AT2G18080

serine protease EDA2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004990
Physical Location & Seq
Forward (+)
7857139 .. 7859983
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G18080.1

Sequence Viewer

Length: 1470 bp
ATGTCGTTAGAGTTCGGCTTTATTCTGATCAACATTTTCACTGCGATTGTGTCATTTTCAACTTTGTCTCATGCCTTATTGCATCCTAGTAGCGTTTCTCATAATGTCTCCAGAAGCCGCTATTACATGACGACCAATGAACTATGGTTTAATCAAACCCTAGATCATGAATCTCCTAATGATCACCGCAAATTCAGACAGCGATACTATGAATTTATGGACTACTTTCGATCTCCAGATGGGCCTATGTTTATGATAATATGCGGTGAAGGTCCTTGTAGTGGCATAGCTAATGATTATATAAATGTATTAGCAAAGAAGTTTCAAGCAGGTGTGGTTTCACTAGAACATAGGTACTATGGAAAAAGCTCCCCCTTTAACTCATTAGCTACAGAAAATTTGAAATACCTTTCATCGAAACAAGCCCTTTACGATTTGGCATCTTTCCGACAATATTACCAGGAATCTCTGAATAAGAAGTTGAATATAAGTAGTGGTGGTAGTGACAATCCGTGGTTCTTCTTCGGGATTTCTTATTCTGGAGCTTTAAGTGCGTGGTTTCGGCTTAAGTTCCCGCATTTGACGTGTGGAAGCCTTGCTAGTTCTGCAGTCGTTCGTGCAATCTATGAATTCTCTGAATTTGATCAGCAGATAGGTGAATCAGCTGGTCAAGAATGCAAACTTGCACTACAAGAGACTAATAAACTTCTGGAATTAGGGCTTAAAGTAAAAAATAAGGCAGTGAAATCTCTCTTCAATGCCACTGAGCTTGATGTTGATGCTGATTTTTTATACTTAACGGCAGATGCAGCGGTTATGGCGTTTCAATATGGAAATCCAGATAAACTATGTGTCCCGCTTGTTGAAGCAAAGAAGAATGGTAGTGATTTAGTGGTAACATATTCAACATATGTTAGAGAGTATTGCATGAGAATTTGGGGATTACGTGTTAGAACATATAACCGAAAACATCTGAGAAACACAGTAGTTACCGCAGACAGTGCATATCGGTTATGGTGGTTTCAAGCTTGCACAGAACTAGGATATTTCCAGGTGGCACCTAAATATGATAGTGTTCGATCTCATCAAATCAATACAACGTTTCATTTGGATTTATGCAAGAGCCTTTTTGGGAAAGATGTTTACCCTAAAGTTGATGCAACAAACTTGTACTATGGAGGCGATAGACTCGCCGCAACCAAAATCATTTTCACAAATGGGTCAGAAGATCCATGGCGTCATGCTTCCAAACAGAACTCTACTCATGAAATGCCTTCTTACATCATAAAATGTCGTAACTGTGGCCATGGAAGTGATATAAGAGGATGCCCTCAATCTCCAATGGTCATTGAAGGTAAATCAAATAATTGCAGCTTACCCGATTATGTAAACAAAGTAAGGCAACAGATGGTAGAACACATTGACTTGTGGCTGTCTGAGTGTCGCCAATCTATTAGGAGTTCAATATAG

Protein Analysis

489

Amino Acids

55.81

Weight (kDa)

