Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0002142

Physical Location & Seq

Genomic Coordinates Forward (+)

14093372 .. 14103645

Gene Structure 10274 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g22150.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3828 bp

ATGGCGGAAGACGGCCTACGGTTAGGTGGGCTTTCTTCTGGTCTGGCCTTGATATTGAATGGTGAGAGCAGCAAAGAGAATTCGTCGAAAACTCATCTTGTTTCGTCGTGTGATGAGATTGGATACCAATCAGTGGAGAGAACTTTAGAATATGTGTTTGGTTTCCCTAATAGATCGATTGGTCCTGTGTCTGGGCCGGTTGATGGGAATCTAGTTCGTGGTATTTTGAAGAATGAGTTCTCGAAATTACATGCAAATTCAAGTGTTTTGGATGGAGATGGGTATAGGGACGGTATTTGTATTTATGGGAGTGGCTGCAGACCCGACACCGTTGGTCTTGATGAAATTAGTATCCGGGGTGATATTAGAATCATTAAGCCGCCGTTGCTTGTAGAGAGCTTAGCAATGTTCAGCAGTGCTAGGGCTAACGCTTCTGTGTGGAAAGGAAAATGGATGTATGAGGTTATTCTGGAAACTGCGGGTATTCAGCAGATTGGATGGGCTACTGTTTCTTGCCCATTCACTGACCATAAGGGTGTAGGTGATGCTGAAGATTCGTATGCGTTTGATGGTAGGAGGGTTAGGAAATGGAACAGGGATGCTGAGTCATATGGTCAGACATGGGTTGTTGGTGATGTTATTGGATGTTGCATAGATTTGGATTGTAATGAGATATCATTTTACAGAAATGGTGTCTCTCTTGGTTTGGCATTCAATGGTATTCGTAAAATGGGAGTGGGATGTGGATATTATCCTGCTGTATCTCTCTCACAAGGTGAACGATGCGAATTAAATTTTGGTGGACGTCCCTTTAAATTTCCCATTGAAGGCTACCACCCCCTTCAAGCCCCTCCGTCCTTAAACTCATTTGCAACTCAATTGCTGCGGTGCTTGTCAAGGCTGTTGGGTTTGCACTCTGTGGAGCGGGCAAAGCATTCTTCTGTTGAGAAATTGAGACTGAAAAGGTTTGTTTCTCCAGATGAAATTTTCTATCCTATTTCTCATGGGATATGCGAGGAGCTTTTCTCTGTACTTGGAGCAGATGTTTGGAGCATAGAGTATGTTGCCTGGGGTCCGTTTTTGTCGTTCATGGTGGAAGCGTTTGGGTTGCAGGCGCCACATGATTATTCAGGCTTGGATAGAGTTCTGGATGTCTTTCTAGAATTTAATAGATCACACTTGTTGTTTGAACATATTATAAATGCCCTGGCATGTGGTTGCAAAACAGCTCCATTAGTTCTCAAAGAGTGCCCATGTTCAGGATCATATCCATACCTCGCATTGGCATGTCATATCCTGAGACGACAAGAACTTATGGTGCTCTGGTGGAAGTCACCTGATTTTGAATACCATTTTGAAGGCTTCCTATCACGAAAGGATCCAAATAAGAACGATCTTGAATGTATGATGCCTTCTGTTTGGTGGCCTGGTTCATGTGAGGATGTGTCCTACGAAAGTAGCATGCTGTTGACGACTACAGCTTTATCTGAAGCAGTTAATAAGATTGAGGAGAAGCATAGGGACCTCTGTCGCTTGGTCATACAGTTCATCCCACCTATGACACCTCCCCAGTTGCCTGGTTCAGTGTTCAGGACATTTCTACAGAACCTTCTACTTAAGAACAGAGGAGCTGACCGCAATCTTCCACCTCCTGGTGTTTCAAGCAATTCTGTTCTCGTTTCTTTGTACACAGTTATACTTCATTTTCTATCTGAAGGGTTTGGAATGGGGGAAATATGTGGTTGGTTGAAGGGCAGTGAAAATGGTCGTGATGTTGGTTTTCTTCATAGGGGTGGTCATCGGAGCTTTCCAGTAGGTCTATTTCTTAGAAATGATCCCCATCGAAATGACAATACGAGGCTTGGAGGATCTTTTAGTCTCCTCTCAAAATCACACCCTGCAGATGATCAGGAAGCAGAAGATATCCAATGGGAGGAAGGTTGTATGGATGATGAAGAAACCAGAGTGACTCATTTAAGTATAAGAAAACCATGCTGCTGCTCAAGCTATGACGAGGATTTCACAAGAACCTCAAAGTATCCGATTAGATACACAGCTAAAGGTTCACGTGCCCATTGCAGTTCCATGCCAGAGAGATCTTCCCATGTTACCACAGAATGCAATGCTGGAAGTTTGAGTGATGATATAGCTGATAAACCTAGTTCTAGTTACCAATCAGAATCTGATTTTAGTTATTGCCCAGTGCAACATACGAGTTTTATACCAAGGGAGGGTGGTATGTCTTCAGCTACACTCAGAGAAGAAGAACTTCTAGATGTTTTATTGCTGTTGTATCATATAGGTCTAGCGCCAAATTTTAAGCAGGCATCATATCACATGAATCACCAGTTGCAGTCAATAGCCAGTCTGGAAGAAACTGACAAAAAAATCAGAGAAGGACCTTGTTTTGAGCAATTGAAGCATTTGAAAGAAGCTCGTAATGTACATAGAGAAGAGGTCATTGATTCTGTTAGACAATGTGCCTGGTTCCGTATCTCCCTATCCTCTAGATGGAAGCAAAGAGGAATGTATGCAACATGCATGTGGACTGTTCAGTTGCTTCTTGTCCTTAGCAAAGTGGATTTGTTGTTCACTTATGTCCCTGAATACTATCTTGAAGCTCTGGTTGACTGCTTTCATGTGTTGCGTAAGTGTGATCCTCCATTTGTCCCGTCATCAATATTTATCAAGCAAGGCCTAGCTTCATTTATCACCTTTGTGGTTACACATTTCAACGACCCTCGAATATCTAGTGCAGATCTTAGGGATCTGCTTCTCCAATCTATCTCGGTCTTGGTACAGTACAAAGAATATTTGGCTGCGTTTGAGAGCAATGAAGCAGTGAAACAGAGGATGCCAAAGGCATTGCTGTCAGCATTTGATAATAGATCCTGGATCCCAGTAACAAACATTCTTTTGCGTTTGTGCAAGGGATCTGGCTTTGGCTCATCAAAGCATGGAGAATCATCATCGTCATCATCCATCATCTTCCAGAAGTTGTTAGGGCAAACTTGTATCAGTGATGAAGCGTTGTTCTCAGCTTTTCTCAATCGCCTGTTTAACACTCTCAGTTGGACAATGACTGAATTCTCTGTTTCTGTTAGAGAAATGCAAGAAAAGTACCAGGTGTTAGAGTTCCAGCAAAAGAAGTGCAGTGTGATATATGATCTTTCATGCAATCTTGCAAGGGTTTTGGAATTTTGTACTCATGAGATACCTCAAGCATTCCTATCTGGGGCTGATACAAACCTTCGAAGGTTGACTGAGCTGATTGTCTTTATTCTGAACCACATCACTTCAGCAGAAGACACCGAGTTTTTTGACTTGTCTCTGAGACGACATGGTCAATCTTTAGAAAAAGTTAACCGAGGGATGGTATTGGCACCTCTGGTGGGTATTATCTTGAACTTGATAAATGCTAGTGAACAGATGGAATGCAGGGAGCACAATGATGTTGTGAGCATATTTGCAAGCATGGGGTGCCTTGACAGTTTTAATTGCAGATTTCAGTACCTATTGGATTACAACTGGGCTGGAAGTTTTAGAGGCGATGATTATCTCGGAAAACTTTCACAGCTAGAGAATTTTCTGAACCTCATCCTTCTCCGGAGCCAGTCACAAGAAAATAAGATATTAGGGGGAGAAACTGATGTGAATGATGACACTTGCTGCATTTGTTATTCAAGCGAAGCAGATGCTCGGTTTGCCCCTTGTTCCCATAGGTCTTGTTATGGCTGCATAACCAGACATCTTTTGAATTGCCACAGATGCTTCTTTTGCAATGCAACGGTCCTAGATGTTGTCAGGATTAGTGACAAGGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0060154 GO:0065007 GO:0070647 GO:0071704 GO:0098586 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPRY Ufd2P_core zf-C3HC4_3

Protein Analysis

1276

Amino Acids

143.34

Weight (kDa)

5.8

Isoelectric Point (pI)

47.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RKP_N	PF19322	1 - 139	6.4e-59	E3 ubiquitin-protein ligase RKP N-terminus
SPRY	PF00622	150 - 268	5.1e-31	SPRY domain
TPR_RNF123	PF25576	1013 - 1047	3.7e-12	RNF123-like, TPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22010 AT2G22010
fragaria_vesca	FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_1g22150 FvH4_7g04730 FvH4_7g04730
malus_domestica	MD01G1017400.v1.1 MD15G1333600.v1.1
