AT2G17130

isocitrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005225
Physical Location & Seq
Reverse (-)
7460814 .. 7462607
1794 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G17130.1

Sequence Viewer

Length: 1104 bp
ATGTCTCGCCAATCGTTTTCTCTACTGAAGAATCTTCGTAGCATCGCTTCCGGTTCCAAAATCCAGACCCGATCCGTGACTTACATGCCCAGACCCGGTGACGGAAAACCAAGACCAGTGACGTTAATCCCCGGAGACGGAGTTGGTCCGTTGGTAACAAACGCGGTTCAACAAGTGATGGAAGCGATGCACGCTCCGGTTTACTTCGAACCGTTCGAAGTTCATGGAGATATGAAGAGTTTACCTGAAGGATTGTTGGAATCGATTAAGAAGAACAAAGTTTGTTTGAAAGGTGGTCTTAAGACTCCCGTCGGTGGTGGTGTGAGCTCTCTCAATGTTAATCTGAGAAAGGAGCTTGATCTCTTTGCTTCTTTGGTCAACTGTTTCAATTTGCCTGGTTTAGCTTCTCGTCATGAGAATGTTGATATTGTTGTGATTAGAGAGAACACTGAAGGTGAATATGCAGGTCTTGAACATGAAGTTGTTCCTGGTGTTGTTGAGAGCCTTAAGGTGATTACAAAGTTCTGTTCAGAGCGTATTGCAAAGTATGCGTTTGAGTATGCCTACTTGAACAACAGGAAGAAAGTTACAGCAGTGCACAAGGCTAATATCATGAAATTGGCTGATGGTTTGTTCTTGGAGTCTTGTCAAGAGGTTGCTAAGAAGTATCCAAGCATTGCTTACAATGAAATCATTGTTGATAATTGTTGTATGCAACTTGTGGCTAGACCAGAGCAATTCGATGTCATGGTGACGCCTAATCTCTATGGTAATCTAGTGGCAAACACTGCTGCTGGTATTGCTGGAGGTACTGGAGTCATGCCTGGAGGAAATGTTGGGGCGGAGTATGCGGTCTTTGAGCAAGGTGCATCAGCGGGAAACGTGGGGAAGGATACAACGGAAGAGCAAAAGAATGCAAACCCTGTGGCTTTGTTGCTGTCATCAGCCATGATGCTTAGACACCTTCAGTTCCCTTCTTTTGCTGACAGGCTTGAAACCGCTGTGAAGCGTGTTATCGCTGAAGGGAATTGTCGGACTGAAGATCTTGGTGGAAACAGTACCACTCAAGAGGTCGTTGACGCTGTCATTGCAAATTTGGATTGA

Protein Analysis

367

Amino Acids

39.59

Weight (kDa)

