AT2G19270

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016126
Physical Location & Seq
Reverse (-)
8360150 .. 8361951
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G19270.1

Sequence Viewer

Length: 1080 bp
ATGGACTCTCTGGTAGCGAGCTACGCTTCGTCGGACGAAGAAGAAGATCTTCAACCACGTCGAGAAGCTTTCACTGTAAAATCCTCAGAACCTTCCGCTTCGTCTCTATTCTCAGCTATTCCTCAACCTAAAGAACTCAAATCCTCCGAAGATGGTGATTTAGGCTCATCTTCCTCTCGAGGAAAATCTTCTTCTTTCCTCTCATCTCTCCCTCCTCCCAAATCCTCAATTTCACGGAAAAAAAATCCCAATCCGTCGTCAATTCCGAAACGTGTCGTCCAGATCAGGCTTCCAGTAAACCCTAGACCGAGCAATCTTGACGACGAAGACGACGAAGAAGAAGAGAAAGCTAGGAAGAAGCGTAAACAGATGGAATCTGCTTCCGCATCTCACGATTCATCAGTAAGATCGTTCTTATCCGCCATGCCTGCACCTAAAAGCTCACAAACACTAGGTGCTCTTCCTTCATTGGGATCAGGATCAGGTCGAAGATCGAATCTCGAAACAGAAACACCTTCAATTGCATTTCCTCAAACGGATTCAGGTATTAGTGATCAACAAAACCAGAGCTATGAATCGTTTAGTCATAGTAATAGCGAAACGGAACAAATCGTTGGAGTAGATAACTATTATGCTACTGGTTATGATGGTGGATACGAAACAATTCCTAGTGGTAGTAGTGTGGGATATGATGGTAGTAGCTATGGAGGTAATACTTGGAATGGTGGTGGGTTTGAGGCAATGACGGGATTGCCTGAAGCGGTTGTAGCTATGGATAGTGGTGCGAGGAGAGGAAGGAGAGGGAGGAATCAGATGCCAGAGATTGTTGAGGTTAAGCAAGATGAGCTTATGAAGAATAGGCCACGAGTTGATCAGGTTAAATCTACTGGAATCGCCTTTGGACCTGCGTATCAGCCGGTAACATCTTCATCTAAAGGGAAAGTGTCAAAGCTTCACAAGAGGAAGCATCAAATCACTGCGTTGTTCATGGACATGAAGCACAAGGAGTCGGAGTTGACAGAGAGACGTTCTAGAGGATTGCTCACGAAAGCCGAGACACAAGCTAAATACGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

359

Amino Acids

39.02

Weight (kDa)

7.81

Isoelectric Point (pI)

