Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0005225

Physical Location & Seq

Genomic Coordinates Reverse (-)

43331828 .. 43335289

Gene Structure 3462 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG244400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 786 bp

ATGGCCCGTCGAGCCCTCCCCATCCTCAAGCAGTTCTCTCACCGCCCCACACTCGGATCCTTCCTCCCCAAACCCGACCCGAACACCCGATCGGTCACTTACATGCCCCGACCCGGCGACGGAACCCCGCGCTGCGTCACTCTCATCCCCGGCGACGGGATCGGGCCGCTCGTCACCAACGCCGTCGAGCAAGTCATGGAGGCGATGCACGCGCCGGTGTTCTTCGAGCGCTACGACGTCCACGGCGACATGAGCCGCGTGCCGCCGGAGGTGATGGACTCGATCCGGAAGAACAAGGTCTGCATAAAGGGCGGGCTTAAGACGCCGGTCGGCGGCGGTGTGAGCTCGCTCAATATGCAGCTGAGGAAGGAGCTTGATTTGTACGCGTCGCTGGTCAATTGCTTCAACCTCCCTGGACTCCCCACGCGCCACGAGAACGTGGACATTGTGGTGATCAGAGAGAACACCGAGGGTGAGTACGCCGGCCTCGAGCACGAAGTCGTTCCCGGAGTCGTGGAGAGCCTCAAGGTGATCACAAAGTTCTGCTCAGAACGCATAGCCAAGTATGCGTTTGAGTATGCATACTTGAATAACAGAAAGAAAGTGACTGCCGTGCACAAAGCCAACATCATGAAGCTTGCGGATGGTTTGTTCTTGGAGTCTTGCCGGGAGGTTGCGACAAAGTATCCCAGTATCAAGTATAATGAGATTATCGTGGACAATTGCTGTATGCAACTTGTTTCAAAGCCTGAACAGTTTGATGTGATGGTATGCGAGCGCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006102 GO:0008150 GO:0008152 GO:0008270 GO:0009060 GO:0009987 GO:0015980 GO:0016999 GO:0017144 GO:0019752 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046872 GO:0046914 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00020 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

261

Amino Acids

29.18

Weight (kDa)

8.36

Isoelectric Point (pI)

