MD10G1181100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001303
Physical Location & Seq
Reverse (-)
27423183 .. 27427027
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1181100.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGTTGAGTGCTGACAAGGTTGCTCTTGGTTGTGTTTTCTTTGGTGGTTTTCGGAAATTAGGAAAAATACGAAATATGTTAGAGAAAAAGTTGGCTGATGCCGACGTCTCTGAGGAAGATCAAAGCAACCTTCTGAAGTTCTTGGAGAAGAAAGAAACCGAATACATGCGCCTTCAGAGGCATAAAATGGGTGTTGATGATTTCGACTTGTTGACTATGATCGGCAAGGGAGCATTTGGTGAGGTTCGTGTATGCAGGGAAAAGACGACTGGGTCTGTATATGCCATGAAAAAGCTAAAGAAATCAGAGATGCTTCGTAGAGGCCAGGTAGAGCATGTCAAAGCAGAAAGGAACTTACTTGCTGAGGTGGACAGCAATTGCATTGTCAAGCTGTACTGTTCATTTCAAGATGATGACTATCTTTATCTTATAATGGAATACTTACCGGGAGGAGATATGATGACTTTGCTTATGAGAAAGGATATTTTGACTGAAGATGAAGCTAGATTCTATGTTGCCGAAACAGTTTTGGCTATTGAGTCTATCCATAAACACAATTATGTTCATAGGGATATTAAGCCTGACAATTTGCTACTCGATAGATATGGACACTTGAGACTGTCAGATTTTGGGTTGTGTAAACCTTTAGACTGCAGTACTATCCGAGAAGATTTTTCAGTAGCACCCAATGCTAATGGCGCTGCACAGAATGATGAACATCCTGCGGCTCCAAAACGAACACAACAAGAGCAACTGCAACATTGGCAGAAGAATAGGAGAATGCTTGCTTATTCGACTGTTGGTACCCCTGACTATATTGCTCCAGAAGTTCTGTTGAAAAAAGGTTATGGGATGGAATGCGATTGGTGGTCACTTGGTGCTATTATGTATGAAATGCTTGTGGGTTACCCACCCTTCTATTCTGATGAGCCAATGTCAACATGCAGGAAGATTGTAAACTGGAAAACTCATTTGAAATTTCCAGAAGAAGCAATGCTATCTCCAGGGGCAAAAGATCTTATTAGTAAACTTTTGTGTAATGTCAACCTAAGGTTGGGAATAAACGGCGCAGATGAAATAAAGGTTCATCATTGGTTCGATGGGACTGAGTGGGATAAGCTTTATCAAATGGATGCTGCATTTATTCCTGAGGTCAATGATGATTTGGATACTCAAAATTTCGAAAAGTTCGATGAGGTTGACTATCAAACTCAGAGTTCATCCAAAACTGGTCCCTGGAGAAAGATGCTCTCTTCTAAGGACATTAATTTTGTGGGCTATACCTATAAAAACTTCGAAATTGTTAACGATTATCAAGTGCCTGGGATGGCCTCGTTGAAGAAGAAAAACGCCAAATCGAAAAGACCATCTGTAAAAACACTTTTTGATGAAGAGTCTGAGACGTCTGAGACATCAGAAACTTCCGATAGCGGAAGTAGTCACCATGTGCAAGGGAGCTTTTTGAACCTCTTGCCTCCCCAGTTAGAAGTATCTCAAAACCGGGAGGAGTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

506

Amino Acids

57.9

Weight (kDa)

5.66

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 70 - 220 3.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 70 - 216 3.7e-21 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 142 - 222 8.9e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 263 - 366 3.5e-20 Protein kinase domain
Pkinase_C PF00433 385 - 429 9.3e-08 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AasI GACNNNNNNGTC 1 cut(s) 271
AatII GACGTC 2 cut(s) 108, 1406
Acc65I GGTACC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 803
AciI CCGC 2 cut(s) 725, 1431
AcsI RAATTY 2 cut(s) 977, 1177
