Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002142

Physical Location & Seq

Genomic Coordinates Reverse (-)

9363109 .. 9369461

Gene Structure 6353 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G22010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3843 bp

ATGGCTGAAGACAGCCTACGGGTTGGTATGATTTCATCTGGTTTGGCTGTTTTACTGAATGGTGAAGATGCTAAAGAAAATTCCTCGAAAGCTCGTATTGTTCCACATTTTGATTATTCTGGTCACCGGCCTTTAGAACGGACTATCGAATTCATATTTGGTTTAGCAGAAAAGTCGGTTGGTCCATTGGATGGTCAAGTAGACAGTAGCTTGATTAGAGCTGTGATTAAGAATCAGTTCTCGAAGCTTCATGGTGATTTGGATGTTTCAGTTAGTCAGAGAGAAGGAATCAGTGTAGTTCATCATGGTGTTGGACCTCCTATTGTTGGCCTTGAAGAGTTTAGCATATGTGGTGATATCAGGATTGTGAAGCCACCTCTTGTATTGGAGAGCTTAGCACTTTTCAGTAGCGCCAGGGCTAATGCTTGTATTTGGAAAGGAAAATGGATGTATGAAGTCGCTTTAGAGACCTCGGGAATTCAACAACTTGGATGGGCAACACTTGCTTGCCCTTTCACTGACCAGAAAGGTGTGGGTGATGCTGATGATTCATATGCTTTTGATGGTCGAAGGGTAAGCAAATGGAACAAGGAAGCTGAACCTTATGGTCAATCATGGGTTGCTGGGGATGTCATCGGTTGCTGCATAGATTTGAACTGTGATGAGATTTACTTCTACAGGAATGGTGTCTCCCTCGGGGCTGCATTTACTGGTATTAGAAAACTTGGACCTGGATTTGGGTATTACCCTGCTATATCTCTCTCCCAAGGTGAGAGATGCGAGTTAAATTTCGGCGCTTACCCGTTTAAGTACCCTGTTGACGGTTTCCAACCTCTTCAAGAAGCTCCAACTCGATTTTCATTTGCAACAGAGCTACTACGATGCTTCTCAAGACTGCTGGATAGGCCAGACCGCTCATTGGCTGATACATTAAGTCGACTGAGGAGATTTGCCTCAGTCGAAGAACTCTTTTGCCCTGTTTCGTCTGCCATATGTGATGAGTTCTTTTATATACTTGAGCAAGACCCTTTGTTGGCGGAGTACTTAGGACGGGGTGCATTTCTGTCATTCCTCTTGGAGACTTTCAGAACTCAAGCACCGCATGATTCGTCAAGTTTAGACAAAGTTCTTGATGTGTTTCTTGAGTTCCCACAATCTCATTTGATCTTTGAGCATGTAGTAAACGCTCTGGCTTGTGGCTGCAAAACAGCGACACTGATTTTAACCGAGTGTCCATACTCCGGACCTTATCCCTATCTGGCGCTGGCTTGTCATTTGTTTAAACGGGAAGAATTGATGGTACAATGGTGGAGATCTTTGCATTTCGAGTTTTTGTTTGAAGGTTTCTTGTCATGCAGGAGTTCCAACAAGCATGACTTACAGCACTTAATGCCTGTTGTTTGGTGGCCTGGATCCTCTGAAGATATATCCTACGAGAGTAGCATGGGATTTACGATCTCCGCTCTCTCTGAAGCCATTAACAAGATTGAGGAGAAGCAGAGGAACCTCTGTCTCTTGGTCATACAATTCATACCACCTGTATCACCTCCCCAGCTTCCTGGTTCAGCTTTCAGGGGATTCCTACAGAACCTTTTATTGAAGAACAGAGGCGCAGATAGGACTCTGGCCCCTTCTGGAGTTACACGCAATTCGGTTCTTGTCTCTTTGTTCTCAGTTATACTCCACTTCTTGTCAGAAGGTTTTGCAATGCTGAAGAGTAGTGAAGCCGTCCATCACAATGTTGGTTTTCTTCACAGAGGTGGTCAGCAGAAGTTTCCATTGAGTTTGTTTCTTAAAAATGATCCACATCGCGCTGATATTACTAGGCTTGGCGGGCTATTTAGTCATATATCGAAGTCCTACCCCACAGATGATCAAGAAGAAGAAATAATGCGGTGGGAAGAAGGTTGTATGGATGATGAACAGAATAGAGTAACTCACGCGACTGAGCAAAAGCCATGCTGTTGCTTAGCTTATGATACCGATCTTACTAAATCCTTGAAGGATCGAGGAAAAAATACTGCTCAAAGTTCTCGTGGTCGATGCAGTTCCATTCCTGAGAGATCCTCTCATGTTGCTGCAGAATGTAGTGCTGGAAGTTTCAGTGAAGAAATAGACGACAAACCTAGCACAAGTAATCAGTCTGACCCTGACTTTGGTTATCGTCCAGTAAGATTTATGCGGACTGCACTTCAAGAGAGTAGAATCTCATCTGCTATACTAAGCGAAGAAGAACTATTGGATGCTCTATTGCTATTATACCATATTGCTGTTGCTCCAAATTTTAAGCAGGCATCATATTACATGTCTCACCAATCGCAATCAATATCACTTCTTGAAGAAACTGATAAACAGATAAGAGAACGGGCTTCTTGTGACCAAATAAAGCGCTTGAAGGAAGCTCGCAACAACTACAAAGAAGACGTGATGGACTGTGTTAGACATTCCGCATGGTTCCGCATCTCATTGTTCTCTCGATGGAAGCAAAGGGGAATGTATGCATTGTGCATGTGGGTTGTACAGCTACTTCTGGTTCTCAGCAAAATGGATTCTGTGTTTGTTTATATTCCAGAGTTCTACCTTGAATCTTTGGTAGACTGTTTCCATGTGCTTCGCAAGAGCGATCCTCCGTTTGTCCCATCAACAACATTCATCAAACAAGGACTTTCATCATTTATCACTTTCGTAGTTACACACTTCAACGATTCAAGGATATCGAATACAGATCTTAAAGATCTACTTCTCCAGTCAATATCCGTCCTGGTACAGTACAAAGAGTACTTGGAAGCTTTCGAGAACAATGAGGCAGCTACTCGACACATGCCTGCAGCACTACTGGCAGCGTTTGACAACAGATCTTGGATCCCTGTCACAAATATATTTCTTCGTCTTTGCAAAGGTTCTGGGTTTAGTTCTTTAAAGAACGGAGAGTCTTCGTTTTCGTCTACTGTTTTCCAGGCGCTTCTACGAGATGCTTGTATCAATGATGGTGAATTACTTTCTACTTTTCTTAACCGTCTATTCAACACACTGAGCTGGACCATCACTGAATTTTCTGTCTCGGTGCGAGAAATGCAAGAAAAGTATCAGGTAATGGAGTTTCAGCAAAGGAAATGTTGTGTGATATTTGAGCTTTCAAGCAATCTCGCAAGGGTCTTAGAATTCTGCACTTACGCAATGCCACAAGCATTTCTTGCTGGGACTGACACGAATCTTCGTAGGCTCACTGAGTTGATTCTTTTCATCTTGAATCACATGACCTCAGCAGTAGATGACGAGTTCTTTGATCTGTCACTTAGACGGCAGGGACAACCTTCAGAGAAAGTTAGCCGAGGGATCTTATTAGCTCCTTTGGTTGGTATAATCTTGAATCTCTTGGAGGCTAGTGAAGACTCAAAACCAAAGCAACAACATGATGTAATTGGTCTTTTCGCAAGCATGGACTGTCCCGATACAGTCTATTACGGGTTTCAGTATCTCTTGGAATATAATTGGGACGGTTGTGTAAGCGGGGATGATGCCTATGTAAAGAAACTTGGACAGCTTGAGAACTTCTTGAGCCATCTCATAAACCGGGCTTCATCGCAAGAACCCGAAAGAAAAGAAGAGTCATTCAACAAAGACACGACAGATATAGAAGACAACACTTGCTGTATCTGCTACGCGGGGGAGGCTAATGCAATGATTGCTCCGTGTTCACATCGATCATGTTATGGCTGCATAACTAGACATCTTCTCAACTGTCAGAGATGTTTTTTCTGCAATGCTACGGTGATAGATGTTATAAGAGATAAAGAAGAAGAAGAAGAAGAAGACGATGATGGTCACAAAAGAAGCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0060154 GO:0065007 GO:0070647 GO:0071704 GO:0098586 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPRY Ufd2P_core zf-C3HC4_3

Protein Analysis

1280

Amino Acids

144.5

Weight (kDa)

5.37

Isoelectric Point (pI)

51.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RKP_N	PF19322	1 - 137	3.9e-57	E3 ubiquitin-protein ligase RKP N-terminus
