Rmu_sc0016554.1_g000003

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016554.1
OG0016126
Physical Location & Seq
Forward (+)
7455 .. 11271
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016554.1_g000003.1.cds

Sequence Viewer

Length: 1272 bp
atggactctctgctagcaaactacgcctcttccgacgaagaagaagatcaaaaaccccaccaccaccaactacaaccacctccaaacgcttcttcttctcttttctcttctctacccaaacccaaacccgaacctttatccttcttcaattcccttcctcctccaaaacaaacccaatcaaatcctccccaccaagaagaccaagactcaaaacccacctcaagatcttctcttttctcctctctacccaaacccaaaccctcctctctcttccaatcccttcccaaaccaaaacttgaaccacaacaagatgaagaagaagaagaagacataaaacccccttcttctgttttctcttctcttcctccccccaaatcccaaatccctaacaaacccatctccaattcctcatcttctcctgcccaaatccccaaaagggtcgtccaattcaagcctcccgtcaacccttatttcgcgaactcttcccatctcgaagacgacgacgacgacgatgaggaagaagaagagaggcggcggcgaaaagcctcggaggcctcgtcggcgcaacctttgtccgtgacgtccttcttatcagctattccggcgcccaggaactcgggtgtgaattccggtttaggttccggtgtaggttccggtaggagagccaccgttgaaactgagtcgtttggttctaaagtggagagcgatgtgggtttggatcgaaatgaaaatgtagcaagttatgatgatccccaatcaaattttgatccaaatgcagaaggttatggtgggtatgagagttaccaatatggtgttgaccaaaatgtggattctggggttagtggcggtgatggttcgagctatggtaattacagtggttatggtgggtataatggttatgctggaaatcatgggaacgaatgggctgatgggtcggggactgcggcggcaatgggtgtgtcaggtgatactggggttaaactgagtgggaagaggaggaggaatgatgtcccaactgaaatacttgaggttaagcaggatgagttgatgaagaatcggccgagggaggaccaagccaaggtgaccgggatagcttttgggccttcttaccagcctgtttcgacgaaggggaagccgacaaagctgcataagaggaagcatcagattggttctctgtactttgatatgaagcagaaggagatggaacttcaggagcggcgttcaagaggcttccttacaaaagctgaaacacaagccaagtatggatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

423

Amino Acids

46.2

Weight (kDa)

