Rw2G020610

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010182
Physical Location & Seq
Forward (+)
28126440 .. 28128114
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020610.1

Sequence Viewer

Length: 1086 bp
ATGTCCACCACCGCAACGAGCTCAGAATTCACGGCGGAGCTAATCCCCTCCCTCCCATACGACGTCGCTTCGAACTGCCTAGCTCGGATCCCACGTCACTACCACCCTATCCTCTCAATCCTATCCAAGCCCATCCGCTCTCTCCTCTCCTCCCCTTTCTTCTTCACCACCCGCTCCCTCCTCAACTCCGCCCAACACCTCCTCTACCTCACCCTCCGCTCTCCCGACCACCGCGCCACCTGGTTCGCCCTCTACCAAAACCCCAACCCCCGAAACGACGCCGTCCGCCTCCTCGTCCCCATCCCGCCCGTCCCCGTCCCGGCCGTCGGCGCCGCCTATGCCGTCGTCGGCCCGGAGATCTACGTCCTCGGCGGGTCCGTCAACGACGCGCCGTCCAATAACGTCTGGGTCCTCGACTGCCGCTTCCACACCTGGCACGCCGGGCCGCCGATGCGCGTGGCGCGTGAGTTCGCAGCGGCTGGGGTTGTGGACGGCAGAATTTTCGTGATCGGTGGATGCGTGACGGACTCTTGGACCAGGTCGAAATACTGGGCCGAGGCTCTCGACCCGGGTTCGGGTGTGTGGGAGGCCGTGCCGAGCCCGGTTGAGGTTCGGGGCAAGTGGATGCACGCGAGTGCGGTGATAGGTGGGCGGGTTTATGCGCTGGCGGACCGGGGCGGGGTGGTTTATGACCCGAGAACCCGGGTTTGGGAGGGGGTGGAGAAGAGGATGGACATGGGGTGGAGAGGGAGGGCTTGCGTGGTGCATGGGATGTTGTATTGTTATGATTATTTGGGGAAGATTAGAGGGTTTGACGCAAAAAATCGAATGTGGAAGGAGCTCAAGGGAGTGGAGAAGAAGCTGCCGAAGTTCTTGTGCGGCGCGACAATGGCGAATGTGGGGGAGAATTTGGTTGTTCTGTGGGAAACCAAGGGAAATGGGAAAGATATGGAGATTTGGTGTGCGGAGATTGAGGTGAAGGAAAATGGAGATGGGGAGTTGTGGGGGAGAATCGATTGGTCCCAGAAGCTTCTTTCGGTTCCAAAGCACTCCAACATTGTACAATGCTTGGCGGTTGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

361

Amino Acids

40.12

Weight (kDa)

