Rmu_sc0003135.1_g000009

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003135.1
OG0016126
Physical Location & Seq
Reverse (-)
37508 .. 40375
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003135.1_g000009.1.cds

Sequence Viewer

Length: 1269 bp
atggactctctgctagcaaactacgcctcttccgacgaagaagaagatcaaaaaccccaccaccaccaactacaaccacctccaaacgcttcttcttctcttttctcttctctacccaaacccaaacccgaaccctcatccttcttcaattcccttcctcctccaaaacaaacccaatcaaatcctccacaccaagaagaccaagactcaaaacccacctcaagatcttctcttttctcctctctacccaaacccaaaccctcctctctcttccaatcccttcccaaaccaaaacttgaaccacaacaagatgaagaagaagaagaagacataaaacccccttcttctgttttctcttctcttcctccccccaaatcccaaatccctaacaaacccatctccaattcctcatcttctcctgcccaaatccccaaaagggtcgtccaattcaagcctcccgtcaacccttatttcgcaaactcttcccatctcgaagacgacgacgacgatgaggaagaagaggagaggcggcggcgaaaagcctcggaggcctcgtcggcgcaacctttgtccgtgacgtcgttcttatcagctattccggcgcccaggaactcgggtgtgaattccggtttaggttccggtgtaggttccggtaggagagccaccgttgaaacagagtcgtttggttctaaagtggagagtgatgtgggtttggatcgaaatgaaaatgtagcaagttatgatgatccccaatcaaattttgatccaaatgcagaaggttatggtgggtatgagagttaccaatatggtgttgaccaaaatgtggattctggggttagtggcggtgatggttcgagctatggtaattacagtggttatggtgggtatactggttatgctggaaatcatgggaacgaatggggtgatgggtcggggactgcggcggcaatgggtgtgtcaggtgatactggggttaaagtgagtgggaagaggaggaggaaagatgtcccaactgaaatacttgaggttaagcaggatgagttgatgaagaatcggccgagggaggaccaagccaaggtgaccgggatagcttttgggccttcttaccagcctgtttcgacgaaggggaagccgacaaagctgcataagaggaagcatcagattggttctctgtactttgatatgaagcagaaggagatggaacttcaggagcggcgttcaagaggcttccttacaaaagctgaaacacaagccaagtatggatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

422

Amino Acids

46.03

Weight (kDa)