prunus_persica	Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1 Prupe.6G174100_v2.0.a1
pyrus_communis	pycom01g04820 pycom01g04830 pycom15g29160 pycom15g29170
rosa_chinensis	RchiOBHm_Chr2g0116231
rosa_laevigata	RLG00000018249
rosa_multiflora	Rmu_sc0003499.1_g000012 Rmu_sc0004888.1_g000064
rosa_roxburghii	Rroxscaffold_2G00127410
rosa_rugosa	Rorug02G0205000
rosa_samantha	Rh2AG260600 Rh2BG271700 Rh2CG266800 Rh2DG268800
rosa_wichuraiana	Rw2G020430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1199
AasI	GACNNNNNNGTC	1 cut(s)	3342
AatII	GACGTC	1 cut(s)	808
AccB1I	GGYRCC	3 cut(s)	1114, 3382, 3480
AccB7I	CCANNNNNTGG	2 cut(s)	133, 1652
AccBSI	CCGCTC	1 cut(s)	925
AccIII	TCCGGA	1 cut(s)	3606
AciI	CCGC	6 cut(s)	5, 380, 479, 886, 925, 1636
AcuI	CTGAAG	5 cut(s)	570, 1509, 1734, 2229, 3282
AcvI	CACGTG	1 cut(s)	2069
AcyI	GRCGYC	2 cut(s)	805, 1115
AdeI	CACNNNGTG	2 cut(s)	776, 919
AfaI	GTAC	8 cut(s)	1032, 1688, 2445, 2799, 2804, 3122, 3205, 3512
AflII	CTTAAG	1 cut(s)	1616
AjnI	CCWGG	8 cut(s)	1067, 1206, 1426, 1576, 1651, 2483, 2891, 3123
AjuI	GAANNNNNNNTTGG	4 cut(s)	141, 173, 780, 812
AleI	CACNNNNGTG	1 cut(s)	2717
AloI	GAACNNNNNNTCC	2 cut(s)	2573, 2605
Alw21I	GWGCWC	2 cut(s)	1323, 3447
Alw26I	GTCTC	6 cut(s)	700, 949, 1294, 1883, 3328, 3333
AlwNI	CAGNNNCTG	1 cut(s)	2183
Aor13HI	TCCGGA	1 cut(s)	3606
AoxI	GGCC	5 cut(s)	13, 45, 194, 1424, 2695
ApeKI	GCWGC	8 cut(s)	69, 315, 883, 1995, 1998, 2819, 3669, 3735
Asp700I	GAANNNNTTC	3 cut(s)	236, 2268, 3568
AspLEI	GCGC	2 cut(s)	1117, 2311
AspS9I	GGNCC	6 cut(s)	182, 194, 1073, 1522, 2399, 3790
AsuC2I	CCSGG	1 cut(s)	356
AsuHPI	GGTGA	8 cut(s)	74, 371, 554, 644, 788, 1326, 2336, 2704
AsuII	TTCGAA	1 cut(s)	3253
AvaII	GGWCC	5 cut(s)	182, 1073, 1522, 2399, 3790
BaeGI	GKGCMC	2 cut(s)	1253, 2074
BamHI	GGATCC	2 cut(s)	1378, 2895
BanI	GGYRCC	3 cut(s)	1114, 3382, 3480
BarI	GAAGNNNNNNTAC	3 cut(s)	32, 1396, 1428
BbrPI	CACGTG	1 cut(s)	2069
BbsI	GAAGAC	3 cut(s)	15, 2235, 3312
Bbv12I	GWGCWC	2 cut(s)	1323, 3447
BbvI	GCAGC	8 cut(s)	81, 302, 870, 1982, 1985, 2806, 3656, 3722
BceAI	ACGGC	2 cut(s)	28, 367
BcgI	CGANNNNNNTGC	2 cut(s)	3677, 3711
BciT130I	CCWGG	8 cut(s)	1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
BciVI	GTATCC	3 cut(s)	116, 362, 2049
BclI	TGATCA	1 cut(s)	1906
BcnI	CCSGG	1 cut(s)	356
BcoDI	GTCTC	6 cut(s)	700, 949, 1294, 1883, 3328, 3333
BfmI	CTRYAG	4 cut(s)	316, 1476, 1601, 1899
BfoI	RGCGCY	2 cut(s)	1118, 2312
BfrI	CTTAAG	1 cut(s)	1616
