pycom10g15710

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001303
Physical Location & Seq
Reverse (-)
18909463 .. 18911984
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15710.3

Sequence Viewer

Length: 1185 bp
ATGGATTCGGCAAGGAGTTGGCTGCAGAAGCTTCAGCCGCGCGACAAGATGAGGAACTCGACGAGGAGGAAGGAGCCGTGCGCCGGCGGCGATGGAGGCGGAGACGAGTCGAATATGGACGAGGAGGCACTTTCCAATGTTACGAAGCAGAAGGCTGCGGCGGCGAAGCAGTATATTGAGAACCATTACAAGGAGCAGATGAAGAACCTCCTGGAGCGAAAGGAACGACGAAATATGTTAGAGAAAAAGTTGGCTGATGCCGACGTCTCTGAGGAAGATCAAAGCAACCTTCTGAAGTTCTTGGAGAAGAAAGAAACCGAATACATGCGCCTTCAGAGGCATAAAATGGGTGTTGATGATTTTGAGTTGTTGACTATGATCGGCAAGGGAGCATTTGGTGAGGTTCGTGTATGCAGGGAAAAGACGACTGGTTCTGTATATGCCATGAAAAAGCTAAAGAAATCAGAGATGCTTCGTAGAGGCCAGGTAGAGCATGTCAAGGCTGAAAGGAACTTACTTGCTGAGGTGGACAGCAATTGCATTGTCAAACTGTACTGTTCGTTTCAAGATGATGACTATCTTTATCTTATAATGGAATACTTACCGGGAGGAGATATGATGACTTTGCTTATGAGAAAGGATATTTTGACTGATGATGAAGCTAGATTCTATGTTGCCGAAACAGTTTTGGCTATTGAATCTATCCATAAACACAATTACATTCATAGGGATATTAAGCCTGACAATTTGCTACTCGATAGATATGGACACTTAAGACTGTCAGATTTTGGGTTGTGTAAACCTTTAGACTGCAGTACTATCCGAGAAGATTTTTCAGTAGCACCCAATGCTAATGGCGCTGCACAGAATGATGAACGTCCTGCAGCTCCAAAACGAACACAACAAGAGCAACTGCAACATTGGCAGAAGAATAGGAGAATGCTTGCTTATTCGACTGTTGGTACCCCTGACTATATTGCTCCAGAAGTTCTGTTGAAAAAAGGTTATGGGATGGAATGCGATTGGTGGTCACTTGGTGCTATTATGTATGAAATGCTTGTGGGTTACCCACCCTTCTATTCTGATCGATCCAATGTCAACATGCAGGAAGATTGTAAACTGGAAAACTCATTTGAAATTTCCAGAAGAAGCAATGCTATCTCCAGGGGCAAAAGATCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

395

Amino Acids

45.66

Weight (kDa)

