Rh4CG102300

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016126
Physical Location & Seq
Forward (+)
18842499 .. 18846907
4409 bp
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UTR
Exon/CDS
Intron
Rh4CG102300.1

Sequence Viewer

Length: 1254 bp
ATGGACTCTCTGCTAGCAAACTACGCCTCTTCCGACGAAGAAGAAGATCAAAAACCCCACCACCACCAACTACAACCACCTCCAAACGCTTCTTCTTCTCTTTTCTCTTCTCTACCCAAACCCGAACCTTTATCCTTCTTCAATTCCCTTCCTCCTCCAAAACAAACCCAATCAAATCCTCCCCACCAAGAAGACCAAGACTCAAAACCCACCTCAAGATCTTCTCTTTTCTCCTCTCTACCCAAACCCAAACCCTCCTCTCTCTTCCAATCCCTTCCCAAACCAAAACTTGAACCACAACAAGATGAAGAAGAAGAAGAAGACATAAAACCCCCTTCTTCTGTTTTCTCTTCTCTTCCTCCCCCCAAATCCCAAATCCCTAACAAACCCATCTCCAATTCCTCATCTTCTCCTGCCCAAATCCCCAAAAGGGTCGTCCAATTCAAGCCTCCCGTCAACCCTTATTTCGCGAACTCTTCCCATCTCGAAGACGACGACGACGATGAGGAAGAAGAAGAGAGGCGGCGGCGAAAAGCCTCGGAGGCCTCGTCGGCGCAACCTTTGTCCGTGACGTCGTTCTTATCAGCTATTCCGGCGCCCAGGAACTCGGGTGTGAATTCCGGTTTAGGTTCCGGTGTAGGTTCCGGTAGGAGAGCCACCGTTGAAACTGAGTCGTTTGGTTCTAAAGTGGAGAGCGATGTGGGTTTGGATCGAAATGAAAATGTAGCAAGTTATGATGATCCCCAATCAAATTTTGATCCAAATGCAGAAGGTTATGGTGGGTATGAGAGTTACCAATATGGTGTTGACCACAATGTGGATTCTGGGGTTAGTGGCGGTGATGGTTCGAGCTATGGTAGTTACAGTGGCTATGGTGGGTATAATGGTTATGCTGGAAATCATGGGAACGAATGGGCTGATGGGTCGGGGACTGCGGCAGCAATGGGTGATACTGGGGTTAAAGTGAGTGGGAAGAGGAGGAGGAATGATGTCCCAACTGAAATACTTGAGGTTAAGCAGGATGAGTTGATGAAGAATCGGCCGAGGGAGGACCAAGCCAAGGTGACCGGGATAGCTTTTGGGCCTTCTTACCAGCCTGTTTCGACGAAGGGGAAGCCGACAAAGCTGCATAAGAGGAAGCATCAGATTGGTTCTCTGTACTTTGATATGAAGCAGAAGGAGATGGAACTTCAGGAGCGGCGTTCAAGAGGCTTCCTTACAAAAGCTGAAACACAAGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

417

Amino Acids

45.58

Weight (kDa)

5.44

Isoelectric Point (pI)

