MD05G1265100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001303
Physical Location & Seq
Forward (+)
39996009 .. 40000962
4954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1265100.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGGATTCTGCGAGGAGCTGGCTTAAGAAGTTCCAGCCGAGGGCGAAGAAGAAGGCGGCGGCGGAGGATACGAAGGCCGAGCAGCAGAATTCTGTTTCCGATGAGGCGCCGTCGAGCGTGACCAAACAGAAGGTGGAGGCAGCCAAACAGTACATTGAGAACCACTACAAGGCGCAAATGAAGTCCTTGCAGGAAAGGAAGGAGAGGCGGTGGATGCTCGAAAGGCGGCTCGCCGACGCGGATGTTTCGCAGGAGGATCAGATGAATGTGTTGAAGTATTTGGAGCAGAAGGAGACGGAGTACATGCATCTTCAGAGGCATAAAATGGGGGTTGATGACTTTCAGCTGTTGACCATCATCGGAAGGGGTGCATTCGGAGAGGTCAGAATCTGCAGAGAGAAATCTACTGGGCAGGTCTACGCCATGAAAAAGCTCAAGAAATCGGAGATGCTCCGGAGAGGCCAGGTGGAACATGTTAAAGCTGAAAGAAATCTTCTTGCTGAGGTTGATAGTCCTTACATCGTTAAGCTCTACTGCTCCTTTCAAGATGACGAATTTTTGTATCTTATAATGGAATATCTTCCGGGCGGTGATATGATGACATTACTAATGCGCAAAGATATCTTGACTGAAGATGAAGCCAGGTTTTATGTAGGGGAGACAGTCCTTGCCATTGAGTCTATTCATAAACACAACTACATCCACAGGGATATTAAGCCTGATAATTTGCTGCTTGATCGTTATGGCCACATGAAGCTTTCAGATTTTGGGTTGTGTAAGCCTTTGGGATCTAACAGCTTTCCAGATCTTAGTGAGAATGGTGAGAATGGCAATGCAGTTGGAGGGAATTCAAAATCCCCTTCAGCGAGTCATAAACATTCTAACCTTCCCGCAACACCAAAAAGGACACAGCAGGAACAGTTATTGCACTGGCAAAAGAACAGGCGAATGCTGGCTTATTCAACAGTTGGAACTCCAGATTACATTGCTCCAGAAGTATTGCTGAAGAAAGGATATGGAATGGAATGTGACTGGTGGTCTCTTGGTGCAATCATGTATGAGATGCTTGTAGGATTTCCACCTTTCTATTCTGACGAACCAATGTCAACATGCAGAAAGATTGTTAACTGGAGAACTCATCTGAAATTCCCAGGGGAAGCAAAGCTCTCTTCTGAAGCTAAGGATCTCATTTGCAAGCTCCTCTGCAATGTTGAGCAGAGGCTTGGGACGCAAGGAGCTCATGAAATAAAAGCACACCCATGGTTTGATGGGTTACAATGGGATAGATTATATCAGATGGAAGCGGCTTTCATACCAGAGGTCAACAATGAGTTAGATACTCAAAACTTTGAGAAGTTTGATGAGTTGGGGGCTCCAGTAGAGACTTCAACAAAATCTGGTCCGTGGAGAAAGATGCTTTCCTCCACGGATACAAATTTCGTCGGCTATACCTACAAGAATTTTGAAATTGTTGATGAGCATCATATGCCTGGCATTGCTGAGTTGAAGAAAAAGAATACCAAGCCAAAACGACCATCTGTTAAATCATTATTTGACACACCTGATCCTCCGGACTCACCAATTAACGGAACCCCTCCCTTACATGTGCCCAACCAGTTGGGTGTCTCAGGAGGCTCTCAGCCTTCACGGCAATCTACTAGACCCCCACTACCTCAACACAAACCGCCACGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

566

Amino Acids

64.78

Weight (kDa)

8.53

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 115 - 266 2.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 116 - 262 5.8e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 319 - 422 7.8e-22 Protein kinase domain
Pkinase_C PF00433 441 - 485 2.1e-07 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
Acc16I TGCGCA 1 cut(s) 614
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 239
AccIII TCCGGA 2 cut(s) 453, 1570
AclWI GGATC 4 cut(s) 264, 796, 1191, 1559
AcoI YGGCCR 1 cut(s) 745
