AT2G20470

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001303
Physical Location & Seq
Reverse (-)
8826017 .. 8829771
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20470.1

Sequence Viewer

Length: 1710 bp
ATGGATTCTGCAAAGGGTTGGTTTCAGAAGCGGCAGATGCGTGGAGGATCCAGATATAAAGGTGCTTCAGGTGGTGCTGGTGGAGGTGGAAGTAATGGCTCTGCCGATGAGCACAACGTCGAAACTGATGAAGAAGCTGTTTCAAACACTACCAAACAGAAAGTTGCAGCAGCTAAACAGTACATTGAGAATCATTACAAAGAGCAGATGAAGATTCTACAAGAGAGGAAAGAAAGGCGAAGCATGTTAGAGCAGAAACTGGCAGATGCTGACGTGTCTGAAGAAGATCAAAATAATCTTCTCAAGTTTTTAGAGAAGAAGGAGACAGAGTACATGCGCCTTCAACGCCATAAGTTAGGTGTTGCTGATTTTGATTTGCTTACAATGATTGGGAAAGGTGCATTTGGGGAGGTTAGAGTTTGTAGAGAAAAGACGACAGGTCAAGTTTATGCAATGAAAAAGCTCAAGAAGGCTGAGATGCTTCGCAGAGGCCAGGTTGAACATGTGAGAGCAGAGAGGAACTTACTTGCGGAAGTGGACAGTAACTACATAGTGAAGCTCTACTGTTCATTTCAAGACGATGACCATCTTTACCTTGTGATGGAGTATTTGCCTGGTGGAGATATGATGACTCTTCTTATGCGCAAAGATACTTTGACAGAAGAGGAGGCCAAGTTCTATGTCGCTGAGACAGTTCTCGCTATCGAGTCCATCCACAGGCACAATTACATCCACAGGGATATCAAGCCAGACAACTTGCTGCTTGACAGATATGGGCATCTAAGACTTTCAGATTTTGGATTGTGTAAACCTTTGGACTGCAGTGCCATTGGAGAAAACGATTTTTCAAACAATTCCAATGGATCTACGGAGCAAGAGGCTGGATCAACTGCTCCAAAACGCACACAGCAGGAACAACTTGAACATTGGCAGAGGAACAGGAGAACGCTAGCTTATTCCACAGTTGGCACACCAGATTACATAGCTCCAGAAGTTCTGTTGAAGAAAGGCTATGGGATGGAGTGTGACTGGTGGTCTCTTGGTGCTATAATGTACGAAATGCTAGTAGGATATCCGCCGTTTTATTCAGATGATCCTATGTCAACCTGTAGAAAGATAGTAAACTGGAAAAGTCATTTGAAGTTTCCAGAGGAAGCAATATTGTCAAGGGAAGCAAAGGATCTTATTAACAGTCTGTTGTGCAGTGTCAGACGTAGGCTTGGTTCCAAAGGTGCTGATGAATTAAAGGCTCATACATGGTTTGAAACTGTTGATTGGGATACAATATTCGATATGGATGCAGCTTTTGTTCCAGAGGTCAATGACGATTTAGACACTCAGAATTTTGAGAAGTTTGACGAGTCTGAATCGGAAACTCAGACATCATCCAAGTCTGGCCCGTGGAGGAAGATGTTGTCGTCAAAAGACATAAACTTCGTAGGGTATACATACAAGAACTTTGAGATTGTTAATGATTACCAAGTTCCTGGAATGGCGGAGTTGAAGAAGAAGAAAAAGTCGACGCGACCAATGGTCAAGTCACTCTTCGATAATGGATCATCGGAGACCTCAGATTCATCGGAAACAACGTCGAGACCGCCTTGTGATCGACCTCCTCCTGCTCCTCCGGTTGTTCAGGGAAGCTTTCTGAAACTTCTACCACCAGAACTTGAAGTGAGACCAAAGCAAGAAGGATCTGAAGCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

569

Amino Acids

64.84

Weight (kDa)

