Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004191.1

Orthogroup ID

OG0010182

Physical Location & Seq

Genomic Coordinates Reverse (-)

236438 .. 237523

Gene Structure 1086 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0004191.1_g000055.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

atgtccaccaccgcaacgagctcagaatccacggcggagctaatcccctccctcccatacgacgtcgcttcgaactgcctagctcggatcccacgtcactaccaccctgtcctctcaatcctatccaaacctatccgctctctcctctcctcctctttcttcttcaccacccgctccctcctcaactccgcccaacacctcctctacctcaccctccgctctcccgaccactgcgccacctggttcgccctctaccaaaaccccaacccccgaaacgacgccgtccgcctcctcgtccccatccctcccgtccccgtccccgctgtcggcgccgcctatgccgttgtcggcccggagatctacgtcctcggcgggtccgtcaacgacgcgccgtccaataacgtctgggtcctcgactgccgcttccacacctggcgcgccgggccgccgatgcgcgtggcgcgtgagttcgcagcggctggggttgtggacggcagaattttcgtgatcggtggatgcgtgacggactcttggacgaggtcgaaatactgggccgaggctctcgacccgggttcgggtgtgtgggaggccgtgccgagcccggttgaggttcggggcaagtggatgcacgcgagtgcggtgataggtgggcgggtttatgcgctggcagaccggggcggggtggtttatgacccgagaacccgggtttgggagggggtggagaagaggatggacatggggtggagagggagggcttgcgtggtgcatgggatgttgtattgttatgattatttggggaagattagagggtttgacgcaaaaaatcgaatgtggaaggagctcaagggagtggagaagaagctgccgaagttcttgtgcggcgcgacaatggcgaatgtgggggagaatttggttgttctgtgggaaaccaagggaaatgggaaagatatggagatttggtgtgcggagattgaggtgaaggaaaatggagatggggagttgtgggggagaatcgattggtcccagaagcttctttcggttccaaagcactccaacattgtacaatgcttggcggttgatgtttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box Kelch_1

Protein Analysis

361

Amino Acids

40.0

Weight (kDa)

8.66

Isoelectric Point (pI)

