MD02G1110800.v1.1

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004990
Physical Location & Seq
Forward (+)
9012320 .. 9015865
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1110800.v1.1.491

Sequence Viewer

Length: 1473 bp
ATGATGACGTCGTTTTCGTTCTCCTTGTCCTTCCTCTTCGCAATGATGATGTGGACGCCGCTCTCTTATGGCTTTGTCAATCCGCGAACACTTCTTCAGCTGGAGAGCTCGTCGGGAAGCGGCGGCGGAAAGTTATTGACGAAGGAGGAGCTGTGGTTCGATCAGACTCTTGACCACTTCTCGCCGTACGACCGCCGCAAATTCCCGCAGCGCTACTACGAATTTCTCGACTACTTCAGACCTTCCGACGGACCAATTTTCCTGAAAATTTGCGGAGAATCCGCATGCGGCGGCATTGCCAACGATTACCTGAGTGTGTTGGCTAAGAAGTTTGGGGCAGCTATCGTTTCGCCCGAGCATCGATACTACGGAAAAAGCTCTCCTTTCAAGACGCATACAACGCAGAATTTGAAGTACTTGTCATCCAAGCAAGCGCTTTTCGATCTGGCGGCTTTTCGTGAGTTTTATCAGAATGGGTTAAATGTGAAGCTGAATAGAAACAAGGGTGACAATCCGTGGTTCGTGTTTGGTGTTTCTTACCCGGGAGCTCTTAGTGCGTGGTTTCGGGTTAAGTTCCCGCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTTGAAGCTATTTATGACTTCACTGAATTTGACCAGCAGATTGGTGAGTCTGCAGGTCCAGAATGTAAAGCTGCACTTCAAGAAACTACTCATCTTGTTGAACAAAGTCTCACAACTAATGGACAAGCAATAAAGGCGTTGTTTGGTGCAAGCCAGCTTGATATTGATGGTGACTTCATGTATTTTCTGGCGGATGCTGCTGTTATAGCGTTTCAATATGGAAATCCCGATAAATTATGCTCACCTCTTGTTCAAGCAAAGACGAATGGAGAGGATTTGGTGGAAGCGTACGCCAAATACATTAAAGATTATTACCTTGGAACTTTTGGCATTGACGTTGAAACATATAACCAGAAACACTTGAAGAACACTGCTGTTACTGAGGGCAGTTCTGATCGGTTGTGGTGGTTCCAAGTTTGTACTGAAGTTGCATATTTTCAGGTGGCTCCTGCAAATGATAGCATCAGGTCTTCAAAAGTTGACACAAAATACCATGAAGACCTTTGCAAAAATGTTTTTGGAGACGGCATCTATCCTGACGTTCTTGCAACAAACTTATACTATGGAGGCACAAAAATTGCAGGTTCAAAAATTGTTTTTACAAATGGTTCACAGGACCCCTGGCGTCATGCATCCAAACAAACTTCATCTCCAGAAATGCCTTCGTACATCATCTCTTGCCATAACTGTGGCCATGGAACGGATTTGAGAGGATGCCCTCAGTCTCCTTTAACCCTTGAAGGCAATTCCCAGAACTGCAGCAACCCTGATGCAGTCAACAAAGTACGGCAACAAATTGTTGAACACATCGACTTGTGGCTGTCTGAATGCCAAGAAGCCGGTAGGAGTTACATGTAA

Protein Analysis

491

Amino Acids

54.81

Weight (kDa)