5.73

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 126 - 273 1.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 126 - 272 2.2e-22 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 198 - 273 8.1e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 318 - 421 2e-20 Protein kinase domain
Pkinase_C PF00433 440 - 484 4.3e-08 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 644
AccI GTMKAC 2 cut(s) 1445, 1520
AccII CGCG 1 cut(s) 1525
AciI CCGC 5 cut(s) 31, 530, 1076, 1496, 1598
AclWI GGATC 8 cut(s) 42, 55, 871, 892, 1088, 1188, 1564, 1702
AcsI RAATTY 1 cut(s) 1342
AcuI CTGAAG 2 cut(s) 51, 300
AfaI GTAC 3 cut(s) 182, 332, 1055
AfiI CCNNNNNNNGG 2 cut(s) 601, 717
AflIII ACRYGT 2 cut(s) 273, 502
AhdI GACNNNNNGTC 3 cut(s) 438, 1033, 1532
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 3 cut(s) 492, 613, 1486
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 9 cut(s) 137, 173, 463, 559, 953, 986, 1304, 1644, 1703
AluI AGCT 9 cut(s) 137, 173, 463, 559, 953, 986, 1304, 1644, 1703
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 6 cut(s) 317, 683, 1041, 1559, 1588, 1672
AlwI GGATC 8 cut(s) 42, 55, 871, 892, 1088, 1188, 1564, 1702
AlwNI CAGNNNCTG 2 cut(s) 259, 269
AoxI GGCC 3 cut(s) 490, 669, 1396
ApeKI GCWGC 4 cut(s) 167, 170, 760, 1301
ApoI RAATTY 1 cut(s) 1342
ArsI GACNNNNNNTTYG 2 cut(s) 1418, 1450
AspLEI GCGC 2 cut(s) 339, 645
AspS9I GGNCC 1 cut(s) 1397
AsuNHI GCTAGC 1 cut(s) 949
BamHI GGATCC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 4 cut(s) 179, 182, 747, 1313
BccI CCATC 4 cut(s) 594, 595, 719, 1012
BceAI ACGGC 1 cut(s) 1063
BcgI CGANNNNNNTGC 2 cut(s) 1280, 1314
BciT130I CCWGG 3 cut(s) 494, 615, 1488
BciVI GTATCC 1 cut(s) 1273
BcoDI GTCTC 6 cut(s) 317, 683, 1041, 1559, 1588, 1672
BfaI CTAG 2 cut(s) 950, 1064
BfmI CTRYAG 2 cut(s) 820, 1108
BfuI GTATCC 1 cut(s) 1273
BisI GCNGC 5 cut(s) 32, 168, 171, 761, 1302
BlsI GCNGC 5 cut(s) 33, 169, 172, 762, 1303
Bme1390I CCNGG 3 cut(s) 494, 615, 1488
BmeRI GACNNNNNGTC 3 cut(s) 438, 1033, 1532
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 1397
BmiI GGNNCC 2 cut(s) 49, 1225
BmrFI CCNGG 3 cut(s) 494, 615, 1488
BmsI GCATC 5 cut(s) 27, 256, 468, 787, 1288
BmtI GCTAGC 1 cut(s) 953
BpmI CTGGAG 1 cut(s) 972
BpuEI CTTGAG 2 cut(s) 287, 449
BsaBI GATNNNNATC 1 cut(s) 585
BsaI GGTCTC 4 cut(s) 1041, 1559, 1588, 1672
BsaJI CCNNGG 1 cut(s) 1400
BsaWI WCCGGW 1 cut(s) 1627
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 659, 689
Bsc4I CCNNNNNNNGG 2 cut(s) 601, 717
Bse1I ACTGG 3 cut(s) 264, 1034, 1130
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 3 cut(s) 494, 615, 1488
BseDI CCNNGG 1 cut(s) 1400
