Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005225

Physical Location & Seq

Genomic Coordinates Reverse (-)

16774142 .. 16776371

Gene Structure 2230 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G35260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1104 bp

ATGTCTCGCAGATCGCTAACTCTCCTCAAGAATCTAGCTAGAAACGCGAATGGATCAGGTATTCAAACCCGATCCGTGACTTACATGCCCAGACCCGGTGACGGAGCACCACGCGCAGTGACGCTGATCCCCGGAGATGGAATCGGTCCTCTGGTGACGAACGCCGTGGAACAGGTGATGGAAGCGATGCACGCTCCGATCTTCTTCGAGAAGTACGATGTTCACGGAGAAATGAGCCGTGTGCCGCCGGAAGTGATGGAGTCGATAAGGAAGAATAAGGTTTGTTTGAAAGGTGGGCTTAAGACTCCTGTTGGTGGTGGTGTTAGTTCTCTTAACGTCCAGCTTAGGAAAGAGCTTGACCTCTTTGCTTCTCTGGTCAACTGTTTTAATTTGCCTGGTTTACCTACTCGCCATGAGAATGTTGACATCGTTGTCATCAGAGAGAACACTGAAGGTGAGTACGCGGGGCTTGAGCACGAGGTTGTTCCTGGTGTCGTTGAGAGTCTGAAGGTGATCACGAAGTTCTGTTCGGAGCGTATTGCAAAGTATGCTTTTGAGTATGCCTACCTGAACAACAGGAAGAAAGTTACAGCGGTGCATAAGGCTAACATCATGAAACTTGCGGATGGTTTGTTCTTGGAATCTTGTCGCGAGGTTGCGAAAAAGTATCCCAGCATCACCTACAATGAAATCATTGTTGATAACTGCTGTATGCAACTTGTAGCCAAACCAGAGCAATTCGATGTCATGGTAACGCCGAATCTCTATGGGAATTTAGTTGCAAACACTGCTGCTGGTATTGCTGGAGGCACAGGAGTCATGCCTGGAGGAAATGTTGGGGCTGACCATGCGGTATTCGAGCAAGGTGCATCAGCAGGAAATGTTGGGAAAGACAAGATAGTATTGGAAAACAAAGCAAACCCAGTGGCATTGCTTCTCTCATCAGCGATGATGCTTAGACACCTTCAGTTTCCTTCATTTGCTGACCGGCTCGAAACAGCAGTGAAGAAAGTCATCGCTGAAGGAAAGTGCCGGACGAAAGATCTTGGTGGAACAAGCACGACTCAAGAGGTCGTTGATGCGGTCATTGCAAAACTAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006102 GO:0008150 GO:0008152 GO:0008270 GO:0009060 GO:0009987 GO:0015980 GO:0016999 GO:0017144 GO:0019752 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046872 GO:0046914 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00020 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

367

Amino Acids

39.63

Weight (kDa)

8.36

Isoelectric Point (pI)

