pycom02g10670

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004990
Physical Location & Seq
Forward (+)
7410057 .. 7413128
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10670.1

Sequence Viewer

Length: 1488 bp
ATGACCCGATTTCCGATGATGACGTCGTTTTCGTTCTTCTTGTCCTTCTTCTTCACGATGATGATGTGGGCGCCGCTCTCTTATGGCTTTGTCAATCCGCGAACACTTCTTCAGCTGGAGAGCTCGTCGGGAAGCGGCGGCGGAAGGTTATTGACGAAGGAGGAGCTGTGGTTCGATCAGACTCTTGACCACTTCTCGCCGTACGACCGCCGCAAATTCCCGCAGCGCTACTACGAATTTCTCGACTACTTCAGACCTTCCGACGGACCAATTTTTCTCAAAATTTGCGGAGAATCCGCATGCGGCGGCATCGCCAACGATTACCTGAGTGTGTTGGCTAAGAAGTTTGGGGCAGCTATCGTTGCGCCCGAGCATCGATACTACGGAAAAAGCTCTCCTTTCAATACGCATACAACGCAGAATTTGAAGTACTTGTCATCCAAGCAAGCGCTTTTCGATCTGGCGGCTTTTCGTGAGTTTTATCAGAATGGGTTAAATGTGAAGCTGAATAGAACCAAGGGTGACAATCCGTGGTTCGTGTTTGGTATTTCTTACCCGGGAGCTCTTAGTGCGTGGTTTCGGGTTAAGTTCCCGCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTTGAAGCTATTTATAACTTCACTGAATTTGACCAGCAGATTGGTGAGTCTGCAGGTCCAGAATGTAAAGCTGCACTTCAAGAAACTACTCATCTTGTTGAACAAAGTCTCACAACTAATGGAAAAGCAATAAGGGCGTTGTTTGGTGCAAGCCAGCTTGATATTGATGGCGACTTCATGTATTTTCTGGCGGATGCTGCTGTTATAGCGTTTCAATATGGAAATCCCGATAAATTATGCTCACCTCTTGTTCAAGCAAAGACTAATGGAGAGGATTTGGTGGAAGCGTATGCCAAATACATTAAAGATTATTACCTTGGAACTTTTGGCATTGACGTTGATACATATAACCAGAAACACTTGAAGAACACTGCTGTTACTGAGGGCAGTTCTGATCGATTGTGGTGGTTCCAAGTTTGTACTGAAGTTGCATATTTTCAGGTGGCTCCTGCAAATGATAGCATCAGGTCTTCAAAAGTTGACACAAAATACCATGAAGACCTTTGCAAAAATGTTTTTGGAGACGGCATCTATCCTGACGTTCTTGCAACAAACTTATACTATGGAGGCACAAAAATTGCAGGTTCAAAAATAGTTTTTACAAATGGTTCACAGGACCCCTGGCGTCATGCATCTAAACAAACTTCATCTCCAGATATGCCTTCGTACATCATCTCTTGCCATAACTGTGGCCATGGAACGGATCTGAGAGGATGCCCTCAGTCTCCTTTAACCCTTGAAGGCAATTCCCAGAACTGCAGCAACCCTGATGCAGTCAACAAAGTAAGGCAACAGATTGTTGAACACATCGACTTGTGGCTATCTGAATGTCAAGAAGCTGGTAGGAGTTACATGTAA

Protein Analysis

496

Amino Acids

55.53

Weight (kDa)

