pycom15g29250

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010182
Physical Location & Seq
Forward (+)
25974553 .. 25975650
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29250.1

Sequence Viewer

Length: 1098 bp
ATGTCCACCTCCACCTCCACCACCGCCGCCGCCGCCGCAACGGCACAACTGATCCCATCCCTCCCAGACGACGTCGCTTTGAACTGCATAGCCCGAGTCCCCCGCTGGTACCACCCGACTCTGTCGATAGTGTCGAGACCCATCCGCTCCCTCCTCTCATCCCCACTCTTCTTCACCACCCGCTCCCTCCTCAATTCAACCCAACATGTCCTCTACCTCACCCTCCGCTCACACCATAACCCCTCCGCCTGGTTCACACTCTACCAAAACCCCAACCCCACCGACCTCCCTCCTCTTCTCATCCCCGTTCCACCCATCCCCGCCGCATCACTCGGCGCCGCCTACGCCGTGCTGGGCTCCACCATCTACGTCCTCGGTGGCTCAGTCAACGACGTCCCCTCCTCCCACGTGTGGCTCCTCGACTGCCGCTTTCACACCTGGCAACAGGGCCCCCCTATGCGCGTGGCGCGCGAGTTCGCCGCCGCGGGTGTTGTGGACGGAAGGGTCTACGTCATCGGCGGCTGCCTTGTGGACTCGTGGGCGCGGACCGAGCACTGGGCGGAGGTTCTAGACCCAGAGAAGGGCCGCTGGGAGGCGGTGCCGAGTTCTGCAATTGAGGTCCGTGGCAAGTGGATGCACGCGAGCGCTGTGATCGGCGGCCGCGTGTATGCCATGGCAGACCGGGGCGGGGTAGTTTTCGATCCAAAAACTGGGGTTTGGGAAGGAGTTGAGAAAAGGCTTGATTTGGGGTGGAGAGGGAGGGCTTGTGTGGTCCATGGGATTCTGTACTGCTATGACTATTTGGGGAAAATCAGAGGGTTTGATGTGAAGAAGAGGGCTTGGAAGGAGCTCAAGGGTGTGGAGAAGGGGCTGCCCAAGTTTTTGTGCGGTGCTACGATGGCGAATGTGGGAGACAAATTGGTTGTGTTGTGGGAAGGGAAGGGAAATGGCAAAGAAATTGAGATTTGGTGTGCTGAAATTGAGTTGGAGGAAAAGGGAGATGATGGGGAGCTGTGGGGAAACATCGATTGGTCCCATAAGCTTCTTTCAGTTCCGAAAGGCTCTTCCATCGTCAACTGCTTGGCAGTTTCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

366

Amino Acids

39.99

Weight (kDa)

