Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005225

Physical Location & Seq

Genomic Coordinates Reverse (-)

26236534 .. 26240298

Gene Structure 3765 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1133600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGGCCCGCCGGACCCTCCCGATCTTTAAAAACCTCACACAGCGCCCCACGCCGGCACCATTCCTTCCCAAATCCCACCCGAACACCCGATCCGTCACCTACATGCCCCGACCCGGCGACGGTGCCCCGCGGCCCGTAACCCTGATCCCCGGCGATGGAATCGGCCCGCTTGTCACCAACGCCGTCGAGCAAGTCATGGAAGCGATGCACGCCCCCGTGTACTTCGAGCGCTACGACGTCCACGGCGACATGAAGCGGGTCCCGCCGGAGGTGATCGAGTCGATCCGGAAAAACAAGGTGTGCTTGAAGGGCGGGCTGAAGACTCCGGTGGGCGGCGGCGTGAGCTCGCTCAACGTGCAGCTGAGAAAAGAGCTCGACCTGTACGCTTCGCTAGTAAACTGCTTCAACCTCCCCGGCCTCCCCACCCGCCACGAGAACGTGGACATTGTCGTAATTAGAGAGAACACCGAGGGCGAATACGCCGGGCTCGAGCACGAGGTTGTCCCTGGCGTCGTTGAGAGCCTCAAGGTGATCACCAAGTTCTGCTCGGAGCGAATCGCGAAGTACGCTTTCGAGTATTCCTATTTGAACAACAGGAAGAAGGTGACTGCTGTGCACAAAGCCAACATTATGAAGCTGGCTGATGGGTTGTTCCTGGAGTCCTGCAGAGAGGTTGCTACAAAGTACCCAAGTATTAATTACAATGAAATCATTGTGGACAATTGCTGTATGCAACTCGTTTCGAAGCCCGAGCAATTTGATGTGATGGTCACTCCCAATCTTTATGGAAATCTAGTGGCAAATACGGCAGCTGGTATTGCTGGAGGCACTGGTGTCATGCCAGGAGGAAATGTGGGTGCTGATCACGCTGTTTTTGAGCAAGGTGCTTCGGCAGGAAATGTCGGAAATGAAAAGTTGTGGGAGCAAAAGAAGGCCAATCCAGTCGCTTTGCTTCTATCATCAGCAATGATGTTGAGGCATCTCCAGTTTCCTTCATTCGCTGATCGACTTGAAACAGCGGTGAAGCGAGTAATATTAGAAGGCAAGTACCGGACAAAAGATCTCGGAGGAGTCAGCACTACCCAAGAGGTCGTTGATGCCGTAATTGGTAAACTGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006102 GO:0008150 GO:0008152 GO:0008270 GO:0009060 GO:0009987 GO:0015980 GO:0016999 GO:0017144 GO:0019752 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046872 GO:0046914 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00020 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

374

Amino Acids

40.78

Weight (kDa)

8.59

Isoelectric Point (pI)

