Rh6BG306800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001303
Physical Location & Seq
Reverse (-)
53609525 .. 53615325
5801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG306800.1

Sequence Viewer

Length: 1407 bp
ATGGATTCGGCAAGGCAATGGTTCCAGAAGCTTCAGCCGCGCGATAAAGTGAGGAGCTACGGCAGGAAGAAGGACACCGCCGGCGGCGACGACGGCTCCAATACCGACGATGAAGCGCTTTCGAGTGTCACCAAGCAGAAGGCCGCCGCGGCCAAGCAGTACATTGAGAACCATTACAAGGAGCAGATGAAGAATCTCCATGAGAGGAGAGAAAGACGAAATATGTTGGAGAAGAAGTTGGCTGATGCTGATGTGTCTGAGGAGGATCAGAACAACCTCCTCAAGTTCTTGGAGAAGAAAGAAACCGAATACATGCGCCTTCAGAGGCATAAAATGGGTGTCGATGATTTTGAGTTGCTGACTATGATTGGCAAGGGTGCATTTGGTGAGGTTCGTGTCTGCAGGGAAAAGACAACAGGTTCTGTATATGCCATGAAAAAGCTTAAGAAATCAGAAATGCTTCGTAGAGGCCAGGTTGAGCATGTAAAAGCTGAAAGGAATCTGCTTGCTGAGGTGGACAGCAATTGCATTGTCAAGCTCTATTGTTCTTTTCAAGACGATGACTATCTTTATCTTATAATGGAATACTTACCTGGAGGAGATATGATGACTTTACTTATGAGAAAGGATATTTTGACCGAAGATGAGGCTAGATTTTATGTCGCTGAAACAGTTTTGGCTATTGAATCTATCCATAAACACCATTATATTCATAGGGATATTAAGCCTGATAATTTACTACTCGATAGATATGGACACTTGAGACTGTCAGATTTTGGATTATGCAAACCTTTAGACTGCAGTACAATTCATGAAGACTTTTCAATAAATAATGCCAATGGTTCTGGACCCAATGATGAACGTCCGGCAGCTCCAAAACGCACACAACAAGAGCAACTGCAACATTGGCAGAAGAATAGGAGAATGCTTGCTTATTCAACTGTTGGTACACCTGATTATATTGCTCCAGAAGTTCTATTGAAAAAAGGCTATGGAATGGAATGTGATTGGTGGTCACTTGGGGCAATTATGTATGAAATGCTAGTCGGTTACCCGCCCTTCTATTCTGATGATCCAATGTCAACATGCAGGAAGATAGTAAACTGGAAAACTCATTTGAAATTTCCAGAAGAAGCAAAGCTATCTCCAGAGGCAAAAGATCTTATTAGTAAACTCTTGTGTCATGTCAACCAAAGGTTGGGAACAAATGGCGCAAGTGAAATAAAGGTTCATCCTTGGTTTGAAGGTACTGAATGGGAAAAGCTTTATCAAATGGATGCTGCATTTATTCCTGAGGTCAATGATGATCTGGATACTCAAAATTTTGAAAAGTTTGAAGAGGCTGACAACCAAGCTCAGTCTTCAGCCAAAACCGGTCCCTGGAGAAAGGTAAGCTTTGGAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

468

Amino Acids

54.24

Weight (kDa)

6.11

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 118 - 267 3.9e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 118 - 265 2.6e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 311 - 414 2.2e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 578
AccB7I CCANNNNNTGG 1 cut(s) 1198
AccII CGCG 3 cut(s) 40, 42, 149
AciI CCGC 7 cut(s) 38, 78, 84, 144, 147, 149, 1055
AclWI GGATC 2 cut(s) 273, 1067
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 3 cut(s) 1121, 1321, 1401
AcuI CTGAAG 3 cut(s) 17, 305, 1347
AfaI GTAC 4 cut(s) 161, 805, 949, 1249
AfeI AGCGCT 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 178, 1198, 1380
AflII CTTAAG 1 cut(s) 443
AgeI ACCGGT 1 cut(s) 1373
AgsI TTSAA 9 cut(s) 554, 686, 825, 939, 982, 1120, 1244, 1328, 1337