35.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	40 - 362	1.9e-82	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17130 AT2G17130 AT4G35260
fragaria_vesca	FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520 FvH4_2g16520
malus_domestica	MD05G1133600.v1.1 MD10G1138600.v1.1
prunus_persica	Prupe.3G128300_v2.0.a1 Prupe.8G177400_v2.0.a1 Prupe.8G177400_v2.0.a1 Prupe.8G177400_v2.0.a1
pyrus_communis	pycom07g23620
rosa_chinensis	RchiOBHm_Chr6g0280541
rosa_laevigata	RLG00000013056
rosa_multiflora	Rmu_co8141332.1_g000001 Rmu_sc0005585.1_g000001 Rmu_sc0015818.1_g000005
rosa_roxburghii	Rroxscaffold_7G00188000
rosa_rugosa	Rorug06G0131900 Rorug06G0132000
rosa_samantha	Rh6AG244400 Rh6BG246800 Rh6CG248200 Rh6DG239600
rosa_wichuraiana	Rw6G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	431
AccII	CGCG	4 cut(s)	47, 114, 464, 651
AciI	CCGC	6 cut(s)	245, 464, 593, 623, 851, 1082
AclWI	GGATC	3 cut(s)	61, 66, 121
AcsI	RAATTY	1 cut(s)	772
AcuI	CTGAAG	4 cut(s)	471, 527, 950, 1041
AfaI	GTAC	2 cut(s)	215, 461
AfiI	CCNNNNNNNGG	4 cut(s)	95, 101, 137, 314
AflII	CTTAAG	1 cut(s)	299
AgsI	TTSAA	2 cut(s)	65, 289
AjnI	CCWGG	3 cut(s)	394, 487, 823
AloI	GAACNNNNNNTCC	2 cut(s)	617, 649
AluBI	AGCT	3 cut(s)	38, 343, 355
AluI	AGCT	3 cut(s)	38, 343, 355
Alw21I	GWGCWC	2 cut(s)	109, 477
Alw26I	GTCTC	1 cut(s)	9
AlwI	GGATC	3 cut(s)	61, 66, 121
ApeKI	GCWGC	1 cut(s)	791
ApoI	RAATTY	1 cut(s)	772
AspLEI	GCGC	1 cut(s)	116
AspS9I	GGNCC	1 cut(s)	146
AsuC2I	CCSGG	2 cut(s)	96, 132
AsuHPI	GGTGA	6 cut(s)	110, 166, 187, 467, 523, 670
AvaII	GGWCC	1 cut(s)	146
BauI	CACGAG	1 cut(s)	476
Bbv12I	GWGCWC	2 cut(s)	109, 477
BbvI	GCAGC	1 cut(s)	778
BccI	CCATC	4 cut(s)	131, 172, 250, 620
BceAI	ACGGC	2 cut(s)	149, 222
BcgI	CGANNNNNNTGC	2 cut(s)	848, 882
BciT130I	CCWGG	3 cut(s)	396, 489, 825
BciVI	GTATCC	1 cut(s)	678
BclI	TGATCA	1 cut(s)	513
BcnI	CCSGG	2 cut(s)	96, 132
BcoDI	GTCTC	1 cut(s)	9
BfaI	CTAG	3 cut(s)	35, 39, 1097
BfrI	CTTAAG	1 cut(s)	299
BfuI	GTATCC	1 cut(s)	678
BglII	AGATCT	1 cut(s)	1042
BisI	GCNGC	2 cut(s)	245, 792
BlsI	GCNGC	2 cut(s)	246, 793
Bme1390I	CCNGG	5 cut(s)	96, 132, 396, 489, 825
Bme18I	GGWCC	1 cut(s)	146
BmgT120I	GGNCC	1 cut(s)	146
BmrFI	CCNGG	5 cut(s)	96, 132, 396, 489, 825
BmrI	ACTGGG	1 cut(s)	917
BmsI	GCATC	5 cut(s)	177, 684, 878, 942, 1069
BmuI	ACTGGG	1 cut(s)	917
BpmI	CTGGAG	2 cut(s)	825, 846
Bpu10I	CCTNAGC	1 cut(s)	344
BpuEI	CTTGAG	3 cut(s)	11, 491, 1050
