Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002142

Physical Location & Seq

Genomic Coordinates Forward (+)

27891636 .. 27901945

Gene Structure 10310 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G020430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3819 bp

ATGGCGGAAGACAGCCTACGGTTAGGTGGGCCTTCTTCTGGCCTGTTCTTGATATTGAATGGTGAGGACAGCAAAGAGAATTCGTCGAAATCCCATCGTGTTTCATCATGTGATGAGCTCGGTTACCAATCAGTGGAGCGAACCTTGGAATATGTATTTGGTCTCCCTAACAGATCAATCGGTTCGTTGTCTGGGCCGGTTGATGGGAATCTAGTTCGCGGTATTTTAAAGAATGAGTTCTCGAAATTACATGCAAATTCAAGTACTTTGGATGGAGACAGAGATGGCATTTGCATTTCTGGGAATGGCTGCAGACCCTACACCATTGGTCTTGATGAAGTCAGTATCCGGGGTGATATTGGAATCATTAAGCCACCTTTGCTTGTGGAGAGCTTAGCAATGTTCAGCAGTGCTAGGGCTAACGCTTCTGTTTGGAAAGGAAAATGGATGTATGAAGTTATTCTAGAAACTGCAGGGATTCAGCAGCTTGGATGGGCTACTGTTTCTTGCCCTTTCACTGACCATAAGGGTGTAGGTGATGCTGATGATTCATATGCGTTTGATGGGAGGAGGGTTAGAAAATGGAACAAGGATGCTGAGTCATATGGTCAGTCATGGGTTGTTGGTGATGCTATTGGATGCTGCATAGATTTGGACTGTAATGAAATATCATTCTACAGAAATGGTGTCTCTCTTGGTTTGGCATTCCATGGTATTCGTAAGATGGGACCAGGATGTGGATATTATCCTGCAGTTTCTCTCTCACAAGGTGAACGATGTGAATTAAATTTTGGTAGCCGTCCCTTTAAATTTCCGATTGAAGGCTACCAGCCCCTTCAAGCACCTCCTCCTTTAAATTTGTTTGCAACTCAGTTGCTGCGGTGCTTGTCAAGGCTGTTGGGTTTGCACTCTGTGGAGCAGGCCAAACATTCTTCTGTTGAGAAATTGAGACTGAAGAGGTTTGTTTCTCCTGACGAAATTTTTTATCCTGCTTCTCAGGGGATATGTGAGGAACTTTTATCTGTACTTGGAGCTGATGTTTGGAGCATAGAGTATGTTGCCTGGGGTCCCTTTTTGTCATTCATGGTGGAAGTGTTTGGATTGCAGGCGCCACATGATTATTCAGGCTTAGATAGAGTTCTGGATGTCTTTCTAGAATTTAATAGATCACACTTGTTGTTTGAACATATTATAAATGCCCTGGCATGTGGTTGCAAAACGGCCACATTGGTTCTCAAAGAGTGCCCATGGTCAGGGTCATATCCATACCTTGCATTGGCATGTCATATCCTGAGACGGCAGGAACTTATGGTGCTCTGGTGGAAATCACCTGATTTTGAATACCATTTTGAAAGGTTCCTATCACGAAAGGATCCAAATAAGAATGATCTGGAATCCATGATGCCTTCTGTTTGGTGGCCTGGTTCATGTGAGGATGTGTCCTACGAAAGTAGCATGCTGTTGACGACTACAGCTTTATCTGAAGCTGTTAGTAAGATTGAGGAGAAGCATAGGGACCTCTGTCGCTTGGTCATACAGTTCATACCACCTGTCACACCTCCCCAGTTGCCTGGTTCAGTGTTTAGGACATTTCTACAGAACCTTCTACTAAAGAACAGAGGAGCTGACCGCAATCTTCCACCTCCTGGAGTTTCAAGCAATTCTGTTCTTGTTTCTTTGTACACAGTTATACTTCATTTTTTATCTGAGGGGTTTGGAATGGGGGAAATATGCGGTTGGTTGAAGCACAGTGAAAAAGGTCGTGATGTTGGTTTTCTTCATAGGGGTGGTCATCGAACCTTTCCAGTAGGTTTATTTCTTAGAAATGATCCTCATCGAAATGACAATACCAGGCTTGGAGGATCTTTTAGTCATCTCTCAAAATCACACCCTGTAGATGATCAGGAAGCAGAAGATATCCGGTGGGAGGAAGGTTGTATGGATGATGAAGAAACGAGAGTGACTCATCTGAGTACAAGAAAACCATGCTGCTGCTCAAGTTACGACGAGGATTTTACAAGAATCTCAAAGAATCCAATTAGATACACAGCTAAAGGTTCCCGTGCCCATTGCACTCCCATGCCAGAGAGATCTTCCCATGTTACTACAGAATGCAGTGCTGGAAGTTTGAGTGATGATATGGCTGATAAACCTAGTTCTAGCTACCAATCTGAATCCGATTTTAGTTATTGCCCGGTGCAACATTCGAGTTTTGTACCAAGGGAGGGTATTATGTCGTCAGCTACACTCAGAGAAGAAGAACTTCTAGATGTTCTATTGCTGTTGTATCATATAGGTCTTGCGCCAAATTTTAAGCAGGCATCATATTACATGAATCACCAGTTGCAGTCAATTGCCAATCTTGAAGAAACTGATAAAAAAATTCGAGAAGGACCTTGTTTTGAGCAATTGAAGCATTTGAAAGAAGCTCGTAATGTTCATAGAGAAGAGGTTATTGATTCTGTAAGACAATGCGCTTGGTACCGTATCTCCCTATCCTCTAGATGGAAGCAAAGAGGAATGTATGCAACATGCATGTGGACTGTTCAGTTGCTTCTTGTCCTTAGCAAAGTGGATTTGTTGTTCACTTATGTCCCTGAATACTATCTTGAAGCTCTGGTTGACTGCTTTCACGTGTTGCGCAAGTGTGATCCTCCATTTGTCCCTTCATCAATATTTATCAAGCAAGGCCTTGCTTCATTTATCACCTTTGTGGTTACGCATTTCAACGATCCTCGAATATCTAGTGCAGATCTTAGGGATCTGCTTCTCCAATCTATTTCGGTCTTGGTACAATACAAAGAATATTTGGCTGCGTTTGAGAGCAATGAAGCAGCGAAACGGAGGATGCCAAAAGCATTGCTGTCAGCATTTGATAATAGATCCTGGATCCCAGTAACGAACATTCTTCTGCGTTTGTGCAAGGGATCTGGGTTTGGCTCATCAAAGCATGGAGAATCATCGTCATCATCCATCATCTTCCAGAGGTTGTTAGGGCAAGCTTGCATCAGTGATGAAGGGTTATTCTCAGCTTTTCTCAATCGCCTGTTTAACACTCTCAGTTGGACAATGACTGAATTCTCAGTTTCTGTTAGAGAAATGCAAGAAAAGTACCAGGTGTTAGAGTTCCAGCAGAAGAAATGCAGTGTGATATATGATCTGTCATGCAATCTTGCAAGGGTTTTGGAATTCTGTACTCATGAGATACCTCAAGCATTTCTATCTGGGGCTGATACAAACCTTCGGAGGTTGACTGAGCTGATTGTCTTTATTCTGAACCACATCACTTCAGCAGAAGATTCCGAGTTTTTTGACTTGTCTTTGAGACGACATGGTCAATCTTTAGAAAAAGTAAACCGAGGGATGGTATTGGCACCTCTGGTAGGTATTATCTTGAACCTGACAAATGCTAGTGAACAGATGGAATGCAGGGAGCACAATGATGTTGTCAGCATCTTTGCAAGCATGGGGTGCCTTGACAGTTTTAATTGCAGATTTCAGTACCTATTGGATTACAACTGGGCTGGAATTTTAAGAGGAGATGCTTATCTCGGAAAAATTGCGGAGCTAGAGAATTTTCTGAACCTCATCCTTCTTCGTAGCCAATCACAAGAAAATAAGATATCAGGGGGAGAAACTAATGTGAATGATAACATGTGCTGCATTTGTTATGCATGTGAAGCAGATGCACGATTTGCACCTTGTTCCCATAGGTCTTGTTATGGCTGCATAACCAGACATCTTTTGAATTGCCACAGATGCTTCTTCTGCAACGCAACTGTACTAGATGTTGTCAGGATTAGTGAGAAGGCAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0060154 GO:0065007 GO:0070647 GO:0071704 GO:0098586 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPRY Ufd2P_core zf-C3HC4_3

Protein Analysis

1272

Amino Acids

143.23

Weight (kDa)

5.97

Isoelectric Point (pI)