8.07

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 84 - 348 6.9e-83 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 103 - 230 3e-09 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 106 - 244 5.3e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 112 - 154 1.2e-09 Kelch motif
Kelch_1 PF01344 157 - 202 6.7e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 503
AatII GACGTC 2 cut(s) 75, 396
Acc65I GGTACC 1 cut(s) 108
AccB1I GGYRCC 3 cut(s) 108, 335, 598
AccB7I CCANNNNNTGG 1 cut(s) 710
AccBSI CCGCTC 3 cut(s) 147, 183, 228
AccI GTMKAC 1 cut(s) 507
AccII CGCG 7 cut(s) 462, 469, 471, 485, 544, 641, 663
AclWI GGATC 2 cut(s) 46, 695
AcoI YGGCCR 1 cut(s) 658
AcvI CACGTG 1 cut(s) 409
AcyI GRCGYC 3 cut(s) 72, 336, 393
AfaI GTAC 2 cut(s) 110, 788
AfeI AGCGCT 1 cut(s) 646
AfiI CCNNNNNNNGG 8 cut(s) 249, 411, 555, 580, 581, 592, 688, 710
AflIII ACRYGT 2 cut(s) 205, 408
AgsI TTSAA 2 cut(s) 82, 198
AjnI CCWGG 2 cut(s) 248, 437
AjuI GAANNNNNNNTTGG 2 cut(s) 1012, 1044
AluBI AGCT 3 cut(s) 850, 1012, 1042
AluI AGCT 3 cut(s) 850, 1012, 1042
Alw21I GWGCWC 2 cut(s) 555, 852
Alw26I GTCTC 2 cut(s) 130, 906
AlwI GGATC 2 cut(s) 46, 695
Ama87I CYCGRG 1 cut(s) 93
Aor51HI AGCGCT 1 cut(s) 646
AoxI GGCC 3 cut(s) 448, 583, 658
ApaI GGGCCC 1 cut(s) 452
ApeKI GCWGC 2 cut(s) 522, 871
Asp718I GGTACC 1 cut(s) 108
AspLEI GCGC 6 cut(s) 338, 462, 469, 471, 544, 647
AspS9I GGNCC 7 cut(s) 448, 449, 546, 583, 619, 772, 1032
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 2 cut(s) 166, 211
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 4 cut(s) 546, 619, 772, 1032
BaeGI GKGCMC 1 cut(s) 452
BanI GGYRCC 3 cut(s) 108, 335, 598
BanII GRGCYC 3 cut(s) 359, 452, 852
BauI CACGAG 1 cut(s) 535
BbrPI CACGTG 1 cut(s) 409
Bbv12I GWGCWC 2 cut(s) 555, 852
BbvI GCAGC 2 cut(s) 509, 858
BccI CCATC 7 cut(s) 64, 149, 323, 371, 892, 998, 1076
BceAI ACGGC 2 cut(s) 57, 332
BciT130I CCWGG 2 cut(s) 250, 439
BcnI CCSGG 1 cut(s) 683
BcoDI GTCTC 2 cut(s) 130, 906
BfaI CTAG 1 cut(s) 569
BfoI RGCGCY 2 cut(s) 339, 648
BglI GCCNNNNNGGC 1 cut(s) 41
Bme1390I CCNGG 3 cut(s) 250, 439, 683
Bme18I GGWCC 4 cut(s) 546, 619, 772, 1032
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 7 cut(s) 448, 449, 546, 583, 619, 772, 1032
BmiI GGNNCC 8 cut(s) 110, 337, 358, 416, 450, 451, 600, 1034
BmrFI CCNGG 3 cut(s) 250, 439, 683
BmrI ACTGGG 2 cut(s) 565, 720
BmsI GCATC 2 cut(s) 335, 624
BmuI ACTGGG 2 cut(s) 565, 720
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 1 cut(s) 683
Bsa29I ATCGAT 1 cut(s) 1026
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 3 cut(s) 72, 336, 393
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 6 cut(s) 373, 483, 622, 672, 682, 775
BsaXI ACNNNNNCTCC 4 cut(s) 383, 413, 751, 781
Bsc4I CCNNNNNNNGG 8 cut(s) 249, 411, 555, 580, 581, 592, 688, 710
Bse1I ACTGG 2 cut(s) 560, 715
BseBI CCWGG 2 cut(s) 250, 439
BseCI ATCGAT 1 cut(s) 1026
BseDI CCNNGG 6 cut(s) 373, 483, 622, 672, 682, 775
BseGI GGATG 6 cut(s) 56, 141, 158, 300, 315, 639