BpuMI	CCSGG	2 cut(s)	96, 132
BsaJI	CCNNGG	2 cut(s)	130, 165
BsaXI	ACNNNNNCTCC	2 cut(s)	819, 849
Bsc4I	CCNNNNNNNGG	4 cut(s)	95, 101, 137, 314
Bse118I	RCCGGY	1 cut(s)	987
Bse1I	ACTGG	1 cut(s)	923
Bse3DI	GCAATG	2 cut(s)	929, 1086
BseBI	CCWGG	3 cut(s)	396, 489, 825
BseDI	CCNNGG	2 cut(s)	130, 165
BseGI	GGATG	1 cut(s)	631
BseLI	CCNNNNNNNGG	4 cut(s)	95, 101, 137, 314
BseMI	GCAATG	2 cut(s)	929, 1086
BseNI	ACTGG	1 cut(s)	923
BseRI	GAGGAG	1 cut(s)	14
BseXI	GCAGC	1 cut(s)	778
BseYI	CCCAGC	1 cut(s)	671
Bsh1236I	CGCG	4 cut(s)	47, 114, 464, 651
BsiHKAI	GWGCWC	2 cut(s)	109, 477
BsiSI	CCGG	5 cut(s)	96, 132, 248, 988, 1033
BslI	CCNNNNNNNGG	4 cut(s)	95, 101, 137, 314
BsmAI	GTCTC	1 cut(s)	9
Bsp1286I	GDGCHC	2 cut(s)	109, 477
Bsp143I	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
Bsp68I	TCGCGA	1 cut(s)	651
BspACI	CCGC	6 cut(s)	245, 464, 593, 623, 851, 1082
BspFNI	CGCG	4 cut(s)	47, 114, 464, 651
BspHI	TCATGA	1 cut(s)	612
BspPI	GGATC	3 cut(s)	61, 66, 121
BspTI	CTTAAG	1 cut(s)	299
BsrDI	GCAATG	2 cut(s)	929, 1086
BsrFI	RCCGGY	1 cut(s)	987
BsrI	ACTGG	1 cut(s)	923
BssAI	RCCGGY	1 cut(s)	987
BssECI	CCNNGG	2 cut(s)	130, 165
BssMI	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
BssSI	CACGAG	1 cut(s)	476
Bst2BI	CACGAG	1 cut(s)	476
Bst2UI	CCWGG	3 cut(s)	396, 489, 825
Bst4CI	ACNGT	1 cut(s)	383
BstAFI	CTTAAG	1 cut(s)	299
BstAPI	GCANNNNNTGC	2 cut(s)	548, 788
BstC8I	GCNNGC	1 cut(s)	192
BstDEI	CTNAG	2 cut(s)	344, 956
BstDSI	CCRYGG	1 cut(s)	165
BstF5I	GGATG	1 cut(s)	631
BstFNI	CGCG	4 cut(s)	47, 114, 464, 651
BstHHI	GCGC	1 cut(s)	116
BstKTI	GATC	7 cut(s)	14, 56, 74, 129, 201, 516, 1045
BstMAI	GTCTC	1 cut(s)	9
BstMBI	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 113, 191, 548, 788, 800, 848, 1088
BstNI	CCWGG	3 cut(s)	396, 489, 825
BstNSI	RCATGY	1 cut(s)	88
BstSCI	CCNGG	5 cut(s)	94, 130, 394, 487, 823
BstUI	CGCG	4 cut(s)	47, 114, 464, 651
BstV1I	GCAGC	1 cut(s)	778
BstX2I	RGATCY	1 cut(s)	1042
BstYI	RGATCY	1 cut(s)	1042
BsuI	GTATCC	1 cut(s)	678
BtgI	CCRYGG	1 cut(s)	165
BtgZI	GCGATG	3 cut(s)	200, 962, 1000
BtsCI	GGATG	1 cut(s)	631
BtsI	GCAGTG	3 cut(s)	123, 786, 1008
BtsIMutI	CAGTG	5 cut(s)	123, 447, 786, 930, 1008
BtuMI	TCGCGA	1 cut(s)	651
Cac8I	GCNNGC	1 cut(s)	192
CciI	TCATGA	1 cut(s)	612
CfoI	GCGC	1 cut(s)	116
Cfr10I	RCCGGY	1 cut(s)	987
Cfr13I	GGNCC	1 cut(s)	146
CseI	GACGC	1 cut(s)	130
Csp6I	GTAC	2 cut(s)	214, 460
CspCI	CAANNNNNGTGG	2 cut(s)	906, 941
