AT4G36195

serine protease EDA2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004990
Physical Location & Seq
Forward (+)
17126932 .. 17130023
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G36195.3

Sequence Viewer

Length: 1467 bp
ATGTTGTCGGCGCTTGGCTTTGCTTTACTCAGCATCTTCGCGATCCTCTTGTCGTTATCAACCTTGTCTAATGGATTACTTCAACCTCGTAGGATTTCTCATGGCTTGACGGAAAGTAGCAAGTACTTGACGAGGGATGAGCTCTGGTTCAATCAAACCCTAGATCATTACTCTCCTTCTGATCATCGCGAATTCAAACAGCGATACTATGAATATCTAGACCATTTACGAGTTCCTGATGGACCTATCTTTATGATGATCTGTGGCGAAGGACCTTGCAATGGAATCCCCAATGACTATATAACTGTGTTAGCAAAGAAGTTTGATGCAGGCATTGTTTCACTTGAGCATCGTTATTACGGAAAGAGCTCACCGTTTAAGTCATTAGCCACTGAGAATCTCAAGTATCTTTCTTCTAAACAGGCTCTTTTCGATTTAGCTGCTTTCCGCCAATATTACCAGGACTCTTTGAATGTTAAGTTTAATAGAAGTGGCGATGTTGAGAATCCGTGGTTTTTCTTCGGAGCTTCTTATTCTGGAGCTTTAAGTGCTTGGTTCCGGCTTAAGTTCCCTCATTTGACCTGTGGAAGCCTTGCTAGTTCTGCAGTTGTTCGTGCAGTCTACGAATTCCCTGAATTTGATCAACAGATAGGTGAGTCGGCTGGACCGGAATGTAAAGCTGCACTACAAGAGACTAATAAGCTCTTGGAACTAGGGCTTAAAGTGAATAACAGGGCTGTGAAAGCTCTCTTCAATGCCACCGAGCTTGACGTTGATGCTGATTTCTTATACTTGATAGCAGATGCAGAAGTTATGGCGATCCAATACGGAAACCCAGATAAACTATGTGTACCACTCGTGGAAGCTCAGAAGAATCGTGATGATTTAGTGGAAGCATATGCTAAATATGTTAGAGAGTTTTGTGTGGGGGTTTTCGGGCTAAGCTCTAAAACATATAGCCGGAAACATCTGCTAGACACTGCGGTCACCCCAGAAAGTGCAGATAGGCTTTGGTGGTTCCAAGTTTGCACTGAAGTGGCATATTTCCAAGTGGCACCTGCAAACGATAGCATCCGGTCTCATCAAATCAATACAGAGTATCATTTGGATCTATGCAAGAGTCTGTTTGGGAAAGGTGTTTACCCTGAAGTCGATGCAACAAACCTGTATTATGGAAGTGACAGAATTGCTGCGACCAAGATTATCTTCACAAATGGGTCACAGGATCCATGGCGTCATGCATCCAAACAGACCTCATCTCCTGAACTGCCTTCTTACATTGTGACTTGCCACAACTGTGGCCATGGAAGTGATCTCCGTGGATGCCCTCAATCTCCCATGGTCATTGGAGGTGATTCCAAGAATTGCAGTTCACCTGATGCAGTGAACAAAGTGAGGCAACATATAGTAGATCATATGGACTTGTGGCTCTCTGAGTGTCGTGGAGGGATTAGGAGTTCCATGTAA

Protein Analysis

488

Amino Acids

54.77

Weight (kDa)