8.7

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 81 - 344 5.7e-82 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 102 - 185 3.9e-07 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 103 - 243 1.7e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 109 - 152 2.2e-11 Kelch motif
Kelch_1 PF01344 155 - 200 1.7e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 329
AccBSI CCGCTC 3 cut(s) 138, 174, 219
AccII CGCG 6 cut(s) 234, 389, 456, 463, 632, 884
AclWI GGATC 2 cut(s) 82, 95
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 3 cut(s) 26, 498, 907
AcyI GRCGYC 3 cut(s) 63, 279, 330
AfaI GTAC 1 cut(s) 1062
AfiI CCNNNNNNNGG 8 cut(s) 319, 326, 574, 575, 607, 679, 708, 709
AhdI GACNNNNNGTC 1 cut(s) 391
AjiI CACGTC 1 cut(s) 95
AjnI CCWGG 3 cut(s) 239, 431, 536
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
AleI CACNNNNGTG 1 cut(s) 633
AluBI AGCT 6 cut(s) 21, 40, 83, 841, 862, 1030
AluI AGCT 6 cut(s) 21, 40, 83, 841, 862, 1030
Alw21I GWGCWC 2 cut(s) 23, 843
AlwI GGATC 2 cut(s) 82, 95
AlwNI CAGNNNCTG 1 cut(s) 479
Ama87I CYCGRG 3 cut(s) 568, 694, 702
AoxI GGCC 5 cut(s) 321, 349, 443, 552, 588
ApeKI GCWGC 2 cut(s) 473, 862
ApoI RAATTY 3 cut(s) 26, 498, 907
AspLEI GCGC 7 cut(s) 236, 332, 391, 456, 463, 664, 884
AspS9I GGNCC 8 cut(s) 350, 375, 409, 443, 534, 552, 670, 1020
AsuC2I CCSGG 9 cut(s) 320, 353, 442, 569, 570, 602, 674, 703, 704
AsuHPI GGTGA 4 cut(s) 157, 202, 652, 988
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 3 cut(s) 568, 694, 702
AvaII GGWCC 5 cut(s) 375, 409, 534, 670, 1020
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 3 cut(s) 23, 602, 843
Bbv12I GWGCWC 2 cut(s) 23, 843
BbvI GCAGC 2 cut(s) 485, 849
BccI CCATC 4 cut(s) 140, 308, 724, 986
BceAI ACGGC 7 cut(s) 48, 266, 308, 326, 376, 508, 575
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciT130I CCWGG 3 cut(s) 241, 433, 538
BcnI CCSGG 9 cut(s) 320, 353, 442, 569, 570, 602, 674, 703, 704
BfaI CTAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 333
BglII AGATCT 1 cut(s) 357
BisI GCNGC 7 cut(s) 333, 421, 446, 474, 477, 863, 880
BlsI GCNGC 7 cut(s) 334, 422, 447, 475, 478, 864, 881
Bme18I GGWCC 5 cut(s) 375, 409, 534, 670, 1020
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 3 cut(s) 568, 694, 702
BmgBI CACGTC 1 cut(s) 95
BmgT120I GGNCC 8 cut(s) 350, 375, 409, 443, 534, 552, 670, 1020
BmiI GGNNCC 6 cut(s) 89, 331, 376, 410, 1022, 1041
BmrI ACTGGG 1 cut(s) 559
BmsI GCATC 3 cut(s) 441, 506, 615
BmuI ACTGGG 1 cut(s) 559
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 827
BpuMI CCSGG 9 cut(s) 320, 353, 442, 569, 570, 602, 674, 703, 704
Bsa29I ATCGAT 1 cut(s) 1014
BsaHI GRCGYC 3 cut(s) 63, 279, 330
BsaJI CCNNGG 6 cut(s) 367, 555, 568, 673, 702, 930
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 8 cut(s) 319, 326, 574, 575, 607, 679, 708, 709
Bse1I ACTGG 1 cut(s) 554
BseBI CCWGG 3 cut(s) 241, 433, 538