5.55

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 601
AccB7I CCANNNNNTGG 1 cut(s) 823
AccBSI CCGCTC 1 cut(s) 1213
AccI GTMKAC 1 cut(s) 887
AciI CCGC 6 cut(s) 529, 532, 843, 941, 944, 1213
AclWI GGATC 3 cut(s) 723, 740, 758
AcoI YGGCCR 1 cut(s) 1055
AcsI RAATTY 2 cut(s) 622, 757
AcuI CTGAAG 1 cut(s) 1190
AcyI GRCGYC 2 cut(s) 578, 602
AfaI GTAC 1 cut(s) 1175
AfiI CCNNNNNNNGG 3 cut(s) 436, 823, 1075
AgsI TTSAA 5 cut(s) 148, 299, 451, 671, 1221
AjnI CCWGG 1 cut(s) 605
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 5 cut(s) 593, 858, 1091, 1141, 1241
AluI AGCT 5 cut(s) 593, 858, 1091, 1141, 1241
AlwI GGATC 3 cut(s) 723, 740, 758
Ama87I CYCGRG 1 cut(s) 613
AoxI GGCC 3 cut(s) 549, 1055, 1097
ApeKI GCWGC 1 cut(s) 1141
ApoI RAATTY 2 cut(s) 622, 757
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AspLEI GCGC 2 cut(s) 562, 604
AspS9I GGNCC 2 cut(s) 1066, 1097
AsuC2I CCSGG 1 cut(s) 1084
AsuHPI GGTGA 4 cut(s) 857, 935, 974, 1090
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 613
AvaII GGWCC 1 cut(s) 1066
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BanI GGYRCC 1 cut(s) 601
BbsI GAAGAC 3 cut(s) 204, 333, 501
BbvI GCAGC 1 cut(s) 1128
BccI CCATC 6 cut(s) 404, 495, 842, 920, 1192, 1257
BcgI CGANNNNNNTGC 2 cut(s) 1123, 1157
BciT130I CCWGG 1 cut(s) 607
BcnI CCSGG 1 cut(s) 1084
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 605
BglI GCCNNNNNGGC 2 cut(s) 548, 557
BglII AGATCT 1 cut(s) 224
BisI GCNGC 6 cut(s) 530, 533, 942, 945, 1142, 1214
BlsI GCNGC 6 cut(s) 531, 534, 943, 946, 1143, 1215
Bme1390I CCNGG 2 cut(s) 607, 1084
Bme18I GGWCC 1 cut(s) 1066
BmeT110I CYCGRG 1 cut(s) 613
BmgT120I GGNCC 2 cut(s) 1066, 1097
BmiI GGNNCC 3 cut(s) 603, 637, 649
BmrFI CCNGG 2 cut(s) 607, 1084
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 1 cut(s) 1165
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 978
BpiI GAAGAC 3 cut(s) 204, 333, 501
BpuEI CTTGAG 2 cut(s) 205, 1043
BpuMI CCSGG 1 cut(s) 1084
BsaHI GRCGYC 2 cut(s) 578, 602
BsaJI CCNNGG 4 cut(s) 543, 605, 1058, 1074
BsaWI WCCGGW 3 cut(s) 626, 638, 650
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 3 cut(s) 436, 823, 1075
Bse1I ACTGG 2 cut(s) 895, 973
Bse3DI GCAATG 1 cut(s) 954
BseBI CCWGG 1 cut(s) 607
BseDI CCNNGG 4 cut(s) 543, 605, 1058, 1074
BseGI GGATG 3 cut(s) 137, 1042, 1268
BseLI CCNNNNNNNGG 3 cut(s) 436, 823, 1075
BseMI GCAATG 1 cut(s) 954
BseNI ACTGG 2 cut(s) 895, 973
BseRI GAGGAG 6 cut(s) 150, 229, 253, 536, 1006, 1009
BseX3I CGGCCG 1 cut(s) 1055
BseXI GCAGC 1 cut(s) 1128
Bsh1285I CGRYCG 1 cut(s) 1058
BshFI GGCC 3 cut(s) 551, 1057, 1099
BshNI GGYRCC 1 cut(s) 601
BsiEI CGRYCG 1 cut(s) 1058
BsiHKCI CYCGRG 1 cut(s) 613
BsiSI CCGG 5 cut(s) 599, 627, 639, 651, 1083
BslFI GGGAC 2 cut(s) 949, 992
BslI CCNNNNNNNGG 3 cut(s) 436, 823, 1075
BsmFI GGGAC 2 cut(s) 949, 992
BsnI GGCC 3 cut(s) 551, 1057, 1099
BsoBI CYCGRG 1 cut(s) 613
Bsp143I GATC 5 cut(s) 46, 224, 715, 745, 763
BspACI CCGC 6 cut(s) 529, 532, 843, 941, 944, 1213
BspANI GGCC 3 cut(s) 551, 1057, 1099
BspLI GGNNCC 3 cut(s) 603, 637, 649
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 723, 740, 758