BseGI GGATG 5 cut(s) 711, 729, 1023, 1303, 1385
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 601, 717
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 5 cut(s) 465, 678, 1352, 1391, 1584
BseNI ACTGG 3 cut(s) 264, 1034, 1130
BseRI GAGGAG 3 cut(s) 680, 1605, 1614
BseXI GCAGC 4 cut(s) 179, 182, 747, 1313
BsgI GTGCAG 1 cut(s) 1222
Bsh1236I CGCG 1 cut(s) 1525
BshFI GGCC 3 cut(s) 492, 671, 1398
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 1628
BslI CCNNNNNNNGG 2 cut(s) 601, 717
BsmAI GTCTC 6 cut(s) 317, 683, 1041, 1559, 1588, 1672
BsnI GGCC 3 cut(s) 492, 671, 1398
Bso31I GGTCTC 4 cut(s) 1041, 1559, 1588, 1672
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
BspACI CCGC 5 cut(s) 31, 530, 1076, 1496, 1598
BspANI GGCC 3 cut(s) 492, 671, 1398
BspCNI CTCAG 5 cut(s) 466, 679, 1351, 1390, 1583
BspFNI CGCG 1 cut(s) 1525
BspLI GGNNCC 2 cut(s) 49, 1225
BspMAI CTGCAG 1 cut(s) 824
BspOI GCTAGC 1 cut(s) 953
BspPI GGATC 8 cut(s) 42, 55, 871, 892, 1088, 1188, 1564, 1702
BspTNI GGTCTC 4 cut(s) 1041, 1559, 1588, 1672
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 3 cut(s) 264, 1034, 1130
BssECI CCNNGG 1 cut(s) 1400
BssMI GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
BssNAI GTATAC 1 cut(s) 1446
Bst1107I GTATAC 1 cut(s) 1446
Bst2UI CCWGG 3 cut(s) 494, 615, 1488
Bst4CI ACNGT 7 cut(s) 180, 542, 566, 694, 964, 1193, 1270
Bst6I CTCTTC 3 cut(s) 639, 657, 1550
BstC8I GCNNGC 2 cut(s) 951, 1705
BstDEI CTNAG 6 cut(s) 474, 687, 782, 1338, 1377, 1570
BstDSI CCRYGG 1 cut(s) 1400
BstF5I GGATG 5 cut(s) 711, 729, 1023, 1303, 1385
BstFNI CGCG 1 cut(s) 1525
BstHHI GCGC 2 cut(s) 339, 645
BstKTI GATC 9 cut(s) 50, 289, 866, 887, 1096, 1183, 1559, 1609, 1697
BstMAI GTCTC 6 cut(s) 317, 683, 1041, 1559, 1588, 1672
BstMBI GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
BstMWI GCNNNNNNNGC 2 cut(s) 37, 345
BstNI CCWGG 3 cut(s) 494, 615, 1488
BstNSI RCATGY 3 cut(s) 247, 337, 506
BstSCI CCNGG 3 cut(s) 492, 613, 1486
BstSFI CTRYAG 2 cut(s) 820, 1108
BstUI CGCG 1 cut(s) 1525
BstV1I GCAGC 4 cut(s) 179, 182, 747, 1313
BstX2I RGATCY 4 cut(s) 47, 863, 1180, 1694
BstXI CCANNNNNNTGG 1 cut(s) 1487
BstYI RGATCY 4 cut(s) 47, 863, 1180, 1694
BstZ17I GTATAC 1 cut(s) 1446
BsuI GTATCC 1 cut(s) 1273
BsuRI GGCC 3 cut(s) 492, 671, 1398
BtgI CCRYGG 1 cut(s) 1400
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 5 cut(s) 711, 729, 1023, 1303, 1385
BtsI GCAGTG 2 cut(s) 829, 1210
BtsIMutI CAGTG 2 cut(s) 829, 1210
Cac8I GCNNGC 2 cut(s) 951, 1705
CaiI CAGNNNCTG 2 cut(s) 259, 269
CfoI GCGC 2 cut(s) 339, 645
Cfr13I GGNCC 1 cut(s) 1397
CseI GACGC 1 cut(s) 1531
Csp6I GTAC 3 cut(s) 181, 331, 1054