BseLI CCNNNNNNNGG 8 cut(s) 249, 411, 555, 580, 581, 592, 688, 710
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 560, 715
BsePI GCGCGC 1 cut(s) 467
BseRI GAGGAG 5 cut(s) 143, 179, 282, 391, 407
BseSI GKGCMC 1 cut(s) 452
BseX3I CGGCCG 1 cut(s) 658
BseXI GCAGC 2 cut(s) 509, 858
BseYI CCCAGC 2 cut(s) 352, 588
Bsh1236I CGCG 7 cut(s) 462, 469, 471, 485, 544, 641, 663
Bsh1285I CGRYCG 1 cut(s) 661
BshFI GGCC 3 cut(s) 450, 585, 660
BshNI GGYRCC 3 cut(s) 108, 335, 598
BshVI ATCGAT 1 cut(s) 1026
BsiEI CGRYCG 1 cut(s) 661
BsiHKAI GWGCWC 2 cut(s) 555, 852
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 3 cut(s) 83, 380, 1018
BslI CCNNNNNNNGG 8 cut(s) 249, 411, 555, 580, 581, 592, 688, 710
BsmAI GTCTC 2 cut(s) 130, 906
BsmFI GGGAC 3 cut(s) 83, 380, 1018
BsnI GGCC 3 cut(s) 450, 585, 660
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 93
Bsp120I GGGCCC 1 cut(s) 448
Bsp1286I GDGCHC 4 cut(s) 359, 452, 555, 852
Bsp143I GATC 3 cut(s) 51, 651, 700
Bsp19I CCATGG 2 cut(s) 672, 775
BspANI GGCC 3 cut(s) 450, 585, 660
BspCNI CTCAG 1 cut(s) 395
BspDI ATCGAT 1 cut(s) 1026
BspFNI CGCG 7 cut(s) 462, 469, 471, 485, 544, 641, 663
BspLI GGNNCC 8 cut(s) 110, 337, 358, 416, 450, 451, 600, 1034
BspPI GGATC 2 cut(s) 46, 695
BspQI GCTCTTC 1 cut(s) 1069
BspT107I GGYRCC 3 cut(s) 108, 335, 598
BspTNI GGTCTC 1 cut(s) 130
BsrBI CCGCTC 3 cut(s) 147, 183, 228
BsrI ACTGG 2 cut(s) 560, 715
BssECI CCNNGG 6 cut(s) 373, 483, 622, 672, 682, 775
BssHII GCGCGC 1 cut(s) 467
BssMI GATC 3 cut(s) 51, 651, 700
BssNI GRCGYC 3 cut(s) 72, 336, 393
BssSI CACGAG 1 cut(s) 535
BssT1I CCWWGG 2 cut(s) 672, 775
Bst2BI CACGAG 1 cut(s) 535
Bst2UI CCWGG 2 cut(s) 250, 439
Bst6I CTCTTC 4 cut(s) 173, 300, 827, 1069
BstACI GRCGYC 3 cut(s) 72, 336, 393
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 3 cut(s) 469, 639, 643
BstDEI CTNAG 1 cut(s) 382
BstDSI CCRYGG 4 cut(s) 483, 622, 672, 775
BstF5I GGATG 6 cut(s) 56, 141, 158, 300, 315, 639
BstFNI CGCG 7 cut(s) 462, 469, 471, 485, 544, 641, 663
BstH2I RGCGCY 2 cut(s) 339, 648
BstHHI GCGC 6 cut(s) 338, 462, 469, 471, 544, 647
BstKTI GATC 3 cut(s) 54, 654, 703
BstMAI GTCTC 2 cut(s) 130, 906
BstMBI GATC 3 cut(s) 51, 651, 700
BstMCI CGRYCG 1 cut(s) 661
BstMWI GCNNNNNNNGC 8 cut(s) 32, 35, 41, 344, 466, 468, 550, 899
BstNI CCWGG 2 cut(s) 250, 439
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 3 cut(s) 248, 437, 681
BstSLI GKGCMC 1 cut(s) 452
BstUI CGCG 7 cut(s) 462, 469, 471, 485, 544, 641, 663
BstV1I GCAGC 2 cut(s) 509, 858
BstZI CGGCCG 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 450, 585, 660
BsuTUI ATCGAT 1 cut(s) 1026
BtgI CCRYGG 4 cut(s) 483, 622, 672, 775
BtsCI GGATG 6 cut(s) 56, 141, 158, 300, 315, 639
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 3 cut(s) 469, 639, 643
CciNI GCGGCCGC 1 cut(s) 658
CfoI GCGC 6 cut(s) 338, 462, 469, 471, 544, 647
Cfr13I GGNCC 7 cut(s) 448, 449, 546, 583, 619, 772, 1032
Cfr42I CCGCGG 1 cut(s) 486
ClaI ATCGAT 1 cut(s) 1026
CpoI CGGWCCG 1 cut(s) 546
Csp6I GTAC 2 cut(s) 109, 787