5.38

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 584
AccB1I GGYRCC 1 cut(s) 604
AccB7I CCANNNNNTGG 1 cut(s) 826
AccBSI CCGCTC 1 cut(s) 1216
AccII CGCG 1 cut(s) 476
AciI CCGC 6 cut(s) 532, 535, 846, 944, 947, 1216
AclWI GGATC 3 cut(s) 726, 743, 761
AcoI YGGCCR 1 cut(s) 1058
AcsI RAATTY 2 cut(s) 625, 760
AcuI CTGAAG 1 cut(s) 1193
AcyI GRCGYC 2 cut(s) 581, 605
AfaI GTAC 1 cut(s) 1178
AfiI CCNNNNNNNGG 3 cut(s) 436, 826, 1078
AgsI TTSAA 5 cut(s) 148, 299, 451, 674, 1224
AjnI CCWGG 1 cut(s) 608
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 5 cut(s) 596, 861, 1094, 1144, 1244
AluI AGCT 5 cut(s) 596, 861, 1094, 1144, 1244
AlwI GGATC 3 cut(s) 726, 743, 761
Ama87I CYCGRG 1 cut(s) 616
AoxI GGCC 3 cut(s) 552, 1058, 1100
ApeKI GCWGC 1 cut(s) 1144
ApoI RAATTY 2 cut(s) 625, 760
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AspLEI GCGC 2 cut(s) 565, 607
AspS9I GGNCC 2 cut(s) 1069, 1100
AsuC2I CCSGG 1 cut(s) 1087
AsuHPI GGTGA 3 cut(s) 860, 977, 1093
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 616
AvaII GGWCC 1 cut(s) 1069
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BanI GGYRCC 1 cut(s) 604
BbsI GAAGAC 3 cut(s) 204, 333, 501
BbvI GCAGC 1 cut(s) 1131
BccI CCATC 6 cut(s) 404, 495, 845, 923, 1195, 1260
BcgI CGANNNNNNTGC 2 cut(s) 1126, 1160
BciT130I CCWGG 1 cut(s) 610
BcnI CCSGG 1 cut(s) 1087
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 608
BglI GCCNNNNNGGC 2 cut(s) 551, 560
BglII AGATCT 1 cut(s) 224
BisI GCNGC 6 cut(s) 533, 536, 945, 948, 1145, 1217
BlsI GCNGC 6 cut(s) 534, 537, 946, 949, 1146, 1218
Bme1390I CCNGG 2 cut(s) 610, 1087
Bme18I GGWCC 1 cut(s) 1069
BmeT110I CYCGRG 1 cut(s) 616
BmgT120I GGNCC 2 cut(s) 1069, 1100
BmiI GGNNCC 3 cut(s) 606, 640, 652
BmrFI CCNGG 2 cut(s) 610, 1087
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 1 cut(s) 1168
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 981
BpiI GAAGAC 3 cut(s) 204, 333, 501
BpuEI CTTGAG 2 cut(s) 205, 1046
BpuMI CCSGG 1 cut(s) 1087
BsaHI GRCGYC 2 cut(s) 581, 605
BsaJI CCNNGG 4 cut(s) 546, 608, 1061, 1077
BsaWI WCCGGW 3 cut(s) 629, 641, 653
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 3 cut(s) 436, 826, 1078
Bse1I ACTGG 1 cut(s) 976
Bse3DI GCAATG 1 cut(s) 957
BseBI CCWGG 1 cut(s) 610
BseDI CCNNGG 4 cut(s) 546, 608, 1061, 1077
BseGI GGATG 2 cut(s) 1045, 1271
BseLI CCNNNNNNNGG 3 cut(s) 436, 826, 1078
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 2 cut(s) 669, 974
BseNI ACTGG 1 cut(s) 976
BseRI GAGGAG 5 cut(s) 150, 229, 253, 1009, 1012
BseX3I CGGCCG 1 cut(s) 1058
BseXI GCAGC 1 cut(s) 1131
Bsh1236I CGCG 1 cut(s) 476
Bsh1285I CGRYCG 1 cut(s) 1061
BshFI GGCC 3 cut(s) 554, 1060, 1102
BshNI GGYRCC 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 1061
BsiHKCI CYCGRG 1 cut(s) 616
BsiSI CCGG 5 cut(s) 602, 630, 642, 654, 1086
BslFI GGGAC 2 cut(s) 952, 995
BslI CCNNNNNNNGG 3 cut(s) 436, 826, 1078
BsmFI GGGAC 2 cut(s) 952, 995
BsnI GGCC 3 cut(s) 554, 1060, 1102
BsoBI CYCGRG 1 cut(s) 616
Bsp143I GATC 5 cut(s) 46, 224, 718, 748, 766
Bsp68I TCGCGA 1 cut(s) 476
BspACI CCGC 6 cut(s) 532, 535, 846, 944, 947, 1216
BspANI GGCC 3 cut(s) 554, 1060, 1102
BspCNI CTCAG 2 cut(s) 670, 975
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 3 cut(s) 606, 640, 652