5.71

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 55 - 442 6.6e-76 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
Acc36I ACCTGC 2 cut(s) 659, 1187
AccBSI CCGCTC 1 cut(s) 61
AccII CGCG 1 cut(s) 85
AcoI YGGCCR 1 cut(s) 1306
AcsI RAATTY 5 cut(s) 200, 221, 267, 406, 641
AcuI CTGAAG 3 cut(s) 80, 220, 1059
AcyI GRCGYC 3 cut(s) 8, 56, 1240
AfaI GTAC 6 cut(s) 188, 416, 905, 1036, 1283, 1401
AfeI AGCGCT 2 cut(s) 212, 435
AfiI CCNNNNNNNGG 2 cut(s) 248, 1315
AflIII ACRYGT 2 cut(s) 587, 1467
AjnI CCWGG 1 cut(s) 1235
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
Alw21I GWGCWC 2 cut(s) 110, 550
Alw26I GTCTC 3 cut(s) 728, 1131, 1344
Ama87I CYCGRG 2 cut(s) 353, 541
Aor51HI AGCGCT 2 cut(s) 212, 435
AoxI GGCC 1 cut(s) 1306
ApeKI GCWGC 5 cut(s) 208, 338, 686, 812, 1374
ApoI RAATTY 5 cut(s) 200, 221, 267, 406, 641
ArsI GACNNNNNNTTYG 1 cut(s) 30
AspLEI GCGC 2 cut(s) 213, 436
AspS9I GGNCC 3 cut(s) 251, 671, 1231
AsuC2I CCSGG 2 cut(s) 542, 543
AsuHPI GGTGA 4 cut(s) 518, 671, 797, 849
AvaI CYCGRG 2 cut(s) 353, 541
AvaII GGWCC 3 cut(s) 251, 671, 1231
BalI TGGCCA 1 cut(s) 1308
BanII GRGCYC 2 cut(s) 110, 550
BbsI GAAGAC 2 cut(s) 1077, 1119
Bbv12I GWGCWC 2 cut(s) 110, 550
BbvI GCAGC 5 cut(s) 220, 350, 673, 799, 1386
BccI CCATC 1 cut(s) 776
BceAI ACGGC 3 cut(s) 169, 1156, 1418
BciT130I CCWGG 1 cut(s) 1237
BcnI CCSGG 2 cut(s) 542, 543
BcoDI GTCTC 3 cut(s) 728, 1131, 1344
BfmI CTRYAG 3 cut(s) 609, 666, 1372
BfoI RGCGCY 2 cut(s) 214, 437
BfuAI ACCTGC 2 cut(s) 659, 1187
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 542, 543, 1237
Bme18I GGWCC 3 cut(s) 251, 671, 1231
BmeT110I CYCGRG 2 cut(s) 353, 541
BmgT120I GGNCC 3 cut(s) 251, 671, 1231
BmiI GGNNCC 3 cut(s) 1025, 1062, 1233
BmrFI CCNGG 3 cut(s) 542, 543, 1237
BmsI GCATC 7 cut(s) 367, 799, 1086, 1152, 1256, 1319, 1375
BpiI GAAGAC 2 cut(s) 1077, 1119
BpmI CTGGAG 2 cut(s) 122, 1251
BpuMI CCSGG 2 cut(s) 542, 543
Bsa29I ATCGAT 1 cut(s) 361
BsaHI GRCGYC 3 cut(s) 8, 56, 1240
BsaJI CCNNGG 5 cut(s) 515, 541, 931, 1235, 1309
BsaXI ACNNNNNCTCC 8 cut(s) 95, 125, 137, 140, 167, 170, 1249, 1279
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1315
Bse118I RCCGGY 1 cut(s) 1454
Bse3DI GCAATG 2 cut(s) 48, 294
BseBI CCWGG 1 cut(s) 1237
BseCI ATCGAT 1 cut(s) 361
BseDI CCNNGG 5 cut(s) 515, 541, 931, 1235, 1309
BseGI GGATG 4 cut(s) 422, 814, 1247, 1334
BseLI CCNNNNNNNGG 2 cut(s) 248, 1315
BseMI GCAATG 2 cut(s) 48, 294
BseMII CTCAG 3 cut(s) 302, 987, 1349
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 5 cut(s) 220, 350, 673, 799, 1386
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 1 cut(s) 1308
BshVI ATCGAT 1 cut(s) 361
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 2 cut(s) 110, 550
