Rroxscaffold_2G00142770

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004990
Physical Location & Seq
Reverse (-)
80851471 .. 80855806
4336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00142770.1

Sequence Viewer

Length: 1404 bp
ATGAAGCTAGCTCCAACGACGACGCCGTTTTTTCTCTCCGTCTTCTCAATCATCATACTCTGGACGACGTCGTTTGCGTTCACGCCTCGGACTTTACTTCTTCAGCTCCAGAGCTCGGCCGTGAGCACCAATTACTTGACCGATAAGGAGCTCTGGTTCACTCAGACTCTCGATCACTTCTCGGCGTACGATCACCGCAAGTTCGAGCAGCGATATTATGAGCTCCTTGACTTCTTCAGACCATCCGACGGTCCGATTTTCCTCAAAATCTGTGGAGAATCGGCCTGCAAAGGAATCTCCAACGATTACTTAACTGTTTTGGCTAAGAAGTTTGGCGCGGCTGTGGTCTCGCTCGAGCATCGTTACTATGGTAAGAGCTCTCCGTTTAAATCACACTCGACTGAGAATTTGAAGTACTTATCATCGAAGCAAGCGCTTTTCGATTTGGCTGCTTTTCGTCAGTATTATCAGGCAAGAGCTCTTAGTGCATGGTTTAGACTCAAGTTTCCTCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTTGAAGCTGTGTTTAACTACACTGAATTTGACCAGCAGATTGGTGAGTCTGCCGGTCCAGAATGTAAAGCTGCATTGCAAGAAACTAATCGTCTTGTTGAACAAAGGCTTGAAGCTAATGGAAAAGCAGTGCGGGGTTTGTTTGGTGCAACCCAGCTTGATATTGATGGTGATTTTATGTATTTTCTGGCGGATGCTGCTGTTATTGCGTTTCAATATGGAAATCCCGACAGACTGTGCTCCCCTCTTGTTCAAGCAAAGACTAATGGAGAGGATTTGGTTGAGGCATATGCCAAATACGTTAAAGAGTATTACCTTGGAAGTTTTGGTGTTGATGTCGAAACATACAATCAGAAACACTTGAAGAACACTGCTGTTACCGAGGGGAGTTCTGATCGTTTGTGGTGGTTCCAAGTTTGTACCGAAGTTGCCTATTTTCAGGTGGCACCTGCAAATGATAGCATCCGGTCTTCAAAAGTTGACACAAAATACCATTTGGACCTTTGCAAGAATGTCTTTGGAGAGGGCATCTATCCAGAGGTTGATGCAACAAATTTATATTATGGAGGCAAAAGAATTGCAGGTTCAAAAATAGTTTTCACAAATGGCTCACAGGATCCCTGGCGTCATGCATCCAAACAGACTTCATCTCCAGATATGCCTTCATACATCATCTCTTGCCATAATTGTGGCCATGGAACTGATTTGAGAGGATGTCCACAGGTTCCTTTAAGCCCCGAAGGTAATGCCAAGAACTGCAGCAACCCTGATGCAGTCCTCAAAGTTAGACAACAAATTGTAGAACACATAGACCTGTGGCTGTCTGAATGCCAAGAGACTGGTAGGAACTACATGTAA

Protein Analysis

467

Amino Acids

52.56

Weight (kDa)

