FvH4_2g21180

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001303
Physical Location & Seq
Reverse (-)
17626077 .. 17631503
5427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21180.t6

Sequence Viewer

Length: 1659 bp
ATGGATTCGGCAAGGCAATGGTTGCAGAAGCTACAGCCGCGAGATAAAGTGAGGAGCTACGCGAGGAAGAAAGACACGGTGGGCGGCGAAGACGGCTCGGGTACCGATGACGAAGCGCTTTCTAATGTCACCAAGCAGAAGGCCGCCGCGGCCAAGCAGTACATTGAGAACCATTACAAAGAGCAGATGAAGAATCTGCAGGAGAGGAGAGAAAGACGGAATATGCTGGAAAAGAAGCTGGCTGATGCAGATGTGTCGGAGGAGGATCAGAACAACCTTCTTAAGTTCTTGGAGAAGAAAGAGACCGAGTACATGCGGCTGCAGAGGCATAAAATGGGGTGTGATGATTTTGAGTTGTTGACCATGATCGGCAAGGGCGCATTTGGTGAGGTTCGTGTCTGCAGGGAAAAGACATCAGGTTCTGTATATGCCATGAAAAAGCTTAAGAAGTCAGAAATGCTTCGTAGAGGCCAGGTTGAGCATGTAAAAGCTGAAAGGAATCTGCTTGCTGAGGTGGACAGCAATTGCATCGTCAAGCTCTATTGTTCTTTTCAAGATGATGACTATCTTTATCTTATAATGGAATACTTACCTGGAGGAGATATGATGACTTTACTTATGAGAAAGGATATTTTGACTGAAGATGAGGCTAGATTTTATGTCGCTGAAACAGTTTTGGCTATTGAGTCTATCCATAAACACAATTATATTCATAGGGATATTAAGCCTGATAATTTACTACTTGATAGATATGGACACTTGAGACTGTCAGATTTCGGATTATGCAAGCCTTTAGATTGCAGTACAATTCATGAAGACTTTTCAGTAGCAAATAATACCAATGGTTCTGGACATAATGATGAACGCCCTGCAGCTCCAAAACGCACACAACAAGAGCAACTGCAACATTGGCAGAAGAATAGGAGAATGCTTGCTTATTCAACTGTTGGTACACCTGATTATATTGCCCCAGAAGTTCTGTTGAAAAAAGGATATGGAATGGAATGTGATTGGTGGTCACTTGGTGCTATTATGTATGAAATGCTTGTCGGTTATCCGCCCTTCTATTCTGATGATCCAATGTCAACCTGCAGGAAGATAGTAAACTGGAAAACTCATTTGAAATTTCCAGAAGAAGCAAAGCTATCTCCAGAGGCAAAAGATCTTATTAGTAAACTCTTGTGTAATGTCAACCAAAGGTTGGGAACAAATGGTGCAAGTGAAATTAAGGTTCATCCTTGGTTTGATGGTACTGAATGGGAAAAGCTTTACCAAATGGATGCTGCATTTATTCCTGAGGTCAATGATGATCTGGACACTCAAAATTTTGAAAAGTTTGAAGAGGCTGACAACCAAGCTCAGACTTCACAAAAAACAGGTCCCTGGAGAAAGATGCTCTCCTCCAAGGATATCAATTTCGTGGGCTACACATATAAAAACTTTGAAATTGTAAACGATTATCAAGTACCAGGCATGGCCTCGTTGAAGAAGAAGACCAACACATCAAAACGGCCATCCATAAAAACACTTTTTGATAATGAGTCAGAGTCATCGGAGGCATCCGACACACCTGGTAGTGAACAACCTGTGAAAGGCAGCTTTTTGAACTTGTTGCCTCCCGAATTAGAAGTACCTCAACGCCGAAGTGACTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

553

Amino Acids

63.63

Weight (kDa)