AcsI RAATTY 6 cut(s) 88, 554, 847, 1145, 1435, 1459
AcuI CTGAAG 5 cut(s) 296, 651, 846, 1025, 1194
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 2 cut(s) 152, 302
AfiI CCNNNNNNNGG 3 cut(s) 40, 169, 1586
AflII CTTAAG 1 cut(s) 23
AflIII ACRYGT 2 cut(s) 472, 1603
AgsI TTSAA 7 cut(s) 274, 545, 852, 963, 1389, 1466, 1507
AhdI GACNNNNNGTC 1 cut(s) 1036
AjnI CCWGG 4 cut(s) 462, 641, 1150, 1489
Alw21I GWGCWC 1 cut(s) 1240
Alw26I GTCTC 5 cut(s) 287, 653, 1044, 1376, 1630
AlwI GGATC 4 cut(s) 264, 796, 1191, 1559
AlwNI CAGNNNCTG 1 cut(s) 390
Aor13HI TCCGGA 2 cut(s) 453, 1570
AoxI GGCC 3 cut(s) 75, 460, 745
ApeKI GCWGC 3 cut(s) 82, 140, 730
ApoI RAATTY 6 cut(s) 88, 554, 847, 1145, 1435, 1459
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 3 cut(s) 109, 175, 615
AspS9I GGNCC 1 cut(s) 1400
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 3 cut(s) 602, 833, 1569
AvaII GGWCC 1 cut(s) 1400
BaeGI GKGCMC 1 cut(s) 1611
BalI TGGCCA 1 cut(s) 747
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 2 cut(s) 1240, 1375
Bbv12I GWGCWC 1 cut(s) 1240
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 94, 152, 717
BccI CCATC 4 cut(s) 362, 1262, 1291, 1543
BceAI ACGGC 2 cut(s) 94, 1664
BciT130I CCWGG 4 cut(s) 464, 643, 1152, 1491
BciVI GTATCC 2 cut(s) 61, 1423
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 5 cut(s) 287, 653, 1044, 1376, 1630
BfaI CTAG 1 cut(s) 1659
BfmI CTRYAG 1 cut(s) 391
BfoI RGCGCY 1 cut(s) 110
BfrI CTTAAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 403
BfuI GTATCC 2 cut(s) 61, 1423
BglI GCCNNNNNGGC 1 cut(s) 1648
BglII AGATCT 1 cut(s) 805
BisI GCNGC 7 cut(s) 57, 60, 83, 141, 227, 731, 1305
BlsI GCNGC 7 cut(s) 58, 61, 84, 142, 228, 732, 1306
Bme1390I CCNGG 5 cut(s) 464, 585, 643, 1152, 1491
Bme18I GGWCC 1 cut(s) 1400
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmgT120I GGNCC 1 cut(s) 1400
BmiI GGNNCC 3 cut(s) 108, 1374, 1591
BmrFI CCNGG 5 cut(s) 464, 585, 643, 1152, 1491
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 6 cut(s) 204, 316, 438, 1053, 1404, 1489
BmuI ACTGGG 1 cut(s) 417
BpmI CTGGAG 4 cut(s) 960, 975, 1150, 1359
Bpu10I CCTNAGC 2 cut(s) 501, 1179
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 1479
BsaHI GRCGYC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 1044
BsaJI CCNNGG 6 cut(s) 38, 1150, 1151, 1259, 1403, 1425
BsaWI WCCGGW 2 cut(s) 453, 1570
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 284, 314
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 169, 1586
Bse1I ACTGG 6 cut(s) 412, 935, 1037, 1133, 1376, 1615
Bse3DI GCAATG 4 cut(s) 838, 984, 1213, 1494