6.07

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 52 - 440 2.7e-73 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1066
AasI GACNNNNNNGTC 1 cut(s) 983
Acc36I ACCTGC 1 cut(s) 1066
AccB1I GGYRCC 1 cut(s) 1054
AccI GTMKAC 1 cut(s) 621
AccII CGCG 2 cut(s) 41, 189
AciI CCGC 2 cut(s) 448, 983
AclWI GGATC 5 cut(s) 37, 814, 1116, 1220, 1233
AcoI YGGCCR 1 cut(s) 1300
AcsI RAATTY 3 cut(s) 191, 626, 635
AcuI CTGAAG 2 cut(s) 1053, 1167
AcyI GRCGYC 1 cut(s) 1234
AfaI GTAC 2 cut(s) 125, 852
AfiI CCNNNNNNNGG 1 cut(s) 281
AflII CTTAAG 1 cut(s) 563
AgsI TTSAA 5 cut(s) 83, 151, 196, 472, 754
AjnI CCWGG 1 cut(s) 459
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AleI CACNNNNGTG 2 cut(s) 1034, 1296
AloI GAACNNNNNNTCC 1 cut(s) 1441
Alw21I GWGCWC 2 cut(s) 144, 371
Alw26I GTCTC 2 cut(s) 686, 1083
AlwI GGATC 5 cut(s) 37, 814, 1116, 1220, 1233
AoxI GGCC 1 cut(s) 1300
ApeKI GCWGC 3 cut(s) 440, 680, 1190
ApoI RAATTY 3 cut(s) 191, 626, 635
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 3 cut(s) 242, 272, 665
AsuHPI GGTGA 5 cut(s) 363, 665, 979, 1364, 1365
AvaII GGWCC 3 cut(s) 242, 272, 665
BalI TGGCCA 1 cut(s) 1302
BamHI GGATCC 1 cut(s) 1225
BanI GGYRCC 1 cut(s) 1054
BanII GRGCYC 2 cut(s) 144, 371
BauI CACGAG 1 cut(s) 857
Bbv12I GWGCWC 2 cut(s) 144, 371
BbvI GCAGC 3 cut(s) 427, 667, 1177
BccI CCATC 1 cut(s) 233
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 1 cut(s) 461
BclI TGATCA 2 cut(s) 181, 640
BcoDI GTCTC 2 cut(s) 686, 1083
BfaI CTAG 5 cut(s) 161, 218, 597, 713, 974
BfmI CTRYAG 1 cut(s) 603
BfoI RGCGCY 1 cut(s) 14
BfrI CTTAAG 1 cut(s) 563
BfuAI ACCTGC 1 cut(s) 1066
BisI GCNGC 3 cut(s) 441, 681, 1191
BlpI GCTNAGC 1 cut(s) 941
BlsI GCNGC 3 cut(s) 442, 682, 1192
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 1 cut(s) 461
Bme18I GGWCC 3 cut(s) 242, 272, 665
BmgT120I GGNCC 3 cut(s) 242, 272, 665
BmiI GGNNCC 4 cut(s) 557, 1019, 1056, 1227
BmrFI CCNGG 1 cut(s) 461
BpmI CTGGAG 1 cut(s) 558
Bpu1102I GCTNAGC 1 cut(s) 941
BpuEI CTTGAG 2 cut(s) 365, 386
BsaHI GRCGYC 1 cut(s) 1234
BsaI GGTCTC 1 cut(s) 1083
BsaJI CCNNGG 5 cut(s) 509, 1229, 1303, 1318, 1338
BsaWI WCCGGW 2 cut(s) 667, 1074
BsaXI ACNNNNNCTCC 2 cut(s) 1243, 1273
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 5 cut(s) 509, 1229, 1303, 1318, 1338
BseGI GGATG 4 cut(s) 142, 1071, 1241, 1328
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 4 cut(s) 43, 384, 881, 1425
BseXI GCAGC 3 cut(s) 427, 667, 1177
BsgI GTGCAG 3 cut(s) 636, 666, 1020
Bsh1236I CGCG 2 cut(s) 41, 189
BshFI GGCC 1 cut(s) 1302
BshNI GGYRCC 1 cut(s) 1054
BsiHKAI GWGCWC 2 cut(s) 144, 371
BsiSI CCGG 4 cut(s) 559, 668, 961, 1075
