MD15G1248500.v1.1

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004990
Physical Location & Seq
Forward (+)
20707826 .. 20709981
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1248500.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGCCGACGTCGTCTTCGTTCTCGCTCTTTCTAACGACGATGCTCTGGACGCTTGCTTCTGCCTTTATGAATCCCCGAACTTTTCTTCAGCTGGGGAGCTCGTCCGGAGCCAGAGGCACTGGCAATTACTTGACGACGGAGGAGTTCTGGTTCGATCAGACTCTCGACCACTTCTCGCCCTACGACAGCCGCAAATTCCCGCAGCGCTACTACGAATTTCTCGACTACTTCAGAACGCCTGACGGACCCATTTTTCTCAGAATTTGCGGAGAAGCCGCGTGCAGCGGCATTGCTAACGATTACCTTAGTGTGTTGGCTAAGAAGTTTGGGGCAGCTGTGGTTTCGCCTGAGCATCGATACTACGGAAAAAGCTCTCCTTTCAAATCGCATACAACGGAGAATTTGAGGTACTTGTCATCCAAGCAAGCTCTTTTCGATTTGGCGATTTTTCGTGAGTTTTACCAGGCAAGCCCCATTTGTGAAGTTTATGAATTTGTTAATTCATTAAATGTGAAGCTGAATAAGACCAAGGGTGAAAATCCATGGTTTGTGTTTGGTGTTTCGTATGCGGGAGCTCTTAGTGCGTGGTTTCGGGTTAAATTCCCGCATTTGACATGTGGAAGTGTTGCAAGTTCTGCTGTTGTTGGAGCTGTTTATAACTTCACTGAATTTGACCAGCAGGTTGGTGAGTCTGCAGGTCCACAATGTAAAGCTGCACTGCAAGAAACTAATCGTCTTGTTGAACAAAGGCTTGCTACTAACCGGAAAGCAGTAAAGGCGTCGTTTGGTGCAACGCAGCTTGATATTGATGATGATTTTATGTATTTTCTGGCCGATGCTGCTGTTACAGCGTTTCAATCCGGAATTCCGGATAAGTTATGCTCCCCTCTTGTTCAAGCAAAGACAAATGAAGAGGATTTGGTGGAAACATATGCTAGATACGTTAAAGATCATTATCTGGGAAGTTTTGGTGTTGATATCGAAACATATAATCAGAAGCACTTGAAGAATACTGCTATTAGTGAGGGCAGTTCTGATAGATTGTGGTGGTTCCAAGTTTGTACTGAAGTTGCATATTTTCAGGTGGCACCTGCAAATGATAGCATCCGGTCTTCAAAAATTGACACAAGGCATGTTTCTGAAAATATTTTTGATCTAAATACTATTTTTCTACTTATGCTTTTTCTACAGACCTGTAGAATGTTCAACTCGGTAGCTCCTGTACGCATCTCTGATTGA

Protein Analysis

413

Amino Acids

46.46

Weight (kDa)

