RLG00000009275

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016126
Physical Location & Seq
Reverse (-)
50140594 .. 50143911
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009275

Sequence Viewer

Length: 1287 bp
ATGGACTCTCTGCTAGCAAACTACGCCTCTTCCGACGAAGAAGAAGATCAAAAACCCCACCACCACCAACTACAACCACCTCCAAACCCTTCTTCTTCTTCTTCTTCTTCTCTTTTCTCTTCTCTACCCAAACCCAAACCCAAACCCGAACCTTCTTCATCCTTCTTCAATTCCCTTCCTCCTCCTCCAAAACAAACCCAATCAAATCCTCCCCACCAAGAAGACCAAGACTCAAAACCCACCTCAAGATCTTCTCTTTTCTCCTCTCAACCCAAACCCAAACCCTCCTCTCTCTTCCAATCCCTTCCCAAACCAAAACTCGAACAACAAGATGAAGAAGAAGAAGACATAAAACCCTCTTCTTCTGTTTTCTCTTCTCTTCCTCCCCCCAAATCCCAAATCCCTAACAAACCCATCTCCAATTCCTCATCCTCTCCTGCCCAAATCCCCAAAAGGGTCGTCCAATTCAAGCCTCCCATCAACCCTTATTTCGCAAACTCTTCCCATCTCGAAGACGACGACGACGATGAGGAAGAAGAAGAGAGGCGGCGGCGAAAAGCCTCGGAGGCGTCGTCGGCGCAACCTTTGTCCGTGACGTCGTTCTTATCAGCTATTCCGGCGCCCAGGAACTCGGGTGTGAATTCCGGTTTAGGTTCCGGCGTAGGTTCCGGTAGGAGAGCCACTGTTGAAACAGAGTCGTCTGGTTCTAAAGTGGAGAGCGATGTGGGTTTGGACCGAAATGAAAATGTAGCAAGTTATGATGATCCCCAATCAAATTTTGATCCAAATGCAGAAGGTTATGGTGGGTATGAGAGTTACCAATATGGTGTGGACCAAAATGTGGATTCTGGGGTTAGTGGCGGTGATGGTTCGAGCTATGGTAATTACAGTGGTTATGGTGGGTATAATGGTTATACTGGAAATCATGGGAACGAATGGGTTGATGGGTCGGGGCCTGCGGCGGCAATGGGTGTGTCAGGTGATACTGGGGTTAAAGTGAGTGGGAAGAGGAGGAGGAATGATGTCCCAACTGAAATACTTGAGGTTAAGCAGGATGAGTTGATGAAGAATCGGCCGAGGGAGGACCAAGCCAAGGTGACCGGGATAGCTTTTGGGCCTTCTTACCAGCCTGTTTCAACGAAGGGGAAGCCAACAAAGCTGCATAAGAGGAAGCATCAGATTGGTTCTCTGTACTTTGATATGAAGCAGAAGGAGATGGAACTTCAGGAGCGGCGTTCAAGAGGCTTCCTTACCAAAGCTGAAACACAAGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

429

Amino Acids

46.61

Weight (kDa)

5.64

Isoelectric Point (pI)

