RLG00000018275

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010182
Physical Location & Seq
Forward (+)
27823078 .. 27824163
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018275

Sequence Viewer

Length: 1086 bp
ATGTCCACCACCGCAACGAACTCAGAATCCACGGCGGAGCTAATCCCCTCCCTCCCATACGACGTCGCTTCGAACTGCCTAGCTCGAATCCCACGCCACTACCACCCTGTCCTCTCAATCCTATCCAAACCCATCCGCTCTCTCCTCTCCTCCCCTTTCTTCTTCACCATCCGCTCCGTCCTCAACTCCGCCCAACACCTCCTCTACCTCACCCTCCGCTCTCCCCACCACCGCGCCACCTGGTTCGCCCTCTACCAAAACCCCAACCCCCGAAACGACGCCGTCCGCCTCCTCGTCCCCATCCCGCCCGTCCCCGTCCTCGCCGTCGGAGCCGCCTACGCCGTCGTCGGCCCGGAGATCTACGTCCTCGGCGGGTCCGTGAACGACGCGCCGTCCAATAACGTCTGGGTCCTCGACTGCCGCTTCCACACCTGGCGCGCCGGGCCGGCGATGCGCGTGGCGCGTGAGTTCGCAGCGGCTGGGGTTGTGGACGGGAGAATTTACGTGATCGGTGGATGCGTGACGGACTCGTGGACGAGGTCGGAATACTGGGCCGAGGCTCTCGAACCGGGTTCGGGTGTTTGGGAGGCCGTGCCGAGCCCGGTTGAGGTTCGGGGCAAGTGGATGCACGCGAGTGCGGTGATCGGCGGGCGGGTTTATGCGCTGGCGGACCGGGGCGGGGTGGTTTATGACCCGAGAACCCGGGTTTGGGAGGGGGTGGAGAAGAGGATGGACATGGGGTGGAGAGGGAGGGCTTGCGTGGTGCATGGGATGTTGTATTGTTATGATTATTTGGGGAAGATTAGAGGGTTTGACGCGAAAAATCGAATGTGGAAGGAGCTCAAGGGAGTGGAGAAGAAGCTGCCGAAGTTCTTGTGCGGCGCGACAATGGCGAATGTGGGGGAGAATTTGGTTGTTCTGTGGGAAACCAAGGGAAATGGGAAAGATATGGAGATTTGGTGTGCGGAGATTGAGGTGAAGGAAAATGGAGATGGGGAGTTGTGGGGGAGAATCGATTGGTCCCAGAAGCTTCTTTCGGTTCCAAAGCACTCCAACATTGTACAATGCTTGGCGGTTGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

362

Amino Acids

40.13

Weight (kDa)

