCCGATGAATCAAATAAGAGATTGGTCTACGAAGAAGCTAAGAAGGCTGTTGAGAGAGATAGATCGATGGTTAAAGTCTCTGAACTGTCTAGGCATGGACTCATGGAAATAACGAGAAAGAGAGTACGACCTAGTGTGACATTTATGATTAGTGAACCATGTACTTGTTGTCATGCCACTGGGAGAGTAGAAGCCTTAGAGACCTCATTCTCCAAAATTGAACAAGAAATTTCTCGATTGCTGGCAATGATGGAACGGAAACCAGACCCAGAGAACCCAAAATCTTGGCCAAAGTTCATTCTGAGAGTTGACCATCACATGTGTGACTACTTAACTTCTGGGAAAAGGACAAGACTTGCATTGTTGAGTAGTTCCCTCAAAGCTTGGATTCTACTAAAGGTGGCTAGAGGTTTCACTAGGGGTGCATTTGAGGTAAAACCATTTACAGATGAAAAGGCACACAAAGATCTGCAGCAAGTCACCATTTCAATGATTCGACCTACAGAAGCCAGAAGAACTAACAATCCTGGAAAAAGGGTGACCCTATTTCCGGTAAAGAAATGGAAGGGTGGTGGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010239 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0031425 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 RNase_E_G

Protein Analysis

1029

Amino Acids

115.4

Weight (kDa)

6.04

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	86 - 173	3.7e-14	Starch binding domain
RNase_E_G	PF10150	599 - 878	2.3e-85	Ribonuclease E/G family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270 AT2G04270
fragaria_vesca	FvH4_6g40410
malus_domestica	MD09G1130200.v1.1 MD17G1105400.v1.1
prunus_persica	Prupe.3G194900_v2.0.a1 Prupe.3G194900_v2.0.a1
pyrus_communis	pycom09g05330 pycom17g10950 pycom17g10970
rosa_chinensis	RchiOBHm_Chr2g0155641
rosa_laevigata	RLG00000020850
rosa_multiflora	Rmu_ssc0000430.1_g000006
rosa_roxburghii	Rroxscaffold_2G00093600
rosa_rugosa	Rorug02G0448700 Rorug02G0448700
rosa_samantha	Rh2AG514800 Rh2BG525700 Rh2CG499700 Rh2DG534600
rosa_wichuraiana	Rw2G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	568
AccB1I	GGYRCC	1 cut(s)	51
AccB7I	CCANNNNNTGG	1 cut(s)	1916
AccI	GTMKAC	1 cut(s)	2535
AciI	CCGC	2 cut(s)	13, 58
AcoI	YGGCCR	3 cut(s)	1945, 2508, 2795
AcsI	RAATTY	7 cut(s)	421, 494, 760, 1024, 1273, 2255, 2736
AcuI	CTGAAG	2 cut(s)	1074, 2171
AcyI	GRCGYC	1 cut(s)	52
AfiI	CCNNNNNNNGG	6 cut(s)	524, 1131, 1246, 1247, 1916, 3058
AflII	CTTAAG	1 cut(s)	706
AflIII	ACRYGT	1 cut(s)	2826
AgsI	TTSAA	8 cut(s)	427, 882, 1764, 1882, 2260, 2267, 2729, 2997
AhlI	ACTAGT	1 cut(s)	1601
AjnI	CCWGG	6 cut(s)	335, 484, 929, 1076, 1909, 3034
