AT4G36190

serine protease EDA2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004990
Physical Location & Seq
Forward (+)
17123966 .. 17127018
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G36190.1

Sequence Viewer

Length: 1449 bp
ATGTCGTCGGAGCTTGGCTTTGCTTTACTTACCACCTTCACGGTGCTCTTGTCGTATTTAAGCTTTTCTAATGGGTTACTGCAACCTCGTAGGATTTCTCATGGATTGTCCAAAAGTAGCAAGTACTTGACGAGGGATGAGCTCTGGTTCACTCAAACCTTAGATCACTACTCTCCATCTGATCATCGCAAATTCAGACAGAGATACTATGAATATCTTGACCATTTACGAGTTCCTGACGGACCTATCTTTCTCATGATTTGCGGCGAAGGACCTTGCAATGGAATCACCAATAACTATATTAGTGTGTTGGCAAAGAAGTTTGATGCAGGCATTGTTTCACTCGAGCATCGTTACTATGGAAAGAGCTCACCATTTAAGTCGTTAGCCACTAAGAATCTCAAGTATCTTTCTTCCAAACAGGCCCTTTCCGATTTAGCTACTTTCCGCCAATATTACCAGGACTCTTTGAATGTTAAGTTTAATAGAAGCAGCAATGTTGAGAATCCGTGGTTTTTCTTTGGAGTATCTTATTCTGGAGCTTTAAGTGCTTGGTTCCGGCTTAAGTTCCCTCATTTGACCTGTGGAAGCCTCGCTAGTTCAGCAGTTGTTCGTGCTGTCTACGAATTCCCCGAATTTGATCAACAGATAGCTGAGTCGGCTGGACCGGAATGTGAAACTGCGCTACAAGAGACTAATAAACTCTTGGAACTAGGGCTTAAAGTGAATAACAGGGCTGTTAAAGCTCTCTTCAATGCCACGGAGCTTGATGTTGATGCTGATTTCTTATACTTAATAGCGGATGCAGGAGTTATGGCGATTCAATACGGAAACCCAGATAAACTATGTGTACCACTCGTGGAAGCTCAAAAGAATGGTGGTGATTTAGTGGAAGCATATGCTAAATATGTTAGAGAGTTTTGCATGGGAGTTTTCGGGCAAAGCTCTAAAACATATAGCCGGAAACATCTACTAGACACTGCGGTCACCCTAGAGAGTGCAGATAGGCTTTGGTGGTTCCAAGTTTGTACTGAAGTGGCTTATTTCCAGGTGGCACCTGCAAACGATAGCATCCGGTCTCATCAGATCAATACAGAGTATCATTTGGATCTATGCAAGAGCCTCTTTGGGAAAGGTGTCTACCCTGAAGTCGATGCAACAAACTTGTACTATGGAAGCGATAAAATTGCTGCGACCAAGATTATCTTCACAAATGGGTCACAGGATCCATGGCGTCATGCATCCAAACAAACCTCATCTCCTGACCTGCCTTCGTACATCATGACTTGTCACAACTGTGGGCATGGAAGTGATCTTCGAGGATGCCCCCAATCTGCAATGGTCATTGAAGGTGATGCCCAGAATTGCAGTTCACCAGATGCAGTGAACAAAGTGAGGCAACATATGATAAAACACATCGACTTGTGGCTCTCTGAGTGTCGTGGATAG

Protein Analysis

482

Amino Acids

54.21

Weight (kDa)

