FvH4_1g22291

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010182
Physical Location & Seq
Forward (+)
14189964 .. 14192302
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22291.t1

Sequence Viewer

Length: 1104 bp
ATGTCCACCACCTCCACCACTGAAGCCACCGCAACGGCCTCCGCATCCGAATCGGAGCTAATCCCCTCCCTCCCATACGACGTCGCTTCAAACTGCCTAGCTCGAATCCCACGCCACTACCACCCAACCCTCTCAGTCCTATCCAAACCCATCCGCTCTCTCCTCTCTTCCCCTTTCTTCTTCACCACCCGCTCCCTCCTCAACTCCGCCCAACACCTCCTCTACCTCTCCCTCCGCTCCTCGGACCACTGCTCCACCTGGTTCGCCCTCTACCAAAACCCCAACCCCCAAAGCGACGCCGCTCGCCTCCTCGTCCCGATCCCTCCCGTCCCTATCCCCTCGGTCGGCGCCGCCTACGCCGTCGTCGGTTCTTCAATCTACGTCCTCGGCGGGTCCGTCAACGACGCGCCGTCCAATAATGTATGGATCCTCGACTGCCGCTTCAACACGTGGCGCGCCGGGCCGCCGATGCGGGTGTCGCGTGAGTTCGCCGCGGCCGGGGTTGTGGACGGGAGAGTGTACGTGATCGGCGGTTGCGTGACTGACTCGTGGACCAGGTCGGAGTATTGGGCCGAGGCTCTGGATCCGGGTACGGGTCGGTGGGAGGCGGTGGCGAGTCCGGTGGAGGTTCGGGGGAAGTGGATGCACGCGAGTGCGGTGATCGGCGGGAGGGTTTATGCGCTGGCGGACCGGGGCGGGGTGGTTTACGACCCGAAAACCCGGGTTTGGGAGGGAGTGGAGAAGAGGATGGATATGGGCTGGAGAGGGAGGGCTTGTGTTGTGCATGGGATGTTGTATTGTTATGATTATTTGGGGAAGATTAGAGGTTTCGATGCGAAGAATCGAGTGTGGAAGGAGCTTAAAGGAGTGGAGAAGAAGCTGCCGAAGTTCTTGTGCGGCGCAACTATGGCGAATATGGGGGAGAATTTGGTGGTTTTGTGGGAGACAAAGGCGAATGGGAAGGAGATGGAGATTTGGTGTGCTGAGATTGAGGTGAAGGAGAATGGGGATGGGGAGTTGTGGGGGAGAATCGATTGGTCACAGAAGCTTCTTTCGGTTCCTAAGCACTCCAACATTGTGCAATGCTCTGCGGTTGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

368

Amino Acids

40.48

Weight (kDa)