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 6 cut(s) 367, 555, 568, 673, 702, 930
BseGI GGATG 6 cut(s) 132, 300, 521, 630, 735, 777
BseLI CCNNNNNNNGG 8 cut(s) 319, 326, 574, 575, 607, 679, 708, 709
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 5 cut(s) 134, 139, 170, 191, 281
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 2 cut(s) 485, 849
BseYI CCCAGC 1 cut(s) 479
Bsh1236I CGCG 6 cut(s) 234, 389, 456, 463, 632, 884
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 5 cut(s) 323, 351, 445, 554, 590
BshNI GGYRCC 1 cut(s) 329
BshVI ATCGAT 1 cut(s) 1014
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 2 cut(s) 23, 843
BsiHKCI CYCGRG 3 cut(s) 568, 694, 702
BsiSI CCGG 7 cut(s) 320, 353, 441, 569, 602, 673, 703
BslFI GGGAC 4 cut(s) 281, 296, 302, 1006
BslI CCNNNNNNNGG 8 cut(s) 319, 326, 574, 575, 607, 679, 708, 709
BsmFI GGGAC 4 cut(s) 281, 296, 302, 1006
BsnI GGCC 5 cut(s) 323, 351, 445, 554, 590
BsoBI CYCGRG 3 cut(s) 568, 694, 702
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 23, 602, 843
Bsp1407I TGTACA 1 cut(s) 1060
Bsp143I GATC 3 cut(s) 87, 357, 507
BspANI GGCC 5 cut(s) 323, 351, 445, 554, 590
BspCNI CTCAG 1 cut(s) 35
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 6 cut(s) 234, 389, 456, 463, 632, 884
BspLI GGNNCC 6 cut(s) 89, 331, 376, 410, 1022, 1041
BspPI GGATC 2 cut(s) 82, 95
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 329
BsrBI CCGCTC 3 cut(s) 138, 174, 219
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 6 cut(s) 367, 555, 568, 673, 702, 930
BssMI GATC 3 cut(s) 87, 357, 507
BssNI GRCGYC 3 cut(s) 63, 279, 330
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 3 cut(s) 241, 433, 538
Bst6I CTCTTC 1 cut(s) 719
BstACI GRCGYC 3 cut(s) 63, 279, 330
BstAUI TGTACA 1 cut(s) 1060
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 438, 630, 666, 757
BstDEI CTNAG 1 cut(s) 22
BstF5I GGATG 6 cut(s) 132, 300, 521, 630, 735, 777
BstFNI CGCG 6 cut(s) 234, 389, 456, 463, 632, 884
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 7 cut(s) 236, 332, 391, 456, 463, 664, 884
BstKTI GATC 3 cut(s) 90, 360, 510
BstMBI GATC 3 cut(s) 87, 357, 507
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 6 cut(s) 329, 338, 442, 451, 460, 890
BstNI CCWGG 3 cut(s) 241, 433, 538
BstUI CGCG 6 cut(s) 234, 389, 456, 463, 632, 884
BstV1I GCAGC 2 cut(s) 485, 849
BstX2I RGATCY 2 cut(s) 87, 357
BstYI RGATCY 2 cut(s) 87, 357
BstZI CGGCCG 1 cut(s) 321
Bsu15I ATCGAT 1 cut(s) 1014
BsuRI GGCC 5 cut(s) 323, 351, 445, 554, 590
BsuTUI ATCGAT 1 cut(s) 1014
BtrI CACGTC 1 cut(s) 95
BtsCI GGATG 6 cut(s) 132, 300, 521, 630, 735, 777
Cac8I GCNNGC 4 cut(s) 438, 630, 666, 757
CaiI CAGNNNCTG 1 cut(s) 479
CfoI GCGC 7 cut(s) 236, 332, 391, 456, 463, 664, 884
Cfr13I GGNCC 8 cut(s) 350, 375, 409, 443, 534, 552, 670, 1020
Cfr9I CCCGGG 2 cut(s) 568, 702
ClaI ATCGAT 1 cut(s) 1014
CpoI CGGWCCG 1 cut(s) 670
CseI GACGC 3 cut(s) 287, 395, 824
CsiI ACCWGGT 2 cut(s) 239, 536
Csp6I GTAC 1 cut(s) 1061