50.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RKP_N	PF19322	1 - 137	1.4e-57	E3 ubiquitin-protein ligase RKP N-terminus
SPRY	PF00622	148 - 266	1.3e-29	SPRY domain
TPR_RNF123	PF25576	1010 - 1202	4.7e-82	RNF123-like, TPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22010 AT2G22010
fragaria_vesca	FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_7g04730 FvH4_7g04730
malus_domestica	MD01G1017400.v1.1 MD15G1333600.v1.1
prunus_persica	Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1
pyrus_communis	pycom01g04820 pycom01g04830 pycom15g29160 pycom15g29170
rosa_chinensis	RchiOBHm_Chr2g0116231
rosa_laevigata	RLG00000018249
rosa_multiflora	Rmu_sc0003499.1_g000012 Rmu_sc0004888.1_g000064
rosa_roxburghii	Rroxscaffold_2G00127410
rosa_rugosa	Rorug02G0205000
rosa_samantha	Rh2AG260600 Rh2BG271700 Rh2CG266800 Rh2DG268800
rosa_wichuraiana	Rw2G020430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1193
AasI	GACNNNNNNGTC	1 cut(s)	3333
Acc16I	TGCGCA	1 cut(s)	2642
Acc65I	GGTACC	1 cut(s)	2481
AccB1I	GGYRCC	4 cut(s)	1108, 2481, 3373, 3471
AccB7I	CCANNNNNTGG	3 cut(s)	133, 737, 1646
AccII	CGCG	1 cut(s)	219
AciI	CCGC	6 cut(s)	5, 219, 880, 1630, 1734, 3563
AcoI	YGGCCR	1 cut(s)	1221
AcuI	CTGAAG	3 cut(s)	974, 1503, 3273
AcvI	CACGTG	1 cut(s)	2635
AcyI	GRCGYC	1 cut(s)	1109
AdeI	CACNNNGTG	2 cut(s)	770, 913
AflIII	ACRYGT	2 cut(s)	2634, 3654
AjnI	CCWGG	9 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2885, 3114
AjuI	GAANNNNNNNTTGG	4 cut(s)	141, 173, 774, 806
AleI	CACNNNNGTG	1 cut(s)	2711
AloI	GAACNNNNNNTCC	2 cut(s)	2567, 2599
Alw21I	GWGCWC	3 cut(s)	120, 1317, 3438
Alw26I	GTCTC	6 cut(s)	167, 270, 694, 943, 1288, 3319
AlwNI	CAGNNNCTG	2 cut(s)	877, 3089
AoxI	GGCC	7 cut(s)	29, 40, 194, 921, 1221, 1418, 2689
Asp700I	GAANNNNTTC	3 cut(s)	236, 459, 2262
Asp718I	GGTACC	1 cut(s)	2481
AspLEI	GCGC	4 cut(s)	1111, 2305, 2477, 2643
AspS9I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
AsuC2I	CCSGG	2 cut(s)	350, 2195
AsuHPI	GGTGA	8 cut(s)	74, 365, 548, 638, 782, 1320, 2330, 2698
AvaII	GGWCC	4 cut(s)	728, 1067, 1516, 2393
BaeGI	GKGCMC	2 cut(s)	1247, 2068
BamHI	GGATCC	2 cut(s)	1372, 2889
BanI	GGYRCC	4 cut(s)	1108, 2481, 3373, 3471
BanII	GRGCYC	1 cut(s)	120
