Rh4BG091500

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016126
Physical Location & Seq
Forward (+)
15721386 .. 15724478
3093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG091500.1

Sequence Viewer

Length: 1275 bp
ATGGACTCTCTGCTAGCAAACTACGCCTCTTCCGACGAAGAAGAAGATCAAAAACCCCACCACCATCAACTACAACCACCTCCAAACCCTTCTTCTTCTTCTTCTTCTTCTCTTTTCTCTTCTCTACCCAAACCCAAACCCGAACCTTCATCCTTCTTCAATTCCCTTCCTCCTCCAAAACAAACCCAATCAAATCCTCCCCACCAAGAAGACCAAGACTCAAAACCCACCTCAAGATCTTCTCTTTTCTCCTCTCTACCCAAACCCAAACCCAAACCCTCCTCTCTCTTCCAATCCCTTCCCAAACCAAAACAAGAAGAAGAAGAAGAAGACATAAAACCCCCTTCTTCTGTTTTCTCTTCTCTTCCTCCCCCCAAATCCCAAATCCCTAACAAACCCATCTCCAATTCCTCATCTTCTCCTGCCCAAATCCCCAAAAGGGTCGTCCAATTCAAGCCTCCCGTCAACCCTTATTTCGCCAACTCTTCCCATCTCGAAGACGACGACGATGATGAGGAAGAAGAAGAAGAGAGGCGGCGGCGAAAAGCCTCGGAGGCCTCGTCGGCGCAACCTTTGTCCGTGACGTCGTTCTTATCAGCTATTCCGGCGCCCAGGAACTCGGGTGTGAATTCCGGTTTAGGTTCCGGTGTAGGTTCCGGTAGGAGAGCCACCGTTGAAACAGAGTCGTTTGGTTCTAAAGTGGAGAGCGATGTGGGTTTGGATCAAAATGAAAATGTAGCAAGTTATGATGATCCCCAATCAAATTTTGATCCAAATGCAGAAGGTTATGGTGGGTATGAGAGTTACCAATATGGTGTGGACCAAAATGTGGATTCTGGGGTTAGTCGTGGTGATGGTTCGAGCTATGGTAATTACAGTGGTTATGGTGGGTATAATGGTTATGCTGGAAATCATGGGAACGAATGGGCTGATGGGTCGGGGACTGCGGCGGCAATGGGTGTGTCAGGTGATACTGGGGTTAAAGTGAGTGGGAAGAGGAGGAGGAATGATGTCCCAACTGAAATACTTGAGGTTAAGCAGGATGAGTTGATGAAGAATCGGCCGAGGGAGGACCAAGCCAAGGTGACCGGGATAGCTTTTGGGCCTTCTTACCAGCCTGTTTCGACGAAGGGGAAGCCGACAAAGCTGCATAAGAGGAAGCATCAGATCGGTTCTCTGTACTTTGATATGAAGCAGAAGGAGATGGAACTTCAGGAGCGGCGTTCAAGAGGCTTCCTTACAAAAGCTGAAACACAAGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

424

Amino Acids

46.26

Weight (kDa)

5.65

Isoelectric Point (pI)