8.3

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 87 - 350 2.9e-82 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 107 - 231 6.2e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 109 - 250 2.1e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 109 - 194 8.4e-07 FBX42, beta-propeller domain
Kelch_1 PF01344 115 - 158 2.1e-11 Kelch motif
Kelch_1 PF01344 161 - 206 2.1e-09 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 347
AccBSI CCGCTC 4 cut(s) 156, 192, 237, 302
AccII CGCG 5 cut(s) 407, 456, 481, 494, 650
AclWI GGATC 5 cut(s) 313, 421, 434, 578, 591
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 1 cut(s) 925
AcuI CTGAAG 1 cut(s) 42
AcvI CACGTG 1 cut(s) 450
AcyI GRCGYC 3 cut(s) 81, 297, 348
AfaI GTAC 2 cut(s) 521, 592
AfiI CCNNNNNNNGG 7 cut(s) 241, 344, 498, 593, 697, 726, 727
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 3 cut(s) 90, 375, 445
AhdI GACNNNNNGTC 1 cut(s) 409
AjnI CCWGG 2 cut(s) 257, 554
AleI CACNNNNGTG 1 cut(s) 651
AluBI AGCT 5 cut(s) 58, 101, 859, 880, 1048
AluI AGCT 5 cut(s) 58, 101, 859, 880, 1048
Alw26I GTCTC 1 cut(s) 938
AlwI GGATC 5 cut(s) 313, 421, 434, 578, 591
Ama87I CYCGRG 1 cut(s) 720
AoxI GGCC 4 cut(s) 36, 461, 495, 570
ApeKI GCWGC 1 cut(s) 880
ApoI RAATTY 1 cut(s) 925
AscI GGCGCGCC 1 cut(s) 454
AspLEI GCGC 6 cut(s) 350, 409, 456, 458, 682, 902
AspS9I GGNCC 6 cut(s) 244, 393, 461, 552, 570, 688
AsuC2I CCSGG 6 cut(s) 460, 499, 588, 692, 721, 722
AsuHPI GGTGA 3 cut(s) 175, 670, 1006
AvaI CYCGRG 1 cut(s) 720
AvaII GGWCC 4 cut(s) 244, 393, 552, 688
BamHI GGATCC 2 cut(s) 426, 583
BanI GGYRCC 1 cut(s) 347
BauI CACGAG 1 cut(s) 547
BbrPI CACGTG 1 cut(s) 450
BbvI GCAGC 1 cut(s) 867
BccI CCATC 4 cut(s) 158, 742, 961, 1004
BceAI ACGGC 3 cut(s) 51, 344, 394
BcgI CGANNNNNNTGC 2 cut(s) 33, 67
BciT130I CCWGG 2 cut(s) 259, 556
BcnI CCSGG 6 cut(s) 460, 499, 588, 692, 721, 722
BcoDI GTCTC 1 cut(s) 938
BfaI CTAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 351
BisI GCNGC 8 cut(s) 300, 351, 439, 464, 492, 495, 881, 898
BlsI GCNGC 8 cut(s) 301, 352, 440, 465, 493, 496, 882, 899
Bme1390I CCNGG 8 cut(s) 259, 460, 499, 556, 588, 692, 721, 722
Bme18I GGWCC 4 cut(s) 244, 393, 552, 688
BmeRI GACNNNNNGTC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 720
BmgT120I GGNCC 6 cut(s) 244, 393, 461, 552, 570, 688
BmiI GGNNCC 5 cut(s) 349, 394, 428, 585, 1059
BmrFI CCNGG 8 cut(s) 259, 460, 499, 556, 588, 692, 721, 722
BmsI GCATC 4 cut(s) 53, 459, 633, 823
BpmI CTGGAG 1 cut(s) 781
Bpu10I CCTNAGC 1 cut(s) 1062
BpuMI CCSGG 6 cut(s) 460, 499, 588, 692, 721, 722
Bsa29I ATCGAT 1 cut(s) 1032
BsaAI YACGTR 2 cut(s) 450, 523
BsaHI GRCGYC 3 cut(s) 81, 297, 348
BsaJI CCNNGG 8 cut(s) 240, 339, 385, 492, 498, 573, 691, 720
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 6 cut(s) 236, 266, 760, 790, 962, 992
Bsc4I CCNNNNNNNGG 7 cut(s) 241, 344, 498, 593, 697, 726, 727
Bse3DI GCAATG 1 cut(s) 1088
BseBI CCWGG 2 cut(s) 259, 556
BseCI ATCGAT 1 cut(s) 1032