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 726, 743, 761
BspT107I GGYRCC 1 cut(s) 604
BsrBI CCGCTC 1 cut(s) 1216
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 1 cut(s) 976
BssECI CCNNGG 4 cut(s) 546, 608, 1061, 1077
BssMI GATC 5 cut(s) 46, 224, 718, 748, 766
BssNI GRCGYC 2 cut(s) 581, 605
BssT1I CCWWGG 1 cut(s) 1077
Bst2UI CCWGG 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 670, 875
Bst6I CTCTTC 8 cut(s) 34, 112, 275, 361, 366, 487, 519, 986
BstACI GRCGYC 2 cut(s) 581, 605
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 678, 983
BstEII GGTNACC 1 cut(s) 1081
BstF5I GGATG 2 cut(s) 1045, 1271
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 2 cut(s) 565, 607
BstKTI GATC 5 cut(s) 49, 227, 721, 751, 769
BstMBI GATC 5 cut(s) 46, 224, 718, 748, 766
BstMCI CGRYCG 1 cut(s) 1061
BstMWI GCNNNNNNNGC 5 cut(s) 23, 551, 560, 602, 1141
BstNI CCWGG 1 cut(s) 610
BstPI GGTNACC 1 cut(s) 1081
BstSCI CCNGG 2 cut(s) 608, 1085
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 1131
BstV2I GAAGAC 3 cut(s) 204, 333, 501
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZI CGGCCG 1 cut(s) 1058
BsuRI GGCC 3 cut(s) 554, 1060, 1102
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 2 cut(s) 1045, 1271
BtsIMutI CAGTG 1 cut(s) 880
BtuMI TCGCGA 1 cut(s) 476
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 565, 607
Cfr13I GGNCC 2 cut(s) 1069, 1100
Csp6I GTAC 1 cut(s) 1177
CviAII CATG 1 cut(s) 911
CviQI GTAC 1 cut(s) 1177
DdeI CTNAG 2 cut(s) 678, 983
DinI GGCGCC 1 cut(s) 606
DpnI GATC 5 cut(s) 48, 226, 720, 750, 768
DpnII GATC 5 cut(s) 46, 224, 718, 748, 766
EaeI YGGCCR 1 cut(s) 1058
EagI CGGCCG 1 cut(s) 1058
Eam1104I CTCTTC 8 cut(s) 34, 112, 275, 361, 366, 487, 519, 986
EarI CTCTTC 8 cut(s) 34, 112, 275, 361, 366, 487, 519, 986
EclXI CGGCCG 1 cut(s) 1058
Eco130I CCWWGG 1 cut(s) 1077
Eco147I AGGCCT 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 1069
Eco52I CGGCCG 1 cut(s) 1058
Eco57I CTGAAG 1 cut(s) 1193
Eco88I CYCGRG 1 cut(s) 616
Eco91I GGTNACC 1 cut(s) 1081
EcoO65I GGTNACC 1 cut(s) 1081
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 1077
EgeI GGCGCC 1 cut(s) 606
EheI GGCGCC 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 1077
FaeI CATG 1 cut(s) 914
FaqI GGGAC 2 cut(s) 952, 995
FatI CATG 1 cut(s) 910
Fnu4HI GCNGC 6 cut(s) 533, 536, 945, 948, 1145, 1217
FokI GGATG 1 cut(s) 1052
Fsp4HI GCNGC 6 cut(s) 533, 536, 945, 948, 1145, 1217
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 564, 606
GluI GCNGC 6 cut(s) 533, 536, 945, 948, 1145, 1217
HaeII RGCGCY 1 cut(s) 608
HaeIII GGCC 3 cut(s) 554, 1060, 1102
HapII CCGG 5 cut(s) 602, 630, 642, 654, 1086
HhaI GCGC 2 cut(s) 565, 607
Hin1I GRCGYC 2 cut(s) 581, 605
Hin1II CATG 1 cut(s) 914
Hin6I GCGC 2 cut(s) 563, 605
HinP1I GCGC 2 cut(s) 563, 605
HincII GTYRAC 2 cut(s) 463, 817
HindII GTYRAC 2 cut(s) 463, 817
HinfI GANTC 5 cut(s) 5, 206, 680, 830, 1054
HpaII CCGG 5 cut(s) 602, 630, 642, 654, 1086
HphI GGTGA 3 cut(s) 860, 977, 1093
Hpy166II GTNNAC 2 cut(s) 463, 817
Hpy188I TCNGA 3 cut(s) 34, 550, 1164
Hpy188III TCNNGA 5 cut(s) 222, 475, 491, 1211, 1224
Hpy8I GTNNAC 2 cut(s) 463, 817
Hpy99I CGWCG 7 cut(s) 38, 503, 506, 509, 512, 562, 1126
