Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002142

Physical Location & Seq

Genomic Coordinates Reverse (-)

7727785 .. 7738993

Gene Structure 11209 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1017400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3096 bp

ATGGGACCAGGATCTGGATATTATCCTGCAGTTTCTCTTTCTCAAGGCGAAAGATGTGAATTAAATTTTGGTGCTCGACCCTTTAGGTTCCCCATTGAAAGCTACCTCCCCCTTCAGGCACTTCCATCCTTAGTTCCGTTTGCTACTCGGTTGCTGCATTGCTTGTCAAGGCTGTTGGGTCTGCACTCTGTGGAACAGGCAAAGCACTCTTCTGTTCAGAAGTTGAGGAGACTGAAGAGGTTTGTTACACATGAAGAACTTTTTTATCCTGTTTCTCGTGGCGTATGTGAGGAAATTTTCTCAGTACTTGAGGCAGATGTGTGGAGCACAGGGTACATTGCCTGGGGTCCATTTCTGTCATTCATGATGGAAGTGTTCGGGTTGCAGATTCCACATGATTATTCAAGTTTAGATAGAGTTCTTGATGTCTTTCTAGAATTTGAAGGATCACATTTGTTGTTTGAACATTTCATAAATGCCTTGGCTTGTGGTTGCAAAACAGCTCTGCTGGTTCTGGAAGATTGCCCATGTTCAGGGTCATACCCATACCTTGCGGTGGCAATCCACATCCTGCGACGACAAGAGCTTATGGTGCTTTGGTGGAAATCACCTGATTTTGAATTCCTTTTTGAAGGGTTTCTGTCACGGAAGAATCCAAATAAGCATGACCTTGAATCCATGATACCATCTGTTTCGTGGCCTGGTTCAAGTGAGGATGTGTCATATGAAAGTAGCATGTTGTTGACAACTAGAGCTTTATCTGAAGCAGTCAGTAAGATTGAGGAGAAGCATAGGGATCTCTGTCACTTGGTCATACAGTTTATACCACCTGTGACACCTCCCCAGTTTCCTGGTTCAGTGTTCAGAACTGTTTTACAGAACCTTCTACTAAAGAATAGAGGAGCTGACCGCAATCTTCCACCTCCTGGAGTTTCAAGCAATTCTGTTCTTGTTTCTTTGTACACTGTTATACTTCATTTCTTATCTGAAGGGTTTGGCATGGGAGATATTTGCGGCCAGTTGAAAAGCAGTGAAAATGGCCCTGATGTTGGTTTTCTTCATAGGGGTGGTCACCGAAGCTTTCCTGTAGGTCTATTTCTTAGAAATGATCCTCATCGAAATGACAGTTCTCGGCTTGGAGGGTCATTTAGTCATCTTTCAAAATCAAACCGGGTATATGATGAAGAAACAGAACTAATTCGGTGGGAGGAAGGCTGTATGGATGATGAAGAAACCAGAGTGACTCATTCAAGTATAAAAAAGCCGTGTTGTTGCTCATGCTACAATGATGATTTTACAAAAATCTCAAAGTATCCAATTCGATACACAGCCAAAGGTTCTCGTGTCCATTGCAGTTCCATTCCAGAGAGATCTGCCCATGTTGCTACAGAATGCAGTACTGGAAGTTTGAATGATGAAATAGAAGATAAACCGAGCTCTAGTTATCAATCTGAATCTGAGTTTAGTTATCGCTCAGTGCAGCATATGAGGTTTGTACCAAGGGAAAGTAATATGTCTTCAGCTACACTCAGTGAAGAAGAACTTCTAGATGTTTTGCTGTTGTTATATCATATAGGCCTTGCACCAAATTTTAAGCAGGCATCATACTACATGTCTCACCAGTCACAGTCAATATCCCTCATGGAAGAAGCTGATAAACAAATAAGAGAAAAAGCTTGCAGTGAGCAATTAAAGCGTTTGAAAGAAGCTCGTAATGGTTATAGAGAAGAAGTTATTGATTGTGTCAGACAATGTGCTTGGTACCGTATTTCTCTATTTTCTCGATGGAAGCAAAGAGGAATGTATGCAACATGCATGTGGACTGTCCAGTTGCTTTTGGTTCTTACCAAAGTCGATCTGCTGTTCCTTTATATCCCTGAATATTATCTGGAAGCTCTTGTCGATTGCTTTCATGTGTTGCGCAAAAGTGATCCTCCATTTGTACCTTCATCAATATTTATCAAGCAAGGCCTTGCTTCATTTGTCACTTTTGTGGTCACACACTTCAACGATCCTCGGATATCAAGTGCGGATCTTAGGGATCTGCTTCTCCAATCAATCTCAGTCTTGGTACAGTACAAAGAATATCTGTCTGTCTTCGAGAGTAATGAAGCAGCCACCCAGAGAATGCCTAAGGCATTGCTGTCAGCATTTGATAATAGATCCTGGATTCCAGTGACAAACATTCTTCTGCGTTTGTGCAAGGGATCTGGCTTTGGTTCTTCAAAGCAGGGAGAATCGTCGTCTTCATCCGTTGTCTTCCAGAGATTGTTAGGTGAAACATGTATCAACGATCAAGAATTGTTCTCAGCTTTTCTCAATCGCCTATTTAACACTCTCAGCTGGACAATGACTGAATTCTCAGTTTCTGTTAGAGAAATGCAAAAGAAGTACCAGGTATTAGAGTTCCAGCAAAAGAAATGCGGTGTCATATTTGATCTTTCATGCAATTTGACAAAGCTTTTGGAGTTCTGTACCCATGCGATACCTCAAGCATTTCTGTCTGGAGCTGAAACAAACCTTCGAAGGTTGATGGAGTTGGTTGTCTTTATTCTGAACCACATAACTTCAGCAGAAGATGCTGAATTTTTTGACTTGTCACTGAGACGACTTGGCCAATCTATAGAAAAAATAAACCGAGGGATGATATTGGCACCCCTTGTAGGCATTATCTTGAATTTGTTAAATGCCAGCGAACATATGGAATGCAGGGAGCACAATGATGTTGTGAGCATCTTTGCAAGCATGGGTTGCCTTGACAGTTTTCATTGCAGATTCCAGTACCTAGTGGATTACAACTGGGATGGAACCTGTAGAGGTGATGCGTATCTTGTAAAAATTGCCCAGCTAGAGAATTTTCTGAGCCTTCTCAGCCAGAGCCAATCACAAGAAAATGCAATATGCAGGGGTGAAAAGGATGGGGATGATGGCATGTGCTGCATCTGTTACGCCCGTGAAGCAGATGCCCAGTTTTCACCTTGTTCGCACAGGTCTTGTTATGGTTGCATAACCAGGCATCTTTTGAATTGCCACAGATGTTTTTTTTGCAATGCAACAGTTGTGGACGTTGTCCTGATCAGTGAGAAGACGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0060154 GO:0065007 GO:0070647 GO:0071704 GO:0098586 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPRY Ufd2P_core zf-C3HC4_3

Protein Analysis

1032

Amino Acids

117.05

Weight (kDa)

6.04

Isoelectric Point (pI)

50.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ufd2P_core	PF10408	521 - 718	4.2e-06	Ubiquitin elongating factor core
TPR_RNF123	PF25576	770 - 962	4.2e-80	RNF123-like, TPR repeat
zf-C3HC4_3	PF13920	976 - 1021	3.6e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22010 AT2G22010
fragaria_vesca	FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_7g04730 FvH4_7g04730
malus_domestica	MD01G1017400.v1.1 MD15G1333600.v1.1
prunus_persica	Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1
pyrus_communis	pycom01g04820 pycom01g04830 pycom15g29160 pycom15g29170