8.7

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 81 - 344 6.5e-82 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 101 - 178 1e-05 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 105 - 243 1e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 108 - 185 8.3e-07 FBX42, beta-propeller domain
Kelch_1 PF01344 110 - 151 2.7e-10 Kelch motif
Kelch_1 PF01344 155 - 200 7.1e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccBSI CCGCTC 3 cut(s) 138, 174, 219
AccII CGCG 8 cut(s) 234, 389, 438, 456, 463, 632, 818, 884
AcsI RAATTY 2 cut(s) 498, 907
AcyI GRCGYC 2 cut(s) 63, 279
AfaI GTAC 1 cut(s) 1062
AfiI CCNNNNNNNGG 5 cut(s) 575, 607, 679, 708, 709
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 2 cut(s) 239, 431
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
AleI CACNNNNGTG 1 cut(s) 633
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 5 cut(s) 40, 83, 841, 862, 1030
AluI AGCT 5 cut(s) 40, 83, 841, 862, 1030
Alw21I GWGCWC 1 cut(s) 843
AlwNI CAGNNNCTG 1 cut(s) 479
Ama87I CYCGRG 2 cut(s) 694, 702
AoxI GGCC 4 cut(s) 349, 443, 552, 588
ApeKI GCWGC 2 cut(s) 473, 862
ApoI RAATTY 2 cut(s) 498, 907
AscI GGCGCGCC 1 cut(s) 436
AspLEI GCGC 8 cut(s) 236, 391, 438, 440, 456, 463, 664, 884
AspS9I GGNCC 7 cut(s) 350, 375, 409, 443, 552, 670, 1020
AsuC2I CCSGG 7 cut(s) 353, 442, 570, 602, 674, 703, 704
AsuHPI GGTGA 4 cut(s) 157, 202, 652, 988
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 2 cut(s) 694, 702
AvaII GGWCC 4 cut(s) 375, 409, 670, 1020
BanII GRGCYC 2 cut(s) 602, 843
BauI CACGAG 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 2 cut(s) 485, 849
BccI CCATC 5 cut(s) 140, 176, 308, 724, 986
BceAI ACGGC 6 cut(s) 48, 266, 308, 326, 376, 575
BciT130I CCWGG 2 cut(s) 241, 433
BcnI CCSGG 7 cut(s) 353, 442, 570, 602, 674, 703, 704
BfaI CTAG 1 cut(s) 80
BglI GCCNNNNNGGC 1 cut(s) 446
BglII AGATCT 1 cut(s) 357
BisI GCNGC 6 cut(s) 333, 421, 474, 477, 863, 880
BlsI GCNGC 6 cut(s) 334, 422, 475, 478, 864, 881
Bme1390I CCNGG 9 cut(s) 241, 353, 433, 442, 570, 602, 674, 703, 704
Bme18I GGWCC 4 cut(s) 375, 409, 670, 1020
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 2 cut(s) 694, 702
BmgT120I GGNCC 7 cut(s) 350, 375, 409, 443, 552, 670, 1020
BmiI GGNNCC 5 cut(s) 331, 376, 410, 1022, 1041
BmrFI CCNGG 9 cut(s) 241, 353, 433, 442, 570, 602, 674, 703, 704
BmrI ACTGGG 1 cut(s) 559
BmsI GCATC 3 cut(s) 441, 506, 615
BmuI ACTGGG 1 cut(s) 559
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 827
BpuMI CCSGG 7 cut(s) 353, 442, 570, 602, 674, 703, 704
Bsa29I ATCGAT 1 cut(s) 1014
BsaAI YACGTR 1 cut(s) 505
BsaHI GRCGYC 2 cut(s) 63, 279
BsaJI CCNNGG 6 cut(s) 30, 367, 555, 673, 702, 930
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 5 cut(s) 575, 607, 679, 708, 709
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 241, 433
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 6 cut(s) 30, 367, 555, 673, 702, 930