5.96

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 118 - 268 4.9e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 118 - 265 2.7e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 312 - 415 1.4e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 578
Acc36I ACCTGC 1 cut(s) 1097
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 1201
AccII CGCG 3 cut(s) 40, 62, 149
AciI CCGC 7 cut(s) 38, 84, 144, 147, 149, 316, 1058
AclWI GGATC 2 cut(s) 273, 1070
AcoI YGGCCR 2 cut(s) 150, 1511
AcsI RAATTY 2 cut(s) 1124, 1324
AcuI CTGAAG 1 cut(s) 660
AdeI CACNNNGTG 1 cut(s) 1025
AfaI GTAC 8 cut(s) 103, 161, 311, 805, 952, 1252, 1467, 1632
AfeI AGCGCT 1 cut(s) 117
AfiI CCNNNNNNNGG 2 cut(s) 1201, 1592
AflII CTTAAG 2 cut(s) 281, 443
AgsI TTSAA 9 cut(s) 554, 942, 985, 1123, 1331, 1340, 1445, 1486, 1606
AjnI CCWGG 5 cut(s) 471, 592, 1382, 1468, 1570
Alw26I GTCTC 2 cut(s) 296, 757
AlwI GGATC 2 cut(s) 273, 1070
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 97
Aor51HI AGCGCT 1 cut(s) 117
AoxI GGCC 5 cut(s) 141, 150, 469, 1476, 1511
ApeKI GCWGC 4 cut(s) 319, 872, 1283, 1596
ApoI RAATTY 2 cut(s) 1124, 1324
ArsI GACNNNNNNTTYG 2 cut(s) 1363, 1395
Asp700I GAANNNNTTC 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 118, 380
AspS9I GGNCC 1 cut(s) 1379
AsuHPI GGTGA 2 cut(s) 121, 398
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 1 cut(s) 1379
AxyI CCTNAGG 1 cut(s) 1296
BanI GGYRCC 1 cut(s) 101
BbsI GAAGAC 3 cut(s) 96, 822, 1499
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 4 cut(s) 306, 884, 1270, 1608
BccI CCATC 2 cut(s) 1241, 1522
BceAI ACGGC 2 cut(s) 109, 1526
BcgI CGANNNNNNTGC 4 cut(s) 237, 271, 511, 545
BciT130I CCWGG 5 cut(s) 473, 594, 1384, 1470, 1572
BcoDI GTCTC 2 cut(s) 296, 757
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 6 cut(s) 32, 197, 320, 400, 870, 1090
BfoI RGCGCY 1 cut(s) 119
BfrI CTTAAG 2 cut(s) 281, 443
BfuAI ACCTGC 1 cut(s) 1097
BglI GCCNNNNNGGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 1162
Bme1390I CCNGG 5 cut(s) 473, 594, 1384, 1470, 1572
Bme18I GGWCC 1 cut(s) 1379
BmeT110I CYCGRG 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 1379
BmiI GGNNCC 2 cut(s) 103, 1381
BmrFI CCNGG 5 cut(s) 473, 594, 1384, 1470, 1572
BmsI GCATC 5 cut(s) 235, 537, 1270, 1383, 1568
BpiI GAAGAC 3 cut(s) 96, 822, 1499
BpmI CTGGAG 3 cut(s) 615, 1134, 1405
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 781
BsaBI GATNNNNATC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 147, 1238, 1382, 1404
Bsc4I CCNNNNNNNGG 2 cut(s) 1201, 1592
Bse1I ACTGG 1 cut(s) 1112
Bse21I CCTNAGG 1 cut(s) 1296
Bse3DI GCAATG 1 cut(s) 23
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 5 cut(s) 473, 594, 1384, 1470, 1572
BseDI CCNNGG 4 cut(s) 147, 1238, 1382, 1404
BseGI GGATG 4 cut(s) 1234, 1285, 1514, 1559