66.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 607
AccB7I CCANNNNNTGG 1 cut(s) 829
AccBSI CCGCTC 1 cut(s) 1219
AciI CCGC 5 cut(s) 535, 538, 947, 950, 1219
AclWI GGATC 3 cut(s) 729, 746, 764
AcoI YGGCCR 1 cut(s) 1061
AcsI RAATTY 2 cut(s) 628, 763
AcuI CTGAAG 1 cut(s) 1196
AcyI GRCGYC 2 cut(s) 584, 608
AfaI GTAC 1 cut(s) 1181
AfiI CCNNNNNNNGG 3 cut(s) 439, 829, 1081
AgsI TTSAA 4 cut(s) 160, 454, 677, 1227
AjnI CCWGG 1 cut(s) 611
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 5 cut(s) 599, 864, 1097, 1147, 1247
AluI AGCT 5 cut(s) 599, 864, 1097, 1147, 1247
AlwI GGATC 3 cut(s) 729, 746, 764
Ama87I CYCGRG 1 cut(s) 619
AoxI GGCC 3 cut(s) 555, 1061, 1103
ApeKI GCWGC 1 cut(s) 1147
ApoI RAATTY 2 cut(s) 628, 763
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AspLEI GCGC 2 cut(s) 568, 610
AspS9I GGNCC 3 cut(s) 820, 1072, 1103
AsuC2I CCSGG 1 cut(s) 1090
AsuHPI GGTGA 3 cut(s) 863, 980, 1096
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 619
AvaII GGWCC 2 cut(s) 820, 1072
BaeI ACNNNNGTAYC 2 cut(s) 963, 996
BanI GGYRCC 1 cut(s) 607
BbsI GAAGAC 3 cut(s) 216, 336, 504
BbvI GCAGC 1 cut(s) 1134
BccI CCATC 7 cut(s) 72, 407, 498, 848, 926, 1198, 1263
BcgI CGANNNNNNTGC 2 cut(s) 1129, 1163
BciT130I CCWGG 1 cut(s) 613
BcnI CCSGG 1 cut(s) 1090
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 611
BglI GCCNNNNNGGC 2 cut(s) 554, 563
BglII AGATCT 1 cut(s) 236
BisI GCNGC 6 cut(s) 536, 539, 948, 951, 1148, 1220
BlsI GCNGC 6 cut(s) 537, 540, 949, 952, 1149, 1221
Bme1390I CCNGG 2 cut(s) 613, 1090
Bme18I GGWCC 2 cut(s) 820, 1072
BmeT110I CYCGRG 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 820, 1072, 1103
BmiI GGNNCC 3 cut(s) 609, 643, 655
BmrFI CCNGG 2 cut(s) 613, 1090
BmrI ACTGGG 1 cut(s) 984
BmsI GCATC 1 cut(s) 1171
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 984
BpiI GAAGAC 3 cut(s) 216, 336, 504
BpuEI CTTGAG 2 cut(s) 217, 1049
BpuMI CCSGG 1 cut(s) 1090
BsaHI GRCGYC 2 cut(s) 584, 608
BsaJI CCNNGG 4 cut(s) 549, 611, 1064, 1080
BsaWI WCCGGW 3 cut(s) 632, 644, 656
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 3 cut(s) 439, 829, 1081
Bse1I ACTGG 1 cut(s) 979
Bse3DI GCAATG 1 cut(s) 960
BseBI CCWGG 1 cut(s) 613
BseDI CCNNGG 4 cut(s) 549, 611, 1064, 1080
BseGI GGATG 3 cut(s) 149, 1048, 1274
BseLI CCNNNNNNNGG 3 cut(s) 439, 829, 1081
BseMI GCAATG 1 cut(s) 960
BseNI ACTGG 1 cut(s) 979
BseRI GAGGAG 5 cut(s) 162, 241, 271, 1012, 1015
BseX3I CGGCCG 1 cut(s) 1061
BseXI GCAGC 1 cut(s) 1134
Bsh1285I CGRYCG 1 cut(s) 1064
BshFI GGCC 3 cut(s) 557, 1063, 1105
BshNI GGYRCC 1 cut(s) 607
BsiEI CGRYCG 1 cut(s) 1064
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 5 cut(s) 605, 633, 645, 657, 1089
BslFI GGGAC 2 cut(s) 955, 998
BslI CCNNNNNNNGG 3 cut(s) 439, 829, 1081
BsmFI GGGAC 2 cut(s) 955, 998
BsnI GGCC 3 cut(s) 557, 1063, 1105
BsoBI CYCGRG 1 cut(s) 619
Bsp143I GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
BspACI CCGC 5 cut(s) 535, 538, 947, 950, 1219
BspANI GGCC 3 cut(s) 557, 1063, 1105
BspLI GGNNCC 3 cut(s) 609, 643, 655
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 729, 746, 764