BfuI	GTATCC	3 cut(s)	116, 362, 2049
BglII	AGATCT	2 cut(s)	2096, 2758
BisI	GCNGC	9 cut(s)	70, 316, 380, 884, 1996, 1999, 2820, 3670, 3736
BlpI	GCTNAGC	1 cut(s)	400
BlsI	GCNGC	9 cut(s)	71, 317, 381, 885, 1997, 2000, 2821, 3671, 3737
Bme1390I	CCNGG	9 cut(s)	356, 1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
Bme18I	GGWCC	5 cut(s)	182, 1073, 1522, 2399, 3790
BmgT120I	GGNCC	6 cut(s)	182, 194, 1073, 1522, 2399, 3790
BmiI	GGNNCC	9 cut(s)	1074, 1116, 1380, 1523, 2489, 2897, 3384, 3482, 3611
BmrFI	CCNGG	9 cut(s)	356, 1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
BmrI	ACTGGG	4 cut(s)	1564, 2195, 2894, 3538
BmsI	GCATC	8 cut(s)	535, 589, 773, 1398, 2336, 2844, 3685, 3758
BmuI	ACTGGG	4 cut(s)	1564, 2195, 2894, 3538
BoxI	GACNNNNGTC	1 cut(s)	1527
BpiI	GAAGAC	3 cut(s)	15, 2235, 3312
BplI	GAGNNNNNCTC	2 cut(s)	1010, 1042
BpmI	CTGGAG	1 cut(s)	960
Bpu10I	CCTNAGC	1 cut(s)	2570
Bpu1102I	GCTNAGC	1 cut(s)	400
Bpu14I	TTCGAA	1 cut(s)	3253
BpuEI	CTTGAG	2 cut(s)	1987, 3204
BpuMI	CCSGG	1 cut(s)	356
Bsa29I	ATCGAT	1 cut(s)	176
BsaAI	YACGTR	1 cut(s)	2069
BsaBI	GATNNNNATC	4 cut(s)	127, 207, 1839, 3555
BsaHI	GRCGYC	2 cut(s)	805, 1115
BsaJI	CCNNGG	5 cut(s)	355, 1068, 1206, 2225, 3367
BsaWI	WCCGGW	1 cut(s)	3606
BsaXI	ACNNNNNCTCC	2 cut(s)	1029, 1059
Bse118I	RCCGGY	1 cut(s)	196
Bse1I	ACTGG	8 cut(s)	1570, 1811, 2201, 2347, 2364, 2900, 3533, 3613
Bse3DI	GCAATG	6 cut(s)	411, 2074, 2128, 2839, 2864, 3787
Bse8I	GATNNNNATC	4 cut(s)	127, 207, 1839, 3555
BseAI	TCCGGA	1 cut(s)	3606
BseBI	CCWGG	8 cut(s)	1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
BseCI	ATCGAT	1 cut(s)	176
BseDI	CCNNGG	5 cut(s)	355, 1068, 1206, 2225, 3367
BseJI	GATNNNNATC	4 cut(s)	127, 207, 1839, 3555
BseMI	GCAATG	6 cut(s)	411, 2074, 2128, 2839, 2864, 3787
BseMII	CTCAG	7 cut(s)	594, 1289, 2269, 3051, 3082, 3255, 3323
BseNI	ACTGG	8 cut(s)	1570, 1811, 2201, 2347, 2364, 2900, 3533, 3613
BseRI	GAGGAG	4 cut(s)	1031, 1523, 1641, 1871
BseSI	GKGCMC	2 cut(s)	1253, 2074
BseXI	GCAGC	8 cut(s)	81, 302, 870, 1982, 1985, 2806, 3656, 3722
BsgI	GTGCAG	2 cut(s)	2775, 3172
BshFI	GGCC	5 cut(s)	15, 47, 196, 1426, 2697
BshNI	GGYRCC	3 cut(s)	1114, 3382, 3480
BshVI	ATCGAT	1 cut(s)	176
BsiHKAI	GWGCWC	2 cut(s)	1323, 3447
BsiSI	CCGG	3 cut(s)	197, 355, 3607
BslFI	GGGAC	5 cut(s)	302, 792, 1535, 2585, 2654
BsmAI	GTCTC	6 cut(s)	700, 949, 1294, 1883, 3328, 3333
BsmBI	CGTCTC	2 cut(s)	1294, 3328
BsmFI	GGGAC	5 cut(s)	302, 792, 1535, 2585, 2654