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 2 cut(s) 686, 1083
BsnI GGCC 1 cut(s) 1302
Bso31I GGTCTC 1 cut(s) 1083
Bsp1286I GDGCHC 2 cut(s) 144, 371
Bsp1720I GCTNAGC 1 cut(s) 941
Bsp19I CCATGG 3 cut(s) 1229, 1303, 1338
Bsp68I TCGCGA 2 cut(s) 41, 189
BspACI CCGC 2 cut(s) 448, 983
BspANI GGCC 1 cut(s) 1302
BspCNI CTCAG 4 cut(s) 42, 385, 880, 1426
BspFNI CGCG 2 cut(s) 41, 189
BspLI GGNNCC 4 cut(s) 557, 1019, 1056, 1227
BspMAI CTGCAG 1 cut(s) 607
BspMI ACCTGC 1 cut(s) 1066
BspPI GGATC 5 cut(s) 37, 814, 1116, 1220, 1233
BspT107I GGYRCC 1 cut(s) 1054
BspTI CTTAAG 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 1083
BsrDI GCAATG 1 cut(s) 286
BssECI CCNNGG 5 cut(s) 509, 1229, 1303, 1318, 1338
BssNI GRCGYC 1 cut(s) 1234
BssSI CACGAG 1 cut(s) 857
BssT1I CCWWGG 3 cut(s) 1229, 1303, 1338
Bst2BI CACGAG 1 cut(s) 857
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 3 cut(s) 307, 375, 1298
Bst6I CTCTTC 1 cut(s) 755
BstACI GRCGYC 1 cut(s) 1234
BstAFI CTTAAG 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 5 cut(s) 29, 393, 867, 941, 1434
BstDSI CCRYGG 5 cut(s) 509, 1229, 1303, 1318, 1338
BstEII GGTNACC 1 cut(s) 985
BstF5I GGATG 4 cut(s) 142, 1071, 1241, 1328
BstFNI CGCG 2 cut(s) 41, 189
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 1 cut(s) 13
BstMAI GTCTC 2 cut(s) 686, 1083
BstMWI GCNNNNNNNGC 3 cut(s) 548, 602, 743
BstNI CCWGG 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 985
BstSCI CCNGG 1 cut(s) 459
BstSFI CTRYAG 1 cut(s) 603
BstUI CGCG 2 cut(s) 41, 189
BstV1I GCAGC 3 cut(s) 427, 667, 1177
BstX2I RGATCY 2 cut(s) 1108, 1225
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 2 cut(s) 1108, 1225
BsuRI GGCC 1 cut(s) 1302
BtgI CCRYGG 5 cut(s) 509, 1229, 1303, 1318, 1338
BtgZI GCGATG 2 cut(s) 170, 510
BtsCI GGATG 4 cut(s) 142, 1071, 1241, 1328
BtsI GCAGTG 2 cut(s) 978, 1389
BtsIMutI CAGTG 4 cut(s) 390, 978, 1029, 1389
BtuMI TCGCGA 2 cut(s) 41, 189
BveI ACCTGC 1 cut(s) 1066
Cac8I GCNNGC 1 cut(s) 331
CfoI GCGC 1 cut(s) 13
Cfr13I GGNCC 3 cut(s) 242, 272, 665
CseI GACGC 1 cut(s) 1223
Csp6I GTAC 2 cut(s) 124, 851
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 6 cut(s) 101, 1230, 1238, 1304, 1339, 1462
CviQI GTAC 2 cut(s) 124, 851
DdeI CTNAG 5 cut(s) 29, 393, 867, 941, 1434
DrdI GACNNNNNNGTC 1 cut(s) 983
DseDI GACNNNNNNGTC 1 cut(s) 983
EaeI YGGCCR 1 cut(s) 1300
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
EciI GGCGGA 1 cut(s) 437
Ecl136II GAGCTC 2 cut(s) 142, 369
Eco130I CCWWGG 3 cut(s) 1229, 1303, 1338
Eco24I GRGCYC 2 cut(s) 144, 371
Eco31I GGTCTC 1 cut(s) 1083
Eco47I GGWCC 3 cut(s) 242, 272, 665
Eco53kI GAGCTC 2 cut(s) 142, 369
Eco57I CTGAAG 2 cut(s) 1053, 1167
Eco91I GGTNACC 1 cut(s) 985