38.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	46 - 368	4e-79	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17130 AT2G17130 AT4G35260
fragaria_vesca	FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520
malus_domestica	MD05G1133600.v1.1 MD10G1138600.v1.1
prunus_persica	Prupe.3G128300_v2.0.a1 Prupe.8G177400_v2.0.a1 Prupe.8G177400_v2.0.a1 Prupe.8G177400_v2.0.a1
pyrus_communis	pycom07g23620
rosa_chinensis	RchiOBHm_Chr6g0280541
rosa_laevigata	RLG00000013056
rosa_multiflora	Rmu_co8141332.1_g000001 Rmu_sc0005585.1_g000001 Rmu_sc0015818.1_g000005
rosa_roxburghii	Rroxscaffold_7G00188000
rosa_rugosa	Rorug06G0131900 Rorug06G0132000
rosa_samantha	Rh6AG244400 Rh6BG246800 Rh6CG248200 Rh6DG239600
rosa_wichuraiana	Rw6G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	240
AccB1I	GGYRCC	2 cut(s)	55, 122
AccII	CGCG	2 cut(s)	130, 560
AccIII	TCCGGA	1 cut(s)	285
AclWI	GGATC	3 cut(s)	84, 139, 277
AcuI	CTGAAG	1 cut(s)	338
AcyI	GRCGYC	2 cut(s)	237, 510
AfaI	GTAC	5 cut(s)	221, 383, 566, 686, 1049
AfeI	AGCGCT	1 cut(s)	230
AfiI	CCNNNNNNNGG	6 cut(s)	52, 113, 119, 155, 268, 332
AgsI	TTSAA	4 cut(s)	307, 406, 589, 1013
AjnI	CCWGG	3 cut(s)	505, 654, 841
AloI	GAACNNNNNNTCC	2 cut(s)	74, 106
AluBI	AGCT	5 cut(s)	345, 361, 373, 637, 812
AluI	AGCT	5 cut(s)	345, 361, 373, 637, 812
Alw21I	GWGCWC	4 cut(s)	347, 375, 495, 618
Alw44I	GTGCAC	1 cut(s)	614
AlwI	GGATC	3 cut(s)	84, 139, 277
Ama87I	CYCGRG	2 cut(s)	488, 749
Aor13HI	TCCGGA	1 cut(s)	285
Aor51HI	AGCGCT	1 cut(s)	230
AoxI	GGCC	5 cut(s)	3, 131, 163, 415, 933
ApaLI	GTGCAC	1 cut(s)	614
ApeKI	GCWGC	2 cut(s)	358, 809
AseI	ATTAAT	1 cut(s)	696
AspLEI	GCGC	2 cut(s)	45, 231
AspS9I	GGNCC	5 cut(s)	4, 12, 132, 164, 259
AsuC2I	CCSGG	4 cut(s)	114, 150, 414, 484
AsuHPI	GGTGA	7 cut(s)	88, 166, 283, 526, 541, 616, 1033
AsuII	TTCGAA	1 cut(s)	743
AvaI	CYCGRG	2 cut(s)	488, 749
AvaII	GGWCC	2 cut(s)	12, 259
BaeGI	GKGCMC	2 cut(s)	127, 618
BanI	GGYRCC	2 cut(s)	55, 122
BanII	GRGCYC	3 cut(s)	347, 375, 489
BauI	CACGAG	2 cut(s)	431, 494
BbsI	GAAGAC	1 cut(s)	326
Bbv12I	GWGCWC	4 cut(s)	347, 375, 495, 618
BbvI	GCAGC	2 cut(s)	370, 821
BccI	CCATC	3 cut(s)	149, 638, 760
BceAI	ACGGC	4 cut(s)	167, 259, 822, 1085
BciT130I	CCWGG	3 cut(s)	507, 656, 843
BclI	TGATCA	2 cut(s)	531, 862
BcnI	CCSGG	4 cut(s)	114, 150, 414, 484
BfaI	CTAG	2 cut(s)	392, 794
BfmI	CTRYAG	1 cut(s)	664
BfoI	RGCGCY	2 cut(s)	46, 232
BglII	AGATCT	1 cut(s)	1060
BisI	GCNGC	5 cut(s)	131, 334, 337, 359, 810