6.28

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 55 - 418 1.1e-62 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1003
AatII GACGTC 1 cut(s) 71
Acc36I ACCTGC 2 cut(s) 1003, 1118
AccB1I GGYRCC 1 cut(s) 991
AccII CGCG 1 cut(s) 338
AciI CCGC 4 cut(s) 196, 338, 679, 737
AclWI GGATC 2 cut(s) 1157, 1170
AcoI YGGCCR 2 cut(s) 117, 1237
AcsI RAATTY 3 cut(s) 406, 572, 1099
AcuI CTGAAG 2 cut(s) 86, 220
AcyI GRCGYC 3 cut(s) 23, 68, 1171
AfaI GTAC 3 cut(s) 188, 416, 967
AfeI AGCGCT 1 cut(s) 435
AfiI CCNNNNNNNGG 2 cut(s) 115, 248
AflIII ACRYGT 2 cut(s) 518, 1398
AgsI TTSAA 9 cut(s) 412, 551, 647, 659, 761, 800, 910, 1020, 1134
AjnI CCWGG 1 cut(s) 1166
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
Alw21I GWGCWC 7 cut(s) 116, 128, 153, 225, 380, 481, 788
Alw26I GTCTC 2 cut(s) 352, 1376
AlwI GGATC 2 cut(s) 1157, 1170
Ama87I CYCGRG 1 cut(s) 353
Aor51HI AGCGCT 1 cut(s) 435
AoxI GGCC 3 cut(s) 117, 282, 1237
ApeKI GCWGC 5 cut(s) 208, 449, 617, 743, 1305
ApoI RAATTY 3 cut(s) 406, 572, 1099
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AspLEI GCGC 2 cut(s) 338, 436
AspS9I GGNCC 3 cut(s) 251, 602, 1045
AsuHPI GGTGA 3 cut(s) 185, 602, 728
AsuNHI GCTAGC 1 cut(s) 7
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 3 cut(s) 251, 602, 1045
BalI TGGCCA 1 cut(s) 1239
BamHI GGATCC 1 cut(s) 1162
BanI GGYRCC 1 cut(s) 991
BanII GRGCYC 5 cut(s) 116, 153, 225, 380, 481
BbsI GAAGAC 2 cut(s) 34, 1008
Bbv12I GWGCWC 7 cut(s) 116, 128, 153, 225, 380, 481, 788
BbvI GCAGC 5 cut(s) 220, 436, 604, 730, 1317
BccI CCATC 2 cut(s) 250, 707
BceAI ACGGC 2 cut(s) 10, 104
BcgI CGANNNNNNTGC 4 cut(s) 431, 465, 1274, 1308
BciT130I CCWGG 1 cut(s) 1168
BcoDI GTCTC 2 cut(s) 352, 1376
BfaI CTAG 1 cut(s) 8
BfmI CTRYAG 2 cut(s) 540, 1303
BfoI RGCGCY 1 cut(s) 437
BfuAI ACCTGC 2 cut(s) 1003, 1118
BisI GCNGC 6 cut(s) 209, 339, 450, 618, 744, 1306
BlsI GCNGC 6 cut(s) 210, 340, 451, 619, 745, 1307
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 1 cut(s) 1168
Bme18I GGWCC 3 cut(s) 251, 602, 1045
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 3 cut(s) 251, 602, 1045
BmiI GGNNCC 4 cut(s) 956, 993, 1164, 1272
BmrFI CCNGG 1 cut(s) 1168
BmsI GCATC 7 cut(s) 367, 730, 1017, 1081, 1083, 1187, 1306
BmtI GCTAGC 1 cut(s) 11
BpiI GAAGAC 2 cut(s) 34, 1008
BpmI CTGGAG 2 cut(s) 92, 1182
BpuEI CTTGAG 1 cut(s) 485
BsaHI GRCGYC 3 cut(s) 23, 68, 1171
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 5 cut(s) 86, 862, 927, 1166, 1240
BsaWI WCCGGW 1 cut(s) 1011
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 1180, 1210
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 248
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 1 cut(s) 1390
Bse3DI GCAATG 1 cut(s) 620
BseBI CCWGG 1 cut(s) 1168
BseDI CCNNGG 5 cut(s) 86, 862, 927, 1166, 1240
BseGI GGATG 5 cut(s) 242, 745, 1008, 1178, 1265
BseLI CCNNNNNNNGG 2 cut(s) 115, 248
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 2 cut(s) 176, 393
BseNI ACTGG 1 cut(s) 1390
BseX3I CGGCCG 1 cut(s) 117
BseXI GCAGC 5 cut(s) 220, 436, 604, 730, 1317
