Rh2DG137300

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004990
Physical Location & Seq
Forward (+)
11506738 .. 11511802
5065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG137300.1

Sequence Viewer

Length: 1317 bp
ATGAAGCTAACTCCGATGACGACGCCGTTTTTTCTCTCCGTCTTCTCAATCATCATACTCTGGACGACGTCGTTTGCGTTCACGCCTCGGACTTTTCTTCAGCTCCAGAGCTCCGCCGTGAGCACCAATTACTTGACCGATAAGGAGCTCTGGTTCACTCAGACTCTCGATCACTTCTCGGCGTACGATCACCGCAAGTTCGAGCAGCGATATTATGAGCTCCTCGACTTTTTCAGACCATCGGACGGTCCGATTTTCCTCAGAATCTGTGGAGAATCGGCCTGCAAAGGAATCTCCAACGATTACTTAACTGTTTTGGCTAAGAAGTTTGGCGCGGCTGTGGTCTCGCTCGAGCATCGTTACTATGGTAAGAGCTCTCCGTTTAAATCACACTCGACTGAGAATTTGAAGTACTTATCATCGAAGCAAGCGCTTTTCGATTTGGCTGCTTTTCGTCAGTATTATCAGGAGTCGTTGAATTTGAAGCTGAATAAGACTAAGGGTGAGAATTCGTGGTTTGTATTCGGTGTGTCGTATCCAGGAGCTCTTAGTGCATGGTTTAGACTCAAGTTTCCTCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTTGAAGCTGTGTTTAACTACACTGAATTTGACCAGCAGATTGGTGAGTCTGCCGGTCCAGAATGTAAAGCTGCATTGCAAGAAACTAATCGTCTTGTTGAACAAAGGCTTGCACCTAATGGAAAAGCAGTGCGGGGTTTGTTTGGTGCAGCCCAGCTTGATATTGATGGTGATTTTATGTATTTTCTGGCGGATGCTGCTGTTATTGCGTTTCAATATGGAAATCCCGACAGACTGTGCTCCCCTCTTGTTCAAGCAAAGACTAATGGAGAGGATTTGGTTGAGGCATATGCCAAATATGTTAAAGAGTATTACCTTGGAAGTTTTGGTGTTGATGTCGAAACATACGATCAGAAACACTTGAAGAACACTGCTGTTACCGAGGGGAGTTCTGATCGTTTGTGGTGGTTCCAAGTTTGTACCGAAGTTGCCTATTTCCAGGTGGCACCTGCAAATGATAGCATCCGGTCTTCAAAAGTTGACACAAAATACCATTTGGACCTTTGCAAGAATGTCTTTGGAGAGGGCATCTATCCAGAGGTTGATGCGACAAATTTATATTATGGAGGCAAAAGAATAGCAGGTCAAAACATAAACCTTTCTTCCTATCCATGTGTTTCTCTTCCCTATAAGTATTTGTGCCATTATTACTATTATACTATCTTGGCTATGGATGGTCAGGTAACTTGCCCTATTTGCATGTAA

Protein Analysis

438

Amino Acids

49.64

Weight (kDa)