BlsI	GCNGC	5 cut(s)	132, 335, 338, 360, 811
Bme1390I	CCNGG	7 cut(s)	114, 150, 414, 484, 507, 656, 843
Bme18I	GGWCC	2 cut(s)	12, 259
BmeT110I	CYCGRG	2 cut(s)	488, 749
BmgT120I	GGNCC	5 cut(s)	4, 12, 132, 164, 259
BmiI	GGNNCC	5 cut(s)	14, 57, 124, 260, 261
BmrFI	CCNGG	7 cut(s)	114, 150, 414, 484, 507, 656, 843
BmsI	GCATC	3 cut(s)	195, 988, 1087
BpiI	GAAGAC	1 cut(s)	326
BpmI	CTGGAG	3 cut(s)	677, 843, 968
Bpu14I	TTCGAA	1 cut(s)	743
BpuEI	CTTGAG	1 cut(s)	509
BpuMI	CCSGG	4 cut(s)	114, 150, 414, 484
BsaHI	GRCGYC	2 cut(s)	237, 510
BsaJI	CCNNGG	6 cut(s)	128, 148, 241, 412, 468, 505
BsaWI	WCCGGW	3 cut(s)	285, 325, 1050
BsaXI	ACNNNNNCTCC	2 cut(s)	837, 867
Bsc4I	CCNNNNNNNGG	6 cut(s)	52, 113, 119, 155, 268, 332
Bse118I	RCCGGY	1 cut(s)	52
Bse1I	ACTGG	4 cut(s)	835, 941, 985, 1119
Bse3DI	GCAATG	1 cut(s)	972
BseAI	TCCGGA	1 cut(s)	285
BseBI	CCWGG	3 cut(s)	507, 656, 843
BseDI	CCNNGG	6 cut(s)	128, 148, 241, 412, 468, 505
BseLI	CCNNNNNNNGG	6 cut(s)	52, 113, 119, 155, 268, 332
BseMI	GCAATG	1 cut(s)	972
BseMII	CTCAG	1 cut(s)	353
BseNI	ACTGG	4 cut(s)	835, 941, 985, 1119
BseRI	GAGGAG	1 cut(s)	1083
BseSI	GKGCMC	2 cut(s)	127, 618
BseXI	GCAGC	2 cut(s)	370, 821
BsgI	GTGCAG	1 cut(s)	377
Bsh1236I	CGCG	2 cut(s)	130, 560
BshFI	GGCC	5 cut(s)	5, 133, 165, 417, 935
BshNI	GGYRCC	2 cut(s)	55, 122
BsiHKAI	GWGCWC	4 cut(s)	347, 375, 495, 618
BsiHKCI	CYCGRG	2 cut(s)	488, 749
BslFI	GGGAC	2 cut(s)	245, 488
BslI	CCNNNNNNNGG	6 cut(s)	52, 113, 119, 155, 268, 332
BsmFI	GGGAC	2 cut(s)	245, 488
BsnI	GGCC	5 cut(s)	5, 133, 165, 417, 935
BsoBI	CYCGRG	2 cut(s)	488, 749
Bsp119I	TTCGAA	1 cut(s)	743
Bsp1286I	GDGCHC	6 cut(s)	127, 347, 375, 489, 495, 618
Bsp13I	TCCGGA	1 cut(s)	285
Bsp143I	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
Bsp68I	TCGCGA	1 cut(s)	560
BspANI	GGCC	5 cut(s)	5, 133, 165, 417, 935
BspCNI	CTCAG	1 cut(s)	354
BspEI	TCCGGA	1 cut(s)	285
BspFNI	CGCG	2 cut(s)	130, 560
BspLI	GGNNCC	5 cut(s)	14, 57, 124, 260, 261
BspMAI	CTGCAG	1 cut(s)	668
BspPI	GGATC	3 cut(s)	84, 139, 277
BspT104I	TTCGAA	1 cut(s)	743
BspT107I	GGYRCC	2 cut(s)	55, 122
BsrDI	GCAATG	1 cut(s)	972
BsrFI	RCCGGY	1 cut(s)	52
BsrI	ACTGG	4 cut(s)	835, 941, 985, 1119
BssAI	RCCGGY	1 cut(s)	52
BssECI	CCNNGG	6 cut(s)	128, 148, 241, 412, 468, 505
BssMI	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
BssNI	GRCGYC	2 cut(s)	237, 510
BssSI	CACGAG	2 cut(s)	431, 494
Bst2BI	CACGAG	2 cut(s)	431, 494
Bst2UI	CCWGG	3 cut(s)	507, 656, 843
