AT2G21950

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010182
Physical Location & Seq
Reverse (-)
9353174 .. 9354645
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21950.1

Sequence Viewer

Length: 1119 bp
ATGGCGGCTACAACGAGCTCCGGCGACGAACCACCGGAAACAAAATCACCGGCGCAATTGATTCCGTTGTTATCAGAAGACGTTGCTCTCAGTTGCCTTGCTCGCGTCCCTAGATGTCACTATCCAATCCTCTCACTCGTCTCCAAGACTTTCCGATCTCTCCCAACATCACCTCTTCTCTACGCCACGAGAGCTCTCGTCGGAGCCACTGAAAACATCCTCTACGTAGCAATCCGTATACCTCCTGAATCTGGTGCGTGTTGGTTCACTCTCCTCCATCGAACCTTATCGAACTCAACGAATTCGAAAATGCTTGTTCCAATCCCATCTTGTCCTTCACCGTCTCTAGTCGGATCCGCTTACGTTGTGGTAGATTCAGAGATTTACGTGATCGGTGGATCTATTAGAGACGTGCCTTCTTCGAGCGTGTGGGTTCTCGATTGCAGATTTCATACTTGGCGACGAGTCTCTAATATGAGAGTAGGTCGTGAATTTGCAGCAGCTGGTGTAATTGATGGGAAGATTTATGTGATTGGAGGATGTGTAGTTGATAATTGGGCTCGATCGATTAACTGGGCAGAGATGTTTGATATAAAGACTCAAACTTGGGAGCCAGTGGCTAGTCCAGGTATGGAGGTTCGTGAGAAATGGATGCACGCAAGTGCGGTTATGGAAGGGAAGGTTTACGCAATGGCTGATCGAAACGGTGTTGTTTACGAACCCAAGGAAAAGAAATGGGAGATGCCTGAGAAGAGGCTTGATTTGGGATGGAGAGGAAGAGCTTGTGTGATTGAGAACATATTGTATTGTTATGATTACTTGGGGAAGATTAGAGGGTATGATCCAAAAGAGAGGATTTGGAGAGAGCTGAAAGGTGTGGAGTCACTTCCAAAGTTTCTTTGTGGGGCTACAATGGCTAATCGTGGTGGAAAGCTTACGGTTTTGTGGGAAGGTAAAGCTGGTAGTGGTGGTTCTAGGAGAATGGAGATTTGGTGTGCGGAGATTGATGTTGGAAGGCGTGGAGAAAGGGAGATTTGGGGAAAAATTGAATGGTCTGGTACCGTGCTTACGGTTCCTAATGAATCTGCCATTGTGAATTGCTTGGCAGCTACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

372

Amino Acids

41.29

Weight (kDa)

