MD13G1239600.v1.1

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016126
Physical Location & Seq
Reverse (-)
24506754 .. 24510332
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1239600.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGGAATCTCTGCTTTCCAACTACGCCTCTTCCGACGAAGAAGAAGATCAACAGCAGCAGCAACCGCCACCAAAACGCACAGCTTTACCCCCAAAACCCAGCTCTCAACTTTCCTCCACTTCCTCTCTCTTTTCTTCTCTACCCAAATCCAAATCCAAACCCCAAAATCCCTCTTTCTTCCAATCCCTTCCCAAACCAAAACAAACCCTAATTCCAAAATCCCAAAATCTTGGCGATGACGATGAAGACCCAATTGATTCGATCCCCACCTCAAAACCCTCCTCGGTTTTCTCATTGCTTCCTCCGCCGAAATCCCGAAATCCAATACAACCCATCACCAATCTCTCGTCATCCGATCAAAACCCTAGAAAGATTGTCCAATTCAATCCTCCAATCAACTCATATAAGTACGATGAAGACGAAGAAGACGATGAGGAAAAAGAAACGCGGCGCCGAAAAGCGTCTGAATCCTCGGTCCAGAAGTTGTCTGCGAAGTCATTTTTGTCGTCCTTATCGGCGCCAAGGTACTCCTCCACATTGGGTGCATCCGCTGGTTTTGGTTCGGGATCGGGTTCGAGCAGGAGAGCCATTGTTGAAACGGAATCGGTTGGTTCGAGAGTGGAGAGCGGTGCGGGGGTGGAGCAGAACATAGAAAGTCATGAGAAGTTCCAATCAAATAGTGAGCAAAGTGCGGTTAACTATGAAGGGTATGGTGGTTCTGAGAATTACCAATCTACTATTGATCAAAATGCAGTTAATTATGAAAGCTATGGTGGTTACGAGGGTTACCAATCTGGTATTGATCGACATGTGGATGCTGGGGTTCAGCTACATGCAGGGGTTAGTGGTAGTGATCCTTCGAATTACGGCAATTATGATGGTTATGGGGGTTATGCTGGTTATGAGCAGCAGTATGGGAATGATTGGGTTGGTGGGTCCGGAACTGTGGTGCCAACCATCCCCGCTACTGGTGTAAGTGAGATTAAAGTTGGTAAAAAGAGGGGGAGGAATGAGGTGCCAACTGAAATAATTGAGGTTAAGCAGGACGAGTTGATGAAGAATCGGCCAAGAGAGGACCAGGCTAAGTTAACAGGAATTGCCTTTGGGCCTTCTTATCAGCCTGTTTCTACAAAAGCGAAGCCTACAAAGCTGCATAAGAGGAAGCATCAGATTGGTTCTCTGTACTTTGATATGAAGCAGAAGGAGATGGAACTCGCAGAGCGGCGTTCAAGAGGCTTCCTGACTAAAGCTGAAACACATGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

426

Amino Acids

46.63

Weight (kDa)

6.0

Isoelectric Point (pI)