5.87

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 53 - 373 1.1e-55 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AarI CACCTGC 1 cut(s) 1099
AatII GACGTC 1 cut(s) 11
Acc36I ACCTGC 3 cut(s) 670, 686, 1099
AccB1I GGYRCC 1 cut(s) 1087
AccII CGCG 1 cut(s) 278
AccIII TCCGGA 3 cut(s) 104, 860, 868
AciI CCGC 7 cut(s) 190, 200, 267, 276, 285, 569, 605
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 8 cut(s) 194, 215, 261, 400, 491, 599, 668, 864
AcuI CTGAAG 3 cut(s) 71, 214, 1086
AcyI GRCGYC 2 cut(s) 8, 779
AfaI GTAC 3 cut(s) 410, 1063, 1224
AfeI AGCGCT 1 cut(s) 206
AflIII ACRYGT 1 cut(s) 614
AgsI TTSAA 7 cut(s) 382, 743, 857, 896, 1006, 1116, 1207
AjnI CCWGG 1 cut(s) 462
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
Alw21I GWGCWC 2 cut(s) 101, 577
Aor13HI TCCGGA 3 cut(s) 104, 860, 868
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 1 cut(s) 831
ApeKI GCWGC 6 cut(s) 202, 282, 332, 713, 796, 839
ApoI RAATTY 8 cut(s) 194, 215, 261, 400, 491, 599, 668, 864
ArsI GACNNNNNNTTYG 1 cut(s) 30
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 245, 698
AsuHPI GGTGA 2 cut(s) 545, 698
AvaII GGWCC 2 cut(s) 245, 698
BanI GGYRCC 1 cut(s) 1087
BanII GRGCYC 2 cut(s) 101, 577
BbsI GAAGAC 2 cut(s) 6, 1104
Bbv12I GWGCWC 2 cut(s) 101, 577
BbvI GCAGC 6 cut(s) 214, 294, 344, 700, 808, 826
BciT130I CCWGG 1 cut(s) 464
BfaI CTAG 1 cut(s) 936
BfmI CTRYAG 3 cut(s) 693, 1187, 1195
BfoI RGCGCY 1 cut(s) 208
BfuAI ACCTGC 3 cut(s) 670, 686, 1099
BisI GCNGC 9 cut(s) 190, 203, 276, 283, 286, 333, 714, 797, 840
BlsI GCNGC 9 cut(s) 191, 204, 277, 284, 287, 334, 715, 798, 841
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 2 cut(s) 245, 698
BmgT120I GGNCC 2 cut(s) 245, 698
BmiI GGNNCC 4 cut(s) 109, 247, 1052, 1089
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 4 cut(s) 30, 361, 826, 1113
BpiI GAAGAC 2 cut(s) 6, 1104
Bpu10I CCTNAGC 1 cut(s) 348
Bsa29I ATCGAT 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 954
BsaHI GRCGYC 2 cut(s) 8, 779
BsaJI CCNNGG 2 cut(s) 528, 542
BsaWI WCCGGW 5 cut(s) 104, 762, 860, 868, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse1I ACTGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 288
Bse8I GATNNNNATC 1 cut(s) 954
BseAI TCCGGA 3 cut(s) 104, 860, 868
BseBI CCWGG 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 528, 542
BseGI GGATG 2 cut(s) 416, 1104
BseJI GATNNNNATC 1 cut(s) 954
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 2 cut(s) 271, 339
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 6 cut(s) 214, 294, 344, 700, 808, 826
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 2 cut(s) 301, 699
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 1 cut(s) 833
BshNI GGYRCC 1 cut(s) 1087
BshVI ATCGAT 1 cut(s) 355
BsiHKAI GWGCWC 2 cut(s) 101, 577
BsiSI CCGG 5 cut(s) 105, 763, 861, 869, 1108
BsnI GGCC 1 cut(s) 833
Bsp1286I GDGCHC 2 cut(s) 101, 577
Bsp13I TCCGGA 3 cut(s) 104, 860, 868
Bsp143I GATC 3 cut(s) 154, 949, 1153
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 7 cut(s) 190, 200, 267, 276, 285, 569, 605
BspANI GGCC 1 cut(s) 833
BspCNI CTCAG 2 cut(s) 270, 340
BspDI ATCGAT 1 cut(s) 355
BspEI TCCGGA 3 cut(s) 104, 860, 868
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 4 cut(s) 109, 247, 1052, 1089
BspMAI CTGCAG 1 cut(s) 697