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 2 cut(s) 1201, 1592
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 3 cut(s) 501, 1287, 1373
BseNI ACTGG 1 cut(s) 1112
BseRI GAGGAG 5 cut(s) 67, 220, 275, 612, 1390
BseXI GCAGC 4 cut(s) 306, 884, 1270, 1608
Bsh1236I CGCG 3 cut(s) 40, 62, 149
BshFI GGCC 5 cut(s) 143, 152, 471, 1478, 1513
BshNI GGYRCC 1 cut(s) 101
BsiHKCI CYCGRG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 1365
BslI CCNNNNNNNGG 2 cut(s) 1201, 1592
BsmAI GTCTC 2 cut(s) 296, 757
BsmFI GGGAC 1 cut(s) 1365
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 5 cut(s) 143, 152, 471, 1478, 1513
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 97
Bsp143I GATC 5 cut(s) 265, 366, 1075, 1162, 1309
BspACI CCGC 7 cut(s) 38, 84, 144, 147, 149, 316, 1058
BspANI GGCC 5 cut(s) 143, 152, 471, 1478, 1513
BspCNI CTCAG 3 cut(s) 502, 1288, 1372
BspFNI CGCG 3 cut(s) 40, 62, 149
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 2 cut(s) 103, 1381
BspMAI CTGCAG 5 cut(s) 201, 324, 404, 874, 1094
BspMI ACCTGC 1 cut(s) 1097
BspPI GGATC 2 cut(s) 273, 1070
BspT107I GGYRCC 1 cut(s) 101
BspTI CTTAAG 2 cut(s) 281, 443
BspTNI GGTCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 23
BsrI ACTGG 1 cut(s) 1112
BssECI CCNNGG 4 cut(s) 147, 1238, 1382, 1404
BssMI GATC 5 cut(s) 265, 366, 1075, 1162, 1309
BssT1I CCWWGG 2 cut(s) 1238, 1404
Bst2UI CCWGG 5 cut(s) 473, 594, 1384, 1470, 1572
Bst4CI ACNGT 4 cut(s) 79, 673, 768, 946
Bst6I CTCTTC 1 cut(s) 1335
BstAFI CTTAAG 2 cut(s) 281, 443
BstAPI GCANNNNNTGC 1 cut(s) 22
BstC8I GCNNGC 4 cut(s) 240, 507, 788, 933
BstDEI CTNAG 3 cut(s) 510, 1296, 1359
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 1590
BstF5I GGATG 4 cut(s) 1234, 1285, 1514, 1559
BstFNI CGCG 3 cut(s) 40, 62, 149
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 2 cut(s) 118, 380
BstKTI GATC 5 cut(s) 268, 369, 1078, 1165, 1312
BstMAI GTCTC 2 cut(s) 296, 757
BstMBI GATC 5 cut(s) 265, 366, 1075, 1162, 1309
BstMWI GCNNNNNNNGC 6 cut(s) 22, 37, 93, 149, 325, 910
BstNI CCWGG 5 cut(s) 473, 594, 1384, 1470, 1572
BstNSI RCATGY 2 cut(s) 316, 485
BstSCI CCNGG 5 cut(s) 471, 592, 1382, 1468, 1570
BstSFI CTRYAG 6 cut(s) 32, 197, 320, 400, 870, 1090
BstUI CGCG 3 cut(s) 40, 62, 149
BstV1I GCAGC 4 cut(s) 306, 884, 1270, 1608
BstV2I GAAGAC 3 cut(s) 96, 822, 1499
BstX2I RGATCY 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1162
Bsu36I CCTNAGG 1 cut(s) 1296
BsuRI GGCC 5 cut(s) 143, 152, 471, 1478, 1513
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 4 cut(s) 1234, 1285, 1514, 1559
BveI ACCTGC 1 cut(s) 1097
Cac8I GCNNGC 4 cut(s) 240, 507, 788, 933
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 1 cut(s) 811
CfoI GCGC 2 cut(s) 118, 380
Cfr13I GGNCC 1 cut(s) 1379
Cfr42I CCGCGG 1 cut(s) 150
CsiI ACCWGGT 1 cut(s) 1570