BseYI CCCAGC 1 cut(s) 699
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 119, 284, 1239
BshNI GGYRCC 1 cut(s) 991
BsiEI CGRYCG 1 cut(s) 120
BsiHKAI GWGCWC 7 cut(s) 116, 128, 153, 225, 380, 481, 788
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 600, 1012
BsiWI CGTACG 1 cut(s) 186
BslI CCNNNNNNNGG 2 cut(s) 115, 248
BsmAI GTCTC 2 cut(s) 352, 1376
BsmI GAATGC 1 cut(s) 1379
BsnI GGCC 3 cut(s) 119, 284, 1239
Bso31I GGTCTC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 7 cut(s) 116, 128, 153, 225, 380, 481, 788
Bsp143I GATC 4 cut(s) 172, 190, 940, 1162
Bsp19I CCATGG 1 cut(s) 1240
BspACI CCGC 4 cut(s) 196, 338, 679, 737
BspANI GGCC 3 cut(s) 119, 284, 1239
BspCNI CTCAG 2 cut(s) 175, 394
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 4 cut(s) 956, 993, 1164, 1272
BspMAI CTGCAG 2 cut(s) 544, 1307
BspMI ACCTGC 2 cut(s) 1003, 1118
BspOI GCTAGC 1 cut(s) 11
BspPI GGATC 2 cut(s) 1157, 1170
BspT107I GGYRCC 1 cut(s) 991
BspTNI GGTCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 1 cut(s) 1390
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 5 cut(s) 86, 862, 927, 1166, 1240
BssMI GATC 4 cut(s) 172, 190, 940, 1162
BssNI GRCGYC 3 cut(s) 23, 68, 1171
BssT1I CCWWGG 2 cut(s) 862, 1240
Bst2UI CCWGG 1 cut(s) 1168
Bst4CI ACNGT 3 cut(s) 251, 316, 783
BstACI GRCGYC 3 cut(s) 23, 68, 1171
BstAPI GCANNNNNTGC 1 cut(s) 539
BstC8I GCNNGC 3 cut(s) 9, 286, 432
BstDEI CTNAG 4 cut(s) 162, 324, 402, 482
BstDSI CCRYGG 1 cut(s) 1240
BstF5I GGATG 5 cut(s) 242, 745, 1008, 1178, 1265
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 2 cut(s) 338, 436
BstKTI GATC 4 cut(s) 175, 193, 943, 1165
BstMAI GTCTC 2 cut(s) 352, 1376
BstMBI GATC 4 cut(s) 172, 190, 940, 1162
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 4 cut(s) 485, 539, 743, 752
BstNI CCWGG 1 cut(s) 1168
BstNSI RCATGY 2 cut(s) 522, 1402
BstSCI CCNGG 1 cut(s) 1166
BstSFI CTRYAG 2 cut(s) 540, 1303
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 5 cut(s) 220, 436, 604, 730, 1317
BstV2I GAAGAC 2 cut(s) 34, 1008
BstX2I RGATCY 1 cut(s) 1162
BstXI CCANNNNNNTGG 3 cut(s) 587, 1235, 1385
BstYI RGATCY 1 cut(s) 1162
BstZI CGGCCG 1 cut(s) 117
BsuRI GGCC 3 cut(s) 119, 284, 1239
BtgI CCRYGG 1 cut(s) 1240
BtsCI GGATG 5 cut(s) 242, 745, 1008, 1178, 1265
BtsI GCAGTG 2 cut(s) 681, 915
BtsIMutI CAGTG 3 cut(s) 567, 681, 915
BveI ACCTGC 2 cut(s) 1003, 1118
Cac8I GCNNGC 3 cut(s) 9, 286, 432
CfoI GCGC 2 cut(s) 338, 436
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 3 cut(s) 251, 602, 1045
CpoI CGGWCCG 1 cut(s) 251
CseI GACGC 2 cut(s) 31, 1160
Csp6I GTAC 3 cut(s) 187, 415, 966
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CspI CGGWCCG 1 cut(s) 251
CviAII CATG 5 cut(s) 489, 519, 1175, 1241, 1399
CviQI GTAC 3 cut(s) 187, 415, 966
DdeI CTNAG 4 cut(s) 162, 324, 402, 482
DpnI GATC 4 cut(s) 174, 192, 942, 1164
DpnII GATC 4 cut(s) 172, 190, 940, 1162
DraI TTTAAA 1 cut(s) 388
EaeI YGGCCR 2 cut(s) 117, 1237
EagI CGGCCG 1 cut(s) 117
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 5 cut(s) 114, 151, 223, 378, 479