58.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRCC PF10253 332 - 425 2.5e-12 Mitotic checkpoint regulator, MAD2B-interacting
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 450, 517, 949, 1015
AccBSI CCGCTC 2 cut(s) 627, 1222
AccII CGCG 1 cut(s) 448
AccIII TCCGGA 1 cut(s) 938
AciI CCGC 9 cut(s) 65, 305, 448, 549, 627, 632, 692, 963, 1222
AclWI GGATC 3 cut(s) 256, 574, 848
AcoI YGGCCR 1 cut(s) 1064
AcyI GRCGYC 2 cut(s) 451, 518
AfaI GTAC 3 cut(s) 410, 527, 1184
AfiI CCNNNNNNNGG 1 cut(s) 968
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 3 cut(s) 385, 596, 1230
AjnI CCWGG 1 cut(s) 1077
AluBI AGCT 6 cut(s) 83, 102, 768, 829, 1150, 1250
AluI AGCT 6 cut(s) 83, 102, 768, 829, 1150, 1250
AlwI GGATC 3 cut(s) 256, 574, 848
Aor13HI TCCGGA 1 cut(s) 938
AoxI GGCC 2 cut(s) 1064, 1106
ApeKI GCWGC 4 cut(s) 55, 58, 907, 1150
AspLEI GCGC 2 cut(s) 453, 520
AspS9I GGNCC 4 cut(s) 475, 936, 1075, 1106
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 860
AvaII GGWCC 3 cut(s) 475, 936, 1075
BanI GGYRCC 4 cut(s) 450, 517, 949, 1015
BarI GAAGNNNNNNTAC 2 cut(s) 841, 873
BbsI GAAGAC 3 cut(s) 252, 423, 432
BbvI GCAGC 4 cut(s) 67, 70, 919, 1137
BccI CCATC 5 cut(s) 341, 872, 965, 1201, 1266
BceAI ACGGC 1 cut(s) 883
BciT130I CCWGG 1 cut(s) 1079
BclI TGATCA 1 cut(s) 742
BfaI CTAG 1 cut(s) 366
BfoI RGCGCY 2 cut(s) 454, 521
BisI GCNGC 6 cut(s) 56, 59, 449, 908, 1151, 1223
BlsI GCNGC 6 cut(s) 57, 60, 450, 909, 1152, 1224
Bme1390I CCNGG 1 cut(s) 1079
Bme18I GGWCC 3 cut(s) 475, 936, 1075
BmgT120I GGNCC 4 cut(s) 475, 936, 1075, 1106
BmiI GGNNCC 5 cut(s) 452, 519, 937, 951, 1017
BmrFI CCNGG 1 cut(s) 1079
BmsI GCATC 3 cut(s) 554, 805, 1174
BpiI GAAGAC 3 cut(s) 252, 423, 432
Bpu14I TTCGAA 1 cut(s) 860
BsaHI GRCGYC 2 cut(s) 451, 518
BsaJI CCNNGG 3 cut(s) 282, 471, 521
BsaWI WCCGGW 1 cut(s) 938
Bsc4I CCNNNNNNNGG 1 cut(s) 968
Bse1I ACTGG 1 cut(s) 973
Bse3DI GCAATG 1 cut(s) 293
BseAI TCCGGA 1 cut(s) 938
BseBI CCWGG 1 cut(s) 1079
BseDI CCNNGG 3 cut(s) 282, 471, 521
BseGI GGATG 5 cut(s) 350, 545, 820, 957, 1277
BseLI CCNNNNNNNGG 1 cut(s) 968
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 711
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 2 cut(s) 271, 520
BseXI GCAGC 4 cut(s) 67, 70, 919, 1137
BseYI CCCAGC 2 cut(s) 98, 818
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 2 cut(s) 1066, 1108
BshNI GGYRCC 4 cut(s) 450, 517, 949, 1015
BsiSI CCGG 1 cut(s) 939
BslI CCNNNNNNNGG 1 cut(s) 968
BsnI GGCC 2 cut(s) 1066, 1108
Bsp119I TTCGAA 1 cut(s) 860
Bsp13I TCCGGA 1 cut(s) 938
Bsp143I GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
BspACI CCGC 9 cut(s) 65, 305, 448, 549, 627, 632, 692, 963, 1222
BspANI GGCC 2 cut(s) 1066, 1108
BspCNI CTCAG 1 cut(s) 712
BspEI TCCGGA 1 cut(s) 938
BspFNI CGCG 1 cut(s) 448
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 5 cut(s) 452, 519, 937, 951, 1017
BspPI GGATC 3 cut(s) 256, 574, 848
BspT104I TTCGAA 1 cut(s) 860
BspT107I GGYRCC 4 cut(s) 450, 517, 949, 1015
BsrBI CCGCTC 2 cut(s) 627, 1222
BsrDI GCAATG 1 cut(s) 293
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 3 cut(s) 282, 471, 521