42.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	46 - 259	2.5e-51	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17130 AT2G17130 AT4G35260
fragaria_vesca	FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520
malus_domestica	MD05G1133600.v1.1 MD10G1138600.v1.1
prunus_persica	Prupe.3G128300_v2.0.a1 Prupe.8G177400_v2.0.a1 Prupe.8G177400_v2.0.a1 Prupe.8G177400_v2.0.a1
pyrus_communis	pycom07g23620
rosa_chinensis	RchiOBHm_Chr6g0280541
rosa_laevigata	RLG00000013056
rosa_multiflora	Rmu_co8141332.1_g000001 Rmu_sc0005585.1_g000001 Rmu_sc0015818.1_g000005
rosa_roxburghii	Rroxscaffold_7G00188000
rosa_rugosa	Rorug06G0131900 Rorug06G0132000
rosa_samantha	Rh6AG244400 Rh6BG246800 Rh6CG248200 Rh6DG239600
rosa_wichuraiana	Rw6G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	240
AccBSI	CCGCTC	1 cut(s)	169
AccII	CGCG	5 cut(s)	130, 212, 258, 386, 427
AccIII	TCCGGA	1 cut(s)	285
AciI	CCGC	9 cut(s)	43, 128, 167, 256, 263, 312, 333, 336, 641
AclWI	GGATC	4 cut(s)	51, 64, 167, 277
AcyI	GRCGYC	2 cut(s)	237, 323
AfaI	GTAC	2 cut(s)	383, 479
AfeI	AGCGCT	1 cut(s)	230
AfiI	CCNNNNNNNGG	6 cut(s)	53, 113, 119, 155, 156, 332
AflII	CTTAAG	1 cut(s)	317
AflIII	ACRYGT	1 cut(s)	384
AgsI	TTSAA	3 cut(s)	406, 589, 744
AjnI	CCWGG	1 cut(s)	412
AloI	GAACNNNNNNTCC	2 cut(s)	635, 667
AluBI	AGCT	4 cut(s)	345, 361, 373, 637
AluI	AGCT	4 cut(s)	345, 361, 373, 637
Alw21I	GWGCWC	3 cut(s)	347, 495, 618
Alw44I	GTGCAC	1 cut(s)	614
AlwI	GGATC	4 cut(s)	51, 64, 167, 277
Ama87I	CYCGRG	1 cut(s)	488
Aor13HI	TCCGGA	1 cut(s)	285
Aor51HI	AGCGCT	1 cut(s)	230
AoxI	GGCC	3 cut(s)	3, 164, 484
ApaLI	GTGCAC	1 cut(s)	614
ApeKI	GCWGC	2 cut(s)	132, 358
Asp700I	GAANNNNTTC	1 cut(s)	501
AspLEI	GCGC	5 cut(s)	132, 214, 231, 429, 780
AspS9I	GGNCC	2 cut(s)	4, 164
AsuC2I	CCSGG	4 cut(s)	114, 150, 507, 668
AsuHPI	GGTGA	6 cut(s)	32, 166, 283, 463, 485, 541
AvaI	CYCGRG	1 cut(s)	488
BaeGI	GKGCMC	1 cut(s)	618
BamHI	GGATCC	1 cut(s)	56
BanII	GRGCYC	2 cut(s)	16, 347
BauI	CACGAG	1 cut(s)	431
Bbv12I	GWGCWC	3 cut(s)	347, 495, 618
BbvCI	CCTCAGC	1 cut(s)	362
BbvI	GCAGC	2 cut(s)	119, 370
BccI	CCATC	4 cut(s)	29, 268, 638, 760
BceAI	ACGGC	3 cut(s)	167, 259, 596
BciT130I	CCWGG	1 cut(s)	414
BciVI	GTATCC	1 cut(s)	696
BclI	TGATCA	2 cut(s)	453, 531
BcnI	CCSGG	4 cut(s)	114, 150, 507, 668
BfoI	RGCGCY	1 cut(s)	232
BfrI	CTTAAG	1 cut(s)	317
BfuI	GTATCC	1 cut(s)	696
BisI	GCNGC	6 cut(s)	133, 167, 256, 263, 334, 359
BlsI	GCNGC	6 cut(s)	134, 168, 257, 264, 335, 360
Bme1390I	CCNGG	5 cut(s)	114, 150, 414, 507, 668
BmeT110I	CYCGRG	1 cut(s)	488
BmgT120I	GGNCC	2 cut(s)	4, 164
BmiI	GGNNCC	2 cut(s)	58, 124
BmrFI	CCNGG	5 cut(s)	114, 150, 414, 507, 668
BmrI	ACTGGG	1 cut(s)	684
BmsI	GCATC	1 cut(s)	195
BmuI	ACTGGG	1 cut(s)	684
BoxI	GACNNNNGTC	1 cut(s)	326
Bpu10I	CCTNAGC	1 cut(s)	362
BpuEI	CTTGAG	2 cut(s)	11, 509
BpuMI	CCSGG	4 cut(s)	114, 150, 507, 668