BseDI CCNNGG 8 cut(s) 240, 339, 385, 492, 498, 573, 691, 720
BseGI GGATG 6 cut(s) 44, 150, 648, 753, 795, 1015
BseLI CCNNNNNNNGG 7 cut(s) 241, 344, 498, 593, 697, 726, 727
BseMI GCAATG 1 cut(s) 1088
BseMII CTCAG 2 cut(s) 147, 975
BsePI GCGCGC 1 cut(s) 454
BseRI GAGGAG 5 cut(s) 152, 188, 209, 229, 299
BseX3I CGGCCG 1 cut(s) 495
BseXI GCAGC 1 cut(s) 867
Bsh1236I CGCG 5 cut(s) 407, 456, 481, 494, 650
Bsh1285I CGRYCG 2 cut(s) 345, 498
BshFI GGCC 4 cut(s) 38, 463, 497, 572
BshNI GGYRCC 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 1032
BsiEI CGRYCG 2 cut(s) 345, 498
BsiHKCI CYCGRG 1 cut(s) 720
BsiSI CCGG 6 cut(s) 459, 498, 587, 620, 691, 721
BslFI GGGAC 2 cut(s) 299, 314
BslI CCNNNNNNNGG 7 cut(s) 241, 344, 498, 593, 697, 726, 727
BsmAI GTCTC 1 cut(s) 938
BsmFI GGGAC 2 cut(s) 299, 314
BsnI GGCC 4 cut(s) 38, 463, 497, 572
BsoBI CYCGRG 1 cut(s) 720
Bsp143I GATC 5 cut(s) 318, 426, 525, 583, 660
BspANI GGCC 4 cut(s) 38, 463, 497, 572
BspCNI CTCAG 2 cut(s) 146, 976
BspDI ATCGAT 1 cut(s) 1032
BspFNI CGCG 5 cut(s) 407, 456, 481, 494, 650
BspLI GGNNCC 5 cut(s) 349, 394, 428, 585, 1059
BspPI GGATC 5 cut(s) 313, 421, 434, 578, 591
BspT107I GGYRCC 1 cut(s) 347
BsrBI CCGCTC 4 cut(s) 156, 192, 237, 302
BsrDI GCAATG 1 cut(s) 1088
BssECI CCNNGG 8 cut(s) 240, 339, 385, 492, 498, 573, 691, 720
BssHII GCGCGC 1 cut(s) 454
BssMI GATC 5 cut(s) 318, 426, 525, 583, 660
BssNI GRCGYC 3 cut(s) 81, 297, 348
BssSI CACGAG 1 cut(s) 547
Bst2BI CACGAG 1 cut(s) 547
Bst2UI CCWGG 2 cut(s) 259, 556
Bst6I CTCTTC 2 cut(s) 172, 737
BstACI GRCGYC 3 cut(s) 81, 297, 348
BstBAI YACGTR 2 cut(s) 450, 523
BstC8I GCNNGC 4 cut(s) 304, 456, 648, 684
BstDEI CTNAG 3 cut(s) 133, 984, 1062
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 6 cut(s) 44, 150, 648, 753, 795, 1015
BstFNI CGCG 5 cut(s) 407, 456, 481, 494, 650
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 6 cut(s) 350, 409, 456, 458, 682, 902
BstKTI GATC 5 cut(s) 321, 429, 528, 586, 663
BstMAI GTCTC 1 cut(s) 938
BstMBI GATC 5 cut(s) 318, 426, 525, 583, 660
BstMCI CGRYCG 2 cut(s) 345, 498
BstMWI GCNNNNNNNGC 5 cut(s) 356, 460, 469, 478, 908
BstNI CCWGG 2 cut(s) 259, 556
BstSCI CCNGG 8 cut(s) 257, 458, 497, 554, 586, 690, 719, 720
BstUI CGCG 5 cut(s) 407, 456, 481, 494, 650
BstV1I GCAGC 1 cut(s) 867
BstX2I RGATCY 2 cut(s) 426, 583
BstYI RGATCY 2 cut(s) 426, 583
BstZI CGGCCG 1 cut(s) 495
Bsu15I ATCGAT 1 cut(s) 1032
BsuRI GGCC 4 cut(s) 38, 463, 497, 572
BsuTUI ATCGAT 1 cut(s) 1032
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 6 cut(s) 44, 150, 648, 753, 795, 1015
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 18, 247
Cac8I GCNNGC 4 cut(s) 304, 456, 648, 684
CfoI GCGC 6 cut(s) 350, 409, 456, 458, 682, 902
Cfr13I GGNCC 6 cut(s) 244, 393, 461, 552, 570, 688
Cfr42I CCGCGG 1 cut(s) 495
Cfr9I CCCGGG 1 cut(s) 720
ClaI ATCGAT 1 cut(s) 1032
CpoI CGGWCCG 1 cut(s) 688
CseI GACGC 2 cut(s) 305, 413
CsiI ACCWGGT 2 cut(s) 257, 554
Csp6I GTAC 2 cut(s) 520, 591