Bse8I GATNNNNATC 1 cut(s) 1479
BseAI TCCGGA 2 cut(s) 453, 1570
BseBI CCWGG 4 cut(s) 464, 643, 1152, 1491
BseDI CCNNGG 6 cut(s) 38, 1150, 1151, 1259, 1403, 1425
BseGI GGATG 3 cut(s) 219, 247, 699
BseJI GATNNNNATC 1 cut(s) 1479
BseLI CCNNNNNNNGG 3 cut(s) 40, 169, 1586
BseMI GCAATG 4 cut(s) 838, 984, 1213, 1494
BseMII CTCAG 4 cut(s) 492, 1491, 1641, 1652
BseNI ACTGG 6 cut(s) 412, 935, 1037, 1133, 1376, 1615
BseRI GAGGAG 2 cut(s) 28, 1190
BseSI GKGCMC 1 cut(s) 1611
BseXI GCAGC 3 cut(s) 94, 152, 717
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 3 cut(s) 77, 462, 747
BshNI GGYRCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 1240
BsiSI CCGG 3 cut(s) 454, 584, 1571
BslFI GGGAC 1 cut(s) 1240
BslI CCNNNNNNNGG 3 cut(s) 40, 169, 1586
BsmAI GTCTC 5 cut(s) 287, 653, 1044, 1376, 1630
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 1240
BsmI GAATGC 2 cut(s) 371, 954
BsnI GGCC 3 cut(s) 77, 462, 747
Bso31I GGTCTC 1 cut(s) 1044
Bsp1286I GDGCHC 3 cut(s) 1240, 1375, 1611
Bsp13I TCCGGA 2 cut(s) 453, 1570
Bsp143I GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
Bsp19I CCATGG 1 cut(s) 1259
BspANI GGCC 3 cut(s) 77, 462, 747
BspCNI CTCAG 4 cut(s) 493, 1492, 1640, 1651
BspEI TCCGGA 2 cut(s) 453, 1570
BspFNI CGCG 1 cut(s) 239
BspHI TCATGA 1 cut(s) 1240
BspLI GGNNCC 3 cut(s) 108, 1374, 1591
BspMAI CTGCAG 1 cut(s) 395
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 4 cut(s) 264, 796, 1191, 1559
BspT107I GGYRCC 1 cut(s) 106
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 1044
BsrDI GCAATG 4 cut(s) 838, 984, 1213, 1494
BsrI ACTGG 6 cut(s) 412, 935, 1037, 1133, 1376, 1615
BssECI CCNNGG 6 cut(s) 38, 1150, 1151, 1259, 1403, 1425
BssMI GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 1259
Bst2UI CCWGG 4 cut(s) 464, 643, 1152, 1491
Bst4CI ACNGT 4 cut(s) 150, 664, 921, 967
Bst6I CTCTTC 1 cut(s) 1174
BstACI GRCGYC 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstC8I GCNNGC 4 cut(s) 20, 231, 954, 1196
BstDEI CTNAG 6 cut(s) 501, 809, 1179, 1500, 1627, 1638
BstDSI CCRYGG 3 cut(s) 1259, 1403, 1425
BstF5I GGATG 3 cut(s) 219, 247, 699
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 3 cut(s) 109, 175, 615
BstKTI GATC 6 cut(s) 259, 739, 791, 808, 1186, 1567
BstMAI GTCTC 5 cut(s) 287, 653, 1044, 1376, 1630
BstMBI GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
BstMWI GCNNNNNNNGC 5 cut(s) 214, 223, 1228, 1486, 1648
BstNI CCWGG 4 cut(s) 464, 643, 1152, 1491
BstNSI RCATGY 4 cut(s) 307, 476, 1113, 1607
BstSCI CCNGG 5 cut(s) 462, 583, 641, 1150, 1489
BstSFI CTRYAG 1 cut(s) 391
BstSLI GKGCMC 1 cut(s) 1611
