Rorug06G0190600

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001303
Physical Location & Seq
Forward (+)
30546898 .. 30549322
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0190600.1

Sequence Viewer

Length: 549 bp
ATGAGCCGACATCCTATTGTCAAGTGGGCTCAGCGGCCTGATACGCTTTACATTACTATTGACTTGCCTGATGCCCAGGATGTAAAGCTCAAACTGGAGCCTGAAGGAAAGTTTCTATTCTCTGCTACCACTGGACCAGAAAAGACACCCTATGAAGTTGATCTTGATCTCTATGACAAAATTGATGTAAATGAGAGTAAGGCTAGTATTGGCTTGAGAAATATCCGTTACCTAGTGACAAAGGCTGAAAATAAATGGTGGAGCAGATTGATAAAACAAGAAGGAAAACCTCCTGTGTTCTTGAAAGTTGATTGGGATAAATGGGTAGATGAGGATGAGGAGGAAGGCCAAGGACTTGGAAATGACATGGACTTTGGCGACATGGATTTCTCTAAGCTGAATATGGGTGGTGGGGGCGATGGTCTTGATGAATTCGACGATAGCAGTGACACTGAAGACGAGACCGCTGAAGCAGCACCATCTGCCTCTGCTGAATCTGATACCAAGCCTACTGCAAGTATGGAGCATGAAGCAAAACCTTCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

182

Amino Acids

20.38

Weight (kDa)

4.34

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 7 - 81 1.1e-10 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 34, 465
AcsI RAATTY 1 cut(s) 431
AcuI CTGAAG 4 cut(s) 123, 474, 489, 525
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 2 cut(s) 88, 397
AluI AGCT 2 cut(s) 88, 397
Alw26I GTCTC 1 cut(s) 455
AoxI GGCC 2 cut(s) 35, 346
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 1 cut(s) 431
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspS9I GGNCC 1 cut(s) 134
AvaII GGWCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 485
BccI CCATC 2 cut(s) 413, 487
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 2 cut(s) 204, 233
BisI GCNGC 2 cut(s) 35, 474
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 2 cut(s) 36, 475
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 116
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 235
BsaBI GATNNNNATC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 75, 349
Bse1I ACTGG 2 cut(s) 99, 136
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 75, 349
BseGI GGATG 3 cut(s) 10, 85, 340
BseJI GATNNNNATC 1 cut(s) 165
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 2 cut(s) 99, 136
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 485
BshFI GGCC 2 cut(s) 37, 348
BsmAI GTCTC 1 cut(s) 455
BsnI GGCC 2 cut(s) 37, 348
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 2 cut(s) 160, 166
Bsp1720I GCTNAGC 1 cut(s) 30
BspACI CCGC 2 cut(s) 34, 465
BspANI GGCC 2 cut(s) 37, 348
BspCNI CTCAG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 455
BsrI ACTGG 2 cut(s) 99, 136
BssECI CCNNGG 2 cut(s) 75, 349
BssMI GATC 2 cut(s) 160, 166
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 77
BstAPI GCANNNNNTGC 1 cut(s) 482
BstDEI CTNAG 2 cut(s) 30, 393
BstF5I GGATG 3 cut(s) 10, 85, 340
BstKTI GATC 2 cut(s) 163, 169
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 2 cut(s) 160, 166
BstMWI GCNNNNNNNGC 3 cut(s) 43, 473, 482
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 356
BsuRI GGCC 2 cut(s) 37, 348
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 3 cut(s) 10, 85, 340
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 3 cut(s) 129, 450, 451
Cfr13I GGNCC 1 cut(s) 134
CviAII CATG 3 cut(s) 367, 382, 527
DdeI CTNAG 2 cut(s) 30, 393
DpnI GATC 2 cut(s) 162, 168
DpnII GATC 2 cut(s) 160, 166
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 134
Eco57I CTGAAG 4 cut(s) 123, 474, 489, 525
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 3 cut(s) 370, 385, 530
FaiI YATR 7 cut(s) 153, 174, 368, 383, 404, 521, 528
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FatI CATG 3 cut(s) 366, 381, 526
Fnu4HI GCNGC 2 cut(s) 35, 474
FokI GGATG 2 cut(s) 92, 347
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 35, 474
FspBI CTAG 2 cut(s) 204, 233
GluI GCNGC 2 cut(s) 35, 474
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 2 cut(s) 37, 348
Hin1II CATG 3 cut(s) 370, 385, 530
HinfI GANTC 1 cut(s) 494
Hpy188I TCNGA 2 cut(s) 499, 548
Hpy188III TCNNGA 3 cut(s) 164, 301, 425
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 4 cut(s) 98, 275, 338, 549
HpyCH4V TGCA 1 cut(s) 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 473, 482
HpyF3I CTNAG 2 cut(s) 30, 393
Hsp92II CATG 3 cut(s) 370, 385, 530
Kzo9I GATC 2 cut(s) 160, 166
LmnI GCTCC 3 cut(s) 97, 261, 523
LpnPI CCDG 9 cut(s) 51, 62, 80, 81, 89, 114, 117, 150, 306
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 1 cut(s) 61
MaeI CTAG 2 cut(s) 204, 233
MaeIII GTNAC 3 cut(s) 227, 235, 446
MalI GATC 2 cut(s) 162, 168
MboI GATC 2 cut(s) 160, 166
MboII GAAGA 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 2 cut(s) 180, 431
MnlI CCTC 5 cut(s) 300, 325, 331, 334, 496
MspA1I CMGCKG 2 cut(s) 34, 467
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 43, 473, 482
NdeII GATC 2 cut(s) 160, 166
NlaIII CATG 3 cut(s) 370, 385, 530
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 2 cut(s) 235, 446
PfeI GAWTC 1 cut(s) 494
PkrI GCNGC 2 cut(s) 36, 475
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 134
SatI GCNGC 2 cut(s) 35, 474
Sau3AI GATC 2 cut(s) 160, 166
Sau96I GGNCC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 31
SetI ASST 5 cut(s) 90, 234, 292, 399, 541
SfaNI GCATC 1 cut(s) 61
SinI GGWCC 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 2 cut(s) 180, 431
SsiI CCGC 2 cut(s) 34, 465
SspMI CTAG 2 cut(s) 204, 233
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 349
TaqI TCGA 1 cut(s) 435
TasI AATT 2 cut(s) 180, 431
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 1 cut(s) 494
TscAI CASTG 3 cut(s) 136, 451, 457
TseFI GTSAC 2 cut(s) 235, 446
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 2 cut(s) 235, 446
TspDTI ATGAA 3 cut(s) 168, 444, 543
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 3 cut(s) 136, 451, 457
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 431
XspI CTAG 2 cut(s) 204, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.