68.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRCC PF10253 161 - 359 1.1e-15 Mitotic checkpoint regulator, MAD2B-interacting
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 913
AciI CCGC 4 cut(s) 96, 384, 420, 761
AclWI GGATC 2 cut(s) 481, 487
AcuI CTGAAG 1 cut(s) 777
AdeI CACNNNGTG 1 cut(s) 455
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 2 cut(s) 53, 519
AjiI CACGTC 1 cut(s) 59
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 3 cut(s) 108, 1018, 1049
AlwI GGATC 2 cut(s) 481, 487
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 1 cut(s) 860
Asp700I GAANNNNTTC 3 cut(s) 48, 187, 663
AspS9I GGNCC 1 cut(s) 902
AsuHPI GGTGA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 1 cut(s) 902
BaeI ACNNNNGTAYC 2 cut(s) 893, 926
BauI CACGAG 1 cut(s) 864
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 460
BccI CCATC 5 cut(s) 146, 364, 641, 686, 1068
BciVI GTATCC 1 cut(s) 647
BclI TGATCA 2 cut(s) 553, 871
BcoDI GTCTC 3 cut(s) 108, 1018, 1049
BfaI CTAG 5 cut(s) 303, 351, 452, 669, 1032
BfuAI ACCTGC 1 cut(s) 913
BfuI GTATCC 1 cut(s) 647
BglII AGATCT 1 cut(s) 46
Bme18I GGWCC 1 cut(s) 902
BmeT110I CYCGRG 1 cut(s) 177
BmgBI CACGTC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 902
BmsI GCATC 3 cut(s) 395, 804, 976
BpiI GAAGAC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 1026, 1058
BsaBI GATNNNNATC 1 cut(s) 478
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 3 cut(s) 293, 643, 892
Bse3DI GCAATG 1 cut(s) 747
Bse8I GATNNNNATC 1 cut(s) 478
BseGI GGATG 1 cut(s) 1079
BseJI GATNNNNATC 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 2 cut(s) 99, 126
BseNI ACTGG 3 cut(s) 293, 643, 892
BseRI GAGGAG 2 cut(s) 204, 802
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 1 cut(s) 862
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 917
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 3 cut(s) 108, 1018, 1049
BsmBI CGTCTC 2 cut(s) 108, 1018
BsnI GGCC 1 cut(s) 862
BsoBI CYCGRG 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
BspACI CCGC 4 cut(s) 96, 384, 420, 761
BspANI GGCC 1 cut(s) 862
BspCNI CTCAG 2 cut(s) 98, 125
BspMI ACCTGC 1 cut(s) 913
BspPI GGATC 2 cut(s) 481, 487
BspQI GCTCTTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 1 cut(s) 916
BsrI ACTGG 3 cut(s) 293, 643, 892
BssAI RCCGGY 1 cut(s) 916
BssMI GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
BssSI CACGAG 1 cut(s) 864
Bst2BI CACGAG 1 cut(s) 864
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 2 cut(s) 336, 465
BstC8I GCNNGC 2 cut(s) 19, 429
BstDEI CTNAG 2 cut(s) 85, 112
BstF5I GGATG 1 cut(s) 1079
BstKTI GATC 8 cut(s) 49, 285, 410, 476, 482, 494, 556, 874
BstMAI GTCTC 3 cut(s) 108, 1018, 1049
BstMBI GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
BstMWI GCNNNNNNNGC 4 cut(s) 23, 428, 767, 844
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 1 cut(s) 862
BtrI CACGTC 1 cut(s) 59
BtsCI GGATG 1 cut(s) 1079
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 2 cut(s) 72, 975
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 2 cut(s) 19, 429
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 1 cut(s) 902
CviAII CATG 3 cut(s) 424, 988, 994
DdeI CTNAG 2 cut(s) 85, 112
DpnI GATC 8 cut(s) 48, 284, 409, 475, 481, 493, 555, 873
DpnII GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
DraIII CACNNNGTG 1 cut(s) 455
Eam1104I CTCTTC 2 cut(s) 336, 465
EarI CTCTTC 2 cut(s) 336, 465
EciI GGCGGA 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 902