BspT107I GGYRCC 1 cut(s) 607
BsrBI CCGCTC 1 cut(s) 1219
BsrDI GCAATG 1 cut(s) 960
BsrI ACTGG 1 cut(s) 979
BssECI CCNNGG 4 cut(s) 549, 611, 1064, 1080
BssMI GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
BssNI GRCGYC 2 cut(s) 584, 608
BssT1I CCWWGG 1 cut(s) 1080
Bst2UI CCWGG 1 cut(s) 613
Bst4CI ACNGT 2 cut(s) 673, 878
Bst6I CTCTTC 8 cut(s) 34, 124, 293, 364, 369, 490, 522, 989
BstACI GRCGYC 2 cut(s) 584, 608
BstC8I GCNNGC 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 1084
BstF5I GGATG 3 cut(s) 149, 1048, 1274
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 2 cut(s) 568, 610
BstKTI GATC 6 cut(s) 49, 239, 724, 754, 772, 1170
BstMBI GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
BstMCI CGRYCG 1 cut(s) 1064
BstMWI GCNNNNNNNGC 5 cut(s) 23, 554, 563, 605, 1144
BstNI CCWGG 1 cut(s) 613
BstPI GGTNACC 1 cut(s) 1084
BstSCI CCNGG 2 cut(s) 611, 1088
BstV1I GCAGC 1 cut(s) 1134
BstV2I GAAGAC 3 cut(s) 216, 336, 504
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BstZI CGGCCG 1 cut(s) 1061
BsuRI GGCC 3 cut(s) 557, 1063, 1105
BtgZI GCGATG 1 cut(s) 723
BtsCI GGATG 3 cut(s) 149, 1048, 1274
BtsIMutI CAGTG 1 cut(s) 883
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 568, 610
Cfr13I GGNCC 3 cut(s) 820, 1072, 1103
Csp6I GTAC 1 cut(s) 1180
CviAII CATG 1 cut(s) 914
CviQI GTAC 1 cut(s) 1180
DinI GGCGCC 1 cut(s) 609
DpnI GATC 6 cut(s) 48, 238, 723, 753, 771, 1169
DpnII GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
EaeI YGGCCR 1 cut(s) 1061
EagI CGGCCG 1 cut(s) 1061
Eam1104I CTCTTC 8 cut(s) 34, 124, 293, 364, 369, 490, 522, 989
EarI CTCTTC 8 cut(s) 34, 124, 293, 364, 369, 490, 522, 989
EclXI CGGCCG 1 cut(s) 1061
Eco130I CCWWGG 1 cut(s) 1080
Eco147I AGGCCT 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 820, 1072
Eco52I CGGCCG 1 cut(s) 1061
Eco57I CTGAAG 1 cut(s) 1196
Eco88I CYCGRG 1 cut(s) 619
Eco91I GGTNACC 1 cut(s) 1084
EcoO65I GGTNACC 1 cut(s) 1084
EcoRI GAATTC 1 cut(s) 628
EcoRII CCWGG 1 cut(s) 611
EcoT14I CCWWGG 1 cut(s) 1080
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 1080
FaeI CATG 1 cut(s) 917
FaqI GGGAC 2 cut(s) 955, 998
FatI CATG 1 cut(s) 913
Fnu4HI GCNGC 6 cut(s) 536, 539, 948, 951, 1148, 1220
FokI GGATG 2 cut(s) 136, 1055
Fsp4HI GCNGC 6 cut(s) 536, 539, 948, 951, 1148, 1220
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 567, 609
GluI GCNGC 6 cut(s) 536, 539, 948, 951, 1148, 1220
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 3 cut(s) 557, 1063, 1105
HapII CCGG 5 cut(s) 605, 633, 645, 657, 1089
HhaI GCGC 2 cut(s) 568, 610
Hin1I GRCGYC 2 cut(s) 584, 608
Hin1II CATG 1 cut(s) 917
Hin6I GCGC 2 cut(s) 566, 608
HinP1I GCGC 2 cut(s) 566, 608
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 5 cut(s) 5, 218, 683, 833, 1057
HpaII CCGG 5 cut(s) 605, 633, 645, 657, 1089
HphI GGTGA 3 cut(s) 863, 980, 1096
Hpy166II GTNNAC 2 cut(s) 466, 820
Hpy188I TCNGA 3 cut(s) 34, 553, 1167
Hpy188III TCNNGA 4 cut(s) 234, 494, 1214, 1227
Hpy8I GTNNAC 2 cut(s) 466, 820
Hpy99I CGWCG 6 cut(s) 38, 506, 509, 565, 589, 1129
HpyCH4III ACNGT 2 cut(s) 673, 878