BsaHI	GRCGYC	2 cut(s)	237, 323
BsaJI	CCNNGG	4 cut(s)	148, 241, 412, 468
BsaWI	WCCGGW	1 cut(s)	285
Bsc4I	CCNNNNNNNGG	6 cut(s)	53, 113, 119, 155, 156, 332
Bse118I	RCCGGY	3 cut(s)	214, 325, 482
Bse1I	ACTGG	1 cut(s)	690
BseAI	TCCGGA	1 cut(s)	285
BseBI	CCWGG	1 cut(s)	414
BseDI	CCNNGG	4 cut(s)	148, 241, 412, 468
BseGI	GGATG	3 cut(s)	21, 144, 649
BseLI	CCNNNNNNNGG	6 cut(s)	53, 113, 119, 155, 156, 332
BseMII	CTCAG	2 cut(s)	353, 561
BseNI	ACTGG	1 cut(s)	690
BseSI	GKGCMC	1 cut(s)	618
BseXI	GCAGC	2 cut(s)	119, 370
Bsh1236I	CGCG	5 cut(s)	130, 212, 258, 386, 427
Bsh1285I	CGRYCG	2 cut(s)	92, 330
BshFI	GGCC	3 cut(s)	5, 166, 486
BsiEI	CGRYCG	2 cut(s)	92, 330
BsiHKAI	GWGCWC	3 cut(s)	347, 495, 618
BsiHKCI	CYCGRG	1 cut(s)	488
BsiSI	CCGG	9 cut(s)	114, 150, 215, 266, 286, 326, 483, 507, 667
BslI	CCNNNNNNNGG	6 cut(s)	53, 113, 119, 155, 156, 332
BsnI	GGCC	3 cut(s)	5, 166, 486
BsoBI	CYCGRG	1 cut(s)	488
Bsp1286I	GDGCHC	4 cut(s)	16, 347, 495, 618
Bsp13I	TCCGGA	1 cut(s)	285
Bsp143I	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
BspACI	CCGC	9 cut(s)	43, 128, 167, 256, 263, 312, 333, 336, 641
BspANI	GGCC	3 cut(s)	5, 166, 486
BspCNI	CTCAG	2 cut(s)	354, 560
BspEI	TCCGGA	1 cut(s)	285
BspFNI	CGCG	5 cut(s)	130, 212, 258, 386, 427
BspHI	TCATGA	1 cut(s)	630
BspLI	GGNNCC	2 cut(s)	58, 124
BspPI	GGATC	4 cut(s)	51, 64, 167, 277
BspTI	CTTAAG	1 cut(s)	317
BsrBI	CCGCTC	1 cut(s)	169
BsrFI	RCCGGY	3 cut(s)	214, 325, 482
BsrI	ACTGG	1 cut(s)	690
BssAI	RCCGGY	3 cut(s)	214, 325, 482
BssECI	CCNNGG	4 cut(s)	148, 241, 412, 468
BssMI	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
BssNI	GRCGYC	2 cut(s)	237, 323
BssSI	CACGAG	1 cut(s)	431
Bst2BI	CACGAG	1 cut(s)	431
Bst2UI	CCWGG	1 cut(s)	414
Bst4CI	ACNGT	1 cut(s)	756
BstACI	GRCGYC	2 cut(s)	237, 323
BstAFI	CTTAAG	1 cut(s)	317
BstC8I	GCNNGC	7 cut(s)	210, 260, 314, 347, 484, 639, 776
BstDEI	CTNAG	2 cut(s)	362, 547
BstDSI	CCRYGG	1 cut(s)	241
BstF5I	GGATG	3 cut(s)	21, 144, 649
BstFNI	CGCG	5 cut(s)	130, 212, 258, 386, 427
BstH2I	RGCGCY	1 cut(s)	232
BstHHI	GCGC	5 cut(s)	132, 214, 231, 429, 780
BstKTI	GATC	6 cut(s)	59, 92, 162, 285, 456, 534
BstMBI	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
BstMCI	CGRYCG	2 cut(s)	92, 330
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 209, 252, 309, 322, 342, 355, 552, 566
BstNI	CCWGG	1 cut(s)	414
BstNSI	RCATGY	1 cut(s)	106
BstPAI	GACNNNNGTC	1 cut(s)	326
BstSCI	CCNGG	5 cut(s)	112, 148, 412, 505, 666
BstSLI	GKGCMC	1 cut(s)	618
BstUI	CGCG	5 cut(s)	130, 212, 258, 386, 427
BstV1I	GCAGC	2 cut(s)	119, 370
BstX2I	RGATCY	1 cut(s)	56
BstYI	RGATCY	1 cut(s)	56
BsuI	GTATCC	1 cut(s)	696
BsuRI	GGCC	3 cut(s)	5, 166, 486
BtgI	CCRYGG	1 cut(s)	241
BtgZI	GCGATG	1 cut(s)	218
BtsCI	GGATG	3 cut(s)	21, 144, 649
Cac8I	GCNNGC	7 cut(s)	210, 260, 314, 347, 484, 639, 776
CciI	TCATGA	1 cut(s)	630
CfoI	GCGC	5 cut(s)	132, 214, 231, 429, 780