8.57

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 61 5.4e-06 F-box domain
Kelch_FKB95 PF25210 88 - 356 5.3e-101 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 88 - 203 5.5e-09 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 108 - 240 2.6e-15 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 114 - 224 2e-07 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 117 - 159 1.6e-06 Kelch motif
Kelch_1 PF01344 118 - 160 8.7e-13 Kelch motif
Beta-prop_Calicin PF13964 121 - 236 8.1e-09 Calicin, beta-propeller domain
Kelch_1 PF01344 163 - 209 1.9e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1058
AccB1I GGYRCC 1 cut(s) 1058
AccI GTMKAC 1 cut(s) 238
AccII CGCG 1 cut(s) 105
AciI CCGC 4 cut(s) 5, 357, 665, 998
AclWI GGATC 4 cut(s) 348, 361, 406, 836
AcsI RAATTY 2 cut(s) 301, 491
AfaI GTAC 1 cut(s) 1060
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 1 cut(s) 1049
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 1 cut(s) 625
AjuI GAANNNNNNNTTGG 2 cut(s) 973, 1005
AleI CACNNNNGTG 1 cut(s) 660
AluBI AGCT 8 cut(s) 18, 194, 503, 782, 868, 934, 959, 1109
AluI AGCT 8 cut(s) 18, 194, 503, 782, 868, 934, 959, 1109
Alw21I GWGCWC 2 cut(s) 20, 196
Alw26I GTCTC 4 cut(s) 145, 348, 402, 472
AlwI GGATC 4 cut(s) 348, 361, 406, 836
AlwNI CAGNNNCTG 1 cut(s) 503
ApeKI GCWGC 3 cut(s) 497, 500, 1106
ApoI RAATTY 2 cut(s) 301, 491
Asp718I GGTACC 1 cut(s) 1058
AspLEI GCGC 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 39, 162, 330
AsuII TTCGAA 1 cut(s) 305
BamHI GGATCC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 1058
BanII GRGCYC 3 cut(s) 20, 196, 562
BauI CACGAG 1 cut(s) 187
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 20, 196
BbvI GCAGC 2 cut(s) 509, 512
BccI CCATC 4 cut(s) 285, 334, 509, 762
BciT130I CCWGG 1 cut(s) 627
BcoDI GTCTC 4 cut(s) 145, 348, 402, 472
BfaI CTAG 4 cut(s) 111, 347, 621, 975
BfmI CTRYAG 1 cut(s) 1110
BisI GCNGC 4 cut(s) 6, 498, 501, 1107
BlsI GCNGC 4 cut(s) 7, 499, 502, 1108
Bme1390I CCNGG 1 cut(s) 627
BmgBI CACGTC 1 cut(s) 412
BmiI GGNNCC 5 cut(s) 205, 355, 612, 1060, 1074
BmrFI CCNGG 1 cut(s) 627
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 2 cut(s) 642, 732
BmuI ACTGGG 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 566
BsaAI YACGTR 2 cut(s) 226, 388
BsaJI CCNNGG 1 cut(s) 723
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 4 cut(s) 528, 558, 977, 1007
Bsc4I CCNNNNNNNGG 1 cut(s) 251
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 578, 614
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 566
BseDI CCNNGG 1 cut(s) 723
BseGI GGATG 4 cut(s) 216, 545, 657, 773
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 2 cut(s) 103, 738
BseNI ACTGG 2 cut(s) 578, 614
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 509, 512
Bsh1236I CGCG 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 566
BshNI GGYRCC 1 cut(s) 1058
BshVI ATCGAT 1 cut(s) 566
BsiEI CGRYCG 1 cut(s) 566
BsiHKAI GWGCWC 2 cut(s) 20, 196
BsiSI CCGG 3 cut(s) 21, 35, 50
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 4 cut(s) 145, 348, 402, 472
BsmBI CGTCTC 3 cut(s) 145, 348, 402
BsmFI GGGAC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 20, 196, 562
Bsp143I GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
BspACI CCGC 4 cut(s) 5, 357, 665, 998
BspCNI CTCAG 2 cut(s) 102, 739