8.52

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 52 - 441 6.7e-73 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 1057
AciI CCGC 8 cut(s) 118, 187, 264, 575, 812, 857, 993, 1194
AclI AACGTT 1 cut(s) 1100
AclWI GGATC 1 cut(s) 1223
AcoI YGGCCR 1 cut(s) 1303
AcsI RAATTY 6 cut(s) 191, 212, 397, 629, 638, 933
AcyI GRCGYC 1 cut(s) 1237
AfaI GTAC 2 cut(s) 356, 1172
AfiI CCNNNNNNNGG 1 cut(s) 281
AflII CTTAAG 1 cut(s) 566
AflIII ACRYGT 2 cut(s) 584, 946
AjiI CACGTC 1 cut(s) 585
AjnI CCWGG 2 cut(s) 459, 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AluBI AGCT 8 cut(s) 290, 369, 389, 545, 665, 769, 1028, 1374
AluI AGCT 8 cut(s) 290, 369, 389, 545, 665, 769, 1028, 1374
Alw26I GTCTC 3 cut(s) 72, 112, 689
AlwI GGATC 1 cut(s) 1223
AoxI GGCC 2 cut(s) 242, 1303
ApeKI GCWGC 2 cut(s) 809, 1371
ApoI RAATTY 6 cut(s) 191, 212, 397, 629, 638, 933
AspS9I GGNCC 2 cut(s) 242, 272
AsuHPI GGTGA 3 cut(s) 176, 278, 668
AvaII GGWCC 1 cut(s) 272
BalI TGGCCA 1 cut(s) 1305
BanI GGYRCC 1 cut(s) 1057
BbvI GCAGC 2 cut(s) 821, 1383
BccI CCATC 2 cut(s) 233, 1402
BceAI ACGGC 1 cut(s) 816
BciT130I CCWGG 2 cut(s) 461, 1052
BclI TGATCA 3 cut(s) 27, 181, 643
BcoDI GTCTC 3 cut(s) 72, 112, 689
BfaI CTAG 5 cut(s) 87, 161, 344, 600, 1040
BfmI CTRYAG 2 cut(s) 390, 606
BfrI CTTAAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 320
BisI GCNGC 4 cut(s) 118, 810, 1194, 1372
BlsI GCNGC 4 cut(s) 119, 811, 1195, 1373
Bme1390I CCNGG 2 cut(s) 461, 1052
Bme18I GGWCC 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 242, 272
BmiI GGNNCC 1 cut(s) 1059
BmrFI CCNGG 2 cut(s) 461, 1052
BmsI GCATC 6 cut(s) 91, 449, 769, 796, 1147, 1316
BpmI CTGGAG 3 cut(s) 94, 219, 561
BsaAI YACGTR 1 cut(s) 947
BsaHI GRCGYC 1 cut(s) 1237
BsaJI CCNNGG 3 cut(s) 512, 1232, 1306
Bsc4I CCNNNNNNNGG 1 cut(s) 281
BseBI CCWGG 2 cut(s) 461, 1052
BseDI CCNNGG 3 cut(s) 512, 1232, 1306
BseGI GGATG 2 cut(s) 82, 1331
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMII CTCAG 3 cut(s) 756, 965, 1428
BseXI GCAGC 2 cut(s) 821, 1383
BshFI GGCC 2 cut(s) 244, 1305
BshNI GGYRCC 1 cut(s) 1057
BslFI GGGAC 1 cut(s) 839
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 3 cut(s) 72, 112, 689
BsmFI GGGAC 1 cut(s) 839
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 2 cut(s) 244, 1305
Bsp143I GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
Bsp19I CCATGG 2 cut(s) 1232, 1306
BspACI CCGC 8 cut(s) 118, 187, 264, 575, 812, 857, 993, 1194
BspANI GGCC 2 cut(s) 244, 1305
BspCNI CTCAG 3 cut(s) 757, 966, 1429
BspHI TCATGA 2 cut(s) 166, 1264
BspLI GGNNCC 1 cut(s) 1059
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 1223
BspT107I GGYRCC 1 cut(s) 1057
BspTI CTTAAG 1 cut(s) 566
BssECI CCNNGG 3 cut(s) 512, 1232, 1306
BssMI GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
BssNI GRCGYC 1 cut(s) 1237
BssT1I CCWWGG 2 cut(s) 1232, 1306
Bst2UI CCWGG 2 cut(s) 461, 1052
Bst4CI ACNGT 3 cut(s) 985, 1001, 1301
Bst6I CTCTTC 1 cut(s) 758
BstACI GRCGYC 1 cut(s) 1237
BstAFI CTTAAG 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstBAI YACGTR 1 cut(s) 947
BstC8I GCNNGC 1 cut(s) 1030
BstDEI CTNAG 3 cut(s) 765, 974, 1437
