FvH4_4g07150

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016126
Physical Location & Seq
Forward (+)
6560351 .. 6563562
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07150.t1

Sequence Viewer

Length: 1119 bp
ATGGACTCTCTGCTAGCAAGCTACGCCTCTTCCGACGAAGAAGAAGACCAAAAACCCCAACTTCAACCACCCCCAAACCCTTCATCTCCTTCTTCTTCTTCTCTCCCCAAACCCAAACCGTCATCCTTGTTCAATTCTCTTCCTCCTCCAAAACCCACATCAAGATCCTCCCTTTTCTCTTCTCTCCCCAAACCTAAACTCGAACCCCAAGATATCAAACCCAAAATCCCTAACAAACCCATCTCCAATTCCTCTTCTTCTCTCCAAATCCCCAAAAGGGTCGTCCAGTTCAAGCCTCCCATCTTCGCCAATTCGACCCATCTCGACGAAGACGACGACGACGACAATGAGGAAGAAGAGCGCCGGCGGCGGAAAGCGTCGGAGGCCTCGTCGGCGCAGCCTCAGTCCGTGACGTCGTTCTTGTCGGCTATTCCGGCGCCGAGGAACTCCGCAACGCTGGGTGTGAGTTCCGGTTTGGGTTCTGGTGCAGGCTCCGGGAGAAGAGCCATTGTTGAAACGGAGTCGTCGTTTAAAGTGGAGAGCGATGTGGGGTTGGATCAGAATGAGAATGTAGCTAGTTATGATAATTCGAATTTCGATCCGAATGTGGAGAGCTATGAAGGTTATGGTGGTTATGAGAGTTACCAATATGGTGTTGATCATAATGTGGATTCGGGGGTTCAGTTACAAACTGGTGTTAGTGGCAGTGATGCTTTGAGCTATGGTGGTTATGGTGGGTATAGTGGTTATGCTGGAAAGCATGGGAATGAATGGGCAGATGGGTCGCAAGCTGCGGTGATGGGTATGTCAGGTGATGCTGGTGTTAGAGTGAGTGGGAAGAGAAGGAGGAATGATGTTCCGACTGAAATACTTGAGGTTAAGCAGGATGAGTTGATGAAGAATCGGCCGAGGGAGGACCAAGCCAAGGTCACCGGGATTGCTTTTGGGCCTTCTTACCAGCCTGTCTCGGCAAAGGGGAAGCCAACGAAGCTGCATAAGAGGAAGCATCAGATTGGTTCTCTGTACTTTGACATGAAGCAGAAGGAGATGGAACTTCAAGAGCGCCGTTCAAGAGGCTTCCTTACAAAAGCTGAAACACAAGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

373

Amino Acids

40.44

Weight (kDa)

6.06

Isoelectric Point (pI)