CspI CGGWCCG 1 cut(s) 546
CviAII CATG 3 cut(s) 206, 673, 776
CviQI GTAC 2 cut(s) 109, 787
DdeI CTNAG 1 cut(s) 382
DinI GGCGCC 1 cut(s) 337
DpnI GATC 3 cut(s) 53, 653, 702
DpnII GATC 3 cut(s) 51, 651, 700
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 658
EagI CGGCCG 1 cut(s) 658
Eam1104I CTCTTC 4 cut(s) 173, 300, 827, 1069
EarI CTCTTC 4 cut(s) 173, 300, 827, 1069
EciI GGCGGA 2 cut(s) 235, 575
Ecl136II GAGCTC 1 cut(s) 850
EclXI CGGCCG 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 672, 775
Eco24I GRGCYC 3 cut(s) 359, 452, 852
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 4 cut(s) 546, 619, 772, 1032
Eco47III AGCGCT 1 cut(s) 646
Eco52I CGGCCG 1 cut(s) 658
Eco53kI GAGCTC 1 cut(s) 850
Eco72I CACGTG 1 cut(s) 409
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 2 cut(s) 448, 449
EcoRII CCWGG 2 cut(s) 248, 437
EcoT14I CCWWGG 2 cut(s) 672, 775
EcoT38I GRGCYC 3 cut(s) 359, 452, 852
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
ErhI CCWWGG 2 cut(s) 672, 775
FaeI CATG 3 cut(s) 209, 676, 779
FaiI YATR 9 cut(s) 89, 207, 237, 458, 669, 674, 777, 795, 1038
FaqI GGGAC 3 cut(s) 83, 380, 1018
FatI CATG 3 cut(s) 205, 672, 775
FauI CCCGC 5 cut(s) 110, 188, 328, 478, 680
FblI GTMKAC 1 cut(s) 507
FokI GGATG 6 cut(s) 43, 128, 145, 287, 302, 646
FriOI GRGCYC 3 cut(s) 359, 452, 852
FspBI CTAG 1 cut(s) 569
GlaI GCGC 6 cut(s) 337, 461, 468, 470, 543, 646
GsaI CCCAGC 2 cut(s) 356, 592
HaeII RGCGCY 2 cut(s) 339, 648
HaeIII GGCC 3 cut(s) 450, 585, 660
HapII CCGG 1 cut(s) 682
HhaI GCGC 6 cut(s) 338, 462, 469, 471, 544, 647
Hin1I GRCGYC 3 cut(s) 72, 336, 393
Hin1II CATG 3 cut(s) 209, 676, 779
Hin6I GCGC 6 cut(s) 336, 460, 467, 469, 542, 645
HinP1I GCGC 6 cut(s) 336, 460, 467, 469, 542, 645
HincII GTYRAC 2 cut(s) 388, 1075
HindII GTYRAC 2 cut(s) 388, 1075
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 4 cut(s) 96, 118, 533, 781
HpaII CCGG 1 cut(s) 682
HphI GGTGA 2 cut(s) 166, 211
Hpy166II GTNNAC 7 cut(s) 6, 255, 388, 496, 508, 532, 1075
Hpy188I TCNGA 2 cut(s) 815, 1056
Hpy188III TCNNGA 2 cut(s) 135, 569
Hpy8I GTNNAC 7 cut(s) 6, 255, 388, 496, 508, 532, 1075
Hpy99I CGWCG 3 cut(s) 74, 77, 395
HpyAV CCTTC 7 cut(s) 495, 574, 716, 838, 859, 929, 934
HpyCH4IV ACGT 5 cut(s) 72, 369, 393, 408, 510
HpyCH4V TGCA 3 cut(s) 87, 611, 637
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 35, 41, 344, 466, 468, 550, 899
HpyF3I CTNAG 1 cut(s) 382
HpySE526I ACGT 5 cut(s) 72, 369, 393, 408, 510
Hsp92I GRCGYC 3 cut(s) 72, 336, 393
Hsp92II CATG 3 cut(s) 209, 676, 779
HspAI GCGC 6 cut(s) 336, 460, 467, 469, 542, 645
KasI GGCGCC 1 cut(s) 335
KpnI GGTACC 1 cut(s) 112
KspI CCGCGG 1 cut(s) 486
Kzo9I GATC 3 cut(s) 51, 651, 700
LguI GCTCTTC 1 cut(s) 1069
LmnI GCTCC 6 cut(s) 152, 188, 362, 420, 847, 1009
Lsp1109I GCAGC 2 cut(s) 509, 858
LweI GCATC 2 cut(s) 335, 624
MaeI CTAG 1 cut(s) 569
MaeII ACGT 5 cut(s) 72, 369, 393, 408, 510
MalI GATC 3 cut(s) 53, 653, 702
MbiI CCGCTC 3 cut(s) 147, 183, 228
MboI GATC 3 cut(s) 51, 651, 700
MboII GAAGA 6 cut(s) 160, 163, 287, 841, 844, 1056