6.27

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 54 - 404 1.3e-66 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1069
AatII GACGTC 1 cut(s) 71
Acc36I ACCTGC 2 cut(s) 1069, 1184
AccB1I GGYRCC 1 cut(s) 1057
AccII CGCG 1 cut(s) 335
AciI CCGC 5 cut(s) 114, 193, 335, 745, 803
AcsI RAATTY 5 cut(s) 403, 478, 508, 638, 1165
AcuI CTGAAG 1 cut(s) 83
AcyI GRCGYC 2 cut(s) 23, 68
AfaI GTAC 3 cut(s) 185, 413, 1033
AfeI AGCGCT 1 cut(s) 432
AfiI CCNNNNNNNGG 1 cut(s) 245
AflIII ACRYGT 1 cut(s) 584
AgsI TTSAA 9 cut(s) 409, 478, 484, 617, 713, 827, 866, 976, 1086
AjnI CCWGG 2 cut(s) 538, 1050
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
Alw21I GWGCWC 7 cut(s) 113, 125, 150, 222, 377, 547, 854
Alw26I GTCTC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 350
Aor51HI AGCGCT 1 cut(s) 432
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 5 cut(s) 205, 446, 683, 761, 809
ApoI RAATTY 5 cut(s) 403, 478, 508, 638, 1165
ArsI GACNNNNNNTTYG 2 cut(s) 388, 420
AspLEI GCGC 2 cut(s) 335, 433
AspS9I GGNCC 3 cut(s) 248, 668, 1111
AsuHPI GGTGA 4 cut(s) 182, 515, 668, 794
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 248, 668, 1111
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 5 cut(s) 113, 150, 222, 377, 547
BbsI GAAGAC 2 cut(s) 34, 1074
Bbv12I GWGCWC 7 cut(s) 113, 125, 150, 222, 377, 547, 854
BbvI GCAGC 5 cut(s) 217, 433, 670, 773, 796
BccI CCATC 3 cut(s) 247, 773, 1280
BceAI ACGGC 2 cut(s) 10, 101
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 2 cut(s) 540, 1052
BciVI GTATCC 1 cut(s) 546
BcoDI GTCTC 1 cut(s) 349
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 434
BfuAI ACCTGC 2 cut(s) 1069, 1184
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 6 cut(s) 206, 336, 447, 684, 762, 810
BlsI GCNGC 6 cut(s) 207, 337, 448, 685, 763, 811
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 2 cut(s) 540, 1052
Bme18I GGWCC 3 cut(s) 248, 668, 1111
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 3 cut(s) 248, 668, 1111
BmiI GGNNCC 2 cut(s) 1022, 1059
BmrFI CCNGG 2 cut(s) 540, 1052
BmsI GCATC 5 cut(s) 364, 796, 1083, 1147, 1149
BpiI GAAGAC 2 cut(s) 34, 1074
BpmI CTGGAG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 551
BsaHI GRCGYC 2 cut(s) 23, 68
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 3 cut(s) 86, 928, 993
BsaWI WCCGGW 1 cut(s) 1077
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 665
Bse3DI GCAATG 1 cut(s) 686
BseBI CCWGG 2 cut(s) 540, 1052
BseDI CCNNGG 3 cut(s) 86, 928, 993
BseGI GGATG 3 cut(s) 811, 1074, 1291
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 686
BseMII CTCAG 3 cut(s) 173, 274, 390
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 5 cut(s) 217, 433, 670, 773, 796
BseYI CCCAGC 1 cut(s) 765
BsgI GTGCAG 1 cut(s) 780
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 1057
BsiHKAI GWGCWC 7 cut(s) 113, 125, 150, 222, 377, 547, 854
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 2 cut(s) 666, 1078
BsiWI CGTACG 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 349
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 7 cut(s) 113, 125, 150, 222, 377, 547, 854
Bsp143I GATC 4 cut(s) 169, 187, 961, 1006
BspACI CCGC 5 cut(s) 114, 193, 335, 745, 803
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 172, 273, 391
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 2 cut(s) 1022, 1059
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 2 cut(s) 1069, 1184
BspT107I GGYRCC 1 cut(s) 1057
BspTNI GGTCTC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 665
BssAI RCCGGY 1 cut(s) 665
BssECI CCNNGG 3 cut(s) 86, 928, 993
BssMI GATC 4 cut(s) 169, 187, 961, 1006