Cfr10I	RCCGGY	3 cut(s)	214, 325, 482
Cfr13I	GGNCC	2 cut(s)	4, 164
CseI	GACGC	3 cut(s)	124, 331, 375
Csp6I	GTAC	2 cut(s)	382, 478
CviAII	CATG	4 cut(s)	103, 196, 250, 631
CviQI	GTAC	2 cut(s)	382, 478
DdeI	CTNAG	2 cut(s)	362, 547
DpnI	GATC	6 cut(s)	58, 91, 161, 284, 455, 533
DpnII	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
Ecl136II	GAGCTC	1 cut(s)	345
Eco24I	GRGCYC	2 cut(s)	16, 347
Eco47III	AGCGCT	1 cut(s)	230
Eco53kI	GAGCTC	1 cut(s)	345
Eco88I	CYCGRG	1 cut(s)	488
EcoICRI	GAGCTC	1 cut(s)	345
EcoRII	CCWGG	1 cut(s)	412
EcoT22I	ATGCAT	1 cut(s)	583
EcoT38I	GRGCYC	2 cut(s)	16, 347
FaeI	CATG	4 cut(s)	106, 199, 253, 634
FatI	CATG	4 cut(s)	102, 195, 249, 630
FauI	CCCGC	2 cut(s)	135, 305
FbaI	TGATCA	2 cut(s)	453, 531
Fnu4HI	GCNGC	6 cut(s)	133, 167, 256, 263, 334, 359
FokI	GGATG	3 cut(s)	8, 131, 656
FriOI	GRGCYC	2 cut(s)	16, 347
Fsp4HI	GCNGC	6 cut(s)	133, 167, 256, 263, 334, 359
GlaI	GCGC	5 cut(s)	131, 213, 230, 428, 779
GluI	GCNGC	6 cut(s)	133, 167, 256, 263, 334, 359
HaeII	RGCGCY	1 cut(s)	232
HaeIII	GGCC	3 cut(s)	5, 166, 486
HapII	CCGG	9 cut(s)	114, 150, 215, 266, 286, 326, 483, 507, 667
HgaI	GACGC	3 cut(s)	124, 331, 375
HhaI	GCGC	5 cut(s)	132, 214, 231, 429, 780
Hin1I	GRCGYC	2 cut(s)	237, 323
Hin1II	CATG	4 cut(s)	106, 199, 253, 634
Hin6I	GCGC	5 cut(s)	130, 212, 229, 427, 778
HinP1I	GCGC	5 cut(s)	130, 212, 229, 427, 778
HindIII	AAGCTT	1 cut(s)	635
HinfI	GANTC	4 cut(s)	278, 417, 510, 659
HpaII	CCGG	9 cut(s)	114, 150, 215, 266, 286, 326, 483, 507, 667
HphI	GGTGA	6 cut(s)	32, 166, 283, 463, 485, 541
Hpy166II	GTNNAC	4 cut(s)	241, 442, 616, 718
Hpy188I	TCNGA	3 cut(s)	56, 458, 550
Hpy188III	TCNNGA	2 cut(s)	286, 631
Hpy8I	GTNNAC	4 cut(s)	241, 442, 616, 718
Hpy99I	CGWCG	6 cut(s)	12, 122, 158, 188, 239, 391
HpyAV	CCTTC	2 cut(s)	70, 361
HpyCH4III	ACNGT	1 cut(s)	756
HpyCH4IV	ACGT	2 cut(s)	237, 438
HpyCH4V	TGCA	6 cut(s)	208, 303, 358, 581, 616, 733
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 209, 252, 309, 322, 342, 355, 552, 566
HpyF3I	CTNAG	2 cut(s)	362, 547
HpySE526I	ACGT	2 cut(s)	237, 438
Hsp92I	GRCGYC	2 cut(s)	237, 323
Hsp92II	CATG	4 cut(s)	106, 199, 253, 634
HspAI	GCGC	5 cut(s)	130, 212, 229, 427, 778
Kpn2I	TCCGGA	1 cut(s)	285
KroI	GCCGGC	1 cut(s)	482
KroNI	GCCGGC	1 cut(s)	484
Ksp22I	TGATCA	2 cut(s)	453, 531
Kzo9I	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
LmnI	GCTCC	1 cut(s)	370
Lsp1109I	GCAGC	2 cut(s)	119, 370
LweI	GCATC	1 cut(s)	195
MaeII	ACGT	2 cut(s)	237, 438
MaeIII	GTNAC	4 cut(s)	94, 136, 172, 604
MalI	GATC	6 cut(s)	58, 91, 161, 284, 455, 533
MbiI	CCGCTC	1 cut(s)	169
MboI	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
MboII	GAAGA	2 cut(s)	214, 301
MfeI	CAATTG	2 cut(s)	397, 721
MflI	RGATCY	1 cut(s)	56
MhlI	GDGCHC	4 cut(s)	16, 347, 495, 618
MluCI	AATT	3 cut(s)	397, 721, 781
MluI	ACGCGT	1 cut(s)	384
MlyI	GAGTC	4 cut(s)	272, 411, 519, 668