6.14

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 40 - 362 8.4e-79 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AccII CGCG 1 cut(s) 164
AciI CCGC 5 cut(s) 164, 842, 851, 875, 999
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 1093
AcuI CTGAAG 6 cut(s) 47, 267, 471, 950, 1041, 1059
AcyI GRCGYC 1 cut(s) 755
AfaI GTAC 2 cut(s) 811, 1060
AfiI CCNNNNNNNGG 4 cut(s) 95, 101, 137, 314
AflII CTTAAG 2 cut(s) 299, 506
AgsI TTSAA 6 cut(s) 170, 289, 388, 473, 571, 995
AjnI CCWGG 3 cut(s) 394, 487, 823
AluBI AGCT 3 cut(s) 327, 355, 404
AluI AGCT 3 cut(s) 327, 355, 404
Alw21I GWGCWC 2 cut(s) 329, 600
Alw26I GTCTC 2 cut(s) 9, 129
Alw44I GTGCAC 1 cut(s) 596
AlwI GGATC 1 cut(s) 66
ApaLI GTGCAC 1 cut(s) 596
ApeKI GCWGC 1 cut(s) 791
ApoI RAATTY 1 cut(s) 1093
Asp700I GAANNNNTTC 1 cut(s) 483
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 2 cut(s) 96, 132
AsuHPI GGTGA 4 cut(s) 110, 467, 523, 763
AsuII TTCGAA 2 cut(s) 207, 216
AvaII GGWCC 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 600
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BanII GRGCYC 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
Bbv12I GWGCWC 2 cut(s) 329, 600
BbvI GCAGC 1 cut(s) 778
BccI CCATC 2 cut(s) 172, 620
BciT130I CCWGG 3 cut(s) 396, 489, 825
BciVI GTATCC 2 cut(s) 678, 886
BcnI CCSGG 2 cut(s) 96, 132
BcoDI GTCTC 2 cut(s) 9, 129
BfaI CTAG 2 cut(s) 726, 776
BfrI CTTAAG 2 cut(s) 299, 506
BfuAI ACCTGC 1 cut(s) 455
BfuI GTATCC 2 cut(s) 678, 886
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 1 cut(s) 792
BlsI GCNGC 1 cut(s) 793
Bme1390I CCNGG 5 cut(s) 96, 132, 396, 489, 825
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 5 cut(s) 96, 132, 396, 489, 825
BmsI GCATC 4 cut(s) 51, 177, 878, 942
BoxI GACNNNNGTC 1 cut(s) 308
BpmI CTGGAG 3 cut(s) 825, 834, 846
Bpu14I TTCGAA 2 cut(s) 207, 216
BpuEI CTTGAG 1 cut(s) 1050
BpuMI CCSGG 2 cut(s) 96, 132
Bsa29I ATCGAT 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 2 cut(s) 50, 196
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 819, 849
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 101, 137, 314
Bse1I ACTGG 2 cut(s) 116, 817
Bse3DI GCAATG 2 cut(s) 675, 1086
BseBI CCWGG 3 cut(s) 396, 489, 825
BseCI ATCGAT 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 130
BseLI CCNNNNNNNGG 4 cut(s) 95, 101, 137, 314
BseMI GCAATG 2 cut(s) 675, 1086
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 2 cut(s) 116, 817
BseSI GKGCMC 1 cut(s) 600
BseXI GCAGC 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 164
BshVI ATCGAT 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 329, 600
BsiSI CCGG 4 cut(s) 51, 96, 132, 197
BslI CCNNNNNNNGG 4 cut(s) 95, 101, 137, 314
BsmAI GTCTC 2 cut(s) 9, 129
BsmBI CGTCTC 1 cut(s) 129
BsmI GAATGC 1 cut(s) 919
Bsp119I TTCGAA 2 cut(s) 207, 216
Bsp1286I GDGCHC 2 cut(s) 329, 600
Bsp143I GATC 3 cut(s) 71, 358, 1042
BspACI CCGC 5 cut(s) 164, 842, 851, 875, 999
BspCNI CTCAG 1 cut(s) 336
BspDI ATCGAT 1 cut(s) 263
BspFNI CGCG 1 cut(s) 164
BspHI TCATGA 2 cut(s) 412, 612
BspLI GGNNCC 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 1 cut(s) 66
BspQI GCTCTTC 1 cut(s) 897
BspT104I TTCGAA 2 cut(s) 207, 216
BspTI CTTAAG 2 cut(s) 299, 506
BsrDI GCAATG 2 cut(s) 675, 1086
BsrI ACTGG 2 cut(s) 116, 817
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 3 cut(s) 71, 358, 1042
