MD15G1248700.v1.1

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004990
Physical Location & Seq
Forward (+)
20754330 .. 20758073
3744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1248700.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGACGCGACTTCCGATGAAGACGTCGTTTTCGTTCTCGTTCTCCTTCTTCTTCACGATGATGCTCTGGGCGCCGCTCTCTTATGGCTTTGTGAATCCCCGAACACTTCTTCAGCTGGAGAGCTCGTCGGGAAGCGGTGGCGGCAAGCACTTGACGAAGGAGGAGCTTTGGTTCGATCAGACACTCGACCACTTCTCGCCGTACGACCGCCGCAAATTCCCGCAGCGATACTACGAATTTCTCGACTACTTTAGACCTTCCGACGGACCCATTTTCCTCAAAATTTGCGGAGAATCCGCGTGTGGCGGCATCTCCACCGATTATCTGAGTGTGTTGGCTAAGAAGTTTGGGGCAGCTATCGTTTCTCCCGAGCATCGATACTATGGAAAGAGCTCTCCTTTCAAATCTTATACGACACAGAATTTGAAGTACTTGTCATCCAAGCAAGCGCTTTTCGATTTGGCGGCTTTTCGTGAGTTTTATCAGAATTGGTTAAATGTGAAGCTGAATAGAACCAAGGGTGACAATCCATGGTTCGTGTTTGGTGTTTCTTACTCGGGAGCTCTTAGTGCGTGGTTTCGTGTTAAGTTTCCGCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTGGAAGCGATTTATGATTTCACTGAATTTGACCGGCAGATTGGTGAGTCTGCAGGTCCACAATGTAAAGCTGCACTTCAAGAAACTACTCGTATCGTTGAACAAAGTCTTACAACTAATGGAAAAGCAATAAGGGCGTTGTTTGGTGCAAGCCAGCTTGATATTGATGGTGACTTCATGTATCTTCTGGCTGATGCTGCTGTTATAGCGTTTCAGTATGGAAATCCCGATAAATTATGTTCACCTCTTGTTCAAGCAAAGACGAATGGAGAGGATTTGGTGGAAGCGTATGCCAAATACGTTAAAGATTTTTACCTTGGTACTCTTGGCATTGACGTTGATACATATAACCAGAAACACTTGAAGAACACTGCTGTTAGTGAGGGCAGTTCTGATCGGTTGTGGTGGTTCCAAGTTTGTACTGAAGTTGCATATTTTCAGGTGGCGCCTGCAAATGATAGCATCAGGTCTTCAAAAGTCGACACAAGATACCATGAAGACCTTTGCAAAAATGTTTTTGGAGACGGCATCTATCCTGATGTTTTTGCAACAAACTTATACTATGGAGGCACAAAAATTGCAGGTTCAAAAATAGTTTTTACAAATGGCTCACAGGATCCCTGGCGTCATGCATCCAAACAAACTTCATCTCCAGATATGCCTTCGTACGTCATCTCTTGCCATAACTGTGGCCATGGAACTGATTTGAGAGGATGCCCTCAGTGGCCTTTAACCCTTGAAGGCAATTCCCAGAACTGCAGCAACCCTGATGCAGTCAACAAAGTAAGGCAACAAATTGTTGAACACATTGACTTGTGGCTGTCGGAATGCCAAGAAACTGATGTTTGA

Protein Analysis

493

Amino Acids

55.33

Weight (kDa)

6.09

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 60 - 446 1.9e-73 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 2 cut(s) 674, 1202
AccB1I GGYRCC 2 cut(s) 70, 1075
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 1110
AccII CGCG 2 cut(s) 7, 299
AclWI GGATC 2 cut(s) 1241, 1254
AcoI YGGCCR 1 cut(s) 1321
AcsI RAATTY 5 cut(s) 215, 236, 282, 421, 656
AcuI CTGAAG 2 cut(s) 95, 1074
AcyI GRCGYC 4 cut(s) 23, 71, 1076, 1255
AfaI GTAC 5 cut(s) 203, 431, 952, 1051, 1298
AfeI AGCGCT 1 cut(s) 450
AfiI CCNNNNNNNGG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 602
AjnI CCWGG 1 cut(s) 1250
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 9 cut(s) 115, 123, 166, 356, 393, 505, 563, 701, 787
AluI AGCT 9 cut(s) 115, 123, 166, 356, 393, 505, 563, 701, 787