BsmI	GAATGC	5 cut(s)	710, 934, 2123, 3224, 3440
BsnI	GGCC	5 cut(s)	15, 47, 196, 1426, 2697
Bsp119I	TTCGAA	1 cut(s)	3253
Bsp1286I	GDGCHC	4 cut(s)	1253, 1323, 2074, 3447
Bsp13I	TCCGGA	1 cut(s)	3606
Bsp1407I	TGTACA	2 cut(s)	1686, 2443
Bsp1720I	GCTNAGC	1 cut(s)	400
BspACI	CCGC	6 cut(s)	5, 380, 479, 886, 925, 1636
BspANI	GGCC	5 cut(s)	15, 47, 196, 1426, 2697
BspCNI	CTCAG	7 cut(s)	595, 1290, 2268, 3050, 3081, 3256, 3324
BspDI	ATCGAT	1 cut(s)	176
BspEI	TCCGGA	1 cut(s)	3606
BspHI	TCATGA	1 cut(s)	3208
BspLI	GGNNCC	9 cut(s)	1074, 1116, 1380, 1523, 2489, 2897, 3384, 3482, 3611
BspMAI	CTGCAG	2 cut(s)	320, 1903
BspT104I	TTCGAA	1 cut(s)	3253
BspT107I	GGYRCC	3 cut(s)	1114, 3382, 3480
BspTI	CTTAAG	1 cut(s)	1616
BsrBI	CCGCTC	1 cut(s)	925
BsrDI	GCAATG	6 cut(s)	411, 2074, 2128, 2839, 2864, 3787
BsrFI	RCCGGY	1 cut(s)	196
BsrGI	TGTACA	2 cut(s)	1686, 2443
BsrI	ACTGG	8 cut(s)	1570, 1811, 2201, 2347, 2364, 2900, 3533, 3613
BssAI	RCCGGY	1 cut(s)	196
BssECI	CCNNGG	5 cut(s)	355, 1068, 1206, 2225, 3367
BssNI	GRCGYC	2 cut(s)	805, 1115
BssT1I	CCWWGG	1 cut(s)	2225
Bst2UI	CCWGG	8 cut(s)	1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
Bst6I	CTCTTC	1 cut(s)	2448
BstACI	GRCGYC	2 cut(s)	805, 1115
BstAFI	CTTAAG	1 cut(s)	1616
BstAPI	GCANNNNNTGC	1 cut(s)	3480
BstAUI	TGTACA	2 cut(s)	1686, 2443
BstBAI	YACGTR	1 cut(s)	2069
BstBI	TTCGAA	1 cut(s)	3253
BstC8I	GCNNGC	5 cut(s)	927, 1113, 1463, 2325, 3472
BstH2I	RGCGCY	2 cut(s)	1118, 2312
BstHHI	GCGC	2 cut(s)	1117, 2311
BstMAI	GTCTC	6 cut(s)	700, 949, 1294, 1883, 3328, 3333
BstMWI	GCNNNNNNNGC	8 cut(s)	385, 931, 2004, 2419, 3480, 3704, 3768, 3777
BstNI	CCWGG	8 cut(s)	1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
BstNSI	RCATGY	6 cut(s)	254, 1215, 1290, 1465, 2541, 2545
BstPAI	GACNNNNGTC	1 cut(s)	1527
BstSCI	CCNGG	9 cut(s)	354, 1067, 1206, 1426, 1576, 1651, 2483, 2891, 3123
BstSFI	CTRYAG	4 cut(s)	316, 1476, 1601, 1899
BstSLI	GKGCMC	2 cut(s)	1253, 2074
BstV1I	GCAGC	8 cut(s)	81, 302, 870, 1982, 1985, 2806, 3656, 3722
BstV2I	GAAGAC	3 cut(s)	15, 2235, 3312
BstX2I	RGATCY	8 cut(s)	1378, 1868, 2096, 2758, 2767, 2888, 2895, 2933
BstXI	CCANNNNNNTGG	1 cut(s)	1577
BstYI	RGATCY	8 cut(s)	1378, 1868, 2096, 2758, 2767, 2888, 2895, 2933
Bsu15I	ATCGAT	1 cut(s)	176
BsuI	GTATCC	3 cut(s)	116, 362, 2049
BsuRI	GGCC	5 cut(s)	15, 47, 196, 1426, 2697
BsuTUI	ATCGAT	1 cut(s)	176
BtgZI	GCGATG	1 cut(s)	3564
BtsI	GCAGTG	4 cut(s)	421, 1762, 2847, 3160
BtsIMutI	CAGTG	9 cut(s)	138, 421, 522, 1590, 1762, 2208, 2847, 3025, 3160