65.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRCC PF10253 185 - 417 2.3e-06 Mitotic checkpoint regulator, MAD2B-interacting
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 817
AccBSI CCGCTC 1 cut(s) 1198
AccII CGCG 1 cut(s) 470
AciI CCGC 5 cut(s) 523, 526, 837, 935, 1198
AclWI GGATC 3 cut(s) 717, 734, 752
AcoI YGGCCR 1 cut(s) 1040
AcsI RAATTY 2 cut(s) 616, 751
AcuI CTGAAG 1 cut(s) 1175
AcyI GRCGYC 2 cut(s) 572, 596
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 1 cut(s) 1160
AfiI CCNNNNNNNGG 3 cut(s) 430, 817, 1060
AgsI TTSAA 5 cut(s) 142, 293, 445, 665, 1206
AjnI CCWGG 1 cut(s) 599
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 5 cut(s) 587, 852, 1076, 1126, 1226
AluI AGCT 5 cut(s) 587, 852, 1076, 1126, 1226
AlwI GGATC 3 cut(s) 717, 734, 752
Ama87I CYCGRG 1 cut(s) 607
AoxI GGCC 3 cut(s) 543, 1040, 1082
ApeKI GCWGC 2 cut(s) 938, 1126
ApoI RAATTY 2 cut(s) 616, 751
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AspLEI GCGC 2 cut(s) 556, 598
AspS9I GGNCC 2 cut(s) 1051, 1082
AsuC2I CCSGG 1 cut(s) 1069
AsuHPI GGTGA 3 cut(s) 851, 959, 1075
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 1 cut(s) 1051
BaeI ACNNNNGTAYC 2 cut(s) 942, 975
BanI GGYRCC 1 cut(s) 595
BbsI GAAGAC 3 cut(s) 198, 327, 495
BbvI GCAGC 2 cut(s) 950, 1113
BccI CCATC 6 cut(s) 398, 489, 836, 914, 1177, 1242
BcgI CGANNNNNNTGC 2 cut(s) 1108, 1142
BciT130I CCWGG 1 cut(s) 601
BcnI CCSGG 1 cut(s) 1069
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 599
BglI GCCNNNNNGGC 2 cut(s) 542, 551
BglII AGATCT 1 cut(s) 218
BisI GCNGC 6 cut(s) 524, 527, 936, 939, 1127, 1199
BlsI GCNGC 6 cut(s) 525, 528, 937, 940, 1128, 1200
Bme1390I CCNGG 2 cut(s) 601, 1069
Bme18I GGWCC 1 cut(s) 1051
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 2 cut(s) 1051, 1082
BmiI GGNNCC 3 cut(s) 597, 631, 643
BmrFI CCNGG 2 cut(s) 601, 1069
BmrI ACTGGG 1 cut(s) 963
BmsI GCATC 1 cut(s) 1150
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 963
BpiI GAAGAC 3 cut(s) 198, 327, 495
BpuEI CTTGAG 2 cut(s) 199, 1028
BpuMI CCSGG 1 cut(s) 1069
BsaHI GRCGYC 2 cut(s) 572, 596
BsaJI CCNNGG 4 cut(s) 537, 599, 1043, 1059
BsaWI WCCGGW 3 cut(s) 620, 632, 644
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 3 cut(s) 430, 817, 1060
Bse1I ACTGG 1 cut(s) 958
Bse3DI GCAATG 1 cut(s) 948
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 4 cut(s) 537, 599, 1043, 1059
BseGI GGATG 2 cut(s) 1027, 1253
BseLI CCNNNNNNNGG 3 cut(s) 430, 817, 1060
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 1 cut(s) 660
BseNI ACTGG 1 cut(s) 958
BseRI GAGGAG 5 cut(s) 144, 223, 247, 991, 994
BseX3I CGGCCG 1 cut(s) 1040
BseXI GCAGC 2 cut(s) 950, 1113
Bsh1236I CGCG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 1043
BshFI GGCC 3 cut(s) 545, 1042, 1084
BshNI GGYRCC 1 cut(s) 595
BsiEI CGRYCG 1 cut(s) 1043
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 5 cut(s) 593, 621, 633, 645, 1068
BslFI GGGAC 2 cut(s) 943, 977
BslI CCNNNNNNNGG 3 cut(s) 430, 817, 1060
BsmFI GGGAC 2 cut(s) 943, 977
BsnI GGCC 3 cut(s) 545, 1042, 1084
BsoBI CYCGRG 1 cut(s) 607
Bsp143I GATC 5 cut(s) 46, 218, 709, 739, 757
Bsp68I TCGCGA 1 cut(s) 470
BspACI CCGC 5 cut(s) 523, 526, 837, 935, 1198
BspANI GGCC 3 cut(s) 545, 1042, 1084
BspCNI CTCAG 1 cut(s) 661