EclXI CGGCCG 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 862, 1240
Eco24I GRGCYC 5 cut(s) 116, 153, 225, 380, 481
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 3 cut(s) 251, 602, 1045
Eco47III AGCGCT 1 cut(s) 435
Eco52I CGGCCG 1 cut(s) 117
Eco53kI GAGCTC 5 cut(s) 114, 151, 223, 378, 479
Eco57I CTGAAG 2 cut(s) 86, 220
Eco88I CYCGRG 1 cut(s) 353
EcoICRI GAGCTC 5 cut(s) 114, 151, 223, 378, 479
EcoRII CCWGG 1 cut(s) 1166
EcoT14I CCWWGG 2 cut(s) 862, 1240
EcoT22I ATGCAT 1 cut(s) 1180
EcoT38I GRGCYC 5 cut(s) 116, 153, 225, 380, 481
ErhI CCWWGG 2 cut(s) 862, 1240
FaeI CATG 5 cut(s) 492, 522, 1178, 1244, 1402
FalI AAGNNNNNCTT 2 cut(s) 1046, 1078
FatI CATG 5 cut(s) 488, 518, 1174, 1240, 1398
FauI CCCGC 1 cut(s) 672
FauNDI CATATG 1 cut(s) 835
Fnu4HI GCNGC 6 cut(s) 209, 339, 450, 618, 744, 1306
FokI GGATG 5 cut(s) 229, 752, 995, 1165, 1272
FriOI GRGCYC 5 cut(s) 116, 153, 225, 380, 481
Fsp4HI GCNGC 6 cut(s) 209, 339, 450, 618, 744, 1306
FspBI CTAG 1 cut(s) 8
GlaI GCGC 2 cut(s) 337, 435
GluI GCNGC 6 cut(s) 209, 339, 450, 618, 744, 1306
GsaI CCCAGC 1 cut(s) 703
GsuI CTGGAG 2 cut(s) 92, 1182
HaeII RGCGCY 1 cut(s) 437
HaeIII GGCC 3 cut(s) 119, 284, 1239
HapII CCGG 2 cut(s) 600, 1012
HgaI GACGC 2 cut(s) 31, 1160
HhaI GCGC 2 cut(s) 338, 436
Hin1I GRCGYC 3 cut(s) 23, 68, 1171
Hin1II CATG 5 cut(s) 492, 522, 1178, 1244, 1402
Hin6I GCGC 2 cut(s) 336, 434
HinP1I GCGC 2 cut(s) 336, 434
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HinfI GANTC 5 cut(s) 166, 278, 294, 498, 593
HpaII CCGG 2 cut(s) 600, 1012
HphI GGTGA 3 cut(s) 185, 602, 728
Hpy166II GTNNAC 4 cut(s) 81, 159, 1027, 1265
Hpy188I TCNGA 8 cut(s) 90, 165, 239, 247, 255, 900, 940, 1372
Hpy188III TCNNGA 7 cut(s) 61, 109, 170, 605, 773, 1082, 1199
Hpy8I GTNNAC 4 cut(s) 81, 159, 1027, 1265
Hpy99I CGWCG 5 cut(s) 22, 25, 70, 73, 251
HpyAV CCTTC 2 cut(s) 1218, 1280
HpyCH4III ACNGT 3 cut(s) 251, 316, 783
HpyCH4IV ACGT 2 cut(s) 68, 846
HpyF10VI GCNNNNNNNGC 4 cut(s) 485, 539, 743, 752
HpyF3I CTNAG 4 cut(s) 162, 324, 402, 482
HpySE526I ACGT 2 cut(s) 68, 846
Hsp92I GRCGYC 3 cut(s) 23, 68, 1171
Hsp92II CATG 5 cut(s) 492, 522, 1178, 1244, 1402
HspAI GCGC 2 cut(s) 336, 434
Kzo9I GATC 4 cut(s) 172, 190, 940, 1162
LmnI GCTCC 5 cut(s) 16, 111, 148, 228, 791
Lsp1109I GCAGC 5 cut(s) 220, 436, 604, 730, 1317
LweI GCATC 7 cut(s) 367, 730, 1017, 1081, 1083, 1187, 1306
MaeI CTAG 1 cut(s) 8
MaeII ACGT 2 cut(s) 68, 846
MaeIII GTNAC 2 cut(s) 362, 922
MalI GATC 4 cut(s) 174, 192, 942, 1164
MboI GATC 4 cut(s) 172, 190, 940, 1162
MboII GAAGA 5 cut(s) 34, 92, 226, 922, 1008
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 7 cut(s) 116, 128, 153, 225, 380, 481, 788
MlsI TGGCCA 1 cut(s) 1239
MluCI AATT 7 cut(s) 130, 406, 572, 1099, 1122, 1231, 1341
MluNI TGGCCA 1 cut(s) 1239
MlyI GAGTC 3 cut(s) 160, 492, 602
MmeI TCCRAC 3 cut(s) 38, 270, 324
Mox20I TGGCCA 1 cut(s) 1239
Mph1103I ATGCAT 1 cut(s) 1180
MscI TGGCCA 1 cut(s) 1239
MseI TTAA 5 cut(s) 311, 387, 561, 849, 1277
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 1239
MspI CCGG 2 cut(s) 600, 1012