SPRY	PF00622	148 - 266	1.6e-29	SPRY domain
Ufd2P_core	PF10408	764 - 949	1.2e-07	Ubiquitin elongating factor core
TPR_RNF123	PF25576	1001 - 1195	4.4e-80	RNF123-like, TPR repeat
zf-C3HC4_3	PF13920	1213 - 1260	3.7e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22010 AT2G22010
fragaria_vesca	FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_7g04730 FvH4_7g04730
malus_domestica	MD01G1017400.v1.1 MD15G1333600.v1.1
prunus_persica	Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1
pyrus_communis	pycom01g04820 pycom01g04830 pycom15g29160 pycom15g29170
rosa_chinensis	RchiOBHm_Chr2g0116231
rosa_laevigata	RLG00000018249
rosa_multiflora	Rmu_sc0003499.1_g000012 Rmu_sc0004888.1_g000064
rosa_roxburghii	Rroxscaffold_2G00127410
rosa_rugosa	Rorug02G0205000
rosa_samantha	Rh2AG260600 Rh2BG271700 Rh2CG266800 Rh2DG268800
rosa_wichuraiana	Rw2G020430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3785
AccB7I	CCANNNNNTGG	1 cut(s)	191
AccBSI	CCGCTC	2 cut(s)	915, 1463
AccI	GTMKAC	4 cut(s)	201, 937, 2595, 2945
AccII	CGCG	3 cut(s)	1812, 1943, 3665
AccIII	TCCGGA	1 cut(s)	1241
AclWI	GGATC	9 cut(s)	1407, 1420, 1796, 2013, 2058, 2618, 2857, 2870, 3344
AcsI	RAATTY	7 cut(s)	79, 149, 477, 787, 2281, 3050, 3161
AcuI	CTGAAG	5 cut(s)	27, 1440, 1491, 1733, 3300
AfaI	GTAC	7 cut(s)	812, 1043, 1302, 2520, 2766, 2771, 2780
AfeI	AGCGCT	1 cut(s)	2390
AfiI	CCNNNNNNNGG	9 cut(s)	191, 326, 737, 821, 919, 1033, 1241, 2156, 3356
AflIII	ACRYGT	1 cut(s)	2304
AjiI	CACGTC	1 cut(s)	2425
AjnI	CCWGG	6 cut(s)	413, 730, 1408, 1558, 2760, 2955
AjuI	GAANNNNNNNTTGG	6 cut(s)	141, 162, 173, 194, 841, 873
AleI	CACNNNNGTG	1 cut(s)	1758
Alw26I	GTCTC	7 cut(s)	461, 694, 1073, 1517, 1666, 2313, 3064
AlwI	GGATC	9 cut(s)	1407, 1420, 1796, 2013, 2058, 2618, 2857, 2870, 3344
Ama87I	CYCGRG	2 cut(s)	472, 695
Aor13HI	TCCGGA	1 cut(s)	1241
Aor51HI	AGCGCT	1 cut(s)	2390
AoxI	GGCC	5 cut(s)	128, 328, 905, 1406, 1626
ApeKI	GCWGC	8 cut(s)	642, 701, 1200, 2078, 2807, 2828, 2840, 3717
ApoI	RAATTY	7 cut(s)	79, 149, 477, 787, 2281, 3050, 3161
ArsI	GACNNNNNNTTYG	4 cut(s)	2138, 2170, 3266, 3298
Asp700I	GAANNNNTTC	1 cut(s)	236
AspLEI	GCGC	7 cut(s)	413, 797, 1264, 1613, 1814, 2391, 2962
AspS9I	GGNCC	6 cut(s)	182, 314, 728, 1244, 1627, 3039
AsuC2I	CCSGG	1 cut(s)	3575
AvaI	CYCGRG	2 cut(s)	472, 695
AvaII	GGWCC	5 cut(s)	182, 314, 728, 1244, 3039