AcuI CTGAAG 3 cut(s) 155, 158, 513
AcyI GRCGYC 2 cut(s) 105, 1403
AdeI CACNNNGTG 2 cut(s) 878, 1447
AfaI GTAC 3 cut(s) 395, 658, 805
AfiI CCNNNNNNNGG 1 cut(s) 1054
AgsI TTSAA 5 cut(s) 407, 838, 976, 1339, 1465
AjnI CCWGG 4 cut(s) 324, 1003, 1235, 1321
AluBI AGCT 5 cut(s) 295, 391, 503, 1120, 1458
AluI AGCT 5 cut(s) 295, 391, 503, 1120, 1458
Alw26I GTCTC 4 cut(s) 112, 610, 1394, 1403
AoxI GGCC 2 cut(s) 322, 1329
ApeKI GCWGC 2 cut(s) 701, 1136
ApoI RAATTY 2 cut(s) 977, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 1253, 1285
AseI ATTAAT 1 cut(s) 1266
Asp718I GGTACC 1 cut(s) 803
AspLEI GCGC 3 cut(s) 171, 701, 1070
AspS9I GGNCC 1 cut(s) 1232
AsuC2I CCSGG 2 cut(s) 447, 1502
AsuHPI GGTGA 2 cut(s) 251, 1433
AsuII TTCGAA 2 cut(s) 1182, 1296
AvaII GGWCC 1 cut(s) 1232
AxyI CCTNAGG 2 cut(s) 1049, 1149
BanI GGYRCC 1 cut(s) 803
BbsI GAAGAC 1 cut(s) 1503
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 688, 1123
BccI CCATC 4 cut(s) 847, 1094, 1321, 1375
BceAI ACGGC 1 cut(s) 1081
BciT130I CCWGG 4 cut(s) 326, 1005, 1237, 1323
BciVI GTATCC 1 cut(s) 1162
BcnI CCSGG 2 cut(s) 447, 1502
BcoDI GTCTC 4 cut(s) 112, 610, 1394, 1403
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 1 cut(s) 652
BfoI RGCGCY 1 cut(s) 702
BfuI GTATCC 1 cut(s) 1162
BglII AGATCT 1 cut(s) 1015
BisI GCNGC 3 cut(s) 702, 726, 1137
BlsI GCNGC 3 cut(s) 703, 727, 1138
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 6 cut(s) 326, 447, 1005, 1237, 1323, 1502
Bme18I GGWCC 1 cut(s) 1232
BmgT120I GGNCC 1 cut(s) 1232
BmiI GGNNCC 3 cut(s) 729, 805, 1234
BmrFI CCNGG 6 cut(s) 326, 447, 1005, 1237, 1323, 1502
BmrI ACTGGG 2 cut(s) 279, 1474
BmsI GCATC 4 cut(s) 88, 300, 1123, 1236
BmuI ACTGGG 2 cut(s) 279, 1474
BpiI GAAGAC 1 cut(s) 1503
BpmI CTGGAG 3 cut(s) 807, 987, 1258
Bpu10I CCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 2 cut(s) 1182, 1296
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 2 cut(s) 447, 1502
BsaBI GATNNNNATC 2 cut(s) 417, 717
BsaHI GRCGYC 2 cut(s) 105, 1403
BsaJI CCNNGG 3 cut(s) 1004, 1235, 1322
Bsc4I CCNNNNNNNGG 1 cut(s) 1054
Bse1I ACTGG 4 cut(s) 274, 965, 1234, 1480
Bse21I CCTNAGG 2 cut(s) 1049, 1149
Bse3DI GCAATG 1 cut(s) 999
Bse8I GATNNNNATC 2 cut(s) 417, 717
BseBI CCWGG 4 cut(s) 326, 1005, 1237, 1323
BseDI CCNNGG 3 cut(s) 1004, 1235, 1322
BseGI GGATG 5 cut(s) 718, 858, 1138, 1220, 1332
BseJI GATNNNNATC 2 cut(s) 417, 717
BseLI CCNNNNNNNGG 1 cut(s) 1054
BseMI GCAATG 1 cut(s) 999
BseMII CTCAG 7 cut(s) 102, 354, 1098, 1140, 1226, 1389, 1398
