Rh4DG086200

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016126
Physical Location & Seq
Forward (+)
13104875 .. 13108380
3506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG086200.1

Sequence Viewer

Length: 1290 bp
ATGGACTCTCTGCTAGCAAACTACGCCTCTTCCGACGAAGAAGAAGATCAAAAACCCCACCACCACCAACTACAACCACCTCCAAACCCTTCTTCTTCTTCTTCTCTTTTCTCTTCTCTACCCAAACCCAAACCCAAACCCGAACCCTCATCCTTCTTCAATTCCCTTCCTCCTCCAAAACAAACCCAATCAAATCCTCCCCACCAAGAAGACCAAGACTCAAAACCCACCTCAAGATCTTCTCTTTTCTCCTCTCTGCCCAAACCCAAACCCTCCTCTCTCTTCCAATCCCTTCCCAAACCAAAACTTGAACCAAAACAAGAAGAAGAAGAAGAAGAAGAAGAAGACATAAAACCCCCTTCTTCTGTTTTCTCTTCTCTTCCTCCCCCAAAATCCCAAATCCCTAACAAACCCATCTCCAATTCCTCATCTTCTCCTGCCCAAATCCCCAAAAGGGTCGTCCAATTCAAGCCTCCCATCAACCCTTATTTCGCAAACTCTTCCCATCTCGAAGACGACGACGACGATGAGGAAGAAGAAGAAGAGAGGCGGCGGCGAAAAGCCTCGGAGGCCTCGTCGGCGCAACCTTTGTCCGTGACGTCGTTCTTATCAGCTATTCCGGCGCCCAGGAACTCGGGTGTGAATTCCGGTTTAGGTTCCGGTGTAGGTTCCGGTAGGAGAGCCACCGTTGAAACAGAGTCGTTTGGTTCTAAAGTGGAGAGCGATGTGGGTTTGGATCGAAATGAAAATGTAGCAAGTTATGATGATCCCCAATCAAATTTTGATCCAAATGCAGAAGGTTATGGTGGGTATGAGAGTTACCAATATGGAGTTGATCAAAATGTGGATTCTGGGGTTAGTGGCAGCGATGCTTTAAGCTATGGTAATTACAGTGGTAATGGTGGGTATAGTGGTTATGCTGGAAATCATGGGAACGAATGGGCTGATGGGTCGGGGAGTGCGGCGGCAATGGGTATGTCAGGTGATACTGGGGTTAGAGTGAGTGGGAAGAGGAGGAGGAATGATGTCCCAACTGAAATACTTGAGGTTAAGCAGGATGAGTTGATGAAGAATCGGCCGAGGGAGGACCAATCCAAGGTGACCGGGATAGCTTTTGGGCCTTCTTACCAGCCTGTTTCGACGAAGGGGAAGCCGACAAAGCTGCATAAGAGGAAGCATCAGATTGGTTCTCTGTACTTTGATATGAAGCAGAAGGAGATGGAACTTCAGGAGCGGCGTTCAAGAGGCTTCCTTACAAAAGCTGAAACACAAGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

429

Amino Acids

46.94

Weight (kDa)

5.41

Isoelectric Point (pI)