EcoICRI GAGCTC 2 cut(s) 142, 369
EcoO109I RGGNCCY 1 cut(s) 272
EcoO65I GGTNACC 1 cut(s) 985
EcoRI GAATTC 2 cut(s) 191, 626
EcoRII CCWGG 1 cut(s) 459
EcoT14I CCWWGG 3 cut(s) 1229, 1303, 1338
EcoT22I ATGCAT 1 cut(s) 1243
EcoT38I GRGCYC 2 cut(s) 144, 371
ErhI CCWWGG 3 cut(s) 1229, 1303, 1338
FaeI CATG 6 cut(s) 104, 1233, 1241, 1307, 1342, 1465
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FatI CATG 6 cut(s) 100, 1229, 1237, 1303, 1338, 1461
FauNDI CATATG 2 cut(s) 898, 1416
FbaI TGATCA 2 cut(s) 181, 640
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 3 cut(s) 441, 681, 1191
FokI GGATG 4 cut(s) 149, 1058, 1228, 1335
FriOI GRGCYC 2 cut(s) 144, 371
Fsp4HI GCNGC 3 cut(s) 441, 681, 1191
FspBI CTAG 5 cut(s) 161, 218, 597, 713, 974
GlaI GCGC 1 cut(s) 12
GluI GCNGC 3 cut(s) 441, 681, 1191
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 1 cut(s) 1302
HapII CCGG 4 cut(s) 559, 668, 961, 1075
HgaI GACGC 1 cut(s) 1223
HhaI GCGC 1 cut(s) 13
Hin1I GRCGYC 1 cut(s) 1234
Hin1II CATG 6 cut(s) 104, 1233, 1241, 1307, 1342, 1465
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HinfI GANTC 8 cut(s) 285, 397, 464, 505, 656, 874, 1120, 1355
HpaII CCGG 4 cut(s) 559, 668, 961, 1075
HphI GGTGA 5 cut(s) 363, 665, 979, 1364, 1365
Hpy166II GTNNAC 5 cut(s) 622, 851, 1141, 1373, 1387
Hpy188I TCNGA 4 cut(s) 181, 524, 870, 1435
Hpy188III TCNNGA 7 cut(s) 40, 188, 218, 236, 537, 878, 1262
Hpy8I GTNNAC 5 cut(s) 622, 851, 1141, 1373, 1387
HpyAV CCTTC 3 cut(s) 186, 263, 1281
HpyCH4III ACNGT 3 cut(s) 307, 375, 1298
HpyCH4IV ACGT 1 cut(s) 771
HpyF10VI GCNNNNNNNGC 3 cut(s) 548, 602, 743
HpyF3I CTNAG 5 cut(s) 29, 393, 867, 941, 1434
HpySE526I ACGT 1 cut(s) 771
Hsp92I GRCGYC 1 cut(s) 1234
Hsp92II CATG 6 cut(s) 104, 1233, 1241, 1307, 1342, 1465
HspAI GCGC 1 cut(s) 11
Ksp22I TGATCA 2 cut(s) 181, 640
LmnI GCTCC 2 cut(s) 524, 539
Lsp1109I GCAGC 3 cut(s) 427, 667, 1177
MaeI CTAG 5 cut(s) 161, 218, 597, 713, 974
MaeII ACGT 1 cut(s) 771
MaeIII GTNAC 4 cut(s) 985, 1178, 1218, 1282
MboII GAAGA 6 cut(s) 28, 405, 511, 742, 883, 1198
MflI RGATCY 2 cut(s) 1108, 1225
MhlI GDGCHC 2 cut(s) 144, 371
MlsI TGGCCA 1 cut(s) 1302
MluCI AATT 5 cut(s) 191, 626, 635, 1185, 1363
MluNI TGGCCA 1 cut(s) 1302
MlyI GAGTC 3 cut(s) 458, 665, 1129
MnlI CCTC 9 cut(s) 56, 96, 126, 582, 1264, 1338, 1343, 1389, 1439
Mox20I TGGCCA 1 cut(s) 1302
Mph1103I ATGCAT 1 cut(s) 1243
MscI TGGCCA 1 cut(s) 1302
MseI TTAA 6 cut(s) 378, 477, 483, 545, 564, 720
MslI CAYNNNNRTG 3 cut(s) 1034, 1296, 1308
Msp20I TGGCCA 1 cut(s) 1302
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 4 cut(s) 559, 668, 961, 1075
MspR9I CCNGG 1 cut(s) 461