AjnI CCWGG 3 cut(s) 471, 592, 1379
Alw26I GTCTC 1 cut(s) 757
AlwI GGATC 2 cut(s) 273, 1067
AlwNI CAGNNNCTG 1 cut(s) 422
Aor51HI AGCGCT 1 cut(s) 117
AoxI GGCC 3 cut(s) 141, 150, 469
ApeKI GCWGC 2 cut(s) 869, 1280
ApoI RAATTY 3 cut(s) 1121, 1321, 1401
AsiGI ACCGGT 1 cut(s) 1373
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 4 cut(s) 42, 118, 318, 1214
AspS9I GGNCC 2 cut(s) 848, 1376
AsuHPI GGTGA 2 cut(s) 121, 398
AvaII GGWCC 2 cut(s) 848, 1376
AxyI CCTNAGG 1 cut(s) 1293
BbsI GAAGAC 2 cut(s) 822, 1353
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 881, 1267
BceAI ACGGC 2 cut(s) 76, 109
BciT130I CCWGG 3 cut(s) 473, 594, 1381
BciVI GTATCC 1 cut(s) 1306
BcoDI GTCTC 1 cut(s) 757
BfaI CTAG 2 cut(s) 651, 1043
BfmI CTRYAG 2 cut(s) 400, 799
BfoI RGCGCY 1 cut(s) 119
BfrI CTTAAG 1 cut(s) 443
BfuI GTATCC 1 cut(s) 1306
BglI GCCNNNNNGGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 1159
BisI GCNGC 7 cut(s) 38, 85, 144, 147, 150, 870, 1281
BlsI GCNGC 7 cut(s) 39, 86, 145, 148, 151, 871, 1282
Bme1390I CCNGG 3 cut(s) 473, 594, 1381
Bme18I GGWCC 2 cut(s) 848, 1376
BmgT120I GGNCC 2 cut(s) 848, 1376
BmiI GGNNCC 4 cut(s) 23, 97, 850, 1378
BmrFI CCNGG 3 cut(s) 473, 594, 1381
BmsI GCATC 2 cut(s) 235, 1267
BpiI GAAGAC 2 cut(s) 822, 1353
BpmI CTGGAG 4 cut(s) 615, 951, 1131, 1402
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 266, 781
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 147, 1235, 1379
BsaWI WCCGGW 1 cut(s) 1373
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 221, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 1198, 1380
Bse118I RCCGGY 2 cut(s) 80, 1373
Bse1I ACTGG 1 cut(s) 1109
Bse21I CCTNAGG 1 cut(s) 1293
Bse3DI GCAATG 1 cut(s) 23
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 3 cut(s) 473, 594, 1381
BseDI CCNNGG 3 cut(s) 147, 1235, 1379
BseGI GGATG 2 cut(s) 1231, 1282
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 3 cut(s) 178, 1198, 1380
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 4 cut(s) 249, 501, 1284, 1370
BseNI ACTGG 1 cut(s) 1109
BseRI GAGGAG 5 cut(s) 67, 220, 269, 275, 612
BseXI GCAGC 2 cut(s) 881, 1267
Bsh1236I CGCG 3 cut(s) 40, 42, 149
BshFI GGCC 3 cut(s) 143, 152, 471
BshTI ACCGGT 1 cut(s) 1373
BsiSI CCGG 3 cut(s) 81, 866, 1374
BslFI GGGAC 1 cut(s) 1362
BslI CCNNNNNNNGG 3 cut(s) 178, 1198, 1380
BsmAI GTCTC 1 cut(s) 757
BsmFI GGGAC 1 cut(s) 1362
BsmI GAATGC 1 cut(s) 930
BsnI GGCC 3 cut(s) 143, 152, 471
Bsp143I GATC 4 cut(s) 265, 1072, 1159, 1306
BspACI CCGC 7 cut(s) 38, 78, 84, 144, 147, 149, 1055
