Rw2G010270

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004990
Physical Location & Seq
Forward (+)
10379564 .. 10384111
4548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010270.1

Sequence Viewer

Length: 1500 bp
ATGCGGTTATACATTTCCGGCCAATCGGCGATGAAGCTAGCTCCGATGACGACGCCGTTTTTTCTCTCCGTCTTCTCAATCATCATACTCTGGAAGACGTCGTTTGCGTTCACGCCTCGGACTTTTCTTCAGCTCCAGAGCTCCGCCGTGAGCACCAATTACTTGACCGATGAGGAGCTCTGGTTCACTCAGACTCTCGATCACTTCTCGGCGTACGATCACCGCAAGTTCGAGCAGCGATATTATGAGCACCTCGACTTTTTCAGACCATCCGACGGTCCGATTTTCCTCAAAATCTGTGGAGAATCGGCCTGCAATGGAATCTCCAATGATTACTTAACTGTTTTGGCTAAGAAGTTTGGCGCGGCTGTGGTCTCGCTCGAGCATCGTTACTATGGTAAGAGCTCTCCGTTTAAATCACACTCGACTGAGAATTTGAAGTACTTATCATCGAAGCAAGCGCTTTTCGATTTGGCTGCTTTTCGTCAGTATTATCAGGAGTCGTTGAATTTGAAGCTGAATAAGACCAAGGGTGAGAATGCGTGGTTCGTATTTGGGGTGTCGTATCCAGGAGCTCTTAGTGCATGGTTTAGACTCAAGTTTCCTCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTTGAAGCTGTGTTTAACTACACTGAATTTGACCAGCAGATTGGTGAGTCTGCCGGTCCAGAATGTAAAGCTGCATTGCAAGAAACTAATCGTCTTGTTGAACAAAGGCTTGCAGCTAATGGAAAAGCAGTGCGGGGTTTGTTTGGTGCAACCCAGCTTGATATTGATGGTGATTTTATGTATTTTCTGGCGGATGCTGCTGTTATTGCGTTTCAATATGGAAATCCTGACAGACTGTGCTCCCCTCTTGTTCAAGCAAAGACTAATGGAGAGGATTTGGTTGAGGCATATGCCAAATATGTTAAAGAGTATTACCTTGGAAGTTTTGGTGTTGATGTCGAAACATACAATCAGAAACACTTGAAGAACACTGCTGTTACCGAGGGGAGTTCTGATCGTTTGTGGTGGTTCCAAGTTTGTACCGAAGTTGCCTATTTTCAGGTGGCACCTGCAAATGATAGCATCCGGTCTTCAAAAGTTGACACAAAATACCATTTGGACCTTTGCAAGAATGTCTTTGGAGAGGGCATCTATCCAGAGGTTGATGCGACAAATTTATATTATGGAGGCAAAAGAATTGCAGGTTCAAAAATAGTTTTCACAAATGGCTCACAGGATCCCTGGCGTCATGCATCCAAACAGACTTCATCTCCAGATATGCCTTCATACATCATCTCTTGCCATAATTGTGGCCATGGAACTGATTTGAGAGGATGTCCACAGGTTCCTTTAAGTCCCGAAGGTAATGCTGAGAACTGCAGCAGCCCTGATGCAGTCCACAAAGTTAGACAACAAATTGTAGAACACATAGACCTGTGGCTGTCTGAATGCCAAGAGACTGGTAGGAGCTACATGTAA

Protein Analysis

499

Amino Acids

56.12

Weight (kDa)