BsiHKCI CYCGRG 2 cut(s) 353, 541
BsiSI CCGG 2 cut(s) 542, 1455
BsiWI CGTACG 2 cut(s) 186, 903
BslI CCNNNNNNNGG 2 cut(s) 248, 1315
BsmAI GTCTC 3 cut(s) 728, 1131, 1344
BsmBI CGTCTC 1 cut(s) 1131
BsmI GAATGC 1 cut(s) 1448
BsnI GGCC 1 cut(s) 1308
BsoBI CYCGRG 2 cut(s) 353, 541
Bsp1286I GDGCHC 2 cut(s) 110, 550
Bsp143I GATC 3 cut(s) 160, 442, 1009
Bsp19I CCATGG 1 cut(s) 1309
BspANI GGCC 1 cut(s) 1308
BspCNI CTCAG 3 cut(s) 303, 988, 1348
BspDI ATCGAT 1 cut(s) 361
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 1025, 1062, 1233
BspMAI CTGCAG 3 cut(s) 613, 670, 1376
BspMI ACCTGC 2 cut(s) 659, 1187
BsrBI CCGCTC 1 cut(s) 61
BsrDI GCAATG 2 cut(s) 48, 294
BsrFI RCCGGY 1 cut(s) 1454
BssAI RCCGGY 1 cut(s) 1454
BssECI CCNNGG 5 cut(s) 515, 541, 931, 1235, 1309
BssMI GATC 3 cut(s) 160, 442, 1009
BssNI GRCGYC 3 cut(s) 8, 56, 1240
BssT1I CCWWGG 2 cut(s) 931, 1309
Bst2UI CCWGG 1 cut(s) 1237
Bst4CI ACNGT 1 cut(s) 1304
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 3 cut(s) 8, 56, 1240
BstAPI GCANNNNNTGC 1 cut(s) 608
BstC8I GCNNGC 4 cut(s) 286, 432, 766, 770
BstDEI CTNAG 5 cut(s) 311, 324, 551, 996, 1335
BstDSI CCRYGG 2 cut(s) 515, 1309
BstF5I GGATG 4 cut(s) 422, 814, 1247, 1334
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 2 cut(s) 214, 437
BstHHI GCGC 2 cut(s) 213, 436
BstKTI GATC 3 cut(s) 163, 445, 1012
BstMAI GTCTC 3 cut(s) 728, 1131, 1344
BstMBI GATC 3 cut(s) 160, 442, 1009
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 6 cut(s) 400, 554, 608, 749, 812, 821
BstNI CCWGG 1 cut(s) 1237
BstNSI RCATGY 3 cut(s) 288, 591, 1471
BstSCI CCNGG 3 cut(s) 540, 541, 1235
BstSFI CTRYAG 3 cut(s) 609, 666, 1372
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 5 cut(s) 220, 350, 673, 799, 1386
BstV2I GAAGAC 2 cut(s) 1077, 1119
BstXI CCANNNNNNTGG 2 cut(s) 656, 1304
Bsu15I ATCGAT 1 cut(s) 361
BsuRI GGCC 1 cut(s) 1308
BsuTUI ATCGAT 1 cut(s) 361
BtgI CCRYGG 2 cut(s) 515, 1309
BtsCI GGATG 4 cut(s) 422, 814, 1247, 1334
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 2 cut(s) 636, 984
BveI ACCTGC 2 cut(s) 659, 1187
Cac8I GCNNGC 4 cut(s) 286, 432, 766, 770
CfoI GCGC 2 cut(s) 213, 436
Cfr10I RCCGGY 1 cut(s) 1454
Cfr13I GGNCC 3 cut(s) 251, 671, 1231
Cfr9I CCCGGG 1 cut(s) 541
ClaI ATCGAT 1 cut(s) 361
CseI GACGC 3 cut(s) 64, 400, 1229
Csp6I GTAC 6 cut(s) 187, 415, 904, 1035, 1282, 1400
CviAII CATG 7 cut(s) 285, 588, 793, 1109, 1244, 1310, 1468
CviQI GTAC 6 cut(s) 187, 415, 904, 1035, 1282, 1400
DdeI CTNAG 5 cut(s) 311, 324, 551, 996, 1335
DpnI GATC 3 cut(s) 162, 444, 1011
DpnII GATC 3 cut(s) 160, 442, 1009
EaeI YGGCCR 1 cut(s) 1306
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 2 cut(s) 141, 821