HpyAV CCTTC 9 cut(s) 151, 164, 290, 351, 595, 773, 1113, 1120, 1189
HpyCH4III ACNGT 2 cut(s) 670, 875
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 2 cut(s) 776, 1147
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 551, 560, 602, 1141
HpyF3I CTNAG 2 cut(s) 678, 983
HpySE526I ACGT 1 cut(s) 581
Hsp92I GRCGYC 2 cut(s) 581, 605
Hsp92II CATG 1 cut(s) 914
HspAI GCGC 2 cut(s) 563, 605
KasI GGCGCC 1 cut(s) 604
Kzo9I GATC 5 cut(s) 46, 224, 718, 748, 766
LmnI GCTCC 1 cut(s) 1213
Lsp1109I GCAGC 1 cut(s) 1131
LweI GCATC 1 cut(s) 1168
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 3 cut(s) 577, 800, 1081
MalI GATC 5 cut(s) 48, 226, 720, 750, 768
MbiI CCGCTC 1 cut(s) 1216
MboI GATC 5 cut(s) 46, 224, 718, 748, 766
MflI RGATCY 1 cut(s) 224
MluCI AATT 6 cut(s) 148, 403, 446, 625, 760, 868
Mly113I GGCGCC 1 cut(s) 605
MlyI GAGTC 2 cut(s) 200, 689
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 2 cut(s) 978, 1032
MspI CCGG 5 cut(s) 602, 630, 642, 654, 1086
MspR9I CCNGG 2 cut(s) 610, 1087
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 5 cut(s) 23, 551, 560, 602, 1141
NarI GGCGCC 1 cut(s) 605
NciI CCSGG 1 cut(s) 1087
NdeII GATC 5 cut(s) 46, 224, 718, 748, 766
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 914
NlaIV GGNNCC 3 cut(s) 606, 640, 652
NmeAIII GCCGAG 1 cut(s) 1086
NmuCI GTSAC 2 cut(s) 577, 1081
NruI TCGCGA 1 cut(s) 476
PceI AGGCCT 1 cut(s) 554
PcsI WCGNNNNNNNCGW 5 cut(s) 30, 498, 504, 507, 554
PfeI GAWTC 2 cut(s) 830, 1054
PflMI CCANNNNNTGG 1 cut(s) 826
PkrI GCNGC 6 cut(s) 534, 537, 946, 949, 1146, 1218
PleI GAGTC 2 cut(s) 200, 688
PluTI GGCGCC 1 cut(s) 608
PpsI GAGTC 2 cut(s) 200, 688
Psp6I CCWGG 1 cut(s) 608
PspEI GGTNACC 1 cut(s) 1081
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 3 cut(s) 606, 640, 652
PspPI GGNCC 2 cut(s) 1069, 1100
PsuI RGATCY 1 cut(s) 224
RruI TCGCGA 1 cut(s) 476
RsaI GTAC 1 cut(s) 1178
RsaNI GTAC 1 cut(s) 1177
SaqAI TTAA 2 cut(s) 978, 1032
SatI GCNGC 6 cut(s) 533, 536, 945, 948, 1145, 1217
Sau3AI GATC 5 cut(s) 46, 224, 718, 748, 766
Sau96I GGNCC 2 cut(s) 1069, 1100
SchI GAGTC 2 cut(s) 200, 689
ScrFI CCNGG 2 cut(s) 610, 1087
SfaNI GCATC 1 cut(s) 1168
SfoI GGCGCC 1 cut(s) 606
SinI GGWCC 1 cut(s) 1069
SmlI CTYRAG 2 cut(s) 220, 1025
SmoI CTYRAG 2 cut(s) 220, 1025
Sse9I AATT 6 cut(s) 148, 403, 446, 625, 760, 868
SseBI AGGCCT 1 cut(s) 554
SsiI CCGC 6 cut(s) 532, 535, 846, 944, 947, 1216
SspDI GGCGCC 1 cut(s) 604
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 554
StyD4I CCNGG 2 cut(s) 608, 1085
StyI CCWWGG 1 cut(s) 1077
TaaI ACNGT 2 cut(s) 670, 875
TaiI ACGT 1 cut(s) 584
TaqI TCGA 4 cut(s) 492, 721, 857, 1121
TasI AATT 6 cut(s) 148, 403, 446, 625, 760, 868
TatI WGTACW 1 cut(s) 1176
TauI GCSGC 5 cut(s) 535, 538, 947, 950, 1219
TfiI GAWTC 2 cut(s) 830, 1054
Tru1I TTAA 2 cut(s) 978, 1032
Tru9I TTAA 2 cut(s) 978, 1032
TscAI CASTG 1 cut(s) 880
TseFI GTSAC 2 cut(s) 577, 1081
TseI GCWGC 1 cut(s) 1144
Tsp45I GTSAC 2 cut(s) 577, 1081
TspDTI ATGAA 4 cut(s) 327, 741, 1064, 1202
TspGWI ACGGA 1 cut(s) 565
TspRI CASTG 1 cut(s) 880
Van91I CCANNNNNTGG 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 1069
XapI RAATTY 2 cut(s) 625, 760
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.