Mph1103I	ATGCAT	1 cut(s)	583
MroI	TCCGGA	1 cut(s)	285
MroNI	GCCGGC	1 cut(s)	482
MroXI	GAANNNNTTC	1 cut(s)	501
MseI	TTAA	1 cut(s)	318
MslI	CAYNNNNRTG	2 cut(s)	101, 449
MspA1I	CMGCKG	1 cut(s)	361
MspCI	CTTAAG	1 cut(s)	317
MspI	CCGG	9 cut(s)	114, 150, 215, 266, 286, 326, 483, 507, 667
MspR9I	CCNGG	5 cut(s)	114, 150, 414, 507, 668
MunI	CAATTG	2 cut(s)	397, 721
MvaI	CCWGG	1 cut(s)	414
MvnI	CGCG	5 cut(s)	130, 212, 258, 386, 427
MwoI	GCNNNNNNNGC	9 cut(s)	11, 209, 252, 309, 322, 342, 355, 552, 566
NaeI	GCCGGC	1 cut(s)	484
NciI	CCSGG	4 cut(s)	114, 150, 507, 668
NdeII	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
NgoMIV	GCCGGC	1 cut(s)	482
NlaIII	CATG	4 cut(s)	106, 199, 253, 634
NlaIV	GGNNCC	2 cut(s)	58, 124
NmuCI	GTSAC	4 cut(s)	94, 136, 172, 604
NsiI	ATGCAT	1 cut(s)	583
NspI	RCATGY	1 cut(s)	106
PaeR7I	CTCGAG	1 cut(s)	488
PagI	TCATGA	1 cut(s)	630
PcsI	WCGNNNNNNNCGW	4 cut(s)	168, 231, 243, 486
PdiI	GCCGGC	1 cut(s)	484
PdmI	GAANNNNTTC	1 cut(s)	501
PfoI	TCCNGGA	1 cut(s)	505
PkrI	GCNGC	6 cut(s)	134, 168, 257, 264, 335, 360
Ple19I	CGATCG	1 cut(s)	92
PleI	GAGTC	4 cut(s)	272, 411, 518, 667
PpsI	GAGTC	4 cut(s)	272, 411, 518, 667
PshAI	GACNNNNGTC	1 cut(s)	326
Psp124BI	GAGCTC	1 cut(s)	347
Psp6I	CCWGG	1 cut(s)	412
PspGI	CCWGG	1 cut(s)	412
PspN4I	GGNNCC	2 cut(s)	58, 124
PspPI	GGNCC	2 cut(s)	4, 164
PspXI	VCTCGAGB	1 cut(s)	488
PsuI	RGATCY	1 cut(s)	56
PvuI	CGATCG	1 cut(s)	92
PvuII	CAGCTG	1 cut(s)	361
RsaI	GTAC	2 cut(s)	383, 479
RsaNI	GTAC	2 cut(s)	382, 478
RseI	CAYNNNNRTG	2 cut(s)	101, 449
SacI	GAGCTC	1 cut(s)	347
SaqAI	TTAA	1 cut(s)	318
SatI	GCNGC	6 cut(s)	133, 167, 256, 263, 334, 359
Sau3AI	GATC	6 cut(s)	56, 89, 159, 282, 453, 531
Sau96I	GGNCC	2 cut(s)	4, 164
SchI	GAGTC	4 cut(s)	272, 411, 519, 668
ScrFI	CCNGG	5 cut(s)	114, 150, 414, 507, 668
SduI	GDGCHC	4 cut(s)	16, 347, 495, 618
SfaNI	GCATC	1 cut(s)	195
Sfr274I	CTCGAG	1 cut(s)	488
SgrAI	CRCCGGYG	1 cut(s)	214
SlaI	CTCGAG	1 cut(s)	488
SmiMI	CAYNNNNRTG	2 cut(s)	101, 449
SmlI	CTYRAG	4 cut(s)	26, 317, 488, 524
SmoI	CTYRAG	4 cut(s)	26, 317, 488, 524
Sse9I	AATT	3 cut(s)	397, 721, 781
SsiI	CCGC	9 cut(s)	43, 128, 167, 256, 263, 312, 333, 336, 641
SstI	GAGCTC	1 cut(s)	347
StyD4I	CCNGG	5 cut(s)	112, 148, 412, 505, 666
TaaI	ACNGT	1 cut(s)	756
TaiI	ACGT	2 cut(s)	240, 441
TaqI	TCGA	5 cut(s)	10, 186, 225, 281, 489
TaqII	GACCGA	1 cut(s)	82
TasI	AATT	3 cut(s)	397, 721, 781
TauI	GCSGC	4 cut(s)	169, 258, 265, 336
Tru1I	TTAA	1 cut(s)	318
Tru9I	TTAA	1 cut(s)	318
TseFI	GTSAC	4 cut(s)	94, 136, 172, 604
TseI	GCWGC	2 cut(s)	132, 358
Tsp45I	GTSAC	4 cut(s)	94, 136, 172, 604
TspDTI	ATGAA	1 cut(s)	647
TspGWI	ACGGA	1 cut(s)	135
Vha464I	CTTAAG	1 cut(s)	317
VneI	GTGCAC	1 cut(s)	614
XceI	RCATGY	1 cut(s)	106
XhoI	CTCGAG	1 cut(s)	488
XmnI	GAANNNNTTC	1 cut(s)	501
ZraI	GACGTC	1 cut(s)	238
Zsp2I	ATGCAT	1 cut(s)	583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.