AT2G21385

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011302
Physical Location & Seq
Forward (+)
9149577 .. 9151970
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21385.3

Sequence Viewer

Length: 993 bp
ATGGCGGCAATATCCCCTGTTCAATGCTTATTCTCTTGCAATTCTCATCATCATCATCAACAACGCGAGCAGAGTGGGTCGCTTTGGAGATTGGGTTCTAGGGTTTTAGTATCTTCGAGTCTATGGAAGCTTTCTCTGTTCTCTGGACTCCATTTGAACCGAAGGAGAACTTTGATCTGTGCAGTCAAGGGAGATCCAGAAGATGCCTTTAAGAAGACTGTCGAAATCGATCGGATGATTGATGCTCTTAGAGATGCAAACCCTAGACAAGTTGAGAAGATTGTTGTTGAAAATATCCTTGCTTTTGATGAGGTTTTTTGGATAAGACTAGCAGCTAGGTCGGATACTTGTAAATCAGAAGATGACAAGAAGGACTATGAGGAGTTAGCTACAACTGTGATGACTATAGTTGACTGTGTTGTGAATAAGACTCGGGAAAAGATAGAGACGTCTACTGATATTCTGAAAGGGATTTTAAGACCTGCTATAGATGGAGTGGAAGAGATTTCATGGCCGCCAAGAGATCCTGAAGCCATCAATCTAATGAAAAAGGAAATTATACAACGGGAAAAAGAAGGGCAATTAGATGAAGGATTTCTTTCAGAAGTTAATGCTCAGCTCCGGCAGGCAAAAGAAGATAAAGACAAACCAGGGTTATTGGCTATGCTGCAAAAAGTTCTTCAACTCTATTCTGCTACTATCCTCTCCAAGCGTAGCTACGCAAAGAAAGGGAACGAAGTTGTAAAAGCCGAGCATTTTCTTGAAACTCTGATCAAAGCTCCTGAGGAACAATGGAACAAGCTTTTCGTAGACGGGTTAACAATCGGAAAAGGAGATATCACACCTGATGAGCTATCTGCTGTTATCAAGAAACGAATCGAGCGCACACTGATTCGAACTGAAGGGGGTTCATACCAGCAGCGAATTTTGACCGAGTATCTCAAAGGAATCGAGTCCAGGGCTAATGAAATTATGAAATTACTCCAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

37.55

Weight (kDa)