BseNI ACTGG 4 cut(s) 274, 965, 1234, 1480
BseRI GAGGAG 1 cut(s) 465
BseXI GCAGC 2 cut(s) 688, 1123
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 2 cut(s) 324, 1331
BshNI GGYRCC 1 cut(s) 803
BsiSI CCGG 2 cut(s) 446, 1501
BslFI GGGAC 2 cut(s) 1117, 1218
BslI CCNNNNNNNGG 1 cut(s) 1054
BsmAI GTCTC 4 cut(s) 112, 610, 1394, 1403
BsmBI CGTCTC 2 cut(s) 112, 1394
BsmFI GGGAC 2 cut(s) 1117, 1218
BsmI GAATGC 2 cut(s) 786, 863
BsnI GGCC 2 cut(s) 324, 1331
Bsp119I TTCGAA 2 cut(s) 1182, 1296
Bsp143I GATC 3 cut(s) 118, 219, 1015
BspACI CCGC 2 cut(s) 725, 1431
BspANI GGCC 2 cut(s) 324, 1331
BspCNI CTCAG 7 cut(s) 103, 355, 1099, 1141, 1225, 1390, 1399
BspLI GGNNCC 3 cut(s) 729, 805, 1234
BspMAI CTGCAG 1 cut(s) 656
BspT104I TTCGAA 2 cut(s) 1182, 1296
BspT107I GGYRCC 1 cut(s) 803
BsrDI GCAATG 1 cut(s) 999
BsrI ACTGG 4 cut(s) 274, 965, 1234, 1480
BssECI CCNNGG 3 cut(s) 1004, 1235, 1322
BssMI GATC 3 cut(s) 118, 219, 1015
BssNI GRCGYC 2 cut(s) 105, 1403
Bst2UI CCWGG 4 cut(s) 326, 1005, 1237, 1323
Bst4CI ACNGT 4 cut(s) 398, 526, 621, 799
Bst6I CTCTTC 2 cut(s) 1258, 1386
BstACI GRCGYC 2 cut(s) 105, 1403
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBI TTCGAA 2 cut(s) 1182, 1296
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 9 cut(s) 111, 363, 1049, 1107, 1149, 1212, 1257, 1398, 1407
BstEII GGTNACC 1 cut(s) 905
BstF5I GGATG 5 cut(s) 718, 858, 1138, 1220, 1332
BstH2I RGCGCY 1 cut(s) 702
BstHHI GCGC 3 cut(s) 171, 701, 1070
BstKTI GATC 3 cut(s) 121, 222, 1018
BstMAI GTCTC 4 cut(s) 112, 610, 1394, 1403
BstMBI GATC 3 cut(s) 118, 219, 1015
BstMWI GCNNNNNNNGC 3 cut(s) 689, 698, 763
BstNI CCWGG 4 cut(s) 326, 1005, 1237, 1323
BstNSI RCATGY 3 cut(s) 169, 338, 945
BstPI GGTNACC 1 cut(s) 905
BstSCI CCNGG 6 cut(s) 324, 445, 1003, 1235, 1321, 1500
BstSFI CTRYAG 1 cut(s) 652
BstV1I GCAGC 2 cut(s) 688, 1123
BstV2I GAAGAC 1 cut(s) 1503
BstX2I RGATCY 1 cut(s) 1015
BstYI RGATCY 1 cut(s) 1015
Bsu36I CCTNAGG 2 cut(s) 1049, 1149
BsuI GTATCC 1 cut(s) 1162
BsuRI GGCC 2 cut(s) 324, 1331
BtsCI GGATG 5 cut(s) 718, 858, 1138, 1220, 1332
Cac8I GCNNGC 1 cut(s) 786
CfoI GCGC 3 cut(s) 171, 701, 1070
Cfr13I GGNCC 1 cut(s) 1232
Csp6I GTAC 3 cut(s) 394, 657, 804
CviAII CATG 5 cut(s) 166, 286, 335, 942, 1445
CviQI GTAC 3 cut(s) 394, 657, 804
DdeI CTNAG 9 cut(s) 111, 363, 1049, 1107, 1149, 1212, 1257, 1398, 1407
DpnI GATC 3 cut(s) 120, 221, 1017
DpnII GATC 3 cut(s) 118, 219, 1015
DraIII CACNNNGTG 2 cut(s) 878, 1447