65.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 599
AccB1I GGYRCC 1 cut(s) 619
AccB7I CCANNNNNTGG 1 cut(s) 841
AccBSI CCGCTC 1 cut(s) 1231
AciI CCGC 6 cut(s) 547, 550, 861, 959, 962, 1231
AclWI GGATC 2 cut(s) 758, 776
AcoI YGGCCR 1 cut(s) 1073
AcsI RAATTY 2 cut(s) 640, 775
AcuI CTGAAG 1 cut(s) 1208
AcyI GRCGYC 3 cut(s) 569, 596, 620
AfaI GTAC 1 cut(s) 1193
AfiI CCNNNNNNNGG 3 cut(s) 454, 841, 1093
AgsI TTSAA 5 cut(s) 169, 469, 689, 1137, 1239
AjnI CCWGG 1 cut(s) 623
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 5 cut(s) 611, 876, 1109, 1159, 1259
AluI AGCT 5 cut(s) 611, 876, 1109, 1159, 1259
AlwI GGATC 2 cut(s) 758, 776
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 3 cut(s) 953, 1073, 1115
ApeKI GCWGC 1 cut(s) 1159
ApoI RAATTY 2 cut(s) 640, 775
ArsI GACNNNNNNTTYG 2 cut(s) 444, 476
AspLEI GCGC 2 cut(s) 580, 622
AspS9I GGNCC 5 cut(s) 733, 832, 953, 1084, 1115
AsuC2I CCSGG 1 cut(s) 1102
AsuHPI GGTGA 3 cut(s) 875, 992, 1108
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 3 cut(s) 733, 832, 1084
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BanI GGYRCC 1 cut(s) 619
BbsI GAAGAC 3 cut(s) 228, 351, 519
BbvI GCAGC 1 cut(s) 1146
BccI CCATC 7 cut(s) 422, 485, 513, 860, 938, 1210, 1275
BciT130I CCWGG 1 cut(s) 625
BcnI CCSGG 1 cut(s) 1102
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 623
BglI GCCNNNNNGGC 1 cut(s) 566
BglII AGATCT 1 cut(s) 248
BisI GCNGC 6 cut(s) 548, 551, 960, 963, 1160, 1232
BlsI GCNGC 6 cut(s) 549, 552, 961, 964, 1161, 1233
Bme1390I CCNGG 2 cut(s) 625, 1102
Bme18I GGWCC 3 cut(s) 733, 832, 1084
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 5 cut(s) 733, 832, 953, 1084, 1115
BmiI GGNNCC 4 cut(s) 621, 655, 667, 954
BmrFI CCNGG 2 cut(s) 625, 1102
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 1 cut(s) 1183
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 996
BpiI GAAGAC 3 cut(s) 228, 351, 519
BpuEI CTTGAG 2 cut(s) 229, 1061
BpuMI CCSGG 1 cut(s) 1102
BsaHI GRCGYC 3 cut(s) 569, 596, 620
BsaJI CCNNGG 4 cut(s) 561, 623, 1076, 1092
BsaWI WCCGGW 2 cut(s) 644, 668
BsaXI ACNNNNNCTCC 2 cut(s) 557, 587
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 841, 1093
Bse1I ACTGG 2 cut(s) 922, 991
Bse3DI GCAATG 1 cut(s) 972
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 4 cut(s) 561, 623, 1076, 1092
BseGI GGATG 4 cut(s) 158, 428, 1060, 1286
BseLI CCNNNNNNNGG 3 cut(s) 454, 841, 1093
BseMI GCAATG 1 cut(s) 972
BseNI ACTGG 2 cut(s) 922, 991
BseRI GAGGAG 6 cut(s) 171, 174, 253, 277, 1024, 1027
BseX3I CGGCCG 1 cut(s) 1073
BseXI GCAGC 1 cut(s) 1146
Bsh1285I CGRYCG 1 cut(s) 1076
BshFI GGCC 3 cut(s) 955, 1075, 1117
BshNI GGYRCC 1 cut(s) 619
BsiEI CGRYCG 1 cut(s) 1076
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 5 cut(s) 617, 645, 657, 669, 1101
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 3 cut(s) 454, 841, 1093
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 3 cut(s) 955, 1075, 1117
BsoBI CYCGRG 1 cut(s) 631
Bsp143I GATC 4 cut(s) 46, 248, 763, 781
BspACI CCGC 6 cut(s) 547, 550, 861, 959, 962, 1231
BspANI GGCC 3 cut(s) 955, 1075, 1117
BspLI GGNNCC 4 cut(s) 621, 655, 667, 954