61.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRCC PF10253 82 - 372 2.3e-09 Mitotic checkpoint regulator, MAD2B-interacting
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 436
AciI CCGC 4 cut(s) 367, 370, 450, 794
AclWI GGATC 3 cut(s) 159, 564, 593
AcoI YGGCCR 1 cut(s) 905
AcsI RAATTY 1 cut(s) 592
AcyI GRCGYC 2 cut(s) 413, 437
AfaI GTAC 1 cut(s) 1025
AfiI CCNNNNNNNGG 2 cut(s) 277, 925
AgsI TTSAA 6 cut(s) 65, 133, 292, 515, 1058, 1071
AluBI AGCT 7 cut(s) 21, 575, 615, 720, 791, 991, 1091
AluI AGCT 7 cut(s) 21, 575, 615, 720, 791, 991, 1091
Alw26I GTCTC 1 cut(s) 970
AlwI GGATC 3 cut(s) 159, 564, 593
AoxI GGCC 3 cut(s) 384, 905, 947
ApeKI GCWGC 3 cut(s) 397, 791, 991
ApoI RAATTY 1 cut(s) 592
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AspLEI GCGC 4 cut(s) 363, 397, 439, 1065
AspS9I GGNCC 2 cut(s) 916, 947
AsuC2I CCSGG 2 cut(s) 496, 934
AsuHPI GGTGA 3 cut(s) 808, 824, 922
AsuII TTCGAA 1 cut(s) 590
AsuNHI GCTAGC 1 cut(s) 13
AvaII GGWCC 1 cut(s) 916
BanI GGYRCC 1 cut(s) 436
BbsI GAAGAC 2 cut(s) 51, 336
BbvI GCAGC 3 cut(s) 409, 778, 978
BccI CCATC 7 cut(s) 248, 308, 327, 773, 793, 1042, 1107
BceAI ACGGC 1 cut(s) 1050
BcgI CGANNNNNNTGC 2 cut(s) 765, 799
BclI TGATCA 1 cut(s) 658
BcnI CCSGG 2 cut(s) 496, 934
BcoDI GTCTC 1 cut(s) 970
BfaI CTAG 2 cut(s) 14, 576
BfoI RGCGCY 3 cut(s) 364, 440, 1066
BglI GCCNNNNNGGC 1 cut(s) 392
BisI GCNGC 4 cut(s) 368, 398, 792, 992
BlsI GCNGC 4 cut(s) 369, 399, 793, 993
Bme1390I CCNGG 2 cut(s) 496, 934
Bme18I GGWCC 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 916, 947
BmiI GGNNCC 2 cut(s) 438, 493
BmrFI CCNGG 2 cut(s) 496, 934
BmsI GCATC 3 cut(s) 700, 805, 1015
BmtI GCTAGC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 51, 336
Bpu14I TTCGAA 1 cut(s) 590
BpuEI CTTGAG 1 cut(s) 893
BpuMI CCSGG 2 cut(s) 496, 934
BsaHI GRCGYC 2 cut(s) 413, 437
BsaJI CCNNGG 3 cut(s) 440, 908, 924
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 4 cut(s) 374, 404, 512, 542
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 925
Bse118I RCCGGY 1 cut(s) 363
Bse1I ACTGG 2 cut(s) 286, 697
BseDI CCNNGG 3 cut(s) 440, 908, 924
BseGI GGATG 3 cut(s) 122, 892, 1118
BseLI CCNNNNNNNGG 2 cut(s) 277, 925
BseMII CTCAG 1 cut(s) 416
BseNI ACTGG 2 cut(s) 286, 697
BseRI GAGGAG 1 cut(s) 135
BseX3I CGGCCG 1 cut(s) 905
BseXI GCAGC 3 cut(s) 409, 778, 978
BseYI CCCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 908
BshFI GGCC 3 cut(s) 386, 907, 949
BshNI GGYRCC 1 cut(s) 436
BsiEI CGRYCG 1 cut(s) 908
BsiSI CCGG 5 cut(s) 364, 434, 471, 495, 933
BslI CCNNNNNNNGG 2 cut(s) 277, 925
BsmAI GTCTC 1 cut(s) 970
BsnI GGCC 3 cut(s) 386, 907, 949
Bsp119I TTCGAA 1 cut(s) 590
Bsp143I GATC 4 cut(s) 164, 556, 598, 658
BspACI CCGC 4 cut(s) 367, 370, 450, 794
BspANI GGCC 3 cut(s) 386, 907, 949
BspCNI CTCAG 1 cut(s) 415
BspLI GGNNCC 2 cut(s) 438, 493
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 159, 564, 593
BspQI GCTCTTC 2 cut(s) 351, 496
BspT104I TTCGAA 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 363
BsrI ACTGG 2 cut(s) 286, 697
BssAI RCCGGY 1 cut(s) 363
BssECI CCNNGG 3 cut(s) 440, 908, 924
BssMI GATC 4 cut(s) 164, 556, 598, 658
BssNI GRCGYC 2 cut(s) 413, 437
BssT1I CCWWGG 1 cut(s) 924