Alw21I GWGCWC 3 cut(s) 125, 395, 565
Alw26I GTCTC 1 cut(s) 1146
AlwI GGATC 2 cut(s) 1241, 1254
Ama87I CYCGRG 2 cut(s) 368, 556
Aor51HI AGCGCT 1 cut(s) 450
AoxI GGCC 2 cut(s) 1321, 1355
ApeKI GCWGC 5 cut(s) 223, 353, 701, 827, 1389
ApoI RAATTY 5 cut(s) 215, 236, 282, 421, 656
ArsI GACNNNNNNTTYG 4 cut(s) 13, 45, 406, 438
AspLEI GCGC 3 cut(s) 73, 451, 1078
AspS9I GGNCC 2 cut(s) 266, 686
AsuHPI GGTGA 4 cut(s) 533, 686, 812, 864
AvaI CYCGRG 2 cut(s) 368, 556
AvaII GGWCC 2 cut(s) 266, 686
BalI TGGCCA 1 cut(s) 1323
BamHI GGATCC 1 cut(s) 1246
BanI GGYRCC 2 cut(s) 70, 1075
BanII GRGCYC 3 cut(s) 125, 395, 565
BbsI GAAGAC 3 cut(s) 26, 1092, 1134
Bbv12I GWGCWC 3 cut(s) 125, 395, 565
BbvI GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BccI CCATC 1 cut(s) 791
BceAI ACGGC 2 cut(s) 184, 1171
BciT130I CCWGG 1 cut(s) 1252
BcoDI GTCTC 1 cut(s) 1146
BfmI CTRYAG 3 cut(s) 624, 681, 1387
BfoI RGCGCY 3 cut(s) 74, 452, 1079
BfuAI ACCTGC 2 cut(s) 674, 1202
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 1 cut(s) 1252
Bme18I GGWCC 2 cut(s) 266, 686
BmeT110I CYCGRG 2 cut(s) 368, 556
BmgT120I GGNCC 2 cut(s) 266, 686
BmiI GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
BmrFI CCNGG 1 cut(s) 1252
BmsI GCATC 9 cut(s) 51, 318, 382, 814, 1101, 1167, 1271, 1334, 1390
BpiI GAAGAC 3 cut(s) 26, 1092, 1134
BpmI CTGGAG 2 cut(s) 137, 1266
Bsa29I ATCGAT 1 cut(s) 376
BsaHI GRCGYC 4 cut(s) 23, 71, 1076, 1255
BsaJI CCNNGG 5 cut(s) 516, 530, 946, 1250, 1324
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 155, 185, 1264, 1294
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse118I RCCGGY 1 cut(s) 663
BseBI CCWGG 1 cut(s) 1252
BseCI ATCGAT 1 cut(s) 376
BseDI CCNNGG 5 cut(s) 516, 530, 946, 1250, 1324
BseGI GGATG 3 cut(s) 437, 1262, 1349
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 317, 1364
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 2 cut(s) 7, 299
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 2 cut(s) 1323, 1357
BshNI GGYRCC 2 cut(s) 70, 1075
BshVI ATCGAT 1 cut(s) 376
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 3 cut(s) 125, 395, 565
BsiHKCI CYCGRG 2 cut(s) 368, 556
BsiSI CCGG 1 cut(s) 664
BsiWI CGTACG 2 cut(s) 201, 1296
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 1146
BsmBI CGTCTC 1 cut(s) 1146
BsmI GAATGC 1 cut(s) 1463
BsnI GGCC 2 cut(s) 1323, 1357
BsoBI CYCGRG 2 cut(s) 368, 556
Bsp1286I GDGCHC 3 cut(s) 125, 395, 565
Bsp143I GATC 3 cut(s) 175, 1024, 1246
Bsp19I CCATGG 2 cut(s) 530, 1324
BspANI GGCC 2 cut(s) 1323, 1357
BspCNI CTCAG 2 cut(s) 318, 1363
BspDI ATCGAT 1 cut(s) 376