rosa_chinensis	RchiOBHm_Chr2g0116231
rosa_laevigata	RLG00000018249
rosa_multiflora	Rmu_sc0003499.1_g000012 Rmu_sc0004888.1_g000064
rosa_roxburghii	Rroxscaffold_2G00127410
rosa_rugosa	Rorug02G0205000
rosa_samantha	Rh2AG260600 Rh2BG271700 Rh2CG266800 Rh2DG268800
rosa_wichuraiana	Rw2G020430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1922
Acc65I	GGTACC	1 cut(s)	1761
AccB1I	GGYRCC	2 cut(s)	1761, 2653
AccB7I	CCANNNNNTGG	2 cut(s)	14, 926
AciI	CCGC	5 cut(s)	554, 910, 1014, 2030, 2424
AcoI	YGGCCR	2 cut(s)	1015, 2614
AcsI	RAATTY	9 cut(s)	64, 294, 437, 620, 1588, 2357, 2585, 2677, 2854
AcuI	CTGAAG	6 cut(s)	98, 254, 783, 1008, 1503, 2553
AdeI	CACNNNGTG	2 cut(s)	190, 1532
AfiI	CCNNNNNNNGG	7 cut(s)	14, 115, 533, 556, 926, 1049, 2663
AflIII	ACRYGT	2 cut(s)	1611, 2282
AjnI	CCWGG	8 cut(s)	7, 341, 700, 850, 925, 2165, 2394, 3011
AjuI	GAANNNNNNNTTGG	2 cut(s)	51, 83
AleI	CACNNNNGTG	1 cut(s)	1991
Alw21I	GWGCWC	4 cut(s)	76, 329, 1439, 2718
Alw26I	GTCTC	3 cut(s)	223, 1620, 2599
AlwNI	CAGNNNCTG	2 cut(s)	14, 2369
AoxI	GGCC	6 cut(s)	698, 1015, 1039, 1576, 1969, 2614
ApeKI	GCWGC	4 cut(s)	154, 1480, 2114, 2937
ApoI	RAATTY	9 cut(s)	64, 294, 437, 620, 1588, 2357, 2585, 2677, 2854
ArsI	GACNNNNNNTTYG	2 cut(s)	2446, 2478
Asp700I	GAANNNNTTC	3 cut(s)	636, 1197, 1542
Asp718I	GGTACC	1 cut(s)	1761
AspLEI	GCGC	1 cut(s)	1923
AspS9I	GGNCC	3 cut(s)	5, 347, 1040
AsuC2I	CCSGG	1 cut(s)	1172
AsuHPI	GGTGA	7 cut(s)	600, 1064, 1610, 2288, 2831, 2921, 2967
AsuII	TTCGAA	1 cut(s)	2524
AvaII	GGWCC	2 cut(s)	5, 347
AxyI	CCTNAGG	1 cut(s)	2133
BalI	TGGCCA	1 cut(s)	2616
BanI	GGYRCC	2 cut(s)	1761, 2653
BanII	GRGCYC	1 cut(s)	1439
BarI	GAAGNNNNNNTAC	2 cut(s)	1501, 1533
BauI	CACGAG	2 cut(s)	276, 1341
BbsI	GAAGAC	5 cut(s)	1509, 2089, 2238, 2251, 3092
Bbv12I	GWGCWC	4 cut(s)	76, 329, 1439, 2718
BbvI	GCAGC	4 cut(s)	141, 1492, 2126, 2924
BccI	CCATC	8 cut(s)	133, 361, 694, 1779, 2527, 2798, 2912, 2921
BceAI	ACGGC	1 cut(s)	1249
BciT130I	CCWGG	8 cut(s)	9, 343, 702, 852, 927, 2167, 2396, 3013
BciVI	GTATCC	1 cut(s)	1323
BclI	TGATCA	1 cut(s)	3075
BcnI	CCSGG	1 cut(s)	1172
BcoDI	GTCTC	3 cut(s)	223, 1620, 2599
BfaI	CTAG	6 cut(s)	434, 750, 1440, 1547, 2786, 2849