BarI	GAAGNNNNNNTAC	1 cut(s)	32
BbrPI	CACGTG	1 cut(s)	2635
BbsI	GAAGAC	1 cut(s)	15
Bbv12I	GWGCWC	3 cut(s)	120, 1317, 3438
BceAI	ACGGC	3 cut(s)	783, 1236, 1313
BciT130I	CCWGG	9 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2887, 3116
BciVI	GTATCC	1 cut(s)	356
BclI	TGATCA	1 cut(s)	1900
BcnI	CCSGG	2 cut(s)	350, 2195
BcoDI	GTCTC	6 cut(s)	167, 270, 694, 943, 1288, 3319
BfmI	CTRYAG	8 cut(s)	310, 471, 676, 750, 1470, 1595, 1893, 2106
BfoI	RGCGCY	1 cut(s)	1112
BfuI	GTATCC	1 cut(s)	356
BglII	AGATCT	2 cut(s)	2090, 2752
BlpI	GCTNAGC	1 cut(s)	394
BmcAI	AGTACT	1 cut(s)	265
Bme18I	GGWCC	4 cut(s)	728, 1067, 1516, 2393
BmgT120I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
BmrI	ACTGGG	3 cut(s)	1558, 2888, 3529
BmuI	ACTGGG	3 cut(s)	1558, 2888, 3529
BoxI	GACNNNNGTC	1 cut(s)	1521
BpiI	GAAGAC	1 cut(s)	15
BplI	GAGNNNNNCTC	2 cut(s)	1948, 1980
BpmI	CTGGAG	1 cut(s)	1668
Bpu10I	CCTNAGC	1 cut(s)	2564
Bpu1102I	GCTNAGC	1 cut(s)	394
BpuEI	CTTGAG	2 cut(s)	1981, 3195
BpuMI	CCSGG	2 cut(s)	350, 2195
BsaAI	YACGTR	1 cut(s)	2635
BsaBI	GATNNNNATC	3 cut(s)	207, 1833, 3546
BsaHI	GRCGYC	1 cut(s)	1109
BsaI	GGTCTC	1 cut(s)	167
BsaJI	CCNNGG	8 cut(s)	144, 349, 709, 1062, 1200, 1247, 2219, 3358
BsaWI	WCCGGW	1 cut(s)	1920
BsaXI	ACNNNNNCTCC	4 cut(s)	1023, 1053, 2474, 2504
Bse118I	RCCGGY	1 cut(s)	196
Bse1I	ACTGG	5 cut(s)	1564, 1805, 2341, 2894, 3524
Bse3DI	GCAATG	4 cut(s)	405, 2068, 2833, 2858
Bse8I	GATNNNNATC	3 cut(s)	207, 1833, 3546
BseBI	CCWGG	9 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2887, 3116
BseDI	CCNNGG	8 cut(s)	144, 349, 709, 1062, 1200, 1247, 2219, 3358
BseJI	GATNNNNATC	3 cut(s)	207, 1833, 3546
BseMI	GCAATG	4 cut(s)	405, 2068, 2833, 2858
BseNI	ACTGG	5 cut(s)	1564, 1805, 2341, 2894, 3524
BseRI	GAGGAG	5 cut(s)	583, 837, 1517, 1635, 3552
BseSI	GKGCMC	2 cut(s)	1247, 2068
BsgI	GTGCAG	1 cut(s)	2769
Bsh1236I	CGCG	1 cut(s)	219
BshFI	GGCC	7 cut(s)	31, 42, 196, 923, 1223, 1420, 2691
BshNI	GGYRCC	4 cut(s)	1108, 2481, 3373, 3471
BsiHKAI	GWGCWC	3 cut(s)	120, 1317, 3438
BsiSI	CCGG	4 cut(s)	197, 349, 1921, 2195
BslFI	GGGAC	6 cut(s)	741, 786, 1053, 1529, 2579, 2648
BsmAI	GTCTC	6 cut(s)	167, 270, 694, 943, 1288, 3319
BsmBI	CGTCTC	2 cut(s)	1288, 3319
BsmFI	GGGAC	6 cut(s)	741, 786, 1053, 1529, 2579, 2648