HpyCH4IV ACGT 1 cut(s) 584
HpyCH4V TGCA 2 cut(s) 779, 1150
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 554, 563, 605, 1144
HpySE526I ACGT 1 cut(s) 584
Hsp92I GRCGYC 2 cut(s) 584, 608
Hsp92II CATG 1 cut(s) 917
HspAI GCGC 2 cut(s) 566, 608
KasI GGCGCC 1 cut(s) 607
Kzo9I GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
LmnI GCTCC 1 cut(s) 1216
Lsp1109I GCAGC 1 cut(s) 1134
LweI GCATC 1 cut(s) 1171
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 3 cut(s) 580, 803, 1084
MalI GATC 6 cut(s) 48, 238, 723, 753, 771, 1169
MbiI CCGCTC 1 cut(s) 1219
MboI GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
MflI RGATCY 1 cut(s) 236
MluCI AATT 6 cut(s) 160, 406, 449, 628, 763, 871
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 2 cut(s) 212, 692
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 2 cut(s) 981, 1035
MspI CCGG 5 cut(s) 605, 633, 645, 657, 1089
MspR9I CCNGG 2 cut(s) 613, 1090
MvaI CCWGG 1 cut(s) 613
MwoI GCNNNNNNNGC 5 cut(s) 23, 554, 563, 605, 1144
NarI GGCGCC 1 cut(s) 608
NciI CCSGG 1 cut(s) 1090
NdeII GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 917
NlaIV GGNNCC 3 cut(s) 609, 643, 655
NmeAIII GCCGAG 1 cut(s) 1089
NmuCI GTSAC 2 cut(s) 580, 1084
PceI AGGCCT 1 cut(s) 557
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 501, 557
PfeI GAWTC 2 cut(s) 833, 1057
PflMI CCANNNNNTGG 1 cut(s) 829
PkrI GCNGC 6 cut(s) 537, 540, 949, 952, 1149, 1221
PleI GAGTC 2 cut(s) 212, 691
PluTI GGCGCC 1 cut(s) 611
PpsI GAGTC 2 cut(s) 212, 691
Psp6I CCWGG 1 cut(s) 611
PspEI GGTNACC 1 cut(s) 1084
PspGI CCWGG 1 cut(s) 611
PspN4I GGNNCC 3 cut(s) 609, 643, 655
PspPI GGNCC 3 cut(s) 820, 1072, 1103
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 1 cut(s) 1181
RsaNI GTAC 1 cut(s) 1180
SaqAI TTAA 2 cut(s) 981, 1035
SatI GCNGC 6 cut(s) 536, 539, 948, 951, 1148, 1220
Sau3AI GATC 6 cut(s) 46, 236, 721, 751, 769, 1167
Sau96I GGNCC 3 cut(s) 820, 1072, 1103
SchI GAGTC 2 cut(s) 212, 692
ScrFI CCNGG 2 cut(s) 613, 1090
SfaNI GCATC 1 cut(s) 1171
SfoI GGCGCC 1 cut(s) 609
SinI GGWCC 2 cut(s) 820, 1072
SmlI CTYRAG 2 cut(s) 232, 1028
SmoI CTYRAG 2 cut(s) 232, 1028
Sse9I AATT 6 cut(s) 160, 406, 449, 628, 763, 871
SseBI AGGCCT 1 cut(s) 557
SsiI CCGC 5 cut(s) 535, 538, 947, 950, 1219
SspDI GGCGCC 1 cut(s) 607
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 2 cut(s) 611, 1088
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 2 cut(s) 673, 878
TaiI ACGT 1 cut(s) 587
TaqI TCGA 3 cut(s) 495, 860, 1124
TasI AATT 6 cut(s) 160, 406, 449, 628, 763, 871
TatI WGTACW 1 cut(s) 1179
TauI GCSGC 5 cut(s) 538, 541, 950, 953, 1222
TfiI GAWTC 2 cut(s) 833, 1057
Tru1I TTAA 2 cut(s) 981, 1035
Tru9I TTAA 2 cut(s) 981, 1035
TscAI CASTG 1 cut(s) 883
TseFI GTSAC 2 cut(s) 580, 1084
TseI GCWGC 1 cut(s) 1147
Tsp45I GTSAC 2 cut(s) 580, 1084
TspDTI ATGAA 4 cut(s) 138, 744, 1067, 1205
TspGWI ACGGA 1 cut(s) 568
TspRI CASTG 1 cut(s) 883
Van91I CCANNNNNTGG 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 820, 1072
XapI RAATTY 2 cut(s) 628, 763
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.