Bst4CI	ACNGT	1 cut(s)	122
BstACI	GRCGYC	2 cut(s)	237, 510
BstBI	TTCGAA	1 cut(s)	743
BstC8I	GCNNGC	7 cut(s)	7, 54, 167, 210, 314, 347, 639
BstDEI	CTNAG	1 cut(s)	362
BstDSI	CCRYGG	2 cut(s)	128, 241
BstFNI	CGCG	2 cut(s)	130, 560
BstH2I	RGCGCY	2 cut(s)	46, 232
BstHHI	GCGC	2 cut(s)	45, 231
BstKTI	GATC	9 cut(s)	24, 92, 147, 276, 285, 534, 865, 1006, 1063
BstMBI	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
BstNI	CCWGG	3 cut(s)	507, 656, 843
BstNSI	RCATGY	1 cut(s)	106
BstSCI	CCNGG	7 cut(s)	112, 148, 412, 482, 505, 654, 841
BstSFI	CTRYAG	1 cut(s)	664
BstSLI	GKGCMC	2 cut(s)	127, 618
BstUI	CGCG	2 cut(s)	130, 560
BstV1I	GCAGC	2 cut(s)	370, 821
BstV2I	GAAGAC	1 cut(s)	326
BstX2I	RGATCY	1 cut(s)	1060
BstYI	RGATCY	1 cut(s)	1060
BsuRI	GGCC	5 cut(s)	5, 133, 165, 417, 935
BtgI	CCRYGG	2 cut(s)	128, 241
BtgZI	GCGATG	2 cut(s)	168, 218
BtsIMutI	CAGTG	1 cut(s)	828
BtuMI	TCGCGA	1 cut(s)	560
Cac8I	GCNNGC	7 cut(s)	7, 54, 167, 210, 314, 347, 639
CfoI	GCGC	2 cut(s)	45, 231
Cfr10I	RCCGGY	1 cut(s)	52
Cfr13I	GGNCC	5 cut(s)	4, 12, 132, 164, 259
Cfr42I	CCGCGG	1 cut(s)	131
CseI	GACGC	1 cut(s)	499
Csp6I	GTAC	5 cut(s)	220, 382, 565, 685, 1048
CviAII	CATG	4 cut(s)	103, 196, 250, 838
CviQI	GTAC	5 cut(s)	220, 382, 565, 685, 1048
DdeI	CTNAG	1 cut(s)	362
DpnI	GATC	9 cut(s)	23, 91, 146, 275, 284, 533, 864, 1005, 1062
DpnII	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
DraI	TTTAAA	1 cut(s)	28
Ecl136II	GAGCTC	2 cut(s)	345, 373
Eco24I	GRGCYC	3 cut(s)	347, 375, 489
Eco47I	GGWCC	2 cut(s)	12, 259
Eco47III	AGCGCT	1 cut(s)	230
Eco53kI	GAGCTC	2 cut(s)	345, 373
Eco57I	CTGAAG	1 cut(s)	338
Eco88I	CYCGRG	2 cut(s)	488, 749
EcoICRI	GAGCTC	2 cut(s)	345, 373
EcoO109I	RGGNCCY	1 cut(s)	259
EcoRII	CCWGG	3 cut(s)	505, 654, 841
EcoT38I	GRGCYC	3 cut(s)	347, 375, 489
FaeI	CATG	4 cut(s)	106, 199, 253, 841
FaiI	YATR	7 cut(s)	104, 197, 251, 632, 731, 786, 839
FalI	AAGNNNNNCTT	2 cut(s)	287, 319
FaqI	GGGAC	2 cut(s)	245, 488
FatI	CATG	4 cut(s)	102, 195, 249, 837
FauI	CCCGC	7 cut(s)	14, 135, 174, 249, 270, 305, 434
FbaI	TGATCA	2 cut(s)	531, 862
Fnu4HI	GCNGC	5 cut(s)	131, 334, 337, 359, 810
FriOI	GRGCYC	3 cut(s)	347, 375, 489
Fsp4HI	GCNGC	5 cut(s)	131, 334, 337, 359, 810
FspBI	CTAG	2 cut(s)	392, 794
GlaI	GCGC	2 cut(s)	44, 230
GluI	GCNGC	5 cut(s)	131, 334, 337, 359, 810
GsuI	CTGGAG	3 cut(s)	677, 843, 968
HaeII	RGCGCY	2 cut(s)	46, 232
HaeIII	GGCC	5 cut(s)	5, 133, 165, 417, 935
HgaI	GACGC	1 cut(s)	499
HhaI	GCGC	2 cut(s)	45, 231