BssMI GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
BssNI GRCGYC 2 cut(s) 451, 518
BssT1I CCWWGG 1 cut(s) 521
Bst2UI CCWGG 1 cut(s) 1079
Bst4CI ACNGT 1 cut(s) 946
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 2 cut(s) 451, 518
BstBI TTCGAA 1 cut(s) 860
BstDEI CTNAG 2 cut(s) 720, 1083
BstEII GGTNACC 1 cut(s) 785
BstF5I GGATG 5 cut(s) 350, 545, 820, 957, 1277
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 2 cut(s) 454, 521
BstHHI GCGC 2 cut(s) 453, 520
BstKTI GATC 7 cut(s) 49, 264, 358, 569, 745, 805, 856
BstMBI GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
BstMWI GCNNNNNNNGC 3 cut(s) 64, 304, 1147
BstNI CCWGG 1 cut(s) 1079
BstNSI RCATGY 3 cut(s) 812, 836, 1262
BstPI GGTNACC 1 cut(s) 785
BstSCI CCNGG 1 cut(s) 1077
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 4 cut(s) 67, 70, 919, 1137
BstV2I GAAGAC 3 cut(s) 252, 423, 432
BstXI CCANNNNNNTGG 1 cut(s) 230
BsuRI GGCC 2 cut(s) 1066, 1108
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 5 cut(s) 350, 545, 820, 957, 1277
CciI TCATGA 1 cut(s) 658
CfoI GCGC 2 cut(s) 453, 520
Cfr13I GGNCC 4 cut(s) 475, 936, 1075, 1106
CseI GACGC 1 cut(s) 450
Csp6I GTAC 3 cut(s) 409, 526, 1183
CviAII CATG 4 cut(s) 659, 809, 833, 1259
CviQI GTAC 3 cut(s) 409, 526, 1183
DdeI CTNAG 2 cut(s) 720, 1083
DinI GGCGCC 2 cut(s) 452, 519
DpnI GATC 7 cut(s) 48, 263, 357, 568, 744, 804, 855
DpnII GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
EaeI YGGCCR 1 cut(s) 1064
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 521
Eco47I GGWCC 3 cut(s) 475, 936, 1075
Eco91I GGTNACC 1 cut(s) 785
EcoO65I GGTNACC 1 cut(s) 785
EcoRII CCWGG 1 cut(s) 1077
EcoT14I CCWWGG 1 cut(s) 521
EgeI GGCGCC 2 cut(s) 452, 519
EheI GGCGCC 2 cut(s) 452, 519
ErhI CCWWGG 1 cut(s) 521
FaeI CATG 4 cut(s) 662, 812, 836, 1262
FatI CATG 4 cut(s) 658, 808, 832, 1258
FauI CCCGC 2 cut(s) 625, 970
FbaI TGATCA 1 cut(s) 742
Fnu4HI GCNGC 6 cut(s) 56, 59, 449, 908, 1151, 1223
FokI GGATG 4 cut(s) 337, 532, 827, 944
Fsp4HI GCNGC 6 cut(s) 56, 59, 449, 908, 1151, 1223
FspBI CTAG 1 cut(s) 366
GlaI GCGC 2 cut(s) 452, 519
GluI GCNGC 6 cut(s) 56, 59, 449, 908, 1151, 1223
GsaI CCCAGC 2 cut(s) 102, 822
HaeII RGCGCY 2 cut(s) 454, 521
HaeIII GGCC 2 cut(s) 1066, 1108
HapII CCGG 1 cut(s) 939
HgaI GACGC 1 cut(s) 450
HhaI GCGC 2 cut(s) 453, 520
Hin1I GRCGYC 2 cut(s) 451, 518
Hin1II CATG 4 cut(s) 662, 812, 836, 1262
Hin6I GCGC 2 cut(s) 451, 518
HinP1I GCGC 2 cut(s) 451, 518
HincII GTYRAC 2 cut(s) 697, 1089
HindII GTYRAC 2 cut(s) 697, 1089
HinfI GANTC 5 cut(s) 5, 257, 467, 602, 1060
HpaI GTTAAC 2 cut(s) 697, 1089
HpaII CCGG 1 cut(s) 939
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 697, 1089
Hpy188I TCNGA 5 cut(s) 34, 355, 466, 721, 1170
Hpy188III TCNNGA 8 cut(s) 315, 478, 564, 615, 659, 939, 1230, 1240
Hpy8I GTNNAC 2 cut(s) 697, 1089
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 197, 698, 867, 1119, 1195
HpyCH4III ACNGT 1 cut(s) 946
HpyCH4V TGCA 4 cut(s) 545, 752, 836, 1153
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 304, 1147
HpyF3I CTNAG 2 cut(s) 720, 1083