AleI	CACNNNNGTG	1 cut(s)	2829
AloI	GAACNNNNNNTCC	2 cut(s)	3016, 3048
Alw21I	GWGCWC	1 cut(s)	202
Alw26I	GTCTC	8 cut(s)	369, 475, 576, 599, 1653, 2120, 2590, 2702
AlwNI	CAGNNNCTG	1 cut(s)	1809
Ama87I	CYCGRG	2 cut(s)	401, 1707
AoxI	GGCC	6 cut(s)	6, 458, 1704, 1945, 2508, 2795
ApaI	GGGCCC	1 cut(s)	1708
ApeKI	GCWGC	7 cut(s)	1903, 1940, 2027, 2036, 2432, 2435, 2980
ApoI	RAATTY	7 cut(s)	421, 494, 760, 1024, 1273, 2255, 2736
AspLEI	GCGC	1 cut(s)	54
AspS9I	GGNCC	5 cut(s)	316, 488, 1704, 1705, 1769
AsuC2I	CCSGG	1 cut(s)	10
AsuHPI	GGTGA	6 cut(s)	553, 860, 1607, 1643, 2980, 3058
AsuII	TTCGAA	2 cut(s)	1374, 1422
AvaI	CYCGRG	2 cut(s)	401, 1707
AvaII	GGWCC	3 cut(s)	316, 488, 1769
BaeGI	GKGCMC	2 cut(s)	1708, 1724
BalI	TGGCCA	2 cut(s)	1947, 2797
BanI	GGYRCC	1 cut(s)	51
BanII	GRGCYC	4 cut(s)	202, 593, 930, 1708
BauI	CACGAG	1 cut(s)	536
Bbv12I	GWGCWC	1 cut(s)	202
BbvI	GCAGC	7 cut(s)	1915, 1927, 2023, 2039, 2422, 2444, 2992
BccI	CCATC	8 cut(s)	138, 839, 1007, 1291, 1628, 2569, 2752, 2829
BceAI	ACGGC	2 cut(s)	103, 2045
BciT130I	CCWGG	6 cut(s)	337, 486, 931, 1078, 1911, 3036
BciVI	GTATCC	1 cut(s)	973
BcnI	CCSGG	1 cut(s)	10
BcoDI	GTCTC	8 cut(s)	369, 475, 576, 599, 1653, 2120, 2590, 2702
BcuI	ACTAGT	1 cut(s)	1601
BfmI	CTRYAG	6 cut(s)	645, 1389, 2025, 2037, 2978, 3009
BfoI	RGCGCY	1 cut(s)	55
BfrI	CTTAAG	1 cut(s)	706
BfuI	GTATCC	1 cut(s)	973
BglII	AGATCT	2 cut(s)	1972, 2974
BisI	GCNGC	8 cut(s)	14, 1904, 1941, 2028, 2037, 2433, 2436, 2981
BlpI	GCTNAGC	1 cut(s)	98
BlsI	GCNGC	8 cut(s)	15, 1905, 1942, 2029, 2038, 2434, 2437, 2982
BmcAI	AGTACT	1 cut(s)	1192
Bme1390I	CCNGG	7 cut(s)	10, 337, 486, 931, 1078, 1911, 3036
Bme18I	GGWCC	3 cut(s)	316, 488, 1769
BmeT110I	CYCGRG	2 cut(s)	401, 1707
BmgT120I	GGNCC	5 cut(s)	316, 488, 1704, 1705, 1769
BmrFI	CCNGG	7 cut(s)	10, 337, 486, 931, 1078, 1911, 3036
BmrI	ACTGGG	2 cut(s)	321, 2697
BmsI	GCATC	1 cut(s)	1501
BmuI	ACTGGG	2 cut(s)	321, 2697
BpmI	CTGGAG	2 cut(s)	624, 1893
Bpu1102I	GCTNAGC	1 cut(s)	98
Bpu14I	TTCGAA	2 cut(s)	1374, 1422
BpuMI	CCSGG	1 cut(s)	10
Bsa29I	ATCGAT	1 cut(s)	2573
BsaHI	GRCGYC	1 cut(s)	52
BsaI	GGTCTC	2 cut(s)	475, 2702
BsaWI	WCCGGW	1 cut(s)	3058