BfmI	CTRYAG	5 cut(s)	27, 1086, 1386, 2622, 2812
BfuI	GTATCC	1 cut(s)	1323
BglII	AGATCT	1 cut(s)	1370
BisI	GCNGC	5 cut(s)	155, 1015, 1481, 2115, 2938
BlsI	GCNGC	5 cut(s)	156, 1016, 1482, 2116, 2939
BmcAI	AGTACT	2 cut(s)	306, 1399
Bme1390I	CCNGG	9 cut(s)	9, 343, 702, 852, 927, 1172, 2167, 2396, 3013
Bme18I	GGWCC	2 cut(s)	5, 347
BmgT120I	GGNCC	3 cut(s)	5, 347, 1040
BmiI	GGNNCC	6 cut(s)	6, 89, 348, 1763, 2655, 2809
BmrFI	CCNGG	9 cut(s)	9, 343, 702, 852, 927, 1172, 2167, 2396, 3013
BmrI	ACTGGG	3 cut(s)	838, 2809, 2962
BmsI	GCATC	7 cut(s)	1610, 2569, 2742, 2812, 2949, 2953, 3025
BmuI	ACTGGG	3 cut(s)	838, 2809, 2962
BpiI	GAAGAC	5 cut(s)	1509, 2089, 2238, 2251, 3092
BpmI	CTGGAG	2 cut(s)	948, 2526
Bpu14I	TTCGAA	1 cut(s)	2524
BpuEI	CTTGAG	3 cut(s)	27, 329, 2475
BpuMI	CCSGG	1 cut(s)	1172
BsaBI	GATNNNNATC	2 cut(s)	1113, 2826
BsaJI	CCNNGG	5 cut(s)	342, 480, 1499, 2015, 2638
Bsc4I	CCNNNNNNNGG	7 cut(s)	14, 115, 533, 556, 926, 1049, 2663
Bse1I	ACTGG	9 cut(s)	844, 1018, 1405, 1621, 1828, 2174, 2779, 2804, 2968
Bse21I	CCTNAGG	1 cut(s)	2133
Bse3DI	GCAATG	6 cut(s)	157, 336, 1348, 2138, 2767, 3055
Bse8I	GATNNNNATC	2 cut(s)	1113, 2826
BseBI	CCWGG	8 cut(s)	9, 343, 702, 852, 927, 2167, 2396, 3013
BseDI	CCNNGG	5 cut(s)	342, 480, 1499, 2015, 2638
BseGI	GGATG	9 cut(s)	125, 567, 721, 1228, 2249, 2649, 2809, 2923, 2929
BseJI	GATNNNNATC	2 cut(s)	1113, 2826
BseLI	CCNNNNNNNGG	7 cut(s)	14, 115, 533, 556, 926, 1049, 2663
BseMI	GCAATG	6 cut(s)	157, 336, 1348, 2138, 2767, 3055
BseNI	ACTGG	9 cut(s)	844, 1018, 1405, 1621, 1828, 2174, 2779, 2804, 2968
BseRI	GAGGAG	3 cut(s)	241, 797, 915
BseXI	GCAGC	4 cut(s)	141, 1492, 2126, 2924
BseYI	CCCAGC	1 cut(s)	2844
BsgI	GTGCAG	2 cut(s)	167, 1499
BshFI	GGCC	6 cut(s)	700, 1017, 1041, 1578, 1971, 2616
BshNI	GGYRCC	2 cut(s)	1761, 2653
BsiHKAI	GWGCWC	4 cut(s)	76, 329, 1439, 2718
BsiSI	CCGG	1 cut(s)	1171
BslFI	GGGAC	1 cut(s)	18
BslI	CCNNNNNNNGG	7 cut(s)	14, 115, 533, 556, 926, 1049, 2663
BsmAI	GTCTC	3 cut(s)	223, 1620, 2599
BsmBI	CGTCTC	1 cut(s)	2599
BsmFI	GGGAC	1 cut(s)	18
BsmI	GAATGC	3 cut(s)	1397, 2133, 2711
BsnI	GGCC	6 cut(s)	700, 1017, 1041, 1578, 1971, 2616