7.08

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
AatII GACGTC 1 cut(s) 267
Acc65I GGTACC 1 cut(s) 962
AccB1I GGYRCC 1 cut(s) 962
AccII CGCG 2 cut(s) 40, 42
AciI CCGC 5 cut(s) 38, 87, 99, 158, 161
AclWI GGATC 1 cut(s) 1083
AcsI RAATTY 1 cut(s) 1137
AcuI CTGAAG 3 cut(s) 17, 314, 317
AcyI GRCGYC 1 cut(s) 264
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 3 cut(s) 554, 817, 964
AfiI CCNNNNNNNGG 2 cut(s) 83, 190
AflII CTTAAG 1 cut(s) 772
AgsI TTSAA 4 cut(s) 566, 698, 997, 1136
AjnI CCWGG 3 cut(s) 210, 483, 1163
AluBI AGCT 4 cut(s) 31, 454, 662, 887
AluI AGCT 4 cut(s) 31, 454, 662, 887
Alw26I GTCTC 2 cut(s) 96, 271
AlwI GGATC 1 cut(s) 1083
AoxI GGCC 1 cut(s) 481
ApeKI GCWGC 4 cut(s) 22, 155, 860, 884
ApoI RAATTY 1 cut(s) 1137
Asp718I GGTACC 1 cut(s) 962
AspLEI GCGC 4 cut(s) 42, 83, 330, 860
AsuC2I CCSGG 1 cut(s) 606
AsuHPI GGTGA 1 cut(s) 410
BanI GGYRCC 1 cut(s) 962
BbvCI CCTCAGC 1 cut(s) 522
BbvI GCAGC 4 cut(s) 9, 142, 847, 896
BccI CCATC 2 cut(s) 86, 1006
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 3 cut(s) 212, 485, 1165
BcnI CCSGG 1 cut(s) 606
BcoDI GTCTC 2 cut(s) 96, 271
BfaI CTAG 1 cut(s) 663
BfmI CTRYAG 3 cut(s) 23, 811, 882
BfoI RGCGCY 1 cut(s) 861
BfrI CTTAAG 1 cut(s) 772
BglII AGATCT 1 cut(s) 1175
BisI GCNGC 8 cut(s) 23, 38, 88, 156, 159, 162, 861, 885
BlsI GCNGC 8 cut(s) 24, 39, 89, 157, 160, 163, 862, 886
BmcAI AGTACT 1 cut(s) 817
Bme1390I CCNGG 4 cut(s) 212, 485, 606, 1165
BmiI GGNNCC 2 cut(s) 75, 964
BmrFI CCNGG 4 cut(s) 212, 485, 606, 1165
BmsI GCATC 2 cut(s) 247, 459
BpmI CTGGAG 3 cut(s) 233, 966, 1147
Bpu10I CCTNAGC 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 606
Bsa29I ATCGAT 1 cut(s) 1087
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 1164
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 190
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 433, 1125
Bse3DI GCAATG 1 cut(s) 1159
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 3 cut(s) 212, 485, 1165
BseCI ATCGAT 1 cut(s) 1087
BseDI CCNNGG 1 cut(s) 1164
BseGI GGATG 1 cut(s) 1017
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 83, 190
BseMI GCAATG 1 cut(s) 1159
BseMII CTCAG 2 cut(s) 261, 513
BseNI ACTGG 2 cut(s) 433, 1125
BseRI GAGGAG 3 cut(s) 79, 137, 624
BseXI GCAGC 4 cut(s) 9, 142, 847, 896
BsgI GTGCAG 1 cut(s) 846
Bsh1236I CGCG 2 cut(s) 40, 42
BshFI GGCC 1 cut(s) 483
BshNI GGYRCC 1 cut(s) 962
BshVI ATCGAT 1 cut(s) 1087
BsiSI CCGG 2 cut(s) 84, 605
BslI CCNNNNNNNGG 2 cut(s) 83, 190
BsmAI GTCTC 2 cut(s) 96, 271
BsmBI CGTCTC 2 cut(s) 96, 271
BsmI GAATGC 2 cut(s) 945, 1022
BsnI GGCC 1 cut(s) 483
Bsp143I GATC 5 cut(s) 277, 378, 1084, 1088, 1175
BspACI CCGC 5 cut(s) 38, 87, 99, 158, 161
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 2 cut(s) 262, 514
BspDI ATCGAT 1 cut(s) 1087
BspFNI CGCG 2 cut(s) 40, 42
BspLI GGNNCC 2 cut(s) 75, 964
BspMAI CTGCAG 3 cut(s) 27, 815, 886
BspPI GGATC 1 cut(s) 1083
BspT107I GGYRCC 1 cut(s) 962
BspTI CTTAAG 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 1159
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 433, 1125