BspANI GGCC 3 cut(s) 143, 152, 471
BspCNI CTCAG 4 cut(s) 250, 502, 1285, 1369
BspFNI CGCG 3 cut(s) 40, 42, 149
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 4 cut(s) 23, 97, 850, 1378
BspMAI CTGCAG 2 cut(s) 404, 803
BspPI GGATC 2 cut(s) 273, 1067
BspTI CTTAAG 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 23
BsrFI RCCGGY 2 cut(s) 80, 1373
BsrI ACTGG 1 cut(s) 1109
BssAI RCCGGY 2 cut(s) 80, 1373
BssECI CCNNGG 3 cut(s) 147, 1235, 1379
BssMI GATC 4 cut(s) 265, 1072, 1159, 1306
BssT1I CCWWGG 1 cut(s) 1235
Bst2UI CCWGG 3 cut(s) 473, 594, 1381
Bst4CI ACNGT 3 cut(s) 673, 768, 943
Bst6I CTCTTC 1 cut(s) 1332
BstAFI CTTAAG 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 82, 507, 930
BstDEI CTNAG 4 cut(s) 258, 510, 1293, 1356
BstDSI CCRYGG 1 cut(s) 147
BstEII GGTNACC 1 cut(s) 1049
BstF5I GGATG 2 cut(s) 1231, 1282
BstFNI CGCG 3 cut(s) 40, 42, 149
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 4 cut(s) 42, 118, 318, 1214
BstKTI GATC 4 cut(s) 268, 1075, 1162, 1309
BstMAI GTCTC 1 cut(s) 757
BstMBI GATC 4 cut(s) 265, 1072, 1159, 1306
BstMWI GCNNNNNNNGC 4 cut(s) 37, 93, 149, 907
BstNI CCWGG 3 cut(s) 473, 594, 1381
BstNSI RCATGY 3 cut(s) 316, 485, 1089
BstPI GGTNACC 1 cut(s) 1049
BstSCI CCNGG 3 cut(s) 471, 592, 1379
BstSFI CTRYAG 2 cut(s) 400, 799
BstUI CGCG 3 cut(s) 40, 42, 149
BstV1I GCAGC 2 cut(s) 881, 1267
BstV2I GAAGAC 2 cut(s) 822, 1353
BstX2I RGATCY 1 cut(s) 1159
BstYI RGATCY 1 cut(s) 1159
Bsu36I CCTNAGG 1 cut(s) 1293
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 3 cut(s) 143, 152, 471
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 1231, 1282
Cac8I GCNNGC 3 cut(s) 82, 507, 930
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 1 cut(s) 811
CfoI GCGC 4 cut(s) 42, 118, 318, 1214
Cfr10I RCCGGY 2 cut(s) 80, 1373
Cfr13I GGNCC 2 cut(s) 848, 1376
Cfr42I CCGCGG 1 cut(s) 150
Csp6I GTAC 4 cut(s) 160, 804, 948, 1248
CspAI ACCGGT 1 cut(s) 1373
CviAII CATG 7 cut(s) 200, 313, 433, 482, 812, 1086, 1184
CviQI GTAC 4 cut(s) 160, 804, 948, 1248
DdeI CTNAG 4 cut(s) 258, 510, 1293, 1356
DpnI GATC 4 cut(s) 267, 1074, 1161, 1308
DpnII GATC 4 cut(s) 265, 1072, 1159, 1306
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 1332
EarI CTCTTC 1 cut(s) 1332
Eco130I CCWWGG 1 cut(s) 1235
Eco47I GGWCC 2 cut(s) 848, 1376
Eco47III AGCGCT 1 cut(s) 117
Eco57I CTGAAG 3 cut(s) 17, 305, 1347
Eco81I CCTNAGG 1 cut(s) 1293
Eco91I GGTNACC 1 cut(s) 1049
EcoO65I GGTNACC 1 cut(s) 1049
EcoRII CCWGG 3 cut(s) 471, 592, 1379
EcoT14I CCWWGG 1 cut(s) 1235
ErhI CCWWGG 1 cut(s) 1235