6.13

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 64 - 450 4.9e-76 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1099
AatII GACGTC 1 cut(s) 101
Acc36I ACCTGC 2 cut(s) 1099, 1214
AccB1I GGYRCC 1 cut(s) 1087
AccII CGCG 1 cut(s) 365
AciI CCGC 6 cut(s) 4, 144, 223, 365, 775, 833
AclWI GGATC 2 cut(s) 1253, 1266
AcoI YGGCCR 2 cut(s) 19, 1333
AcsI RAATTY 4 cut(s) 433, 508, 668, 1195
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 3 cut(s) 53, 98, 1267
AfaI GTAC 3 cut(s) 215, 443, 1063
AfeI AGCGCT 1 cut(s) 462
AfiI CCNNNNNNNGG 1 cut(s) 275
AflIII ACRYGT 2 cut(s) 614, 1494
AjnI CCWGG 2 cut(s) 568, 1262
AloI GAACNNNNNNTCC 2 cut(s) 167, 199
Alw21I GWGCWC 7 cut(s) 143, 155, 180, 252, 407, 577, 884
Alw26I GTCTC 2 cut(s) 379, 1472
AlwI GGATC 2 cut(s) 1253, 1266
Ama87I CYCGRG 1 cut(s) 380
Aor51HI AGCGCT 1 cut(s) 462
AoxI GGCC 3 cut(s) 19, 309, 1333
ApeKI GCWGC 7 cut(s) 235, 476, 713, 755, 839, 1401, 1404
ApoI RAATTY 4 cut(s) 433, 508, 668, 1195
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
AspLEI GCGC 2 cut(s) 365, 463
AspS9I GGNCC 3 cut(s) 278, 698, 1141
AsuHPI GGTGA 4 cut(s) 212, 545, 698, 824
AsuNHI GCTAGC 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 3 cut(s) 278, 698, 1141
BalI TGGCCA 1 cut(s) 1335
BamHI GGATCC 1 cut(s) 1258
BanI GGYRCC 1 cut(s) 1087
BanII GRGCYC 4 cut(s) 143, 180, 407, 577
BbsI GAAGAC 3 cut(s) 64, 101, 1104
Bbv12I GWGCWC 7 cut(s) 143, 155, 180, 252, 407, 577, 884
BbvI GCAGC 7 cut(s) 247, 463, 700, 767, 826, 1413, 1416
BccI CCATC 2 cut(s) 277, 803
BceAI ACGGC 2 cut(s) 40, 131
BcgI CGANNNNNNTGC 4 cut(s) 458, 492, 1370, 1404
BciT130I CCWGG 2 cut(s) 570, 1264
BciVI GTATCC 1 cut(s) 576
BcoDI GTCTC 2 cut(s) 379, 1472
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 636, 1399
BfoI RGCGCY 1 cut(s) 464
BfuAI ACCTGC 2 cut(s) 1099, 1214
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 8 cut(s) 236, 366, 477, 714, 756, 840, 1402, 1405
BlsI GCNGC 8 cut(s) 237, 367, 478, 715, 757, 841, 1403, 1406
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 2 cut(s) 570, 1264
Bme18I GGWCC 3 cut(s) 278, 698, 1141
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 3 cut(s) 278, 698, 1141
BmiI GGNNCC 4 cut(s) 1052, 1089, 1260, 1368
BmrFI CCNGG 2 cut(s) 570, 1264
BmsI GCATC 7 cut(s) 394, 826, 1113, 1177, 1179, 1283, 1402
BmtI GCTAGC 1 cut(s) 41
BpiI GAAGAC 3 cut(s) 64, 101, 1104
BpmI CTGGAG 2 cut(s) 119, 1278
BpuEI CTTGAG 1 cut(s) 581
BsaHI GRCGYC 3 cut(s) 53, 98, 1267
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 6 cut(s) 116, 528, 958, 1023, 1262, 1336
BsaWI WCCGGW 1 cut(s) 1107
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 1276, 1306
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse118I RCCGGY 1 cut(s) 695
Bse1I ACTGG 1 cut(s) 1486
Bse3DI GCAATG 2 cut(s) 322, 716
BseBI CCWGG 2 cut(s) 570, 1264
BseDI CCNNGG 6 cut(s) 116, 528, 958, 1023, 1262, 1336
BseGI GGATG 5 cut(s) 269, 841, 1104, 1274, 1361
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMI GCAATG 2 cut(s) 322, 716
BseMII CTCAG 3 cut(s) 203, 420, 1383
BseNI ACTGG 1 cut(s) 1486
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 7 cut(s) 247, 463, 700, 767, 826, 1413, 1416