BspFNI CGCG 2 cut(s) 7, 299
BspLI GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
BspMAI CTGCAG 3 cut(s) 628, 685, 1391
BspMI ACCTGC 2 cut(s) 674, 1202
BspPI GGATC 2 cut(s) 1241, 1254
BspT107I GGYRCC 2 cut(s) 70, 1075
BsrBI CCGCTC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 663
BssAI RCCGGY 1 cut(s) 663
BssECI CCNNGG 5 cut(s) 516, 530, 946, 1250, 1324
BssMI GATC 3 cut(s) 175, 1024, 1246
BssNI GRCGYC 4 cut(s) 23, 71, 1076, 1255
BssT1I CCWWGG 4 cut(s) 516, 530, 946, 1324
Bst2UI CCWGG 1 cut(s) 1252
Bst4CI ACNGT 1 cut(s) 1319
BstACI GRCGYC 4 cut(s) 23, 71, 1076, 1255
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 5 cut(s) 146, 447, 781, 785, 1080
BstDEI CTNAG 4 cut(s) 326, 339, 566, 1350
BstDSI CCRYGG 2 cut(s) 530, 1324
BstF5I GGATG 3 cut(s) 437, 1262, 1349
BstFNI CGCG 2 cut(s) 7, 299
BstH2I RGCGCY 3 cut(s) 74, 452, 1079
BstHHI GCGC 3 cut(s) 73, 451, 1078
BstKTI GATC 3 cut(s) 178, 1027, 1249
BstMAI GTCTC 1 cut(s) 1146
BstMBI GATC 3 cut(s) 175, 1024, 1246
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 7 cut(s) 70, 141, 569, 623, 764, 827, 836
BstNI CCWGG 1 cut(s) 1252
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 1 cut(s) 1250
BstSFI CTRYAG 3 cut(s) 624, 681, 1387
BstUI CGCG 2 cut(s) 7, 299
BstV1I GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BstV2I GAAGAC 3 cut(s) 26, 1092, 1134
BstX2I RGATCY 1 cut(s) 1246
BstXI CCANNNNNNTGG 1 cut(s) 1319
BstYI RGATCY 1 cut(s) 1246
Bsu15I ATCGAT 1 cut(s) 376
BsuRI GGCC 2 cut(s) 1323, 1357
BsuTUI ATCGAT 1 cut(s) 376
BtgI CCRYGG 2 cut(s) 530, 1324
BtsCI GGATG 3 cut(s) 437, 1262, 1349
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 3 cut(s) 651, 999, 1358
BveI ACCTGC 2 cut(s) 674, 1202
Cac8I GCNNGC 5 cut(s) 146, 447, 781, 785, 1080
CfoI GCGC 3 cut(s) 73, 451, 1078
Cfr10I RCCGGY 1 cut(s) 663
Cfr13I GGNCC 2 cut(s) 266, 686
ClaI ATCGAT 1 cut(s) 376
CseI GACGC 2 cut(s) 13, 1244
Csp6I GTAC 5 cut(s) 202, 430, 951, 1050, 1297
CviAII CATG 6 cut(s) 531, 603, 808, 1124, 1259, 1325
CviQI GTAC 5 cut(s) 202, 430, 951, 1050, 1297
DdeI CTNAG 4 cut(s) 326, 339, 566, 1350
DinI GGCGCC 2 cut(s) 72, 1077
DpnI GATC 3 cut(s) 177, 1026, 1248
DpnII GATC 3 cut(s) 175, 1024, 1246
EaeI YGGCCR 1 cut(s) 1321
Ecl136II GAGCTC 3 cut(s) 123, 393, 563
Eco130I CCWWGG 4 cut(s) 516, 530, 946, 1324
Eco24I GRGCYC 3 cut(s) 125, 395, 565
Eco47I GGWCC 2 cut(s) 266, 686
Eco47III AGCGCT 1 cut(s) 450
Eco53kI GAGCTC 3 cut(s) 123, 393, 563
Eco57I CTGAAG 2 cut(s) 95, 1074
Eco88I CYCGRG 2 cut(s) 368, 556
EcoICRI GAGCTC 3 cut(s) 123, 393, 563
EcoRII CCWGG 1 cut(s) 1250
EcoT14I CCWWGG 4 cut(s) 516, 530, 946, 1324