BstDSI CCRYGG 3 cut(s) 512, 1232, 1306
BstF5I GGATG 2 cut(s) 82, 1331
BstKTI GATC 7 cut(s) 30, 166, 184, 233, 646, 1082, 1231
BstMAI GTCTC 3 cut(s) 72, 112, 689
BstMBI GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
BstMWI GCNNNNNNNGC 5 cut(s) 551, 605, 809, 818, 1001
BstNI CCWGG 2 cut(s) 461, 1052
BstSCI CCNGG 2 cut(s) 459, 1050
BstSFI CTRYAG 2 cut(s) 390, 606
BstV1I GCAGC 2 cut(s) 821, 1383
BstX2I RGATCY 1 cut(s) 1228
BstYI RGATCY 1 cut(s) 1228
BsuRI GGCC 2 cut(s) 244, 1305
BtgI CCRYGG 3 cut(s) 512, 1232, 1306
BtrI CACGTC 1 cut(s) 585
BtsCI GGATG 2 cut(s) 82, 1331
BtsI GCAGTG 2 cut(s) 39, 747
BtsIMutI CAGTG 4 cut(s) 39, 747, 762, 1006
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 1 cut(s) 1030
CciI TCATGA 2 cut(s) 166, 1264
Cfr13I GGNCC 2 cut(s) 242, 272
CseI GACGC 1 cut(s) 1226
Csp6I GTAC 2 cut(s) 355, 1171
CspCI CAANNNNNGTGG 2 cut(s) 751, 786
CviAII CATG 8 cut(s) 71, 127, 167, 928, 1233, 1241, 1265, 1307
CviQI GTAC 2 cut(s) 355, 1171
DdeI CTNAG 3 cut(s) 765, 974, 1437
DpnI GATC 7 cut(s) 29, 165, 183, 232, 645, 1081, 1230
DpnII GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
EaeI YGGCCR 1 cut(s) 1303
Eam1104I CTCTTC 1 cut(s) 758
EarI CTCTTC 1 cut(s) 758
Eco130I CCWWGG 2 cut(s) 1232, 1306
Eco47I GGWCC 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 2 cut(s) 459, 1050
EcoT14I CCWWGG 2 cut(s) 1232, 1306
ErhI CCWWGG 2 cut(s) 1232, 1306
FaeI CATG 8 cut(s) 74, 130, 170, 931, 1236, 1244, 1268, 1310
FaqI GGGAC 1 cut(s) 839
FatI CATG 8 cut(s) 70, 126, 166, 927, 1232, 1240, 1264, 1306
FauI CCCGC 2 cut(s) 582, 864
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 3 cut(s) 27, 181, 643
Fnu4HI GCNGC 4 cut(s) 118, 810, 1194, 1372
FokI GGATG 2 cut(s) 69, 1338
Fsp4HI GCNGC 4 cut(s) 118, 810, 1194, 1372
FspBI CTAG 5 cut(s) 87, 161, 344, 600, 1040
GluI GCNGC 4 cut(s) 118, 810, 1194, 1372
GsuI CTGGAG 3 cut(s) 94, 219, 561
HaeIII GGCC 2 cut(s) 244, 1305
HgaI GACGC 1 cut(s) 1226
Hin1I GRCGYC 1 cut(s) 1237
Hin1II CATG 8 cut(s) 74, 130, 170, 931, 1236, 1244, 1268, 1310
HindIII AAGCTT 1 cut(s) 1026
HinfI GANTC 4 cut(s) 170, 464, 659, 1188
HphI GGTGA 3 cut(s) 176, 278, 668
Hpy166II GTNNAC 2 cut(s) 1144, 1390
Hpy188I TCNGA 8 cut(s) 27, 197, 449, 471, 637, 975, 1225, 1438
Hpy188III TCNNGA 9 cut(s) 111, 167, 236, 526, 540, 671, 710, 839, 1265
Hpy8I GTNNAC 2 cut(s) 1144, 1390
HpyAV CCTTC 3 cut(s) 263, 1284, 1346
HpyCH4III ACNGT 3 cut(s) 985, 1001, 1301
HpyCH4IV ACGT 3 cut(s) 584, 946, 1100
HpyF10VI GCNNNNNNNGC 5 cut(s) 551, 605, 809, 818, 1001
HpyF3I CTNAG 3 cut(s) 765, 974, 1437
HpySE526I ACGT 3 cut(s) 584, 946, 1100
Hsp92I GRCGYC 1 cut(s) 1237
Hsp92II CATG 8 cut(s) 74, 130, 170, 931, 1236, 1244, 1268, 1310
Ksp22I TGATCA 3 cut(s) 27, 181, 643
Kzo9I GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
LmnI GCTCC 2 cut(s) 374, 542
Lsp1109I GCAGC 2 cut(s) 821, 1383
LweI GCATC 6 cut(s) 91, 449, 769, 796, 1147, 1316
MaeI CTAG 5 cut(s) 87, 161, 344, 600, 1040
MaeII ACGT 3 cut(s) 584, 946, 1100
MaeIII GTNAC 4 cut(s) 503, 895, 988, 1295
MalI GATC 7 cut(s) 29, 165, 183, 232, 645, 1081, 1230
MboI GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