Ecl136II GAGCTC 2 cut(s) 108, 548
Eco130I CCWWGG 2 cut(s) 931, 1309
Eco24I GRGCYC 2 cut(s) 110, 550
Eco47I GGWCC 3 cut(s) 251, 671, 1231
Eco47III AGCGCT 2 cut(s) 212, 435
Eco53kI GAGCTC 2 cut(s) 108, 548
Eco57I CTGAAG 3 cut(s) 80, 220, 1059
Eco88I CYCGRG 2 cut(s) 353, 541
EcoICRI GAGCTC 2 cut(s) 108, 548
EcoO109I RGGNCCY 1 cut(s) 1231
EcoRII CCWGG 1 cut(s) 1235
EcoT14I CCWWGG 2 cut(s) 931, 1309
EcoT22I ATGCAT 1 cut(s) 1249
EcoT38I GRGCYC 2 cut(s) 110, 550
ErhI CCWWGG 2 cut(s) 931, 1309
Esp3I CGTCTC 1 cut(s) 1131
FaeI CATG 7 cut(s) 288, 591, 796, 1112, 1247, 1313, 1471
FalI AAGNNNNNCTT 4 cut(s) 367, 399, 675, 707
FatI CATG 7 cut(s) 284, 587, 792, 1108, 1243, 1309, 1467
FauI CCCGC 2 cut(s) 213, 585
FokI GGATG 4 cut(s) 409, 821, 1234, 1341
FriOI GRGCYC 2 cut(s) 110, 550
GlaI GCGC 2 cut(s) 212, 435
GsuI CTGGAG 2 cut(s) 122, 1251
HaeII RGCGCY 2 cut(s) 214, 437
HaeIII GGCC 1 cut(s) 1308
HapII CCGG 2 cut(s) 542, 1455
HgaI GACGC 3 cut(s) 64, 400, 1229
HhaI GCGC 2 cut(s) 213, 436
Hin1I GRCGYC 3 cut(s) 8, 56, 1240
Hin1II CATG 7 cut(s) 288, 591, 796, 1112, 1247, 1313, 1471
Hin6I GCGC 2 cut(s) 211, 434
HinP1I GCGC 2 cut(s) 211, 434
HincII GTYRAC 2 cut(s) 1096, 1393
HindII GTYRAC 2 cut(s) 1096, 1393
HinfI GANTC 3 cut(s) 166, 278, 662
HpaII CCGG 2 cut(s) 542, 1455
HphI GGTGA 4 cut(s) 518, 671, 797, 849
Hpy166II GTNNAC 4 cut(s) 54, 1096, 1226, 1393
Hpy188I TCNGA 6 cut(s) 165, 239, 247, 471, 1009, 1441
Hpy8I GTNNAC 4 cut(s) 54, 1096, 1226, 1393
Hpy99I CGWCG 3 cut(s) 13, 115, 251
HpyAV CCTTC 5 cut(s) 40, 136, 252, 1287, 1349
HpyCH4III ACNGT 1 cut(s) 1304
HpyCH4IV ACGT 3 cut(s) 8, 951, 1155
HpyF10VI GCNNNNNNNGC 6 cut(s) 400, 554, 608, 749, 812, 821
HpyF3I CTNAG 5 cut(s) 311, 324, 551, 996, 1335
HpySE526I ACGT 3 cut(s) 8, 951, 1155
Hsp92I GRCGYC 3 cut(s) 8, 56, 1240
Hsp92II CATG 7 cut(s) 288, 591, 796, 1112, 1247, 1313, 1471
HspAI GCGC 2 cut(s) 211, 434
Kzo9I GATC 3 cut(s) 160, 442, 1009
LmnI GCTCC 3 cut(s) 148, 545, 1066
Lsp1109I GCAGC 5 cut(s) 220, 350, 673, 799, 1386
LweI GCATC 7 cut(s) 367, 799, 1086, 1152, 1256, 1319, 1375
MaeII ACGT 3 cut(s) 8, 951, 1155
MaeIII GTNAC 4 cut(s) 506, 785, 991, 1463
MalI GATC 3 cut(s) 162, 444, 1011
MbiI CCGCTC 1 cut(s) 61
MboI GATC 3 cut(s) 160, 442, 1009
MboII GAAGA 5 cut(s) 28, 86, 991, 1077, 1124
MhlI GDGCHC 2 cut(s) 110, 550
MlsI TGGCCA 1 cut(s) 1308
MluNI TGGCCA 1 cut(s) 1308
MlyI GAGTC 2 cut(s) 160, 671
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 8 cut(s) 44, 139, 870, 880, 991, 1175, 1319, 1344
Mox20I TGGCCA 1 cut(s) 1308
Mph1103I ATGCAT 1 cut(s) 1249
MscI TGGCCA 1 cut(s) 1308
MseI TTAA 4 cut(s) 479, 570, 918, 1346
MslI CAYNNNNRTG 1 cut(s) 1302
Msp20I TGGCCA 1 cut(s) 1308