38.68

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77880 AT1G77880 AT1G77880 AT2G21950
fragaria_vesca	FvH4_1g22291 FvH4_1g22291
prunus_persica	Prupe.6G172900_v2.0.a1 Prupe.6G172900_v2.0.a1
pyrus_communis	pycom15g29250
rosa_chinensis	RchiOBHm_Chr2g0116481
rosa_laevigata	RLG00000018275 RLG00000018277
rosa_multiflora	Rmu_sc0004191.1_g000055 Rmu_sc0019528.1_g000002
rosa_roxburghii	Rroxscaffold_2G00127160 Rroxscaffold_2G00127170
rosa_rugosa	Rorug02G0206800 Rorug02G0206900
rosa_samantha	Rh2CG268600 Rh2DG288700
rosa_wichuraiana	Rw2G020610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	66
AccB1I	GGYRCC	1 cut(s)	329
AccBSI	CCGCTC	3 cut(s)	138, 174, 219
AccII	CGCG	6 cut(s)	389, 438, 456, 463, 632, 884
AclWI	GGATC	2 cut(s)	82, 95
AcsI	RAATTY	2 cut(s)	498, 907
AcyI	GRCGYC	3 cut(s)	63, 279, 330
AfaI	GTAC	1 cut(s)	1062
AfiI	CCNNNNNNNGG	7 cut(s)	326, 574, 575, 607, 679, 708, 709
AhdI	GACNNNNNGTC	1 cut(s)	391
AjiI	CACGTC	1 cut(s)	95
AjnI	CCWGG	2 cut(s)	239, 431
AjuI	GAANNNNNNNTTGG	2 cut(s)	691, 723
AleI	CACNNNNGTG	1 cut(s)	633
AluBI	AGCT	6 cut(s)	21, 40, 83, 841, 862, 1030
AluI	AGCT	6 cut(s)	21, 40, 83, 841, 862, 1030
Alw21I	GWGCWC	2 cut(s)	23, 843
AlwI	GGATC	2 cut(s)	82, 95
AlwNI	CAGNNNCTG	1 cut(s)	479
Ama87I	CYCGRG	3 cut(s)	568, 694, 702
AoxI	GGCC	4 cut(s)	349, 443, 552, 588
ApeKI	GCWGC	2 cut(s)	473, 862
ApoI	RAATTY	2 cut(s)	498, 907
AscI	GGCGCGCC	1 cut(s)	436
AspLEI	GCGC	9 cut(s)	236, 332, 391, 438, 440, 456, 463, 664, 884
AspS9I	GGNCC	6 cut(s)	350, 375, 409, 443, 552, 1020
AsuC2I	CCSGG	8 cut(s)	353, 442, 569, 570, 602, 674, 703, 704
AsuHPI	GGTGA	4 cut(s)	157, 202, 652, 988
AsuII	TTCGAA	1 cut(s)	71
AvaI	CYCGRG	3 cut(s)	568, 694, 702
AvaII	GGWCC	3 cut(s)	375, 409, 1020
BamHI	GGATCC	1 cut(s)	87
BanI	GGYRCC	1 cut(s)	329
BanII	GRGCYC	3 cut(s)	23, 602, 843
Bbv12I	GWGCWC	2 cut(s)	23, 843
BbvI	GCAGC	2 cut(s)	485, 849
BccI	CCATC	3 cut(s)	308, 724, 986
BceAI	ACGGC	6 cut(s)	48, 266, 326, 376, 508, 575
BcgI	CGANNNNNNTGC	2 cut(s)	484, 518
BciT130I	CCWGG	2 cut(s)	241, 433
BcnI	CCSGG	8 cut(s)	353, 442, 569, 570, 602, 674, 703, 704
BfaI	CTAG	1 cut(s)	80
BfoI	RGCGCY	1 cut(s)	333
BglII	AGATCT	1 cut(s)	357
BisI	GCNGC	7 cut(s)	333, 421, 446, 474, 477, 863, 880
BlsI	GCNGC	7 cut(s)	334, 422, 447, 475, 478, 864, 881
Bme18I	GGWCC	3 cut(s)	375, 409, 1020