Bst4CI ACNGT 1 cut(s) 120
Bst6I CTCTTC 7 cut(s) 34, 144, 184, 259, 351, 496, 833
BstACI GRCGYC 2 cut(s) 413, 437
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 5 cut(s) 15, 19, 365, 490, 789
BstDEI CTNAG 1 cut(s) 402
BstEII GGTNACC 1 cut(s) 928
BstF5I GGATG 3 cut(s) 122, 892, 1118
BstH2I RGCGCY 3 cut(s) 364, 440, 1066
BstHHI GCGC 4 cut(s) 363, 397, 439, 1065
BstKTI GATC 4 cut(s) 167, 559, 601, 661
BstMAI GTCTC 1 cut(s) 970
BstMBI GATC 4 cut(s) 164, 556, 598, 658
BstMCI CGRYCG 1 cut(s) 908
BstMWI GCNNNNNNNGC 6 cut(s) 23, 367, 383, 392, 434, 988
BstPI GGTNACC 1 cut(s) 928
BstSCI CCNGG 2 cut(s) 494, 932
BstV1I GCAGC 3 cut(s) 409, 778, 978
BstV2I GAAGAC 2 cut(s) 51, 336
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
BstZI CGGCCG 1 cut(s) 905
BsuRI GGCC 3 cut(s) 386, 907, 949
BtgZI GCGATG 1 cut(s) 558
BtsCI GGATG 3 cut(s) 122, 892, 1118
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 712
Cac8I GCNNGC 5 cut(s) 15, 19, 365, 490, 789
CfoI GCGC 4 cut(s) 363, 397, 439, 1065
Cfr10I RCCGGY 1 cut(s) 363
Cfr13I GGNCC 2 cut(s) 916, 947
CseI GACGC 1 cut(s) 366
Csp6I GTAC 1 cut(s) 1024
CviAII CATG 2 cut(s) 761, 1033
CviQI GTAC 1 cut(s) 1024
DdeI CTNAG 1 cut(s) 402
DinI GGCGCC 1 cut(s) 438
DpnI GATC 4 cut(s) 166, 558, 600, 660
DpnII GATC 4 cut(s) 164, 556, 598, 658
DraI TTTAAA 1 cut(s) 532
EaeI YGGCCR 1 cut(s) 905
EagI CGGCCG 1 cut(s) 905
Eam1104I CTCTTC 7 cut(s) 34, 144, 184, 259, 351, 496, 833
EarI CTCTTC 7 cut(s) 34, 144, 184, 259, 351, 496, 833
EciI GGCGGA 1 cut(s) 385
EclXI CGGCCG 1 cut(s) 905
Eco130I CCWWGG 1 cut(s) 924
Eco147I AGGCCT 1 cut(s) 386
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 916
Eco52I CGGCCG 1 cut(s) 905
Eco91I GGTNACC 1 cut(s) 928
EcoO65I GGTNACC 1 cut(s) 928
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 924
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 924
FaeI CATG 2 cut(s) 764, 1036
FatI CATG 2 cut(s) 760, 1032
FbaI TGATCA 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 368, 398, 792, 992
FokI GGATG 2 cut(s) 109, 899
Fsp4HI GCNGC 4 cut(s) 368, 398, 792, 992
FspBI CTAG 2 cut(s) 14, 576
GlaI GCGC 4 cut(s) 362, 396, 438, 1064
GluI GCNGC 4 cut(s) 368, 398, 792, 992
GsaI CCCAGC 1 cut(s) 461
HaeII RGCGCY 3 cut(s) 364, 440, 1066
HaeIII GGCC 3 cut(s) 386, 907, 949
HapII CCGG 5 cut(s) 364, 434, 471, 495, 933
HgaI GACGC 1 cut(s) 366
HhaI GCGC 4 cut(s) 363, 397, 439, 1065
Hin1I GRCGYC 2 cut(s) 413, 437
Hin1II CATG 2 cut(s) 764, 1036
Hin6I GCGC 4 cut(s) 361, 395, 437, 1063
HinP1I GCGC 4 cut(s) 361, 395, 437, 1063
HinfI GANTC 4 cut(s) 5, 521, 671, 901
HpaII CCGG 5 cut(s) 364, 434, 471, 495, 933
HphI GGTGA 3 cut(s) 808, 824, 922
Hpy188I TCNGA 6 cut(s) 34, 382, 561, 603, 861, 1011
Hpy188III TCNNGA 4 cut(s) 162, 323, 1058, 1071
Hpy99I CGWCG 9 cut(s) 38, 329, 338, 341, 344, 382, 394, 418, 529
HpyAV CCTTC 6 cut(s) 90, 99, 614, 837, 960, 1036
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 2 cut(s) 488, 994
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 367, 383, 392, 434, 988
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 413
Hsp92I GRCGYC 2 cut(s) 413, 437
Hsp92II CATG 2 cut(s) 764, 1036
HspAI GCGC 4 cut(s) 361, 395, 437, 1063
KasI GGCGCC 1 cut(s) 436