BsmI	GAATGC	3 cut(s)	704, 2117, 3431
BsnI	GGCC	7 cut(s)	31, 42, 196, 923, 1223, 1420, 2691
Bso31I	GGTCTC	1 cut(s)	167
Bsp1286I	GDGCHC	5 cut(s)	120, 1247, 1317, 2068, 3438
Bsp1407I	TGTACA	1 cut(s)	1680
Bsp1720I	GCTNAGC	1 cut(s)	394
Bsp19I	CCATGG	2 cut(s)	709, 1247
BspACI	CCGC	6 cut(s)	5, 219, 880, 1630, 1734, 3563
BspANI	GGCC	7 cut(s)	31, 42, 196, 923, 1223, 1420, 2691
BspFNI	CGCG	1 cut(s)	219
BspHI	TCATGA	1 cut(s)	3199
BspMAI	CTGCAG	3 cut(s)	314, 475, 754
BspT107I	GGYRCC	4 cut(s)	1108, 2481, 3373, 3471
BspTNI	GGTCTC	1 cut(s)	167
BsrDI	GCAATG	4 cut(s)	405, 2068, 2833, 2858
BsrFI	RCCGGY	1 cut(s)	196
BsrGI	TGTACA	1 cut(s)	1680
BsrI	ACTGG	5 cut(s)	1564, 1805, 2341, 2894, 3524
BssAI	RCCGGY	1 cut(s)	196
BssECI	CCNNGG	8 cut(s)	144, 349, 709, 1062, 1200, 1247, 2219, 3358
BssNI	GRCGYC	1 cut(s)	1109
BssT1I	CCWWGG	4 cut(s)	144, 709, 1247, 2219
Bst2UI	CCWGG	9 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2887, 3116
Bst6I	CTCTTC	2 cut(s)	950, 2442
BstACI	GRCGYC	1 cut(s)	1109
BstAPI	GCANNNNNTGC	2 cut(s)	3471, 3695
BstAUI	TGTACA	1 cut(s)	1680
BstBAI	YACGTR	1 cut(s)	2635
BstC8I	GCNNGC	7 cut(s)	921, 1107, 1457, 2319, 3000, 3004, 3463
BstDSI	CCRYGG	2 cut(s)	709, 1247
BstEII	GGTNACC	1 cut(s)	122
BstENI	CCTNNNNNAGG	1 cut(s)	3381
BstFNI	CGCG	1 cut(s)	219
BstH2I	RGCGCY	1 cut(s)	1112
BstHHI	GCGC	4 cut(s)	1111, 2305, 2477, 2643
BstMAI	GTCTC	6 cut(s)	167, 270, 694, 943, 1288, 3319
BstMWI	GCNNNNNNNGC	7 cut(s)	379, 2413, 3471, 3680, 3695, 3759, 3768
BstNI	CCWGG	9 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2887, 3116
BstNSI	RCATGY	8 cut(s)	254, 1209, 1284, 1459, 2535, 2539, 3658, 3678
BstPAI	GACNNNNGTC	1 cut(s)	1521
BstPI	GGTNACC	1 cut(s)	122
BstSFI	CTRYAG	8 cut(s)	310, 471, 676, 750, 1470, 1595, 1893, 2106
BstSLI	GKGCMC	2 cut(s)	1247, 2068
BstUI	CGCG	1 cut(s)	219
BstV2I	GAAGAC	1 cut(s)	15
BstX2I	RGATCY	8 cut(s)	1372, 1862, 2090, 2752, 2761, 2882, 2889, 2927
BstXI	CCANNNNNNTGG	1 cut(s)	1571
BstYI	RGATCY	8 cut(s)	1372, 1862, 2090, 2752, 2761, 2882, 2889, 2927
BsuI	GTATCC	1 cut(s)	356
BsuRI	GGCC	7 cut(s)	31, 42, 196, 923, 1223, 1420, 2691
BtgI	CCRYGG	2 cut(s)	709, 1247
BtsI	GCAGTG	3 cut(s)	415, 2122, 3151
BtsIMutI	CAGTG	8 cut(s)	138, 415, 516, 1584, 1756, 2122, 3016, 3151