BssNI GRCGYC 2 cut(s) 23, 68
BssT1I CCWWGG 1 cut(s) 928
Bst2UI CCWGG 2 cut(s) 540, 1052
Bst4CI ACNGT 3 cut(s) 248, 313, 849
Bst6I CTCTTC 1 cut(s) 1239
BstACI GRCGYC 2 cut(s) 23, 68
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 3 cut(s) 283, 429, 723
BstDEI CTNAG 6 cut(s) 159, 260, 321, 399, 498, 548
BstF5I GGATG 3 cut(s) 811, 1074, 1291
BstFNI CGCG 1 cut(s) 335
BstH2I RGCGCY 1 cut(s) 434
BstHHI GCGC 2 cut(s) 335, 433
BstKTI GATC 4 cut(s) 172, 190, 964, 1009
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 4 cut(s) 169, 187, 961, 1006
BstMWI GCNNNNNNNGC 5 cut(s) 551, 605, 809, 818, 1308
BstNI CCWGG 2 cut(s) 540, 1052
BstNSI RCATGY 2 cut(s) 588, 1315
BstSCI CCNGG 2 cut(s) 538, 1050
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 5 cut(s) 217, 433, 670, 773, 796
BstV2I GAAGAC 2 cut(s) 34, 1074
BstXI CCANNNNNNTGG 1 cut(s) 653
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 1 cut(s) 281
BtsCI GGATG 3 cut(s) 811, 1074, 1291
BtsI GCAGTG 2 cut(s) 747, 981
BtsIMutI CAGTG 3 cut(s) 633, 747, 981
BveI ACCTGC 2 cut(s) 1069, 1184
Cac8I GCNNGC 3 cut(s) 283, 429, 723
CaiI CAGNNNCTG 1 cut(s) 267
CfoI GCGC 2 cut(s) 335, 433
Cfr10I RCCGGY 1 cut(s) 665
Cfr13I GGNCC 3 cut(s) 248, 668, 1111
CpoI CGGWCCG 1 cut(s) 248
CseI GACGC 1 cut(s) 31
Csp6I GTAC 3 cut(s) 184, 412, 1032
CspI CGGWCCG 1 cut(s) 248
CviAII CATG 4 cut(s) 555, 585, 1224, 1312
CviQI GTAC 3 cut(s) 184, 412, 1032
DdeI CTNAG 6 cut(s) 159, 260, 321, 399, 498, 548
DpnI GATC 4 cut(s) 171, 189, 963, 1008
DpnII GATC 4 cut(s) 169, 187, 961, 1006
DraI TTTAAA 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 1239
EarI CTCTTC 1 cut(s) 1239
EciI GGCGGA 2 cut(s) 103, 818
Ecl136II GAGCTC 5 cut(s) 111, 148, 220, 375, 545
Eco130I CCWWGG 1 cut(s) 928
Eco24I GRGCYC 5 cut(s) 113, 150, 222, 377, 547
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 3 cut(s) 248, 668, 1111
Eco47III AGCGCT 1 cut(s) 432
Eco53kI GAGCTC 5 cut(s) 111, 148, 220, 375, 545
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 5 cut(s) 111, 148, 220, 375, 545
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 2 cut(s) 538, 1050
EcoT14I CCWWGG 1 cut(s) 928
EcoT38I GRGCYC 5 cut(s) 113, 150, 222, 377, 547
ErhI CCWWGG 1 cut(s) 928
FaeI CATG 4 cut(s) 558, 588, 1227, 1315
FalI AAGNNNNNCTT 2 cut(s) 1112, 1144
FatI CATG 4 cut(s) 554, 584, 1223, 1311
FauI CCCGC 1 cut(s) 738
FauNDI CATATG 1 cut(s) 901
Fnu4HI GCNGC 6 cut(s) 206, 336, 447, 684, 762, 810
FokI GGATG 3 cut(s) 818, 1061, 1298
FriOI GRGCYC 5 cut(s) 113, 150, 222, 377, 547
Fsp4HI GCNGC 6 cut(s) 206, 336, 447, 684, 762, 810
GlaI GCGC 2 cut(s) 334, 432
GluI GCNGC 6 cut(s) 206, 336, 447, 684, 762, 810
GsaI CCCAGC 1 cut(s) 769
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 434
HaeIII GGCC 1 cut(s) 281
HapII CCGG 2 cut(s) 666, 1078
HgaI GACGC 1 cut(s) 31
HhaI GCGC 2 cut(s) 335, 433
Hin1I GRCGYC 2 cut(s) 23, 68
Hin1II CATG 4 cut(s) 558, 588, 1227, 1315
Hin6I GCGC 2 cut(s) 333, 431
HinP1I GCGC 2 cut(s) 333, 431
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HinfI GANTC 7 cut(s) 163, 264, 275, 291, 470, 564, 659
HpaII CCGG 2 cut(s) 666, 1078
HphI GGTGA 4 cut(s) 182, 515, 668, 794
Hpy166II GTNNAC 3 cut(s) 81, 156, 1093
Hpy188I TCNGA 9 cut(s) 15, 90, 162, 236, 244, 252, 263, 966, 1006
Hpy188III TCNNGA 7 cut(s) 61, 106, 167, 467, 671, 839, 1148
Hpy8I GTNNAC 3 cut(s) 81, 156, 1093
Hpy99I CGWCG 3 cut(s) 25, 70, 73
HpyCH4III ACNGT 3 cut(s) 248, 313, 849
HpyCH4IV ACGT 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 5 cut(s) 551, 605, 809, 818, 1308
HpyF3I CTNAG 6 cut(s) 159, 260, 321, 399, 498, 548
HpySE526I ACGT 1 cut(s) 68