CviAII CATG 4 cut(s) 244, 334, 503, 1257
CviQI GTAC 3 cut(s) 181, 331, 1054
DdeI CTNAG 6 cut(s) 474, 687, 782, 1338, 1377, 1570
DpnI GATC 9 cut(s) 49, 288, 865, 886, 1095, 1182, 1558, 1608, 1696
DpnII GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
DriI GACNNNNNGTC 3 cut(s) 438, 1033, 1532
Eam1104I CTCTTC 3 cut(s) 639, 657, 1550
Eam1105I GACNNNNNGTC 3 cut(s) 438, 1033, 1532
EarI CTCTTC 3 cut(s) 639, 657, 1550
EciI GGCGGA 2 cut(s) 1065, 1511
Eco31I GGTCTC 4 cut(s) 1041, 1559, 1588, 1672
Eco32I GATATC 2 cut(s) 742, 1073
Eco57I CTGAAG 2 cut(s) 51, 300
EcoRII CCWGG 3 cut(s) 492, 613, 1486
EcoRV GATATC 2 cut(s) 742, 1073
FaeI CATG 4 cut(s) 247, 337, 506, 1260
FalI AAGNNNNNCTT 2 cut(s) 1529, 1561
FatI CATG 4 cut(s) 243, 333, 502, 1256
FblI GTMKAC 2 cut(s) 1445, 1520
Fnu4HI GCNGC 5 cut(s) 32, 168, 171, 761, 1302
FokI GGATG 5 cut(s) 698, 716, 1030, 1310, 1372
Fsp4HI GCNGC 5 cut(s) 32, 168, 171, 761, 1302
FspBI CTAG 2 cut(s) 950, 1064
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 2 cut(s) 338, 644
GluI GCNGC 5 cut(s) 32, 168, 171, 761, 1302
GsuI CTGGAG 1 cut(s) 972
HaeIII GGCC 3 cut(s) 492, 671, 1398
HapII CCGG 1 cut(s) 1628
HgaI GACGC 1 cut(s) 1531
HhaI GCGC 2 cut(s) 339, 645
Hin1II CATG 4 cut(s) 247, 337, 506, 1260
Hin6I GCGC 2 cut(s) 337, 643
HinP1I GCGC 2 cut(s) 337, 643
HincII GTYRAC 2 cut(s) 1104, 1521
HindII GTYRAC 2 cut(s) 1104, 1521
HindIII AAGCTT 2 cut(s) 1642, 1701
HinfI GANTC 8 cut(s) 5, 190, 214, 631, 707, 1361, 1367, 1574
HpaII CCGG 1 cut(s) 1628
Hpy166II GTNNAC 6 cut(s) 538, 809, 1104, 1123, 1446, 1521
Hpy188III TCNNGA 7 cut(s) 51, 466, 575, 989, 1148, 1313, 1593
Hpy8I GTNNAC 6 cut(s) 538, 809, 1104, 1123, 1446, 1521
Hpy99I CGWCG 3 cut(s) 122, 1525, 1594
HpyAV CCTTC 4 cut(s) 313, 350, 463, 1685
HpyCH4III ACNGT 7 cut(s) 180, 542, 566, 694, 964, 1193, 1270
HpyCH4IV ACGT 4 cut(s) 117, 273, 1213, 1589
HpyCH4V TGCA 7 cut(s) 11, 167, 401, 452, 822, 1203, 1301
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 345
HpyF3I CTNAG 6 cut(s) 474, 687, 782, 1338, 1377, 1570
HpySE526I ACGT 4 cut(s) 117, 273, 1213, 1589
Hsp92II CATG 4 cut(s) 247, 337, 506, 1260
HspAI GCGC 2 cut(s) 337, 643
Kzo9I GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
LmnI GCTCC 4 cut(s) 871, 898, 991, 1627
Lsp1109I GCAGC 4 cut(s) 179, 182, 747, 1313
LweI GCATC 5 cut(s) 27, 256, 468, 787, 1288
MaeI CTAG 2 cut(s) 950, 1064
MaeII ACGT 4 cut(s) 117, 273, 1213, 1589
MaeIII GTNAC 3 cut(s) 542, 1025, 1539
MalI GATC 9 cut(s) 49, 288, 865, 886, 1095, 1182, 1558, 1608, 1696
MboI GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
MflI RGATCY 4 cut(s) 47, 863, 1180, 1694