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 3 cut(s) 94, 152, 717
BstX2I RGATCY 3 cut(s) 788, 805, 1183
BstXI CCANNNNNNTGG 1 cut(s) 1618
BstYI RGATCY 3 cut(s) 788, 805, 1183
BsuI GTATCC 2 cut(s) 61, 1423
BsuRI GGCC 3 cut(s) 77, 462, 747
BtgI CCRYGG 3 cut(s) 1259, 1403, 1425
BtsCI GGATG 3 cut(s) 219, 247, 699
BtsIMutI CAGTG 1 cut(s) 928
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 4 cut(s) 20, 231, 954, 1196
CaiI CAGNNNCTG 1 cut(s) 390
CciI TCATGA 1 cut(s) 1240
CfoI GCGC 3 cut(s) 109, 175, 615
Cfr13I GGNCC 1 cut(s) 1400
CseI GACGC 2 cut(s) 245, 1237
Csp6I GTAC 2 cut(s) 151, 301
CviAII CATG 9 cut(s) 304, 424, 473, 751, 1054, 1110, 1241, 1260, 1604
CviQI GTAC 2 cut(s) 151, 301
DdeI CTNAG 6 cut(s) 501, 809, 1179, 1500, 1627, 1638
DinI GGCGCC 1 cut(s) 108
DpnI GATC 6 cut(s) 258, 738, 790, 807, 1185, 1566
DpnII GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
DriI GACNNNNNGTC 1 cut(s) 1036
EaeI YGGCCR 1 cut(s) 745
Eam1104I CTCTTC 1 cut(s) 1174
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 1 cut(s) 1174
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 1238
Eco130I CCWWGG 1 cut(s) 1259
Eco24I GRGCYC 2 cut(s) 1240, 1375
Eco31I GGTCTC 1 cut(s) 1044
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 1400
Eco53kI GAGCTC 1 cut(s) 1238
Eco57I CTGAAG 5 cut(s) 296, 651, 846, 1025, 1194
EcoICRI GAGCTC 1 cut(s) 1238
EcoRI GAATTC 2 cut(s) 88, 847
EcoRII CCWGG 4 cut(s) 462, 641, 1150, 1489
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 1259
EcoT22I ATGCAT 1 cut(s) 309
EcoT38I GRGCYC 2 cut(s) 1240, 1375
EgeI GGCGCC 1 cut(s) 108
EheI GGCGCC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 1259
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 9 cut(s) 307, 427, 476, 754, 1057, 1113, 1244, 1263, 1607
FaqI GGGAC 1 cut(s) 1240
FatI CATG 9 cut(s) 303, 423, 472, 750, 1053, 1109, 1240, 1259, 1603
FauI CCCGC 1 cut(s) 898
FauNDI CATATG 1 cut(s) 1485
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 7 cut(s) 57, 60, 83, 141, 227, 731, 1305
FokI GGATG 3 cut(s) 226, 254, 686
FriOI GRGCYC 2 cut(s) 1240, 1375
Fsp4HI GCNGC 7 cut(s) 57, 60, 83, 141, 227, 731, 1305
FspBI CTAG 1 cut(s) 1659
FspI TGCGCA 1 cut(s) 614
GlaI GCGC 3 cut(s) 108, 174, 614
GluI GCNGC 7 cut(s) 57, 60, 83, 141, 227, 731, 1305
GsuI CTGGAG 4 cut(s) 960, 975, 1150, 1359
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 3 cut(s) 77, 462, 747
HapII CCGG 3 cut(s) 454, 584, 1571
HgaI GACGC 2 cut(s) 245, 1237
HhaI GCGC 3 cut(s) 109, 175, 615
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 9 cut(s) 307, 427, 476, 754, 1057, 1113, 1244, 1263, 1607