BaeI	ACNNNNGTAYC	2 cut(s)	1292, 1325
BamHI	GGATCC	2 cut(s)	1412, 2862
BarI	GAAGNNNNNNTAC	3 cut(s)	32, 2511, 2543
BauI	CACGAG	1 cut(s)	2034
BbsI	GAAGAC	6 cut(s)	15, 2427, 2925, 3396, 3645, 3819
BbvCI	CCTCAGC	1 cut(s)	3262
BbvI	GCAGC	8 cut(s)	629, 688, 1187, 2065, 2819, 2840, 2852, 3704
BceAI	ACGGC	2 cut(s)	1712, 3317
BcgI	CGANNNNNNTGC	2 cut(s)	156, 190
BciT130I	CCWGG	6 cut(s)	415, 732, 1410, 1560, 2762, 2957
BclI	TGATCA	1 cut(s)	1873
BcnI	CCSGG	1 cut(s)	3575
BcoDI	GTCTC	7 cut(s)	461, 694, 1073, 1517, 1666, 2313, 3064
BfaI	CTAG	4 cut(s)	1824, 2127, 3384, 3726
BfmI	CTRYAG	4 cut(s)	676, 1583, 2079, 2826
BfoI	RGCGCY	5 cut(s)	414, 798, 1265, 2392, 2963
BglII	AGATCT	4 cut(s)	1313, 2725, 2734, 2855
BisI	GCNGC	8 cut(s)	643, 702, 1201, 2079, 2808, 2829, 2841, 3718
BlpI	GCTNAGC	2 cut(s)	394, 1969
BlsI	GCNGC	8 cut(s)	644, 703, 1202, 2080, 2809, 2830, 2842, 3719
BmcAI	AGTACT	2 cut(s)	1043, 2780
Bme1390I	CCNGG	7 cut(s)	415, 732, 1410, 1560, 2762, 2957, 3575
Bme18I	GGWCC	5 cut(s)	182, 314, 728, 1244, 3039
BmeT110I	CYCGRG	2 cut(s)	472, 695
BmgBI	CACGTC	1 cut(s)	2425
BmgT120I	GGNCC	6 cut(s)	182, 314, 728, 1244, 1627, 3039
BmiI	GGNNCC	5 cut(s)	1414, 1505, 1629, 2456, 2864
BmrFI	CCNGG	7 cut(s)	415, 732, 1410, 1560, 2762, 2957, 3575
BpiI	GAAGAC	6 cut(s)	15, 2427, 2925, 3396, 3645, 3819
BplI	GAGNNNNNCTC	6 cut(s)	2051, 2083, 2053, 2085, 2611, 2643
BpmI	CTGGAG	2 cut(s)	1656, 2729
Bpu10I	CCTNAGC	1 cut(s)	3262
Bpu1102I	GCTNAGC	2 cut(s)	394, 1969
BpuEI	CTTGAG	6 cut(s)	874, 1037, 1077, 1163, 3566, 3577
BpuMI	CCSGG	1 cut(s)	3575
Bsa29I	ATCGAT	1 cut(s)	3703
BsaBI	GATNNNNATC	2 cut(s)	1806, 1983
BsaI	GGTCTC	1 cut(s)	461
BsaJI	CCNNGG	5 cut(s)	414, 471, 694, 766, 3331
BsaWI	WCCGGW	1 cut(s)	1241
Bsc4I	CCNNNNNNNGG	9 cut(s)	191, 326, 737, 821, 919, 1033, 1241, 2156, 3356
Bse118I	RCCGGY	1 cut(s)	126
Bse1I	ACTGG	4 cut(s)	715, 2168, 2746, 2841
Bse3DI	GCAATG	4 cut(s)	1713, 3183, 3687, 3769
Bse8I	GATNNNNATC	2 cut(s)	1806, 1983
BseAI	TCCGGA	1 cut(s)	1241
BseBI	CCWGG	6 cut(s)	415, 732, 1410, 1560, 2762, 2957
BseCI	ATCGAT	1 cut(s)	3703
BseDI	CCNNGG	5 cut(s)	414, 471, 694, 766, 3331
BseGI	GGATG	8 cut(s)	196, 268, 453, 497, 634, 1921, 2248, 3520
BseJI	GATNNNNATC	2 cut(s)	1806, 1983
BseLI	CCNNNNNNNGG	9 cut(s)	191, 326, 737, 821, 919, 1033, 1241, 2156, 3356