5.91

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 60 - 447 3.6e-74 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 2 cut(s) 674, 1202
AccB1I GGYRCC 1 cut(s) 70
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 100
AclWI GGATC 1 cut(s) 1341
AcoI YGGCCR 1 cut(s) 1321
AcsI RAATTY 5 cut(s) 215, 236, 282, 421, 656
AcuI CTGAAG 3 cut(s) 95, 235, 1074
AcyI GRCGYC 3 cut(s) 23, 71, 1255
AfaI GTAC 4 cut(s) 203, 431, 1051, 1298
AfeI AGCGCT 2 cut(s) 227, 450
AfiI CCNNNNNNNGG 2 cut(s) 263, 1330
AflIII ACRYGT 2 cut(s) 602, 1482
AjnI CCWGG 1 cut(s) 1250
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
Alw21I GWGCWC 2 cut(s) 125, 565
Alw26I GTCTC 3 cut(s) 743, 1146, 1359
AlwI GGATC 1 cut(s) 1341
Ama87I CYCGRG 2 cut(s) 368, 556
Aor51HI AGCGCT 2 cut(s) 227, 450
AoxI GGCC 1 cut(s) 1321
ApeKI GCWGC 5 cut(s) 223, 353, 701, 827, 1389
ApoI RAATTY 5 cut(s) 215, 236, 282, 421, 656
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
AspLEI GCGC 4 cut(s) 73, 228, 367, 451
AspS9I GGNCC 3 cut(s) 266, 686, 1246
AsuC2I CCSGG 2 cut(s) 557, 558
AsuHPI GGTGA 3 cut(s) 533, 686, 864
AvaI CYCGRG 2 cut(s) 368, 556
AvaII GGWCC 3 cut(s) 266, 686, 1246
BalI TGGCCA 1 cut(s) 1323
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 2 cut(s) 125, 565
BbsI GAAGAC 2 cut(s) 1092, 1134
Bbv12I GWGCWC 2 cut(s) 125, 565
BbvI GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BccI CCATC 1 cut(s) 791
BceAI ACGGC 2 cut(s) 184, 1171
BciT130I CCWGG 1 cut(s) 1252
BcnI CCSGG 2 cut(s) 557, 558
BcoDI GTCTC 3 cut(s) 743, 1146, 1359
BfmI CTRYAG 3 cut(s) 624, 681, 1387
BfoI RGCGCY 3 cut(s) 74, 229, 452
BfuAI ACCTGC 2 cut(s) 674, 1202
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 3 cut(s) 557, 558, 1252
Bme18I GGWCC 3 cut(s) 266, 686, 1246
BmeT110I CYCGRG 2 cut(s) 368, 556
BmgT120I GGNCC 3 cut(s) 266, 686, 1246
BmiI GGNNCC 4 cut(s) 72, 1040, 1077, 1248
BmrFI CCNGG 3 cut(s) 557, 558, 1252
BmsI GCATC 8 cut(s) 318, 382, 814, 1101, 1167, 1271, 1334, 1390
BpiI GAAGAC 2 cut(s) 1092, 1134
BpmI CTGGAG 2 cut(s) 137, 1266
BpuMI CCSGG 2 cut(s) 557, 558
Bsa29I ATCGAT 2 cut(s) 376, 1027
BsaHI GRCGYC 3 cut(s) 23, 71, 1255
BsaJI CCNNGG 6 cut(s) 516, 530, 556, 946, 1250, 1324
BsaXI ACNNNNNCTCC 8 cut(s) 110, 140, 152, 155, 182, 185, 1264, 1294
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 1330
BseBI CCWGG 1 cut(s) 1252
BseCI ATCGAT 2 cut(s) 376, 1027
BseDI CCNNGG 6 cut(s) 516, 530, 556, 946, 1250, 1324
BseGI GGATG 3 cut(s) 437, 829, 1349
BseLI CCNNNNNNNGG 2 cut(s) 263, 1330
BseMII CTCAG 4 cut(s) 317, 1002, 1328, 1364
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 1323
BshNI GGYRCC 1 cut(s) 70
BshVI ATCGAT 2 cut(s) 376, 1027
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 125, 565
BsiHKCI CYCGRG 2 cut(s) 368, 556
BsiSI CCGG 1 cut(s) 557