BmeRI	GACNNNNNGTC	1 cut(s)	391
BmeT110I	CYCGRG	3 cut(s)	568, 694, 702
BmgBI	CACGTC	1 cut(s)	95
BmgT120I	GGNCC	6 cut(s)	350, 375, 409, 443, 552, 1020
BmiI	GGNNCC	6 cut(s)	89, 331, 376, 410, 1022, 1041
BmrI	ACTGGG	1 cut(s)	559
BmsI	GCATC	3 cut(s)	441, 506, 615
BmuI	ACTGGG	1 cut(s)	559
Bpu14I	TTCGAA	1 cut(s)	71
BpuEI	CTTGAG	1 cut(s)	827
BpuMI	CCSGG	8 cut(s)	353, 442, 569, 570, 602, 674, 703, 704
Bsa29I	ATCGAT	1 cut(s)	1014
BsaHI	GRCGYC	3 cut(s)	63, 279, 330
BsaJI	CCNNGG	7 cut(s)	30, 367, 555, 568, 673, 702, 930
BsaXI	ACNNNNNCTCC	2 cut(s)	944, 974
Bsc4I	CCNNNNNNNGG	7 cut(s)	326, 574, 575, 607, 679, 708, 709
Bse1I	ACTGG	1 cut(s)	554
BseBI	CCWGG	2 cut(s)	241, 433
BseCI	ATCGAT	1 cut(s)	1014
BseDI	CCNNGG	7 cut(s)	30, 367, 555, 568, 673, 702, 930
BseGI	GGATG	5 cut(s)	300, 521, 630, 735, 777
BseLI	CCNNNNNNNGG	7 cut(s)	326, 574, 575, 607, 679, 708, 709
BseMII	CTCAG	1 cut(s)	36
BseNI	ACTGG	1 cut(s)	554
BsePI	GCGCGC	1 cut(s)	436
BseRI	GAGGAG	6 cut(s)	134, 139, 142, 170, 191, 281
BseXI	GCAGC	2 cut(s)	485, 849
BseYI	CCCAGC	1 cut(s)	479
Bsh1236I	CGCG	6 cut(s)	389, 438, 456, 463, 632, 884
BshFI	GGCC	4 cut(s)	351, 445, 554, 590
BshNI	GGYRCC	1 cut(s)	329
BshVI	ATCGAT	1 cut(s)	1014
BsiHKAI	GWGCWC	2 cut(s)	23, 843
BsiHKCI	CYCGRG	3 cut(s)	568, 694, 702
BsiSI	CCGG	6 cut(s)	353, 441, 569, 602, 673, 703
BslFI	GGGAC	4 cut(s)	281, 296, 302, 1006
BslI	CCNNNNNNNGG	7 cut(s)	326, 574, 575, 607, 679, 708, 709
BsmFI	GGGAC	4 cut(s)	281, 296, 302, 1006
BsnI	GGCC	4 cut(s)	351, 445, 554, 590
BsoBI	CYCGRG	3 cut(s)	568, 694, 702
Bsp119I	TTCGAA	1 cut(s)	71
Bsp1286I	GDGCHC	3 cut(s)	23, 602, 843
Bsp1407I	TGTACA	1 cut(s)	1060
Bsp143I	GATC	3 cut(s)	87, 357, 507
BspANI	GGCC	4 cut(s)	351, 445, 554, 590
BspCNI	CTCAG	1 cut(s)	35
BspDI	ATCGAT	1 cut(s)	1014
BspFNI	CGCG	6 cut(s)	389, 438, 456, 463, 632, 884
BspLI	GGNNCC	6 cut(s)	89, 331, 376, 410, 1022, 1041
BspPI	GGATC	2 cut(s)	82, 95
BspT104I	TTCGAA	1 cut(s)	71
BspT107I	GGYRCC	1 cut(s)	329
BsrBI	CCGCTC	3 cut(s)	138, 174, 219
BsrGI	TGTACA	1 cut(s)	1060
BsrI	ACTGG	1 cut(s)	554
BssECI	CCNNGG	7 cut(s)	30, 367, 555, 568, 673, 702, 930
BssHII	GCGCGC	1 cut(s)	436
BssMI	GATC	3 cut(s)	87, 357, 507
BssNI	GRCGYC	3 cut(s)	63, 279, 330
BssT1I	CCWWGG	1 cut(s)	930
Bst2UI	CCWGG	2 cut(s)	241, 433
Bst6I	CTCTTC	1 cut(s)	719
BstACI	GRCGYC	3 cut(s)	63, 279, 330
BstAUI	TGTACA	1 cut(s)	1060