CspI CGGWCCG 1 cut(s) 670
CviAII CATG 2 cut(s) 736, 767
CviQI GTAC 1 cut(s) 1061
DdeI CTNAG 1 cut(s) 22
DinI GGCGCC 1 cut(s) 331
DpnI GATC 3 cut(s) 89, 359, 509
DpnII GATC 3 cut(s) 87, 357, 507
DriI GACNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 321
EagI CGGCCG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 719
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 719
EciI GGCGGA 4 cut(s) 50, 178, 275, 683
Ecl136II GAGCTC 2 cut(s) 21, 841
EclXI CGGCCG 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 930
Eco24I GRGCYC 3 cut(s) 23, 602, 843
Eco47I GGWCC 5 cut(s) 375, 409, 534, 670, 1020
Eco52I CGGCCG 1 cut(s) 321
Eco53kI GAGCTC 2 cut(s) 21, 841
Eco88I CYCGRG 3 cut(s) 568, 694, 702
EcoICRI GAGCTC 2 cut(s) 21, 841
EcoO109I RGGNCCY 1 cut(s) 409
EcoRI GAATTC 1 cut(s) 26
EcoRII CCWGG 3 cut(s) 239, 431, 536
EcoT14I CCWWGG 1 cut(s) 930
EcoT38I GRGCYC 3 cut(s) 23, 602, 843
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 930
FaeI CATG 2 cut(s) 739, 770
FaiI YATR 8 cut(s) 58, 339, 660, 690, 737, 768, 786, 950
FaqI GGGAC 4 cut(s) 281, 296, 302, 1006
FatI CATG 2 cut(s) 735, 766
FauI CCCGC 5 cut(s) 179, 312, 365, 645, 671
Fnu4HI GCNGC 7 cut(s) 333, 421, 446, 474, 477, 863, 880
FokI GGATG 6 cut(s) 119, 287, 528, 637, 742, 784
FriOI GRGCYC 3 cut(s) 23, 602, 843
Fsp4HI GCNGC 7 cut(s) 333, 421, 446, 474, 477, 863, 880
FspBI CTAG 1 cut(s) 80
GlaI GCGC 7 cut(s) 235, 331, 390, 455, 462, 663, 883
GluI GCNGC 7 cut(s) 333, 421, 446, 474, 477, 863, 880
GsaI CCCAGC 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 5 cut(s) 323, 351, 445, 554, 590
HapII CCGG 7 cut(s) 320, 353, 441, 569, 602, 673, 703
HgaI GACGC 3 cut(s) 287, 395, 824
HhaI GCGC 7 cut(s) 236, 332, 391, 456, 463, 664, 884
Hin1I GRCGYC 3 cut(s) 63, 279, 330
Hin1II CATG 2 cut(s) 739, 770
Hin6I GCGC 7 cut(s) 234, 330, 389, 454, 461, 662, 882
HinP1I GCGC 7 cut(s) 234, 330, 389, 454, 461, 662, 882
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 2 cut(s) 527, 1011
HpaII CCGG 7 cut(s) 320, 353, 441, 569, 602, 673, 703
HphI GGTGA 4 cut(s) 157, 202, 652, 988
Hpy166II GTNNAC 3 cut(s) 6, 382, 490
Hpy188I TCNGA 2 cut(s) 25, 87
Hpy188III TCNNGA 3 cut(s) 224, 505, 563
Hpy8I GTNNAC 3 cut(s) 6, 382, 490
Hpy99I CGWCG 7 cut(s) 65, 68, 281, 329, 347, 350, 389
HpyAV CCTTC 2 cut(s) 829, 973
HpyCH4IV ACGT 4 cut(s) 63, 94, 363, 402
HpyCH4V TGCA 2 cut(s) 628, 766
HpyF10VI GCNNNNNNNGC 6 cut(s) 329, 338, 442, 451, 460, 890
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 4 cut(s) 63, 94, 363, 402
Hsp92I GRCGYC 3 cut(s) 63, 279, 330
Hsp92II CATG 2 cut(s) 739, 770
HspAI GCGC 7 cut(s) 234, 330, 389, 454, 461, 662, 882
KasI GGCGCC 1 cut(s) 329
Kzo9I GATC 3 cut(s) 87, 357, 507
LmnI GCTCC 3 cut(s) 37, 179, 838
Lsp1109I GCAGC 2 cut(s) 485, 849
LweI GCATC 3 cut(s) 441, 506, 615
MabI ACCWGGT 2 cut(s) 239, 536
MaeI CTAG 1 cut(s) 80
MaeII ACGT 4 cut(s) 63, 94, 363, 402
MaeIII GTNAC 2 cut(s) 95, 520
MalI GATC 3 cut(s) 89, 359, 509