BspMI ACCTGC 3 cut(s) 670, 686, 1099
BspT107I GGYRCC 1 cut(s) 1087
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 2 cut(s) 528, 542
BssMI GATC 3 cut(s) 154, 949, 1153
BssNI GRCGYC 2 cut(s) 8, 779
BssT1I CCWWGG 2 cut(s) 528, 542
Bst2UI CCWGG 1 cut(s) 464
Bst6I CTCTTC 1 cut(s) 906
BstACI GRCGYC 2 cut(s) 8, 779
BstAPI GCANNNNNTGC 1 cut(s) 635
BstC8I GCNNGC 5 cut(s) 54, 280, 426, 469, 753
BstDEI CTNAG 5 cut(s) 257, 305, 318, 348, 578
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 2 cut(s) 416, 1104
BstFNI CGCG 1 cut(s) 278
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 157, 952, 1156
BstMBI GATC 3 cut(s) 154, 949, 1153
BstMWI GCNNNNNNNGC 6 cut(s) 49, 581, 635, 776, 839, 848
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 2 cut(s) 618, 1136
BstSCI CCNGG 1 cut(s) 462
BstSFI CTRYAG 3 cut(s) 693, 1187, 1195
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 6 cut(s) 214, 294, 344, 700, 808, 826
BstV2I GAAGAC 2 cut(s) 6, 1104
BstXI CCANNNNNNTGG 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 355
BsuRI GGCC 1 cut(s) 833
BsuTUI ATCGAT 1 cut(s) 355
BtgI CCRYGG 1 cut(s) 542
BtsCI GGATG 2 cut(s) 416, 1104
BtsI GCAGTG 1 cut(s) 716
BtsIMutI CAGTG 3 cut(s) 117, 663, 716
BveI ACCTGC 3 cut(s) 670, 686, 1099
Cac8I GCNNGC 5 cut(s) 54, 280, 426, 469, 753
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 245, 698
ClaI ATCGAT 1 cut(s) 355
CseI GACGC 2 cut(s) 58, 768
Csp6I GTAC 3 cut(s) 409, 1062, 1223
CviAII CATG 3 cut(s) 543, 615, 1133
CviQI GTAC 3 cut(s) 409, 1062, 1223
DdeI CTNAG 5 cut(s) 257, 305, 318, 348, 578
DpnI GATC 3 cut(s) 156, 951, 1155
DpnII GATC 3 cut(s) 154, 949, 1153
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 906
EarI CTCTTC 1 cut(s) 906
Ecl136II GAGCTC 2 cut(s) 99, 575
Eco130I CCWWGG 2 cut(s) 528, 542
Eco24I GRGCYC 2 cut(s) 101, 577
Eco32I GATATC 1 cut(s) 979
Eco47I GGWCC 2 cut(s) 245, 698
Eco47III AGCGCT 1 cut(s) 206
Eco53kI GAGCTC 2 cut(s) 99, 575
Eco57I CTGAAG 3 cut(s) 71, 214, 1086
EcoICRI GAGCTC 2 cut(s) 99, 575
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 979
EcoT14I CCWWGG 2 cut(s) 528, 542
EcoT38I GRGCYC 2 cut(s) 101, 577
ErhI CCWWGG 2 cut(s) 528, 542
FaeI CATG 3 cut(s) 546, 618, 1136
FalI AAGNNNNNCTT 2 cut(s) 361, 393
FatI CATG 3 cut(s) 542, 614, 1132
FauI CCCGC 3 cut(s) 207, 562, 612
FauNDI CATATG 1 cut(s) 931
Fnu4HI GCNGC 9 cut(s) 190, 203, 276, 283, 286, 333, 714, 797, 840
FokI GGATG 2 cut(s) 403, 1091
FriOI GRGCYC 2 cut(s) 101, 577
Fsp4HI GCNGC 9 cut(s) 190, 203, 276, 283, 286, 333, 714, 797, 840
FspBI CTAG 1 cut(s) 936
GlaI GCGC 1 cut(s) 206
GluI GCNGC 9 cut(s) 190, 203, 276, 283, 286, 333, 714, 797, 840
GsaI CCCAGC 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 1 cut(s) 833
HapII CCGG 5 cut(s) 105, 763, 861, 869, 1108
HgaI GACGC 2 cut(s) 58, 768
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 2 cut(s) 8, 779
Hin1II CATG 3 cut(s) 546, 618, 1136
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 3 cut(s) 70, 160, 689
HpaII CCGG 5 cut(s) 105, 763, 861, 869, 1108
HphI GGTGA 2 cut(s) 545, 698
Hpy166II GTNNAC 1 cut(s) 701
Hpy188I TCNGA 7 cut(s) 159, 233, 260, 996, 1036, 1141, 1234
Hpy188III TCNNGA 7 cut(s) 46, 105, 164, 221, 452, 861, 869
Hpy8I GTNNAC 1 cut(s) 701
Hpy99I CGWCG 5 cut(s) 10, 13, 40, 139, 784
HpyCH4IV ACGT 2 cut(s) 8, 942