FaeI CATG 7 cut(s) 203, 316, 436, 485, 815, 1089, 1187
FalI AAGNNNNNCTT 1 cut(s) 1379
FaqI GGGAC 1 cut(s) 1362
FatI CATG 7 cut(s) 199, 312, 432, 481, 811, 1085, 1183
FauI CCCGC 1 cut(s) 1062
Fnu4HI GCNGC 7 cut(s) 38, 85, 144, 147, 150, 870, 1281
FokI GGATG 2 cut(s) 1218, 1289
Fsp4HI GCNGC 7 cut(s) 38, 85, 144, 147, 150, 870, 1281
FspBI CTAG 2 cut(s) 651, 1043
GlaI GCGC 4 cut(s) 41, 117, 317, 1213
GluI GCNGC 7 cut(s) 38, 85, 144, 147, 150, 870, 1281
GsuI CTGGAG 4 cut(s) 615, 951, 1131, 1402
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 3 cut(s) 143, 152, 471
HapII CCGG 3 cut(s) 81, 866, 1374
HhaI GCGC 4 cut(s) 42, 118, 318, 1214
Hin1II CATG 7 cut(s) 203, 316, 436, 485, 815, 1089, 1187
Hin6I GCGC 4 cut(s) 40, 116, 316, 1212
HinP1I GCGC 4 cut(s) 40, 116, 316, 1212
HincII GTYRAC 2 cut(s) 1083, 1189
HindII GTYRAC 2 cut(s) 1083, 1189
HindIII AAGCTT 4 cut(s) 29, 440, 1262, 1393
HinfI GANTC 4 cut(s) 5, 193, 499, 686
HpaII CCGG 3 cut(s) 81, 866, 1374
HphI GGTGA 2 cut(s) 121, 398
Hpy166II GTNNAC 6 cut(s) 517, 950, 1083, 1102, 1172, 1189
Hpy188I TCNGA 6 cut(s) 259, 270, 324, 454, 772, 1069
Hpy188III TCNNGA 9 cut(s) 25, 554, 812, 846, 968, 1127, 1148, 1292, 1310
Hpy8I GTNNAC 6 cut(s) 517, 950, 1083, 1102, 1172, 1189
Hpy99I CGWCG 3 cut(s) 92, 95, 110
HpyAV CCTTC 5 cut(s) 64, 133, 329, 1069, 1238
HpyCH4III ACNGT 3 cut(s) 673, 768, 943
HpyCH4IV ACGT 1 cut(s) 862
HpyCH4V TGCA 8 cut(s) 380, 402, 528, 786, 801, 901, 1089, 1283
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 93, 149, 907
HpyF3I CTNAG 4 cut(s) 258, 510, 1293, 1356
HpySE526I ACGT 1 cut(s) 862
Hsp92II CATG 7 cut(s) 203, 316, 436, 485, 815, 1089, 1187
HspAI GCGC 4 cut(s) 40, 116, 316, 1212
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 4 cut(s) 265, 1072, 1159, 1306
LmnI GCTCC 5 cut(s) 54, 101, 181, 877, 970
Lsp1109I GCAGC 2 cut(s) 881, 1267
LweI GCATC 2 cut(s) 235, 1267
MaeI CTAG 2 cut(s) 651, 1043
MaeII ACGT 1 cut(s) 862
MaeIII GTNAC 3 cut(s) 127, 1014, 1049
MalI GATC 4 cut(s) 267, 1074, 1161, 1308
MboI GATC 4 cut(s) 265, 1072, 1159, 1306
MfeI CAATTG 1 cut(s) 523
MflI RGATCY 1 cut(s) 1159
MluCI AATT 7 cut(s) 523, 733, 807, 1026, 1121, 1321, 1401
MmeI TCCRAC 1 cut(s) 207
MreI CGCCGGCG 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 3 cut(s) 444, 723, 1405
MspA1I CMGCKG 1 cut(s) 149
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 3 cut(s) 81, 866, 1374
MspR9I CCNGG 3 cut(s) 473, 594, 1381
MunI CAATTG 1 cut(s) 523
Mva1269I GAATGC 1 cut(s) 930
MvaI CCWGG 3 cut(s) 473, 594, 1381