Hsp92I GRCGYC 2 cut(s) 451, 518
Hsp92II CATG 4 cut(s) 662, 812, 836, 1262
HspAI GCGC 2 cut(s) 451, 518
KasI GGCGCC 2 cut(s) 450, 517
Kpn2I TCCGGA 1 cut(s) 938
Ksp22I TGATCA 1 cut(s) 742
KspAI GTTAAC 2 cut(s) 697, 1089
Kzo9I GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
LmnI GCTCC 1 cut(s) 640
Lsp1109I GCAGC 4 cut(s) 67, 70, 919, 1137
LweI GCATC 3 cut(s) 554, 805, 1174
MaeI CTAG 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 776, 785
MalI GATC 7 cut(s) 48, 263, 357, 568, 744, 804, 855
MbiI CCGCTC 2 cut(s) 627, 1222
MboI GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
MfeI CAATTG 1 cut(s) 252
MluCI AATT 9 cut(s) 210, 252, 380, 724, 757, 862, 871, 1029, 1095
Mly113I GGCGCC 2 cut(s) 451, 518
MmeI TCCRAC 2 cut(s) 42, 57
MroI TCCGGA 1 cut(s) 938
MseI TTAA 5 cut(s) 696, 756, 984, 1038, 1088
MslI CAYNNNNRTG 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 1 cut(s) 939
MspR9I CCNGG 1 cut(s) 1079
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 1079
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 3 cut(s) 64, 304, 1147
NarI GGCGCC 2 cut(s) 451, 518
NdeII GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
NlaIII CATG 4 cut(s) 662, 812, 836, 1262
NlaIV GGNNCC 5 cut(s) 452, 519, 937, 951, 1017
NspI RCATGY 3 cut(s) 812, 836, 1262
NspV TTCGAA 1 cut(s) 860
PagI TCATGA 1 cut(s) 658
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 417, 426, 611
PfeI GAWTC 5 cut(s) 5, 257, 467, 602, 1060
PkrI GCNGC 6 cut(s) 57, 60, 450, 909, 1152, 1224
PluTI GGCGCC 2 cut(s) 454, 521
PscI ACATGT 1 cut(s) 808
Psp6I CCWGG 1 cut(s) 1077
PspEI GGTNACC 1 cut(s) 785
PspFI CCCAGC 2 cut(s) 98, 818
PspGI CCWGG 1 cut(s) 1077
PspN4I GGNNCC 5 cut(s) 452, 519, 937, 951, 1017
PspPI GGNCC 4 cut(s) 475, 936, 1075, 1106
RsaI GTAC 3 cut(s) 410, 527, 1184
RsaNI GTAC 3 cut(s) 409, 526, 1183
RseI CAYNNNNRTG 1 cut(s) 813
SaqAI TTAA 5 cut(s) 696, 756, 984, 1038, 1088
SatI GCNGC 6 cut(s) 56, 59, 449, 908, 1151, 1223
Sau3AI GATC 7 cut(s) 46, 261, 355, 566, 742, 802, 853
Sau96I GGNCC 4 cut(s) 475, 936, 1075, 1106
ScrFI CCNGG 1 cut(s) 1079
SfaNI GCATC 3 cut(s) 554, 805, 1174
SfoI GGCGCC 2 cut(s) 452, 519
SfuI TTCGAA 1 cut(s) 860
SinI GGWCC 3 cut(s) 475, 936, 1075
SmiMI CAYNNNNRTG 1 cut(s) 813
Sse9I AATT 9 cut(s) 210, 252, 380, 724, 757, 862, 871, 1029, 1095
SsiI CCGC 9 cut(s) 65, 305, 448, 549, 627, 632, 692, 963, 1222
SspDI GGCGCC 2 cut(s) 450, 517
SspMI CTAG 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 1077
StyI CCWWGG 1 cut(s) 521
TaaI ACNGT 1 cut(s) 946
TaqI TCGA 5 cut(s) 260, 575, 614, 805, 860
TaqII GACCGA 1 cut(s) 463
TasI AATT 9 cut(s) 210, 252, 380, 724, 757, 862, 871, 1029, 1095
TatI WGTACW 1 cut(s) 1182
TauI GCSGC 2 cut(s) 451, 1225
TfiI GAWTC 5 cut(s) 5, 257, 467, 602, 1060
Tru1I TTAA 5 cut(s) 696, 756, 984, 1038, 1088
Tru9I TTAA 5 cut(s) 696, 756, 984, 1038, 1088
TseI GCWGC 4 cut(s) 55, 58, 907, 1150
TspDTI ATGAA 6 cut(s) 258, 429, 717, 777, 1070, 1208
TspGWI ACGGA 1 cut(s) 614
VpaK11BI GGWCC 3 cut(s) 475, 936, 1075
XceI RCATGY 3 cut(s) 812, 836, 1262
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.