69.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 602
AccB1I GGYRCC 1 cut(s) 622
AccBSI CCGCTC 1 cut(s) 1234
AciI CCGC 5 cut(s) 550, 553, 962, 965, 1234
AclWI GGATC 3 cut(s) 744, 761, 779
AcoI YGGCCR 1 cut(s) 1076
AcsI RAATTY 2 cut(s) 643, 778
AcuI CTGAAG 1 cut(s) 1211
AcyI GRCGYC 2 cut(s) 599, 623
AfaI GTAC 1 cut(s) 1196
AfiI CCNNNNNNNGG 2 cut(s) 454, 1096
AgsI TTSAA 5 cut(s) 160, 311, 469, 692, 1242
AjnI CCWGG 1 cut(s) 626
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 5 cut(s) 614, 879, 1112, 1162, 1262
AluI AGCT 5 cut(s) 614, 879, 1112, 1162, 1262
AlwI GGATC 3 cut(s) 744, 761, 779
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 3 cut(s) 570, 1076, 1118
ApeKI GCWGC 2 cut(s) 864, 1162
ApoI RAATTY 2 cut(s) 643, 778
ArsI GACNNNNNNTTYG 2 cut(s) 444, 476
AspLEI GCGC 2 cut(s) 583, 625
AspS9I GGNCC 2 cut(s) 1087, 1118
AsuC2I CCSGG 1 cut(s) 1105
AsuHPI GGTGA 2 cut(s) 995, 1111
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 1 cut(s) 1087
BaeI ACNNNNGTAYC 2 cut(s) 978, 1011
BanI GGYRCC 1 cut(s) 622
BbsI GAAGAC 3 cut(s) 216, 351, 519
BbvI GCAGC 2 cut(s) 876, 1149
BccI CCATC 6 cut(s) 422, 485, 513, 941, 1213, 1278
BcgI CGANNNNNNTGC 2 cut(s) 1144, 1178
BciT130I CCWGG 1 cut(s) 628
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 1 cut(s) 1105
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 626
BglI GCCNNNNNGGC 2 cut(s) 569, 578
BglII AGATCT 1 cut(s) 236
BisI GCNGC 7 cut(s) 551, 554, 865, 963, 966, 1163, 1235
BlsI GCNGC 7 cut(s) 552, 555, 866, 964, 967, 1164, 1236
Bme1390I CCNGG 2 cut(s) 628, 1105
Bme18I GGWCC 1 cut(s) 1087
BmeT110I CYCGRG 1 cut(s) 634
BmgT120I GGNCC 2 cut(s) 1087, 1118
BmiI GGNNCC 3 cut(s) 624, 658, 670
BmrFI CCNGG 2 cut(s) 628, 1105
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 2 cut(s) 859, 1186
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 999
BpiI GAAGAC 3 cut(s) 216, 351, 519
BpuEI CTTGAG 2 cut(s) 217, 1064
BpuMI CCSGG 1 cut(s) 1105
BsaHI GRCGYC 2 cut(s) 599, 623
BsaJI CCNNGG 4 cut(s) 564, 626, 1079, 1095
BsaWI WCCGGW 3 cut(s) 647, 659, 671
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 2 cut(s) 454, 1096
Bse1I ACTGG 1 cut(s) 994
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 1 cut(s) 628
BseDI CCNNGG 4 cut(s) 564, 626, 1079, 1095
BseGI GGATG 3 cut(s) 149, 1063, 1289
BseLI CCNNNNNNNGG 2 cut(s) 454, 1096
BseMI GCAATG 1 cut(s) 975
BseNI ACTGG 1 cut(s) 994
BseRI GAGGAG 5 cut(s) 162, 241, 265, 1027, 1030
BseX3I CGGCCG 1 cut(s) 1076
BseXI GCAGC 2 cut(s) 876, 1149
Bsh1285I CGRYCG 1 cut(s) 1079
BshFI GGCC 3 cut(s) 572, 1078, 1120
BshNI GGYRCC 1 cut(s) 622
BsiEI CGRYCG 1 cut(s) 1079
BsiHKCI CYCGRG 1 cut(s) 634
BsiSI CCGG 5 cut(s) 620, 648, 660, 672, 1104
BslFI GGGAC 1 cut(s) 1013
BslI CCNNNNNNNGG 2 cut(s) 454, 1096
BsmFI GGGAC 1 cut(s) 1013
BsnI GGCC 3 cut(s) 572, 1078, 1120
BsoBI CYCGRG 1 cut(s) 634
Bsp143I GATC 6 cut(s) 46, 236, 736, 766, 784, 835
BspACI CCGC 5 cut(s) 550, 553, 962, 965, 1234
BspANI GGCC 3 cut(s) 572, 1078, 1120
BspLI GGNNCC 3 cut(s) 624, 658, 670
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 744, 761, 779