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 3 cut(s) 125, 395, 565
EgeI GGCGCC 2 cut(s) 72, 1077
EheI GGCGCC 2 cut(s) 72, 1077
ErhI CCWWGG 4 cut(s) 516, 530, 946, 1324
Esp3I CGTCTC 1 cut(s) 1146
FaeI CATG 6 cut(s) 534, 606, 811, 1127, 1262, 1328
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 6 cut(s) 530, 602, 807, 1123, 1258, 1324
FauI CCCGC 1 cut(s) 228
FblI GTMKAC 1 cut(s) 1110
FokI GGATG 3 cut(s) 424, 1249, 1356
FriOI GRGCYC 3 cut(s) 125, 395, 565
GlaI GCGC 3 cut(s) 72, 450, 1077
GsuI CTGGAG 2 cut(s) 137, 1266
HaeII RGCGCY 3 cut(s) 74, 452, 1079
HaeIII GGCC 2 cut(s) 1323, 1357
HapII CCGG 1 cut(s) 664
HgaI GACGC 2 cut(s) 13, 1244
HhaI GCGC 3 cut(s) 73, 451, 1078
Hin1I GRCGYC 4 cut(s) 23, 71, 1076, 1255
Hin1II CATG 6 cut(s) 534, 606, 811, 1127, 1262, 1328
Hin6I GCGC 3 cut(s) 71, 449, 1076
HinP1I GCGC 3 cut(s) 71, 449, 1076
HincII GTYRAC 2 cut(s) 1111, 1408
HindII GTYRAC 2 cut(s) 1111, 1408
HinfI GANTC 3 cut(s) 94, 293, 677
HpaII CCGG 1 cut(s) 664
HphI GGTGA 4 cut(s) 533, 686, 812, 864
Hpy166II GTNNAC 4 cut(s) 689, 872, 1111, 1408
Hpy188I TCNGA 7 cut(s) 15, 180, 262, 327, 486, 1024, 1456
Hpy8I GTNNAC 4 cut(s) 689, 872, 1111, 1408
Hpy99I CGWCG 3 cut(s) 28, 130, 266
HpyAV CCTTC 5 cut(s) 55, 151, 267, 1302, 1364
HpyCH4III ACNGT 1 cut(s) 1319
HpyCH4IV ACGT 4 cut(s) 23, 930, 966, 1299
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 141, 569, 623, 764, 827, 836
HpyF3I CTNAG 4 cut(s) 326, 339, 566, 1350
HpySE526I ACGT 4 cut(s) 23, 930, 966, 1299
Hsp92I GRCGYC 4 cut(s) 23, 71, 1076, 1255
Hsp92II CATG 6 cut(s) 534, 606, 811, 1127, 1262, 1328
HspAI GCGC 3 cut(s) 71, 449, 1076
KasI GGCGCC 2 cut(s) 70, 1075
Kzo9I GATC 3 cut(s) 175, 1024, 1246
LmnI GCTCC 2 cut(s) 163, 560
Lsp1109I GCAGC 5 cut(s) 235, 365, 688, 814, 1401
LweI GCATC 9 cut(s) 51, 318, 382, 814, 1101, 1167, 1271, 1334, 1390
MaeII ACGT 4 cut(s) 23, 930, 966, 1299
MaeIII GTNAC 2 cut(s) 521, 800
MalI GATC 3 cut(s) 177, 1026, 1248
MbiI CCGCTC 1 cut(s) 76
MboI GATC 3 cut(s) 175, 1024, 1246
MboII GAAGA 8 cut(s) 31, 40, 43, 101, 806, 1006, 1092, 1139
MflI RGATCY 1 cut(s) 1246
MhlI GDGCHC 3 cut(s) 125, 395, 565
MlsI TGGCCA 1 cut(s) 1323
MluNI TGGCCA 1 cut(s) 1323
Mly113I GGCGCC 2 cut(s) 71, 1076
MlyI GAGTC 1 cut(s) 686
MmeI TCCRAC 2 cut(s) 285, 1434
MnlI CCTC 8 cut(s) 154, 287, 885, 895, 1006, 1190, 1334, 1359
Mox20I TGGCCA 1 cut(s) 1323
Mph1103I ATGCAT 1 cut(s) 1264
MscI TGGCCA 1 cut(s) 1323
MseI TTAA 4 cut(s) 494, 585, 933, 1361
MslI CAYNNNNRTG 2 cut(s) 59, 1317
Msp20I TGGCCA 1 cut(s) 1323
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 1 cut(s) 664