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 2 cut(s) 542, 1455
MspR9I CCNGG 3 cut(s) 542, 543, 1237
Mva1269I GAATGC 1 cut(s) 1448
MvaI CCWGG 1 cut(s) 1237
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 6 cut(s) 400, 554, 608, 749, 812, 821
NciI CCSGG 2 cut(s) 542, 543
NcoI CCATGG 1 cut(s) 1309
NdeII GATC 3 cut(s) 160, 442, 1009
NlaIII CATG 7 cut(s) 288, 591, 796, 1112, 1247, 1313, 1471
NlaIV GGNNCC 3 cut(s) 1025, 1062, 1233
NmuCI GTSAC 2 cut(s) 506, 785
NsiI ATGCAT 1 cut(s) 1249
NspI RCATGY 3 cut(s) 288, 591, 1471
PaeI GCATGC 1 cut(s) 288
PciI ACATGT 2 cut(s) 587, 1467
PcsI WCGNNNNNNNCGW 3 cut(s) 14, 225, 351
PctI GAATGC 1 cut(s) 1448
PfeI GAWTC 1 cut(s) 278
Pfl23II CGTACG 2 cut(s) 186, 903
PleI GAGTC 2 cut(s) 160, 670
PpsI GAGTC 2 cut(s) 160, 670
PpuMI RGGWCCY 1 cut(s) 1231
PscI ACATGT 2 cut(s) 587, 1467
Psp124BI GAGCTC 2 cut(s) 110, 550
Psp5II RGGWCCY 1 cut(s) 1231
Psp6I CCWGG 1 cut(s) 1235
PspGI CCWGG 1 cut(s) 1235
PspLI CGTACG 2 cut(s) 186, 903
PspN4I GGNNCC 3 cut(s) 1025, 1062, 1233
PspPI GGNCC 3 cut(s) 251, 671, 1231
PspPPI RGGWCCY 1 cut(s) 1231
PstI CTGCAG 3 cut(s) 613, 670, 1376
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 6 cut(s) 188, 416, 905, 1036, 1283, 1401
RsaNI GTAC 6 cut(s) 187, 415, 904, 1035, 1282, 1400
RseI CAYNNNNRTG 1 cut(s) 1302
SacI GAGCTC 2 cut(s) 110, 550
SaqAI TTAA 4 cut(s) 479, 570, 918, 1346
Sau3AI GATC 3 cut(s) 160, 442, 1009
Sau96I GGNCC 3 cut(s) 251, 671, 1231
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 2 cut(s) 160, 671
ScrFI CCNGG 3 cut(s) 542, 543, 1237
SduI GDGCHC 2 cut(s) 110, 550
SfaNI GCATC 7 cut(s) 367, 799, 1086, 1152, 1256, 1319, 1375
SfcI CTRYAG 3 cut(s) 609, 666, 1372
SinI GGWCC 3 cut(s) 251, 671, 1231
SmaI CCCGGG 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 1302
SphI GCATGC 1 cut(s) 288
SstI GAGCTC 2 cut(s) 110, 550
StyD4I CCNGG 3 cut(s) 540, 541, 1235
StyI CCWWGG 2 cut(s) 931, 1309
TaaI ACNGT 1 cut(s) 1304
TaiI ACGT 3 cut(s) 11, 954, 1158
TaqI TCGA 5 cut(s) 159, 228, 361, 441, 1425
TatI WGTACW 2 cut(s) 414, 1034
TauI GCSGC 7 cut(s) 61, 123, 126, 198, 291, 294, 452
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 4 cut(s) 479, 570, 918, 1346
Tru9I TTAA 4 cut(s) 479, 570, 918, 1346
TscAI CASTG 2 cut(s) 643, 991
TseFI GTSAC 2 cut(s) 506, 785
TseI GCWGC 5 cut(s) 208, 338, 686, 812, 1374
Tsp45I GTSAC 2 cut(s) 506, 785
TspDTI ATGAA 3 cut(s) 781, 1125, 1251
TspGWI ACGGA 4 cut(s) 264, 384, 504, 1331
TspMI CCCGGG 1 cut(s) 541
TspRI CASTG 2 cut(s) 643, 991
VpaK11BI GGWCC 3 cut(s) 251, 671, 1231
XapI RAATTY 5 cut(s) 200, 221, 267, 406, 641
XceI RCATGY 3 cut(s) 288, 591, 1471
XmaI CCCGGG 1 cut(s) 541
ZraI GACGTC 1 cut(s) 9
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.