Bsp119I	TTCGAA	1 cut(s)	2524
Bsp1286I	GDGCHC	4 cut(s)	76, 329, 1439, 2718
Bsp1407I	TGTACA	1 cut(s)	960
BspACI	CCGC	5 cut(s)	554, 910, 1014, 2030, 2424
BspANI	GGCC	6 cut(s)	700, 1017, 1041, 1578, 1971, 2616
BspHI	TCATGA	1 cut(s)	363
BspLI	GGNNCC	6 cut(s)	6, 89, 348, 1763, 2655, 2809
BspMAI	CTGCAG	1 cut(s)	31
BspT104I	TTCGAA	1 cut(s)	2524
BspT107I	GGYRCC	2 cut(s)	1761, 2653
BsrDI	GCAATG	6 cut(s)	157, 336, 1348, 2138, 2767, 3055
BsrGI	TGTACA	1 cut(s)	960
BsrI	ACTGG	9 cut(s)	844, 1018, 1405, 1621, 1828, 2174, 2779, 2804, 2968
BssECI	CCNNGG	5 cut(s)	342, 480, 1499, 2015, 2638
BssSI	CACGAG	2 cut(s)	276, 1341
BssT1I	CCWWGG	2 cut(s)	480, 1499
Bst2BI	CACGAG	2 cut(s)	276, 1341
Bst2UI	CCWGG	8 cut(s)	9, 343, 702, 852, 927, 2167, 2396, 3013
Bst6I	CTCTTC	2 cut(s)	214, 230
BstAPI	GCANNNNNTGC	3 cut(s)	2579, 2751, 2937
BstAUI	TGTACA	1 cut(s)	960
BstBI	TTCGAA	1 cut(s)	2524
BstC8I	GCNNGC	4 cut(s)	1599, 1678, 2692, 2743
BstEII	GGTNACC	1 cut(s)	1070
BstF5I	GGATG	9 cut(s)	125, 567, 721, 1228, 2249, 2649, 2809, 2923, 2929
BstHHI	GCGC	1 cut(s)	1923
BstMAI	GTCTC	3 cut(s)	223, 1620, 2599
BstMWI	GCNNNNNNNGC	8 cut(s)	592, 1382, 1693, 2579, 2751, 2871, 2937, 2957
BstNI	CCWGG	8 cut(s)	9, 343, 702, 852, 927, 2167, 2396, 3013
BstNSI	RCATGY	6 cut(s)	739, 1615, 1815, 1819, 2286, 2935
BstPI	GGTNACC	1 cut(s)	1070
BstSCI	CCNGG	9 cut(s)	7, 341, 700, 850, 925, 1170, 2165, 2394, 3011
BstSFI	CTRYAG	5 cut(s)	27, 1086, 1386, 2622, 2812
BstV1I	GCAGC	4 cut(s)	141, 1492, 2126, 2924
BstV2I	GAAGAC	5 cut(s)	1509, 2089, 2238, 2251, 3092
BstX2I	RGATCY	7 cut(s)	11, 796, 1370, 2032, 2041, 2162, 2207
BstXI	CCANNNNNNTGG	1 cut(s)	851
BstYI	RGATCY	7 cut(s)	11, 796, 1370, 2032, 2041, 2162, 2207
Bsu36I	CCTNAGG	1 cut(s)	2133
BsuI	GTATCC	1 cut(s)	1323
BsuRI	GGCC	6 cut(s)	700, 1017, 1041, 1578, 1971, 2616
BtsCI	GGATG	9 cut(s)	125, 567, 721, 1228, 2249, 2649, 2809, 2923, 2929
BtsI	GCAGTG	2 cut(s)	1036, 1687
BtsIMutI	CAGTG	9 cut(s)	864, 963, 1036, 1482, 1537, 1687, 2181, 2600, 3085
Cac8I	GCNNGC	4 cut(s)	1599, 1678, 2692, 2743
CaiI	CAGNNNCTG	2 cut(s)	14, 2369
CciI	TCATGA	1 cut(s)	363
CfoI	GCGC	1 cut(s)	1923
Cfr13I	GGNCC	3 cut(s)	5, 347, 1040