Cac8I	GCNNGC	7 cut(s)	921, 1107, 1457, 2319, 3000, 3004, 3463
CaiI	CAGNNNCTG	2 cut(s)	877, 3089
CciI	TCATGA	1 cut(s)	3199
CfoI	GCGC	4 cut(s)	1111, 2305, 2477, 2643
Cfr10I	RCCGGY	1 cut(s)	196
Cfr13I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
CsiI	ACCWGGT	1 cut(s)	3114
DinI	GGCGCC	1 cut(s)	1110
DraI	TTTAAA	3 cut(s)	228, 808, 855
DraIII	CACNNNGTG	2 cut(s)	770, 913
DrdI	GACNNNNNNGTC	1 cut(s)	3333
DseDI	GACNNNNNNGTC	1 cut(s)	3333
EaeI	YGGCCR	1 cut(s)	1221
Eam1104I	CTCTTC	2 cut(s)	950, 2442
EarI	CTCTTC	2 cut(s)	950, 2442
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	4 cut(s)	144, 709, 1247, 2219
Eco147I	AGGCCT	1 cut(s)	2691
Eco24I	GRGCYC	1 cut(s)	120
Eco31I	GGTCTC	1 cut(s)	167
Eco32I	GATATC	2 cut(s)	1918, 3624
Eco47I	GGWCC	4 cut(s)	728, 1067, 1516, 2393
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	3 cut(s)	974, 1503, 3273
Eco72I	CACGTG	1 cut(s)	2635
Eco91I	GGTNACC	1 cut(s)	122
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	1 cut(s)	3381
EcoO109I	RGGNCCY	3 cut(s)	1067, 1516, 2393
EcoO65I	GGTNACC	1 cut(s)	122
EcoRI	GAATTC	3 cut(s)	79, 3077, 3188
EcoRII	CCWGG	9 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2885, 3114
EcoRV	GATATC	2 cut(s)	1918, 3624
EcoT14I	CCWWGG	4 cut(s)	144, 709, 1247, 2219
EcoT22I	ATGCAT	2 cut(s)	2537, 3676
EcoT38I	GRGCYC	1 cut(s)	120
EgeI	GGCGCC	1 cut(s)	1110
EheI	GGCGCC	1 cut(s)	1110
ErhI	CCWWGG	4 cut(s)	144, 709, 1247, 2219
Esp3I	CGTCTC	2 cut(s)	1288, 3319
FalI	AAGNNNNNCTT	2 cut(s)	2247, 2279
FaqI	GGGAC	6 cut(s)	741, 786, 1053, 1529, 2579, 2648
FauNDI	CATATG	2 cut(s)	553, 604
FbaI	TGATCA	1 cut(s)	1900
FriOI	GRGCYC	1 cut(s)	120
FspI	TGCGCA	1 cut(s)	2642
GlaI	GCGC	4 cut(s)	1110, 2304, 2476, 2642
GsuI	CTGGAG	1 cut(s)	1668
HaeII	RGCGCY	1 cut(s)	1112
HaeIII	GGCC	7 cut(s)	31, 42, 196, 923, 1223, 1420, 2691
HapII	CCGG	4 cut(s)	197, 349, 1921, 2195
HhaI	GCGC	4 cut(s)	1111, 2305, 2477, 2643
Hin1I	GRCGYC	1 cut(s)	1109
Hin6I	GCGC	4 cut(s)	1109, 2303, 2475, 2641
HinP1I	GCGC	4 cut(s)	1109, 2303, 2475, 2641
HincII	GTYRAC	3 cut(s)	1464, 2623, 3252
HindII	GTYRAC	3 cut(s)	1464, 2623, 3252
HindIII	AAGCTT	1 cut(s)	3000
HpaII	CCGG	4 cut(s)	197, 349, 1921, 2195
HphI	GGTGA	8 cut(s)	74, 365, 548, 638, 782, 1320, 2330, 2698