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 1258, 1386
EarI CTCTTC 2 cut(s) 1258, 1386
Eco47I GGWCC 1 cut(s) 1232
Eco57I CTGAAG 3 cut(s) 155, 158, 513
Eco81I CCTNAGG 2 cut(s) 1049, 1149
Eco91I GGTNACC 1 cut(s) 905
EcoO65I GGTNACC 1 cut(s) 905
EcoRII CCWGG 4 cut(s) 324, 1003, 1235, 1321
Esp3I CGTCTC 2 cut(s) 112, 1394
FaeI CATG 5 cut(s) 169, 289, 338, 945, 1448
FaqI GGGAC 2 cut(s) 1117, 1218
FatI CATG 5 cut(s) 165, 285, 334, 941, 1444
Fnu4HI GCNGC 3 cut(s) 702, 726, 1137
FokI GGATG 5 cut(s) 705, 865, 1145, 1207, 1339
Fsp4HI GCNGC 3 cut(s) 702, 726, 1137
FspBI CTAG 1 cut(s) 504
GlaI GCGC 3 cut(s) 170, 700, 1069
GluI GCNGC 3 cut(s) 702, 726, 1137
GsuI CTGGAG 3 cut(s) 807, 987, 1258
HaeII RGCGCY 1 cut(s) 702
HaeIII GGCC 2 cut(s) 324, 1331
HapII CCGG 2 cut(s) 446, 1501
HhaI GCGC 3 cut(s) 171, 701, 1070
Hin1I GRCGYC 2 cut(s) 105, 1403
Hin1II CATG 5 cut(s) 169, 289, 338, 945, 1448
Hin6I GCGC 3 cut(s) 169, 699, 1068
HinP1I GCGC 3 cut(s) 169, 699, 1068
HincII GTYRAC 5 cut(s) 213, 939, 1045, 1201, 1306
HindII GTYRAC 5 cut(s) 213, 939, 1045, 1201, 1306
HindIII AAGCTT 1 cut(s) 1118
HinfI GANTC 4 cut(s) 507, 539, 1394, 1508
HpaI GTTAAC 1 cut(s) 1306
HpaII CCGG 2 cut(s) 446, 1501
HphI GGTGA 2 cut(s) 251, 1433
Hpy166II GTNNAC 9 cut(s) 213, 370, 641, 939, 958, 1028, 1045, 1201, 1306
Hpy188III TCNNGA 5 cut(s) 407, 824, 983, 1148, 1515
Hpy8I GTNNAC 9 cut(s) 213, 370, 641, 939, 958, 1028, 1045, 1201, 1306
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 140, 182, 925
HpyCH4III ACNGT 4 cut(s) 398, 526, 621, 799
HpyCH4IV ACGT 2 cut(s) 105, 1403
HpyCH4V TGCA 8 cut(s) 255, 381, 654, 704, 757, 945, 1139, 1450
HpyF10VI GCNNNNNNNGC 3 cut(s) 689, 698, 763
HpyF3I CTNAG 9 cut(s) 111, 363, 1049, 1107, 1149, 1212, 1257, 1398, 1407
HpySE526I ACGT 2 cut(s) 105, 1403
Hsp92I GRCGYC 2 cut(s) 105, 1403
Hsp92II CATG 5 cut(s) 169, 289, 338, 945, 1448
HspAI GCGC 3 cut(s) 169, 699, 1068
KpnI GGTACC 1 cut(s) 807
KspAI GTTAAC 1 cut(s) 1306
Kzo9I GATC 3 cut(s) 118, 219, 1015
LmnI GCTCC 4 cut(s) 230, 733, 826, 1455
Lsp1109I GCAGC 2 cut(s) 688, 1123
LweI GCATC 4 cut(s) 88, 300, 1123, 1236
MaeI CTAG 1 cut(s) 504
MaeII ACGT 2 cut(s) 105, 1403
MaeIII GTNAC 3 cut(s) 870, 905, 1439
MalI GATC 3 cut(s) 120, 221, 1017
MboI GATC 3 cut(s) 118, 219, 1015
MfeI CAATTG 1 cut(s) 376
MflI RGATCY 1 cut(s) 1015
MluCI AATT 8 cut(s) 56, 376, 556, 586, 977, 1177, 1267, 1299
MlyI GAGTC 3 cut(s) 548, 1403, 1517
MseI TTAA 3 cut(s) 576, 1266, 1305
MslI CAYNNNNRTG 1 cut(s) 558