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 1164
BssMI GATC 5 cut(s) 277, 378, 1084, 1088, 1175
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 212, 485, 1165
Bst4CI ACNGT 5 cut(s) 552, 557, 685, 780, 958
BstACI GRCGYC 1 cut(s) 264
BstAFI CTTAAG 1 cut(s) 772
BstAPI GCANNNNNTGC 1 cut(s) 848
BstC8I GCNNGC 2 cut(s) 85, 945
BstDEI CTNAG 2 cut(s) 270, 522
BstEII GGTNACC 1 cut(s) 1064
BstF5I GGATG 1 cut(s) 1017
BstFNI CGCG 2 cut(s) 40, 42
BstH2I RGCGCY 1 cut(s) 861
BstHHI GCGC 4 cut(s) 42, 83, 330, 860
BstKTI GATC 5 cut(s) 280, 381, 1087, 1091, 1178
BstMAI GTCTC 2 cut(s) 96, 271
BstMBI GATC 5 cut(s) 277, 378, 1084, 1088, 1175
BstMWI GCNNNNNNNGC 8 cut(s) 28, 37, 87, 96, 161, 848, 857, 922
BstNI CCWGG 3 cut(s) 212, 485, 1165
BstNSI RCATGY 3 cut(s) 328, 497, 1105
BstPI GGTNACC 1 cut(s) 1064
BstSCI CCNGG 4 cut(s) 210, 483, 604, 1163
BstSFI CTRYAG 3 cut(s) 23, 811, 882
BstUI CGCG 2 cut(s) 40, 42
BstV1I GCAGC 4 cut(s) 9, 142, 847, 896
BstX2I RGATCY 1 cut(s) 1175
BstYI RGATCY 1 cut(s) 1175
Bsu15I ATCGAT 1 cut(s) 1087
BsuRI GGCC 1 cut(s) 483
BsuTUI ATCGAT 1 cut(s) 1087
BtgZI GCGATG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 1017
Cac8I GCNNGC 2 cut(s) 85, 945
CfoI GCGC 4 cut(s) 42, 83, 330, 860
Cfr10I RCCGGY 1 cut(s) 83
ClaI ATCGAT 1 cut(s) 1087
Csp6I GTAC 3 cut(s) 553, 816, 963
CviAII CATG 4 cut(s) 325, 445, 494, 1102
CviQI GTAC 3 cut(s) 553, 816, 963
DdeI CTNAG 2 cut(s) 270, 522
DpnI GATC 5 cut(s) 279, 380, 1086, 1090, 1177
DpnII GATC 5 cut(s) 277, 378, 1084, 1088, 1175
DraIII CACNNNGTG 1 cut(s) 1037
EciI GGCGGA 1 cut(s) 114
Eco57I CTGAAG 3 cut(s) 17, 314, 317
Eco91I GGTNACC 1 cut(s) 1064
EcoO65I GGTNACC 1 cut(s) 1064
EcoRII CCWGG 3 cut(s) 210, 483, 1163
Esp3I CGTCTC 2 cut(s) 96, 271
FaeI CATG 4 cut(s) 328, 448, 497, 1105
FatI CATG 4 cut(s) 324, 444, 493, 1101
Fnu4HI GCNGC 8 cut(s) 23, 38, 88, 156, 159, 162, 861, 885
FokI GGATG 1 cut(s) 1024
Fsp4HI GCNGC 8 cut(s) 23, 38, 88, 156, 159, 162, 861, 885
FspBI CTAG 1 cut(s) 663
GlaI GCGC 4 cut(s) 41, 82, 329, 859
GluI GCNGC 8 cut(s) 23, 38, 88, 156, 159, 162, 861, 885
GsuI CTGGAG 3 cut(s) 233, 966, 1147
HaeII RGCGCY 1 cut(s) 861
HaeIII GGCC 1 cut(s) 483
HapII CCGG 2 cut(s) 84, 605
HhaI GCGC 4 cut(s) 42, 83, 330, 860
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 4 cut(s) 328, 448, 497, 1105
Hin6I GCGC 4 cut(s) 40, 81, 328, 858
HinP1I GCGC 4 cut(s) 40, 81, 328, 858
HincII GTYRAC 2 cut(s) 372, 1099
HindII GTYRAC 2 cut(s) 372, 1099
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 4 cut(s) 5, 107, 666, 698
HpaII CCGG 2 cut(s) 84, 605
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 5 cut(s) 372, 529, 800, 1099, 1118
Hpy188I TCNGA 7 cut(s) 271, 294, 336, 466, 784, 824, 1084
Hpy188III TCNNGA 3 cut(s) 566, 983, 1143
Hpy8I GTNNAC 5 cut(s) 372, 529, 800, 1099, 1118
Hpy99I CGWCG 3 cut(s) 64, 231, 266
HpyAV CCTTC 5 cut(s) 64, 145, 299, 341, 1084
HpyCH4III ACNGT 5 cut(s) 552, 557, 685, 780, 958
HpyCH4IV ACGT 2 cut(s) 264, 877
HpyCH4V TGCA 8 cut(s) 25, 414, 540, 813, 863, 884, 916, 1105
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 37, 87, 96, 161, 848, 857, 922