BspDI ATCGAT 1 cut(s) 566
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 5 cut(s) 205, 355, 612, 1060, 1074
BspPI GGATC 4 cut(s) 348, 361, 406, 836
BspQI GCTCTTC 1 cut(s) 772
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 1058
BsrDI GCAATG 1 cut(s) 696
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 2 cut(s) 578, 614
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 723
BssMI GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
BssNAI GTATAC 1 cut(s) 239
BssSI CACGAG 1 cut(s) 187
BssT1I CCWWGG 1 cut(s) 723
Bst1107I GTATAC 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 6 cut(s) 342, 707, 940, 1063, 1072, 1114
Bst6I CTCTTC 3 cut(s) 180, 746, 772
BstBAI YACGTR 2 cut(s) 226, 388
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 103, 657
BstDEI CTNAG 2 cut(s) 89, 747
BstF5I GGATG 4 cut(s) 216, 545, 657, 773
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 7 cut(s) 158, 356, 393, 401, 566, 700, 844
BstMAI GTCTC 4 cut(s) 145, 348, 402, 472
BstMBI GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
BstMCI CGRYCG 1 cut(s) 566
BstMWI GCNNNNNNNGC 3 cut(s) 102, 191, 914
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 625
BstSFI CTRYAG 1 cut(s) 1110
BstSNI TACGTA 1 cut(s) 226
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 509, 512
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 353, 398
BstYI RGATCY 2 cut(s) 353, 398
BstZ17I GTATAC 1 cut(s) 239
Bsu15I ATCGAT 1 cut(s) 566
BsuTUI ATCGAT 1 cut(s) 566
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 4 cut(s) 216, 545, 657, 773
BtsIMutI CAGTG 2 cut(s) 207, 621
Cac8I GCNNGC 2 cut(s) 103, 657
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 49
ClaI ATCGAT 1 cut(s) 566
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 1059
CviQI GTAC 1 cut(s) 1059
DdeI CTNAG 2 cut(s) 89, 747
DpnI GATC 7 cut(s) 157, 355, 392, 400, 565, 699, 843
DpnII GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
Eam1104I CTCTTC 3 cut(s) 180, 746, 772
EarI CTCTTC 3 cut(s) 180, 746, 772
Ecl136II GAGCTC 2 cut(s) 18, 194
Eco105I TACGTA 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 723
Eco24I GRGCYC 3 cut(s) 20, 196, 562
Eco53kI GAGCTC 2 cut(s) 18, 194
EcoICRI GAGCTC 2 cut(s) 18, 194
EcoRI GAATTC 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 723
EcoT38I GRGCYC 3 cut(s) 20, 196, 562
ErhI CCWWGG 1 cut(s) 723
Esp3I CGTCTC 3 cut(s) 145, 348, 402
FaqI GGGAC 1 cut(s) 92
FblI GTMKAC 1 cut(s) 238
Fnu4HI GCNGC 4 cut(s) 6, 498, 501, 1107
FokI GGATG 4 cut(s) 203, 552, 664, 780
FriOI GRGCYC 3 cut(s) 20, 196, 562
Fsp4HI GCNGC 4 cut(s) 6, 498, 501, 1107
FspBI CTAG 4 cut(s) 111, 347, 621, 975
GlaI GCGC 1 cut(s) 54
GluI GCNGC 4 cut(s) 6, 498, 501, 1107
HapII CCGG 3 cut(s) 21, 35, 50
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 55
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 7 cut(s) 61, 248, 374, 465, 598, 881, 1082
HpaII CCGG 3 cut(s) 21, 35, 50
HphI GGTGA 3 cut(s) 39, 162, 330
Hpy166II GTNNAC 4 cut(s) 239, 267, 685, 715
Hpy188I TCNGA 5 cut(s) 76, 155, 203, 353, 379
Hpy188III TCNNGA 4 cut(s) 245, 437, 488, 641
Hpy8I GTNNAC 4 cut(s) 239, 267, 685, 715
Hpy99I CGWCG 3 cut(s) 29, 203, 465
HpyAV CCTTC 6 cut(s) 345, 426, 668, 673, 944, 1008
HpyCH4III ACNGT 6 cut(s) 342, 707, 940, 1063, 1072, 1114
HpyCH4IV ACGT 5 cut(s) 81, 225, 363, 387, 411