BsaXI	ACNNNNNCTCC	4 cut(s)	556, 586, 917, 947
Bsc4I	CCNNNNNNNGG	6 cut(s)	524, 1131, 1246, 1247, 1916, 3058
Bse1I	ACTGG	5 cut(s)	316, 518, 1149, 1164, 2692
Bse3DI	GCAATG	4 cut(s)	188, 1887, 2091, 2760
BseBI	CCWGG	6 cut(s)	337, 486, 931, 1078, 1911, 3036
BseCI	ATCGAT	1 cut(s)	2573
BseGI	GGATG	6 cut(s)	561, 580, 661, 1036, 1516, 2385
BseLI	CCNNNNNNNGG	6 cut(s)	524, 1131, 1246, 1247, 1916, 3058
BseMI	GCAATG	4 cut(s)	188, 1887, 2091, 2760
BseMII	CTCAG	8 cut(s)	648, 1469, 1539, 2115, 2302, 2331, 2456, 2801
BseNI	ACTGG	5 cut(s)	316, 518, 1149, 1164, 2692
BseRI	GAGGAG	3 cut(s)	61, 938, 1508
BseSI	GKGCMC	2 cut(s)	1708, 1724
BseXI	GCAGC	7 cut(s)	1915, 1927, 2023, 2039, 2422, 2444, 2992
BseYI	CCCAGC	1 cut(s)	1756
BsgI	GTGCAG	2 cut(s)	1217, 2129
BshFI	GGCC	6 cut(s)	8, 460, 1706, 1947, 2510, 2797
BshNI	GGYRCC	1 cut(s)	51
BshVI	ATCGAT	1 cut(s)	2573
BsiHKAI	GWGCWC	1 cut(s)	202
BsiHKCI	CYCGRG	2 cut(s)	401, 1707
BsiSI	CCGG	2 cut(s)	9, 3059
BslFI	GGGAC	3 cut(s)	329, 560, 1223
BslI	CCNNNNNNNGG	6 cut(s)	524, 1131, 1246, 1247, 1916, 3058
BsmAI	GTCTC	8 cut(s)	369, 475, 576, 599, 1653, 2120, 2590, 2702
BsmBI	CGTCTC	1 cut(s)	2120
BsmFI	GGGAC	3 cut(s)	329, 560, 1223
BsnI	GGCC	6 cut(s)	8, 460, 1706, 1947, 2510, 2797
Bso31I	GGTCTC	2 cut(s)	475, 2702
BsoBI	CYCGRG	2 cut(s)	401, 1707
Bsp119I	TTCGAA	2 cut(s)	1374, 1422
Bsp120I	GGGCCC	1 cut(s)	1704
Bsp1286I	GDGCHC	5 cut(s)	202, 593, 930, 1708, 1724
Bsp1407I	TGTACA	2 cut(s)	170, 1735
Bsp143I	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
Bsp1720I	GCTNAGC	1 cut(s)	98
Bsp19I	CCATGG	2 cut(s)	213, 584
BspACI	CCGC	2 cut(s)	13, 58
BspANI	GGCC	6 cut(s)	8, 460, 1706, 1947, 2510, 2797
BspCNI	CTCAG	8 cut(s)	647, 1468, 1538, 2114, 2301, 2332, 2457, 2802
BspDI	ATCGAT	1 cut(s)	2573
BspHI	TCATGA	2 cut(s)	1690, 2167
BspMAI	CTGCAG	4 cut(s)	1393, 2029, 2041, 2982
BspT104I	TTCGAA	2 cut(s)	1374, 1422
BspT107I	GGYRCC	1 cut(s)	51
BspTI	CTTAAG	1 cut(s)	706
BspTNI	GGTCTC	2 cut(s)	475, 2702
BsrDI	GCAATG	4 cut(s)	188, 1887, 2091, 2760
BsrGI	TGTACA	2 cut(s)	170, 1735
BsrI	ACTGG	5 cut(s)	316, 518, 1149, 1164, 2692
BssMI	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