BseMI	GCAATG	4 cut(s)	1713, 3183, 3687, 3769
BseMII	CTCAG	9 cut(s)	932, 969, 1686, 1938, 2049, 2551, 3023, 3219, 3276
BseNI	ACTGG	4 cut(s)	715, 2168, 2746, 2841
BseRI	GAGGAG	2 cut(s)	958, 1505
BseXI	GCAGC	8 cut(s)	629, 688, 1187, 2065, 2819, 2840, 2852, 3704
BseYI	CCCAGC	3 cut(s)	623, 1551, 3197
BsgI	GTGCAG	2 cut(s)	2172, 3151
Bsh1236I	CGCG	3 cut(s)	1812, 1943, 3665
BshFI	GGCC	5 cut(s)	130, 330, 907, 1408, 1628
BshVI	ATCGAT	1 cut(s)	3703
BsiHKCI	CYCGRG	2 cut(s)	472, 695
BsiSI	CCGG	3 cut(s)	127, 1242, 3574
BslFI	GGGAC	5 cut(s)	2621, 3214, 3321, 3432, 3509
BslI	CCNNNNNNNGG	9 cut(s)	191, 326, 737, 821, 919, 1033, 1241, 2156, 3356
BsmAI	GTCTC	7 cut(s)	461, 694, 1073, 1517, 1666, 2313, 3064
BsmFI	GGGAC	5 cut(s)	2621, 3214, 3321, 3432, 3509
BsnI	GGCC	5 cut(s)	130, 330, 907, 1408, 1628
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	2 cut(s)	472, 695
Bsp13I	TCCGGA	1 cut(s)	1241
Bsp1407I	TGTACA	1 cut(s)	2518
Bsp1720I	GCTNAGC	2 cut(s)	394, 1969
BspANI	GGCC	5 cut(s)	130, 330, 907, 1408, 1628
BspCNI	CTCAG	9 cut(s)	933, 968, 1685, 1939, 2050, 2550, 3024, 3220, 3275
BspDI	ATCGAT	1 cut(s)	3703
BspEI	TCCGGA	1 cut(s)	1241
BspFNI	CGCG	3 cut(s)	1812, 1943, 3665
BspLI	GGNNCC	5 cut(s)	1414, 1505, 1629, 2456, 2864
BspMAI	CTGCAG	2 cut(s)	2083, 2830
BspPI	GGATC	9 cut(s)	1407, 1420, 1796, 2013, 2058, 2618, 2857, 2870, 3344
BspTNI	GGTCTC	1 cut(s)	461
BsrBI	CCGCTC	2 cut(s)	915, 1463
BsrDI	GCAATG	4 cut(s)	1713, 3183, 3687, 3769
BsrFI	RCCGGY	1 cut(s)	126
BsrGI	TGTACA	1 cut(s)	2518
BsrI	ACTGG	4 cut(s)	715, 2168, 2746, 2841
BssAI	RCCGGY	1 cut(s)	126
BssECI	CCNNGG	5 cut(s)	414, 471, 694, 766, 3331
BssSI	CACGAG	1 cut(s)	2034
BssT1I	CCWWGG	1 cut(s)	766
Bst2BI	CACGAG	1 cut(s)	2034
Bst2UI	CCWGG	6 cut(s)	415, 732, 1410, 1560, 2762, 2957
Bst6I	CTCTTC	4 cut(s)	330, 840, 1709, 3600
BstAPI	GCANNNNNTGC	4 cut(s)	503, 1390, 3194, 3686
BstAUI	TGTACA	1 cut(s)	2518
BstC8I	GCNNGC	7 cut(s)	508, 1266, 1835, 2292, 2404, 2826, 3436
BstEII	GGTNACC	1 cut(s)	122
BstF5I	GGATG	8 cut(s)	196, 268, 453, 497, 634, 1921, 2248, 3520
BstFNI	CGCG	3 cut(s)	1812, 1943, 3665
BstH2I	RGCGCY	5 cut(s)	414, 798, 1265, 2392, 2963
BstHHI	GCGC	7 cut(s)	413, 797, 1264, 1613, 1814, 2391, 2962
BstMAI	GTCTC	7 cut(s)	461, 694, 1073, 1517, 1666, 2313, 3064
BstNI	CCWGG	6 cut(s)	415, 732, 1410, 1560, 2762, 2957