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 4 cut(s) 724, 853, 1241, 1342
MlyI GAGTC 3 cut(s) 625, 716, 1370
MseI TTAA 3 cut(s) 1188, 1244, 1470
MspI CCGG 1 cut(s) 1628
MspR9I CCNGG 3 cut(s) 494, 615, 1488
MvaI CCWGG 3 cut(s) 494, 615, 1488
MvnI CGCG 1 cut(s) 1525
MwoI GCNNNNNNNGC 2 cut(s) 37, 345
NdeII GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
NheI GCTAGC 1 cut(s) 949
NlaIII CATG 4 cut(s) 247, 337, 506, 1260
NlaIV GGNNCC 2 cut(s) 49, 1225
NmuCI GTSAC 2 cut(s) 1025, 1539
NsbI TGCGCA 1 cut(s) 644
NspI RCATGY 3 cut(s) 247, 337, 506
PciI ACATGT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 1586
PfeI GAWTC 5 cut(s) 5, 190, 214, 1367, 1574
PfoI TCCNGGA 1 cut(s) 1486
PkrI GCNGC 5 cut(s) 33, 169, 172, 762, 1303
PleI GAGTC 3 cut(s) 625, 715, 1369
PpsI GAGTC 3 cut(s) 625, 715, 1369
PscI ACATGT 1 cut(s) 502
Psp6I CCWGG 3 cut(s) 492, 613, 1486
PspGI CCWGG 3 cut(s) 492, 613, 1486
PspN4I GGNNCC 2 cut(s) 49, 1225
PspPI GGNCC 1 cut(s) 1397
PsrI GAACNNNNNNTAC 2 cut(s) 1207, 1239
PstI CTGCAG 1 cut(s) 824
PstNI CAGNNNCTG 2 cut(s) 259, 269
PsuI RGATCY 4 cut(s) 47, 863, 1180, 1694
RsaI GTAC 3 cut(s) 182, 332, 1055
RsaNI GTAC 3 cut(s) 181, 331, 1054
SalI GTCGAC 1 cut(s) 1519
SaqAI TTAA 3 cut(s) 1188, 1244, 1470
SatI GCNGC 5 cut(s) 32, 168, 171, 761, 1302
Sau3AI GATC 9 cut(s) 47, 286, 863, 884, 1093, 1180, 1556, 1606, 1694
Sau96I GGNCC 1 cut(s) 1397
SchI GAGTC 3 cut(s) 625, 716, 1370
ScrFI CCNGG 3 cut(s) 494, 615, 1488
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 5 cut(s) 27, 256, 468, 787, 1288
SfcI CTRYAG 2 cut(s) 820, 1108
SmlI CTYRAG 2 cut(s) 302, 464
SmoI CTYRAG 2 cut(s) 302, 464
Sse9I AATT 4 cut(s) 724, 853, 1241, 1342
SsiI CCGC 5 cut(s) 31, 530, 1076, 1496, 1598
SspI AATATT 2 cut(s) 1161, 1287
SspMI CTAG 2 cut(s) 950, 1064
StyD4I CCNGG 3 cut(s) 492, 613, 1486
TaaI ACNGT 7 cut(s) 180, 542, 566, 694, 964, 1193, 1270
TaiI ACGT 4 cut(s) 120, 276, 1216, 1592
TaqI TCGA 7 cut(s) 120, 705, 1290, 1520, 1548, 1592, 1609
TasI AATT 4 cut(s) 724, 853, 1241, 1342
TatI WGTACW 2 cut(s) 180, 330
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 5 cut(s) 5, 190, 214, 1367, 1574
Tru1I TTAA 3 cut(s) 1188, 1244, 1470
Tru9I TTAA 3 cut(s) 1188, 1244, 1470
TscAI CASTG 2 cut(s) 829, 1210
TseFI GTSAC 2 cut(s) 1025, 1539
TseI GCWGC 4 cut(s) 167, 170, 760, 1301
Tsp45I GTSAC 2 cut(s) 1025, 1539
TspDTI ATGAA 6 cut(s) 144, 224, 470, 558, 1254, 1566
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 2 cut(s) 829, 1210
XapI RAATTY 1 cut(s) 1342
XceI RCATGY 3 cut(s) 247, 337, 506
XmiI GTMKAC 2 cut(s) 1445, 1520
XspI CTAG 2 cut(s) 950, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.