Cac8I	GCNNGC	5 cut(s)	927, 1113, 1463, 2325, 3472
CaiI	CAGNNNCTG	1 cut(s)	2183
CciI	TCATGA	1 cut(s)	3208
CfoI	GCGC	2 cut(s)	1117, 2311
Cfr10I	RCCGGY	1 cut(s)	196
Cfr13I	GGNCC	6 cut(s)	182, 194, 1073, 1522, 2399, 3790
ClaI	ATCGAT	1 cut(s)	176
CsiI	ACCWGGT	1 cut(s)	3123
Csp6I	GTAC	8 cut(s)	1031, 1687, 2444, 2798, 2803, 3121, 3204, 3511
CviQI	GTAC	8 cut(s)	1031, 1687, 2444, 2798, 2803, 3121, 3204, 3511
DinI	GGCGCC	1 cut(s)	1116
DraI	TTTAAA	1 cut(s)	814
DraIII	CACNNNGTG	2 cut(s)	776, 919
DrdI	GACNNNNNNGTC	1 cut(s)	3342
DseDI	GACNNNNNNGTC	1 cut(s)	3342
Eam1104I	CTCTTC	1 cut(s)	2448
EarI	CTCTTC	1 cut(s)	2448
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	1 cut(s)	2225
Eco147I	AGGCCT	1 cut(s)	2697
Eco32I	GATATC	2 cut(s)	675, 1924
Eco47I	GGWCC	5 cut(s)	182, 1073, 1522, 2399, 3790
Eco57I	CTGAAG	5 cut(s)	570, 1509, 1734, 2229, 3282
Eco72I	CACGTG	1 cut(s)	2069
EcoO109I	RGGNCCY	2 cut(s)	1522, 2399
EcoRI	GAATTC	2 cut(s)	79, 3086
EcoRII	CCWGG	8 cut(s)	1067, 1206, 1426, 1576, 1651, 2483, 2891, 3123
EcoRV	GATATC	2 cut(s)	675, 1924
EcoT14I	CCWWGG	1 cut(s)	2225
EcoT22I	ATGCAT	1 cut(s)	2543
EgeI	GGCGCC	1 cut(s)	1116
EheI	GGCGCC	1 cut(s)	1116
ErhI	CCWWGG	1 cut(s)	2225
Esp3I	CGTCTC	2 cut(s)	1294, 3328
FalI	AAGNNNNNCTT	2 cut(s)	2253, 2285
FaqI	GGGAC	5 cut(s)	302, 792, 1535, 2585, 2654
FauI	CCCGC	2 cut(s)	472, 918
FauNDI	CATATG	1 cut(s)	610
FbaI	TGATCA	1 cut(s)	1906
Fnu4HI	GCNGC	9 cut(s)	70, 316, 380, 884, 1996, 1999, 2820, 3670, 3736
Fsp4HI	GCNGC	9 cut(s)	70, 316, 380, 884, 1996, 1999, 2820, 3670, 3736
GlaI	GCGC	2 cut(s)	1116, 2310
GluI	GCNGC	9 cut(s)	70, 316, 380, 884, 1996, 1999, 2820, 3670, 3736
GsuI	CTGGAG	1 cut(s)	960
HaeII	RGCGCY	2 cut(s)	1118, 2312
HaeIII	GGCC	5 cut(s)	15, 47, 196, 1426, 2697
HapII	CCGG	3 cut(s)	197, 355, 3607
HhaI	GCGC	2 cut(s)	1117, 2311
Hin1I	GRCGYC	2 cut(s)	805, 1115
Hin6I	GCGC	2 cut(s)	1115, 2309
HinP1I	GCGC	2 cut(s)	1115, 2309
HincII	GTYRAC	4 cut(s)	1470, 2629, 3261, 3364
HindII	GTYRAC	4 cut(s)	1470, 2629, 3261, 3364
HinfI	GANTC	9 cut(s)	208, 369, 554, 605, 1969, 2180, 2341, 2465, 2963
HpaI	GTTAAC	1 cut(s)	3364
HpaII	CCGG	3 cut(s)	197, 355, 3607
HphI	GGTGA	8 cut(s)	74, 371, 554, 644, 788, 1326, 2336, 2704
Hpy99I	CGWCG	2 cut(s)	88, 109
HpyCH4IV	ACGT	2 cut(s)	805, 2068
HpyF10VI	GCNNNNNNNGC	8 cut(s)	385, 931, 2004, 2419, 3480, 3704, 3768, 3777
HpySE526I	ACGT	2 cut(s)	805, 2068
Hsp92I	GRCGYC	2 cut(s)	805, 1115
HspAI	GCGC	2 cut(s)	1115, 2309