6.7

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 52 - 440 2.8e-73 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1066
AasI GACNNNNNNGTC 1 cut(s) 983
Acc36I ACCTGC 2 cut(s) 1066, 1275
AccB1I GGYRCC 1 cut(s) 1054
AccI GTMKAC 2 cut(s) 621, 1140
AciI CCGC 4 cut(s) 264, 448, 800, 983
AclWI GGATC 3 cut(s) 1116, 1220, 1233
AcsI RAATTY 3 cut(s) 191, 626, 635
AcuI CTGAAG 2 cut(s) 1053, 1167
AcyI GRCGYC 1 cut(s) 1234
AfaI GTAC 5 cut(s) 125, 852, 1030, 1169, 1277
AfiI CCNNNNNNNGG 1 cut(s) 281
AflII CTTAAG 1 cut(s) 563
AgsI TTSAA 4 cut(s) 472, 754, 824, 1349
AjnI CCWGG 2 cut(s) 459, 1047
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AleI CACNNNNGTG 1 cut(s) 1296
Alw21I GWGCWC 3 cut(s) 48, 144, 371
Alw26I GTCTC 2 cut(s) 686, 1083
AlwI GGATC 3 cut(s) 1116, 1220, 1233
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 2 cut(s) 492, 1190
ApoI RAATTY 3 cut(s) 191, 626, 635
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 4 cut(s) 242, 272, 424, 665
AsuHPI GGTGA 6 cut(s) 280, 363, 893, 979, 1364, 1365
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 3 cut(s) 242, 272, 665
BamHI GGATCC 1 cut(s) 1225
BanI GGYRCC 1 cut(s) 1054
BanII GRGCYC 2 cut(s) 144, 371
BauI CACGAG 1 cut(s) 857
Bbv12I GWGCWC 3 cut(s) 48, 144, 371
BbvI GCAGC 2 cut(s) 504, 1177
BccI CCATC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 1169, 1203
BciT130I CCWGG 2 cut(s) 461, 1049
BclI TGATCA 2 cut(s) 181, 640
BcoDI GTCTC 2 cut(s) 686, 1083
BfaI CTAG 4 cut(s) 597, 713, 974, 992
BfrI CTTAAG 1 cut(s) 563
BfuAI ACCTGC 2 cut(s) 1066, 1275
BisI GCNGC 3 cut(s) 265, 493, 1191
BlsI GCNGC 3 cut(s) 266, 494, 1192
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 461, 1049
Bme18I GGWCC 3 cut(s) 242, 272, 665
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 4 cut(s) 242, 272, 424, 665
BmiI GGNNCC 4 cut(s) 557, 1019, 1056, 1227
BmrFI CCNGG 2 cut(s) 461, 1049
BpmI CTGGAG 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 1234
BsaI GGTCTC 1 cut(s) 1083
BsaJI CCNNGG 3 cut(s) 509, 759, 1229
BsaWI WCCGGW 2 cut(s) 667, 1074
BsaXI ACNNNNNCTCC 2 cut(s) 1243, 1273
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse3DI GCAATG 3 cut(s) 286, 502, 1344
BseBI CCWGG 2 cut(s) 461, 1049
BseDI CCNNGG 3 cut(s) 509, 759, 1229
BseGI GGATG 5 cut(s) 142, 808, 1071, 1241, 1328
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 3 cut(s) 286, 502, 1344
BseMII CTCAG 2 cut(s) 645, 1425
BseXI GCAGC 2 cut(s) 504, 1177
BsgI GTGCAG 1 cut(s) 1020
BshFI GGCC 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 1054
BsiHKAI GWGCWC 3 cut(s) 48, 144, 371
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 4 cut(s) 559, 668, 961, 1075
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 2 cut(s) 686, 1083