MspR9I CCNGG 1 cut(s) 1252
Mva1269I GAATGC 1 cut(s) 1463
MvaI CCWGG 1 cut(s) 1252
MvnI CGCG 2 cut(s) 7, 299
MwoI GCNNNNNNNGC 7 cut(s) 70, 141, 569, 623, 764, 827, 836
NarI GGCGCC 2 cut(s) 71, 1076
NcoI CCATGG 2 cut(s) 530, 1324
NdeII GATC 3 cut(s) 175, 1024, 1246
NlaIII CATG 6 cut(s) 534, 606, 811, 1127, 1262, 1328
NlaIV GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
NmuCI GTSAC 2 cut(s) 521, 800
NsiI ATGCAT 1 cut(s) 1264
NspI RCATGY 1 cut(s) 606
PciI ACATGT 1 cut(s) 602
PcsI WCGNNNNNNNCGW 4 cut(s) 11, 29, 240, 366
PctI GAATGC 1 cut(s) 1463
PfeI GAWTC 2 cut(s) 94, 293
Pfl23II CGTACG 2 cut(s) 201, 1296
PleI GAGTC 1 cut(s) 685
PluTI GGCGCC 2 cut(s) 74, 1079
PpsI GAGTC 1 cut(s) 685
PscI ACATGT 1 cut(s) 602
Psp124BI GAGCTC 3 cut(s) 125, 395, 565
Psp6I CCWGG 1 cut(s) 1250
PspGI CCWGG 1 cut(s) 1250
PspLI CGTACG 2 cut(s) 201, 1296
PspN4I GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
PspPI GGNCC 2 cut(s) 266, 686
PstI CTGCAG 3 cut(s) 628, 685, 1391
PsuI RGATCY 1 cut(s) 1246
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 5 cut(s) 203, 431, 952, 1051, 1298
RsaNI GTAC 5 cut(s) 202, 430, 951, 1050, 1297
RseI CAYNNNNRTG 2 cut(s) 59, 1317
SacI GAGCTC 3 cut(s) 125, 395, 565
SalI GTCGAC 1 cut(s) 1109
SaqAI TTAA 4 cut(s) 494, 585, 933, 1361
Sau3AI GATC 3 cut(s) 175, 1024, 1246
Sau96I GGNCC 2 cut(s) 266, 686
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 1 cut(s) 686
ScrFI CCNGG 1 cut(s) 1252
SduI GDGCHC 3 cut(s) 125, 395, 565
SfaNI GCATC 9 cut(s) 51, 318, 382, 814, 1101, 1167, 1271, 1334, 1390
SfcI CTRYAG 3 cut(s) 624, 681, 1387
SfoI GGCGCC 2 cut(s) 72, 1077
SinI GGWCC 2 cut(s) 266, 686
SmiMI CAYNNNNRTG 2 cut(s) 59, 1317
SspDI GGCGCC 2 cut(s) 70, 1075
SstI GAGCTC 3 cut(s) 125, 395, 565
StyD4I CCNGG 1 cut(s) 1250
StyI CCWWGG 4 cut(s) 516, 530, 946, 1324
TaaI ACNGT 1 cut(s) 1319
TaiI ACGT 4 cut(s) 26, 933, 969, 1302
TaqI TCGA 6 cut(s) 174, 186, 243, 376, 456, 1110
TatI WGTACW 2 cut(s) 429, 1049
TauI GCSGC 5 cut(s) 76, 144, 213, 309, 467
TfiI GAWTC 2 cut(s) 94, 293
Tru1I TTAA 4 cut(s) 494, 585, 933, 1361
Tru9I TTAA 4 cut(s) 494, 585, 933, 1361
TscAI CASTG 3 cut(s) 658, 1006, 1358
TseFI GTSAC 2 cut(s) 521, 800
TseI GCWGC 5 cut(s) 223, 353, 701, 827, 1389
Tsp45I GTSAC 2 cut(s) 521, 800
TspDTI ATGAA 4 cut(s) 32, 796, 1140, 1266
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 3 cut(s) 658, 1006, 1358
VpaK11BI GGWCC 2 cut(s) 266, 686
XapI RAATTY 5 cut(s) 215, 236, 282, 421, 656
XceI RCATGY 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 1110
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.