MfeI CAATTG 1 cut(s) 612
MhlI GDGCHC 4 cut(s) 359, 452, 555, 852
MluCI AATT 5 cut(s) 193, 612, 917, 957, 978
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 3 cut(s) 105, 112, 527
MmeI TCCRAC 1 cut(s) 966
MspA1I CMGCKG 3 cut(s) 105, 485, 588
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 3 cut(s) 250, 439, 683
MunI CAATTG 1 cut(s) 612
MvaI CCWGG 2 cut(s) 250, 439
MvnI CGCG 7 cut(s) 462, 469, 471, 485, 544, 641, 663
MwoI GCNNNNNNNGC 8 cut(s) 32, 35, 41, 344, 466, 468, 550, 899
NarI GGCGCC 1 cut(s) 336
NciI CCSGG 1 cut(s) 683
NcoI CCATGG 2 cut(s) 672, 775
NdeII GATC 3 cut(s) 51, 651, 700
NlaIII CATG 3 cut(s) 209, 676, 779
NlaIV GGNNCC 8 cut(s) 110, 337, 358, 416, 450, 451, 600, 1034
NmeAIII GCCGAG 2 cut(s) 312, 627
NotI GCGGCCGC 1 cut(s) 658
NspI RCATGY 1 cut(s) 209
PauI GCGCGC 1 cut(s) 467
PciI ACATGT 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 1069
PcsI WCGNNNNNNNCGW 2 cut(s) 131, 660
PfeI GAWTC 1 cut(s) 781
PflFI GACNNNGTC 2 cut(s) 71, 121
PflMI CCANNNNNTGG 1 cut(s) 710
PleI GAGTC 3 cut(s) 104, 112, 527
PluTI GGCGCC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 409
PmlI CACGTG 1 cut(s) 409
PpsI GAGTC 3 cut(s) 104, 112, 527
Ppu21I YACGTR 1 cut(s) 409
PscI ACATGT 1 cut(s) 205
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 248, 437
PspCI CACGTG 1 cut(s) 409
PspFI CCCAGC 2 cut(s) 352, 588
PspGI CCWGG 2 cut(s) 248, 437
PspN4I GGNNCC 8 cut(s) 110, 337, 358, 416, 450, 451, 600, 1034
PspOMI GGGCCC 1 cut(s) 448
PspPI GGNCC 7 cut(s) 448, 449, 546, 583, 619, 772, 1032
PsyI GACNNNGTC 2 cut(s) 71, 121
PteI GCGCGC 1 cut(s) 467
RsaI GTAC 2 cut(s) 110, 788
RsaNI GTAC 2 cut(s) 109, 787
Rsr2I CGGWCCG 1 cut(s) 546
RsrII CGGWCCG 1 cut(s) 546
SacI GAGCTC 1 cut(s) 852
SacII CCGCGG 1 cut(s) 486
SapI GCTCTTC 1 cut(s) 1069
Sau3AI GATC 3 cut(s) 51, 651, 700
Sau96I GGNCC 7 cut(s) 448, 449, 546, 583, 619, 772, 1032
SchI GAGTC 3 cut(s) 105, 112, 527
ScrFI CCNGG 3 cut(s) 250, 439, 683
SduI GDGCHC 4 cut(s) 359, 452, 555, 852
SfaNI GCATC 2 cut(s) 335, 624
SfoI GGCGCC 1 cut(s) 337
Sfr303I CCGCGG 1 cut(s) 486
SgrBI CCGCGG 1 cut(s) 486
SinI GGWCC 4 cut(s) 546, 619, 772, 1032
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
Sse9I AATT 5 cut(s) 193, 612, 917, 957, 978
SspDI GGCGCC 1 cut(s) 335
SspMI CTAG 1 cut(s) 569
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 3 cut(s) 248, 437, 681
StyI CCWWGG 2 cut(s) 672, 775
TaiI ACGT 5 cut(s) 75, 372, 396, 411, 513
TaqI TCGA 5 cut(s) 125, 134, 420, 699, 1026
TaqII GACCGA 1 cut(s) 563
TasI AATT 5 cut(s) 193, 612, 917, 957, 978
TatI WGTACW 1 cut(s) 786
TfiI GAWTC 1 cut(s) 781
TscAI CASTG 1 cut(s) 560
TseI GCWGC 2 cut(s) 522, 871
TspGWI ACGGA 2 cut(s) 513, 611
TspRI CASTG 1 cut(s) 560
Tth111I GACNNNGTC 2 cut(s) 71, 121
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 4 cut(s) 546, 619, 772, 1032
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 507
XspI CTAG 1 cut(s) 569
ZraI GACGTC 2 cut(s) 73, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.