BseGI GGATG 7 cut(s) 132, 168, 300, 521, 630, 735, 777
BseLI CCNNNNNNNGG 5 cut(s) 575, 607, 679, 708, 709
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 554
BsePI GCGCGC 1 cut(s) 436
BseRI GAGGAG 4 cut(s) 134, 139, 191, 281
BseXI GCAGC 2 cut(s) 485, 849
BseYI CCCAGC 1 cut(s) 479
Bsh1236I CGCG 8 cut(s) 234, 389, 438, 456, 463, 632, 818, 884
BshFI GGCC 4 cut(s) 351, 445, 554, 590
BshVI ATCGAT 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 2 cut(s) 694, 702
BsiSI CCGG 7 cut(s) 353, 441, 446, 569, 602, 673, 703
BslFI GGGAC 3 cut(s) 281, 296, 1006
BslI CCNNNNNNNGG 5 cut(s) 575, 607, 679, 708, 709
BsmFI GGGAC 3 cut(s) 281, 296, 1006
BsnI GGCC 4 cut(s) 351, 445, 554, 590
BsoBI CYCGRG 2 cut(s) 694, 702
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 602, 843
Bsp1407I TGTACA 1 cut(s) 1060
Bsp143I GATC 3 cut(s) 357, 507, 642
BspANI GGCC 4 cut(s) 351, 445, 554, 590
BspCNI CTCAG 1 cut(s) 35
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 8 cut(s) 234, 389, 438, 456, 463, 632, 818, 884
BspLI GGNNCC 5 cut(s) 331, 376, 410, 1022, 1041
BspT104I TTCGAA 1 cut(s) 71
BsrBI CCGCTC 3 cut(s) 138, 174, 219
BsrFI RCCGGY 1 cut(s) 445
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 1 cut(s) 554
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 6 cut(s) 30, 367, 555, 673, 702, 930
BssHII GCGCGC 1 cut(s) 436
BssMI GATC 3 cut(s) 357, 507, 642
BssNI GRCGYC 2 cut(s) 63, 279
BssSI CACGAG 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 930
Bst2BI CACGAG 1 cut(s) 529
Bst2UI CCWGG 2 cut(s) 241, 433
Bst6I CTCTTC 1 cut(s) 719
BstACI GRCGYC 2 cut(s) 63, 279
BstAUI TGTACA 1 cut(s) 1060
BstBAI YACGTR 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 6 cut(s) 438, 447, 630, 650, 666, 757
BstDEI CTNAG 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 7 cut(s) 132, 168, 300, 521, 630, 735, 777
BstFNI CGCG 8 cut(s) 234, 389, 438, 456, 463, 632, 818, 884
BstHHI GCGC 8 cut(s) 236, 391, 438, 440, 456, 463, 664, 884
BstKTI GATC 3 cut(s) 360, 510, 645
BstMBI GATC 3 cut(s) 357, 507, 642
BstMWI GCNNNNNNNGC 7 cut(s) 329, 338, 442, 446, 451, 460, 890
BstNI CCWGG 2 cut(s) 241, 433
BstSCI CCNGG 9 cut(s) 239, 351, 431, 440, 568, 600, 672, 701, 702
BstUI CGCG 8 cut(s) 234, 389, 438, 456, 463, 632, 818, 884
BstV1I GCAGC 2 cut(s) 485, 849
BstX2I RGATCY 1 cut(s) 357
BstYI RGATCY 1 cut(s) 357
Bsu15I ATCGAT 1 cut(s) 1014
BsuRI GGCC 4 cut(s) 351, 445, 554, 590
BsuTUI ATCGAT 1 cut(s) 1014
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 7 cut(s) 132, 168, 300, 521, 630, 735, 777
Cac8I GCNNGC 6 cut(s) 438, 447, 630, 650, 666, 757
CaiI CAGNNNCTG 1 cut(s) 479
CfoI GCGC 8 cut(s) 236, 391, 438, 440, 456, 463, 664, 884
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 7 cut(s) 350, 375, 409, 443, 552, 670, 1020
Cfr9I CCCGGG 1 cut(s) 702
ClaI ATCGAT 1 cut(s) 1014
CpoI CGGWCCG 1 cut(s) 670
CseI GACGC 3 cut(s) 287, 395, 824
CsiI ACCWGGT 1 cut(s) 239