Hin1I	GRCGYC	2 cut(s)	237, 510
Hin1II	CATG	4 cut(s)	106, 199, 253, 841
Hin6I	GCGC	2 cut(s)	43, 229
HinP1I	GCGC	2 cut(s)	43, 229
HinfI	GANTC	6 cut(s)	159, 278, 322, 555, 659, 1071
HphI	GGTGA	7 cut(s)	88, 166, 283, 526, 541, 616, 1033
Hpy166II	GTNNAC	7 cut(s)	220, 241, 397, 442, 616, 718, 1112
Hpy188I	TCNGA	3 cut(s)	550, 905, 1067
Hpy188III	TCNNGA	3 cut(s)	19, 286, 559
Hpy8I	GTNNAC	7 cut(s)	220, 241, 397, 442, 616, 718, 1112
Hpy99I	CGWCG	4 cut(s)	122, 188, 239, 515
HpyAV	CCTTC	6 cut(s)	74, 301, 595, 925, 1002, 1034
HpyCH4III	ACNGT	1 cut(s)	122
HpyCH4IV	ACGT	3 cut(s)	237, 354, 438
HpyCH4V	TGCA	5 cut(s)	208, 358, 616, 666, 733
HpyF3I	CTNAG	1 cut(s)	362
HpySE526I	ACGT	3 cut(s)	237, 354, 438
Hsp92I	GRCGYC	2 cut(s)	237, 510
Hsp92II	CATG	4 cut(s)	106, 199, 253, 841
HspAI	GCGC	2 cut(s)	43, 229
KflI	GGGWCCC	1 cut(s)	259
Kpn2I	TCCGGA	1 cut(s)	285
KroI	GCCGGC	1 cut(s)	52
KroNI	GCCGGC	1 cut(s)	54
Ksp22I	TGATCA	2 cut(s)	531, 862
KspI	CCGCGG	1 cut(s)	131
Kzo9I	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
LmnI	GCTCC	2 cut(s)	550, 922
Lsp1109I	GCAGC	2 cut(s)	370, 821
LweI	GCATC	3 cut(s)	195, 988, 1087
MaeI	CTAG	2 cut(s)	392, 794
MaeII	ACGT	3 cut(s)	237, 354, 438
MaeIII	GTNAC	5 cut(s)	94, 136, 172, 604, 769
MalI	GATC	9 cut(s)	23, 91, 146, 275, 284, 533, 864, 1005, 1062
MboI	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
MboII	GAAGA	2 cut(s)	331, 610
MfeI	CAATTG	1 cut(s)	721
MflI	RGATCY	1 cut(s)	1060
MhlI	GDGCHC	6 cut(s)	127, 347, 375, 489, 495, 618
MluCI	AATT	5 cut(s)	453, 697, 721, 755, 1104
MlyI	GAGTC	4 cut(s)	287, 316, 668, 1080
MmeI	TCCRAC	1 cut(s)	883
MroI	TCCGGA	1 cut(s)	285
MroNI	GCCGGC	1 cut(s)	52
MseI	TTAA	2 cut(s)	27, 696
MslI	CAYNNNNRTG	1 cut(s)	101
MspA1I	CMGCKG	4 cut(s)	130, 361, 812, 1019
MspR9I	CCNGG	7 cut(s)	114, 150, 414, 484, 507, 656, 843
MunI	CAATTG	1 cut(s)	721
MvaI	CCWGG	3 cut(s)	507, 656, 843
MvnI	CGCG	2 cut(s)	130, 560
NaeI	GCCGGC	1 cut(s)	54
NciI	CCSGG	4 cut(s)	114, 150, 414, 484
NdeII	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
NgoMIV	GCCGGC	1 cut(s)	52
NlaIII	CATG	4 cut(s)	106, 199, 253, 841
NlaIV	GGNNCC	5 cut(s)	14, 57, 124, 260, 261
NmuCI	GTSAC	4 cut(s)	94, 172, 604, 769
NruI	TCGCGA	1 cut(s)	560
NspI	RCATGY	1 cut(s)	106
NspV	TTCGAA	1 cut(s)	743
PaeR7I	CTCGAG	1 cut(s)	488
PcsI	WCGNNNNNNNCGW	4 cut(s)	231, 243, 486, 1098
PdiI	GCCGGC	1 cut(s)	54
PfeI	GAWTC	2 cut(s)	159, 555
PflFI	GACNNNGTC	1 cut(s)	446
PfoI	TCCNGGA	1 cut(s)	654