BssNI	GRCGYC	1 cut(s)	52
BssSI	CACGAG	1 cut(s)	536
BssT1I	CCWWGG	5 cut(s)	142, 213, 584, 966, 2156
Bst2BI	CACGAG	1 cut(s)	536
Bst2UI	CCWGG	6 cut(s)	337, 486, 931, 1078, 1911, 3036
Bst6I	CTCTTC	5 cut(s)	71, 79, 597, 1117, 2265
BstACI	GRCGYC	1 cut(s)	52
BstAFI	CTTAAG	1 cut(s)	706
BstAPI	GCANNNNNTGC	2 cut(s)	2024, 2033
BstAUI	TGTACA	2 cut(s)	170, 1735
BstBI	TTCGAA	2 cut(s)	1374, 1422
BstC8I	GCNNGC	3 cut(s)	1945, 2016, 2751
BstDSI	CCRYGG	2 cut(s)	213, 584
BstEII	GGTNACC	3 cut(s)	848, 2173, 3046
BstF5I	GGATG	6 cut(s)	561, 580, 661, 1036, 1516, 2385
BstH2I	RGCGCY	1 cut(s)	55
BstHHI	GCGC	1 cut(s)	54
BstKTI	GATC	9 cut(s)	283, 820, 1522, 1975, 2206, 2215, 2277, 2573, 2977
BstMAI	GTCTC	8 cut(s)	369, 475, 576, 599, 1653, 2120, 2590, 2702
BstMBI	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
BstMWI	GCNNNNNNNGC	4 cut(s)	2024, 2033, 2036, 2552
BstNI	CCWGG	6 cut(s)	337, 486, 931, 1078, 1911, 3036
BstNSI	RCATGY	3 cut(s)	1411, 2018, 2830
BstPI	GGTNACC	3 cut(s)	848, 2173, 3046
BstSCI	CCNGG	7 cut(s)	8, 335, 484, 929, 1076, 1909, 3034
BstSFI	CTRYAG	6 cut(s)	645, 1389, 2025, 2037, 2978, 3009
BstSLI	GKGCMC	2 cut(s)	1708, 1724
BstV1I	GCAGC	7 cut(s)	1915, 1927, 2023, 2039, 2422, 2444, 2992
BstX2I	RGATCY	2 cut(s)	1972, 2974
BstXI	CCANNNNNNTGG	2 cut(s)	1250, 2793
BstYI	RGATCY	2 cut(s)	1972, 2974
Bsu15I	ATCGAT	1 cut(s)	2573
BsuI	GTATCC	1 cut(s)	973
BsuRI	GGCC	6 cut(s)	8, 460, 1706, 1947, 2510, 2797
BsuTUI	ATCGAT	1 cut(s)	2573
BtgI	CCRYGG	2 cut(s)	213, 584
BtsCI	GGATG	6 cut(s)	561, 580, 661, 1036, 1516, 2385
BtsI	GCAGTG	3 cut(s)	695, 864, 1205
BtsIMutI	CAGTG	8 cut(s)	309, 695, 864, 1205, 1489, 1775, 1864, 2685
Cac8I	GCNNGC	3 cut(s)	1945, 2016, 2751
CaiI	CAGNNNCTG	1 cut(s)	1809
CciI	TCATGA	2 cut(s)	1690, 2167
CfoI	GCGC	1 cut(s)	54
Cfr13I	GGNCC	5 cut(s)	316, 488, 1704, 1705, 1769
ClaI	ATCGAT	1 cut(s)	2573
CseI	GACGC	2 cut(s)	91, 2104
DinI	GGCGCC	1 cut(s)	53
DpnI	GATC	9 cut(s)	282, 819, 1521, 1974, 2205, 2214, 2276, 2572, 2976
DpnII	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
DrdI	GACNNNNNNGTC	1 cut(s)	568
DseDI	GACNNNNNNGTC	1 cut(s)	568
EaeI	YGGCCR	3 cut(s)	1945, 2508, 2795