Hin6I GCGC 3 cut(s) 107, 173, 613
HinP1I GCGC 3 cut(s) 107, 173, 613
HincII GTYRAC 4 cut(s) 351, 1107, 1126, 1324
HindII GTYRAC 4 cut(s) 351, 1107, 1126, 1324
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 5 cut(s) 5, 387, 677, 868, 1574
HpaI GTTAAC 1 cut(s) 1126
HpaII CCGG 3 cut(s) 454, 584, 1571
HphI GGTGA 3 cut(s) 602, 833, 1569
Hpy166II GTNNAC 5 cut(s) 351, 418, 1107, 1126, 1324
Hpy8I GTNNAC 5 cut(s) 351, 418, 1107, 1126, 1324
Hpy99I CGWCG 3 cut(s) 115, 239, 1445
HpyAV CCTTC 9 cut(s) 46, 67, 124, 193, 283, 357, 870, 896, 1653
HpyCH4III ACNGT 4 cut(s) 150, 664, 921, 967
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 223, 1228, 1486, 1648
HpyF3I CTNAG 6 cut(s) 501, 809, 1179, 1500, 1627, 1638
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 9 cut(s) 307, 427, 476, 754, 1057, 1113, 1244, 1263, 1607
HspAI GCGC 3 cut(s) 107, 173, 613
KasI GGCGCC 1 cut(s) 106
Kpn2I TCCGGA 2 cut(s) 453, 1570
KspAI GTTAAC 1 cut(s) 1126
Kzo9I GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
LmnI GCTCC 8 cut(s) 15, 283, 456, 542, 994, 1203, 1235, 1378
Lsp1109I GCAGC 3 cut(s) 94, 152, 717
LweI GCATC 6 cut(s) 204, 316, 438, 1053, 1404, 1489
MaeI CTAG 1 cut(s) 1659
MaeIII GTNAC 3 cut(s) 118, 1028, 1272
MalI GATC 6 cut(s) 258, 738, 790, 807, 1185, 1566
MboI GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
MboII GAAGA 9 cut(s) 58, 61, 302, 485, 572, 644, 1018, 1161, 1519
MflI RGATCY 3 cut(s) 788, 805, 1183
MhlI GDGCHC 3 cut(s) 1240, 1375, 1611
MlsI TGGCCA 1 cut(s) 747
MluCI AATT 9 cut(s) 88, 554, 724, 847, 1145, 1435, 1459, 1467, 1581
MluNI TGGCCA 1 cut(s) 747
Mly113I GGCGCC 1 cut(s) 107
MlyI GAGTC 3 cut(s) 686, 877, 1568
MmeI TCCRAC 2 cut(s) 820, 949
Mox20I TGGCCA 1 cut(s) 747
Mph1103I ATGCAT 1 cut(s) 309
MroI TCCGGA 2 cut(s) 453, 1570
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 1 cut(s) 747
MseI TTAA 7 cut(s) 24, 477, 525, 714, 1125, 1542, 1584
MslI CAYNNNNRTG 1 cut(s) 1258
Msp20I TGGCCA 1 cut(s) 747
MspA1I CMGCKG 1 cut(s) 346
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 3 cut(s) 454, 584, 1571
MspR9I CCNGG 5 cut(s) 464, 585, 643, 1152, 1491
Mva1269I GAATGC 2 cut(s) 371, 954
MvaI CCWGG 4 cut(s) 464, 643, 1152, 1491
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 5 cut(s) 214, 223, 1228, 1486, 1648
NarI GGCGCC 1 cut(s) 107
NciI CCSGG 1 cut(s) 585
NcoI CCATGG 1 cut(s) 1259
NdeI CATATG 1 cut(s) 1485
NdeII GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
NlaIII CATG 9 cut(s) 307, 427, 476, 754, 1057, 1113, 1244, 1263, 1607
NlaIV GGNNCC 3 cut(s) 108, 1374, 1591
NmeAIII GCCGAG 2 cut(s) 63, 103
