MD10G1066100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011302
Physical Location & Seq
Forward (+)
9040875 .. 9046879
6005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1066100.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCTTCCTCATCATTACTCACGCCCGCGCCTCTGTCCGTACAAGTTCACCGGTGCTCCTCTTCACATTCTCACTCCCACCAGGTGCTTTGCTGCATTGGTTTAACAACGTCATTTCTCAAGAAATGGGATGTTGCGGGGATGTTGTGGAGGCCAAAGCCGTTCGGTTCGAACAATCATACGGATTGGAAGAAAAGGAGAACTTTGATCTGTGTAGCAAATCAAGATGCTGAAGAAGCATTCAAAAAGACTGTAGAAGTGGATAGGCTGATAGACAAACTGAGGGATTCAAATCCAATTGAACTTCAGAAGCTTGTTGTTCAAAATGTACTGGCTTTTAATGAGGGTTTTTGGATACGGCTTGCGGCAAGAACTGATACCTGTAAATCGGAGGATGATAAAAAAGACTACGAGGAGTTGGCAATATCTATTATGAGCATAGTGGATCGCCTTGTCCATAAGACTAATGAAAAGATAGATTCAGCTACTGATGTCCTCAAAGGGGTTCTGAAACCTGTAGTGGATGATGTAGAAGAAATTCGATGGCCCCCTAGAGATCCTGAAGCTCTTAAGTTAATGGAGAAAGAAATATTCCACAGGGAGCAAGAAGGGCAACTAGATGAAGGCTTTCTTGCAGAAGTTAATGCACAACTACGACAAGCGAAAGGAGATGGGGATAAGCCAGGGTTTGAGGCTATGTTACAAAAGGTTTTGCAGCTCTATGCTTCTACTGTTCTATCTAAACGTAGCTATGCAAAGAAAGGGGATGAAATTCTGAAGCCCGAGAAGCTTCTTGAAACCATAATCAAAGCTCCTGAGGAAGAATGGAACAAGCTGCTGATCAATGGATTGACAATTGGCAAAGGGGAAGTTTCTCCGCAGGAACTCTATGCTGTCATTAAGAAAAGGGTTGAGCGGACTCTGATCCGAACAGAGGGTGGTTCATACCAGCAGCGTATTCTTACGGAGTATTTGAATGGAATCCAATCCAGAGCGGAGCAAATTGTCCAAGTACTTCAGGGCAATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

38.93

Weight (kDa)

6.69

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 916, 995
AccII CGCG 1 cut(s) 29
AciI CCGC 6 cut(s) 27, 137, 365, 878, 916, 995
AclWI GGATC 3 cut(s) 453, 551, 919
AcsI RAATTY 2 cut(s) 537, 771
AcuI CTGAAG 5 cut(s) 252, 290, 582, 797, 1001
AdeI CACNNNGTG 1 cut(s) 85
AfaI GTAC 3 cut(s) 42, 330, 1014
AfiI CCNNNNNNNGG 2 cut(s) 502, 934
AflII CTTAAG 1 cut(s) 569
AgeI ACCGGT 1 cut(s) 51
AgsI TTSAA 6 cut(s) 244, 291, 302, 323, 797, 976
AjnI CCWGG 2 cut(s) 81, 682
AluBI AGCT 8 cut(s) 313, 485, 566, 718, 750, 790, 812, 835
AluI AGCT 8 cut(s) 313, 485, 566, 718, 750, 790, 812, 835
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 3 cut(s) 453, 551, 919
AlwNI CAGNNNCTG 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 782
AoxI GGCC 2 cut(s) 152, 545
ApeKI GCWGC 4 cut(s) 93, 715, 835, 952
ApoI RAATTY 2 cut(s) 537, 771
AsiGI ACCGGT 1 cut(s) 51
Asp700I GAANNNNTTC 2 cut(s) 537, 627
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 546
AsuHPI GGTGA 1 cut(s) 41
AsuII TTCGAA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 782
AxyI CCTNAGG 1 cut(s) 816
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 4 cut(s) 80, 727, 822, 964
BccI CCATC 2 cut(s) 537, 665
BceAI ACGGC 2 cut(s) 145, 374
BciT130I CCWGG 2 cut(s) 83, 684
BciVI GTATCC 1 cut(s) 348
BclI TGATCA 1 cut(s) 840
BfaI CTAG 2 cut(s) 552, 617
BfmI CTRYAG 3 cut(s) 252, 516, 1028
BfrI CTTAAG 1 cut(s) 569
BfuI GTATCC 1 cut(s) 348
BisI GCNGC 5 cut(s) 94, 366, 716, 836, 953
BlsI GCNGC 5 cut(s) 95, 367, 717, 837, 954
BmcAI AGTACT 1 cut(s) 1014
Bme1390I CCNGG 2 cut(s) 83, 684
BmeT110I CYCGRG 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 546
BmiI GGNNCC 1 cut(s) 548
BmrFI CCNGG 2 cut(s) 83, 684
BmsI GCATC 1 cut(s) 217
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 104
BsaBI GATNNNNATC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 683
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 934