7.02

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 452
Acc36I ACCTGC 1 cut(s) 490
AccI GTMKAC 2 cut(s) 452, 810
AccII CGCG 1 cut(s) 66
AciI CCGC 2 cut(s) 5, 515
AclWI GGATC 2 cut(s) 188, 518
AcoI YGGCCR 1 cut(s) 512
AcsI RAATTY 1 cut(s) 924
AcuI CTGAAG 2 cut(s) 549, 921
AcyI GRCGYC 1 cut(s) 449
AgsI TTSAA 5 cut(s) 23, 157, 290, 683, 764
AjnI CCWGG 3 cut(s) 649, 956, 984
AloI GAACNNNNNNTCC 2 cut(s) 79, 111
AluBI AGCT 8 cut(s) 130, 335, 389, 619, 717, 779, 802, 853
AluI AGCT 8 cut(s) 130, 335, 389, 619, 717, 779, 802, 853
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 2 cut(s) 188, 518
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 512
ApeKI GCWGC 3 cut(s) 332, 667, 919
ApoI RAATTY 1 cut(s) 924
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 1 cut(s) 885
AsuII TTCGAA 1 cut(s) 895
AvaI CYCGRG 1 cut(s) 432
AxyI CCTNAGG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 221
BbvI GCAGC 3 cut(s) 344, 654, 931
BccI CCATC 2 cut(s) 485, 542
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BciT130I CCWGG 3 cut(s) 651, 958, 986
BciVI GTATCC 1 cut(s) 337
BclI TGATCA 1 cut(s) 771
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 4 cut(s) 99, 264, 329, 336
BfmI CTRYAG 2 cut(s) 405, 486
BfuAI ACCTGC 1 cut(s) 490
BfuI GTATCC 1 cut(s) 337
BisI GCNGC 5 cut(s) 6, 333, 515, 668, 920
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 5 cut(s) 7, 334, 516, 669, 921
Bme1390I CCNGG 3 cut(s) 651, 958, 986
BmeT110I CYCGRG 1 cut(s) 432
BmrFI CCNGG 3 cut(s) 651, 958, 986
BmsI GCATC 3 cut(s) 193, 232, 244
BpiI GAAGAC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 968
Bpu1102I GCTNAGC 1 cut(s) 615
Bpu14I TTCGAA 1 cut(s) 895
Bsa29I ATCGAT 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 449
BsaJI CCNNGG 3 cut(s) 650, 957, 985
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bse21I CCTNAGG 1 cut(s) 783
BseBI CCWGG 3 cut(s) 651, 958, 986
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 650, 957, 985
BseGI GGATG 1 cut(s) 240
BseMII CTCAG 2 cut(s) 629, 774
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 3 cut(s) 344, 654, 931
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 66
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 1 cut(s) 514
BshVI ATCGAT 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 232
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 1 cut(s) 622
BsmAI GTCTC 1 cut(s) 440
BsmBI CGTCTC 1 cut(s) 440
BsnI GGCC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 895
Bsp143I GATC 5 cut(s) 174, 193, 229, 523, 771
Bsp1720I GCTNAGC 1 cut(s) 615
BspACI CCGC 2 cut(s) 5, 515
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 2 cut(s) 628, 775
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 1 cut(s) 66
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 2 cut(s) 188, 518
BspT104I TTCGAA 1 cut(s) 895
BssECI CCNNGG 3 cut(s) 650, 957, 985
BssMI GATC 5 cut(s) 174, 193, 229, 523, 771
BssNI GRCGYC 1 cut(s) 449
Bst2UI CCWGG 3 cut(s) 651, 958, 986
Bst4CI ACNGT 3 cut(s) 220, 397, 416
Bst6I CTCTTC 1 cut(s) 495
BstACI GRCGYC 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 895
BstC8I GCNNGC 2 cut(s) 68, 627
BstDEI CTNAG 3 cut(s) 248, 615, 783
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 66
BstHHI GCGC 1 cut(s) 885
BstKTI GATC 5 cut(s) 177, 196, 232, 526, 774
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 5 cut(s) 174, 193, 229, 523, 771
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 3 cut(s) 651, 958, 986
BstSCI CCNGG 3 cut(s) 649, 956, 984
BstSFI CTRYAG 2 cut(s) 405, 486
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 344, 654, 931
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 2 cut(s) 193, 523
BstYI RGATCY 2 cut(s) 193, 523
Bsu15I ATCGAT 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 783
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 514
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 887
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 68, 627
CfoI GCGC 1 cut(s) 885
ClaI ATCGAT 1 cut(s) 228
CviAII CATG 1 cut(s) 510
DdeI CTNAG 3 cut(s) 248, 615, 783
DpnI GATC 5 cut(s) 176, 195, 231, 525, 773