CsiI	ACCWGGT	1 cut(s)	2394
CspCI	CAANNNNNGTGG	2 cut(s)	3042, 3077
DraIII	CACNNNGTG	2 cut(s)	190, 1532
EaeI	YGGCCR	2 cut(s)	1015, 2614
Eam1104I	CTCTTC	2 cut(s)	214, 230
EarI	CTCTTC	2 cut(s)	214, 230
Ecl136II	GAGCTC	1 cut(s)	1437
Eco130I	CCWWGG	2 cut(s)	480, 1499
Eco147I	AGGCCT	2 cut(s)	1578, 1971
Eco24I	GRGCYC	1 cut(s)	1439
Eco32I	GATATC	1 cut(s)	2022
Eco47I	GGWCC	2 cut(s)	5, 347
Eco53kI	GAGCTC	1 cut(s)	1437
Eco57I	CTGAAG	6 cut(s)	98, 254, 783, 1008, 1503, 2553
Eco81I	CCTNAGG	1 cut(s)	2133
Eco91I	GGTNACC	1 cut(s)	1070
EcoICRI	GAGCTC	1 cut(s)	1437
EcoO65I	GGTNACC	1 cut(s)	1070
EcoRI	GAATTC	2 cut(s)	620, 2357
EcoRII	CCWGG	8 cut(s)	7, 341, 700, 850, 925, 2165, 2394, 3011
EcoRV	GATATC	1 cut(s)	2022
EcoT14I	CCWWGG	2 cut(s)	480, 1499
EcoT22I	ATGCAT	1 cut(s)	1817
EcoT38I	GRGCYC	1 cut(s)	1439
ErhI	CCWWGG	2 cut(s)	480, 1499
Esp3I	CGTCTC	1 cut(s)	2599
FalI	AAGNNNNNCTT	2 cut(s)	1527, 1559
FaqI	GGGAC	1 cut(s)	18
FauNDI	CATATG	3 cut(s)	724, 1485, 2700
FbaI	TGATCA	1 cut(s)	3075
Fnu4HI	GCNGC	5 cut(s)	155, 1015, 1481, 2115, 2938
FokI	GGATG	9 cut(s)	112, 554, 728, 1235, 2236, 2656, 2816, 2930, 2936
FriOI	GRGCYC	1 cut(s)	1439
Fsp4HI	GCNGC	5 cut(s)	155, 1015, 1481, 2115, 2938
FspBI	CTAG	6 cut(s)	434, 750, 1440, 1547, 2786, 2849
FspI	TGCGCA	1 cut(s)	1922
GlaI	GCGC	1 cut(s)	1922
GluI	GCNGC	5 cut(s)	155, 1015, 1481, 2115, 2938
GsaI	CCCAGC	1 cut(s)	2848
GsuI	CTGGAG	2 cut(s)	948, 2526
HaeIII	GGCC	6 cut(s)	700, 1017, 1041, 1578, 1971, 2616
HapII	CCGG	1 cut(s)	1171
HhaI	GCGC	1 cut(s)	1923
Hin6I	GCGC	1 cut(s)	1921
HinP1I	GCGC	1 cut(s)	1921
HincII	GTYRAC	1 cut(s)	744
HindII	GTYRAC	1 cut(s)	744
HindIII	AAGCTT	3 cut(s)	1078, 1674, 2459
HinfI	GANTC	8 cut(s)	388, 652, 674, 1243, 1454, 2170, 2237, 2775
HpaII	CCGG	1 cut(s)	1171
HphI	GGTGA	7 cut(s)	600, 1064, 1610, 2288, 2831, 2921, 2967
Hpy166II	GTNNAC	4 cut(s)	744, 963, 1821, 3064
Hpy8I	GTNNAC	4 cut(s)	744, 963, 1821, 3064
Hpy99I	CGWCG	2 cut(s)	579, 2245
HpyCH4IV	ACGT	1 cut(s)	3066
HpyF10VI	GCNNNNNNNGC	8 cut(s)	592, 1382, 1693, 2579, 2751, 2871, 2937, 2957
HpySE526I	ACGT	1 cut(s)	3066
HspAI	GCGC	1 cut(s)	1921
KpnI	GGTACC	1 cut(s)	1765
Ksp22I	TGATCA	1 cut(s)	3075