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 2 cut(s) 41, 189
MwoI GCNNNNNNNGC 3 cut(s) 548, 602, 743
NcoI CCATGG 3 cut(s) 1229, 1303, 1338
NdeI CATATG 2 cut(s) 898, 1416
NlaIII CATG 6 cut(s) 104, 1233, 1241, 1307, 1342, 1465
NlaIV GGNNCC 4 cut(s) 557, 1019, 1056, 1227
NmuCI GTSAC 4 cut(s) 985, 1178, 1218, 1282
NruI TCGCGA 2 cut(s) 41, 189
NsiI ATGCAT 1 cut(s) 1243
OliI CACNNNNGTG 2 cut(s) 1034, 1296
PaqCI CACCTGC 1 cut(s) 1066
PfeI GAWTC 5 cut(s) 285, 397, 505, 874, 1355
PkrI GCNGC 3 cut(s) 442, 682, 1192
PleI GAGTC 3 cut(s) 458, 664, 1128
PpsI GAGTC 3 cut(s) 458, 664, 1128
PpuMI RGGWCCY 1 cut(s) 272
Psp124BI GAGCTC 2 cut(s) 144, 371
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 459
PspEI GGTNACC 1 cut(s) 985
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 4 cut(s) 557, 1019, 1056, 1227
PspPI GGNCC 3 cut(s) 242, 272, 665
PspPPI RGGWCCY 1 cut(s) 272
PstI CTGCAG 1 cut(s) 607
PsuI RGATCY 2 cut(s) 1108, 1225
RruI TCGCGA 2 cut(s) 41, 189
RsaI GTAC 2 cut(s) 125, 852
RsaNI GTAC 2 cut(s) 124, 851
RseI CAYNNNNRTG 3 cut(s) 1034, 1296, 1308
SacI GAGCTC 2 cut(s) 144, 371
SaqAI TTAA 6 cut(s) 378, 477, 483, 545, 564, 720
SatI GCNGC 3 cut(s) 441, 681, 1191
Sau96I GGNCC 3 cut(s) 242, 272, 665
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 3 cut(s) 458, 665, 1129
ScrFI CCNGG 1 cut(s) 461
SduI GDGCHC 2 cut(s) 144, 371
SfcI CTRYAG 1 cut(s) 603
SinI GGWCC 3 cut(s) 242, 272, 665
SmiMI CAYNNNNRTG 3 cut(s) 1034, 1296, 1308
SmlI CTYRAG 3 cut(s) 344, 401, 563
SmoI CTYRAG 3 cut(s) 344, 401, 563
Sse9I AATT 5 cut(s) 191, 626, 635, 1185, 1363
SsiI CCGC 2 cut(s) 448, 983
SspI AATATT 1 cut(s) 455
SspMI CTAG 5 cut(s) 161, 218, 597, 713, 974
SstI GAGCTC 2 cut(s) 144, 371
StyD4I CCNGG 1 cut(s) 459
StyI CCWWGG 3 cut(s) 1229, 1303, 1338
TaaI ACNGT 3 cut(s) 307, 375, 1298
TaiI ACGT 1 cut(s) 774
TaqI TCGA 2 cut(s) 432, 1152
TasI AATT 5 cut(s) 191, 626, 635, 1185, 1363
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 5 cut(s) 285, 397, 505, 874, 1355
Tru1I TTAA 6 cut(s) 378, 477, 483, 545, 564, 720
Tru9I TTAA 6 cut(s) 378, 477, 483, 545, 564, 720
TscAI CASTG 4 cut(s) 397, 985, 1036, 1389
TseFI GTSAC 4 cut(s) 985, 1178, 1218, 1282
TseI GCWGC 3 cut(s) 440, 680, 1190
Tsp45I GTSAC 4 cut(s) 985, 1178, 1218, 1282
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 5 cut(s) 125, 375, 498, 843, 1307
TspRI CASTG 4 cut(s) 397, 985, 1036, 1389
Vha464I CTTAAG 1 cut(s) 563
VpaK11BI GGWCC 3 cut(s) 242, 272, 665
XapI RAATTY 3 cut(s) 191, 626, 635
XbaI TCTAGA 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 621
XspI CTAG 5 cut(s) 161, 218, 597, 713, 974
ZrmI AGTACT 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.