Hsp92I GRCGYC 2 cut(s) 23, 68
Hsp92II CATG 4 cut(s) 558, 588, 1227, 1315
HspAI GCGC 2 cut(s) 333, 431
Kzo9I GATC 4 cut(s) 169, 187, 961, 1006
LmnI GCTCC 6 cut(s) 108, 116, 145, 225, 542, 857
Lsp1109I GCAGC 5 cut(s) 217, 433, 670, 773, 796
LweI GCATC 5 cut(s) 364, 796, 1083, 1147, 1149
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 3 cut(s) 359, 988, 1294
MalI GATC 4 cut(s) 171, 189, 963, 1008
MboI GATC 4 cut(s) 169, 187, 961, 1006
MboII GAAGA 6 cut(s) 34, 89, 988, 1074, 1206, 1226
MhlI GDGCHC 7 cut(s) 113, 125, 150, 222, 377, 547, 854
MluCI AATT 6 cut(s) 127, 403, 478, 508, 638, 1165
MlyI GAGTC 4 cut(s) 157, 479, 558, 668
MmeI TCCRAC 1 cut(s) 321
MseI TTAA 4 cut(s) 308, 384, 627, 915
MspI CCGG 2 cut(s) 666, 1078
MspR9I CCNGG 2 cut(s) 540, 1052
MvaI CCWGG 2 cut(s) 540, 1052
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 5 cut(s) 551, 605, 809, 818, 1308
NdeI CATATG 1 cut(s) 901
NdeII GATC 4 cut(s) 169, 187, 961, 1006
NlaIII CATG 4 cut(s) 558, 588, 1227, 1315
NlaIV GGNNCC 2 cut(s) 1022, 1059
NmeAIII GCCGAG 1 cut(s) 158
NspI RCATGY 2 cut(s) 588, 1315
PaeR7I CTCGAG 1 cut(s) 350
PaqCI CACCTGC 1 cut(s) 1069
PciI ACATGT 1 cut(s) 584
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 248, 957
PfeI GAWTC 3 cut(s) 264, 275, 291
Pfl23II CGTACG 1 cut(s) 183
PflFI GACNNNGTC 1 cut(s) 67
PfoI TCCNGGA 1 cut(s) 538
PkrI GCNGC 6 cut(s) 207, 337, 448, 685, 763, 811
PleI GAGTC 4 cut(s) 157, 478, 558, 667
PpsI GAGTC 4 cut(s) 157, 478, 558, 667
PscI ACATGT 1 cut(s) 584
Psp124BI GAGCTC 5 cut(s) 113, 150, 222, 377, 547
Psp6I CCWGG 2 cut(s) 538, 1050
PspFI CCCAGC 1 cut(s) 765
PspGI CCWGG 2 cut(s) 538, 1050
PspLI CGTACG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 1022, 1059
PspPI GGNCC 3 cut(s) 248, 668, 1111
PspXI VCTCGAGB 1 cut(s) 350
PstI CTGCAG 1 cut(s) 610
PstNI CAGNNNCTG 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 67
RsaI GTAC 3 cut(s) 185, 413, 1033
RsaNI GTAC 3 cut(s) 184, 412, 1032
Rsr2I CGGWCCG 1 cut(s) 248
RsrII CGGWCCG 1 cut(s) 248
SacI GAGCTC 5 cut(s) 113, 150, 222, 377, 547
SaqAI TTAA 4 cut(s) 308, 384, 627, 915
SatI GCNGC 6 cut(s) 206, 336, 447, 684, 762, 810
Sau3AI GATC 4 cut(s) 169, 187, 961, 1006
Sau96I GGNCC 3 cut(s) 248, 668, 1111
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 4 cut(s) 157, 479, 558, 668
ScrFI CCNGG 2 cut(s) 540, 1052
SduI GDGCHC 7 cut(s) 113, 125, 150, 222, 377, 547, 854
SfaNI GCATC 5 cut(s) 364, 796, 1083, 1147, 1149
SfcI CTRYAG 1 cut(s) 606
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 3 cut(s) 248, 668, 1111
SlaI CTCGAG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 350, 566
SmoI CTYRAG 2 cut(s) 350, 566
Sse9I AATT 6 cut(s) 127, 403, 478, 508, 638, 1165
SsiI CCGC 5 cut(s) 114, 193, 335, 745, 803
SstI GAGCTC 5 cut(s) 113, 150, 222, 377, 547
StyD4I CCNGG 2 cut(s) 538, 1050
StyI CCWWGG 1 cut(s) 928
TaaI ACNGT 3 cut(s) 248, 313, 849
TaiI ACGT 1 cut(s) 71
TaqI TCGA 8 cut(s) 168, 201, 225, 351, 395, 422, 438, 951
TaqII GACCGA 1 cut(s) 152
TasI AATT 6 cut(s) 127, 403, 478, 508, 638, 1165
TatI WGTACW 1 cut(s) 411
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 3 cut(s) 264, 275, 291
Tru1I TTAA 4 cut(s) 308, 384, 627, 915
Tru9I TTAA 4 cut(s) 308, 384, 627, 915
TscAI CASTG 3 cut(s) 640, 747, 988
TseI GCWGC 5 cut(s) 205, 446, 683, 761, 809
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 28, 369
TspRI CASTG 3 cut(s) 640, 747, 988
Tth111I GACNNNGTC 1 cut(s) 67
VpaK11BI GGWCC 3 cut(s) 248, 668, 1111
XapI RAATTY 5 cut(s) 403, 478, 508, 638, 1165
XceI RCATGY 2 cut(s) 588, 1315
XhoI CTCGAG 1 cut(s) 350
ZraI GACGTC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.