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 3 cut(s) 597, 631, 643
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 717, 734, 752
BspT107I GGYRCC 1 cut(s) 595
BsrBI CCGCTC 1 cut(s) 1198
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 1 cut(s) 958
BssECI CCNNGG 4 cut(s) 537, 599, 1043, 1059
BssMI GATC 5 cut(s) 46, 218, 709, 739, 757
BssNI GRCGYC 2 cut(s) 572, 596
BssT1I CCWWGG 1 cut(s) 1059
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 2 cut(s) 661, 866
Bst6I CTCTTC 8 cut(s) 34, 112, 269, 355, 360, 481, 510, 968
BstACI GRCGYC 2 cut(s) 572, 596
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 669
BstEII GGTNACC 1 cut(s) 1063
BstF5I GGATG 2 cut(s) 1027, 1253
BstFNI CGCG 1 cut(s) 470
BstH2I RGCGCY 1 cut(s) 599
BstHHI GCGC 2 cut(s) 556, 598
BstKTI GATC 5 cut(s) 49, 221, 712, 742, 760
BstMBI GATC 5 cut(s) 46, 218, 709, 739, 757
BstMCI CGRYCG 1 cut(s) 1043
BstMWI GCNNNNNNNGC 5 cut(s) 23, 542, 551, 593, 1123
BstNI CCWGG 1 cut(s) 601
BstPI GGTNACC 1 cut(s) 1063
BstSCI CCNGG 2 cut(s) 599, 1067
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 2 cut(s) 950, 1113
BstV2I GAAGAC 3 cut(s) 198, 327, 495
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BstZI CGGCCG 1 cut(s) 1040
BsuRI GGCC 3 cut(s) 545, 1042, 1084
BtgZI GCGATG 1 cut(s) 711
BtsCI GGATG 2 cut(s) 1027, 1253
BtsIMutI CAGTG 1 cut(s) 871
BtuMI TCGCGA 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 556, 598
Cfr13I GGNCC 2 cut(s) 1051, 1082
Csp6I GTAC 1 cut(s) 1159
CviAII CATG 1 cut(s) 902
CviQI GTAC 1 cut(s) 1159
DdeI CTNAG 1 cut(s) 669
DinI GGCGCC 1 cut(s) 597
DpnI GATC 5 cut(s) 48, 220, 711, 741, 759
DpnII GATC 5 cut(s) 46, 218, 709, 739, 757
DraIII CACNNNGTG 1 cut(s) 817
EaeI YGGCCR 1 cut(s) 1040
EagI CGGCCG 1 cut(s) 1040
Eam1104I CTCTTC 8 cut(s) 34, 112, 269, 355, 360, 481, 510, 968
EarI CTCTTC 8 cut(s) 34, 112, 269, 355, 360, 481, 510, 968
EclXI CGGCCG 1 cut(s) 1040
Eco130I CCWWGG 1 cut(s) 1059
Eco147I AGGCCT 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 1051
Eco52I CGGCCG 1 cut(s) 1040
Eco57I CTGAAG 1 cut(s) 1175
Eco88I CYCGRG 1 cut(s) 607
Eco91I GGTNACC 1 cut(s) 1063
EcoO65I GGTNACC 1 cut(s) 1063
EcoRI GAATTC 1 cut(s) 616
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 1 cut(s) 1059
EgeI GGCGCC 1 cut(s) 597
EheI GGCGCC 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 1059
FaeI CATG 1 cut(s) 905
FaqI GGGAC 2 cut(s) 943, 977
FatI CATG 1 cut(s) 901
Fnu4HI GCNGC 6 cut(s) 524, 527, 936, 939, 1127, 1199
FokI GGATG 1 cut(s) 1034
Fsp4HI GCNGC 6 cut(s) 524, 527, 936, 939, 1127, 1199
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 555, 597
GluI GCNGC 6 cut(s) 524, 527, 936, 939, 1127, 1199
HaeII RGCGCY 1 cut(s) 599
HaeIII GGCC 3 cut(s) 545, 1042, 1084
HapII CCGG 5 cut(s) 593, 621, 633, 645, 1068
HhaI GCGC 2 cut(s) 556, 598
Hin1I GRCGYC 2 cut(s) 572, 596
Hin1II CATG 1 cut(s) 905
Hin6I GCGC 2 cut(s) 554, 596
HinP1I GCGC 2 cut(s) 554, 596
HincII GTYRAC 2 cut(s) 457, 808
HindII GTYRAC 2 cut(s) 457, 808
HinfI GANTC 5 cut(s) 5, 200, 671, 821, 1036
HpaII CCGG 5 cut(s) 593, 621, 633, 645, 1068
HphI GGTGA 3 cut(s) 851, 959, 1075
Hpy166II GTNNAC 2 cut(s) 457, 808
Hpy188I TCNGA 3 cut(s) 34, 541, 1146
Hpy188III TCNNGA 5 cut(s) 216, 469, 485, 1193, 1206
Hpy8I GTNNAC 2 cut(s) 457, 808