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 758, 776
BspT107I GGYRCC 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 1231
BsrDI GCAATG 1 cut(s) 972
BsrI ACTGG 2 cut(s) 922, 991
BssECI CCNNGG 4 cut(s) 561, 623, 1076, 1092
BssMI GATC 4 cut(s) 46, 248, 763, 781
BssNI GRCGYC 3 cut(s) 569, 596, 620
BssT1I CCWWGG 1 cut(s) 1092
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 2 cut(s) 685, 890
Bst6I CTCTTC 9 cut(s) 34, 124, 299, 364, 379, 384, 505, 534, 1001
BstACI GRCGYC 3 cut(s) 569, 596, 620
BstC8I GCNNGC 2 cut(s) 15, 957
BstEII GGTNACC 1 cut(s) 1096
BstF5I GGATG 4 cut(s) 158, 428, 1060, 1286
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 2 cut(s) 580, 622
BstKTI GATC 4 cut(s) 49, 251, 766, 784
BstMBI GATC 4 cut(s) 46, 248, 763, 781
BstMCI CGRYCG 1 cut(s) 1076
BstMWI GCNNNNNNNGC 5 cut(s) 23, 566, 575, 617, 1156
BstNI CCWGG 1 cut(s) 625
BstPI GGTNACC 1 cut(s) 1096
BstSCI CCNGG 2 cut(s) 623, 1100
BstV1I GCAGC 1 cut(s) 1146
BstV2I GAAGAC 3 cut(s) 228, 351, 519
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BstZI CGGCCG 1 cut(s) 1073
BsuRI GGCC 3 cut(s) 955, 1075, 1117
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 4 cut(s) 158, 428, 1060, 1286
BtsIMutI CAGTG 2 cut(s) 681, 895
Cac8I GCNNGC 2 cut(s) 15, 957
CfoI GCGC 2 cut(s) 580, 622
Cfr13I GGNCC 5 cut(s) 733, 832, 953, 1084, 1115
CseI GACGC 1 cut(s) 558
Csp6I GTAC 1 cut(s) 1192
CviAII CATG 1 cut(s) 926
CviQI GTAC 1 cut(s) 1192
DinI GGCGCC 1 cut(s) 621
DpnI GATC 4 cut(s) 48, 250, 765, 783
DpnII GATC 4 cut(s) 46, 248, 763, 781
EaeI YGGCCR 1 cut(s) 1073
EagI CGGCCG 1 cut(s) 1073
Eam1104I CTCTTC 9 cut(s) 34, 124, 299, 364, 379, 384, 505, 534, 1001
EarI CTCTTC 9 cut(s) 34, 124, 299, 364, 379, 384, 505, 534, 1001
EclXI CGGCCG 1 cut(s) 1073
Eco130I CCWWGG 1 cut(s) 1092
Eco47I GGWCC 3 cut(s) 733, 832, 1084
Eco52I CGGCCG 1 cut(s) 1073
Eco57I CTGAAG 1 cut(s) 1208
Eco88I CYCGRG 1 cut(s) 631
Eco91I GGTNACC 1 cut(s) 1096
EcoO109I RGGNCCY 1 cut(s) 953
EcoO65I GGTNACC 1 cut(s) 1096
EcoRI GAATTC 1 cut(s) 640
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 1092
EgeI GGCGCC 1 cut(s) 621
EheI GGCGCC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 1092
FaeI CATG 1 cut(s) 929
FaqI GGGAC 1 cut(s) 1010
FatI CATG 1 cut(s) 925
Fnu4HI GCNGC 6 cut(s) 548, 551, 960, 963, 1160, 1232
FokI GGATG 3 cut(s) 145, 415, 1067
Fsp4HI GCNGC 6 cut(s) 548, 551, 960, 963, 1160, 1232
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 579, 621
GluI GCNGC 6 cut(s) 548, 551, 960, 963, 1160, 1232
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 3 cut(s) 955, 1075, 1117
HapII CCGG 5 cut(s) 617, 645, 657, 669, 1101
HgaI GACGC 1 cut(s) 558
HhaI GCGC 2 cut(s) 580, 622
Hin1I GRCGYC 3 cut(s) 569, 596, 620
Hin1II CATG 1 cut(s) 929
Hin6I GCGC 2 cut(s) 578, 620
HinP1I GCGC 2 cut(s) 578, 620
HinfI GANTC 5 cut(s) 5, 230, 695, 845, 1069
HpaII CCGG 5 cut(s) 617, 645, 657, 669, 1101
HphI GGTGA 3 cut(s) 875, 992, 1108
Hpy166II GTNNAC 1 cut(s) 832
Hpy188I TCNGA 3 cut(s) 34, 565, 1179
Hpy188III TCNNGA 4 cut(s) 246, 509, 1226, 1239
Hpy8I GTNNAC 1 cut(s) 832
Hpy99I CGWCG 7 cut(s) 38, 521, 524, 527, 574, 577, 601