Eam1104I	CTCTTC	5 cut(s)	71, 79, 597, 1117, 2265
EarI	CTCTTC	5 cut(s)	71, 79, 597, 1117, 2265
Ecl136II	GAGCTC	1 cut(s)	200
Eco130I	CCWWGG	5 cut(s)	142, 213, 584, 966, 2156
Eco24I	GRGCYC	4 cut(s)	202, 593, 930, 1708
Eco31I	GGTCTC	2 cut(s)	475, 2702
Eco32I	GATATC	1 cut(s)	911
Eco47I	GGWCC	3 cut(s)	316, 488, 1769
Eco53kI	GAGCTC	1 cut(s)	200
Eco57I	CTGAAG	2 cut(s)	1074, 2171
Eco88I	CYCGRG	2 cut(s)	401, 1707
Eco91I	GGTNACC	3 cut(s)	848, 2173, 3046
EcoICRI	GAGCTC	1 cut(s)	200
EcoO109I	RGGNCCY	2 cut(s)	316, 1769
EcoO65I	GGTNACC	3 cut(s)	848, 2173, 3046
EcoRI	GAATTC	3 cut(s)	494, 1273, 2255
EcoRII	CCWGG	6 cut(s)	335, 484, 929, 1076, 1909, 3034
EcoRV	GATATC	1 cut(s)	911
EcoT14I	CCWWGG	5 cut(s)	142, 213, 584, 966, 2156
EcoT22I	ATGCAT	1 cut(s)	1516
EcoT38I	GRGCYC	4 cut(s)	202, 593, 930, 1708
EgeI	GGCGCC	1 cut(s)	53
EheI	GGCGCC	1 cut(s)	53
ErhI	CCWWGG	5 cut(s)	142, 213, 584, 966, 2156
Esp3I	CGTCTC	1 cut(s)	2120
FalI	AAGNNNNNCTT	2 cut(s)	2412, 2444
FaqI	GGGAC	3 cut(s)	329, 560, 1223
FauNDI	CATATG	2 cut(s)	1245, 1368
FblI	GTMKAC	1 cut(s)	2535
Fnu4HI	GCNGC	8 cut(s)	14, 1904, 1941, 2028, 2037, 2433, 2436, 2981
FokI	GGATG	6 cut(s)	567, 568, 668, 1043, 1523, 2392
FriOI	GRGCYC	4 cut(s)	202, 593, 930, 1708
Fsp4HI	GCNGC	8 cut(s)	14, 1904, 1941, 2028, 2037, 2433, 2436, 2981
GlaI	GCGC	1 cut(s)	53
GluI	GCNGC	8 cut(s)	14, 1904, 1941, 2028, 2037, 2433, 2436, 2981
GsaI	CCCAGC	1 cut(s)	1760
GsuI	CTGGAG	2 cut(s)	624, 1893
HaeII	RGCGCY	1 cut(s)	55
HaeIII	GGCC	6 cut(s)	8, 460, 1706, 1947, 2510, 2797
HapII	CCGG	2 cut(s)	9, 3059
HgaI	GACGC	2 cut(s)	91, 2104
HhaI	GCGC	1 cut(s)	54
Hin1I	GRCGYC	1 cut(s)	52
Hin6I	GCGC	1 cut(s)	52
HinP1I	GCGC	1 cut(s)	52
HincII	GTYRAC	4 cut(s)	523, 2149, 2425, 2818
HindII	GTYRAC	4 cut(s)	523, 2149, 2425, 2818
HindIII	AAGCTT	1 cut(s)	2889
HpaII	CCGG	2 cut(s)	9, 3059
HphI	GGTGA	6 cut(s)	553, 860, 1607, 1643, 2980, 3058
Hpy166II	GTNNAC	8 cut(s)	523, 749, 1874, 2149, 2425, 2536, 2663, 2818
Hpy8I	GTNNAC	8 cut(s)	523, 749, 1874, 2149, 2425, 2536, 2663, 2818
HpyCH4IV	ACGT	1 cut(s)	306
HpyF10VI	GCNNNNNNNGC	4 cut(s)	2024, 2033, 2036, 2552
HpySE526I	ACGT	1 cut(s)	306
Hsp92I	GRCGYC	1 cut(s)	52