MbiI CCGCTC 3 cut(s) 138, 174, 219
MboI GATC 3 cut(s) 87, 357, 507
MboII GAAGA 5 cut(s) 151, 154, 736, 811, 868
MflI RGATCY 2 cut(s) 87, 357
MhlI GDGCHC 3 cut(s) 23, 602, 843
MluCI AATT 3 cut(s) 26, 498, 907
Mly113I GGCGCC 1 cut(s) 330
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 1077
MslI CAYNNNNRTG 1 cut(s) 633
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 7 cut(s) 320, 353, 441, 569, 602, 673, 703
MvaI CCWGG 3 cut(s) 241, 433, 538
MvnI CGCG 6 cut(s) 234, 389, 456, 463, 632, 884
MwoI GCNNNNNNNGC 6 cut(s) 329, 338, 442, 451, 460, 890
NarI GGCGCC 1 cut(s) 330
NciI CCSGG 9 cut(s) 320, 353, 442, 569, 570, 602, 674, 703, 704
NdeII GATC 3 cut(s) 87, 357, 507
NlaIII CATG 2 cut(s) 739, 770
NlaIV GGNNCC 6 cut(s) 89, 331, 376, 410, 1022, 1041
NmeAIII GCCGAG 3 cut(s) 348, 580, 621
NmuCI GTSAC 2 cut(s) 95, 520
NspV TTCGAA 1 cut(s) 71
OliI CACNNNNGTG 1 cut(s) 633
PcsI WCGNNNNNNNCGW 3 cut(s) 91, 375, 516
PfeI GAWTC 1 cut(s) 1011
PflFI GACNNNGTC 2 cut(s) 281, 538
PkrI GCNGC 7 cut(s) 334, 422, 447, 475, 478, 864, 881
PleI GAGTC 1 cut(s) 521
PluTI GGCGCC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 521
PpuMI RGGWCCY 1 cut(s) 409
Psp124BI GAGCTC 2 cut(s) 23, 843
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 3 cut(s) 239, 431, 536
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 3 cut(s) 239, 431, 536
PspN4I GGNNCC 6 cut(s) 89, 331, 376, 410, 1022, 1041
PspPI GGNCC 8 cut(s) 350, 375, 409, 443, 534, 552, 670, 1020
PspPPI RGGWCCY 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 2 cut(s) 87, 357
PsyI GACNNNGTC 2 cut(s) 281, 538
RsaI GTAC 1 cut(s) 1062
RsaNI GTAC 1 cut(s) 1061
RseI CAYNNNNRTG 1 cut(s) 633
Rsr2I CGGWCCG 1 cut(s) 670
RsrII CGGWCCG 1 cut(s) 670
SacI GAGCTC 2 cut(s) 23, 843
SatI GCNGC 7 cut(s) 333, 421, 446, 474, 477, 863, 880
Sau3AI GATC 3 cut(s) 87, 357, 507
Sau96I GGNCC 8 cut(s) 350, 375, 409, 443, 534, 552, 670, 1020
SchI GAGTC 1 cut(s) 521
SduI GDGCHC 3 cut(s) 23, 602, 843
SexAI ACCWGGT 2 cut(s) 239, 536
SfaNI GCATC 3 cut(s) 441, 506, 615
SfoI GGCGCC 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 5 cut(s) 375, 409, 534, 670, 1020
SmaI CCCGGG 2 cut(s) 570, 704
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
Sse9I AATT 3 cut(s) 26, 498, 907
SspDI GGCGCC 1 cut(s) 329
SspMI CTAG 1 cut(s) 80
SstI GAGCTC 2 cut(s) 23, 843
StyI CCWWGG 1 cut(s) 930
TaiI ACGT 4 cut(s) 66, 97, 366, 405
TaqI TCGA 6 cut(s) 71, 414, 542, 564, 826, 1014
TasI AATT 3 cut(s) 26, 498, 907
TatI WGTACW 1 cut(s) 1060
TauI GCSGC 5 cut(s) 335, 423, 448, 479, 882
TfiI GAWTC 1 cut(s) 1011
TseFI GTSAC 2 cut(s) 95, 520
TseI GCWGC 2 cut(s) 473, 862
Tsp45I GTSAC 2 cut(s) 95, 520
TspGWI ACGGA 2 cut(s) 367, 539
TspMI CCCGGG 2 cut(s) 568, 702
Tth111I GACNNNGTC 2 cut(s) 281, 538
VpaK11BI GGWCC 5 cut(s) 375, 409, 534, 670, 1020
XapI RAATTY 3 cut(s) 26, 498, 907
XmaI CCCGGG 2 cut(s) 568, 702
XspI CTAG 1 cut(s) 80
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.