CviAII	CATG	6 cut(s)	85, 413, 613, 748, 820, 848
CviQI	GTAC	2 cut(s)	214, 460
DdeI	CTNAG	2 cut(s)	344, 956
DpnI	GATC	7 cut(s)	13, 55, 73, 128, 200, 515, 1044
DpnII	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
DrdI	GACNNNNNNGTC	1 cut(s)	431
DseDI	GACNNNNNNGTC	1 cut(s)	431
Eco47I	GGWCC	1 cut(s)	146
Eco57I	CTGAAG	4 cut(s)	471, 527, 950, 1041
EcoRII	CCWGG	3 cut(s)	394, 487, 823
FaeI	CATG	6 cut(s)	88, 416, 616, 751, 823, 851
FalI	AAGNNNNNCTT	2 cut(s)	282, 314
FatI	CATG	6 cut(s)	84, 412, 612, 747, 819, 847
FauI	CCCGC	1 cut(s)	457
FbaI	TGATCA	1 cut(s)	513
Fnu4HI	GCNGC	2 cut(s)	245, 792
FokI	GGATG	1 cut(s)	638
Fsp4HI	GCNGC	2 cut(s)	245, 792
FspBI	CTAG	3 cut(s)	35, 39, 1097
GlaI	GCGC	1 cut(s)	115
GluI	GCNGC	2 cut(s)	245, 792
GsaI	CCCAGC	1 cut(s)	675
GsuI	CTGGAG	2 cut(s)	825, 846
HapII	CCGG	5 cut(s)	96, 132, 248, 988, 1033
HgaI	GACGC	1 cut(s)	130
HhaI	GCGC	1 cut(s)	116
Hin1II	CATG	6 cut(s)	88, 416, 616, 751, 823, 851
Hin6I	GCGC	1 cut(s)	114
HinP1I	GCGC	1 cut(s)	114
HincII	GTYRAC	2 cut(s)	379, 424
HindII	GTYRAC	2 cut(s)	379, 424
HinfI	GANTC	9 cut(s)	31, 141, 260, 304, 502, 641, 760, 816, 1063
HpaII	CCGG	5 cut(s)	96, 132, 248, 988, 1033
HphI	GGTGA	6 cut(s)	110, 166, 187, 467, 523, 670
Hpy166II	GTNNAC	4 cut(s)	223, 379, 401, 424
Hpy188I	TCNGA	4 cut(s)	198, 440, 507, 532
Hpy188III	TCNNGA	6 cut(s)	28, 208, 517, 613, 650, 1067
Hpy8I	GTNNAC	4 cut(s)	223, 379, 401, 424
HpyAV	CCTTC	5 cut(s)	446, 502, 974, 984, 1016
HpyCH4III	ACNGT	1 cut(s)	383
HpyCH4IV	ACGT	1 cut(s)	336
HpyCH4V	TGCA	7 cut(s)	190, 542, 598, 715, 782, 869, 1091
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 113, 191, 548, 788, 800, 848, 1088
HpyF3I	CTNAG	2 cut(s)	344, 956
HpySE526I	ACGT	1 cut(s)	336
Hsp92II	CATG	6 cut(s)	88, 416, 616, 751, 823, 851
HspAI	GCGC	1 cut(s)	114
Ksp22I	TGATCA	1 cut(s)	513
Kzo9I	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
LmnI	GCTCC	3 cut(s)	104, 199, 532
Lsp1109I	GCAGC	1 cut(s)	778
LweI	GCATC	5 cut(s)	177, 684, 878, 942, 1069
MaeI	CTAG	3 cut(s)	35, 39, 1097
MaeII	ACGT	1 cut(s)	336
MaeIII	GTNAC	6 cut(s)	76, 98, 118, 154, 586, 751
MalI	GATC	7 cut(s)	13, 55, 73, 128, 200, 515, 1044
MboI	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
MboII	GAAGA	5 cut(s)	193, 196, 283, 592, 1018
MflI	RGATCY	1 cut(s)	1042
MhlI	GDGCHC	2 cut(s)	109, 477
MluCI	AATT	3 cut(s)	388, 737, 772
MlyI	GAGTC	5 cut(s)	269, 298, 511, 825, 1057
MnlI	CCTC	8 cut(s)	35, 159, 371, 472, 646, 800, 821, 1063