BstBI	TTCGAA	1 cut(s)	71
BstC8I	GCNNGC	4 cut(s)	438, 630, 666, 757
BstDEI	CTNAG	1 cut(s)	22
BstDSI	CCRYGG	1 cut(s)	30
BstF5I	GGATG	5 cut(s)	300, 521, 630, 735, 777
BstFNI	CGCG	6 cut(s)	389, 438, 456, 463, 632, 884
BstH2I	RGCGCY	1 cut(s)	333
BstHHI	GCGC	9 cut(s)	236, 332, 391, 438, 440, 456, 463, 664, 884
BstKTI	GATC	3 cut(s)	90, 360, 510
BstMBI	GATC	3 cut(s)	87, 357, 507
BstMWI	GCNNNNNNNGC	6 cut(s)	329, 338, 442, 451, 460, 890
BstNI	CCWGG	2 cut(s)	241, 433
BstUI	CGCG	6 cut(s)	389, 438, 456, 463, 632, 884
BstV1I	GCAGC	2 cut(s)	485, 849
BstX2I	RGATCY	2 cut(s)	87, 357
BstYI	RGATCY	2 cut(s)	87, 357
Bsu15I	ATCGAT	1 cut(s)	1014
BsuRI	GGCC	4 cut(s)	351, 445, 554, 590
BsuTUI	ATCGAT	1 cut(s)	1014
BtgI	CCRYGG	1 cut(s)	30
BtrI	CACGTC	1 cut(s)	95
BtsCI	GGATG	5 cut(s)	300, 521, 630, 735, 777
BtsI	GCAGTG	1 cut(s)	229
BtsIMutI	CAGTG	1 cut(s)	229
Cac8I	GCNNGC	4 cut(s)	438, 630, 666, 757
CaiI	CAGNNNCTG	1 cut(s)	479
CfoI	GCGC	9 cut(s)	236, 332, 391, 438, 440, 456, 463, 664, 884
Cfr13I	GGNCC	6 cut(s)	350, 375, 409, 443, 552, 1020
Cfr9I	CCCGGG	2 cut(s)	568, 702
ClaI	ATCGAT	1 cut(s)	1014
CseI	GACGC	3 cut(s)	287, 395, 824
CsiI	ACCWGGT	1 cut(s)	239
Csp6I	GTAC	1 cut(s)	1061
CviAII	CATG	2 cut(s)	736, 767
CviQI	GTAC	1 cut(s)	1061
DdeI	CTNAG	1 cut(s)	22
DinI	GGCGCC	1 cut(s)	331
DpnI	GATC	3 cut(s)	89, 359, 509
DpnII	GATC	3 cut(s)	87, 357, 507
DriI	GACNNNNNGTC	1 cut(s)	391
Eam1104I	CTCTTC	1 cut(s)	719
Eam1105I	GACNNNNNGTC	1 cut(s)	391
EarI	CTCTTC	1 cut(s)	719
EciI	GGCGGA	3 cut(s)	50, 178, 275
Ecl136II	GAGCTC	2 cut(s)	21, 841
Eco130I	CCWWGG	1 cut(s)	930
Eco24I	GRGCYC	3 cut(s)	23, 602, 843
Eco47I	GGWCC	3 cut(s)	375, 409, 1020
Eco53kI	GAGCTC	2 cut(s)	21, 841
Eco88I	CYCGRG	3 cut(s)	568, 694, 702
EcoICRI	GAGCTC	2 cut(s)	21, 841
EcoO109I	RGGNCCY	1 cut(s)	409
EcoRII	CCWGG	2 cut(s)	239, 431
EcoT14I	CCWWGG	1 cut(s)	930
EcoT38I	GRGCYC	3 cut(s)	23, 602, 843
EgeI	GGCGCC	1 cut(s)	331
EheI	GGCGCC	1 cut(s)	331
ErhI	CCWWGG	1 cut(s)	930
FaeI	CATG	2 cut(s)	739, 770
FaiI	YATR	8 cut(s)	58, 339, 660, 690, 737, 768, 786, 950
FaqI	GGGAC	4 cut(s)	281, 296, 302, 1006
FatI	CATG	2 cut(s)	735, 766
FauI	CCCGC	5 cut(s)	179, 328, 365, 645, 671
Fnu4HI	GCNGC	7 cut(s)	333, 421, 446, 474, 477, 863, 880
FokI	GGATG	5 cut(s)	287, 528, 637, 742, 784
FriOI	GRGCYC	3 cut(s)	23, 602, 843