HpyCH4V TGCA 3 cut(s) 444, 497, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 191, 914
HpyF3I CTNAG 2 cut(s) 89, 747
HpySE526I ACGT 5 cut(s) 81, 225, 363, 387, 411
HspAI GCGC 1 cut(s) 53
KpnI GGTACC 1 cut(s) 1062
Kzo9I GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
LguI GCTCTTC 1 cut(s) 772
LmnI GCTCC 3 cut(s) 23, 203, 610
Lsp1109I GCAGC 2 cut(s) 509, 512
LweI GCATC 2 cut(s) 642, 732
MaeI CTAG 4 cut(s) 111, 347, 621, 975
MaeII ACGT 5 cut(s) 81, 225, 363, 387, 411
MaeIII GTNAC 2 cut(s) 116, 882
MalI GATC 7 cut(s) 157, 355, 392, 400, 565, 699, 843
MboI GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
MboII GAAGA 7 cut(s) 89, 167, 411, 532, 763, 789, 838
MfeI CAATTG 1 cut(s) 56
MflI RGATCY 2 cut(s) 353, 398
MhlI GDGCHC 3 cut(s) 20, 196, 562
MluCI AATT 7 cut(s) 56, 301, 491, 510, 553, 1044, 1096
MlyI GAGTC 3 cut(s) 474, 592, 890
MmeI TCCRAC 3 cut(s) 181, 331, 991
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 3 cut(s) 21, 35, 50
MspR9I CCNGG 1 cut(s) 627
MunI CAATTG 1 cut(s) 56
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 3 cut(s) 102, 191, 914
NdeII GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
NlaIV GGNNCC 5 cut(s) 205, 355, 612, 1060, 1074
NmuCI GTSAC 2 cut(s) 116, 882
NspV TTCGAA 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 772
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PfeI GAWTC 4 cut(s) 61, 248, 374, 1082
PkrI GCNGC 4 cut(s) 7, 499, 502, 1108
Ple19I CGATCG 1 cut(s) 566
PleI GAGTC 3 cut(s) 473, 592, 889
PpsI GAGTC 3 cut(s) 473, 592, 889
Ppu21I YACGTR 2 cut(s) 226, 388
Psp124BI GAGCTC 2 cut(s) 20, 196
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 5 cut(s) 205, 355, 612, 1060, 1074
PsrI GAACNNNNNNTAC 2 cut(s) 955, 987
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 2 cut(s) 353, 398
PvuI CGATCG 1 cut(s) 566
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 1060
RsaNI GTAC 1 cut(s) 1059
RseI CAYNNNNRTG 1 cut(s) 660
SacI GAGCTC 2 cut(s) 20, 196
SapI GCTCTTC 1 cut(s) 772
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 4 cut(s) 6, 498, 501, 1107
Sau3AI GATC 7 cut(s) 155, 353, 390, 398, 563, 697, 841
SchI GAGTC 3 cut(s) 474, 592, 890
ScrFI CCNGG 1 cut(s) 627
SduI GDGCHC 3 cut(s) 20, 196, 562
SfaNI GCATC 2 cut(s) 642, 732
SfcI CTRYAG 1 cut(s) 1110
SfuI TTCGAA 1 cut(s) 305
SgrAI CRCCGGYG 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 660
SnaBI TACGTA 1 cut(s) 226
Sse9I AATT 7 cut(s) 56, 301, 491, 510, 553, 1044, 1096
SsiI CCGC 4 cut(s) 5, 357, 665, 998
SspMI CTAG 4 cut(s) 111, 347, 621, 975
SstI GAGCTC 2 cut(s) 20, 196
StyD4I CCNGG 1 cut(s) 625
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 6 cut(s) 342, 707, 940, 1063, 1072, 1114
TaiI ACGT 5 cut(s) 84, 228, 366, 390, 414
TaqI TCGA 8 cut(s) 280, 290, 305, 422, 438, 562, 566, 700
TasI AATT 7 cut(s) 56, 301, 491, 510, 553, 1044, 1096
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 61, 248, 374, 1082
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 2 cut(s) 214, 621
TseFI GTSAC 2 cut(s) 116, 882
TseI GCWGC 3 cut(s) 497, 500, 1106
Tsp45I GTSAC 2 cut(s) 116, 882
TspDTI ATGAA 2 cut(s) 440, 1095
TspGWI ACGGA 2 cut(s) 54, 224
TspRI CASTG 2 cut(s) 214, 621
XapI RAATTY 2 cut(s) 301, 491
XmiI GTMKAC 1 cut(s) 238
XspI CTAG 4 cut(s) 111, 347, 621, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.