BseYI CCCAGC 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 3 cut(s) 21, 311, 1335
BshNI GGYRCC 1 cut(s) 1087
BsiHKAI GWGCWC 7 cut(s) 143, 155, 180, 252, 407, 577, 884
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 18, 696, 1108
BsiWI CGTACG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 1362
BslI CCNNNNNNNGG 1 cut(s) 275
BsmAI GTCTC 2 cut(s) 379, 1472
BsmFI GGGAC 1 cut(s) 1362
BsmI GAATGC 2 cut(s) 544, 1475
BsnI GGCC 3 cut(s) 21, 311, 1335
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 7 cut(s) 143, 155, 180, 252, 407, 577, 884
Bsp143I GATC 4 cut(s) 199, 217, 1036, 1258
Bsp19I CCATGG 1 cut(s) 1336
BspACI CCGC 6 cut(s) 4, 144, 223, 365, 775, 833
BspANI GGCC 3 cut(s) 21, 311, 1335
BspCNI CTCAG 3 cut(s) 202, 421, 1384
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 4 cut(s) 1052, 1089, 1260, 1368
BspMAI CTGCAG 2 cut(s) 640, 1403
BspMI ACCTGC 2 cut(s) 1099, 1214
BspOI GCTAGC 1 cut(s) 41
BspPI GGATC 2 cut(s) 1253, 1266
BspT107I GGYRCC 1 cut(s) 1087
BspTNI GGTCTC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 322, 716
BsrFI RCCGGY 1 cut(s) 695
BsrI ACTGG 1 cut(s) 1486
BssAI RCCGGY 1 cut(s) 695
BssECI CCNNGG 6 cut(s) 116, 528, 958, 1023, 1262, 1336
BssMI GATC 4 cut(s) 199, 217, 1036, 1258
BssNI GRCGYC 3 cut(s) 53, 98, 1267
BssT1I CCWWGG 3 cut(s) 528, 958, 1336
Bst2UI CCWGG 2 cut(s) 570, 1264
Bst4CI ACNGT 3 cut(s) 278, 343, 879
BstACI GRCGYC 3 cut(s) 53, 98, 1267
BstAPI GCANNNNNTGC 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 39, 313, 459, 753
BstDEI CTNAG 5 cut(s) 189, 351, 429, 578, 1392
BstDSI CCRYGG 1 cut(s) 1336
BstF5I GGATG 5 cut(s) 269, 841, 1104, 1274, 1361
BstFNI CGCG 1 cut(s) 365
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 2 cut(s) 365, 463
BstKTI GATC 4 cut(s) 202, 220, 1039, 1261
BstMAI GTCTC 2 cut(s) 379, 1472
BstMBI GATC 4 cut(s) 199, 217, 1036, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 581, 635, 839, 848
BstNI CCWGG 2 cut(s) 570, 1264
BstNSI RCATGY 2 cut(s) 618, 1498
BstSCI CCNGG 2 cut(s) 568, 1262
BstSFI CTRYAG 2 cut(s) 636, 1399
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 7 cut(s) 247, 463, 700, 767, 826, 1413, 1416
BstV2I GAAGAC 3 cut(s) 64, 101, 1104
BstX2I RGATCY 1 cut(s) 1258
BstXI CCANNNNNNTGG 3 cut(s) 683, 1331, 1481
BstYI RGATCY 1 cut(s) 1258
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 3 cut(s) 21, 311, 1335
BtgI CCRYGG 1 cut(s) 1336
BtgZI GCGATG 1 cut(s) 44
BtsCI GGATG 5 cut(s) 269, 841, 1104, 1274, 1361
BtsI GCAGTG 2 cut(s) 777, 1011
BtsIMutI CAGTG 3 cut(s) 663, 777, 1011
BveI ACCTGC 2 cut(s) 1099, 1214
Cac8I GCNNGC 4 cut(s) 39, 313, 459, 753
CfoI GCGC 2 cut(s) 365, 463
Cfr10I RCCGGY 1 cut(s) 695
Cfr13I GGNCC 3 cut(s) 278, 698, 1141
CpoI CGGWCCG 1 cut(s) 278
CseI GACGC 2 cut(s) 61, 1256
Csp6I GTAC 3 cut(s) 214, 442, 1062
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CspI CGGWCCG 1 cut(s) 278
CviAII CATG 5 cut(s) 585, 615, 1271, 1337, 1495
CviQI GTAC 3 cut(s) 214, 442, 1062
DdeI CTNAG 5 cut(s) 189, 351, 429, 578, 1392
DpnI GATC 4 cut(s) 201, 219, 1038, 1260
DpnII GATC 4 cut(s) 199, 217, 1036, 1258
DraI TTTAAA 1 cut(s) 415
EaeI YGGCCR 2 cut(s) 19, 1333
EciI GGCGGA 2 cut(s) 133, 848