BspT107I GGYRCC 1 cut(s) 601
BsrBI CCGCTC 1 cut(s) 1213
BsrDI GCAATG 1 cut(s) 954
BsrI ACTGG 2 cut(s) 895, 973
BssECI CCNNGG 4 cut(s) 543, 605, 1058, 1074
BssMI GATC 5 cut(s) 46, 224, 715, 745, 763
BssNAI GTATAC 1 cut(s) 888
BssNI GRCGYC 2 cut(s) 578, 602
BssT1I CCWWGG 1 cut(s) 1074
Bst1107I GTATAC 1 cut(s) 888
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 667, 872
Bst6I CTCTTC 8 cut(s) 34, 112, 275, 361, 366, 487, 513, 983
BstACI GRCGYC 2 cut(s) 578, 602
BstC8I GCNNGC 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 1078
BstF5I GGATG 3 cut(s) 137, 1042, 1268
BstH2I RGCGCY 1 cut(s) 605
BstHHI GCGC 2 cut(s) 562, 604
BstKTI GATC 5 cut(s) 49, 227, 718, 748, 766
BstMBI GATC 5 cut(s) 46, 224, 715, 745, 763
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 5 cut(s) 23, 548, 557, 599, 1138
BstNI CCWGG 1 cut(s) 607
BstPI GGTNACC 1 cut(s) 1078
BstSCI CCNGG 2 cut(s) 605, 1082
BstV1I GCAGC 1 cut(s) 1128
BstV2I GAAGAC 3 cut(s) 204, 333, 501
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 888
BstZI CGGCCG 1 cut(s) 1055
BsuRI GGCC 3 cut(s) 551, 1057, 1099
BtsCI GGATG 3 cut(s) 137, 1042, 1268
BtsIMutI CAGTG 1 cut(s) 877
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 562, 604
Cfr13I GGNCC 2 cut(s) 1066, 1097
Csp6I GTAC 1 cut(s) 1174
CviAII CATG 1 cut(s) 908
CviQI GTAC 1 cut(s) 1174
DinI GGCGCC 1 cut(s) 603
DpnI GATC 5 cut(s) 48, 226, 717, 747, 765
DpnII GATC 5 cut(s) 46, 224, 715, 745, 763
EaeI YGGCCR 1 cut(s) 1055
EagI CGGCCG 1 cut(s) 1055
Eam1104I CTCTTC 8 cut(s) 34, 112, 275, 361, 366, 487, 513, 983
EarI CTCTTC 8 cut(s) 34, 112, 275, 361, 366, 487, 513, 983
EclXI CGGCCG 1 cut(s) 1055
Eco130I CCWWGG 1 cut(s) 1074
Eco147I AGGCCT 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 1066
Eco52I CGGCCG 1 cut(s) 1055
Eco57I CTGAAG 1 cut(s) 1190
Eco88I CYCGRG 1 cut(s) 613
Eco91I GGTNACC 1 cut(s) 1078
EcoO65I GGTNACC 1 cut(s) 1078
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 605
EcoT14I CCWWGG 1 cut(s) 1074
EgeI GGCGCC 1 cut(s) 603
EheI GGCGCC 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 1074
FaeI CATG 1 cut(s) 911
FaqI GGGAC 2 cut(s) 949, 992
FatI CATG 1 cut(s) 907
FblI GTMKAC 1 cut(s) 887
Fnu4HI GCNGC 6 cut(s) 530, 533, 942, 945, 1142, 1214
FokI GGATG 2 cut(s) 124, 1049
Fsp4HI GCNGC 6 cut(s) 530, 533, 942, 945, 1142, 1214
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 561, 603
GluI GCNGC 6 cut(s) 530, 533, 942, 945, 1142, 1214
HaeII RGCGCY 1 cut(s) 605
HaeIII GGCC 3 cut(s) 551, 1057, 1099
HapII CCGG 5 cut(s) 599, 627, 639, 651, 1083
HhaI GCGC 2 cut(s) 562, 604
Hin1I GRCGYC 2 cut(s) 578, 602
Hin1II CATG 1 cut(s) 911
Hin6I GCGC 2 cut(s) 560, 602
HinP1I GCGC 2 cut(s) 560, 602
HincII GTYRAC 2 cut(s) 463, 814
HindII GTYRAC 2 cut(s) 463, 814
HinfI GANTC 5 cut(s) 5, 206, 677, 827, 1051
HpaII CCGG 5 cut(s) 599, 627, 639, 651, 1083
HphI GGTGA 4 cut(s) 857, 935, 974, 1090
Hpy166II GTNNAC 3 cut(s) 463, 814, 888
Hpy188I TCNGA 3 cut(s) 34, 547, 1161
Hpy188III TCNNGA 4 cut(s) 222, 491, 1208, 1221
Hpy8I GTNNAC 3 cut(s) 463, 814, 888
Hpy99I CGWCG 7 cut(s) 38, 503, 506, 509, 559, 583, 1123
HpyAV CCTTC 8 cut(s) 151, 164, 290, 351, 770, 1110, 1117, 1186