KasI	GGCGCC	1 cut(s)	1114
Kpn2I	TCCGGA	1 cut(s)	3606
Ksp22I	TGATCA	1 cut(s)	1906
KspAI	GTTAAC	1 cut(s)	3364
LmnI	GCTCC	9 cut(s)	922, 1018, 1037, 1050, 1234, 1628, 1803, 3442, 3609
Lsp1109I	GCAGC	8 cut(s)	81, 302, 870, 1982, 1985, 2806, 3656, 3722
LweI	GCATC	8 cut(s)	535, 589, 773, 1398, 2336, 2844, 3685, 3758
MabI	ACCWGGT	1 cut(s)	3123
MaeII	ACGT	2 cut(s)	805, 2068
MaeIII	GTNAC	8 cut(s)	1332, 1966, 2107, 2168, 2722, 2902, 3615, 3812
MbiI	CCGCTC	1 cut(s)	925
MfeI	CAATTG	2 cut(s)	878, 2414
MflI	RGATCY	8 cut(s)	1378, 1868, 2096, 2758, 2767, 2888, 2895, 2933
MhlI	GDGCHC	4 cut(s)	1253, 1323, 2074, 3447
Mly113I	GGCGCC	1 cut(s)	1115
MlyI	GAGTC	2 cut(s)	614, 1963
MmeI	TCCRAC	1 cut(s)	3053
Mph1103I	ATGCAT	1 cut(s)	2543
MroI	TCCGGA	1 cut(s)	3606
MroXI	GAANNNNTTC	3 cut(s)	236, 2268, 3568
MslI	CAYNNNNRTG	7 cut(s)	534, 1285, 1791, 1845, 2542, 2717, 3450
MspCI	CTTAAG	1 cut(s)	1616
MspI	CCGG	3 cut(s)	197, 355, 3607
MspR9I	CCNGG	9 cut(s)	356, 1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
MunI	CAATTG	2 cut(s)	878, 2414
Mva1269I	GAATGC	5 cut(s)	710, 934, 2123, 3224, 3440
MvaI	CCWGG	8 cut(s)	1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
MwoI	GCNNNNNNNGC	8 cut(s)	385, 931, 2004, 2419, 3480, 3704, 3768, 3777
NarI	GGCGCC	1 cut(s)	1115
NciI	CCSGG	1 cut(s)	356
NdeI	CATATG	1 cut(s)	610
NlaIV	GGNNCC	9 cut(s)	1074, 1116, 1380, 1523, 2489, 2897, 3384, 3482, 3611
NmuCI	GTSAC	4 cut(s)	1332, 1966, 3615, 3812
NsiI	ATGCAT	1 cut(s)	2543
NspI	RCATGY	6 cut(s)	254, 1215, 1290, 1465, 2541, 2545
NspV	TTCGAA	1 cut(s)	3253
OliI	CACNNNNGTG	1 cut(s)	2717
PaeI	GCATGC	1 cut(s)	1465
PagI	TCATGA	1 cut(s)	3208
PceI	AGGCCT	1 cut(s)	2697
PctI	GAATGC	5 cut(s)	710, 934, 2123, 3224, 3440
PdmI	GAANNNNTTC	3 cut(s)	236, 2268, 3568
PfeI	GAWTC	7 cut(s)	208, 369, 554, 2180, 2341, 2465, 2963
PflFI	GACNNNGTC	1 cut(s)	3342
PflMI	CCANNNNNTGG	2 cut(s)	133, 1652
PfoI	TCCNGGA	1 cut(s)	2891
PkrI	GCNGC	9 cut(s)	71, 317, 381, 885, 1997, 2000, 2821, 3671, 3737
PleI	GAGTC	2 cut(s)	613, 1963
PluTI	GGCGCC	1 cut(s)	1118
PmaCI	CACGTG	1 cut(s)	2069
PmlI	CACGTG	1 cut(s)	2069
PpsI	GAGTC	2 cut(s)	613, 1963
Ppu21I	YACGTR	1 cut(s)	2069
PpuMI	RGGWCCY	2 cut(s)	1522, 2399
PshAI	GACNNNNGTC	1 cut(s)	1527
PsiI	TTATAA	1 cut(s)	1199
Psp5II	RGGWCCY	2 cut(s)	1522, 2399
Psp6I	CCWGG	8 cut(s)	1067, 1206, 1426, 1576, 1651, 2483, 2891, 3123
PspCI	CACGTG	1 cut(s)	2069
PspGI	CCWGG	8 cut(s)	1067, 1206, 1426, 1576, 1651, 2483, 2891, 3123