Csp6I GTAC 8 cut(s) 102, 160, 310, 804, 951, 1251, 1466, 1631
CviAII CATG 6 cut(s) 313, 364, 433, 482, 812, 1474
CviQI GTAC 8 cut(s) 102, 160, 310, 804, 951, 1251, 1466, 1631
DdeI CTNAG 3 cut(s) 510, 1296, 1359
DpnI GATC 5 cut(s) 267, 368, 1077, 1164, 1311
DpnII GATC 5 cut(s) 265, 366, 1075, 1162, 1309
DraIII CACNNNGTG 1 cut(s) 1025
EaeI YGGCCR 2 cut(s) 150, 1511
Eam1104I CTCTTC 1 cut(s) 1335
EarI CTCTTC 1 cut(s) 1335
EciI GGCGGA 1 cut(s) 1047
Eco130I CCWWGG 2 cut(s) 1238, 1404
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 1411
Eco47I GGWCC 1 cut(s) 1379
Eco47III AGCGCT 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 660
Eco81I CCTNAGG 1 cut(s) 1296
Eco88I CYCGRG 1 cut(s) 97
EcoNI CCTNNNNNAGG 1 cut(s) 1590
EcoO109I RGGNCCY 1 cut(s) 1379
EcoRII CCWGG 5 cut(s) 471, 592, 1382, 1468, 1570
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 2 cut(s) 1238, 1404
ErhI CCWWGG 2 cut(s) 1238, 1404
FaeI CATG 6 cut(s) 316, 367, 436, 485, 815, 1477
FalI AAGNNNNNCTT 1 cut(s) 1636
FaqI GGGAC 1 cut(s) 1365
FatI CATG 6 cut(s) 312, 363, 432, 481, 811, 1473
FokI GGATG 4 cut(s) 1221, 1292, 1501, 1546
FspBI CTAG 1 cut(s) 651
GlaI GCGC 2 cut(s) 117, 379
GsuI CTGGAG 3 cut(s) 615, 1134, 1405
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 5 cut(s) 143, 152, 471, 1478, 1513
HhaI GCGC 2 cut(s) 118, 380
Hin1II CATG 6 cut(s) 316, 367, 436, 485, 815, 1477
Hin6I GCGC 2 cut(s) 116, 378
HinP1I GCGC 2 cut(s) 116, 378
HincII GTYRAC 3 cut(s) 360, 1086, 1192
HindII GTYRAC 3 cut(s) 360, 1086, 1192
HindIII AAGCTT 2 cut(s) 440, 1265
HinfI GANTC 7 cut(s) 5, 193, 499, 686, 1541, 1547, 1649
HphI GGTGA 2 cut(s) 121, 398
Hpy166II GTNNAC 9 cut(s) 360, 517, 953, 1086, 1105, 1175, 1192, 1453, 1580
Hpy188III TCNNGA 8 cut(s) 554, 812, 849, 1130, 1151, 1295, 1313, 1619
Hpy8I GTNNAC 9 cut(s) 360, 517, 953, 1086, 1105, 1175, 1192, 1453, 1580
HpyAV CCTTC 3 cut(s) 133, 287, 1072
HpyCH4III ACNGT 4 cut(s) 79, 673, 768, 946
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 37, 93, 149, 325, 910
HpyF3I CTNAG 3 cut(s) 510, 1296, 1359
Hsp92II CATG 6 cut(s) 316, 367, 436, 485, 815, 1477
HspAI GCGC 2 cut(s) 116, 378
KpnI GGTACC 1 cut(s) 105
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 5 cut(s) 265, 366, 1075, 1162, 1309
LmnI GCTCC 2 cut(s) 54, 880
Lsp1109I GCAGC 4 cut(s) 306, 884, 1270, 1608
LweI GCATC 5 cut(s) 235, 537, 1270, 1383, 1568
MabI ACCWGGT 1 cut(s) 1570
MaeI CTAG 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 127, 1017, 1646
MalI GATC 5 cut(s) 267, 368, 1077, 1164, 1311
MboI GATC 5 cut(s) 265, 366, 1075, 1162, 1309
MfeI CAATTG 1 cut(s) 523
MflI RGATCY 1 cut(s) 1162
MlyI GAGTC 4 cut(s) 695, 1550, 1556, 1643
MmeI TCCRAC 2 cut(s) 237, 1587
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 4 cut(s) 282, 444, 723, 1227
MslI CAYNNNNRTG 1 cut(s) 858