HpyCH4III ACNGT 2 cut(s) 667, 872
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 2 cut(s) 773, 1144
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 548, 557, 599, 1138
HpySE526I ACGT 1 cut(s) 578
Hsp92I GRCGYC 2 cut(s) 578, 602
Hsp92II CATG 1 cut(s) 911
HspAI GCGC 2 cut(s) 560, 602
KasI GGCGCC 1 cut(s) 601
Kzo9I GATC 5 cut(s) 46, 224, 715, 745, 763
LmnI GCTCC 1 cut(s) 1210
Lsp1109I GCAGC 1 cut(s) 1128
LweI GCATC 1 cut(s) 1165
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 3 cut(s) 574, 797, 1078
MalI GATC 5 cut(s) 48, 226, 717, 747, 765
MbiI CCGCTC 1 cut(s) 1213
MboI GATC 5 cut(s) 46, 224, 715, 745, 763
MflI RGATCY 1 cut(s) 224
MluCI AATT 6 cut(s) 148, 403, 446, 622, 757, 865
Mly113I GGCGCC 1 cut(s) 602
MlyI GAGTC 2 cut(s) 200, 686
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 2 cut(s) 975, 1029
MspI CCGG 5 cut(s) 599, 627, 639, 651, 1083
MspR9I CCNGG 2 cut(s) 607, 1084
MvaI CCWGG 1 cut(s) 607
MwoI GCNNNNNNNGC 5 cut(s) 23, 548, 557, 599, 1138
NarI GGCGCC 1 cut(s) 602
NciI CCSGG 1 cut(s) 1084
NdeII GATC 5 cut(s) 46, 224, 715, 745, 763
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 911
NlaIV GGNNCC 3 cut(s) 603, 637, 649
NmeAIII GCCGAG 1 cut(s) 1083
NmuCI GTSAC 2 cut(s) 574, 1078
PceI AGGCCT 1 cut(s) 551
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 498, 504, 551
PfeI GAWTC 2 cut(s) 827, 1051
PflMI CCANNNNNTGG 1 cut(s) 823
PkrI GCNGC 6 cut(s) 531, 534, 943, 946, 1143, 1215
PleI GAGTC 2 cut(s) 200, 685
PluTI GGCGCC 1 cut(s) 605
PpsI GAGTC 2 cut(s) 200, 685
Psp6I CCWGG 1 cut(s) 605
PspEI GGTNACC 1 cut(s) 1078
PspGI CCWGG 1 cut(s) 605
PspN4I GGNNCC 3 cut(s) 603, 637, 649
PspPI GGNCC 2 cut(s) 1066, 1097
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 1 cut(s) 1175
RsaNI GTAC 1 cut(s) 1174
SaqAI TTAA 2 cut(s) 975, 1029
SatI GCNGC 6 cut(s) 530, 533, 942, 945, 1142, 1214
Sau3AI GATC 5 cut(s) 46, 224, 715, 745, 763
Sau96I GGNCC 2 cut(s) 1066, 1097
SchI GAGTC 2 cut(s) 200, 686
ScrFI CCNGG 2 cut(s) 607, 1084
SfaNI GCATC 1 cut(s) 1165
SfoI GGCGCC 1 cut(s) 603
SinI GGWCC 1 cut(s) 1066
SmlI CTYRAG 2 cut(s) 220, 1022
SmoI CTYRAG 2 cut(s) 220, 1022
Sse9I AATT 6 cut(s) 148, 403, 446, 622, 757, 865
SseBI AGGCCT 1 cut(s) 551
SsiI CCGC 6 cut(s) 529, 532, 843, 941, 944, 1213
SspDI GGCGCC 1 cut(s) 601
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 605, 1082
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 2 cut(s) 667, 872
TaiI ACGT 1 cut(s) 581
TaqI TCGA 4 cut(s) 492, 718, 854, 1118
TasI AATT 6 cut(s) 148, 403, 446, 622, 757, 865
TatI WGTACW 1 cut(s) 1173
TauI GCSGC 5 cut(s) 532, 535, 944, 947, 1216
TfiI GAWTC 2 cut(s) 827, 1051
Tru1I TTAA 2 cut(s) 975, 1029
Tru9I TTAA 2 cut(s) 975, 1029
TscAI CASTG 1 cut(s) 877
TseFI GTSAC 2 cut(s) 574, 1078
TseI GCWGC 1 cut(s) 1141
Tsp45I GTSAC 2 cut(s) 574, 1078
TspDTI ATGAA 4 cut(s) 327, 738, 1061, 1199
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 1 cut(s) 877
Van91I CCANNNNNTGG 1 cut(s) 823
VpaK11BI GGWCC 1 cut(s) 1066
XapI RAATTY 2 cut(s) 622, 757
XmiI GTMKAC 1 cut(s) 887
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.