Hpy166II	GTNNAC	9 cut(s)	773, 1464, 1683, 2541, 2586, 2623, 3252, 3355, 3416
Hpy8I	GTNNAC	9 cut(s)	773, 1464, 1683, 2541, 2586, 2623, 3252, 3355, 3416
Hpy99I	CGWCG	2 cut(s)	88, 2009
HpyCH4IV	ACGT	1 cut(s)	2634
HpyF10VI	GCNNNNNNNGC	7 cut(s)	379, 2413, 3471, 3680, 3695, 3759, 3768
HpySE526I	ACGT	1 cut(s)	2634
Hsp92I	GRCGYC	1 cut(s)	1109
HspAI	GCGC	4 cut(s)	1109, 2303, 2475, 2641
KasI	GGCGCC	1 cut(s)	1108
KflI	GGGWCCC	1 cut(s)	1067
KpnI	GGTACC	1 cut(s)	2485
Ksp22I	TGATCA	1 cut(s)	1900
LmnI	GCTCC	7 cut(s)	136, 916, 1031, 1044, 1622, 3433, 3565
MabI	ACCWGGT	1 cut(s)	3114
MaeII	ACGT	1 cut(s)	2634
MaeIII	GTNAC	7 cut(s)	122, 1552, 1960, 2000, 2101, 2716, 2896
MfeI	CAATTG	2 cut(s)	2352, 2408
MflI	RGATCY	8 cut(s)	1372, 1862, 2090, 2752, 2761, 2882, 2889, 2927
MhlI	GDGCHC	5 cut(s)	120, 1247, 1317, 2068, 3438
Mly113I	GGCGCC	1 cut(s)	1109
MlyI	GAGTC	2 cut(s)	608, 1957
MmeI	TCCRAC	1 cut(s)	3044
Mph1103I	ATGCAT	2 cut(s)	2537, 3676
MroXI	GAANNNNTTC	3 cut(s)	236, 459, 2262
MslI	CAYNNNNRTG	8 cut(s)	528, 1279, 1785, 1839, 2078, 2536, 2711, 3441
MspI	CCGG	4 cut(s)	197, 349, 1921, 2195
MunI	CAATTG	2 cut(s)	2352, 2408
Mva1269I	GAATGC	3 cut(s)	704, 2117, 3431
MvaI	CCWGG	9 cut(s)	732, 1063, 1202, 1422, 1572, 1647, 1852, 2887, 3116
MvnI	CGCG	1 cut(s)	219
MwoI	GCNNNNNNNGC	7 cut(s)	379, 2413, 3471, 3680, 3695, 3759, 3768
NarI	GGCGCC	1 cut(s)	1109
NciI	CCSGG	2 cut(s)	350, 2195
NcoI	CCATGG	2 cut(s)	709, 1247
NdeI	CATATG	2 cut(s)	553, 604
NmuCI	GTSAC	2 cut(s)	1552, 1960
NsbI	TGCGCA	1 cut(s)	2642
NsiI	ATGCAT	2 cut(s)	2537, 3676
NspI	RCATGY	8 cut(s)	254, 1209, 1284, 1459, 2535, 2539, 3658, 3678
OliI	CACNNNNGTG	1 cut(s)	2711
PaeI	GCATGC	1 cut(s)	1459
PagI	TCATGA	1 cut(s)	3199
PceI	AGGCCT	1 cut(s)	2691
PciI	ACATGT	1 cut(s)	3654
PctI	GAATGC	3 cut(s)	704, 2117, 3431
PdmI	GAANNNNTTC	3 cut(s)	236, 459, 2262
PflFI	GACNNNGTC	1 cut(s)	3333
PflMI	CCANNNNNTGG	3 cut(s)	133, 737, 1646
PfoI	TCCNGGA	2 cut(s)	1645, 2885
PleI	GAGTC	2 cut(s)	607, 1957
PluTI	GGCGCC	1 cut(s)	1112
PmaCI	CACGTG	1 cut(s)	2635
PmlI	CACGTG	1 cut(s)	2635
PpsI	GAGTC	2 cut(s)	607, 1957
Ppu21I	YACGTR	1 cut(s)	2635
PpuMI	RGGWCCY	3 cut(s)	1067, 1516, 2393
PscI	ACATGT	1 cut(s)	3654
PshAI	GACNNNNGTC	1 cut(s)	1521