BstNSI	RCATGY	4 cut(s)	1178, 2308, 2512, 2824
BstPI	GGTNACC	1 cut(s)	122
BstSCI	CCNGG	7 cut(s)	413, 730, 1408, 1558, 2760, 2955, 3573
BstSFI	CTRYAG	4 cut(s)	676, 1583, 2079, 2826
BstUI	CGCG	3 cut(s)	1812, 1943, 3665
BstV1I	GCAGC	8 cut(s)	629, 688, 1187, 2065, 2819, 2840, 2852, 3704
BstV2I	GAAGAC	6 cut(s)	15, 2427, 2925, 3396, 3645, 3819
BstX2I	RGATCY	8 cut(s)	1313, 1412, 2063, 2725, 2734, 2855, 2862, 3336
BstXI	CCANNNNNNTGG	1 cut(s)	1559
BstYI	RGATCY	8 cut(s)	1313, 1412, 2063, 2725, 2734, 2855, 2862, 3336
Bsu15I	ATCGAT	1 cut(s)	3703
BsuRI	GGCC	5 cut(s)	130, 330, 907, 1408, 1628
BsuTUI	ATCGAT	1 cut(s)	3703
BtgZI	GCGATG	2 cut(s)	1793, 3567
BtrI	CACGTC	1 cut(s)	2425
BtsCI	GGATG	8 cut(s)	196, 268, 453, 497, 634, 1921, 2248, 3520
BtsIMutI	CAGTG	7 cut(s)	298, 516, 1214, 2110, 3029, 3045, 3225
Cac8I	GCNNGC	7 cut(s)	508, 1266, 1835, 2292, 2404, 2826, 3436
CfoI	GCGC	7 cut(s)	413, 797, 1264, 1613, 1814, 2391, 2962
Cfr10I	RCCGGY	1 cut(s)	126
Cfr13I	GGNCC	6 cut(s)	182, 314, 728, 1244, 1627, 3039
ClaI	ATCGAT	1 cut(s)	3703
Csp6I	GTAC	7 cut(s)	811, 1042, 1301, 2519, 2765, 2770, 2779
CviQI	GTAC	7 cut(s)	811, 1042, 1301, 2519, 2765, 2770, 2779
DraI	TTTAAA	2 cut(s)	1282, 2919
Eam1104I	CTCTTC	4 cut(s)	330, 840, 1709, 3600
EarI	CTCTTC	4 cut(s)	330, 840, 1709, 3600
EciI	GGCGGA	1 cut(s)	1052
Eco130I	CCWWGG	1 cut(s)	766
Eco31I	GGTCTC	1 cut(s)	461
Eco32I	GATATC	2 cut(s)	358, 2715
Eco47I	GGWCC	5 cut(s)	182, 314, 728, 1244, 3039
Eco47III	AGCGCT	1 cut(s)	2390
Eco57I	CTGAAG	5 cut(s)	27, 1440, 1491, 1733, 3300
Eco88I	CYCGRG	2 cut(s)	472, 695
Eco91I	GGTNACC	1 cut(s)	122
EcoO65I	GGTNACC	1 cut(s)	122
EcoRI	GAATTC	3 cut(s)	149, 477, 3161
EcoRII	CCWGG	6 cut(s)	413, 730, 1408, 1558, 2760, 2955
EcoRV	GATATC	2 cut(s)	358, 2715
EcoT14I	CCWWGG	1 cut(s)	766
EcoT22I	ATGCAT	1 cut(s)	2503
ErhI	CCWWGG	1 cut(s)	766
FalI	AAGNNNNNCTT	6 cut(s)	1361, 1393, 2724, 2756, 3177, 3209
FaqI	GGGAC	5 cut(s)	2621, 3214, 3321, 3432, 3509
FauI	CCCGC	3 cut(s)	1826, 3503, 3658
FauNDI	CATATG	3 cut(s)	347, 553, 992
FbaI	TGATCA	1 cut(s)	1873
FblI	GTMKAC	4 cut(s)	201, 937, 2595, 2945
Fnu4HI	GCNGC	8 cut(s)	643, 702, 1201, 2079, 2808, 2829, 2841, 3718
FokI	GGATG	8 cut(s)	203, 275, 460, 504, 641, 1928, 2255, 3527
Fsp4HI	GCNGC	8 cut(s)	643, 702, 1201, 2079, 2808, 2829, 2841, 3718