NmuCI GTSAC 2 cut(s) 118, 1028
NsbI TGCGCA 1 cut(s) 614
NsiI ATGCAT 1 cut(s) 309
NspI RCATGY 4 cut(s) 307, 476, 1113, 1607
PagI TCATGA 1 cut(s) 1240
PasI CCCWGGG 1 cut(s) 1151
PciI ACATGT 2 cut(s) 472, 1603
PctI GAATGC 2 cut(s) 371, 954
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 5, 387
PkrI GCNGC 7 cut(s) 58, 61, 84, 142, 228, 732, 1306
PleI GAGTC 3 cut(s) 685, 876, 1568
PluTI GGCGCC 1 cut(s) 110
PpsI GAGTC 3 cut(s) 685, 876, 1568
PscI ACATGT 2 cut(s) 472, 1603
PsiI TTATAA 1 cut(s) 569
Psp124BI GAGCTC 1 cut(s) 1240
Psp6I CCWGG 4 cut(s) 462, 641, 1150, 1489
PspGI CCWGG 4 cut(s) 462, 641, 1150, 1489
PspN4I GGNNCC 3 cut(s) 108, 1374, 1591
PspPI GGNCC 1 cut(s) 1400
PstI CTGCAG 1 cut(s) 395
PstNI CAGNNNCTG 1 cut(s) 390
PsuI RGATCY 3 cut(s) 788, 805, 1183
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 2 cut(s) 152, 302
RsaNI GTAC 2 cut(s) 151, 301
RseI CAYNNNNRTG 1 cut(s) 1258
SacI GAGCTC 1 cut(s) 1240
SaqAI TTAA 7 cut(s) 24, 477, 525, 714, 1125, 1542, 1584
SatI GCNGC 7 cut(s) 57, 60, 83, 141, 227, 731, 1305
Sau3AI GATC 6 cut(s) 256, 736, 788, 805, 1183, 1564
Sau96I GGNCC 1 cut(s) 1400
SchI GAGTC 3 cut(s) 686, 877, 1568
ScrFI CCNGG 5 cut(s) 464, 585, 643, 1152, 1491
SduI GDGCHC 3 cut(s) 1240, 1375, 1611
SfaNI GCATC 6 cut(s) 204, 316, 438, 1053, 1404, 1489
SfcI CTRYAG 1 cut(s) 391
SfoI GGCGCC 1 cut(s) 108
SinI GGWCC 1 cut(s) 1400
SmiMI CAYNNNNRTG 1 cut(s) 1258
SmlI CTYRAG 2 cut(s) 23, 434
SmoI CTYRAG 2 cut(s) 23, 434
Sse9I AATT 9 cut(s) 88, 554, 724, 847, 1145, 1435, 1459, 1467, 1581
SspDI GGCGCC 1 cut(s) 106
SspMI CTAG 1 cut(s) 1659
SstI GAGCTC 1 cut(s) 1240
StyD4I CCNGG 5 cut(s) 462, 583, 641, 1150, 1489
StyI CCWWGG 1 cut(s) 1259
TaaI ACNGT 4 cut(s) 150, 664, 921, 967
TaqI TCGA 2 cut(s) 113, 219
TasI AATT 9 cut(s) 88, 554, 724, 847, 1145, 1435, 1459, 1467, 1581
TatI WGTACW 2 cut(s) 150, 300
TauI GCSGC 4 cut(s) 59, 62, 229, 1307
TfiI GAWTC 2 cut(s) 5, 387
Tru1I TTAA 7 cut(s) 24, 477, 525, 714, 1125, 1542, 1584
Tru9I TTAA 7 cut(s) 24, 477, 525, 714, 1125, 1542, 1584
TscAI CASTG 1 cut(s) 935
TseFI GTSAC 2 cut(s) 118, 1028
TseI GCWGC 3 cut(s) 82, 140, 730
Tsp45I GTSAC 2 cut(s) 118, 1028
TspDTI ATGAA 8 cut(s) 194, 278, 440, 651, 674, 767, 1257, 1300
TspGWI ACGGA 4 cut(s) 311, 1392, 1442, 1602
TspRI CASTG 1 cut(s) 935
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 1400
XapI RAATTY 6 cut(s) 88, 554, 847, 1145, 1435, 1459
XceI RCATGY 4 cut(s) 307, 476, 1113, 1607
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 1 cut(s) 1659
Zsp2I ATGCAT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.