BsnI GGCC 1 cut(s) 425
Bso31I GGTCTC 1 cut(s) 1083
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 3 cut(s) 48, 144, 371
Bsp143I GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
Bsp19I CCATGG 1 cut(s) 1229
BspACI CCGC 4 cut(s) 264, 448, 800, 983
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 2 cut(s) 646, 1426
BspHI TCATGA 2 cut(s) 255, 1281
BspLI GGNNCC 4 cut(s) 557, 1019, 1056, 1227
BspMI ACCTGC 2 cut(s) 1066, 1275
BspPI GGATC 3 cut(s) 1116, 1220, 1233
BspT107I GGYRCC 1 cut(s) 1054
BspTI CTTAAG 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 1083
BsrDI GCAATG 3 cut(s) 286, 502, 1344
BssECI CCNNGG 3 cut(s) 509, 759, 1229
BssMI GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
BssNI GRCGYC 1 cut(s) 1234
BssSI CACGAG 1 cut(s) 857
BssT1I CCWWGG 1 cut(s) 1229
Bst2BI CACGAG 1 cut(s) 857
Bst2UI CCWGG 2 cut(s) 461, 1049
Bst4CI ACNGT 2 cut(s) 43, 1298
Bst6I CTCTTC 1 cut(s) 755
BstACI GRCGYC 1 cut(s) 1234
BstAFI CTTAAG 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 4 cut(s) 160, 393, 654, 1434
BstDSI CCRYGG 3 cut(s) 509, 759, 1229
BstEII GGTNACC 1 cut(s) 985
BstF5I GGATG 5 cut(s) 142, 808, 1071, 1241, 1328
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 7 cut(s) 166, 184, 643, 1089, 1111, 1228, 1315
BstMAI GTCTC 2 cut(s) 686, 1083
BstMBI GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
BstMWI GCNNNNNNNGC 4 cut(s) 548, 602, 659, 743
BstNI CCWGG 2 cut(s) 461, 1049
BstPI GGTNACC 1 cut(s) 985
BstSCI CCNGG 2 cut(s) 459, 1047
BstV1I GCAGC 2 cut(s) 504, 1177
BstX2I RGATCY 2 cut(s) 1108, 1225
BstYI RGATCY 2 cut(s) 1108, 1225
BsuRI GGCC 1 cut(s) 425
BtgI CCRYGG 3 cut(s) 509, 759, 1229
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 5 cut(s) 142, 808, 1071, 1241, 1328
BtsI GCAGTG 2 cut(s) 978, 1389
BtsIMutI CAGTG 2 cut(s) 978, 1389
BveI ACCTGC 2 cut(s) 1066, 1275
Cac8I GCNNGC 1 cut(s) 331
CciI TCATGA 2 cut(s) 255, 1281
CfoI GCGC 1 cut(s) 685
Cfr13I GGNCC 4 cut(s) 242, 272, 424, 665
CseI GACGC 1 cut(s) 1223
Csp6I GTAC 5 cut(s) 124, 851, 1029, 1168, 1276
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 7 cut(s) 101, 256, 925, 1230, 1238, 1282, 1304
CviQI GTAC 5 cut(s) 124, 851, 1029, 1168, 1276
DdeI CTNAG 4 cut(s) 160, 393, 654, 1434
DpnI GATC 7 cut(s) 165, 183, 642, 1088, 1110, 1227, 1314
DpnII GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
DrdI GACNNNNNNGTC 1 cut(s) 983
DseDI GACNNNNNNGTC 1 cut(s) 983
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
EciI GGCGGA 1 cut(s) 437
Ecl136II GAGCTC 2 cut(s) 142, 369
Eco130I CCWWGG 1 cut(s) 1229
Eco24I GRGCYC 2 cut(s) 144, 371
Eco31I GGTCTC 1 cut(s) 1083
Eco47I GGWCC 3 cut(s) 242, 272, 665
Eco53kI GAGCTC 2 cut(s) 142, 369
Eco57I CTGAAG 2 cut(s) 1053, 1167