BsiWI CGTACG 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 263, 1330
BsmAI GTCTC 3 cut(s) 743, 1146, 1359
BsmBI CGTCTC 1 cut(s) 1146
BsnI GGCC 1 cut(s) 1323
BsoBI CYCGRG 2 cut(s) 368, 556
Bsp1286I GDGCHC 2 cut(s) 125, 565
Bsp143I GATC 4 cut(s) 175, 457, 1024, 1333
Bsp19I CCATGG 1 cut(s) 1324
BspANI GGCC 1 cut(s) 1323
BspCNI CTCAG 4 cut(s) 318, 1003, 1329, 1363
BspDI ATCGAT 2 cut(s) 376, 1027
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 72, 1040, 1077, 1248
BspMAI CTGCAG 3 cut(s) 628, 685, 1391
BspMI ACCTGC 2 cut(s) 674, 1202
BspPI GGATC 1 cut(s) 1341
BspT107I GGYRCC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 76
BssECI CCNNGG 6 cut(s) 516, 530, 556, 946, 1250, 1324
BssMI GATC 4 cut(s) 175, 457, 1024, 1333
BssNI GRCGYC 3 cut(s) 23, 71, 1255
BssT1I CCWWGG 3 cut(s) 516, 946, 1324
Bst2UI CCWGG 1 cut(s) 1252
Bst4CI ACNGT 1 cut(s) 1319
BstACI GRCGYC 3 cut(s) 23, 71, 1255
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 4 cut(s) 301, 447, 781, 785
BstDEI CTNAG 6 cut(s) 326, 339, 566, 1011, 1337, 1350
BstDSI CCRYGG 2 cut(s) 530, 1324
BstF5I GGATG 3 cut(s) 437, 829, 1349
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 3 cut(s) 74, 229, 452
BstHHI GCGC 4 cut(s) 73, 228, 367, 451
BstKTI GATC 4 cut(s) 178, 460, 1027, 1336
BstMAI GTCTC 3 cut(s) 743, 1146, 1359
BstMBI GATC 4 cut(s) 175, 457, 1024, 1333
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 7 cut(s) 362, 415, 569, 623, 764, 827, 836
BstNI CCWGG 1 cut(s) 1252
BstNSI RCATGY 3 cut(s) 303, 606, 1486
BstSCI CCNGG 3 cut(s) 555, 556, 1250
BstSFI CTRYAG 3 cut(s) 624, 681, 1387
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BstV2I GAAGAC 2 cut(s) 1092, 1134
BstX2I RGATCY 1 cut(s) 1333
BstXI CCANNNNNNTGG 2 cut(s) 671, 1319
BstYI RGATCY 1 cut(s) 1333
Bsu15I ATCGAT 2 cut(s) 376, 1027
BsuRI GGCC 1 cut(s) 1323
BsuTUI ATCGAT 2 cut(s) 376, 1027
BtgI CCRYGG 2 cut(s) 530, 1324
BtgZI GCGATG 1 cut(s) 295
BtsCI GGATG 3 cut(s) 437, 829, 1349
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 2 cut(s) 651, 999
BveI ACCTGC 2 cut(s) 674, 1202
Cac8I GCNNGC 4 cut(s) 301, 447, 781, 785
CfoI GCGC 4 cut(s) 73, 228, 367, 451
Cfr13I GGNCC 3 cut(s) 266, 686, 1246
Cfr9I CCCGGG 1 cut(s) 556
ClaI ATCGAT 2 cut(s) 376, 1027
CseI GACGC 1 cut(s) 1244
Csp6I GTAC 4 cut(s) 202, 430, 1050, 1297
CviAII CATG 7 cut(s) 300, 603, 808, 1124, 1259, 1325, 1483
CviQI GTAC 4 cut(s) 202, 430, 1050, 1297
DdeI CTNAG 6 cut(s) 326, 339, 566, 1011, 1337, 1350
DinI GGCGCC 1 cut(s) 72
DpnI GATC 4 cut(s) 177, 459, 1026, 1335
DpnII GATC 4 cut(s) 175, 457, 1024, 1333
EaeI YGGCCR 1 cut(s) 1321
EciI GGCGGA 2 cut(s) 156, 836
Ecl136II GAGCTC 2 cut(s) 123, 563
Eco130I CCWWGG 3 cut(s) 516, 946, 1324
Eco24I GRGCYC 2 cut(s) 125, 565
Eco47I GGWCC 3 cut(s) 266, 686, 1246
Eco47III AGCGCT 2 cut(s) 227, 450
Eco53kI GAGCTC 2 cut(s) 123, 563
Eco57I CTGAAG 3 cut(s) 95, 235, 1074