PspN4I	GGNNCC	9 cut(s)	1074, 1116, 1380, 1523, 2489, 2897, 3384, 3482, 3611
PspPI	GGNCC	6 cut(s)	182, 194, 1073, 1522, 2399, 3790
PspPPI	RGGWCCY	2 cut(s)	1522, 2399
PstI	CTGCAG	2 cut(s)	320, 1903
PstNI	CAGNNNCTG	1 cut(s)	2183
PsuI	RGATCY	8 cut(s)	1378, 1868, 2096, 2758, 2767, 2888, 2895, 2933
PsyI	GACNNNGTC	1 cut(s)	3342
RsaI	GTAC	8 cut(s)	1032, 1688, 2445, 2799, 2804, 3122, 3205, 3512
RsaNI	GTAC	8 cut(s)	1031, 1687, 2444, 2798, 2803, 3121, 3204, 3511
RseI	CAYNNNNRTG	7 cut(s)	534, 1285, 1791, 1845, 2542, 2717, 3450
SatI	GCNGC	9 cut(s)	70, 316, 380, 884, 1996, 1999, 2820, 3670, 3736
Sau96I	GGNCC	6 cut(s)	182, 194, 1073, 1522, 2399, 3790
SchI	GAGTC	2 cut(s)	614, 1963
ScrFI	CCNGG	9 cut(s)	356, 1069, 1208, 1428, 1578, 1653, 2485, 2893, 3125
SduI	GDGCHC	4 cut(s)	1253, 1323, 2074, 3447
SexAI	ACCWGGT	1 cut(s)	3123
SfaNI	GCATC	8 cut(s)	535, 589, 773, 1398, 2336, 2844, 3685, 3758
SfcI	CTRYAG	4 cut(s)	316, 1476, 1601, 1899
SfoI	GGCGCC	1 cut(s)	1116
SfuI	TTCGAA	1 cut(s)	3253
SinI	GGWCC	5 cut(s)	182, 1073, 1522, 2399, 3790
SmiMI	CAYNNNNRTG	7 cut(s)	534, 1285, 1791, 1845, 2542, 2717, 3450
SmlI	CTYRAG	3 cut(s)	1616, 2002, 3219
SmoI	CTYRAG	3 cut(s)	1616, 2002, 3219
SphI	GCATGC	1 cut(s)	1465
SseBI	AGGCCT	1 cut(s)	2697
SsiI	CCGC	6 cut(s)	5, 380, 479, 886, 925, 1636
SspDI	GGCGCC	1 cut(s)	1114
SspI	AATATT	2 cut(s)	2682, 2813
StuI	AGGCCT	1 cut(s)	2697
StyD4I	CCNGG	9 cut(s)	354, 1067, 1206, 1426, 1576, 1651, 2483, 2891, 3123
StyI	CCWWGG	1 cut(s)	2225
TaiI	ACGT	2 cut(s)	808, 2071
TaqI	TCGA	6 cut(s)	86, 176, 242, 1843, 2743, 3253
TaqII	GACCGA	1 cut(s)	2779
TatI	WGTACW	5 cut(s)	1030, 1686, 2443, 2802, 3203
TauI	GCSGC	1 cut(s)	382
TfiI	GAWTC	7 cut(s)	208, 369, 554, 2180, 2341, 2465, 2963
TscAI	CASTG	9 cut(s)	138, 421, 529, 1590, 1762, 2208, 2847, 3025, 3160
TseFI	GTSAC	4 cut(s)	1332, 1966, 3615, 3812
TseI	GCWGC	8 cut(s)	69, 315, 883, 1995, 1998, 2819, 3669, 3735
Tsp45I	GTSAC	4 cut(s)	1332, 1966, 3615, 3812
TspGWI	ACGGA	3 cut(s)	843, 1065, 2480
TspRI	CASTG	9 cut(s)	138, 421, 529, 1590, 1762, 2208, 2847, 3025, 3160
Tth111I	GACNNNGTC	1 cut(s)	3342
Van91I	CCANNNNNTGG	2 cut(s)	133, 1652
Vha464I	CTTAAG	1 cut(s)	1616
VpaK11BI	GGWCC	5 cut(s)	182, 1073, 1522, 2399, 3790
XbaI	TCTAGA	3 cut(s)	1159, 2272, 2507
XceI	RCATGY	6 cut(s)	254, 1215, 1290, 1465, 2541, 2545
XmnI	GAANNNNTTC	3 cut(s)	236, 2268, 3568
ZraI	GACGTC	1 cut(s)	806
Zsp2I	ATGCAT	1 cut(s)	2543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.