LmnI	GCTCC	4 cut(s)	324, 902, 2507, 2713
Lsp1109I	GCAGC	4 cut(s)	141, 1492, 2126, 2924
LweI	GCATC	7 cut(s)	1610, 2569, 2742, 2812, 2949, 2953, 3025
MabI	ACCWGGT	1 cut(s)	2394
MaeI	CTAG	6 cut(s)	434, 750, 1440, 1547, 2786, 2849
MaeII	ACGT	1 cut(s)	3066
MflI	RGATCY	7 cut(s)	11, 796, 1370, 2032, 2041, 2162, 2207
MhlI	GDGCHC	4 cut(s)	76, 329, 1439, 2718
MlsI	TGGCCA	1 cut(s)	2616
MluNI	TGGCCA	1 cut(s)	2616
MlyI	GAGTC	1 cut(s)	1237
Mox20I	TGGCCA	1 cut(s)	2616
Mph1103I	ATGCAT	1 cut(s)	1817
MroXI	GAANNNNTTC	3 cut(s)	636, 1197, 1542
MscI	TGGCCA	1 cut(s)	2616
MseI	TTAA	5 cut(s)	62, 1593, 1691, 2331, 2684
MslI	CAYNNNNRTG	5 cut(s)	1065, 1119, 1816, 1991, 2721
Msp20I	TGGCCA	1 cut(s)	2616
MspA1I	CMGCKG	1 cut(s)	2343
MspI	CCGG	1 cut(s)	1171
MspR9I	CCNGG	9 cut(s)	9, 343, 702, 852, 927, 1172, 2167, 2396, 3013
Mva1269I	GAATGC	3 cut(s)	1397, 2133, 2711
MvaI	CCWGG	8 cut(s)	9, 343, 702, 852, 927, 2167, 2396, 3013
MwoI	GCNNNNNNNGC	8 cut(s)	592, 1382, 1693, 2579, 2751, 2871, 2937, 2957
NciI	CCSGG	1 cut(s)	1172
NdeI	CATATG	3 cut(s)	724, 1485, 2700
NlaIV	GGNNCC	6 cut(s)	6, 89, 348, 1763, 2655, 2809
NmeAIII	GCCGAG	1 cut(s)	1111
NsbI	TGCGCA	1 cut(s)	1922
NsiI	ATGCAT	1 cut(s)	1817
NspI	RCATGY	6 cut(s)	739, 1615, 1815, 1819, 2286, 2935
NspV	TTCGAA	1 cut(s)	2524
OliI	CACNNNNGTG	1 cut(s)	1991
PagI	TCATGA	1 cut(s)	363
PceI	AGGCCT	2 cut(s)	1578, 1971
PciI	ACATGT	2 cut(s)	1611, 2282
PctI	GAATGC	3 cut(s)	1397, 2133, 2711
PdmI	GAANNNNTTC	3 cut(s)	636, 1197, 1542
PfeI	GAWTC	7 cut(s)	388, 652, 674, 1454, 2170, 2237, 2775
PflFI	GACNNNGTC	1 cut(s)	3068
PflMI	CCANNNNNTGG	2 cut(s)	14, 926
PfoI	TCCNGGA	2 cut(s)	925, 2165
PkrI	GCNGC	5 cut(s)	156, 1016, 1482, 2116, 2939
PleI	GAGTC	1 cut(s)	1237
PpsI	GAGTC	1 cut(s)	1237
PscI	ACATGT	2 cut(s)	1611, 2282
Psp124BI	GAGCTC	1 cut(s)	1439
Psp6I	CCWGG	8 cut(s)	7, 341, 700, 850, 925, 2165, 2394, 3011
PspEI	GGTNACC	1 cut(s)	1070
PspFI	CCCAGC	1 cut(s)	2844
PspGI	CCWGG	8 cut(s)	7, 341, 700, 850, 925, 2165, 2394, 3011
PspN4I	GGNNCC	6 cut(s)	6, 89, 348, 1763, 2655, 2809
PspPI	GGNCC	3 cut(s)	5, 347, 1040
PstI	CTGCAG	1 cut(s)	31
PstNI	CAGNNNCTG	2 cut(s)	14, 2369
PsuI	RGATCY	7 cut(s)	11, 796, 1370, 2032, 2041, 2162, 2207