Eco57I CTGAAG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 177
Esp3I CGTCTC 2 cut(s) 108, 1018
FaeI CATG 3 cut(s) 427, 991, 997
FalI AAGNNNNNCTT 4 cut(s) 33, 65, 831, 863
FatI CATG 3 cut(s) 423, 987, 993
FbaI TGATCA 2 cut(s) 553, 871
FspBI CTAG 5 cut(s) 303, 351, 452, 669, 1032
HaeIII GGCC 1 cut(s) 862
HapII CCGG 1 cut(s) 917
Hin1II CATG 3 cut(s) 427, 991, 997
HincII GTYRAC 1 cut(s) 1017
HindII GTYRAC 1 cut(s) 1017
HindIII AAGCTT 2 cut(s) 66, 950
HinfI GANTC 9 cut(s) 5, 374, 395, 496, 539, 575, 808, 891, 1007
HpaII CCGG 1 cut(s) 917
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 3 cut(s) 298, 365, 1017
Hpy188I TCNGA 6 cut(s) 34, 88, 148, 267, 813, 1012
Hpy188III TCNNGA 9 cut(s) 62, 177, 280, 317, 392, 477, 500, 1032, 1045
Hpy8I GTNNAC 3 cut(s) 298, 365, 1017
Hpy99I CGWCG 5 cut(s) 34, 63, 259, 326, 335
HpyAV CCTTC 4 cut(s) 102, 474, 525, 789
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 3 cut(s) 58, 271, 1027
HpyCH4V TGCA 2 cut(s) 431, 524
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 428, 767, 844
HpyF3I CTNAG 2 cut(s) 85, 112
HpySE526I ACGT 3 cut(s) 58, 271, 1027
Hsp92II CATG 3 cut(s) 427, 991, 997
Ksp22I TGATCA 2 cut(s) 553, 871
Kzo9I GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
LguI GCTCTTC 1 cut(s) 465
LweI GCATC 3 cut(s) 395, 804, 976
MaeI CTAG 5 cut(s) 303, 351, 452, 669, 1032
MaeII ACGT 3 cut(s) 58, 271, 1027
MaeIII GTNAC 1 cut(s) 919
MalI GATC 8 cut(s) 48, 284, 409, 475, 481, 493, 555, 873
MboI GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 4 cut(s) 228, 261, 519, 663
MlyI GAGTC 1 cut(s) 1016
MmeI TCCRAC 3 cut(s) 12, 595, 990
MroXI GAANNNNTTC 3 cut(s) 48, 187, 663
MseI TTAA 2 cut(s) 834, 879
MslI CAYNNNNRTG 1 cut(s) 992
MspI CCGG 1 cut(s) 917
MunI CAATTG 1 cut(s) 519
MwoI GCNNNNNNNGC 4 cut(s) 23, 428, 767, 844
NdeII GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
NlaIII CATG 3 cut(s) 427, 991, 997
NmeAIII GCCGAG 1 cut(s) 1078
PaeR7I CTCGAG 1 cut(s) 177
PciSI GCTCTTC 1 cut(s) 465
PcsI WCGNNNNNNNCGW 3 cut(s) 263, 327, 330
PdmI GAANNNNTTC 3 cut(s) 48, 187, 663
PfeI GAWTC 7 cut(s) 374, 395, 496, 539, 575, 808, 891
PleI GAGTC 1 cut(s) 1015
PpsI GAGTC 1 cut(s) 1015
PspPI GGNCC 1 cut(s) 902
PsuI RGATCY 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 992
SapI GCTCTTC 1 cut(s) 465
SaqAI TTAA 2 cut(s) 834, 879
Sau3AI GATC 8 cut(s) 46, 282, 407, 473, 479, 491, 553, 871
Sau96I GGNCC 1 cut(s) 902
SchI GAGTC 1 cut(s) 1016
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 3 cut(s) 395, 804, 976
Sfr274I CTCGAG 1 cut(s) 177
SinI GGWCC 1 cut(s) 902
SlaI CTCGAG 1 cut(s) 177
SmiMI CAYNNNNRTG 1 cut(s) 992
SmlI CTYRAG 1 cut(s) 177
SmoI CTYRAG 1 cut(s) 177
Sse9I AATT 4 cut(s) 228, 261, 519, 663
SsiI CCGC 4 cut(s) 96, 384, 420, 761
SspMI CTAG 5 cut(s) 303, 351, 452, 669, 1032
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 3 cut(s) 61, 274, 1030
TaqI TCGA 5 cut(s) 61, 178, 487, 494, 501
TaqII GACCGA 1 cut(s) 322
TasI AATT 4 cut(s) 228, 261, 519, 663
TfiI GAWTC 7 cut(s) 374, 395, 496, 539, 575, 808, 891
Tru1I TTAA 2 cut(s) 834, 879
Tru9I TTAA 2 cut(s) 834, 879
TscAI CASTG 2 cut(s) 79, 982
TspDTI ATGAA 7 cut(s) 387, 456, 588, 866, 918, 976, 1010
TspGWI ACGGA 4 cut(s) 243, 250, 551, 617
TspRI CASTG 2 cut(s) 79, 982
VpaK11BI GGWCC 1 cut(s) 902
XbaI TCTAGA 1 cut(s) 1031
XhoI CTCGAG 1 cut(s) 177
XmnI GAANNNNTTC 3 cut(s) 48, 187, 663
XspI CTAG 5 cut(s) 303, 351, 452, 669, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.