Fsp4HI	GCNGC	7 cut(s)	333, 421, 446, 474, 477, 863, 880
FspBI	CTAG	1 cut(s)	80
GlaI	GCGC	9 cut(s)	235, 331, 390, 437, 439, 455, 462, 663, 883
GluI	GCNGC	7 cut(s)	333, 421, 446, 474, 477, 863, 880
GsaI	CCCAGC	1 cut(s)	483
HaeII	RGCGCY	1 cut(s)	333
HaeIII	GGCC	4 cut(s)	351, 445, 554, 590
HapII	CCGG	6 cut(s)	353, 441, 569, 602, 673, 703
HgaI	GACGC	3 cut(s)	287, 395, 824
HhaI	GCGC	9 cut(s)	236, 332, 391, 438, 440, 456, 463, 664, 884
Hin1I	GRCGYC	3 cut(s)	63, 279, 330
Hin1II	CATG	2 cut(s)	739, 770
Hin6I	GCGC	9 cut(s)	234, 330, 389, 436, 438, 454, 461, 662, 882
HinP1I	GCGC	9 cut(s)	234, 330, 389, 436, 438, 454, 461, 662, 882
HincII	GTYRAC	1 cut(s)	382
HindII	GTYRAC	1 cut(s)	382
HindIII	AAGCTT	1 cut(s)	1028
HinfI	GANTC	3 cut(s)	26, 527, 1011
HpaII	CCGG	6 cut(s)	353, 441, 569, 602, 673, 703
HphI	GGTGA	4 cut(s)	157, 202, 652, 988
Hpy166II	GTNNAC	3 cut(s)	6, 382, 490
Hpy188I	TCNGA	2 cut(s)	25, 87
Hpy188III	TCNNGA	3 cut(s)	224, 505, 563
Hpy8I	GTNNAC	3 cut(s)	6, 382, 490
Hpy99I	CGWCG	4 cut(s)	65, 68, 281, 389
HpyAV	CCTTC	2 cut(s)	829, 973
HpyCH4IV	ACGT	4 cut(s)	63, 94, 363, 402
HpyCH4V	TGCA	2 cut(s)	628, 766
HpyF10VI	GCNNNNNNNGC	6 cut(s)	329, 338, 442, 451, 460, 890
HpyF3I	CTNAG	1 cut(s)	22
HpySE526I	ACGT	4 cut(s)	63, 94, 363, 402
Hsp92I	GRCGYC	3 cut(s)	63, 279, 330
Hsp92II	CATG	2 cut(s)	739, 770
HspAI	GCGC	9 cut(s)	234, 330, 389, 436, 438, 454, 461, 662, 882
KasI	GGCGCC	1 cut(s)	329
Kzo9I	GATC	3 cut(s)	87, 357, 507
LmnI	GCTCC	3 cut(s)	37, 179, 838
Lsp1109I	GCAGC	2 cut(s)	485, 849
LweI	GCATC	3 cut(s)	441, 506, 615
MabI	ACCWGGT	1 cut(s)	239
MaeI	CTAG	1 cut(s)	80
MaeII	ACGT	4 cut(s)	63, 94, 363, 402
MaeIII	GTNAC	2 cut(s)	95, 520
MalI	GATC	3 cut(s)	89, 359, 509
MbiI	CCGCTC	3 cut(s)	138, 174, 219
MboI	GATC	3 cut(s)	87, 357, 507
MboII	GAAGA	5 cut(s)	151, 154, 736, 811, 868
MflI	RGATCY	2 cut(s)	87, 357
MhlI	GDGCHC	3 cut(s)	23, 602, 843
MluCI	AATT	2 cut(s)	498, 907
Mly113I	GGCGCC	1 cut(s)	330
MlyI	GAGTC	1 cut(s)	521
MmeI	TCCRAC	1 cut(s)	1077
MslI	CAYNNNNRTG	1 cut(s)	633
MspA1I	CMGCKG	2 cut(s)	323, 476
MspI	CCGG	6 cut(s)	353, 441, 569, 602, 673, 703
MvaI	CCWGG	2 cut(s)	241, 433
MvnI	CGCG	6 cut(s)	389, 438, 456, 463, 632, 884
MwoI	GCNNNNNNNGC	6 cut(s)	329, 338, 442, 451, 460, 890
NarI	GGCGCC	1 cut(s)	330
NciI	CCSGG	8 cut(s)	353, 442, 569, 570, 602, 674, 703, 704
NdeII	GATC	3 cut(s)	87, 357, 507