MboII GAAGA 5 cut(s) 511, 514, 745, 886, 1238
MflI RGATCY 1 cut(s) 1228
MlsI TGGCCA 1 cut(s) 1305
MluCI AATT 8 cut(s) 191, 212, 397, 629, 638, 713, 933, 1366
MluNI TGGCCA 1 cut(s) 1305
MlyI GAGTC 1 cut(s) 1182
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 3 cut(s) 1172, 1316, 1341
Mox20I TGGCCA 1 cut(s) 1305
MscI TGGCCA 1 cut(s) 1305
MseI TTAA 6 cut(s) 150, 378, 548, 567, 723, 797
MslI CAYNNNNRTG 1 cut(s) 1311
Msp20I TGGCCA 1 cut(s) 1305
MspA1I CMGCKG 2 cut(s) 665, 812
MspCI CTTAAG 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 461, 1052
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 2 cut(s) 461, 1052
MwoI GCNNNNNNNGC 5 cut(s) 551, 605, 809, 818, 1001
NcoI CCATGG 2 cut(s) 1232, 1306
NdeI CATATG 1 cut(s) 910
NdeII GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
NlaIII CATG 8 cut(s) 74, 130, 170, 931, 1236, 1244, 1268, 1310
NlaIV GGNNCC 1 cut(s) 1059
NmuCI GTSAC 1 cut(s) 503
PagI TCATGA 2 cut(s) 166, 1264
PaqCI CACCTGC 1 cut(s) 320
PctI GAATGC 1 cut(s) 680
PfeI GAWTC 3 cut(s) 170, 464, 659
PkrI GCNGC 4 cut(s) 119, 811, 1195, 1373
PleI GAGTC 1 cut(s) 1182
PpsI GAGTC 1 cut(s) 1182
Ppu21I YACGTR 1 cut(s) 947
PpuMI RGGWCCY 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 1100
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 459, 1050
PspGI CCWGG 2 cut(s) 459, 1050
PspN4I GGNNCC 1 cut(s) 1059
PspPI GGNCC 2 cut(s) 242, 272
PspPPI RGGWCCY 1 cut(s) 272
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 1228
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 2 cut(s) 356, 1172
RsaNI GTAC 2 cut(s) 355, 1171
RseI CAYNNNNRTG 1 cut(s) 1311
SaqAI TTAA 6 cut(s) 150, 378, 548, 567, 723, 797
SatI GCNGC 4 cut(s) 118, 810, 1194, 1372
Sau3AI GATC 7 cut(s) 27, 163, 181, 230, 643, 1079, 1228
Sau96I GGNCC 2 cut(s) 242, 272
SchI GAGTC 1 cut(s) 1182
ScrFI CCNGG 2 cut(s) 461, 1052
SfaNI GCATC 6 cut(s) 91, 449, 769, 796, 1147, 1316
SfcI CTRYAG 2 cut(s) 390, 606
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 1311
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 8 cut(s) 191, 212, 397, 629, 638, 713, 933, 1366
SsiI CCGC 8 cut(s) 118, 187, 264, 575, 812, 857, 993, 1194
SspI AATATT 1 cut(s) 455
SspMI CTAG 5 cut(s) 87, 161, 344, 600, 1040
StyD4I CCNGG 2 cut(s) 459, 1050
StyI CCWWGG 2 cut(s) 1232, 1306
TaaI ACNGT 3 cut(s) 985, 1001, 1301
TaiI ACGT 3 cut(s) 587, 949, 1103
TaqI TCGA 3 cut(s) 229, 416, 1078
TasI AATT 8 cut(s) 191, 212, 397, 629, 638, 713, 933, 1366
TatI WGTACW 1 cut(s) 1170
TauI GCSGC 2 cut(s) 120, 1196
TfiI GAWTC 3 cut(s) 170, 464, 659
Tru1I TTAA 6 cut(s) 150, 378, 548, 567, 723, 797
Tru9I TTAA 6 cut(s) 150, 378, 548, 567, 723, 797
TscAI CASTG 4 cut(s) 46, 747, 769, 1006
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 2 cut(s) 809, 1371
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 7 cut(s) 153, 183, 225, 402, 642, 1094, 1281
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 4 cut(s) 46, 747, 769, 1006
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 6 cut(s) 191, 212, 397, 629, 638, 933
XspI CTAG 5 cut(s) 87, 161, 344, 600, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.