Ecl136II GAGCTC 4 cut(s) 141, 178, 405, 575
Eco130I CCWWGG 3 cut(s) 528, 958, 1336
Eco24I GRGCYC 4 cut(s) 143, 180, 407, 577
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 3 cut(s) 278, 698, 1141
Eco47III AGCGCT 1 cut(s) 462
Eco53kI GAGCTC 4 cut(s) 141, 178, 405, 575
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 380
EcoICRI GAGCTC 4 cut(s) 141, 178, 405, 575
EcoRII CCWGG 2 cut(s) 568, 1262
EcoT14I CCWWGG 3 cut(s) 528, 958, 1336
EcoT22I ATGCAT 1 cut(s) 1276
EcoT38I GRGCYC 4 cut(s) 143, 180, 407, 577
ErhI CCWWGG 3 cut(s) 528, 958, 1336
FaeI CATG 5 cut(s) 588, 618, 1274, 1340, 1498
FalI AAGNNNNNCTT 2 cut(s) 1142, 1174
FaqI GGGAC 1 cut(s) 1362
FatI CATG 5 cut(s) 584, 614, 1270, 1336, 1494
FauI CCCGC 1 cut(s) 768
FauNDI CATATG 1 cut(s) 931
Fnu4HI GCNGC 8 cut(s) 236, 366, 477, 714, 756, 840, 1402, 1405
FokI GGATG 5 cut(s) 256, 848, 1091, 1261, 1368
FriOI GRGCYC 4 cut(s) 143, 180, 407, 577
Fsp4HI GCNGC 8 cut(s) 236, 366, 477, 714, 756, 840, 1402, 1405
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 364, 462
GluI GCNGC 8 cut(s) 236, 366, 477, 714, 756, 840, 1402, 1405
GsaI CCCAGC 1 cut(s) 799
GsuI CTGGAG 2 cut(s) 119, 1278
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 3 cut(s) 21, 311, 1335
HapII CCGG 3 cut(s) 18, 696, 1108
HgaI GACGC 2 cut(s) 61, 1256
HhaI GCGC 2 cut(s) 365, 463
Hin1I GRCGYC 3 cut(s) 53, 98, 1267
Hin1II CATG 5 cut(s) 588, 618, 1274, 1340, 1498
Hin6I GCGC 2 cut(s) 363, 461
HinP1I GCGC 2 cut(s) 363, 461
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HinfI GANTC 6 cut(s) 193, 305, 321, 500, 594, 689
HpaII CCGG 3 cut(s) 18, 696, 1108
HphI GGTGA 4 cut(s) 212, 545, 698, 824
Hpy166II GTNNAC 5 cut(s) 111, 186, 1123, 1361, 1420
Hpy188I TCNGA 9 cut(s) 45, 120, 192, 266, 274, 282, 996, 1036, 1468
Hpy188III TCNNGA 9 cut(s) 91, 136, 197, 497, 701, 869, 1178, 1295, 1379
Hpy8I GTNNAC 5 cut(s) 111, 186, 1123, 1361, 1420
Hpy99I CGWCG 3 cut(s) 55, 103, 278
HpyAV CCTTC 2 cut(s) 1314, 1376
HpyCH4III ACNGT 3 cut(s) 278, 343, 879
HpyCH4IV ACGT 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 635, 839, 848
HpyF3I CTNAG 5 cut(s) 189, 351, 429, 578, 1392
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 3 cut(s) 53, 98, 1267
Hsp92II CATG 5 cut(s) 588, 618, 1274, 1340, 1498
HspAI GCGC 2 cut(s) 363, 461
Kzo9I GATC 4 cut(s) 199, 217, 1036, 1258
LmnI GCTCC 7 cut(s) 46, 138, 146, 175, 572, 887, 1488
Lsp1109I GCAGC 7 cut(s) 247, 463, 700, 767, 826, 1413, 1416
LweI GCATC 7 cut(s) 394, 826, 1113, 1177, 1179, 1283, 1402
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 389, 1018
MalI GATC 4 cut(s) 201, 219, 1038, 1260
MboI GATC 4 cut(s) 199, 217, 1036, 1258
MboII GAAGA 5 cut(s) 64, 106, 119, 1018, 1104
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 7 cut(s) 143, 155, 180, 252, 407, 577, 884
MlsI TGGCCA 1 cut(s) 1335
MluCI AATT 8 cut(s) 157, 433, 508, 668, 1195, 1218, 1327, 1437
MluNI TGGCCA 1 cut(s) 1335
MlyI GAGTC 4 cut(s) 187, 509, 588, 698
MmeI TCCRAC 1 cut(s) 297
Mox20I TGGCCA 1 cut(s) 1335
Mph1103I ATGCAT 1 cut(s) 1276
MscI TGGCCA 1 cut(s) 1335
MseI TTAA 5 cut(s) 338, 414, 657, 945, 1373
MslI CAYNNNNRTG 1 cut(s) 1329
Msp20I TGGCCA 1 cut(s) 1335
MspI CCGG 3 cut(s) 18, 696, 1108