MspI CCGG 2 cut(s) 446, 1501
MspR9I CCNGG 6 cut(s) 326, 447, 1005, 1237, 1323, 1502
MunI CAATTG 1 cut(s) 376
Mva1269I GAATGC 2 cut(s) 786, 863
MvaI CCWGG 4 cut(s) 326, 1005, 1237, 1323
MwoI GCNNNNNNNGC 3 cut(s) 689, 698, 763
NciI CCSGG 2 cut(s) 447, 1502
NdeII GATC 3 cut(s) 118, 219, 1015
NlaIII CATG 5 cut(s) 169, 289, 338, 945, 1448
NlaIV GGNNCC 3 cut(s) 729, 805, 1234
NmuCI GTSAC 2 cut(s) 870, 1439
NspI RCATGY 3 cut(s) 169, 338, 945
NspV TTCGAA 2 cut(s) 1182, 1296
PcsI WCGNNNNNNNCGW 1 cut(s) 1188
PctI GAATGC 2 cut(s) 786, 863
PfeI GAWTC 1 cut(s) 507
PflFI GACNNNGTC 1 cut(s) 271
PkrI GCNGC 3 cut(s) 703, 727, 1138
PleI GAGTC 3 cut(s) 547, 1402, 1516
PpsI GAGTC 3 cut(s) 547, 1402, 1516
PshBI ATTAAT 1 cut(s) 1266
PsiI TTATAA 1 cut(s) 431
Psp6I CCWGG 4 cut(s) 324, 1003, 1235, 1321
PspEI GGTNACC 1 cut(s) 905
PspGI CCWGG 4 cut(s) 324, 1003, 1235, 1321
PspN4I GGNNCC 3 cut(s) 729, 805, 1234
PspPI GGNCC 1 cut(s) 1232
PstI CTGCAG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 1015
PsyI GACNNNGTC 1 cut(s) 271
RsaI GTAC 3 cut(s) 395, 658, 805
RsaNI GTAC 3 cut(s) 394, 657, 804
RseI CAYNNNNRTG 1 cut(s) 558
SaqAI TTAA 3 cut(s) 576, 1266, 1305
SatI GCNGC 3 cut(s) 702, 726, 1137
Sau3AI GATC 3 cut(s) 118, 219, 1015
Sau96I GGNCC 1 cut(s) 1232
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 3 cut(s) 548, 1403, 1517
ScrFI CCNGG 6 cut(s) 326, 447, 1005, 1237, 1323, 1502
SfaNI GCATC 4 cut(s) 88, 300, 1123, 1236
SfcI CTRYAG 1 cut(s) 652
SfuI TTCGAA 2 cut(s) 1182, 1296
SinI GGWCC 1 cut(s) 1232
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 8 cut(s) 56, 376, 556, 586, 977, 1177, 1267, 1299
SsiI CCGC 2 cut(s) 725, 1431
SspMI CTAG 1 cut(s) 504
StyD4I CCNGG 6 cut(s) 324, 445, 1003, 1235, 1321, 1500
TaaI ACNGT 4 cut(s) 398, 526, 621, 799
TaiI ACGT 2 cut(s) 108, 1406
TaqI TCGA 8 cut(s) 204, 597, 794, 1098, 1182, 1191, 1296, 1358
TasI AATT 8 cut(s) 56, 376, 556, 586, 977, 1177, 1267, 1299
TatI WGTACW 2 cut(s) 393, 656
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 1 cut(s) 507
Tru1I TTAA 3 cut(s) 576, 1266, 1305
Tru9I TTAA 3 cut(s) 576, 1266, 1305
TseFI GTSAC 2 cut(s) 870, 1439
TseI GCWGC 2 cut(s) 701, 1136
Tsp45I GTSAC 2 cut(s) 870, 1439
Tth111I GACNNNGTC 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 1232
VspI ATTAAT 1 cut(s) 1266
XapI RAATTY 2 cut(s) 977, 1177
XceI RCATGY 3 cut(s) 169, 338, 945
XspI CTAG 1 cut(s) 504
ZraI GACGTC 2 cut(s) 106, 1404
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.