RLG00000018277

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010182
Physical Location & Seq
Forward (+)
27832207 .. 27833759
1553 bp
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UTR
Exon/CDS
Intron
RLM00000018277

Sequence Viewer

Length: 477 bp
ATGCGGAAGGAGCTGAAAGGAGTGGAGAAGAAGCTGCCGAAGTTCTTGTGTGCAACAATGGCAAATGTGGGGGAGAATTTGGTTGTTCTGTGGGAAACCAAGGGAAATGGGAAAGATACAGAGATTTGGTGTGCTGAGATTGAGGTGAAGGAAAATGGAGATGGGGAGGTGTGGGGGAGAATCGATTGGTCCCAGAAGATTCTTTCGGTTCCAAAACACTCCAACATTGTTCAATGCTCGGCGGTCGATGCACGAAGCCTACGAGGCCAGGATATTTATCTAGACTTTGAAAGGGATTTCTCAGAAGTAGTTGCTCGTCTCAACTCTCAAGAGGTTTCGTGGCAGAGTAACCACCCTGTATTCGCTATACAATGTGAGCTGCAGACATGTATAGAGGATACTAGCCCAGTGACATTTTCAGGCTCTGCAGGAGTTTTTGACATGAGGTCTAGCTTGCTTTCGGAATTACTGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

159

Amino Acids

17.8

Weight (kDa)

5.0

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 2 - 68 1.4e-14 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 4, 242
AcsI RAATTY 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 386
AgsI TTSAA 2 cut(s) 233, 290
AhdI GACNNNNNGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 4 cut(s) 13, 34, 379, 453
AluI AGCT 4 cut(s) 13, 34, 379, 453
Alw26I GTCTC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 34, 379
ApoI RAATTY 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 21, 366
BccI CCATC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 269
BciVI GTATCC 1 cut(s) 391
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 3 cut(s) 281, 402, 450
BfmI CTRYAG 2 cut(s) 380, 426
BfuI GTATCC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 264
BisI GCNGC 2 cut(s) 35, 380
BlsI GCNGC 2 cut(s) 36, 381
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 189
BmeRI GACNNNNNGTC 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 2 cut(s) 191, 210
BmrFI CCNGG 1 cut(s) 269
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 99
Bse1I ACTGG 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 269
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 99
BseJI GATNNNNATC 1 cut(s) 276
BseMII CTCAG 2 cut(s) 126, 315
BseNI ACTGG 1 cut(s) 407
BseXI GCAGC 2 cut(s) 21, 366
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 246
BslFI GGGAC 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 323
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 1 cut(s) 267
BspACI CCGC 2 cut(s) 4, 242
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 2 cut(s) 127, 314
BspDI ATCGAT 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 191, 210
BspMAI CTGCAG 2 cut(s) 384, 430
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 455
BstDEI CTNAG 2 cut(s) 135, 301
BstMAI GTCTC 1 cut(s) 323
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 4 cut(s) 10, 59, 248, 264
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 2 cut(s) 380, 426
BstV1I GCAGC 2 cut(s) 21, 366
Bsu15I ATCGAT 1 cut(s) 183
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 267
BsuTUI ATCGAT 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 455
CaiI CAGNNNCTG 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 189
ClaI ATCGAT 1 cut(s) 183
CviAII CATG 2 cut(s) 387, 442
CviJI RGCY 8 cut(s) 13, 34, 258, 267, 379, 405, 423, 453
CviKI_1 RGCY 8 cut(s) 13, 34, 258, 267, 379, 405, 423, 453
DdeI CTNAG 2 cut(s) 135, 301
DriI GACNNNNNGTC 1 cut(s) 445
Eam1105I GACNNNNNGTC 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 2 cut(s) 390, 445
FaiI YATR 4 cut(s) 368, 388, 392, 443
FaqI GGGAC 1 cut(s) 175
FatI CATG 2 cut(s) 386, 441
Fnu4HI GCNGC 2 cut(s) 35, 380
Fsp4HI GCNGC 2 cut(s) 35, 380
FspBI CTAG 3 cut(s) 281, 402, 450
GluI GCNGC 2 cut(s) 35, 380
HaeIII GGCC 1 cut(s) 267
Hin1II CATG 2 cut(s) 390, 445
HinfI GANTC 2 cut(s) 180, 199
HphI GGTGA 1 cut(s) 157
Hpy188I TCNGA 2 cut(s) 304, 463
Hpy188III TCNNGA 2 cut(s) 281, 329
HpyAV CCTTC 1 cut(s) 142
HpyCH4V TGCA 4 cut(s) 53, 251, 382, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 59, 248, 264
HpyF3I CTNAG 2 cut(s) 135, 301
Hsp92II CATG 2 cut(s) 390, 445
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 7 cut(s) 206, 254, 281, 369, 405, 414, 420
Lsp1109I GCAGC 2 cut(s) 21, 366
LweI GCATC 1 cut(s) 238
MaeI CTAG 3 cut(s) 281, 402, 450
MaeIII GTNAC 2 cut(s) 347, 409
MboII GAAGA 2 cut(s) 40, 208
MluCI AATT 2 cut(s) 76, 464
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 6 cut(s) 136, 160, 257, 325, 388, 438
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 4 cut(s) 10, 59, 248, 264
NlaIII CATG 2 cut(s) 390, 445
NlaIV GGNNCC 2 cut(s) 191, 210
NmeAIII GCCGAG 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 390
PciI ACATGT 1 cut(s) 386
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 2 cut(s) 180, 199
PkrI GCNGC 2 cut(s) 36, 381
PscI ACATGT 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 191, 210
PspPI GGNCC 1 cut(s) 189
PstI CTGCAG 2 cut(s) 384, 430
PstNI CAGNNNCTG 1 cut(s) 425
SatI GCNGC 2 cut(s) 35, 380
Sau96I GGNCC 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 269
SetI ASST 8 cut(s) 15, 36, 147, 171, 336, 381, 449, 455
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 380, 426
SinI GGWCC 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 2 cut(s) 76, 464
SsiI CCGC 2 cut(s) 4, 242
SspMI CTAG 3 cut(s) 281, 402, 450
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 99
TaqI TCGA 2 cut(s) 183, 246
TasI AATT 2 cut(s) 76, 464
TfiI GAWTC 2 cut(s) 180, 199
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 2 cut(s) 34, 379
Tsp45I GTSAC 1 cut(s) 409
TspRI CASTG 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 1 cut(s) 76
XbaI TCTAGA 1 cut(s) 280
XceI RCATGY 1 cut(s) 390
XspI CTAG 3 cut(s) 281, 402, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.