HspAI	GCGC	1 cut(s)	52
KasI	GGCGCC	1 cut(s)	51
Kzo9I	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
LmnI	GCTCC	8 cut(s)	205, 338, 643, 925, 1312, 1495, 2011, 2057
Lsp1109I	GCAGC	7 cut(s)	1915, 1927, 2023, 2039, 2422, 2444, 2992
LweI	GCATC	1 cut(s)	1501
MaeII	ACGT	1 cut(s)	306
MalI	GATC	9 cut(s)	282, 819, 1521, 1974, 2205, 2214, 2276, 2572, 2976
MboI	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
MflI	RGATCY	2 cut(s)	1972, 2974
MhlI	GDGCHC	5 cut(s)	202, 593, 930, 1708, 1724
MlsI	TGGCCA	2 cut(s)	1947, 2797
MluNI	TGGCCA	2 cut(s)	1947, 2797
Mly113I	GGCGCC	1 cut(s)	52
MlyI	GAGTC	5 cut(s)	542, 701, 731, 2144, 2601
Mox20I	TGGCCA	2 cut(s)	1947, 2797
Mph1103I	ATGCAT	1 cut(s)	1516
MscI	TGGCCA	2 cut(s)	1947, 2797
MslI	CAYNNNNRTG	6 cut(s)	1248, 1545, 2486, 2504, 2829, 2996
Msp20I	TGGCCA	2 cut(s)	1947, 2797
MspA1I	CMGCKG	1 cut(s)	2435
MspCI	CTTAAG	1 cut(s)	706
MspI	CCGG	2 cut(s)	9, 3059
MspR9I	CCNGG	7 cut(s)	10, 337, 486, 931, 1078, 1911, 3036
MvaI	CCWGG	6 cut(s)	337, 486, 931, 1078, 1911, 3036
MwoI	GCNNNNNNNGC	4 cut(s)	2024, 2033, 2036, 2552
NarI	GGCGCC	1 cut(s)	52
NciI	CCSGG	1 cut(s)	10
NcoI	CCATGG	2 cut(s)	213, 584
NdeI	CATATG	2 cut(s)	1245, 1368
NdeII	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
NmeAIII	GCCGAG	2 cut(s)	28, 309
NsiI	ATGCAT	1 cut(s)	1516
NspI	RCATGY	3 cut(s)	1411, 2018, 2830
NspV	TTCGAA	2 cut(s)	1374, 1422
OliI	CACNNNNGTG	1 cut(s)	2829
PaeI	GCATGC	1 cut(s)	2018
PaeR7I	CTCGAG	1 cut(s)	401
PagI	TCATGA	2 cut(s)	1690, 2167
PasI	CCCWGGG	1 cut(s)	1077
PciI	ACATGT	1 cut(s)	2826
PfeI	GAWTC	7 cut(s)	862, 1806, 1842, 2238, 2516, 2896, 3001
PflMI	CCANNNNNTGG	1 cut(s)	1916
PfoI	TCCNGGA	1 cut(s)	3034
PkrI	GCNGC	8 cut(s)	15, 1905, 1942, 2029, 2038, 2434, 2437, 2982
PleI	GAGTC	5 cut(s)	542, 700, 731, 2144, 2601
PluTI	GGCGCC	1 cut(s)	55
PpsI	GAGTC	5 cut(s)	542, 700, 731, 2144, 2601
PpuMI	RGGWCCY	2 cut(s)	316, 1769
PscI	ACATGT	1 cut(s)	2826
Psp124BI	GAGCTC	1 cut(s)	202
Psp5II	RGGWCCY	2 cut(s)	316, 1769
Psp6I	CCWGG	6 cut(s)	335, 484, 929, 1076, 1909, 3034
PspEI	GGTNACC	3 cut(s)	848, 2173, 3046
PspFI	CCCAGC	1 cut(s)	1756
PspGI	CCWGG	6 cut(s)	335, 484, 929, 1076, 1909, 3034