KroI GCCGGC 1 cut(s) 363
KroNI GCCGGC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 4 cut(s) 164, 556, 598, 658
LguI GCTCTTC 2 cut(s) 351, 496
LmnI GCTCC 1 cut(s) 497
Lsp1109I GCAGC 3 cut(s) 409, 778, 978
LweI GCATC 3 cut(s) 700, 805, 1015
MaeI CTAG 2 cut(s) 14, 576
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 4 cut(s) 409, 641, 684, 928
MalI GATC 4 cut(s) 166, 558, 600, 660
MboI GATC 4 cut(s) 164, 556, 598, 658
MflI RGATCY 1 cut(s) 164
MluCI AATT 5 cut(s) 133, 247, 310, 586, 592
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 4 cut(s) 57, 360, 534, 884
MreI CGCCGGCG 1 cut(s) 363
MroNI GCCGGC 1 cut(s) 363
MseI TTAA 2 cut(s) 531, 879
MslI CAYNNNNRTG 1 cut(s) 765
MspI CCGG 5 cut(s) 364, 434, 471, 495, 933
MspR9I CCNGG 2 cut(s) 496, 934
MwoI GCNNNNNNNGC 6 cut(s) 23, 367, 383, 392, 434, 988
NaeI GCCGGC 1 cut(s) 365
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 2 cut(s) 496, 934
NdeII GATC 4 cut(s) 164, 556, 598, 658
NgoMIV GCCGGC 1 cut(s) 363
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 2 cut(s) 764, 1036
NlaIV GGNNCC 2 cut(s) 438, 493
NmeAIII GCCGAG 3 cut(s) 465, 933, 947
NmuCI GTSAC 2 cut(s) 409, 928
NspV TTCGAA 1 cut(s) 590
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 2 cut(s) 351, 496
PcsI WCGNNNNNNNCGW 6 cut(s) 30, 330, 333, 339, 386, 524
PdiI GCCGGC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 671, 901
PfoI TCCNGGA 1 cut(s) 494
PkrI GCNGC 4 cut(s) 369, 399, 793, 993
PleI GAGTC 1 cut(s) 529
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 529
PspEI GGTNACC 1 cut(s) 928
PspFI CCCAGC 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 438, 493
PspPI GGNCC 2 cut(s) 916, 947
PsuI RGATCY 1 cut(s) 164
RsaI GTAC 1 cut(s) 1025
RsaNI GTAC 1 cut(s) 1024
RseI CAYNNNNRTG 1 cut(s) 765
SapI GCTCTTC 2 cut(s) 351, 496
SaqAI TTAA 2 cut(s) 531, 879
SatI GCNGC 4 cut(s) 368, 398, 792, 992
Sau3AI GATC 4 cut(s) 164, 556, 598, 658
Sau96I GGNCC 2 cut(s) 916, 947
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 496, 934
SfaNI GCATC 3 cut(s) 700, 805, 1015
SfoI GGCGCC 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 590
SgrAI CRCCGGYG 1 cut(s) 363
SinI GGWCC 1 cut(s) 916
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 5 cut(s) 133, 247, 310, 586, 592
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 4 cut(s) 367, 370, 450, 794
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 2 cut(s) 14, 576
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 2 cut(s) 494, 932
StyI CCWWGG 1 cut(s) 924
TaaI ACNGT 1 cut(s) 120
TaiI ACGT 1 cut(s) 416
TaqI TCGA 5 cut(s) 201, 314, 324, 590, 597
TasI AATT 5 cut(s) 133, 247, 310, 586, 592
TatI WGTACW 1 cut(s) 1023
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 2 cut(s) 671, 901
Tru1I TTAA 2 cut(s) 531, 879
Tru9I TTAA 2 cut(s) 531, 879
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 2 cut(s) 409, 928
TseI GCWGC 3 cut(s) 397, 791, 991
Tsp45I GTSAC 2 cut(s) 409, 928
TspDTI ATGAA 5 cut(s) 72, 633, 783, 911, 1049
TspGWI ACGGA 2 cut(s) 397, 533
TspRI CASTG 1 cut(s) 712
VpaK11BI GGWCC 1 cut(s) 916
XapI RAATTY 1 cut(s) 592
XspI CTAG 2 cut(s) 14, 576
ZraI GACGTC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.