HpyCH4V TGCA 8 cut(s) 282, 629, 695, 716, 721, 791, 1073, 1094
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 581, 635, 776, 839, 848
HpyF3I CTNAG 5 cut(s) 257, 305, 318, 348, 578
HpySE526I ACGT 2 cut(s) 8, 942
Hsp92I GRCGYC 2 cut(s) 8, 779
Hsp92II CATG 3 cut(s) 546, 618, 1136
HspAI GCGC 1 cut(s) 205
Kpn2I TCCGGA 3 cut(s) 104, 860, 868
Kzo9I GATC 3 cut(s) 154, 949, 1153
LmnI GCTCC 6 cut(s) 96, 107, 572, 647, 887, 1222
Lsp1109I GCAGC 6 cut(s) 214, 294, 344, 700, 808, 826
LweI GCATC 4 cut(s) 30, 361, 826, 1113
MaeI CTAG 1 cut(s) 936
MaeII ACGT 2 cut(s) 8, 942
MaeIII GTNAC 1 cut(s) 844
MalI GATC 3 cut(s) 156, 951, 1155
MboI GATC 3 cut(s) 154, 949, 1153
MboII GAAGA 5 cut(s) 6, 77, 923, 1018, 1104
MhlI GDGCHC 2 cut(s) 101, 577
MlyI GAGTC 2 cut(s) 154, 698
MmeI TCCRAC 1 cut(s) 625
MnlI CCTC 6 cut(s) 107, 133, 399, 897, 907, 1018
MroI TCCGGA 3 cut(s) 104, 860, 868
MseI TTAA 4 cut(s) 498, 506, 597, 945
MspA1I CMGCKG 3 cut(s) 91, 285, 335
MspI CCGG 5 cut(s) 105, 763, 861, 869, 1108
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 6 cut(s) 49, 581, 635, 776, 839, 848
NcoI CCATGG 1 cut(s) 542
NdeI CATATG 1 cut(s) 931
NdeII GATC 3 cut(s) 154, 949, 1153
NlaIII CATG 3 cut(s) 546, 618, 1136
NlaIV GGNNCC 4 cut(s) 109, 247, 1052, 1089
NspI RCATGY 2 cut(s) 618, 1136
PaqCI CACCTGC 1 cut(s) 1099
PciI ACATGT 1 cut(s) 614
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 219
PfeI GAWTC 1 cut(s) 70
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 9 cut(s) 191, 204, 277, 284, 287, 334, 715, 798, 841
PleI GAGTC 2 cut(s) 154, 697
PpsI GAGTC 2 cut(s) 154, 697
PscI ACATGT 1 cut(s) 614
PsiI TTATAA 1 cut(s) 657
Psp124BI GAGCTC 2 cut(s) 101, 577
Psp6I CCWGG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 4 cut(s) 109, 247, 1052, 1089
PspPI GGNCC 2 cut(s) 245, 698
PstI CTGCAG 1 cut(s) 697
PsyI GACNNNGTC 1 cut(s) 10
PvuII CAGCTG 2 cut(s) 91, 335
RsaI GTAC 3 cut(s) 410, 1063, 1224
RsaNI GTAC 3 cut(s) 409, 1062, 1223
SacI GAGCTC 2 cut(s) 101, 577
SaqAI TTAA 4 cut(s) 498, 506, 597, 945
SatI GCNGC 9 cut(s) 190, 203, 276, 283, 286, 333, 714, 797, 840
Sau3AI GATC 3 cut(s) 154, 949, 1153
Sau96I GGNCC 2 cut(s) 245, 698
SchI GAGTC 2 cut(s) 154, 698
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 2 cut(s) 101, 577
SfaNI GCATC 4 cut(s) 30, 361, 826, 1113
SfcI CTRYAG 3 cut(s) 693, 1187, 1195
SinI GGWCC 2 cut(s) 245, 698
SsiI CCGC 7 cut(s) 190, 200, 267, 276, 285, 569, 605
SspI AATATT 1 cut(s) 1147
SspMI CTAG 1 cut(s) 936
SstI GAGCTC 2 cut(s) 101, 577
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 2 cut(s) 528, 542
TaiI ACGT 2 cut(s) 11, 945
TaqI TCGA 6 cut(s) 153, 165, 222, 355, 435, 981
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 3 cut(s) 192, 278, 288
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 4 cut(s) 498, 506, 597, 945
Tru9I TTAA 4 cut(s) 498, 506, 597, 945
TscAI CASTG 3 cut(s) 124, 670, 723
TseI GCWGC 6 cut(s) 202, 282, 332, 713, 796, 839
TspDTI ATGAA 4 cut(s) 83, 492, 504, 924
TspGWI ACGGA 4 cut(s) 152, 258, 378, 410
TspRI CASTG 3 cut(s) 124, 670, 723
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 245, 698
XapI RAATTY 8 cut(s) 194, 215, 261, 400, 491, 599, 668, 864
XceI RCATGY 2 cut(s) 618, 1136
XspI CTAG 1 cut(s) 936
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.