BspT107I GGYRCC 1 cut(s) 622
BsrBI CCGCTC 1 cut(s) 1234
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 1 cut(s) 994
BssECI CCNNGG 4 cut(s) 564, 626, 1079, 1095
BssMI GATC 6 cut(s) 46, 236, 736, 766, 784, 835
BssNI GRCGYC 2 cut(s) 599, 623
BssT1I CCWWGG 1 cut(s) 1095
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 2 cut(s) 688, 893
Bst6I CTCTTC 8 cut(s) 34, 118, 287, 379, 384, 505, 537, 1004
BstACI GRCGYC 2 cut(s) 599, 623
BstC8I GCNNGC 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 1099
BstF5I GGATG 3 cut(s) 149, 1063, 1289
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 2 cut(s) 583, 625
BstKTI GATC 6 cut(s) 49, 239, 739, 769, 787, 838
BstMBI GATC 6 cut(s) 46, 236, 736, 766, 784, 835
BstMCI CGRYCG 1 cut(s) 1079
BstMWI GCNNNNNNNGC 5 cut(s) 23, 569, 578, 620, 1159
BstNI CCWGG 1 cut(s) 628
BstPI GGTNACC 1 cut(s) 1099
BstSCI CCNGG 2 cut(s) 626, 1103
BstV1I GCAGC 2 cut(s) 876, 1149
BstV2I GAAGAC 3 cut(s) 216, 351, 519
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BstZI CGGCCG 1 cut(s) 1076
BsuRI GGCC 3 cut(s) 572, 1078, 1120
BtgZI GCGATG 2 cut(s) 738, 882
BtsCI GGATG 3 cut(s) 149, 1063, 1289
BtsIMutI CAGTG 1 cut(s) 898
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 583, 625
Cfr13I GGNCC 2 cut(s) 1087, 1118
Csp6I GTAC 1 cut(s) 1195
CviAII CATG 1 cut(s) 929
CviQI GTAC 1 cut(s) 1195
DinI GGCGCC 1 cut(s) 624
DpnI GATC 6 cut(s) 48, 238, 738, 768, 786, 837
DpnII GATC 6 cut(s) 46, 236, 736, 766, 784, 835
EaeI YGGCCR 1 cut(s) 1076
EagI CGGCCG 1 cut(s) 1076
Eam1104I CTCTTC 8 cut(s) 34, 118, 287, 379, 384, 505, 537, 1004
EarI CTCTTC 8 cut(s) 34, 118, 287, 379, 384, 505, 537, 1004
EclXI CGGCCG 1 cut(s) 1076
Eco130I CCWWGG 1 cut(s) 1095
Eco147I AGGCCT 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 1087
Eco52I CGGCCG 1 cut(s) 1076
Eco57I CTGAAG 1 cut(s) 1211
Eco88I CYCGRG 1 cut(s) 634
Eco91I GGTNACC 1 cut(s) 1099
EcoO65I GGTNACC 1 cut(s) 1099
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 626
EcoT14I CCWWGG 1 cut(s) 1095
EgeI GGCGCC 1 cut(s) 624
EheI GGCGCC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 1095
FaeI CATG 1 cut(s) 932
FaqI GGGAC 1 cut(s) 1013
FatI CATG 1 cut(s) 928
FbaI TGATCA 1 cut(s) 835
Fnu4HI GCNGC 7 cut(s) 551, 554, 865, 963, 966, 1163, 1235
FokI GGATG 2 cut(s) 136, 1070
Fsp4HI GCNGC 7 cut(s) 551, 554, 865, 963, 966, 1163, 1235
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 582, 624
GluI GCNGC 7 cut(s) 551, 554, 865, 963, 966, 1163, 1235
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 3 cut(s) 572, 1078, 1120
HapII CCGG 5 cut(s) 620, 648, 660, 672, 1104
HhaI GCGC 2 cut(s) 583, 625
Hin1I GRCGYC 2 cut(s) 599, 623
Hin1II CATG 1 cut(s) 932
Hin6I GCGC 2 cut(s) 581, 623
HinP1I GCGC 2 cut(s) 581, 623
HinfI GANTC 5 cut(s) 5, 218, 698, 848, 1072
HpaII CCGG 5 cut(s) 620, 648, 660, 672, 1104
HphI GGTGA 2 cut(s) 995, 1111
Hpy188I TCNGA 3 cut(s) 34, 568, 1182
Hpy188III TCNNGA 4 cut(s) 234, 509, 1229, 1242
Hpy99I CGWCG 7 cut(s) 38, 521, 524, 527, 580, 604, 1144
HpyAV CCTTC 9 cut(s) 99, 163, 176, 302, 369, 791, 1131, 1138, 1207