PsyI	GACNNNGTC	1 cut(s)	3068
PvuII	CAGCTG	1 cut(s)	2343
RseI	CAYNNNNRTG	5 cut(s)	1065, 1119, 1816, 1991, 2721
SacI	GAGCTC	1 cut(s)	1439
SaqAI	TTAA	5 cut(s)	62, 1593, 1691, 2331, 2684
SatI	GCNGC	5 cut(s)	155, 1015, 1481, 2115, 2938
Sau96I	GGNCC	3 cut(s)	5, 347, 1040
ScaI	AGTACT	2 cut(s)	306, 1399
SchI	GAGTC	1 cut(s)	1237
ScrFI	CCNGG	9 cut(s)	9, 343, 702, 852, 927, 1172, 2167, 2396, 3013
SduI	GDGCHC	4 cut(s)	76, 329, 1439, 2718
SexAI	ACCWGGT	1 cut(s)	2394
SfaNI	GCATC	7 cut(s)	1610, 2569, 2742, 2812, 2949, 2953, 3025
SfcI	CTRYAG	5 cut(s)	27, 1086, 1386, 2622, 2812
SfuI	TTCGAA	1 cut(s)	2524
SinI	GGWCC	2 cut(s)	5, 347
SmiMI	CAYNNNNRTG	5 cut(s)	1065, 1119, 1816, 1991, 2721
SmlI	CTYRAG	3 cut(s)	42, 308, 2490
SmoI	CTYRAG	3 cut(s)	42, 308, 2490
SseBI	AGGCCT	2 cut(s)	1578, 1971
SsiI	CCGC	5 cut(s)	554, 910, 1014, 2030, 2424
SspI	AATATT	2 cut(s)	1883, 1956
SspMI	CTAG	6 cut(s)	434, 750, 1440, 1547, 2786, 2849
SstI	GAGCTC	1 cut(s)	1439
StuI	AGGCCT	2 cut(s)	1578, 1971
StyD4I	CCNGG	9 cut(s)	7, 341, 700, 850, 925, 1170, 2165, 2394, 3011
StyI	CCWWGG	2 cut(s)	480, 1499
TaiI	ACGT	1 cut(s)	3069
TaqI	TCGA	8 cut(s)	76, 1117, 1321, 1783, 1854, 1902, 2100, 2524
TatI	WGTACW	4 cut(s)	304, 960, 1397, 2076
TauI	GCSGC	1 cut(s)	1017
TfiI	GAWTC	7 cut(s)	388, 652, 674, 1454, 2170, 2237, 2775
Tru1I	TTAA	5 cut(s)	62, 1593, 1691, 2331, 2684
Tru9I	TTAA	5 cut(s)	62, 1593, 1691, 2331, 2684
TscAI	CASTG	9 cut(s)	864, 970, 1036, 1482, 1537, 1687, 2181, 2607, 3085
TseI	GCWGC	4 cut(s)	154, 1480, 2114, 2937
TspGWI	ACGGA	3 cut(s)	126, 661, 2242
TspRI	CASTG	9 cut(s)	864, 970, 1036, 1482, 1537, 1687, 2181, 2607, 3085
Tth111I	GACNNNGTC	1 cut(s)	3068
Van91I	CCANNNNNTGG	2 cut(s)	14, 926
VpaK11BI	GGWCC	2 cut(s)	5, 347
XapI	RAATTY	9 cut(s)	64, 294, 437, 620, 1588, 2357, 2585, 2677, 2854
XbaI	TCTAGA	2 cut(s)	433, 1546
XceI	RCATGY	6 cut(s)	739, 1615, 1815, 1819, 2286, 2935
XcmI	CCANNNNNNNNNTGG	2 cut(s)	693, 2698
XmnI	GAANNNNTTC	3 cut(s)	636, 1197, 1542
XspI	CTAG	6 cut(s)	434, 750, 1440, 1547, 2786, 2849
ZrmI	AGTACT	2 cut(s)	306, 1399
Zsp2I	ATGCAT	1 cut(s)	1817

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.