NlaIII	CATG	2 cut(s)	739, 770
NlaIV	GGNNCC	6 cut(s)	89, 331, 376, 410, 1022, 1041
NmeAIII	GCCGAG	3 cut(s)	348, 580, 621
NmuCI	GTSAC	2 cut(s)	95, 520
NspV	TTCGAA	1 cut(s)	71
OliI	CACNNNNGTG	1 cut(s)	633
PalAI	GGCGCGCC	1 cut(s)	436
PauI	GCGCGC	1 cut(s)	436
PcsI	WCGNNNNNNNCGW	3 cut(s)	91, 375, 516
PfeI	GAWTC	2 cut(s)	26, 1011
PflFI	GACNNNGTC	2 cut(s)	281, 538
PkrI	GCNGC	7 cut(s)	334, 422, 447, 475, 478, 864, 881
PleI	GAGTC	1 cut(s)	521
PluTI	GGCGCC	1 cut(s)	333
PpsI	GAGTC	1 cut(s)	521
PpuMI	RGGWCCY	1 cut(s)	409
Psp124BI	GAGCTC	2 cut(s)	23, 843
Psp5II	RGGWCCY	1 cut(s)	409
Psp6I	CCWGG	2 cut(s)	239, 431
PspFI	CCCAGC	1 cut(s)	479
PspGI	CCWGG	2 cut(s)	239, 431
PspN4I	GGNNCC	6 cut(s)	89, 331, 376, 410, 1022, 1041
PspPI	GGNCC	6 cut(s)	350, 375, 409, 443, 552, 1020
PspPPI	RGGWCCY	1 cut(s)	409
PstNI	CAGNNNCTG	1 cut(s)	479
PsuI	RGATCY	2 cut(s)	87, 357
PsyI	GACNNNGTC	2 cut(s)	281, 538
PteI	GCGCGC	1 cut(s)	436
RsaI	GTAC	1 cut(s)	1062
RsaNI	GTAC	1 cut(s)	1061
RseI	CAYNNNNRTG	1 cut(s)	633
SacI	GAGCTC	2 cut(s)	23, 843
SatI	GCNGC	7 cut(s)	333, 421, 446, 474, 477, 863, 880
Sau3AI	GATC	3 cut(s)	87, 357, 507
Sau96I	GGNCC	6 cut(s)	350, 375, 409, 443, 552, 1020
SchI	GAGTC	1 cut(s)	521
SduI	GDGCHC	3 cut(s)	23, 602, 843
SexAI	ACCWGGT	1 cut(s)	239
SfaNI	GCATC	3 cut(s)	441, 506, 615
SfoI	GGCGCC	1 cut(s)	331
SfuI	TTCGAA	1 cut(s)	71
SgsI	GGCGCGCC	1 cut(s)	436
SinI	GGWCC	3 cut(s)	375, 409, 1020
SmaI	CCCGGG	2 cut(s)	570, 704
SmiMI	CAYNNNNRTG	1 cut(s)	633
SmlI	CTYRAG	1 cut(s)	842
SmoI	CTYRAG	1 cut(s)	842
Sse9I	AATT	2 cut(s)	498, 907
SspDI	GGCGCC	1 cut(s)	329
SspMI	CTAG	1 cut(s)	80
SstI	GAGCTC	2 cut(s)	23, 843
StyI	CCWWGG	1 cut(s)	930
TaiI	ACGT	4 cut(s)	66, 97, 366, 405
TaqI	TCGA	6 cut(s)	71, 414, 542, 564, 826, 1014
TasI	AATT	2 cut(s)	498, 907
TatI	WGTACW	1 cut(s)	1060
TauI	GCSGC	5 cut(s)	335, 423, 448, 479, 882
TfiI	GAWTC	2 cut(s)	26, 1011
TscAI	CASTG	1 cut(s)	236
TseFI	GTSAC	2 cut(s)	95, 520
TseI	GCWGC	2 cut(s)	473, 862
Tsp45I	GTSAC	2 cut(s)	95, 520
TspGWI	ACGGA	2 cut(s)	367, 539
TspMI	CCCGGG	2 cut(s)	568, 702
TspRI	CASTG	1 cut(s)	236
Tth111I	GACNNNGTC	2 cut(s)	281, 538
VpaK11BI	GGWCC	3 cut(s)	375, 409, 1020
XapI	RAATTY	2 cut(s)	498, 907
XmaI	CCCGGG	2 cut(s)	568, 702
XspI	CTAG	1 cut(s)	80
ZraI	GACGTC	1 cut(s)	64

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.