BssNI GRCGYC 1 cut(s) 755
Bst2UI CCWGG 3 cut(s) 396, 489, 825
Bst4CI ACNGT 3 cut(s) 213, 383, 1058
Bst6I CTCTTC 2 cut(s) 230, 897
BstACI GRCGYC 1 cut(s) 755
BstAFI CTTAAG 2 cut(s) 299, 506
BstAPI GCANNNNNTGC 2 cut(s) 548, 788
BstBI TTCGAA 2 cut(s) 207, 216
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 3 cut(s) 344, 660, 956
BstFNI CGCG 1 cut(s) 164
BstKTI GATC 3 cut(s) 74, 361, 1045
BstMAI GTCTC 2 cut(s) 9, 129
BstMBI GATC 3 cut(s) 71, 358, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 191, 548, 788, 800, 848, 1088
BstNI CCWGG 3 cut(s) 396, 489, 825
BstNSI RCATGY 1 cut(s) 88
BstPAI GACNNNNGTC 1 cut(s) 308
BstSCI CCNGG 5 cut(s) 94, 130, 394, 487, 823
BstSLI GKGCMC 1 cut(s) 600
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 1 cut(s) 778
BstX2I RGATCY 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 1042
Bsu15I ATCGAT 1 cut(s) 263
BsuI GTATCC 2 cut(s) 678, 886
BsuTUI ATCGAT 1 cut(s) 263
BtgZI GCGATG 2 cut(s) 28, 200
BtsI GCAGTG 2 cut(s) 600, 786
BtsIMutI CAGTG 4 cut(s) 123, 447, 600, 786
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 192
CciI TCATGA 2 cut(s) 412, 612
Cfr13I GGNCC 1 cut(s) 146
ClaI ATCGAT 1 cut(s) 263
CseI GACGC 2 cut(s) 763, 1088
Csp6I GTAC 2 cut(s) 810, 1059
CspCI CAANNNNNGTGG 2 cut(s) 906, 941
CviAII CATG 8 cut(s) 85, 224, 413, 476, 613, 748, 820, 949
CviQI GTAC 2 cut(s) 810, 1059
DdeI CTNAG 3 cut(s) 344, 660, 956
DpnI GATC 3 cut(s) 73, 360, 1044
DpnII GATC 3 cut(s) 71, 358, 1042
Eam1104I CTCTTC 2 cut(s) 230, 897
EarI CTCTTC 2 cut(s) 230, 897
EciI GGCGGA 1 cut(s) 857
Ecl136II GAGCTC 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 329
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 6 cut(s) 47, 267, 471, 950, 1041, 1059
EcoICRI GAGCTC 1 cut(s) 327
EcoRII CCWGG 3 cut(s) 394, 487, 823
EcoT38I GRGCYC 1 cut(s) 329
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 8 cut(s) 88, 227, 416, 479, 616, 751, 823, 952
FalI AAGNNNNNCTT 4 cut(s) 282, 314, 664, 696
FatI CATG 8 cut(s) 84, 223, 412, 475, 612, 747, 819, 948
FauI CCCGC 1 cut(s) 868
Fnu4HI GCNGC 1 cut(s) 792
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 1 cut(s) 792
FspBI CTAG 2 cut(s) 726, 776
GluI GCNGC 1 cut(s) 792
GsuI CTGGAG 3 cut(s) 825, 834, 846
HapII CCGG 4 cut(s) 51, 96, 132, 197
HgaI GACGC 2 cut(s) 763, 1088
Hin1I GRCGYC 1 cut(s) 755
Hin1II CATG 8 cut(s) 88, 227, 416, 479, 616, 751, 823, 952
HincII GTYRAC 2 cut(s) 379, 1078
HindII GTYRAC 2 cut(s) 379, 1078
HinfI GANTC 5 cut(s) 31, 260, 304, 641, 816
HpaII CCGG 4 cut(s) 51, 96, 132, 197
HphI GGTGA 4 cut(s) 110, 467, 523, 763
Hpy166II GTNNAC 5 cut(s) 202, 242, 379, 598, 1078
Hpy188I TCNGA 3 cut(s) 345, 532, 1035
Hpy188III TCNNGA 6 cut(s) 64, 413, 470, 613, 650, 1067
Hpy8I GTNNAC 5 cut(s) 202, 242, 379, 598, 1078
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 6 cut(s) 242, 446, 883, 974, 984, 1016
HpyCH4III ACNGT 3 cut(s) 213, 383, 1058
HpyCH4IV ACGT 2 cut(s) 122, 882
HpyCH4V TGCA 8 cut(s) 190, 464, 542, 598, 715, 869, 917, 1091
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 548, 788, 800, 848, 1088
HpyF3I CTNAG 3 cut(s) 344, 660, 956
HpySE526I ACGT 2 cut(s) 122, 882
Hsp92I GRCGYC 1 cut(s) 755
Hsp92II CATG 8 cut(s) 88, 227, 416, 479, 616, 751, 823, 952
Kzo9I GATC 3 cut(s) 71, 358, 1042
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 2 cut(s) 199, 352