Hpy99I CGWCG 7 cut(s) 38, 497, 500, 503, 553, 577, 1108
HpyAV CCTTC 8 cut(s) 145, 158, 284, 345, 764, 1095, 1102, 1171
HpyCH4III ACNGT 2 cut(s) 661, 866
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 2 cut(s) 767, 1129
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 542, 551, 593, 1123
HpyF3I CTNAG 1 cut(s) 669
HpySE526I ACGT 1 cut(s) 572
Hsp92I GRCGYC 2 cut(s) 572, 596
Hsp92II CATG 1 cut(s) 905
HspAI GCGC 2 cut(s) 554, 596
KasI GGCGCC 1 cut(s) 595
Kzo9I GATC 5 cut(s) 46, 218, 709, 739, 757
LmnI GCTCC 1 cut(s) 1195
Lsp1109I GCAGC 2 cut(s) 950, 1113
LweI GCATC 1 cut(s) 1150
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 4 cut(s) 568, 791, 860, 1063
MalI GATC 5 cut(s) 48, 220, 711, 741, 759
MbiI CCGCTC 1 cut(s) 1198
MboI GATC 5 cut(s) 46, 218, 709, 739, 757
MflI RGATCY 1 cut(s) 218
MluCI AATT 5 cut(s) 142, 397, 440, 616, 751
Mly113I GGCGCC 1 cut(s) 596
MlyI GAGTC 2 cut(s) 194, 680
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 2 cut(s) 960, 1014
MspI CCGG 5 cut(s) 593, 621, 633, 645, 1068
MspR9I CCNGG 2 cut(s) 601, 1069
MvaI CCWGG 1 cut(s) 601
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 5 cut(s) 23, 542, 551, 593, 1123
NarI GGCGCC 1 cut(s) 596
NciI CCSGG 1 cut(s) 1069
NdeII GATC 5 cut(s) 46, 218, 709, 739, 757
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 905
NlaIV GGNNCC 3 cut(s) 597, 631, 643
NmeAIII GCCGAG 1 cut(s) 1068
NmuCI GTSAC 2 cut(s) 568, 1063
NruI TCGCGA 1 cut(s) 470
PceI AGGCCT 1 cut(s) 545
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 492, 498, 545
PfeI GAWTC 2 cut(s) 821, 1036
PflMI CCANNNNNTGG 1 cut(s) 817
PkrI GCNGC 6 cut(s) 525, 528, 937, 940, 1128, 1200
PleI GAGTC 2 cut(s) 194, 679
PluTI GGCGCC 1 cut(s) 599
PpsI GAGTC 2 cut(s) 194, 679
Psp6I CCWGG 1 cut(s) 599
PspEI GGTNACC 1 cut(s) 1063
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 3 cut(s) 597, 631, 643
PspPI GGNCC 2 cut(s) 1051, 1082
PsuI RGATCY 1 cut(s) 218
RruI TCGCGA 1 cut(s) 470
RsaI GTAC 1 cut(s) 1160
RsaNI GTAC 1 cut(s) 1159
SaqAI TTAA 2 cut(s) 960, 1014
SatI GCNGC 6 cut(s) 524, 527, 936, 939, 1127, 1199
Sau3AI GATC 5 cut(s) 46, 218, 709, 739, 757
Sau96I GGNCC 2 cut(s) 1051, 1082
SchI GAGTC 2 cut(s) 194, 680
ScrFI CCNGG 2 cut(s) 601, 1069
SfaNI GCATC 1 cut(s) 1150
SfoI GGCGCC 1 cut(s) 597
SinI GGWCC 1 cut(s) 1051
SmlI CTYRAG 2 cut(s) 214, 1007
SmoI CTYRAG 2 cut(s) 214, 1007
Sse9I AATT 5 cut(s) 142, 397, 440, 616, 751
SseBI AGGCCT 1 cut(s) 545
SsiI CCGC 5 cut(s) 523, 526, 837, 935, 1198
SspDI GGCGCC 1 cut(s) 595
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 599, 1067
StyI CCWWGG 1 cut(s) 1059
TaaI ACNGT 2 cut(s) 661, 866
TaiI ACGT 1 cut(s) 575
TaqI TCGA 4 cut(s) 486, 712, 848, 1103
TasI AATT 5 cut(s) 142, 397, 440, 616, 751
TatI WGTACW 1 cut(s) 1158
TauI GCSGC 4 cut(s) 526, 529, 938, 1201
TfiI GAWTC 2 cut(s) 821, 1036
Tru1I TTAA 2 cut(s) 960, 1014
Tru9I TTAA 2 cut(s) 960, 1014
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 2 cut(s) 568, 1063
TseI GCWGC 2 cut(s) 938, 1126
Tsp45I GTSAC 2 cut(s) 568, 1063
TspDTI ATGAA 4 cut(s) 321, 732, 1046, 1184
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 1 cut(s) 871
Van91I CCANNNNNTGG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 1051
XapI RAATTY 2 cut(s) 616, 751
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.