HpyF3I CTNAG 2 cut(s) 270, 522
HpySE526I ACGT 2 cut(s) 264, 877
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 4 cut(s) 328, 448, 497, 1105
HspAI GCGC 4 cut(s) 40, 81, 328, 858
KpnI GGTACC 1 cut(s) 966
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Kzo9I GATC 5 cut(s) 277, 378, 1084, 1088, 1175
LmnI GCTCC 6 cut(s) 73, 193, 214, 389, 892, 985
Lsp1109I GCAGC 4 cut(s) 9, 142, 847, 896
LweI GCATC 2 cut(s) 247, 459
MaeI CTAG 1 cut(s) 663
MaeII ACGT 2 cut(s) 264, 877
MaeIII GTNAC 3 cut(s) 139, 1029, 1064
MalI GATC 5 cut(s) 279, 380, 1086, 1090, 1177
MboI GATC 5 cut(s) 277, 378, 1084, 1088, 1175
MboII GAAGA 7 cut(s) 214, 287, 319, 839, 940, 1121, 1158
MfeI CAATTG 1 cut(s) 535
MflI RGATCY 1 cut(s) 1175
MluCI AATT 4 cut(s) 535, 715, 745, 1137
MlyI GAGTC 1 cut(s) 116
MreI CGCCGGCG 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 2 cut(s) 735, 773
MspCI CTTAAG 1 cut(s) 772
MspI CCGG 2 cut(s) 84, 605
MspR9I CCNGG 4 cut(s) 212, 485, 606, 1165
MunI CAATTG 1 cut(s) 535
Mva1269I GAATGC 2 cut(s) 945, 1022
MvaI CCWGG 3 cut(s) 212, 485, 1165
MvnI CGCG 2 cut(s) 40, 42
MwoI GCNNNNNNNGC 8 cut(s) 28, 37, 87, 96, 161, 848, 857, 922
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 606
NdeII GATC 5 cut(s) 277, 378, 1084, 1088, 1175
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 4 cut(s) 328, 448, 497, 1105
NlaIV GGNNCC 2 cut(s) 75, 964
NmuCI GTSAC 1 cut(s) 1029
NspI RCATGY 3 cut(s) 328, 497, 1105
PctI GAATGC 2 cut(s) 945, 1022
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 5, 666, 698
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 8 cut(s) 24, 39, 89, 157, 160, 163, 862, 886
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PsiI TTATAA 1 cut(s) 590
Psp6I CCWGG 3 cut(s) 210, 483, 1163
PspEI GGTNACC 1 cut(s) 1064
PspGI CCWGG 3 cut(s) 210, 483, 1163
PspN4I GGNNCC 2 cut(s) 75, 964
PstI CTGCAG 3 cut(s) 27, 815, 886
PsuI RGATCY 1 cut(s) 1175
RsaI GTAC 3 cut(s) 554, 817, 964
RsaNI GTAC 3 cut(s) 553, 816, 963
SaqAI TTAA 2 cut(s) 735, 773
SatI GCNGC 8 cut(s) 23, 38, 88, 156, 159, 162, 861, 885
Sau3AI GATC 5 cut(s) 277, 378, 1084, 1088, 1175
ScaI AGTACT 1 cut(s) 817
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 212, 485, 606, 1165
SfaNI GCATC 2 cut(s) 247, 459
SfcI CTRYAG 3 cut(s) 23, 811, 882
SgrAI CRCCGGYG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 4 cut(s) 535, 715, 745, 1137
SsiI CCGC 5 cut(s) 38, 87, 99, 158, 161
SspMI CTAG 1 cut(s) 663
StyD4I CCNGG 4 cut(s) 210, 483, 604, 1163
TaaI ACNGT 5 cut(s) 552, 557, 685, 780, 958
TaiI ACGT 2 cut(s) 267, 880
TaqI TCGA 5 cut(s) 59, 110, 756, 953, 1087
TasI AATT 4 cut(s) 535, 715, 745, 1137
TatI WGTACW 2 cut(s) 552, 815
TauI GCSGC 4 cut(s) 40, 90, 161, 164
TfiI GAWTC 3 cut(s) 5, 666, 698
Tru1I TTAA 2 cut(s) 735, 773
Tru9I TTAA 2 cut(s) 735, 773
TseFI GTSAC 1 cut(s) 1029
TseI GCWGC 4 cut(s) 22, 155, 860, 884
Tsp45I GTSAC 1 cut(s) 1029
TspDTI ATGAA 6 cut(s) 215, 461, 672, 713, 888, 1065
Vha464I CTTAAG 1 cut(s) 772
XapI RAATTY 1 cut(s) 1137
XceI RCATGY 3 cut(s) 328, 497, 1105
XspI CTAG 1 cut(s) 663
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.