Csp6I GTAC 1 cut(s) 1061
CspI CGGWCCG 1 cut(s) 670
CviAII CATG 2 cut(s) 736, 767
CviQI GTAC 1 cut(s) 1061
DdeI CTNAG 1 cut(s) 22
DpnI GATC 3 cut(s) 359, 509, 644
DpnII GATC 3 cut(s) 357, 507, 642
DriI GACNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 719
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 719
EciI GGCGGA 4 cut(s) 50, 178, 275, 683
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 1 cut(s) 930
Eco24I GRGCYC 2 cut(s) 602, 843
Eco47I GGWCC 4 cut(s) 375, 409, 670, 1020
Eco53kI GAGCTC 1 cut(s) 841
Eco88I CYCGRG 2 cut(s) 694, 702
EcoICRI GAGCTC 1 cut(s) 841
EcoO109I RGGNCCY 1 cut(s) 409
EcoRII CCWGG 2 cut(s) 239, 431
EcoT14I CCWWGG 1 cut(s) 930
EcoT38I GRGCYC 2 cut(s) 602, 843
ErhI CCWWGG 1 cut(s) 930
FaeI CATG 2 cut(s) 739, 770
FaiI YATR 7 cut(s) 58, 660, 690, 737, 768, 786, 950
FaqI GGGAC 3 cut(s) 281, 296, 1006
FatI CATG 2 cut(s) 735, 766
FauI CCCGC 5 cut(s) 312, 365, 641, 645, 671
Fnu4HI GCNGC 6 cut(s) 333, 421, 474, 477, 863, 880
FokI GGATG 7 cut(s) 119, 155, 287, 528, 637, 742, 784
FriOI GRGCYC 2 cut(s) 602, 843
Fsp4HI GCNGC 6 cut(s) 333, 421, 474, 477, 863, 880
FspBI CTAG 1 cut(s) 80
GlaI GCGC 8 cut(s) 235, 390, 437, 439, 455, 462, 663, 883
GluI GCNGC 6 cut(s) 333, 421, 474, 477, 863, 880
GsaI CCCAGC 1 cut(s) 483
HaeIII GGCC 4 cut(s) 351, 445, 554, 590
HapII CCGG 7 cut(s) 353, 441, 446, 569, 602, 673, 703
HgaI GACGC 3 cut(s) 287, 395, 824
HhaI GCGC 8 cut(s) 236, 391, 438, 440, 456, 463, 664, 884
Hin1I GRCGYC 2 cut(s) 63, 279
Hin1II CATG 2 cut(s) 739, 770
Hin6I GCGC 8 cut(s) 234, 389, 436, 438, 454, 461, 662, 882
HinP1I GCGC 8 cut(s) 234, 389, 436, 438, 454, 461, 662, 882
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 4 cut(s) 26, 87, 527, 1011
HpaII CCGG 7 cut(s) 353, 441, 446, 569, 602, 673, 703
HphI GGTGA 4 cut(s) 157, 202, 652, 988
Hpy166II GTNNAC 4 cut(s) 6, 382, 490, 534
Hpy188I TCNGA 3 cut(s) 25, 329, 544
Hpy188III TCNNGA 1 cut(s) 563
Hpy8I GTNNAC 4 cut(s) 6, 382, 490, 534
Hpy99I CGWCG 7 cut(s) 65, 68, 281, 329, 347, 350, 389
HpyAV CCTTC 2 cut(s) 829, 973
HpyCH4IV ACGT 4 cut(s) 63, 363, 402, 504
HpyCH4V TGCA 2 cut(s) 628, 766
HpyF10VI GCNNNNNNNGC 7 cut(s) 329, 338, 442, 446, 451, 460, 890
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 4 cut(s) 63, 363, 402, 504
Hsp92I GRCGYC 2 cut(s) 63, 279
Hsp92II CATG 2 cut(s) 739, 770
HspAI GCGC 8 cut(s) 234, 389, 436, 438, 454, 461, 662, 882
KroI GCCGGC 1 cut(s) 445
KroNI GCCGGC 1 cut(s) 447
Kzo9I GATC 3 cut(s) 357, 507, 642
LmnI GCTCC 4 cut(s) 37, 179, 329, 838
Lsp1109I GCAGC 2 cut(s) 485, 849
LweI GCATC 3 cut(s) 441, 506, 615
MabI ACCWGGT 1 cut(s) 239
MaeI CTAG 1 cut(s) 80
MaeII ACGT 4 cut(s) 63, 363, 402, 504
MaeIII GTNAC 1 cut(s) 520
MalI GATC 3 cut(s) 359, 509, 644
MbiI CCGCTC 3 cut(s) 138, 174, 219
MboI GATC 3 cut(s) 357, 507, 642
MboII GAAGA 5 cut(s) 151, 154, 736, 811, 868
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 2 cut(s) 602, 843