MvnI CGCG 3 cut(s) 40, 42, 149
MwoI GCNNNNNNNGC 4 cut(s) 37, 93, 149, 907
NaeI GCCGGC 1 cut(s) 82
NdeII GATC 4 cut(s) 265, 1072, 1159, 1306
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 7 cut(s) 203, 316, 436, 485, 815, 1089, 1187
NlaIV GGNNCC 4 cut(s) 23, 97, 850, 1378
NmuCI GTSAC 2 cut(s) 127, 1014
NspI RCATGY 3 cut(s) 316, 485, 1089
PagI TCATGA 1 cut(s) 811
PctI GAATGC 1 cut(s) 930
PdiI GCCGGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 4 cut(s) 5, 193, 499, 686
PflMI CCANNNNNTGG 1 cut(s) 1198
PinAI ACCGGT 1 cut(s) 1373
PkrI GCNGC 7 cut(s) 39, 86, 145, 148, 151, 871, 1282
PsiI TTATAA 1 cut(s) 578
Psp6I CCWGG 3 cut(s) 471, 592, 1379
PspEI GGTNACC 1 cut(s) 1049
PspGI CCWGG 3 cut(s) 471, 592, 1379
PspN4I GGNNCC 4 cut(s) 23, 97, 850, 1378
PspPI GGNCC 2 cut(s) 848, 1376
PstI CTGCAG 2 cut(s) 404, 803
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 1159
RsaI GTAC 4 cut(s) 161, 805, 949, 1249
RsaNI GTAC 4 cut(s) 160, 804, 948, 1248
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 444, 723, 1405
SatI GCNGC 7 cut(s) 38, 85, 144, 147, 150, 870, 1281
Sau3AI GATC 4 cut(s) 265, 1072, 1159, 1306
Sau96I GGNCC 2 cut(s) 848, 1376
ScrFI CCNGG 3 cut(s) 473, 594, 1381
SfaNI GCATC 2 cut(s) 235, 1267
SfcI CTRYAG 2 cut(s) 400, 799
SfiI GGCCNNNNNGGCC 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 150
SgrAI CRCCGGYG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 2 cut(s) 848, 1376
SmlI CTYRAG 3 cut(s) 281, 443, 760
SmoI CTYRAG 3 cut(s) 281, 443, 760
Sse9I AATT 7 cut(s) 523, 733, 807, 1026, 1121, 1321, 1401
SsiI CCGC 7 cut(s) 38, 78, 84, 144, 147, 149, 1055
SspMI CTAG 2 cut(s) 651, 1043
StyD4I CCNGG 3 cut(s) 471, 592, 1379
StyI CCWWGG 1 cut(s) 1235
TaaI ACNGT 3 cut(s) 673, 768, 943
TaiI ACGT 1 cut(s) 865
TaqI TCGA 3 cut(s) 122, 342, 744
TaqII GACCGA 1 cut(s) 653
TasI AATT 7 cut(s) 523, 733, 807, 1026, 1121, 1321, 1401
TatI WGTACW 2 cut(s) 159, 803
TauI GCSGC 5 cut(s) 40, 87, 146, 149, 152
TfiI GAWTC 4 cut(s) 5, 193, 499, 686
Tru1I TTAA 3 cut(s) 444, 723, 1405
Tru9I TTAA 3 cut(s) 444, 723, 1405
TseFI GTSAC 2 cut(s) 127, 1014
TseI GCWGC 2 cut(s) 869, 1280
Tsp45I GTSAC 2 cut(s) 127, 1014
TspDTI ATGAA 9 cut(s) 126, 203, 449, 701, 800, 828, 873, 1050, 1220
Van91I CCANNNNNTGG 1 cut(s) 1198
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 848, 1376
XapI RAATTY 3 cut(s) 1121, 1321, 1401
XceI RCATGY 3 cut(s) 316, 485, 1089
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 2 cut(s) 651, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.