CspI CGGWCCG 1 cut(s) 688
CviAII CATG 1 cut(s) 785
CviQI GTAC 2 cut(s) 520, 591
DdeI CTNAG 3 cut(s) 133, 984, 1062
DinI GGCGCC 1 cut(s) 349
DpnI GATC 5 cut(s) 320, 428, 527, 585, 662
DpnII GATC 5 cut(s) 318, 426, 525, 583, 660
DriI GACNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 495
EagI CGGCCG 1 cut(s) 495
Eam1104I CTCTTC 2 cut(s) 172, 737
Eam1105I GACNNNNNGTC 1 cut(s) 409
EarI CTCTTC 2 cut(s) 172, 737
EciI GGCGGA 2 cut(s) 196, 701
EclXI CGGCCG 1 cut(s) 495
Eco47I GGWCC 4 cut(s) 244, 393, 552, 688
Eco52I CGGCCG 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 42
Eco72I CACGTG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 720
EcoRII CCWGG 2 cut(s) 257, 554
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
FaeI CATG 1 cut(s) 788
FaiI YATR 8 cut(s) 76, 424, 678, 755, 786, 804, 908, 917
FaqI GGGAC 2 cut(s) 299, 314
FatI CATG 1 cut(s) 784
FauI CCCGC 5 cut(s) 197, 383, 465, 659, 689
Fnu4HI GCNGC 8 cut(s) 300, 351, 439, 464, 492, 495, 881, 898
FokI GGATG 6 cut(s) 31, 137, 655, 760, 802, 1022
Fsp4HI GCNGC 8 cut(s) 300, 351, 439, 464, 492, 495, 881, 898
FspBI CTAG 1 cut(s) 98
GlaI GCGC 6 cut(s) 349, 408, 455, 457, 681, 901
GluI GCNGC 8 cut(s) 300, 351, 439, 464, 492, 495, 881, 898
GsuI CTGGAG 1 cut(s) 781
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 4 cut(s) 38, 463, 497, 572
HapII CCGG 6 cut(s) 459, 498, 587, 620, 691, 721
HgaI GACGC 2 cut(s) 305, 413
HhaI GCGC 6 cut(s) 350, 409, 456, 458, 682, 902
Hin1I GRCGYC 3 cut(s) 81, 297, 348
Hin1II CATG 1 cut(s) 788
Hin6I GCGC 6 cut(s) 348, 407, 454, 456, 680, 900
HinP1I GCGC 6 cut(s) 348, 407, 454, 456, 680, 900
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 6 cut(s) 50, 105, 545, 616, 841, 1029
HpaII CCGG 6 cut(s) 459, 498, 587, 620, 691, 721
HphI GGTGA 3 cut(s) 175, 670, 1006
Hpy166II GTNNAC 6 cut(s) 6, 400, 508, 520, 552, 706
Hpy188I TCNGA 4 cut(s) 49, 55, 244, 562
Hpy188III TCNNGA 2 cut(s) 316, 581
Hpy8I GTNNAC 6 cut(s) 6, 400, 508, 520, 552, 706
Hpy99I CGWCG 6 cut(s) 83, 86, 299, 365, 368, 407
HpyAV CCTTC 3 cut(s) 847, 955, 991
HpyCH4IV ACGT 4 cut(s) 81, 381, 449, 522
HpyCH4V TGCA 3 cut(s) 646, 784, 1081
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 460, 469, 478, 908
HpyF3I CTNAG 3 cut(s) 133, 984, 1062
HpySE526I ACGT 4 cut(s) 81, 381, 449, 522
Hsp92I GRCGYC 3 cut(s) 81, 297, 348
Hsp92II CATG 1 cut(s) 788
HspAI GCGC 6 cut(s) 348, 407, 454, 456, 680, 900
KasI GGCGCC 1 cut(s) 347
KspI CCGCGG 1 cut(s) 495
Kzo9I GATC 5 cut(s) 318, 426, 525, 583, 660
LmnI GCTCC 5 cut(s) 55, 197, 242, 257, 856
Lsp1109I GCAGC 1 cut(s) 867
LweI GCATC 4 cut(s) 53, 459, 633, 823
MabI ACCWGGT 2 cut(s) 257, 554
MaeI CTAG 1 cut(s) 98
MaeII ACGT 4 cut(s) 81, 381, 449, 522
MaeIII GTNAC 2 cut(s) 538, 1038
MalI GATC 5 cut(s) 320, 428, 527, 585, 662
MbiI CCGCTC 4 cut(s) 156, 192, 237, 302
MboI GATC 5 cut(s) 318, 426, 525, 583, 660
MboII GAAGA 8 cut(s) 159, 169, 172, 363, 754, 829, 850, 886
MflI RGATCY 2 cut(s) 426, 583