MluCI AATT 2 cut(s) 498, 907
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 3 cut(s) 307, 522, 1077
MroNI GCCGGC 1 cut(s) 445
MslI CAYNNNNRTG 1 cut(s) 633
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 7 cut(s) 353, 441, 446, 569, 602, 673, 703
MspR9I CCNGG 9 cut(s) 241, 353, 433, 442, 570, 602, 674, 703, 704
MvaI CCWGG 2 cut(s) 241, 433
MvnI CGCG 8 cut(s) 234, 389, 438, 456, 463, 632, 818, 884
MwoI GCNNNNNNNGC 7 cut(s) 329, 338, 442, 446, 451, 460, 890
NaeI GCCGGC 1 cut(s) 447
NciI CCSGG 7 cut(s) 353, 442, 570, 602, 674, 703, 704
NdeII GATC 3 cut(s) 357, 507, 642
NgoMIV GCCGGC 1 cut(s) 445
NlaIII CATG 2 cut(s) 739, 770
NlaIV GGNNCC 5 cut(s) 331, 376, 410, 1022, 1041
NmeAIII GCCGAG 3 cut(s) 348, 580, 621
NmuCI GTSAC 1 cut(s) 520
NspV TTCGAA 1 cut(s) 71
OliI CACNNNNGTG 1 cut(s) 633
PalAI GGCGCGCC 1 cut(s) 436
PauI GCGCGC 1 cut(s) 436
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 516
PdiI GCCGGC 1 cut(s) 447
PfeI GAWTC 3 cut(s) 26, 87, 1011
PflFI GACNNNGTC 2 cut(s) 281, 538
PkrI GCNGC 6 cut(s) 334, 422, 475, 478, 864, 881
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 239, 431
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 2 cut(s) 239, 431
PspN4I GGNNCC 5 cut(s) 331, 376, 410, 1022, 1041
PspPI GGNCC 7 cut(s) 350, 375, 409, 443, 552, 670, 1020
PspPPI RGGWCCY 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 357
PsyI GACNNNGTC 2 cut(s) 281, 538
PteI GCGCGC 1 cut(s) 436
RsaI GTAC 1 cut(s) 1062
RsaNI GTAC 1 cut(s) 1061
RseI CAYNNNNRTG 1 cut(s) 633
Rsr2I CGGWCCG 1 cut(s) 670
RsrII CGGWCCG 1 cut(s) 670
SacI GAGCTC 1 cut(s) 843
SatI GCNGC 6 cut(s) 333, 421, 474, 477, 863, 880
Sau3AI GATC 3 cut(s) 357, 507, 642
Sau96I GGNCC 7 cut(s) 350, 375, 409, 443, 552, 670, 1020
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 9 cut(s) 241, 353, 433, 442, 570, 602, 674, 703, 704
SduI GDGCHC 2 cut(s) 602, 843
SexAI ACCWGGT 1 cut(s) 239
SfaNI GCATC 3 cut(s) 441, 506, 615
SfuI TTCGAA 1 cut(s) 71
SgsI GGCGCGCC 1 cut(s) 436
SinI GGWCC 4 cut(s) 375, 409, 670, 1020
SmaI CCCGGG 1 cut(s) 704
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
Sse9I AATT 2 cut(s) 498, 907
SspMI CTAG 1 cut(s) 80
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 9 cut(s) 239, 351, 431, 440, 568, 600, 672, 701, 702
StyI CCWWGG 1 cut(s) 930
TaiI ACGT 4 cut(s) 66, 366, 405, 507
TaqI TCGA 6 cut(s) 71, 85, 414, 564, 826, 1014
TasI AATT 2 cut(s) 498, 907
TatI WGTACW 1 cut(s) 1060
TauI GCSGC 4 cut(s) 335, 423, 479, 882
TfiI GAWTC 3 cut(s) 26, 87, 1011
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 2 cut(s) 473, 862
Tsp45I GTSAC 1 cut(s) 520
TspGWI ACGGA 3 cut(s) 166, 367, 539
TspMI CCCGGG 1 cut(s) 702
Tth111I GACNNNGTC 2 cut(s) 281, 538
VpaK11BI GGWCC 4 cut(s) 375, 409, 670, 1020
XapI RAATTY 2 cut(s) 498, 907
XmaI CCCGGG 1 cut(s) 702
XspI CTAG 1 cut(s) 80
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.