PspOMI	GGGCCC	1 cut(s)	1704
PspPI	GGNCC	5 cut(s)	316, 488, 1704, 1705, 1769
PspPPI	RGGWCCY	2 cut(s)	316, 1769
PstI	CTGCAG	4 cut(s)	1393, 2029, 2041, 2982
PstNI	CAGNNNCTG	1 cut(s)	1809
PsuI	RGATCY	2 cut(s)	1972, 2974
PvuII	CAGCTG	1 cut(s)	2435
RseI	CAYNNNNRTG	6 cut(s)	1248, 1545, 2486, 2504, 2829, 2996
SacI	GAGCTC	1 cut(s)	202
SatI	GCNGC	8 cut(s)	14, 1904, 1941, 2028, 2037, 2433, 2436, 2981
Sau3AI	GATC	9 cut(s)	280, 817, 1519, 1972, 2203, 2212, 2274, 2570, 2974
Sau96I	GGNCC	5 cut(s)	316, 488, 1704, 1705, 1769
ScaI	AGTACT	1 cut(s)	1192
SchI	GAGTC	5 cut(s)	542, 701, 731, 2144, 2601
ScrFI	CCNGG	7 cut(s)	10, 337, 486, 931, 1078, 1911, 3036
SduI	GDGCHC	5 cut(s)	202, 593, 930, 1708, 1724
SfaNI	GCATC	1 cut(s)	1501
SfcI	CTRYAG	6 cut(s)	645, 1389, 2025, 2037, 2978, 3009
SfoI	GGCGCC	1 cut(s)	53
Sfr274I	CTCGAG	1 cut(s)	401
SfuI	TTCGAA	2 cut(s)	1374, 1422
SinI	GGWCC	3 cut(s)	316, 488, 1769
SlaI	CTCGAG	1 cut(s)	401
SmiMI	CAYNNNNRTG	6 cut(s)	1248, 1545, 2486, 2504, 2829, 2996
SmlI	CTYRAG	2 cut(s)	401, 706
SmoI	CTYRAG	2 cut(s)	401, 706
SpeI	ACTAGT	1 cut(s)	1601
SphI	GCATGC	1 cut(s)	2018
SsiI	CCGC	2 cut(s)	13, 58
SspDI	GGCGCC	1 cut(s)	51
SstI	GAGCTC	1 cut(s)	202
StyD4I	CCNGG	7 cut(s)	8, 335, 484, 929, 1076, 1909, 3034
StyI	CCWWGG	5 cut(s)	142, 213, 584, 966, 2156
TaiI	ACGT	1 cut(s)	309
TaqI	TCGA	8 cut(s)	273, 402, 1374, 1422, 2215, 2573, 2743, 3004
TatI	WGTACW	4 cut(s)	170, 1190, 1735, 2669
TauI	GCSGC	1 cut(s)	16
TfiI	GAWTC	7 cut(s)	862, 1806, 1842, 2238, 2516, 2896, 3001
TscAI	CASTG	8 cut(s)	316, 702, 864, 1205, 1489, 1782, 1864, 2692
TseI	GCWGC	7 cut(s)	1903, 1940, 2027, 2036, 2432, 2435, 2980
TspGWI	ACGGA	3 cut(s)	917, 2024, 2779
TspRI	CASTG	8 cut(s)	316, 702, 864, 1205, 1489, 1782, 1864, 2692
Van91I	CCANNNNNTGG	1 cut(s)	1916
Vha464I	CTTAAG	1 cut(s)	706
VpaK11BI	GGWCC	3 cut(s)	316, 488, 1769
XapI	RAATTY	7 cut(s)	421, 494, 760, 1024, 1273, 2255, 2736
XbaI	TCTAGA	1 cut(s)	1277
XceI	RCATGY	3 cut(s)	1411, 2018, 2830
XhoI	CTCGAG	1 cut(s)	401
XmiI	GTMKAC	1 cut(s)	2535
ZrmI	AGTACT	1 cut(s)	1192
Zsp2I	ATGCAT	1 cut(s)	1516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.