FspBI	CTAG	4 cut(s)	1824, 2127, 3384, 3726
GlaI	GCGC	7 cut(s)	412, 796, 1263, 1612, 1813, 2390, 2961
GluI	GCNGC	8 cut(s)	643, 702, 1201, 2079, 2808, 2829, 2841, 3718
GsaI	CCCAGC	3 cut(s)	627, 1555, 3201
GsuI	CTGGAG	2 cut(s)	1656, 2729
HaeII	RGCGCY	5 cut(s)	414, 798, 1265, 2392, 2963
HaeIII	GGCC	5 cut(s)	130, 330, 907, 1408, 1628
HapII	CCGG	3 cut(s)	127, 1242, 3574
HhaI	GCGC	7 cut(s)	413, 797, 1264, 1613, 1814, 2391, 2962
Hin6I	GCGC	7 cut(s)	411, 795, 1262, 1611, 1812, 2389, 2960
HinP1I	GCGC	7 cut(s)	411, 795, 1262, 1611, 1812, 2389, 2960
HincII	GTYRAC	2 cut(s)	820, 938
HindII	GTYRAC	2 cut(s)	820, 938
HindIII	AAGCTT	2 cut(s)	245, 2787
HpaII	CCGG	3 cut(s)	127, 1242, 3574
Hpy166II	GTNNAC	7 cut(s)	202, 820, 938, 1183, 2596, 2946, 3698
Hpy188I	TCNGA	8 cut(s)	279, 1088, 1420, 1471, 1696, 2146, 3319, 3747
Hpy8I	GTNNAC	7 cut(s)	202, 820, 938, 1183, 2596, 2946, 3698
HpyAV	CCTTC	9 cut(s)	278, 564, 1334, 1641, 1691, 1898, 1996, 2389, 3324
HpyCH4IV	ACGT	1 cut(s)	2424
HpySE526I	ACGT	1 cut(s)	2424
HspAI	GCGC	7 cut(s)	411, 795, 1262, 1611, 1812, 2389, 2960
Kpn2I	TCCGGA	1 cut(s)	1241
Ksp22I	TGATCA	1 cut(s)	1873
LmnI	GCTCC	4 cut(s)	850, 2281, 3352, 3694
Lsp1109I	GCAGC	8 cut(s)	629, 688, 1187, 2065, 2819, 2840, 2852, 3704
MaeI	CTAG	4 cut(s)	1824, 2127, 3384, 3726
MaeII	ACGT	1 cut(s)	2424
MaeIII	GTNAC	8 cut(s)	122, 1639, 1933, 2375, 2689, 2869, 3291, 3824
MbiI	CCGCTC	2 cut(s)	915, 1463
MflI	RGATCY	8 cut(s)	1313, 1412, 2063, 2725, 2734, 2855, 2862, 3336
MlyI	GAGTC	4 cut(s)	1615, 2939, 3386, 3617
MmeI	TCCRAC	4 cut(s)	292, 853, 872, 1389
Mph1103I	ATGCAT	1 cut(s)	2503
MroI	TCCGGA	1 cut(s)	1241
MroXI	GAANNNNTTC	1 cut(s)	236
MslI	CAYNNNNRTG	3 cut(s)	306, 1737, 1758
MspI	CCGG	3 cut(s)	127, 1242, 3574
MspR9I	CCNGG	7 cut(s)	415, 732, 1410, 1560, 2762, 2957, 3575
MssI	GTTTAAAC	1 cut(s)	1282
MvaI	CCWGG	6 cut(s)	415, 732, 1410, 1560, 2762, 2957
MvnI	CGCG	3 cut(s)	1812, 1943, 3665
NciI	CCSGG	1 cut(s)	3575
NdeI	CATATG	3 cut(s)	347, 553, 992
NlaIV	GGNNCC	5 cut(s)	1414, 1505, 1629, 2456, 2864
NmeAIII	GCCGAG	1 cut(s)	3356
NmuCI	GTSAC	5 cut(s)	122, 2375, 2869, 3291, 3824
NsiI	ATGCAT	1 cut(s)	2503
NspI	RCATGY	4 cut(s)	1178, 2308, 2512, 2824
OliI	CACNNNNGTG	1 cut(s)	1758
PciI	ACATGT	1 cut(s)	2304
PdmI	GAANNNNTTC	1 cut(s)	236
PflMI	CCANNNNNTGG	1 cut(s)	191