MspR9I CCNGG 2 cut(s) 570, 1264
Mva1269I GAATGC 2 cut(s) 544, 1475
MvaI CCWGG 2 cut(s) 570, 1264
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 4 cut(s) 581, 635, 839, 848
NcoI CCATGG 1 cut(s) 1336
NdeI CATATG 1 cut(s) 931
NdeII GATC 4 cut(s) 199, 217, 1036, 1258
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 5 cut(s) 588, 618, 1274, 1340, 1498
NlaIV GGNNCC 4 cut(s) 1052, 1089, 1260, 1368
NmeAIII GCCGAG 1 cut(s) 188
NsiI ATGCAT 1 cut(s) 1276
NspI RCATGY 2 cut(s) 618, 1498
PaeR7I CTCGAG 1 cut(s) 380
PaqCI CACCTGC 1 cut(s) 1099
PciI ACATGT 2 cut(s) 614, 1494
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PctI GAATGC 2 cut(s) 544, 1475
PfeI GAWTC 2 cut(s) 305, 321
Pfl23II CGTACG 1 cut(s) 213
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 8 cut(s) 237, 367, 478, 715, 757, 841, 1403, 1406
PleI GAGTC 4 cut(s) 187, 508, 588, 697
PpsI GAGTC 4 cut(s) 187, 508, 588, 697
PscI ACATGT 2 cut(s) 614, 1494
Psp124BI GAGCTC 4 cut(s) 143, 180, 407, 577
Psp6I CCWGG 2 cut(s) 568, 1262
PspFI CCCAGC 1 cut(s) 795
PspGI CCWGG 2 cut(s) 568, 1262
PspLI CGTACG 1 cut(s) 213
PspN4I GGNNCC 4 cut(s) 1052, 1089, 1260, 1368
PspPI GGNCC 3 cut(s) 278, 698, 1141
PspXI VCTCGAGB 1 cut(s) 380
PstI CTGCAG 2 cut(s) 640, 1403
PsuI RGATCY 1 cut(s) 1258
RsaI GTAC 3 cut(s) 215, 443, 1063
RsaNI GTAC 3 cut(s) 214, 442, 1062
RseI CAYNNNNRTG 1 cut(s) 1329
Rsr2I CGGWCCG 1 cut(s) 278
RsrII CGGWCCG 1 cut(s) 278
SacI GAGCTC 4 cut(s) 143, 180, 407, 577
SaqAI TTAA 5 cut(s) 338, 414, 657, 945, 1373
SatI GCNGC 8 cut(s) 236, 366, 477, 714, 756, 840, 1402, 1405
Sau3AI GATC 4 cut(s) 199, 217, 1036, 1258
Sau96I GGNCC 3 cut(s) 278, 698, 1141
ScaI AGTACT 1 cut(s) 443
SchI GAGTC 4 cut(s) 187, 509, 588, 698
ScrFI CCNGG 2 cut(s) 570, 1264
SduI GDGCHC 7 cut(s) 143, 155, 180, 252, 407, 577, 884
SfaNI GCATC 7 cut(s) 394, 826, 1113, 1177, 1179, 1283, 1402
SfcI CTRYAG 2 cut(s) 636, 1399
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 3 cut(s) 278, 698, 1141
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 1329
SmlI CTYRAG 2 cut(s) 380, 596
SmoI CTYRAG 2 cut(s) 380, 596
Sse9I AATT 8 cut(s) 157, 433, 508, 668, 1195, 1218, 1327, 1437
SsiI CCGC 6 cut(s) 4, 144, 223, 365, 775, 833
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 4 cut(s) 143, 180, 407, 577
StyD4I CCNGG 2 cut(s) 568, 1262
StyI CCWWGG 3 cut(s) 528, 958, 1336
TaaI ACNGT 3 cut(s) 278, 343, 879
TaiI ACGT 1 cut(s) 101
TaqI TCGA 8 cut(s) 198, 231, 255, 381, 425, 452, 468, 981
TaqII GACCGA 1 cut(s) 182
TasI AATT 8 cut(s) 157, 433, 508, 668, 1195, 1218, 1327, 1437
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 2 cut(s) 305, 321
Tru1I TTAA 5 cut(s) 338, 414, 657, 945, 1373
Tru9I TTAA 5 cut(s) 338, 414, 657, 945, 1373
TscAI CASTG 3 cut(s) 670, 777, 1018
TseI GCWGC 7 cut(s) 235, 476, 713, 755, 839, 1401, 1404
TspDTI ATGAA 3 cut(s) 47, 1278, 1296
TspGWI ACGGA 2 cut(s) 58, 399
TspRI CASTG 3 cut(s) 670, 777, 1018
VpaK11BI GGWCC 3 cut(s) 278, 698, 1141
XapI RAATTY 4 cut(s) 433, 508, 668, 1195
XceI RCATGY 2 cut(s) 618, 1498
XhoI CTCGAG 1 cut(s) 380
XspI CTAG 1 cut(s) 38
ZraI GACGTC 1 cut(s) 99
ZrmI AGTACT 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.