HpyCH4III ACNGT 2 cut(s) 688, 893
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 2 cut(s) 794, 1165
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 569, 578, 620, 1159
HpySE526I ACGT 1 cut(s) 599
Hsp92I GRCGYC 2 cut(s) 599, 623
Hsp92II CATG 1 cut(s) 932
HspAI GCGC 2 cut(s) 581, 623
KasI GGCGCC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 6 cut(s) 46, 236, 736, 766, 784, 835
LmnI GCTCC 1 cut(s) 1231
Lsp1109I GCAGC 2 cut(s) 876, 1149
LweI GCATC 2 cut(s) 859, 1186
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 3 cut(s) 595, 818, 1099
MalI GATC 6 cut(s) 48, 238, 738, 768, 786, 837
MbiI CCGCTC 1 cut(s) 1234
MboI GATC 6 cut(s) 46, 236, 736, 766, 784, 835
MflI RGATCY 1 cut(s) 236
MluCI AATT 6 cut(s) 160, 421, 464, 643, 778, 886
Mly113I GGCGCC 1 cut(s) 623
MlyI GAGTC 2 cut(s) 212, 707
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 2 cut(s) 875, 1050
MspI CCGG 5 cut(s) 620, 648, 660, 672, 1104
MspR9I CCNGG 2 cut(s) 628, 1105
MvaI CCWGG 1 cut(s) 628
MwoI GCNNNNNNNGC 5 cut(s) 23, 569, 578, 620, 1159
NarI GGCGCC 1 cut(s) 623
NciI CCSGG 1 cut(s) 1105
NdeII GATC 6 cut(s) 46, 236, 736, 766, 784, 835
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 932
NlaIV GGNNCC 3 cut(s) 624, 658, 670
NmeAIII GCCGAG 1 cut(s) 1104
NmuCI GTSAC 2 cut(s) 595, 1099
PceI AGGCCT 1 cut(s) 572
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 516, 522, 572
PfeI GAWTC 2 cut(s) 848, 1072
PkrI GCNGC 7 cut(s) 552, 555, 866, 964, 967, 1164, 1236
PleI GAGTC 2 cut(s) 212, 706
PluTI GGCGCC 1 cut(s) 626
PpsI GAGTC 2 cut(s) 212, 706
Psp6I CCWGG 1 cut(s) 626
PspEI GGTNACC 1 cut(s) 1099
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 3 cut(s) 624, 658, 670
PspPI GGNCC 2 cut(s) 1087, 1118
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 1 cut(s) 1196
RsaNI GTAC 1 cut(s) 1195
SaqAI TTAA 2 cut(s) 875, 1050
SatI GCNGC 7 cut(s) 551, 554, 865, 963, 966, 1163, 1235
Sau3AI GATC 6 cut(s) 46, 236, 736, 766, 784, 835
Sau96I GGNCC 2 cut(s) 1087, 1118
SchI GAGTC 2 cut(s) 212, 707
ScrFI CCNGG 2 cut(s) 628, 1105
SfaNI GCATC 2 cut(s) 859, 1186
SfoI GGCGCC 1 cut(s) 624
SinI GGWCC 1 cut(s) 1087
SmlI CTYRAG 2 cut(s) 232, 1043
SmoI CTYRAG 2 cut(s) 232, 1043
Sse9I AATT 6 cut(s) 160, 421, 464, 643, 778, 886
SseBI AGGCCT 1 cut(s) 572
SsiI CCGC 5 cut(s) 550, 553, 962, 965, 1234
SspDI GGCGCC 1 cut(s) 622
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 2 cut(s) 626, 1103
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 2 cut(s) 688, 893
TaiI ACGT 1 cut(s) 602
TaqI TCGA 3 cut(s) 510, 739, 1139
TasI AATT 6 cut(s) 160, 421, 464, 643, 778, 886
TatI WGTACW 1 cut(s) 1194
TauI GCSGC 5 cut(s) 553, 556, 965, 968, 1237
TfiI GAWTC 2 cut(s) 848, 1072
Tru1I TTAA 2 cut(s) 875, 1050
Tru9I TTAA 2 cut(s) 875, 1050
TscAI CASTG 1 cut(s) 898
TseFI GTSAC 2 cut(s) 595, 1099
TseI GCWGC 2 cut(s) 864, 1162
Tsp45I GTSAC 2 cut(s) 595, 1099
TspDTI ATGAA 3 cut(s) 759, 1082, 1220
TspGWI ACGGA 1 cut(s) 583
TspRI CASTG 1 cut(s) 898
VpaK11BI GGWCC 1 cut(s) 1087
XapI RAATTY 2 cut(s) 643, 778
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.