MluCI AATT 1 cut(s) 925
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 2 cut(s) 539, 625
MmeI TCCRAC 2 cut(s) 540, 1095
MseI TTAA 1 cut(s) 861
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 6 cut(s) 459, 498, 587, 620, 691, 721
MspR9I CCNGG 8 cut(s) 259, 460, 499, 556, 588, 692, 721, 722
MvaI CCWGG 2 cut(s) 259, 556
MvnI CGCG 5 cut(s) 407, 456, 481, 494, 650
MwoI GCNNNNNNNGC 5 cut(s) 356, 460, 469, 478, 908
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 6 cut(s) 460, 499, 588, 692, 721, 722
NdeII GATC 5 cut(s) 318, 426, 525, 583, 660
NlaIII CATG 1 cut(s) 788
NlaIV GGNNCC 5 cut(s) 349, 394, 428, 585, 1059
NmeAIII GCCGAG 2 cut(s) 366, 598
NmuCI GTSAC 2 cut(s) 538, 1038
OliI CACNNNNGTG 1 cut(s) 651
PalAI GGCGCGCC 1 cut(s) 454
PauI GCGCGC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 534
PfeI GAWTC 4 cut(s) 50, 105, 841, 1029
PflFI GACNNNGTC 1 cut(s) 556
PkrI GCNGC 8 cut(s) 301, 352, 440, 465, 493, 496, 882, 899
PleI GAGTC 2 cut(s) 539, 624
PluTI GGCGCC 1 cut(s) 351
PmaCI CACGTG 1 cut(s) 450
PmlI CACGTG 1 cut(s) 450
PpsI GAGTC 2 cut(s) 539, 624
Ppu21I YACGTR 2 cut(s) 450, 523
Psp6I CCWGG 2 cut(s) 257, 554
PspCI CACGTG 1 cut(s) 450
PspGI CCWGG 2 cut(s) 257, 554
PspN4I GGNNCC 5 cut(s) 349, 394, 428, 585, 1059
PspPI GGNCC 6 cut(s) 244, 393, 461, 552, 570, 688
PsuI RGATCY 2 cut(s) 426, 583
PsyI GACNNNGTC 1 cut(s) 556
PteI GCGCGC 1 cut(s) 454
RsaI GTAC 2 cut(s) 521, 592
RsaNI GTAC 2 cut(s) 520, 591
RseI CAYNNNNRTG 1 cut(s) 651
Rsr2I CGGWCCG 1 cut(s) 688
RsrII CGGWCCG 1 cut(s) 688
SacII CCGCGG 1 cut(s) 495
SaqAI TTAA 1 cut(s) 861
SatI GCNGC 8 cut(s) 300, 351, 439, 464, 492, 495, 881, 898
Sau3AI GATC 5 cut(s) 318, 426, 525, 583, 660
Sau96I GGNCC 6 cut(s) 244, 393, 461, 552, 570, 688
SchI GAGTC 2 cut(s) 539, 625
ScrFI CCNGG 8 cut(s) 259, 460, 499, 556, 588, 692, 721, 722
SexAI ACCWGGT 2 cut(s) 257, 554
SfaNI GCATC 4 cut(s) 53, 459, 633, 823
SfoI GGCGCC 1 cut(s) 349
Sfr303I CCGCGG 1 cut(s) 495
SgrBI CCGCGG 1 cut(s) 495
SgsI GGCGCGCC 1 cut(s) 454
SinI GGWCC 4 cut(s) 244, 393, 552, 688
SmaI CCCGGG 1 cut(s) 722
SmiMI CAYNNNNRTG 1 cut(s) 651
Sse9I AATT 1 cut(s) 925
SspDI GGCGCC 1 cut(s) 347
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 8 cut(s) 257, 458, 497, 554, 586, 690, 719, 720
TaiI ACGT 4 cut(s) 84, 384, 452, 525
TaqI TCGA 5 cut(s) 103, 432, 831, 844, 1032
TaqII GACCGA 1 cut(s) 331
TasI AATT 1 cut(s) 925
TauI GCSGC 7 cut(s) 302, 353, 441, 466, 494, 497, 900
TfiI GAWTC 4 cut(s) 50, 105, 841, 1029
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TscAI CASTG 2 cut(s) 25, 254
TseFI GTSAC 2 cut(s) 538, 1038
TseI GCWGC 1 cut(s) 880
Tsp45I GTSAC 2 cut(s) 538, 1038
TspGWI ACGGA 1 cut(s) 385
TspMI CCCGGG 1 cut(s) 720
TspRI CASTG 2 cut(s) 25, 254
Tth111I GACNNNGTC 1 cut(s) 556
VpaK11BI GGWCC 4 cut(s) 244, 393, 552, 688
XapI RAATTY 1 cut(s) 925
XmaI CCCGGG 1 cut(s) 720
XspI CTAG 1 cut(s) 98
ZraI GACGTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.