Eco88I CYCGRG 2 cut(s) 368, 556
EcoICRI GAGCTC 2 cut(s) 123, 563
EcoO109I RGGNCCY 1 cut(s) 1246
EcoRII CCWGG 1 cut(s) 1250
EcoT14I CCWWGG 3 cut(s) 516, 946, 1324
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 2 cut(s) 125, 565
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 516, 946, 1324
Esp3I CGTCTC 1 cut(s) 1146
FaeI CATG 7 cut(s) 303, 606, 811, 1127, 1262, 1328, 1486
FalI AAGNNNNNCTT 4 cut(s) 382, 414, 690, 722
FatI CATG 7 cut(s) 299, 602, 807, 1123, 1258, 1324, 1482
FauI CCCGC 2 cut(s) 228, 600
FokI GGATG 3 cut(s) 424, 836, 1356
FriOI GRGCYC 2 cut(s) 125, 565
GlaI GCGC 4 cut(s) 72, 227, 366, 450
GsuI CTGGAG 2 cut(s) 137, 1266
HaeII RGCGCY 3 cut(s) 74, 229, 452
HaeIII GGCC 1 cut(s) 1323
HapII CCGG 1 cut(s) 557
HgaI GACGC 1 cut(s) 1244
HhaI GCGC 4 cut(s) 73, 228, 367, 451
Hin1I GRCGYC 3 cut(s) 23, 71, 1255
Hin1II CATG 7 cut(s) 303, 606, 811, 1127, 1262, 1328, 1486
Hin6I GCGC 4 cut(s) 71, 226, 365, 449
HinP1I GCGC 4 cut(s) 71, 226, 365, 449
HincII GTYRAC 2 cut(s) 1111, 1408
HindII GTYRAC 2 cut(s) 1111, 1408
HinfI GANTC 3 cut(s) 181, 293, 677
HpaII CCGG 1 cut(s) 557
HphI GGTGA 3 cut(s) 533, 686, 864
Hpy166II GTNNAC 3 cut(s) 1111, 1241, 1408
Hpy188I TCNGA 8 cut(s) 15, 180, 254, 262, 486, 1024, 1338, 1456
Hpy8I GTNNAC 3 cut(s) 1111, 1241, 1408
Hpy99I CGWCG 3 cut(s) 28, 130, 266
HpyAV CCTTC 6 cut(s) 55, 138, 151, 267, 1302, 1364
HpyCH4III ACNGT 1 cut(s) 1319
HpyCH4IV ACGT 3 cut(s) 23, 966, 1170
HpyF10VI GCNNNNNNNGC 7 cut(s) 362, 415, 569, 623, 764, 827, 836
HpyF3I CTNAG 6 cut(s) 326, 339, 566, 1011, 1337, 1350
HpySE526I ACGT 3 cut(s) 23, 966, 1170
Hsp92I GRCGYC 3 cut(s) 23, 71, 1255
Hsp92II CATG 7 cut(s) 303, 606, 811, 1127, 1262, 1328, 1486
HspAI GCGC 4 cut(s) 71, 226, 365, 449
KasI GGCGCC 1 cut(s) 70
Kzo9I GATC 4 cut(s) 175, 457, 1024, 1333
LmnI GCTCC 3 cut(s) 163, 560, 1081
Lsp1109I GCAGC 5 cut(s) 235, 365, 688, 814, 1401
LweI GCATC 8 cut(s) 318, 382, 814, 1101, 1167, 1271, 1334, 1390
MaeII ACGT 3 cut(s) 23, 966, 1170
MaeIII GTNAC 3 cut(s) 521, 1006, 1478
MalI GATC 4 cut(s) 177, 459, 1026, 1335
MbiI CCGCTC 1 cut(s) 76
MboI GATC 4 cut(s) 175, 457, 1024, 1333
MboII GAAGA 7 cut(s) 28, 40, 43, 101, 1006, 1092, 1139
MflI RGATCY 1 cut(s) 1333
MhlI GDGCHC 2 cut(s) 125, 565
MlsI TGGCCA 1 cut(s) 1323
MluCI AATT 9 cut(s) 215, 236, 270, 282, 421, 656, 863, 1206, 1375
MluNI TGGCCA 1 cut(s) 1323
Mly113I GGCGCC 1 cut(s) 71
MlyI GAGTC 2 cut(s) 175, 686
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 7 cut(s) 154, 885, 895, 1006, 1190, 1334, 1359
Mox20I TGGCCA 1 cut(s) 1323
Mph1103I ATGCAT 1 cut(s) 1264
MscI TGGCCA 1 cut(s) 1323
MseI TTAA 4 cut(s) 494, 585, 933, 1361
MslI CAYNNNNRTG 2 cut(s) 59, 1317
Msp20I TGGCCA 1 cut(s) 1323
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 3 cut(s) 557, 558, 1252
MvaI CCWGG 1 cut(s) 1252