MseI	TTAA	3 cut(s)	300, 333, 387
MslI	CAYNNNNRTG	1 cut(s)	417
MspA1I	CMGCKG	1 cut(s)	593
MspCI	CTTAAG	1 cut(s)	299
MspI	CCGG	5 cut(s)	96, 132, 248, 988, 1033
MspR9I	CCNGG	5 cut(s)	96, 132, 396, 489, 825
MvaI	CCWGG	3 cut(s)	396, 489, 825
MvnI	CGCG	4 cut(s)	47, 114, 464, 651
MwoI	GCNNNNNNNGC	8 cut(s)	44, 113, 191, 548, 788, 800, 848, 1088
NciI	CCSGG	2 cut(s)	96, 132
NdeII	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
NlaIII	CATG	6 cut(s)	88, 416, 616, 751, 823, 851
NmuCI	GTSAC	4 cut(s)	76, 98, 118, 154
NruI	TCGCGA	1 cut(s)	651
NspI	RCATGY	1 cut(s)	88
PagI	TCATGA	1 cut(s)	612
PcsI	WCGNNNNNNNCGW	1 cut(s)	213
PfeI	GAWTC	4 cut(s)	31, 141, 641, 760
PkrI	GCNGC	2 cut(s)	246, 793
PleI	GAGTC	5 cut(s)	268, 298, 510, 824, 1057
PpsI	GAGTC	5 cut(s)	268, 298, 510, 824, 1057
Psp6I	CCWGG	3 cut(s)	394, 487, 823
PspFI	CCCAGC	1 cut(s)	671
PspGI	CCWGG	3 cut(s)	394, 487, 823
PspPI	GGNCC	1 cut(s)	146
PsuI	RGATCY	1 cut(s)	1042
RruI	TCGCGA	1 cut(s)	651
RsaI	GTAC	2 cut(s)	215, 461
RsaNI	GTAC	2 cut(s)	214, 460
RseI	CAYNNNNRTG	1 cut(s)	417
SaqAI	TTAA	3 cut(s)	300, 333, 387
SatI	GCNGC	2 cut(s)	245, 792
Sau3AI	GATC	7 cut(s)	11, 53, 71, 126, 198, 513, 1042
Sau96I	GGNCC	1 cut(s)	146
SchI	GAGTC	5 cut(s)	269, 298, 511, 825, 1057
ScrFI	CCNGG	5 cut(s)	96, 132, 396, 489, 825
SduI	GDGCHC	2 cut(s)	109, 477
SfaNI	GCATC	5 cut(s)	177, 684, 878, 942, 1069
SinI	GGWCC	1 cut(s)	146
SmiMI	CAYNNNNRTG	1 cut(s)	417
SmlI	CTYRAG	4 cut(s)	26, 299, 470, 1065
SmoI	CTYRAG	4 cut(s)	26, 299, 470, 1065
Sse9I	AATT	3 cut(s)	388, 737, 772
SsiI	CCGC	6 cut(s)	245, 464, 593, 623, 851, 1082
SspMI	CTAG	3 cut(s)	35, 39, 1097
StyD4I	CCNGG	5 cut(s)	94, 130, 394, 487, 823
TaaI	ACNGT	1 cut(s)	383
TaiI	ACGT	1 cut(s)	339
TaqI	TCGA	5 cut(s)	207, 263, 741, 858, 993
TaqII	GACCGA	1 cut(s)	134
TasI	AATT	3 cut(s)	388, 737, 772
TauI	GCSGC	1 cut(s)	247
TfiI	GAWTC	4 cut(s)	31, 141, 641, 760
Tru1I	TTAA	3 cut(s)	300, 333, 387
Tru9I	TTAA	3 cut(s)	300, 333, 387
TscAI	CASTG	5 cut(s)	123, 454, 793, 930, 1008
TseFI	GTSAC	4 cut(s)	76, 98, 118, 154
TseI	GCWGC	1 cut(s)	791
Tsp45I	GTSAC	4 cut(s)	76, 98, 118, 154
TspDTI	ATGAA	3 cut(s)	629, 702, 966
TspGWI	ACGGA	3 cut(s)	64, 117, 240
TspRI	CASTG	5 cut(s)	123, 454, 793, 930, 1008
Vha464I	CTTAAG	1 cut(s)	299
VpaK11BI	GGWCC	1 cut(s)	146
XapI	RAATTY	1 cut(s)	772
XceI	RCATGY	1 cut(s)	88
XspI	CTAG	3 cut(s)	35, 39, 1097

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.