PkrI	GCNGC	8 cut(s)	644, 703, 1202, 2080, 2809, 2830, 2842, 3719
PleI	GAGTC	4 cut(s)	1615, 2938, 3386, 3616
PmeI	GTTTAAAC	1 cut(s)	1282
PpsI	GAGTC	4 cut(s)	1615, 2938, 3386, 3616
PscI	ACATGT	1 cut(s)	2304
PsiI	TTATAA	1 cut(s)	3785
Psp6I	CCWGG	6 cut(s)	413, 730, 1408, 1558, 2760, 2955
PspEI	GGTNACC	1 cut(s)	122
PspFI	CCCAGC	3 cut(s)	623, 1551, 3197
PspGI	CCWGG	6 cut(s)	413, 730, 1408, 1558, 2760, 2955
PspN4I	GGNNCC	5 cut(s)	1414, 1505, 1629, 2456, 2864
PspPI	GGNCC	6 cut(s)	182, 314, 728, 1244, 1627, 3039
PstI	CTGCAG	2 cut(s)	2083, 2830
PsuI	RGATCY	8 cut(s)	1313, 1412, 2063, 2725, 2734, 2855, 2862, 3336
RsaI	GTAC	7 cut(s)	812, 1043, 1302, 2520, 2766, 2771, 2780
RsaNI	GTAC	7 cut(s)	811, 1042, 1301, 2519, 2765, 2770, 2779
RseI	CAYNNNNRTG	3 cut(s)	306, 1737, 1758
SalI	GTCGAC	1 cut(s)	936
SatI	GCNGC	8 cut(s)	643, 702, 1201, 2079, 2808, 2829, 2841, 3718
Sau96I	GGNCC	6 cut(s)	182, 314, 728, 1244, 1627, 3039
ScaI	AGTACT	2 cut(s)	1043, 2780
SchI	GAGTC	4 cut(s)	1615, 2939, 3386, 3617
ScrFI	CCNGG	7 cut(s)	415, 732, 1410, 1560, 2762, 2957, 3575
SfcI	CTRYAG	4 cut(s)	676, 1583, 2079, 2826
SinI	GGWCC	5 cut(s)	182, 314, 728, 1244, 3039
SmiMI	CAYNNNNRTG	3 cut(s)	306, 1737, 1758
SmlI	CTYRAG	6 cut(s)	889, 1016, 1092, 1142, 3545, 3556
SmoI	CTYRAG	6 cut(s)	889, 1016, 1092, 1142, 3545, 3556
SspMI	CTAG	4 cut(s)	1824, 2127, 3384, 3726
StyD4I	CCNGG	7 cut(s)	413, 730, 1408, 1558, 2760, 2955, 3573
StyI	CCWWGG	1 cut(s)	766
TaiI	ACGT	1 cut(s)	2427
TatI	WGTACW	4 cut(s)	1041, 2518, 2769, 2778
TscAI	CASTG	7 cut(s)	298, 523, 1221, 2110, 3036, 3052, 3232
TseFI	GTSAC	5 cut(s)	122, 2375, 2869, 3291, 3824
TseI	GCWGC	8 cut(s)	642, 701, 1200, 2078, 2807, 2828, 2840, 3717
Tsp45I	GTSAC	5 cut(s)	122, 2375, 2869, 3291, 3824
TspGWI	ACGGA	5 cut(s)	154, 2619, 2746, 2940, 3681
TspRI	CASTG	7 cut(s)	298, 523, 1221, 2110, 3036, 3052, 3232
Van91I	CCANNNNNTGG	1 cut(s)	191
VpaK11BI	GGWCC	5 cut(s)	182, 314, 728, 1244, 3039
XapI	RAATTY	7 cut(s)	79, 149, 477, 787, 2281, 3050, 3161
XceI	RCATGY	4 cut(s)	1178, 2308, 2512, 2824
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1739
XmiI	GTMKAC	4 cut(s)	201, 937, 2595, 2945
XmnI	GAANNNNTTC	1 cut(s)	236
XspI	CTAG	4 cut(s)	1824, 2127, 3384, 3726
ZrmI	AGTACT	2 cut(s)	1043, 2780
Zsp2I	ATGCAT	1 cut(s)	2503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.