MspA1I CMGCKG 1 cut(s) 149
MspCI CTTAAG 2 cut(s) 281, 443
MspR9I CCNGG 5 cut(s) 473, 594, 1384, 1470, 1572
MunI CAATTG 1 cut(s) 523
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 5 cut(s) 473, 594, 1384, 1470, 1572
MvnI CGCG 3 cut(s) 40, 62, 149
MwoI GCNNNNNNNGC 6 cut(s) 22, 37, 93, 149, 325, 910
NdeII GATC 5 cut(s) 265, 366, 1075, 1162, 1309
NlaIII CATG 6 cut(s) 316, 367, 436, 485, 815, 1477
NlaIV GGNNCC 2 cut(s) 103, 1381
NmuCI GTSAC 3 cut(s) 127, 1017, 1646
NspI RCATGY 2 cut(s) 316, 485
PagI TCATGA 1 cut(s) 811
PctI GAATGC 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 3 cut(s) 5, 193, 499
PflMI CCANNNNNTGG 1 cut(s) 1201
PleI GAGTC 4 cut(s) 694, 1549, 1555, 1643
PpsI GAGTC 4 cut(s) 694, 1549, 1555, 1643
PpuMI RGGWCCY 1 cut(s) 1379
PsiI TTATAA 1 cut(s) 578
Psp5II RGGWCCY 1 cut(s) 1379
Psp6I CCWGG 5 cut(s) 471, 592, 1382, 1468, 1570
PspGI CCWGG 5 cut(s) 471, 592, 1382, 1468, 1570
PspN4I GGNNCC 2 cut(s) 103, 1381
PspPI GGNCC 1 cut(s) 1379
PspPPI RGGWCCY 1 cut(s) 1379
PstI CTGCAG 5 cut(s) 201, 324, 404, 874, 1094
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 1162
RsaI GTAC 8 cut(s) 103, 161, 311, 805, 952, 1252, 1467, 1632
RsaNI GTAC 8 cut(s) 102, 160, 310, 804, 951, 1251, 1466, 1631
RseI CAYNNNNRTG 1 cut(s) 858
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 4 cut(s) 282, 444, 723, 1227
Sau3AI GATC 5 cut(s) 265, 366, 1075, 1162, 1309
Sau96I GGNCC 1 cut(s) 1379
SbfI CCTGCAGG 1 cut(s) 1094
SchI GAGTC 4 cut(s) 695, 1550, 1556, 1643
ScrFI CCNGG 5 cut(s) 473, 594, 1384, 1470, 1572
SdaI CCTGCAGG 1 cut(s) 1094
SexAI ACCWGGT 1 cut(s) 1570
SfaNI GCATC 5 cut(s) 235, 537, 1270, 1383, 1568
SfcI CTRYAG 6 cut(s) 32, 197, 320, 400, 870, 1090
SfiI GGCCNNNNNGGCC 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 1 cut(s) 1379
SmiMI CAYNNNNRTG 1 cut(s) 858
SmlI CTYRAG 3 cut(s) 281, 443, 760
SmoI CTYRAG 3 cut(s) 281, 443, 760
Sse8387I CCTGCAGG 1 cut(s) 1094
SsiI CCGC 7 cut(s) 38, 84, 144, 147, 149, 316, 1058
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 5 cut(s) 471, 592, 1382, 1468, 1570
StyI CCWWGG 2 cut(s) 1238, 1404
TaaI ACNGT 4 cut(s) 79, 673, 768, 946
TaqII GACCGA 1 cut(s) 320
TatI WGTACW 3 cut(s) 159, 309, 803
TauI GCSGC 6 cut(s) 40, 87, 146, 149, 152, 319
TfiI GAWTC 3 cut(s) 5, 193, 499
Tru1I TTAA 4 cut(s) 282, 444, 723, 1227
Tru9I TTAA 4 cut(s) 282, 444, 723, 1227
TseFI GTSAC 3 cut(s) 127, 1017, 1646
TseI GCWGC 4 cut(s) 319, 872, 1283, 1596
Tsp45I GTSAC 3 cut(s) 127, 1017, 1646
TspDTI ATGAA 9 cut(s) 203, 449, 701, 800, 828, 876, 1053, 1223, 1644
TspGWI ACGGA 1 cut(s) 232
Van91I CCANNNNNTGG 1 cut(s) 1201
Vha464I CTTAAG 2 cut(s) 281, 443
VpaK11BI GGWCC 1 cut(s) 1379
XagI CCTNNNNNAGG 1 cut(s) 1590
XapI RAATTY 2 cut(s) 1124, 1324
XceI RCATGY 2 cut(s) 316, 485
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.