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 7 cut(s) 362, 415, 569, 623, 764, 827, 836
NarI GGCGCC 1 cut(s) 71
NciI CCSGG 2 cut(s) 557, 558
NcoI CCATGG 1 cut(s) 1324
NdeII GATC 4 cut(s) 175, 457, 1024, 1333
NlaIII CATG 7 cut(s) 303, 606, 811, 1127, 1262, 1328, 1486
NlaIV GGNNCC 4 cut(s) 72, 1040, 1077, 1248
NmuCI GTSAC 1 cut(s) 521
NsiI ATGCAT 1 cut(s) 1264
NspI RCATGY 3 cut(s) 303, 606, 1486
PaeI GCATGC 1 cut(s) 303
PciI ACATGT 2 cut(s) 602, 1482
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 240, 366
PfeI GAWTC 1 cut(s) 293
Pfl23II CGTACG 1 cut(s) 201
PleI GAGTC 2 cut(s) 175, 685
PluTI GGCGCC 1 cut(s) 74
PpsI GAGTC 2 cut(s) 175, 685
PpuMI RGGWCCY 1 cut(s) 1246
PscI ACATGT 2 cut(s) 602, 1482
PsiI TTATAA 1 cut(s) 645
Psp124BI GAGCTC 2 cut(s) 125, 565
Psp5II RGGWCCY 1 cut(s) 1246
Psp6I CCWGG 1 cut(s) 1250
PspGI CCWGG 1 cut(s) 1250
PspLI CGTACG 1 cut(s) 201
PspN4I GGNNCC 4 cut(s) 72, 1040, 1077, 1248
PspPI GGNCC 3 cut(s) 266, 686, 1246
PspPPI RGGWCCY 1 cut(s) 1246
PstI CTGCAG 3 cut(s) 628, 685, 1391
PsuI RGATCY 1 cut(s) 1333
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 4 cut(s) 203, 431, 1051, 1298
RsaNI GTAC 4 cut(s) 202, 430, 1050, 1297
RseI CAYNNNNRTG 2 cut(s) 59, 1317
SacI GAGCTC 2 cut(s) 125, 565
SaqAI TTAA 4 cut(s) 494, 585, 933, 1361
Sau3AI GATC 4 cut(s) 175, 457, 1024, 1333
Sau96I GGNCC 3 cut(s) 266, 686, 1246
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 2 cut(s) 175, 686
ScrFI CCNGG 3 cut(s) 557, 558, 1252
SduI GDGCHC 2 cut(s) 125, 565
SfaNI GCATC 8 cut(s) 318, 382, 814, 1101, 1167, 1271, 1334, 1390
SfcI CTRYAG 3 cut(s) 624, 681, 1387
SfoI GGCGCC 1 cut(s) 72
SinI GGWCC 3 cut(s) 266, 686, 1246
SmaI CCCGGG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 59, 1317
SphI GCATGC 1 cut(s) 303
Sse9I AATT 9 cut(s) 215, 236, 270, 282, 421, 656, 863, 1206, 1375
SspDI GGCGCC 1 cut(s) 70
SstI GAGCTC 2 cut(s) 125, 565
StyD4I CCNGG 3 cut(s) 555, 556, 1250
StyI CCWWGG 3 cut(s) 516, 946, 1324
TaaI ACNGT 1 cut(s) 1319
TaiI ACGT 3 cut(s) 26, 969, 1173
TaqI TCGA 6 cut(s) 174, 243, 376, 456, 1027, 1440
TasI AATT 9 cut(s) 215, 236, 270, 282, 421, 656, 863, 1206, 1375
TatI WGTACW 2 cut(s) 429, 1049
TauI GCSGC 7 cut(s) 76, 138, 141, 213, 306, 309, 467
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 4 cut(s) 494, 585, 933, 1361
Tru9I TTAA 4 cut(s) 494, 585, 933, 1361
TscAI CASTG 2 cut(s) 658, 1006
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 5 cut(s) 223, 353, 701, 827, 1389
Tsp45I GTSAC 1 cut(s) 521
TspDTI ATGAA 3 cut(s) 796, 1140, 1266
TspGWI ACGGA 4 cut(s) 279, 399, 519, 1346
TspMI CCCGGG 1 cut(s) 556
TspRI CASTG 2 cut(s) 658, 1006
VpaK11BI GGWCC 3 cut(s) 266, 686, 1246
XapI RAATTY 5 cut(s) 215, 236, 282, 421, 656
XceI RCATGY 3 cut(s) 303, 606, 1486
XmaI CCCGGG 1 cut(s) 556
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.