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 985
EcoICRI GAGCTC 2 cut(s) 142, 369
EcoO109I RGGNCCY 2 cut(s) 272, 424
EcoO65I GGTNACC 1 cut(s) 985
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 2 cut(s) 459, 1047
EcoT14I CCWWGG 1 cut(s) 1229
EcoT22I ATGCAT 1 cut(s) 1243
EcoT38I GRGCYC 2 cut(s) 144, 371
ErhI CCWWGG 1 cut(s) 1229
FaeI CATG 7 cut(s) 104, 259, 928, 1233, 1241, 1285, 1307
FalI AAGNNNNNCTT 4 cut(s) 1109, 1141, 1190, 1222
FatI CATG 7 cut(s) 100, 255, 924, 1229, 1237, 1281, 1303
FauNDI CATATG 2 cut(s) 898, 1404
FbaI TGATCA 2 cut(s) 181, 640
FblI GTMKAC 2 cut(s) 621, 1140
Fnu4HI GCNGC 3 cut(s) 265, 493, 1191
FokI GGATG 5 cut(s) 149, 815, 1058, 1228, 1335
FriOI GRGCYC 2 cut(s) 144, 371
Fsp4HI GCNGC 3 cut(s) 265, 493, 1191
FspBI CTAG 4 cut(s) 597, 713, 974, 992
GlaI GCGC 1 cut(s) 684
GluI GCNGC 3 cut(s) 265, 493, 1191
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 1 cut(s) 425
HapII CCGG 4 cut(s) 559, 668, 961, 1075
HgaI GACGC 1 cut(s) 1223
HhaI GCGC 1 cut(s) 685
Hin1I GRCGYC 1 cut(s) 1234
Hin1II CATG 7 cut(s) 104, 259, 928, 1233, 1241, 1285, 1307
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 6 cut(s) 285, 397, 464, 505, 656, 820
HpaII CCGG 4 cut(s) 559, 668, 961, 1075
HphI GGTGA 6 cut(s) 280, 363, 893, 979, 1364, 1365
Hpy166II GTNNAC 6 cut(s) 150, 622, 851, 1141, 1373, 1387
Hpy188I TCNGA 6 cut(s) 10, 181, 197, 433, 1086, 1435
Hpy188III TCNNGA 6 cut(s) 218, 236, 256, 537, 1262, 1282
Hpy8I GTNNAC 6 cut(s) 150, 622, 851, 1141, 1373, 1387
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 46, 263, 1281, 1343
HpyCH4III ACNGT 2 cut(s) 43, 1298
HpyF10VI GCNNNNNNNGC 4 cut(s) 548, 602, 659, 743
HpyF3I CTNAG 4 cut(s) 160, 393, 654, 1434
Hsp92I GRCGYC 1 cut(s) 1234
Hsp92II CATG 7 cut(s) 104, 259, 928, 1233, 1241, 1285, 1307
HspAI GCGC 1 cut(s) 683
Ksp22I TGATCA 2 cut(s) 181, 640
Kzo9I GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
LmnI GCTCC 3 cut(s) 10, 539, 763
Lsp1109I GCAGC 2 cut(s) 504, 1177
MaeI CTAG 4 cut(s) 597, 713, 974, 992
MaeIII GTNAC 5 cut(s) 75, 353, 985, 1218, 1289
MalI GATC 7 cut(s) 165, 183, 642, 1088, 1110, 1227, 1314
MboI GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
MboII GAAGA 4 cut(s) 405, 742, 1198, 1307
MflI RGATCY 2 cut(s) 1108, 1225
MhlI GDGCHC 3 cut(s) 48, 144, 371
MluCI AATT 5 cut(s) 191, 626, 635, 1185, 1363
MlyI GAGTC 2 cut(s) 458, 665
MnlI CCTC 8 cut(s) 96, 126, 582, 602, 1133, 1264, 1313, 1389
Mph1103I ATGCAT 1 cut(s) 1243
MseI TTAA 9 cut(s) 59, 378, 477, 483, 545, 564, 720, 741, 794
MslI CAYNNNNRTG 2 cut(s) 1296, 1308
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 4 cut(s) 559, 668, 961, 1075
MspR9I CCNGG 2 cut(s) 461, 1049
MvaI CCWGG 2 cut(s) 461, 1049
MwoI GCNNNNNNNGC 4 cut(s) 548, 602, 659, 743