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 336
Bse21I CCTNAGG 1 cut(s) 816
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 2 cut(s) 83, 684
BseDI CCNNGG 1 cut(s) 683
BseGI GGATG 5 cut(s) 136, 147, 400, 529, 772
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 2 cut(s) 502, 934
BseMII CTCAG 2 cut(s) 272, 807
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 2 cut(s) 49, 428
BseXI GCAGC 4 cut(s) 80, 727, 822, 964
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 2 cut(s) 154, 547
BshTI ACCGGT 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 782
BsiSI CCGG 1 cut(s) 52
BslI CCNNNNNNNGG 2 cut(s) 502, 934
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 2 cut(s) 154, 547
BsoBI CYCGRG 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 5 cut(s) 207, 445, 556, 840, 924
BspACI CCGC 6 cut(s) 27, 137, 365, 878, 916, 995
BspANI GGCC 2 cut(s) 154, 547
BspCNI CTCAG 2 cut(s) 273, 808
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 548
BspPI GGATC 3 cut(s) 453, 551, 919
BspT104I TTCGAA 1 cut(s) 170
BspTI CTTAAG 1 cut(s) 569
BsrBI CCGCTC 2 cut(s) 916, 995
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 1 cut(s) 336
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 683
BssMI GATC 5 cut(s) 207, 445, 556, 840, 924
Bst2UI CCWGG 2 cut(s) 83, 684
Bst4CI ACNGT 2 cut(s) 253, 733
Bst6I CTCTTC 1 cut(s) 67
BstAFI CTTAAG 1 cut(s) 569
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 27, 363
BstDEI CTNAG 2 cut(s) 281, 816
BstF5I GGATG 5 cut(s) 136, 147, 400, 529, 772
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 5 cut(s) 210, 448, 559, 843, 927
BstMBI GATC 5 cut(s) 207, 445, 556, 840, 924
BstMWI GCNNNNNNNGC 3 cut(s) 236, 610, 787
BstNI CCWGG 2 cut(s) 83, 684
BstSCI CCNGG 2 cut(s) 81, 682
BstSFI CTRYAG 3 cut(s) 252, 516, 1028
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 4 cut(s) 80, 727, 822, 964
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
Bsu36I CCTNAGG 1 cut(s) 816
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 2 cut(s) 154, 547
BtsCI GGATG 5 cut(s) 136, 147, 400, 529, 772
Cac8I GCNNGC 2 cut(s) 27, 363
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 546
CsiI ACCWGGT 1 cut(s) 81
Csp6I GTAC 3 cut(s) 41, 329, 1013
CspAI ACCGGT 1 cut(s) 51
CviQI GTAC 3 cut(s) 41, 329, 1013
DdeI CTNAG 2 cut(s) 281, 816
DpnI GATC 5 cut(s) 209, 447, 558, 842, 926
DpnII GATC 5 cut(s) 207, 445, 556, 840, 924
DraIII CACNNNGTG 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco57I CTGAAG 5 cut(s) 252, 290, 582, 797, 1001
Eco81I CCTNAGG 1 cut(s) 816
Eco88I CYCGRG 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 81, 682
FalI AAGNNNNNCTT 4 cut(s) 187, 219, 615, 647
FauI CCCGC 2 cut(s) 34, 130
FbaI TGATCA 1 cut(s) 840
Fnu4HI GCNGC 5 cut(s) 94, 366, 716, 836, 953
FokI GGATG 5 cut(s) 143, 154, 407, 536, 779
Fsp4HI GCNGC 5 cut(s) 94, 366, 716, 836, 953
FspBI CTAG 2 cut(s) 552, 617
GlaI GCGC 1 cut(s) 30
GluI GCNGC 5 cut(s) 94, 366, 716, 836, 953
HaeIII GGCC 2 cut(s) 154, 547
HapII CCGG 1 cut(s) 52
HhaI GCGC 1 cut(s) 31
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HindIII AAGCTT 2 cut(s) 311, 788
HinfI GANTC 4 cut(s) 287, 479, 919, 981
HpaII CCGG 1 cut(s) 52