HpyAV CCTTC 9 cut(s) 99, 162, 172, 185, 314, 788, 1128, 1135, 1204
HpyCH4III ACNGT 2 cut(s) 685, 890
HpyCH4IV ACGT 1 cut(s) 596
HpyCH4V TGCA 2 cut(s) 791, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 566, 575, 617, 1156
HpySE526I ACGT 1 cut(s) 596
Hsp92I GRCGYC 3 cut(s) 569, 596, 620
Hsp92II CATG 1 cut(s) 929
HspAI GCGC 2 cut(s) 578, 620
KasI GGCGCC 1 cut(s) 619
Kzo9I GATC 4 cut(s) 46, 248, 763, 781
LmnI GCTCC 1 cut(s) 1228
Lsp1109I GCAGC 1 cut(s) 1146
LweI GCATC 1 cut(s) 1183
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 596
MaeIII GTNAC 3 cut(s) 592, 815, 1096
MalI GATC 4 cut(s) 48, 250, 765, 783
MbiI CCGCTC 1 cut(s) 1231
MboI GATC 4 cut(s) 46, 248, 763, 781
MflI RGATCY 1 cut(s) 248
MluCI AATT 6 cut(s) 169, 421, 464, 640, 775, 883
Mly113I GGCGCC 1 cut(s) 620
MlyI GAGTC 2 cut(s) 224, 704
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 2 cut(s) 993, 1047
MspI CCGG 5 cut(s) 617, 645, 657, 669, 1101
MspR9I CCNGG 2 cut(s) 625, 1102
MvaI CCWGG 1 cut(s) 625
MwoI GCNNNNNNNGC 5 cut(s) 23, 566, 575, 617, 1156
NarI GGCGCC 1 cut(s) 620
NciI CCSGG 1 cut(s) 1102
NdeII GATC 4 cut(s) 46, 248, 763, 781
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 929
NlaIV GGNNCC 4 cut(s) 621, 655, 667, 954
NmeAIII GCCGAG 1 cut(s) 1101
NmuCI GTSAC 2 cut(s) 592, 1096
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 516, 522, 569
PfeI GAWTC 2 cut(s) 845, 1069
PflMI CCANNNNNTGG 1 cut(s) 841
PkrI GCNGC 6 cut(s) 549, 552, 961, 964, 1161, 1233
PleI GAGTC 2 cut(s) 224, 703
PluTI GGCGCC 1 cut(s) 623
PpsI GAGTC 2 cut(s) 224, 703
Psp6I CCWGG 1 cut(s) 623
PspEI GGTNACC 1 cut(s) 1096
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 4 cut(s) 621, 655, 667, 954
PspPI GGNCC 5 cut(s) 733, 832, 953, 1084, 1115
PsuI RGATCY 1 cut(s) 248
RsaI GTAC 1 cut(s) 1193
RsaNI GTAC 1 cut(s) 1192
SaqAI TTAA 2 cut(s) 993, 1047
SatI GCNGC 6 cut(s) 548, 551, 960, 963, 1160, 1232
Sau3AI GATC 4 cut(s) 46, 248, 763, 781
Sau96I GGNCC 5 cut(s) 733, 832, 953, 1084, 1115
SchI GAGTC 2 cut(s) 224, 704
ScrFI CCNGG 2 cut(s) 625, 1102
SfaNI GCATC 1 cut(s) 1183
SfoI GGCGCC 1 cut(s) 621
SinI GGWCC 3 cut(s) 733, 832, 1084
SmlI CTYRAG 2 cut(s) 244, 1040
SmoI CTYRAG 2 cut(s) 244, 1040
Sse9I AATT 6 cut(s) 169, 421, 464, 640, 775, 883
SsiI CCGC 6 cut(s) 547, 550, 861, 959, 962, 1231
SspDI GGCGCC 1 cut(s) 619
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 623, 1100
StyI CCWWGG 1 cut(s) 1092
TaaI ACNGT 2 cut(s) 685, 890
TaiI ACGT 1 cut(s) 599
TaqI TCGA 3 cut(s) 321, 510, 872
TaqII GACCGA 1 cut(s) 750
TasI AATT 6 cut(s) 169, 421, 464, 640, 775, 883
TatI WGTACW 1 cut(s) 1191
TauI GCSGC 5 cut(s) 550, 553, 962, 965, 1234
TfiI GAWTC 2 cut(s) 845, 1069
Tru1I TTAA 2 cut(s) 993, 1047
Tru9I TTAA 2 cut(s) 993, 1047
TscAI CASTG 2 cut(s) 688, 895
TseFI GTSAC 2 cut(s) 592, 1096
TseI GCWGC 1 cut(s) 1159
Tsp45I GTSAC 2 cut(s) 592, 1096
TspDTI ATGAA 5 cut(s) 147, 348, 756, 1079, 1217
TspGWI ACGGA 1 cut(s) 580
TspRI CASTG 2 cut(s) 688, 895
Van91I CCANNNNNTGG 1 cut(s) 841
VpaK11BI GGWCC 3 cut(s) 733, 832, 1084
XapI RAATTY 2 cut(s) 640, 775
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.