MspR9I CCNGG 1 cut(s) 1168
Mva1269I GAATGC 1 cut(s) 1379
MvaI CCWGG 1 cut(s) 1168
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 485, 539, 743, 752
NcoI CCATGG 1 cut(s) 1240
NdeI CATATG 1 cut(s) 835
NdeII GATC 4 cut(s) 172, 190, 940, 1162
NheI GCTAGC 1 cut(s) 7
NlaIII CATG 5 cut(s) 492, 522, 1178, 1244, 1402
NlaIV GGNNCC 4 cut(s) 956, 993, 1164, 1272
NmeAIII GCCGAG 2 cut(s) 95, 161
NsiI ATGCAT 1 cut(s) 1180
NspI RCATGY 2 cut(s) 522, 1402
PaeR7I CTCGAG 1 cut(s) 353
PaqCI CACCTGC 1 cut(s) 1003
PciI ACATGT 2 cut(s) 518, 1398
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 74
PctI GAATGC 1 cut(s) 1379
PfeI GAWTC 2 cut(s) 278, 294
Pfl23II CGTACG 1 cut(s) 186
PflFI GACNNNGTC 1 cut(s) 67
PkrI GCNGC 6 cut(s) 210, 340, 451, 619, 745, 1307
PleI GAGTC 3 cut(s) 160, 492, 601
PpsI GAGTC 3 cut(s) 160, 492, 601
PscI ACATGT 2 cut(s) 518, 1398
Psp124BI GAGCTC 5 cut(s) 116, 153, 225, 380, 481
Psp6I CCWGG 1 cut(s) 1166
PspFI CCCAGC 1 cut(s) 699
PspGI CCWGG 1 cut(s) 1166
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 4 cut(s) 956, 993, 1164, 1272
PspPI GGNCC 3 cut(s) 251, 602, 1045
PspXI VCTCGAGB 1 cut(s) 353
PstI CTGCAG 2 cut(s) 544, 1307
PsuI RGATCY 1 cut(s) 1162
PsyI GACNNNGTC 1 cut(s) 67
RsaI GTAC 3 cut(s) 188, 416, 967
RsaNI GTAC 3 cut(s) 187, 415, 966
RseI CAYNNNNRTG 1 cut(s) 1233
Rsr2I CGGWCCG 1 cut(s) 251
RsrII CGGWCCG 1 cut(s) 251
SacI GAGCTC 5 cut(s) 116, 153, 225, 380, 481
SaqAI TTAA 5 cut(s) 311, 387, 561, 849, 1277
SatI GCNGC 6 cut(s) 209, 339, 450, 618, 744, 1306
Sau3AI GATC 4 cut(s) 172, 190, 940, 1162
Sau96I GGNCC 3 cut(s) 251, 602, 1045
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 3 cut(s) 160, 492, 602
ScrFI CCNGG 1 cut(s) 1168
SduI GDGCHC 7 cut(s) 116, 128, 153, 225, 380, 481, 788
SfaNI GCATC 7 cut(s) 367, 730, 1017, 1081, 1083, 1187, 1306
SfcI CTRYAG 2 cut(s) 540, 1303
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 3 cut(s) 251, 602, 1045
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 2 cut(s) 353, 500
SmoI CTYRAG 2 cut(s) 353, 500
Sse9I AATT 7 cut(s) 130, 406, 572, 1099, 1122, 1231, 1341
SsiI CCGC 4 cut(s) 196, 338, 679, 737
SspMI CTAG 1 cut(s) 8
SstI GAGCTC 5 cut(s) 116, 153, 225, 380, 481
StyD4I CCNGG 1 cut(s) 1166
StyI CCWWGG 2 cut(s) 862, 1240
TaaI ACNGT 3 cut(s) 251, 316, 783
TaiI ACGT 2 cut(s) 71, 849
TaqI TCGA 7 cut(s) 171, 204, 354, 398, 425, 441, 885
TaqII GACCGA 1 cut(s) 155
TasI AATT 7 cut(s) 130, 406, 572, 1099, 1122, 1231, 1341
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 2 cut(s) 278, 294
Tru1I TTAA 5 cut(s) 311, 387, 561, 849, 1277
Tru9I TTAA 5 cut(s) 311, 387, 561, 849, 1277
TscAI CASTG 3 cut(s) 574, 681, 922
TseI GCWGC 5 cut(s) 208, 449, 617, 743, 1305
TspDTI ATGAA 3 cut(s) 17, 1182, 1200
TspGWI ACGGA 2 cut(s) 28, 372
TspRI CASTG 3 cut(s) 574, 681, 922
Tth111I GACNNNGTC 1 cut(s) 67
VpaK11BI GGWCC 3 cut(s) 251, 602, 1045
XapI RAATTY 3 cut(s) 406, 572, 1099
XceI RCATGY 2 cut(s) 522, 1402
XhoI CTCGAG 1 cut(s) 353
XspI CTAG 1 cut(s) 8
ZraI GACGTC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.