NcoI CCATGG 1 cut(s) 1229
NdeI CATATG 2 cut(s) 898, 1404
NdeII GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
NlaIII CATG 7 cut(s) 104, 259, 928, 1233, 1241, 1285, 1307
NlaIV GGNNCC 4 cut(s) 557, 1019, 1056, 1227
NmuCI GTSAC 3 cut(s) 985, 1218, 1289
NsiI ATGCAT 1 cut(s) 1243
OliI CACNNNNGTG 1 cut(s) 1296
PaeR7I CTCGAG 1 cut(s) 344
PagI TCATGA 2 cut(s) 255, 1281
PaqCI CACCTGC 1 cut(s) 1066
PcsI WCGNNNNNNNCGW 1 cut(s) 630
PfeI GAWTC 4 cut(s) 285, 397, 505, 820
PkrI GCNGC 3 cut(s) 266, 494, 1192
PleI GAGTC 2 cut(s) 458, 664
PpsI GAGTC 2 cut(s) 458, 664
PpuMI RGGWCCY 1 cut(s) 272
Psp124BI GAGCTC 2 cut(s) 144, 371
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 459, 1047
PspEI GGTNACC 1 cut(s) 985
PspGI CCWGG 2 cut(s) 459, 1047
PspN4I GGNNCC 4 cut(s) 557, 1019, 1056, 1227
PspPI GGNCC 4 cut(s) 242, 272, 424, 665
PspPPI RGGWCCY 1 cut(s) 272
PspXI VCTCGAGB 1 cut(s) 344
PsuI RGATCY 2 cut(s) 1108, 1225
RsaI GTAC 5 cut(s) 125, 852, 1030, 1169, 1277
RsaNI GTAC 5 cut(s) 124, 851, 1029, 1168, 1276
RseI CAYNNNNRTG 2 cut(s) 1296, 1308
SacI GAGCTC 2 cut(s) 144, 371
SaqAI TTAA 9 cut(s) 59, 378, 477, 483, 545, 564, 720, 741, 794
SatI GCNGC 3 cut(s) 265, 493, 1191
Sau3AI GATC 7 cut(s) 163, 181, 640, 1086, 1108, 1225, 1312
Sau96I GGNCC 4 cut(s) 242, 272, 424, 665
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 2 cut(s) 458, 665
ScrFI CCNGG 2 cut(s) 461, 1049
SduI GDGCHC 3 cut(s) 48, 144, 371
Sfr274I CTCGAG 1 cut(s) 344
SinI GGWCC 3 cut(s) 242, 272, 665
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 1296, 1308
SmlI CTYRAG 3 cut(s) 344, 401, 563
SmoI CTYRAG 3 cut(s) 344, 401, 563
Sse9I AATT 5 cut(s) 191, 626, 635, 1185, 1363
SsiI CCGC 4 cut(s) 264, 448, 800, 983
SspI AATATT 1 cut(s) 455
SspMI CTAG 4 cut(s) 597, 713, 974, 992
SstI GAGCTC 2 cut(s) 144, 371
StyD4I CCNGG 2 cut(s) 459, 1047
StyI CCWWGG 1 cut(s) 1229
TaaI ACNGT 2 cut(s) 43, 1298
TaqI TCGA 4 cut(s) 345, 1152, 1318, 1419
TasI AATT 5 cut(s) 191, 626, 635, 1185, 1363
TatI WGTACW 3 cut(s) 123, 1028, 1167
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 4 cut(s) 285, 397, 505, 820
Tru1I TTAA 9 cut(s) 59, 378, 477, 483, 545, 564, 720, 741, 794
Tru9I TTAA 9 cut(s) 59, 378, 477, 483, 545, 564, 720, 741, 794
TscAI CASTG 2 cut(s) 985, 1389
TseFI GTSAC 3 cut(s) 985, 1218, 1289
TseI GCWGC 2 cut(s) 492, 1190
Tsp45I GTSAC 3 cut(s) 985, 1218, 1289
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 4 cut(s) 255, 498, 776, 843
TspRI CASTG 2 cut(s) 985, 1389
Vha464I CTTAAG 1 cut(s) 563
VpaK11BI GGWCC 3 cut(s) 242, 272, 665
XapI RAATTY 3 cut(s) 191, 626, 635
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 621, 1140
XspI CTAG 4 cut(s) 597, 713, 974, 992
ZrmI AGTACT 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.