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 4 cut(s) 51, 177, 878, 942
MaeI CTAG 2 cut(s) 726, 776
MaeII ACGT 2 cut(s) 122, 882
MaeIII GTNAC 6 cut(s) 76, 98, 118, 154, 586, 751
MalI GATC 3 cut(s) 73, 360, 1044
MboI GATC 3 cut(s) 71, 358, 1042
MboII GAAGA 7 cut(s) 26, 40, 247, 283, 592, 914, 1052
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 2 cut(s) 329, 600
MluCI AATT 6 cut(s) 388, 617, 703, 737, 1027, 1093
MlyI GAGTC 3 cut(s) 298, 650, 825
MmeI TCCRAC 2 cut(s) 237, 1013
MnlI CCTC 4 cut(s) 646, 800, 821, 1063
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 5 cut(s) 125, 267, 300, 339, 507
MslI CAYNNNNRTG 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 875, 1001
MspCI CTTAAG 2 cut(s) 299, 506
MspI CCGG 4 cut(s) 51, 96, 132, 197
MspR9I CCNGG 5 cut(s) 96, 132, 396, 489, 825
Mva1269I GAATGC 1 cut(s) 919
MvaI CCWGG 3 cut(s) 396, 489, 825
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 6 cut(s) 191, 548, 788, 800, 848, 1088
NciI CCSGG 2 cut(s) 96, 132
NdeII GATC 3 cut(s) 71, 358, 1042
NlaIII CATG 8 cut(s) 88, 227, 416, 479, 616, 751, 823, 952
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 4 cut(s) 76, 98, 118, 751
NspI RCATGY 1 cut(s) 88
NspV TTCGAA 2 cut(s) 207, 216
PagI TCATGA 2 cut(s) 412, 612
PciSI GCTCTTC 1 cut(s) 897
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 919
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 31, 260
PflFI GACNNNGTC 1 cut(s) 1082
PkrI GCNGC 1 cut(s) 793
PleI GAGTC 3 cut(s) 298, 649, 824
PpsI GAGTC 3 cut(s) 298, 649, 824
PshAI GACNNNNGTC 1 cut(s) 308
Psp124BI GAGCTC 1 cut(s) 329
Psp6I CCWGG 3 cut(s) 394, 487, 823
PspGI CCWGG 3 cut(s) 394, 487, 823
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 1042
PsyI GACNNNGTC 1 cut(s) 1082
RsaI GTAC 2 cut(s) 811, 1060
RsaNI GTAC 2 cut(s) 810, 1059
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 329
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 5 cut(s) 125, 267, 300, 339, 507
SatI GCNGC 1 cut(s) 792
Sau3AI GATC 3 cut(s) 71, 358, 1042
Sau96I GGNCC 1 cut(s) 146
SchI GAGTC 3 cut(s) 298, 650, 825
ScrFI CCNGG 5 cut(s) 96, 132, 396, 489, 825
SduI GDGCHC 2 cut(s) 329, 600
SfaNI GCATC 4 cut(s) 51, 177, 878, 942
SfuI TTCGAA 2 cut(s) 207, 216
SinI GGWCC 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 3 cut(s) 299, 506, 1065
SmoI CTYRAG 3 cut(s) 299, 506, 1065
Sse9I AATT 6 cut(s) 388, 617, 703, 737, 1027, 1093
SsiI CCGC 5 cut(s) 164, 842, 851, 875, 999
SspMI CTAG 2 cut(s) 726, 776
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 5 cut(s) 94, 130, 394, 487, 823
TaaI ACNGT 3 cut(s) 213, 383, 1058
TaiI ACGT 2 cut(s) 125, 885
TaqI TCGA 4 cut(s) 207, 216, 263, 741
TasI AATT 6 cut(s) 388, 617, 703, 737, 1027, 1093
TfiI GAWTC 2 cut(s) 31, 260
Tru1I TTAA 5 cut(s) 125, 267, 300, 339, 507
Tru9I TTAA 5 cut(s) 125, 267, 300, 339, 507
TscAI CASTG 4 cut(s) 123, 454, 600, 793
TseFI GTSAC 4 cut(s) 76, 98, 118, 751
TseI GCWGC 1 cut(s) 791
Tsp45I GTSAC 4 cut(s) 76, 98, 118, 751
TspDTI ATGAA 5 cut(s) 212, 248, 492, 629, 702
TspGWI ACGGA 5 cut(s) 64, 117, 138, 153, 914
TspRI CASTG 4 cut(s) 123, 454, 600, 793
Tth111I GACNNNGTC 1 cut(s) 1082
Vha464I CTTAAG 2 cut(s) 299, 506
VneI GTGCAC 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 1 cut(s) 1093
XceI RCATGY 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 726, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.