PkrI	GCNGC	5 cut(s)	132, 335, 338, 360, 811
PleI	GAGTC	4 cut(s)	286, 316, 667, 1079
PpsI	GAGTC	4 cut(s)	286, 316, 667, 1079
PpuMI	RGGWCCY	1 cut(s)	259
PshBI	ATTAAT	1 cut(s)	696
Psp124BI	GAGCTC	2 cut(s)	347, 375
Psp5II	RGGWCCY	1 cut(s)	259
Psp6I	CCWGG	3 cut(s)	505, 654, 841
PspGI	CCWGG	3 cut(s)	505, 654, 841
PspN4I	GGNNCC	5 cut(s)	14, 57, 124, 260, 261
PspPI	GGNCC	5 cut(s)	4, 12, 132, 164, 259
PspPPI	RGGWCCY	1 cut(s)	259
PspXI	VCTCGAGB	1 cut(s)	488
PstI	CTGCAG	1 cut(s)	668
PsuI	RGATCY	1 cut(s)	1060
PsyI	GACNNNGTC	1 cut(s)	446
PvuII	CAGCTG	2 cut(s)	361, 812
RruI	TCGCGA	1 cut(s)	560
RsaI	GTAC	5 cut(s)	221, 383, 566, 686, 1049
RsaNI	GTAC	5 cut(s)	220, 382, 565, 685, 1048
RseI	CAYNNNNRTG	1 cut(s)	101
SacI	GAGCTC	2 cut(s)	347, 375
SacII	CCGCGG	1 cut(s)	131
SaqAI	TTAA	2 cut(s)	27, 696
SatI	GCNGC	5 cut(s)	131, 334, 337, 359, 810
Sau3AI	GATC	9 cut(s)	21, 89, 144, 273, 282, 531, 862, 1003, 1060
Sau96I	GGNCC	5 cut(s)	4, 12, 132, 164, 259
SchI	GAGTC	4 cut(s)	287, 316, 668, 1080
ScrFI	CCNGG	7 cut(s)	114, 150, 414, 484, 507, 656, 843
SduI	GDGCHC	6 cut(s)	127, 347, 375, 489, 495, 618
SfaNI	GCATC	3 cut(s)	195, 988, 1087
SfcI	CTRYAG	1 cut(s)	664
Sfr274I	CTCGAG	1 cut(s)	488
Sfr303I	CCGCGG	1 cut(s)	131
SfuI	TTCGAA	1 cut(s)	743
SgrBI	CCGCGG	1 cut(s)	131
SinI	GGWCC	2 cut(s)	12, 259
SlaI	CTCGAG	1 cut(s)	488
SmiMI	CAYNNNNRTG	1 cut(s)	101
SmlI	CTYRAG	2 cut(s)	488, 524
SmoI	CTYRAG	2 cut(s)	488, 524
Sse9I	AATT	5 cut(s)	453, 697, 721, 755, 1104
SspI	AATATT	1 cut(s)	1035
SspMI	CTAG	2 cut(s)	392, 794
SstI	GAGCTC	2 cut(s)	347, 375
StyD4I	CCNGG	7 cut(s)	112, 148, 412, 482, 505, 654, 841
TaaI	ACNGT	1 cut(s)	122
TaiI	ACGT	3 cut(s)	240, 357, 441
TaqI	TCGA	9 cut(s)	186, 225, 276, 281, 375, 489, 573, 743, 1006
TasI	AATT	5 cut(s)	453, 697, 721, 755, 1104
TatI	WGTACW	1 cut(s)	219
TauI	GCSGC	3 cut(s)	133, 336, 339
TfiI	GAWTC	2 cut(s)	159, 555
Tru1I	TTAA	2 cut(s)	27, 696
Tru9I	TTAA	2 cut(s)	27, 696
TscAI	CASTG	1 cut(s)	835
TseFI	GTSAC	4 cut(s)	94, 172, 604, 769
TseI	GCWGC	2 cut(s)	358, 809
Tsp45I	GTSAC	4 cut(s)	94, 172, 604, 769
TspDTI	ATGAA	5 cut(s)	266, 647, 720, 924, 984
TspGWI	ACGGA	1 cut(s)	82
TspRI	CASTG	1 cut(s)	835
Tth111I	GACNNNGTC	1 cut(s)	446
VneI	GTGCAC	1 cut(s)	614
VpaK11BI	GGWCC	2 cut(s)	12, 259
VspI	ATTAAT	1 cut(s)	696
XceI	RCATGY	1 cut(s)	106
XhoI	CTCGAG	1 cut(s)	488
XspI	CTAG	2 cut(s)	392, 794
ZraI	GACGTC	1 cut(s)	238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.