DpnII GATC 5 cut(s) 174, 193, 229, 523, 771
EaeI YGGCCR 1 cut(s) 512
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Eco32I GATATC 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 549, 921
Eco81I CCTNAGG 1 cut(s) 783
Eco88I CYCGRG 1 cut(s) 432
EcoRII CCWGG 3 cut(s) 649, 956, 984
EcoRV GATATC 1 cut(s) 838
Esp3I CGTCTC 1 cut(s) 440
FaeI CATG 1 cut(s) 513
FaiI YATR 9 cut(s) 124, 378, 407, 488, 511, 560, 665, 913, 974
FalI AAGNNNNNCTT 4 cut(s) 154, 186, 582, 614
FatI CATG 1 cut(s) 509
FbaI TGATCA 1 cut(s) 771
FblI GTMKAC 2 cut(s) 452, 810
Fnu4HI GCNGC 5 cut(s) 6, 333, 515, 668, 920
FokI GGATG 1 cut(s) 247
Fsp4HI GCNGC 5 cut(s) 6, 333, 515, 668, 920
FspBI CTAG 4 cut(s) 99, 264, 329, 336
GlaI GCGC 1 cut(s) 884
GluI GCNGC 5 cut(s) 6, 333, 515, 668, 920
GsuI CTGGAG 1 cut(s) 968
HaeIII GGCC 1 cut(s) 514
HapII CCGG 1 cut(s) 622
HhaI GCGC 1 cut(s) 885
Hin1I GRCGYC 1 cut(s) 449
Hin1II CATG 1 cut(s) 513
Hin6I GCGC 1 cut(s) 883
HinP1I GCGC 1 cut(s) 883
HincII GTYRAC 2 cut(s) 412, 819
HindII GTYRAC 2 cut(s) 412, 819
HindIII AAGCTT 2 cut(s) 128, 800
HinfI GANTC 7 cut(s) 118, 147, 430, 876, 892, 948, 953
HpaI GTTAAC 1 cut(s) 819
HpaII CCGG 1 cut(s) 622
Hpy166II GTNNAC 4 cut(s) 412, 453, 811, 819
Hpy188I TCNGA 7 cut(s) 234, 343, 358, 465, 604, 771, 827
Hpy188III TCNNGA 7 cut(s) 144, 197, 434, 527, 761, 782, 868
Hpy8I GTNNAC 4 cut(s) 412, 453, 811, 819
HpyAV CCTTC 5 cut(s) 156, 364, 569, 584, 896
HpyCH4III ACNGT 3 cut(s) 220, 397, 416
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 4 cut(s) 39, 182, 257, 670
HpyF3I CTNAG 3 cut(s) 248, 615, 783
HpySE526I ACGT 1 cut(s) 449
Hsp92I GRCGYC 1 cut(s) 449
Hsp92II CATG 1 cut(s) 513
HspAI GCGC 1 cut(s) 883
Ksp22I TGATCA 1 cut(s) 771
KspAI GTTAAC 1 cut(s) 819
Kzo9I GATC 5 cut(s) 174, 193, 229, 523, 771
LmnI GCTCC 2 cut(s) 624, 784
Lsp1109I GCAGC 3 cut(s) 344, 654, 931
LweI GCATC 3 cut(s) 193, 232, 244
MaeI CTAG 4 cut(s) 99, 264, 329, 336
MaeII ACGT 1 cut(s) 449
MalI GATC 5 cut(s) 176, 195, 231, 525, 773
MboI GATC 5 cut(s) 174, 193, 229, 523, 771
MboII GAAGA 8 cut(s) 105, 212, 226, 289, 371, 512, 647, 671
MflI RGATCY 2 cut(s) 193, 523
MluCI AATT 6 cut(s) 40, 555, 581, 924, 969, 977
MlyI GAGTC 4 cut(s) 127, 141, 424, 962
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 4 cut(s) 304, 373, 713, 778
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 5 cut(s) 210, 476, 609, 818, 991
MspI CCGG 1 cut(s) 622
MspR9I CCNGG 3 cut(s) 651, 958, 986
MvaI CCWGG 3 cut(s) 651, 958, 986
MvnI CGCG 1 cut(s) 66
NdeII GATC 5 cut(s) 174, 193, 229, 523, 771
NlaIII CATG 1 cut(s) 513
NmeAIII GCCGAG 1 cut(s) 775
NspV TTCGAA 1 cut(s) 895
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 3 cut(s) 876, 892, 948
PkrI GCNGC 5 cut(s) 7, 334, 516, 669, 921
Ple19I CGATCG 1 cut(s) 232
PleI GAGTC 4 cut(s) 126, 141, 424, 961
PpsI GAGTC 4 cut(s) 126, 141, 424, 961
Psp6I CCWGG 3 cut(s) 649, 956, 984
PspGI CCWGG 3 cut(s) 649, 956, 984
PsuI RGATCY 2 cut(s) 193, 523
PvuI CGATCG 1 cut(s) 232
SaqAI TTAA 5 cut(s) 210, 476, 609, 818, 991
SatI GCNGC 5 cut(s) 6, 333, 515, 668, 920
Sau3AI GATC 5 cut(s) 174, 193, 229, 523, 771
SchI GAGTC 4 cut(s) 127, 141, 424, 962
ScrFI CCNGG 3 cut(s) 651, 958, 986
SfaNI GCATC 3 cut(s) 193, 232, 244
SfcI CTRYAG 2 cut(s) 405, 486
SfuI TTCGAA 1 cut(s) 895
Sse9I AATT 6 cut(s) 40, 555, 581, 924, 969, 977
SsiI CCGC 2 cut(s) 5, 515
SspMI CTAG 4 cut(s) 99, 264, 329, 336
StyD4I CCNGG 3 cut(s) 649, 956, 984
TaaI ACNGT 3 cut(s) 220, 397, 416
TaiI ACGT 1 cut(s) 452
TaqI TCGA 6 cut(s) 116, 222, 228, 879, 895, 951
TaqII GACCGA 1 cut(s) 947
TasI AATT 6 cut(s) 40, 555, 581, 924, 969, 977
TauI GCSGC 2 cut(s) 8, 517
TfiI GAWTC 3 cut(s) 876, 892, 948
Tru1I TTAA 5 cut(s) 210, 476, 609, 818, 991
Tru9I TTAA 5 cut(s) 210, 476, 609, 818, 991
TscAI CASTG 1 cut(s) 894
TseI GCWGC 3 cut(s) 332, 667, 919
TspDTI ATGAA 6 cut(s) 498, 560, 603, 900, 981, 989
TspRI CASTG 1 cut(s) 894
XapI RAATTY 1 cut(s) 924
XmiI GTMKAC 2 cut(s) 452, 810
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 4 cut(s) 99, 264, 329, 336
ZraI GACGTC 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.