PsiI	TTATAA	1 cut(s)	1193
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	3 cut(s)	1067, 1516, 2393
Psp6I	CCWGG	9 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2885, 3114
PspCI	CACGTG	1 cut(s)	2635
PspEI	GGTNACC	1 cut(s)	122
PspGI	CCWGG	9 cut(s)	730, 1061, 1200, 1420, 1570, 1645, 1850, 2885, 3114
PspPI	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
PspPPI	RGGWCCY	3 cut(s)	1067, 1516, 2393
PstI	CTGCAG	3 cut(s)	314, 475, 754
PstNI	CAGNNNCTG	2 cut(s)	877, 3089
PsuI	RGATCY	8 cut(s)	1372, 1862, 2090, 2752, 2761, 2882, 2889, 2927
PsyI	GACNNNGTC	1 cut(s)	3333
RseI	CAYNNNNRTG	8 cut(s)	528, 1279, 1785, 1839, 2078, 2536, 2711, 3441
SacI	GAGCTC	1 cut(s)	120
Sau96I	GGNCC	6 cut(s)	29, 194, 728, 1067, 1516, 2393
ScaI	AGTACT	1 cut(s)	265
SchI	GAGTC	2 cut(s)	608, 1957
SduI	GDGCHC	5 cut(s)	120, 1247, 1317, 2068, 3438
SexAI	ACCWGGT	1 cut(s)	3114
SfcI	CTRYAG	8 cut(s)	310, 471, 676, 750, 1470, 1595, 1893, 2106
SfoI	GGCGCC	1 cut(s)	1110
SinI	GGWCC	4 cut(s)	728, 1067, 1516, 2393
SmiMI	CAYNNNNRTG	8 cut(s)	528, 1279, 1785, 1839, 2078, 2536, 2711, 3441
SmlI	CTYRAG	2 cut(s)	1996, 3210
SmoI	CTYRAG	2 cut(s)	1996, 3210
SphI	GCATGC	1 cut(s)	1459
SseBI	AGGCCT	1 cut(s)	2691
SsiI	CCGC	6 cut(s)	5, 219, 880, 1630, 1734, 3563
SspDI	GGCGCC	1 cut(s)	1108
SspI	AATATT	2 cut(s)	2676, 2807
SstI	GAGCTC	1 cut(s)	120
StuI	AGGCCT	1 cut(s)	2691
StyI	CCWWGG	4 cut(s)	144, 709, 1247, 2219
TaiI	ACGT	1 cut(s)	2637
TaqI	TCGA	7 cut(s)	86, 242, 1795, 1837, 2207, 2386, 2737
TaqII	GACCGA	1 cut(s)	2773
TatI	WGTACW	6 cut(s)	263, 1024, 1680, 1973, 3194, 3781
TscAI	CASTG	8 cut(s)	138, 415, 523, 1584, 1756, 2122, 3016, 3151
TseFI	GTSAC	2 cut(s)	1552, 1960
Tsp45I	GTSAC	2 cut(s)	1552, 1960
TspGWI	ACGGA	1 cut(s)	2857
TspRI	CASTG	8 cut(s)	138, 415, 523, 1584, 1756, 2122, 3016, 3151
Tth111I	GACNNNGTC	1 cut(s)	3333
Van91I	CCANNNNNTGG	3 cut(s)	133, 737, 1646
VpaK11BI	GGWCC	4 cut(s)	728, 1067, 1516, 2393
XagI	CCTNNNNNAGG	1 cut(s)	3381
XbaI	TCTAGA	4 cut(s)	463, 1153, 2266, 2501
XceI	RCATGY	8 cut(s)	254, 1209, 1284, 1459, 2535, 2539, 3658, 3678
XmnI	GAANNNNTTC	3 cut(s)	236, 459, 2262
ZrmI	AGTACT	1 cut(s)	265
Zsp2I	ATGCAT	2 cut(s)	2537, 3676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.