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 6 cut(s) 309, 391, 510, 777, 924, 929
Hpy188III TCNNGA 6 cut(s) 121, 224, 560, 794, 815, 990
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 2 cut(s) 602, 617
HpyCH4III ACNGT 2 cut(s) 253, 733
HpyCH4IV ACGT 2 cut(s) 110, 745
HpyCH4V TGCA 5 cut(s) 96, 635, 647, 715, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 610, 787
HpyF3I CTNAG 2 cut(s) 281, 816
HpySE526I ACGT 2 cut(s) 110, 745
HspAI GCGC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 5 cut(s) 207, 445, 556, 840, 924
LmnI GCTCC 4 cut(s) 62, 601, 817, 997
Lsp1109I GCAGC 4 cut(s) 80, 727, 822, 964
LweI GCATC 1 cut(s) 217
MabI ACCWGGT 1 cut(s) 81
MaeI CTAG 2 cut(s) 552, 617
MaeII ACGT 2 cut(s) 110, 745
MaeIII GTNAC 1 cut(s) 699
MalI GATC 5 cut(s) 209, 447, 558, 842, 926
MbiI CCGCTC 2 cut(s) 916, 995
MboI GATC 5 cut(s) 207, 445, 556, 840, 924
MboII GAAGA 5 cut(s) 54, 202, 245, 545, 833
MfeI CAATTG 2 cut(s) 297, 855
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 5 cut(s) 297, 537, 771, 855, 1002
MlyI GAGTC 1 cut(s) 913
MroXI GAANNNNTTC 2 cut(s) 537, 627
MseI TTAA 6 cut(s) 104, 339, 570, 575, 642, 900
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 2 cut(s) 83, 684
MunI CAATTG 2 cut(s) 297, 855
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 2 cut(s) 83, 684
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 236, 610, 787
NdeII GATC 5 cut(s) 207, 445, 556, 840, 924
NlaIV GGNNCC 1 cut(s) 548
NspV TTCGAA 1 cut(s) 170
PctI GAATGC 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 537, 627
PfeI GAWTC 3 cut(s) 287, 479, 981
PinAI ACCGGT 1 cut(s) 51
PkrI GCNGC 5 cut(s) 95, 367, 717, 837, 954
PleI GAGTC 1 cut(s) 913
PpsI GAGTC 1 cut(s) 913
Psp6I CCWGG 2 cut(s) 81, 682
PspGI CCWGG 2 cut(s) 81, 682
PspN4I GGNNCC 1 cut(s) 548
PspPI GGNCC 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 556
RsaI GTAC 3 cut(s) 42, 330, 1014
RsaNI GTAC 3 cut(s) 41, 329, 1013
SaqAI TTAA 6 cut(s) 104, 339, 570, 575, 642, 900
SatI GCNGC 5 cut(s) 94, 366, 716, 836, 953
Sau3AI GATC 5 cut(s) 207, 445, 556, 840, 924
Sau96I GGNCC 1 cut(s) 546
ScaI AGTACT 1 cut(s) 1014
SchI GAGTC 1 cut(s) 913
ScrFI CCNGG 2 cut(s) 83, 684
SduI GDGCHC 1 cut(s) 59
SexAI ACCWGGT 1 cut(s) 81
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 3 cut(s) 252, 516, 1028
SfuI TTCGAA 1 cut(s) 170
SgrAI CRCCGGYG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 119, 569
SmoI CTYRAG 2 cut(s) 119, 569
Sse9I AATT 5 cut(s) 297, 537, 771, 855, 1002
SsiI CCGC 6 cut(s) 27, 137, 365, 878, 916, 995
SspI AATATT 1 cut(s) 591
SspMI CTAG 2 cut(s) 552, 617
StyD4I CCNGG 2 cut(s) 81, 682
TaaI ACNGT 2 cut(s) 253, 733
TaiI ACGT 2 cut(s) 113, 748
TaqI TCGA 2 cut(s) 170, 541
TasI AATT 5 cut(s) 297, 537, 771, 855, 1002
TatI WGTACW 2 cut(s) 328, 1012
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 3 cut(s) 287, 479, 981
Tru1I TTAA 6 cut(s) 104, 339, 570, 575, 642, 900
Tru9I TTAA 6 cut(s) 104, 339, 570, 575, 642, 900
TseI GCWGC 4 cut(s) 93, 715, 835, 952
TspDTI ATGAA 4 cut(s) 483, 636, 783, 933
TspGWI ACGGA 3 cut(s) 28, 197, 980
Vha464I CTTAAG 1 cut(s) 569
XapI RAATTY 2 cut(s) 537, 771
XmnI GAANNNNTTC 2 cut(s) 537, 627
XspI CTAG 2 cut(s) 552, 617
ZrmI AGTACT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.